CN110577566A - Preparation method of clostridial mycoprotein - Google Patents
Preparation method of clostridial mycoprotein Download PDFInfo
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- CN110577566A CN110577566A CN201910732246.0A CN201910732246A CN110577566A CN 110577566 A CN110577566 A CN 110577566A CN 201910732246 A CN201910732246 A CN 201910732246A CN 110577566 A CN110577566 A CN 110577566A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
Abstract
the invention discloses a preparation method of clostridia mycoprotein, which comprises the following steps: putting the mash and the mother liquor of the first flocculating agent into an air floatation machine, and mixing for the first time and flocculating for the first time to obtain air floatation scum; putting the obtained air flotation scum and the mother liquor of the second flocculating agent into a filtering device, and carrying out secondary mixing and secondary flocculation to obtain a mixed solution; dehydrating and concentrating the mixed solution in the filtering equipment to obtain first concentrated slag; and drying the first concentrated residue in a dryer to obtain the clostridial mycoprotein. The invention develops a method for preparing clostridium mycoprotein by using mash with small particles and high viscosity as a raw material.
Description
Technical Field
The invention belongs to the technical field of mycoprotein preparation, and particularly relates to a preparation method of clostridial mycoprotein.
background
The fuel ethanol is produced by industrial gas biological fermentation method, which is a new high-tech production process, wherein industrial gas is used as a raw material, strains such as Clostridium ethanolate (Clostridium autoethanogenum) or Clostridium butyricum (Clostridium jungdahlii) are used as zymogens, the biological fermentation method is adopted, and the fuel ethanol is produced through the process flows of pretreatment, fermentation, distillation, dehydration and the like, and simultaneously, mash and residual water are produced through distillation. The residual water can be reused, the mash is rich in protein, and the protein can be produced and obtained by processing the mash through separation, drying and the like, thereby obtaining the mycoprotein of Clostridium autoethanolate (Clostridium autoethanogenum) or Clostridium ljungdahlii (Clostridium ljungdahlii).
at present, most of thallus protein liquids contain thallus, moisture and food or other nutrient substances, and centrifugal concentration, multi-effect vacuum concentration and other technologies are mostly adopted for concentration. The mash contains clostridium thalli, salt and water, and the characteristics of high viscosity, small clostridium thalli particles and more fragments cause poor clostridium thalli precipitation performance and filterability; therefore, the clostridium bacterial particles in the mash are small, so that the clostridium bacterial particles cannot be centrifugally concentrated; and because the salt content of mash is high, the vacuum concentration is only adopted to evaporate water, salt and thalli are remained in the final product protein powder, and the protein powder has high ash content and poor quality.
Therefore, there is a need to develop a method for preparing dry clostridial mycoprotein from mash obtained from industrial gas biofermentation method for producing fuel ethanol.
disclosure of Invention
Aiming at the defects of the prior art, the invention provides a preparation method of clostridium mycoprotein, which fills the blank that no preparation method suitable for concentration and drying of clostridium mycoprotein exists in the prior art.
The invention realizes the purpose through the following technical scheme:
A method for preparing clostridial mycoprotein, comprising:
Putting the mash and the mother liquor of the first flocculating agent into an air floatation machine, and mixing for the first time and flocculating for the first time to obtain air floatation scum;
Putting the obtained air flotation scum and the mother liquor of the second flocculating agent into a filtering device, and carrying out secondary mixing and secondary flocculation to obtain a mixed solution;
Dehydrating and concentrating the mixed solution in the filtering equipment to obtain first concentrated slag;
And drying the first concentrated residue in a dryer to obtain the clostridial mycoprotein.
Further, the clostridium is clostridium ethanolicum or clostridium ljungdahlii.
further, the filtering equipment is a screw stacking machine or a belt filter press.
Further, before the mash and the first flocculant mother liquor are put into the air flotation machine,
Preparing a first flocculating agent mother solution,
And preparing a second flocculant mother liquor.
The flocculant used for preparing the mother liquor of the first flocculant is one or more of polyacrylamide, chitosan, sodium carboxymethyl cellulose, polyaluminium sulfate, sodium polyacrylate and the like, and the flocculant used for preparing the mother liquor of the second flocculant is one or more of polyacrylamide, chitosan, sodium carboxymethyl cellulose, polyaluminium sulfate, sodium polyacrylate and the like.
Further, the mass concentration of the first flocculant mother liquor is 0.05-0.5%, and the mass concentration of the second flocculant mother liquor is 0.05-0.5%.
Further, the temperature for preparing the first flocculant mother liquor is controlled to be 35-55 ℃, and the temperature for preparing the second flocculant mother liquor is controlled to be 35-55 ℃.
Further, the volume of the first flocculant mother liquor is 3-5% of the mash; and the volume of the mother liquor of the second flocculating agent is 2-5% of that of the air flotation scum.
Further, the primary flocculation temperature is controlled to be 30-50 ℃, and the secondary flocculation temperature is controlled to be 30-50 ℃.
Further, the drying the first concentrated slag in a dryer to obtain the clostridial mycoprotein comprises,
Drying the first concentrated slag in a vacuum paddle dryer or a rake dryer to obtain second concentrated slag;
and drying the second concentrated residue in flash evaporation drying equipment to obtain the clostridial mycoprotein.
Furthermore, the water content of the second concentrated slag is 35-55%.
the beneficial effects of the invention at least comprise:
A method for preparing clostridial mycoprotein, comprising: putting the mash and the flocculant mother liquor into an air flotation machine, mixing and flocculating to obtain air flotation scum; putting the obtained air flotation scum and the flocculant mother liquor into a filtering device, mixing and flocculating to obtain a mixed solution; dehydrating and concentrating the mixed solution in the filtering equipment to obtain first concentrated slag; and drying the first concentrated slag in a dryer to obtain mycoprotein powder. The method combines an air flotation machine, a filtering device and a dryer, and successfully prepares the clostridial mycoprotein in mash only containing thalli, salt and water.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a process diagram of an embodiment of a method for producing clostridial mycoprotein.
Detailed Description
the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the invention provides a preparation method of clostridia mycoprotein, figure 1 is a process step diagram of the preparation method embodiment of the clostridia mycoprotein, and with reference to figure 1, the method comprises the following steps:
S1, preparing a first flocculant mother liquor and preparing a second flocculant mother liquor. The flocculating agent used for preparing the mother liquor of the first flocculating agent and the mother liquor of the second flocculating agent is one or more of polyacrylamide, chitosan, sodium carboxymethyl cellulose, polyaluminium sulfate, sodium polyacrylate and the like. When the flocculant is prepared, the flocculant is slowly scattered into water, and the flocculant is agglomerated and insufficiently dissolved due to excessive scattering at one time, so that the waste of the flocculant is caused and the quality of clostridium mycoprotein is influenced; the water is preferably neutral and does not contain salts or impurities, because the charged ions such as calcium, magnesium and the like in the water can be combined with the flocculating agent to influence the combination of the flocculating agent and the clostridial mycoprotein, thereby influencing the flocculation effect of the flocculating agent; the preparation temperature is controlled to be 35-55 ℃, the dissolution of the flocculating agent can be accelerated in the temperature range, the flocculating agent can be uniformly dissolved, and the temperature range is close to the temperature of the mash, so that the influence on the temperature of the mash when the mother liquor of the flocculating agent is added into the mash can be avoided, and good temperature conditions are provided for primary flocculation and secondary flocculation; the mass concentration of the obtained first flocculant mother liquor is 0.05-0.5%, the mass concentration of the second flocculant mother liquor is 0.05-0.5%, the concentration of the flocculant mother liquor can not be too high or too low, if the concentration of the flocculant mother liquor is too high, the viscosity of the flocculant mother liquor is high, so that a pump body cannot press the flocculant mother liquor into liquid needing flocculation, and the flow of the flocculant mother liquor is difficult to control; if the concentration of the mother liquid of the flocculating agent is too small, the excessive water in the flocculating agent is shown, the load is generated on the filtering process, the water resource is wasted, more mother liquid of the flocculating agent is needed by the same amount of liquid for flocculation, the capacity of the pump body is limited at the moment, and the requirement of flocculation cannot be met.
S2, placing the mash and the first flocculant mother liquor into an air flotation machine for primary mixing and primary flocculation to obtain air flotation scum. The main practical operation of the step is that the mash and the mother liquor of the first flocculating agent are placed into an air flotation groove of an air flotation machine, the clostridial mycoprotein in the mash is fully contacted with the mother liquor of the first flocculating agent and flocculated under the air flotation action of the air flotation machine, and after the flocculation is finished, an air flotation scraper scrapes air flotation scum into a buffer groove. The step is used for dehydrating the mash for one time, so that the dehydration pressure of subsequent filtering equipment is reduced.
Wherein the volume of the first flocculant mother liquor is 3-5% of the mash, the primary flocculation temperature is controlled to be 30-50 ℃, and under the temperature range, the clostridium mycoprotein in the mash has good flocculation effect, is solid in flocculation and is easy to separate; and because the temperature of the obtained mash is about 55 ℃, the mash does not need to be additionally heated and excessively cooled, and the energy is saved. The pressure of the flocculating agent and the mash is controlled to be 0.7-0.8 MPa, which is to ensure that the flocculating agent and mycoprotein in the mash are fully contacted to flocculate and agglomerate; the speed and height of the air-floating scraper blade are properly adjusted according to the concentration of mash and the size of the flocculation group, so that the bacterial protein flocculation group can be scraped out, and the clostridium bacterial protein flocculation group is prevented from being broken.
In the common water treatment of the air flotation technology, highly dispersed micro bubbles are formed in water, solid or liquid particles of hydrophobic groups in wastewater are adhered to form a water-gas-particle three-phase mixed system, and after the bubbles are adhered to the particles, flocs with apparent density smaller than that of the water are formed and float to the water surface, and a scum layer is formed and scraped, so that the solid-liquid or liquid-liquid separation process is realized. The invention applies flocculation and air flotation technology to the preparation of clostridium mycoprotein, can ensure that a flocculating agent is fully contacted with the protein and flocculates the protein, and a protein flocculation group floats on the liquid surface under the action of air flotation, thereby being convenient for scraping out by an air flotation scraper and removing most of water. The air floatation equipment is simple, the investment cost is low, the operation energy consumption is low, and the protein with small particle size, such as clostridium thallus, can be effectively gathered together, thereby being convenient for subsequent separation.
And S3, placing the air flotation scum obtained in the step S2 and the mother liquor of the second flocculating agent into a filtering device for secondary mixing and secondary flocculation to obtain a mixed liquor.
the step is mainly to carry out secondary flocculation on the air flotation scum subjected to primary dehydration so as to prepare for further dehydration.
Wherein the volume of the mother liquor of the second flocculating agent is 2-5% of the volume of the air flotation scum, and the temperature of the secondary flocculation is controlled to be 30-50 ℃. Under the temperature range, the clostridium mycoprotein in the air floatation scum has good flocculation effect, is firmly flocculated and is easy to separate.
And S4, dehydrating and concentrating the mixed liquor obtained in the step S3 in a filtering device to obtain first concentrated slag. At this time, most of the water in the mixed liquor is removed, the first concentrated slag has no flowability basically, and the conditions for drying and preparing the protein are met.
In this step, the filtration apparatus is a stack screw or a belt filter press.
the main body of the screw stacking machine is formed by mutually stacking a fixed ring and a movable ring, a spiral shaft penetrates through a filtering device formed in the spiral shaft, the front section is a concentration part, and the rear section is a dewatering part. The mash is transported to a dewatering part after being concentrated by gravity in a concentrating part of the stack screw machine, and generates great internal pressure along with the gradual reduction of a filter seam and a screw pitch in the advancing process and the blocking effect of a back pressure plate, so that the volume is continuously reduced, and the aim of fully dewatering is fulfilled. The filter seam formed between the fixed ring and the movable ring and the screw pitch of the screw shaft are gradually reduced from the concentration part to the dehydration part. The rotation of the spiral shaft pushes mash from the concentration part to the dewatering part and simultaneously drives the movable ring to clean the filter seam, thereby preventing blockage.
And (3) placing the air flotation scum and the mother liquor of the second flocculating agent into a screw stacking machine for secondary mixing and secondary flocculation, wherein in actual operation, the air flotation scum and the mother liquor of the second flocculating agent are directly added into a feed pipeline to be mixed through a pipeline, and pass through a two-stage mixing tank, the stirring speed of the first-stage mixing tank is controlled to be 10rpm, the gap of the second-stage mixing tank is opened, and the stirring is opened for 5min every 15min, and the rotating speed is controlled to be 5 rpm. The gap opening of the secondary mixing tank improves the flocculation rate of the clostridial mycoprotein, and simultaneously avoids the destruction of the clostridial mycoprotein flocculation group obtained from the primary mixing tank. The rotating speed of the spiral shaft is variable frequency, the frequency is adjusted according to the clear water outlet liquid, and if the water outlet liquid is turbid, the rotating speed of the spiral shaft is reduced until the water outlet liquid is clear. The front section of the stacked spiral shell is dewatered by gravity, the rear end of the stacked spiral shell is dewatered by extrusion, the extrusion pressure is controlled by adjusting the gap of the back pressure plates of the stacked spiral shell, and if the effluent is turbid, the gap of the back pressure plates is enlarged until the effluent is clear. The operation can avoid the disruption of the clostridium mycoprotein flocculation group and improve the yield of the clostridium mycoprotein.
The dewatering process of the belt press filter can be divided into four important stages of pretreatment, gravity dewatering, wedge-shaped area prepressing dewatering and press dewatering. The flocculated material is gradually added onto the filter belt, so that the free water outside the floc is separated from the floc under the action of gravity, and the water content of the sludge floc is gradually reduced. After the sludge is dewatered by gravity, the sludge enters a wedge-shaped prepressing dewatering section to remove the free water on the surface of the sludge by slight extrusion, and the sludge is pressed and dewatered again after meeting the pressing and dewatering requirements, thereby realizing the purpose of gradual dewatering.
the mash in the invention is a liquid obtained by distilling fermentation liquor obtained in the process of producing fuel ethanol by fermenting industrial gas and collecting alcohol. Compared with the conventional thallus protein liquid, the liquid has fine particles, and the air floatation scum is flocculated again before entering a stack screw or belt filter press, so that the protein flocculation groups can be obviously increased, and the secondary dehydration and separation are convenient. If the conventional centrifugal concentration treatment needs additional buffer equipment and corresponding material transportation pump equipment, the investment cost is increased, and protein flocculation in mash can be damaged in pipeline and pump transportation after the mash is flocculated, so that the centrifugal separation effect is influenced. Due to the high-speed rotation of the centrifugal equipment, the protein groups are easily broken again, and the separation is difficult; meanwhile, the protein is attached to the centrifugal drum or the disc, so that equipment corrosion is easily caused and the centrifugal effect is further reduced; centrifugal separation needs to be stopped frequently to clean equipment, influences production efficiency, and it has "self-cleaning" function to fold spiral shell machine, can continuous production, and production efficiency is high. If a filter pressing device such as a plate frame is adopted, the protein flocculation is crushed quickly due to high pressure, so that the filter cloth is blocked, and the dehydration is difficult. By adopting the belt type filter press, the water can be gradually removed, the filter pressing is low, and meanwhile, the filter cloth can be continuously cleaned on line, so that the filter cloth is prevented from being blocked. Due to the special fermentation process for preparing ethanol by fermenting industrial tail gas, the ash content of the product is high after vacuum concentration, and the quality of the product is influenced, so that the special fermentation process is not suitable for using vacuum concentration equipment.
and S5, drying the first concentrated slag in a vacuum paddle dryer or a rake dryer to obtain second concentrated slag.
The water content of the second concentrated slag is controlled to be 35-55%, because if the water content of the second concentrated slag is too high, the investment cost of subsequent flash evaporation equipment is high, and the drying cost is high due to low thermal efficiency of flash evaporation drying. If the water content of the second concentrated slag is too low, the second concentrated slag stays in the vacuum paddle dryer or the rake dryer for a long time, so that part of materials are easily contacted with the heat exchange part for a long time, and the quality of the clostridial mycoprotein is influenced.
and S6, drying the second concentrated residue in flash evaporation drying equipment to obtain mycoprotein. The water content of the obtained mycoprotein is less than or equal to 12 percent.
Further, the clostridial mycoprotein is clostridial ethanolate mycoprotein or clostridial young mycoprotein.
The invention takes industrial mash as raw material, the mash is flocculated, then clostridium mycoprotein is prepared by creatively adopting a method of combining an air flotation machine, a filtering device and a dryer, compared with the existing centrifugal concentration and vacuum concentration technologies, the method only needs to put in air flotation and normal pressure filtering devices, does not need to put in excessive equipment such as pipelines and pumps, saves investment cost and operation energy consumption, has high automation degree and less personnel demand during operation, does not need frequent CIP cleaning during operation, can continuously and stably operate for a long time, and has high production efficiency and small floor area. The invention adopts two drying methods, namely drying the first concentrated slag in a vacuum paddle dryer or a rake dryer to evaporate most of water to obtain second concentrated slag, and drying the second concentrated slag in flash drying equipment for the second time, so that the drying cost and the energy consumption are lower than those of pure flash drying, the production interruption caused by frequent shutdown of cleaning equipment is avoided, the production efficiency is high, the personnel demand is less, and the equipment maintenance cost is low.
The method for producing a clostridial mycoprotein according to the present invention will now be described with reference to the specific examples.
Example 1
In the embodiment, industrial coal gas is used as a raw material, industrial mash obtained in a process of preparing fuel gas ethanol by using clostridium ethanolicum as zymocyte is used as a raw material, polyacrylamide is used as a flocculating agent to prepare a first flocculating agent mother solution, polyacrylamide is used as a flocculating agent to prepare a second flocculating agent mother solution, the preparation temperatures of the first flocculating agent mother solution and the second flocculating agent mother solution are both controlled to be 50 ℃, the volume of the first flocculating agent mother solution is 3% of the volume of the mash, and the primary flocculation temperature is controlled to be 42 ℃; the volume of the mother liquor of the second flocculating agent is 3 percent of that of the air flotation scum, and the temperature of the secondary flocculation is controlled to be 38 ℃, so as to obtain mixed liquor; and overflowing the mixed solution into a screw stacking machine, dehydrating and concentrating to obtain first concentrated slag, drying the first concentrated slag in a vacuum paddle dryer to obtain second concentrated slag, wherein the water content of the second concentrated slag is 38%. And drying the second concentrated residue in flash evaporation drying equipment to obtain the clostridial mycoprotein.
It is noted that, the content of crude protein in the clostridium ethanolicum mycoprotein prepared in the embodiment is more than or equal to 80 percent, the water content is less than or equal to 12 percent, and the ash content is less than or equal to 7 percent through tests.
Example 2
in another embodiment of the present specification, the preparation temperature of the first flocculant mother liquor and the preparation temperature of the second flocculant mother liquor are controlled to 35 ℃, the volume of the first flocculant mother liquor is 4% of the volume of the mash, the primary flocculation temperature is controlled to 50 ℃, the volume of the second flocculant mother liquor is 4% of the volume of the air flotation scum, the volume of the second flocculant mother liquor is 3% of the volume of the air flotation scum, the secondary flocculation temperature is controlled to 50 ℃, and the water content of the second concentrated scum is 50%, which is different from example 1.
It is noted that the crude protein content, the moisture content of 10.1%, and the ash content of 5.2% in the clostridium ethanolicum mycoprotein prepared in this example were tested.
Example 3
As another example, different from example 2, a mash obtained by preparing ethanol by using clostridium ljunii as zymocyte is used as a raw material, and chitosan is used as a flocculant to prepare a first flocculant mother liquor and a second flocculant mother liquor.
It is noted that the crude protein content of the young clostridium mycoprotein prepared in the example is 82.3%, the water content is 9.1%, and the ash content is 6.5% through tests.
example 4
As yet another example of the present description, unlike example 2, a first flocculant mother liquor and a second flocculant mother liquor were formulated using sodium methyl cellulose as the flocculant.
It is noted that the crude protein content of the clostridium ethanolicum mycoprotein prepared in the example is 81.5%, the water content is 11.1% and the ash content is 6.1%.
Example 5
As a further example of the present specification, in contrast to example 2, polyaluminium sulfate was used as a flocculant to formulate a first flocculant mother liquor and a second flocculant mother liquor.
It is noted that the crude protein content of the clostridium ethanolicum mycoprotein prepared in the example is 80.2%, the water content is 11.7%, and the ash content is 6.7%.
Example 6
As yet another example of the present specification, unlike example 2, a first flocculant mother liquor and a second flocculant mother liquor were formulated using sodium polyacrylate as the flocculant.
it is noted that the crude protein, water content and ash content of the clostridium ethanolicum mycoprotein prepared in the example are 85.2%, 9.7% and 4.7% respectively.
Example 7
Different from the embodiment 6, the mash obtained by preparing ethanol by using the clostridium ljungdahlii as the zymocyte is used as the raw material, and the filtering equipment is a belt filter press.
It is noted that the young clostridium mycoprotein prepared in the example is tested to have 80.7% of crude protein, 9.7% of water and 6.1% of ash.
Example 8
different from the embodiment 1, the first concentrated slag is placed in a rake dryer to be dried to obtain second concentrated slag.
It is noted that the crude protein, water content and ash content of the clostridium ethanolicum mycoprotein prepared in the example are respectively 84.7%, 9.2% and 3.8% through tests.
the above-mentioned embodiments are only for convenience of description, and are not intended to limit the present invention in any way, and it will be understood by those skilled in the art that the technical features of the present invention can be changed or modified by the equivalent embodiments without departing from the scope of the present invention.
Claims (10)
1. A method for preparing clostridial mycoprotein, comprising:
Putting the mash and the mother liquor of the first flocculating agent into an air floatation machine, and mixing for the first time and flocculating for the first time to obtain air floatation scum;
Putting the obtained air flotation scum and the mother liquor of the second flocculating agent into a filtering device, and carrying out secondary mixing and secondary flocculation to obtain a mixed solution;
Dehydrating and concentrating the mixed solution in the filtering equipment to obtain first concentrated slag;
And drying the first concentrated residue in a dryer to obtain the clostridial mycoprotein.
2. The method according to claim 1, wherein the clostridial mycoprotein is a clostridial ethanolate mycoprotein or a clostridial young mycoprotein.
3. The method according to claim 1, wherein the filtration apparatus is a stack screw or a belt filter press.
4. The method of claim 1, further comprising the step of introducing the mash and the first flocculant mother liquor into an air flotation machine simultaneously,
Preparing a first flocculating agent mother solution,
Preparing a mother solution of a second flocculating agent,
The flocculant used for preparing the mother liquor of the first flocculant is one or more of polyacrylamide, chitosan, sodium carboxymethyl cellulose, polyaluminium sulfate, sodium polyacrylate and the like, and the flocculant used for preparing the mother liquor of the second flocculant is one or more of polyacrylamide, chitosan, sodium carboxymethyl cellulose, polyaluminium sulfate, sodium polyacrylate and the like.
5. The method for preparing clostridial mycoprotein as claimed in claim 4, wherein the first flocculating agent mother liquor has a mass concentration of 0.05-0.5%, and the second flocculating agent mother liquor has a mass concentration of 0.05-0.5%.
6. The method for preparing clostridial mycoprotein as claimed in claim 4, wherein the temperature of the first flocculant mother liquor is controlled to be 35-55 ℃ and the temperature of the second flocculant mother liquor is controlled to be 35-55 ℃.
7. the method of claim 1, wherein the volume of the first flocculant mother liquor is 3-5% of the mash, and the volume of the second flocculant mother liquor is 2-5% of the air flotation scum.
8. The method for preparing clostridial mycoprotein as claimed in claim 1, wherein the primary flocculation temperature is controlled to be 30-50 ℃ and the secondary flocculation temperature is controlled to be 30-50 ℃.
9. The method of claim 1, wherein drying the first concentrate in a dryer to obtain the clostridial mycoprotein comprises,
drying the first concentrated slag in a vacuum paddle dryer or a rake dryer to obtain second concentrated slag;
And drying the second concentrated residue in flash evaporation drying equipment to obtain the clostridial mycoprotein.
10. The method of claim 9, wherein the second concentrate has a moisture content of 35-55%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113788570A (en) * | 2021-10-28 | 2021-12-14 | 宁夏滨泽新能源科技有限公司 | Method for extracting mycoprotein in disc centrifuge cleaning water |
| CN114292966A (en) * | 2021-12-23 | 2022-04-08 | 巴彦淖尔华恒生物科技有限公司 | Separation method and application of flocculation protein in starch sugar solution |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113788570A (en) * | 2021-10-28 | 2021-12-14 | 宁夏滨泽新能源科技有限公司 | Method for extracting mycoprotein in disc centrifuge cleaning water |
| CN114292966A (en) * | 2021-12-23 | 2022-04-08 | 巴彦淖尔华恒生物科技有限公司 | Separation method and application of flocculation protein in starch sugar solution |
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