CN110583571A - Construction method of tendinopathy animal model - Google Patents
Construction method of tendinopathy animal model Download PDFInfo
- Publication number
- CN110583571A CN110583571A CN201910872995.3A CN201910872995A CN110583571A CN 110583571 A CN110583571 A CN 110583571A CN 201910872995 A CN201910872995 A CN 201910872995A CN 110583571 A CN110583571 A CN 110583571A
- Authority
- CN
- China
- Prior art keywords
- animal
- tendinopathy
- achilles tendon
- bone cement
- cement block
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 208000000491 Tendinopathy Diseases 0.000 title claims abstract description 31
- 238000010171 animal model Methods 0.000 title claims abstract description 20
- 238000010276 construction Methods 0.000 title claims abstract description 14
- 241001465754 Metazoa Species 0.000 claims abstract description 50
- 210000001361 achilles tendon Anatomy 0.000 claims abstract description 42
- 239000002639 bone cement Substances 0.000 claims abstract description 35
- 238000000034 method Methods 0.000 claims abstract description 18
- 210000002303 tibia Anatomy 0.000 claims abstract description 6
- 238000004519 manufacturing process Methods 0.000 claims abstract description 5
- 238000005520 cutting process Methods 0.000 claims abstract description 4
- 230000002980 postoperative effect Effects 0.000 claims abstract description 4
- 239000004568 cement Substances 0.000 claims description 6
- 241000282693 Cercopithecidae Species 0.000 claims description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 3
- 241000009328 Perro Species 0.000 claims description 3
- 238000002224 dissection Methods 0.000 claims description 3
- 206010033675 panniculitis Diseases 0.000 claims description 3
- 210000004304 subcutaneous tissue Anatomy 0.000 claims description 3
- 201000010099 disease Diseases 0.000 abstract description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 8
- 210000002435 tendon Anatomy 0.000 abstract description 7
- 208000008558 Osteophyte Diseases 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000006378 damage Effects 0.000 description 5
- 102000029816 Collagenase Human genes 0.000 description 3
- 108060005980 Collagenase Proteins 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 3
- 229960002424 collagenase Drugs 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 208000014674 injury Diseases 0.000 description 3
- 239000000835 fiber Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 208000006820 Arthralgia Diseases 0.000 description 1
- 208000036487 Arthropathies Diseases 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 102000001187 Collagen Type III Human genes 0.000 description 1
- 108010069502 Collagen Type III Proteins 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 208000021945 Tendon injury Diseases 0.000 description 1
- 238000001467 acupuncture Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000022159 cartilage development Effects 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002695 general anesthesia Methods 0.000 description 1
- 238000009499 grossing Methods 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 238000002690 local anesthesia Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 208000015122 neurodegenerative disease Diseases 0.000 description 1
- 230000036407 pain Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Prostheses (AREA)
Abstract
The invention relates to a construction method of an animal model with tendinopathy, which comprises the following steps: s10, manufacturing a frustum pyramid-shaped bone cement block; s20, selecting healthy animals with Achilles tendons; s30, cutting a longitudinal incision behind the animal 'S Achilles tendon, and separating the animal' S Achilles tendon bluntly along the animal 'S Achilles tendon to the position below the animal' S Achilles tendon; s40, placing the bone cement block below the achilles tendon of the animal, and binding the bone cement block behind the tibia of the animal by using a fixing line; s50, suturing the incision, and performing postoperative care on the incision position for three to four weeks to obtain the tendinopathy animal model. The invention has the beneficial effects that: the animal tendinopathy model can be stably constructed by the construction method of the animal tendinopathy model, and the method has the advantages of low construction cost, simple operation and strong repeatability; meanwhile, the bone cement block simulates the principle that the bone spurs wear tendons, so the model can well simulate the real state of diseases.
Description
Technical Field
The invention relates to the technical field of construction of animal models with diseases, in particular to a construction method of an animal model with tendinopathy.
Background
Tendinopathy is a disease caused by tendon fatigue damage, and is one of the main causes of joint pain and dysfunction. Tendinopathy is characterized in that the diameter of collagen fiber in the tendon is thinned, meanwhile, the proportion of type III collagen fiber is increased, vascular infiltration occurs in the tendon, and partial hyalinity and chondrogenesis even occur, so that the mechanical property of the tendon is reduced and pain is caused. Meanwhile, as a degenerative disease, the occurrence and development of the disease have close relationship with the aging of the organism. The incidence is 2.1% in the us between 50 and 60 years of age, 5.7% in the us between 60 and 70 years of age, and up to 30% in the us between 70 and 80 years of age. Despite the wide range of tendinopathies, effective treatments for tendinopathies are very limited: the method of shock wave, rehabilitation training and local hormone injection can not cure the tendinopathy, thereby causing a large number of tendinopathy patients to have prolonged illness and causing great loss to individuals and society.
To find an effective therapeutic means, it is important to obtain an animal model that is stable and can most simulate the disease state. At present, the methods for constructing the tendinopathy model mainly comprise the following steps:
1) a needle punching method: the animal achilles tendon is directly punctured and spot checked repeatedly by a needle, so that the achilles tendon structure is damaged, and the achilles tendon is damaged;
2) collagenase injection method: injecting collagenase into the animal achilles tendon by using a chemical principle, thereby causing structural damage to the achilles tendon;
3) a running method comprises the following steps: the animals were placed on a treadmill similar to a treadmill and were allowed to exercise with an overload every day, thereby causing injury to the achilles tendon.
The above methods all have serious disadvantages, and although acupuncture and collagenase injection methods can economically and rapidly cause injury to the achilles tendon, the mechanism and the occurrence mechanism of tendinopathy have great difference, so that the animal model cannot simulate the real state of the disease. Although the treadmill method simulates the tendon injury mechanism to a great extent, the treadmill method has the problems of high manufacturing cost, too long modeling time and unstable model.
Disclosure of Invention
The invention aims to overcome the problems in the prior art and provides a construction method of an animal model with tendinopathy.
In order to achieve the technical purpose and achieve the technical effect, the invention is realized by the following technical scheme:
a construction method of an animal model with tendinopathy comprises the following steps:
s10, manufacturing a frustum pyramid-shaped bone cement block;
s20, selecting healthy animals with Achilles tendons;
s30, cutting a longitudinal incision behind the animal 'S Achilles tendon, and separating the animal' S Achilles tendon bluntly along the animal 'S Achilles tendon to the position below the animal' S Achilles tendon;
s40, placing the bone cement block below the achilles tendon of the animal, and binding the bone cement block behind the tibia of the animal by using a fixing line;
s50, suturing the incision, and performing postoperative care on the incision position for three to four weeks to obtain the tendinopathy animal model.
Furthermore, the bone cement block is formed by pouring and solidifying bone cement, and the cross section of the bone cement block is gradually increased from top to bottom.
Furthermore, the top of the bone cement block is provided with a groove with an arched cross section, and the inner wall of the groove is attached to the bottom of the Achilles tendon of the animal.
Further, the middle part of the fixing wire is embedded in the bone cement block.
Further, the animal is a rabbit, a dog or a monkey.
Further, the blunt dissection employs a curved forceps to separate the animal's achilles tendon from the animal's subcutaneous tissue.
The invention has the beneficial effects that: the animal tendinopathy model can be stably constructed by the construction method of the animal tendinopathy model, and the method has the advantages of low construction cost, simple operation and strong repeatability; meanwhile, the bone cement block simulates the principle that the bone spurs wear tendons, so the model can well simulate the real state of diseases.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:
FIG. 1 is a schematic view of the structure of a cement block, an achilles tendon and a tibia in an embodiment of the present invention;
fig. 2 is a schematic structural view of a cement block and a fixing wire in an embodiment of the present invention.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.
In the description of the present invention, it is to be understood that the terms "opening," "upper," "lower," "thickness," "top," "middle," "length," "inner," "peripheral," and the like are used in an orientation or positional relationship that is merely for convenience in describing and simplifying the description, and do not indicate or imply that the referenced component or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be considered as limiting the present invention.
As shown in fig. 1 and fig. 2, a method for constructing an animal model of tendinopathy includes the following steps:
s10, manufacturing a frustum pyramid-shaped bone cement block 3;
s20, selecting healthy animals with Achilles tendon 1;
s30, cutting a longitudinal incision at the back of the animal achilles tendon 1, and separating the animal achilles tendon 1 along the animal achilles tendon 1 in a blunt manner to the position below the animal achilles tendon 1;
s40, placing the bone cement block 3 below the achilles tendon 1 of the animal, and binding the bone cement block 3 behind the tibia 2 of the animal by using a fixing line 4;
s50, suturing the incision, and performing postoperative care on the incision position for three to four weeks to obtain the tendinopathy animal model.
The bone cement block 3 is formed by pouring and solidifying bone cement, the cross section of the bone cement block 3 is gradually increased from top to bottom, and the bottom surface of the bone cement block 3 is a plane, so that the bone cement block 3 is stably fixed behind the tibia 2. The top of the bone cement block 3 is provided with a groove 31 with an arched cross section, the inner wall of the groove 31 is attached to the bottom of the Achilles tendon 1 of the animal, so that the bone cement block 3 and the Achilles tendon 1 are fixed conveniently, and the Achilles tendon 1 is abraded as soon as possible to cause injury to the Achilles tendon 1.
The middle of the fixation wire 4 is embedded in the bone cement block 3. Before the bone cement block 3 is poured, the middle part of the fixing line 4 is fixed in a mould for pouring the bone cement block 3, so that the fixing line 4 and the bone cement block 3 are integrally poured. Or when the cement block 3 is not completely solidified, pressing the middle part of the fixing line 4 into the rear side of the cement block 3, and then smoothing the surface of the cement block 3.
The animal is rabbit, dog or monkey with low price and easy availability.
Blunt dissection the achilles tendon 1 of the animal was separated from the subcutaneous tissue of the animal using a curved forceps.
Before step S30, the animals are anesthetized either with general anesthesia or with local anesthesia at the achilles tendon 1 portion to be incised.
The animal tendinopathy model can be stably constructed by the construction method of the animal tendinopathy model, and the method has the advantages of low construction cost, simple operation and strong repeatability; meanwhile, the bone cement block simulates the principle that the bone spurs wear tendons, so the model can well simulate the real state of diseases.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed.
Claims (6)
1. A construction method of an animal model with tendinopathy is characterized by comprising the following steps:
s10, manufacturing a frustum pyramid-shaped bone cement block;
s20, selecting healthy animals with Achilles tendons;
s30, cutting a longitudinal incision behind the animal 'S Achilles tendon, and separating the animal' S Achilles tendon bluntly along the animal 'S Achilles tendon to the position below the animal' S Achilles tendon;
s40, placing the bone cement block below the achilles tendon of the animal, and binding the bone cement block behind the tibia of the animal by using a fixing line;
s50, suturing the incision, and performing postoperative care on the incision position for three to four weeks to obtain the tendinopathy animal model.
2. The method of constructing an animal model of tendinopathy according to claim 1, wherein: the bone cement block is formed by pouring and solidifying bone cement, and the cross section of the bone cement block is gradually increased from top to bottom.
3. The method of constructing an animal model of tendinopathy according to claim 1, wherein: the top of the bone cement block is provided with a groove with an arched cross section, and the inner wall of the groove is attached to the bottom of the achilles tendon of the animal.
4. The method of constructing an animal model of tendinopathy according to claim 1, wherein: the middle of the fixation wire is embedded in a cement block.
5. The method of constructing an animal model of tendinopathy according to claim 1, wherein: the animal is rabbit, dog or monkey.
6. The method of constructing an animal model of tendinopathy according to claim 1, wherein: the blunt dissection adopts a curved forceps to separate the achilles tendon of the animal from the subcutaneous tissue of the animal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910872995.3A CN110583571B (en) | 2019-09-16 | 2019-09-16 | Construction method of tendinopathy animal model |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910872995.3A CN110583571B (en) | 2019-09-16 | 2019-09-16 | Construction method of tendinopathy animal model |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110583571A true CN110583571A (en) | 2019-12-20 |
| CN110583571B CN110583571B (en) | 2022-11-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910872995.3A Active CN110583571B (en) | 2019-09-16 | 2019-09-16 | Construction method of tendinopathy animal model |
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| Country | Link |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060286159A1 (en) * | 2003-10-17 | 2006-12-21 | Calvert Murrell George A | Treatment of persistent active tendinopathy using transdermal glyceryl trinitrate providing durability of effect |
| WO2010070971A1 (en) * | 2008-12-16 | 2010-06-24 | アステラス製薬株式会社 | Disease model animal for chronic pain |
| CN102811622A (en) * | 2010-02-22 | 2012-12-05 | 生物模拟治疗公司 | Platelet-derived growth factor compositions and methods for the treatment of tendinopathies |
| BE1020090A5 (en) * | 2012-01-20 | 2013-04-02 | M D Concept | APPARATUS FOR EXTRACORPOREAL SHOCK WAVE TREATMENT. |
| CN103141402A (en) * | 2013-01-29 | 2013-06-12 | 苏州大学 | Electrical stimulating jumping cage for rodent and electronic control system thereof |
| CN204259612U (en) * | 2014-11-21 | 2015-04-15 | 齐齐哈尔医学院 | A kind of laboratory animal electric shock stimulating apparatus |
| CN108742923A (en) * | 2018-03-23 | 2018-11-06 | 云南省第人民医院 | A kind of construction method of osteoporotic fracture disease animal model and application |
| CN109009550A (en) * | 2018-08-31 | 2018-12-18 | 山西医科大学第二医院 | The evaluation method of animal model constructing method, skeletonization drug or material |
-
2019
- 2019-09-16 CN CN201910872995.3A patent/CN110583571B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060286159A1 (en) * | 2003-10-17 | 2006-12-21 | Calvert Murrell George A | Treatment of persistent active tendinopathy using transdermal glyceryl trinitrate providing durability of effect |
| WO2010070971A1 (en) * | 2008-12-16 | 2010-06-24 | アステラス製薬株式会社 | Disease model animal for chronic pain |
| CN102811622A (en) * | 2010-02-22 | 2012-12-05 | 生物模拟治疗公司 | Platelet-derived growth factor compositions and methods for the treatment of tendinopathies |
| BE1020090A5 (en) * | 2012-01-20 | 2013-04-02 | M D Concept | APPARATUS FOR EXTRACORPOREAL SHOCK WAVE TREATMENT. |
| CN103141402A (en) * | 2013-01-29 | 2013-06-12 | 苏州大学 | Electrical stimulating jumping cage for rodent and electronic control system thereof |
| CN204259612U (en) * | 2014-11-21 | 2015-04-15 | 齐齐哈尔医学院 | A kind of laboratory animal electric shock stimulating apparatus |
| CN108742923A (en) * | 2018-03-23 | 2018-11-06 | 云南省第人民医院 | A kind of construction method of osteoporotic fracture disease animal model and application |
| CN109009550A (en) * | 2018-08-31 | 2018-12-18 | 山西医科大学第二医院 | The evaluation method of animal model constructing method, skeletonization drug or material |
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| Publication number | Publication date |
|---|---|
| CN110583571B (en) | 2022-11-29 |
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