CN110563939A - Method for preparing phenylalanine oligopeptide-monomethoxypolyethylene glycol copolymer by enzyme catalysis - Google Patents
Method for preparing phenylalanine oligopeptide-monomethoxypolyethylene glycol copolymer by enzyme catalysis Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G65/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
- C08G65/02—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from cyclic ethers by opening of the heterocyclic ring
- C08G65/32—Polymers modified by chemical after-treatment
- C08G65/329—Polymers modified by chemical after-treatment with organic compounds
- C08G65/333—Polymers modified by chemical after-treatment with organic compounds containing nitrogen
- C08G65/33303—Polymers modified by chemical after-treatment with organic compounds containing nitrogen containing amino group
- C08G65/33317—Polymers modified by chemical after-treatment with organic compounds containing nitrogen containing amino group heterocyclic
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G65/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
- C08G65/02—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from cyclic ethers by opening of the heterocyclic ring
- C08G65/32—Polymers modified by chemical after-treatment
- C08G65/329—Polymers modified by chemical after-treatment with organic compounds
- C08G65/333—Polymers modified by chemical after-treatment with organic compounds containing nitrogen
- C08G65/33331—Polymers modified by chemical after-treatment with organic compounds containing nitrogen containing imide group
- C08G65/33337—Polymers modified by chemical after-treatment with organic compounds containing nitrogen containing imide group cyclic
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G65/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
- C08G65/02—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from cyclic ethers by opening of the heterocyclic ring
- C08G65/32—Polymers modified by chemical after-treatment
- C08G65/329—Polymers modified by chemical after-treatment with organic compounds
- C08G65/333—Polymers modified by chemical after-treatment with organic compounds containing nitrogen
- C08G65/33396—Polymers modified by chemical after-treatment with organic compounds containing nitrogen having oxygen in addition to nitrogen
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- C12P21/00—Preparation of peptides or proteins
Abstract
the invention relates to a method for preparing a phenylalanine oligopeptide-monomethoxy polyethylene glycol copolymer through enzyme catalysis, which comprises the following steps of adding phenylalanine oligopeptide, oxypolyethylene glycol succinimide carbonate and N, N-dimethylaniline into anhydrous methanol to react under the protection of inert gas, adding precooled methanol after the reaction is finished, carrying out vacuum filtration, drying the filtrate in vacuum, adding preheated acetone until the filtrate is completely dissolved, standing the obtained solution overnight, carrying out vacuum filtration, and obtaining a precipitate as the phenylalanine oligopeptide-monomethoxy polyethylene glycol copolymer.
Description
Technical Field
The invention relates to a method for preparing a phenylalanine oligopeptide-monomethoxy polyethylene glycol copolymer through enzyme catalysis, and belongs to the technical field of synthetic methods of phenylalanine oligopeptide-monomethoxy polyethylene glycol copolymers.
Background
the artificial polypeptide is a polyamino compound formed by taking natural amino acid as a monomer to carry out polymerization reaction through amido bond, and the properties of the artificial polypeptide are similar to those of natural peptide, polyamino acid and protein. The material formed by the artificial polypeptide has good biocompatibility and degradability. The polypeptide or the modified oligomer thereof shows unique structural properties such as self-assembly, liquid crystal behavior and the like, has stronger plasticity, and has great application potential in the fields of biology, medicine and natural high polymer materials. Oligomeric phenylalanine is modified by polyethylene glycol or copolymerized with glutamic acid, lysine and the like to prepare amphiphilic macromolecules for drug embedding, gene vectors and the like.
disclosure of Invention
The invention aims to overcome the defects in the prior art and provide the method for preparing the phenylalanine oligopeptide-monomethoxy polyethylene glycol copolymer through enzyme catalysis, which has the advantages of mild reaction conditions, environmental friendliness, less side reactions and avoidance of complicated protection and deprotection steps.
According to the technical scheme provided by the invention, the method for preparing the phenylalanine oligopeptide-monomethoxypolyethylene glycol copolymer through enzyme catalysis comprises the following steps:
a1adding 2 ~ 5g of phenylalanine oligopeptide, oxy polyethylene glycol succinimide carbonate and N, N ~ dimethylaniline into 10 ~ 20ml of anhydrous methanol, wherein the weight ratio of the phenylalanine oligopeptide, the oxy polyethylene glycol succinimide carbonate and the N, N ~ dimethylaniline is 1 (1 ~ 2) to (8 ~ 12), and reacting the reaction mixture for 10 ~ 36 h at 30 ~ 50 ℃ under the protection of inert gas;
b1After the reaction is finished, adding 100-150ml of methanol precooled to-5-0 ℃, and carrying out vacuum filtration;
c1firstly, the filtrate is treated with 30-vacuum drying at 50 deg.C for 6-10 hr, adding acetone preheated to 40-52 deg.C until it is completely dissolved;
d1And standing the obtained solution at-5-0 ℃ overnight, and carrying out vacuum filtration to obtain a precipitate, namely the phenylalanine oligopeptide-monomethoxypolyethylene glycol copolymer.
Step b1the range of the air pressure after decompression is 0 ~ 10Kpa, and the time of suction filtration is 0.5 ~ 1 hour.
step d1the range of the air pressure after decompression is 0 ~ 10Kpa, and the time of suction filtration is 0.5 ~ 1 hour.
the synthesis method of the phenylalanine oligopeptide comprises the following steps:
a2adding 0.5 ~ 2.0 g of L ~ phenylalanine methyl ester hydrochloride, dimethyl sulfoxide and 2 ~ 5U protease into 2.0 ~ 3.0 mL of disodium hydrogen phosphate ~ citric acid buffer solution, placing a reaction mixture in a constant temperature oscillator, and reacting at 500 ~ 700 rpm and 30 ~ 70 ℃ for 5 ~ 10 hours, wherein the disodium hydrogen phosphate ~ citric acid buffer solution contains 0.1 ~ 0.2M of disodium hydrogen phosphate ~ citric acid, the pH value of the disodium hydrogen phosphate ~ citric acid buffer solution is 6.5 ~ 8.5, and the volume of the dimethyl sulfoxide is 20 ~ 50% of the volume of the phosphate buffer solution;
b2after the reaction is finished, centrifuging the reaction mixture for 2 ~ 4 minutes at 8000 ~ 12000rpm to obtain a precipitate;
c2and washing the precipitate with deionized water and glacial ethanol for 2 ~ 3 times respectively, and freeze ~ drying to obtain the phenylalanine oligopeptide.
Step a2the protease is one of bromelain, papain, trypsin, neutral protease and alkaline protease.
The preparation of methoxypolyethylene glycol succinimide carbonate adopts the following steps:
a3adding 1 ~ 2g of methoxypolyethylene glycol, 5 ~ 8 g of N, N' ~ disuccinimidyl carbonate and 3 ~ 6g of 4 ~ (dimethylamino) pyridine into 25 mL of anhydrous tetrahydrofuran, and reacting the reaction mixture at 25 ~ 35 ℃ for 4 ~ 8 hours under the condition of stirring at the speed of 150 ~ 250 rpm;
b3after the reaction is finished, the reaction mixture isvacuum distilling at 35 ~ 45 ℃ to remove anhydrous tetrahydrofuran;
c3Pouring the reaction mixture into precooled anhydrous ether, carrying out suction filtration under reduced pressure to collect precipitate, and obtaining the precipitate which is methoxy polyethylene glycol succinimide carbonate.
step a3The average molecular weight of the methoxypolyethylene glycol is 200 Da, 400 Da or 1000 Da.
Step b3the vacuum degree during vacuum distillation is controlled ~ be 0 ~ 10 Kpa.
Step c3in the method, the precooling temperature of the anhydrous ether is ~ 5 ~ 0 ℃, and the pressure of the reduced pressure suction filtration is controlled to be 0 ~ 10 Kpa.
The method has the advantages of mild reaction conditions, more environment-friendly property, less side reaction and the like, and avoids fussy protection and deprotection steps;
the yield of the phenylalanine oligopeptide ~ monomethoxypolyethylene glycol copolymer prepared by the method is 30 ~ 45%, and the substitution degree of the phenylalanine oligopeptide ~ monomethoxypolyethylene glycol copolymer detected by a 1HNMR means is 1.01 ~ 1.1.
Detailed Description
The present invention will be further described with reference to the following specific examples.
the detection method of the 1HNMR comprises the following steps: the copolymer was measured at room temperature using an AVANCE III HD-400 MHz NMR spectrometer with CF3COOD as the solvent and Tetramethylsilane (TMS) as the internal standard1HNMR spectrogram.
example 1
1. Protease catalyzed synthesis of phenylalanine oligopeptide
a20.5 g of L-phenylalanine methyl ester hydrochloride (national drug group chemical reagent Co., Ltd.), dimethyl sulfoxide (national drug group chemical reagent Co., Ltd.) and 2U of trypsin (Shanghai Vocko Biotechnology Co., Ltd.) were added to 2.0 mL of a disodium hydrogen phosphate-citric acid buffer solution (Nanchang rain and dew laboratory instruments Co., Ltd.) at pH 6.5. The volume fraction of dimethyl sulfoxide was 20% phosphate buffer. The reaction mixture was placed in a constant temperature shaker (600rpm) and reacted at 30 ℃ for 5 hours;
b2after the reaction is finished, centrifuging the reaction mixture for 3 minutes at 10000 rpm to obtain a precipitate;
c2And washing the precipitate twice with deionized water and glacial ethanol respectively, and freeze-drying to obtain the product phenylalanine oligopeptide.
2. Preparation of Monomethoxypolyethylene glycol succinimide carbonate
a31 g of monomethoxypolyethylene glycol (Huaian petrochemical plant, Huai province, Jiangsu), 5g of N, N' -disuccinimidyl carbonate (Huai pharmaceutical chemical Co., Ltd.), and 3g of 4- (dimethylamino) pyridine (Shanghai Allan Biochemical science Co., Ltd.) were added to 25 mL of anhydrous tetrahydrofuran (national pharmaceutical chemical Co., Ltd.). The average molecular weight of the monomethoxypolyethylene glycol is 200 Da. The reaction mixture was reacted at 30 ℃ for 6 hours under stirring (200 rpm);
b3After the reaction is finished, the reaction mixture is subjected to vacuum distillation at 40 ℃ to remove anhydrous tetrahydrofuran, and the vacuum degree during vacuum distillation is controlled to be 1 Kpa;
c3Pouring the reaction mixture into precooled anhydrous ether, wherein the precooling temperature of the anhydrous ether is-5 ℃, carrying out reduced pressure suction filtration to collect precipitates, controlling the pressure of the reduced pressure suction filtration to be 1Kpa, and obtaining the precipitates as monomethoxy polyethylene glycol succinimide carbonate,
3. preparation of phenylalanine oligopeptide-monomethoxy polyethylene glycol copolymer
a1Adding 2g of phenylalanine oligopeptide, oxypolyethylene glycol succinimide carbonate and N, N-dimethylaniline (national drug group chemical reagent, Inc.) into 15 ml of anhydrous methanol (national drug group chemical reagent, Inc.), wherein the weight ratio of the phenylalanine oligopeptide, the oxypolyethylene glycol succinimide carbonate and the N, N-dimethylaniline is 1:1:8, and reacting the reaction mixture for 10 hours at 30 ℃ under the protection of nitrogen;
b1after the reaction is finished, adding 100ml of methanol precooled to 0 ℃, and carrying out suction filtration under reduced pressure, wherein the pressure range after pressure reduction is 10Kpa, and the suction filtration time is 0.5 hour;
c1Vacuum drying the filtrate at 30 deg.c for 10 hr, and adding acetone preheated to 40 deg.c until all the acetone is dissolved;
d1and standing the obtained solution at-5 ℃ overnight, and carrying out vacuum filtration for 0.5-1 hour under the reduced pressure of 5Kpa to obtain a precipitate of the phenylalanine oligopeptide-monomethoxy polyethylene glycol copolymer with the yield of 30%.
the substitution degree of the phenylalanine oligopeptide-monomethoxypolyethylene glycol copolymer is detected to be 1.01 by a 1HNMR means.
example 2
1. Protease catalyzed synthesis of phenylalanine oligopeptide
a21.5 g of L-phenylalanine methyl ester hydrochloride (national drug group chemical reagent Co., Ltd.), dimethyl sulfoxide (national drug group chemical reagent Co., Ltd.) and 3.5U of papain (Shanghai Wakai Biotech Co., Ltd.) were added to 2.5 mL of a disodium hydrogen phosphate-citric acid buffer solution (Nanchang rain dew laboratory Co., Ltd.) having a pH of 7.0 and 0.15M. The volume fraction of dimethyl sulfoxide was 30% phosphate buffer. The reaction mixture was placed in a constant temperature shaker (600rpm) and reacted at 30 ℃ for 8 hours;
b2After the reaction is finished, centrifuging the reaction mixture for 3 minutes at 10000 rpm to obtain a precipitate;
c2And washing the precipitate twice with deionized water and glacial ethanol respectively, and freeze-drying to obtain the product phenylalanine oligopeptide.
2. Preparation of Monomethoxypolyethylene glycol succinimide carbonate
a31.5 g of monomethoxypolyethylene glycol (Huaian petrochemical plant, Jiangsu province), 7 g of N, N' -disuccinimidyl carbonate (Huaian chemical reagent Co., Ltd.), and 5g of 4- (dimethylamino) pyridine (Shanghai Allan Biochemical science Co., Ltd.) were added to 25 mL of anhydrous tetrahydrofuran (Huaian chemical reagent Co., Ltd.). The average molecular weight of the monomethoxypolyethylene glycol is 400 Da. The reaction mixture was reacted at 30 ℃ for 6 hours under stirring (200 rpm);
b3After the reaction, the reaction mixture was vacuum distilled at 40 ℃ to remove the solvent (anhydrous tetrahydrofuran), and the degree of vacuum during vacuum distillation was controlled at 5 Kpa;
c3pouring the mixture into precooled anhydrous ether, wherein the precooling temperature of the anhydrous ether is-3 ℃, carrying out reduced pressure suction filtration to collect the precipitate, controlling the pressure of the reduced pressure suction filtration to be 5Kpa, and obtaining the precipitate as monomethoxy polyethylene glycol succinimide carbonate.
3. Preparation of phenylalanine oligopeptide-monomethoxy polyethylene glycol copolymer
a1Adding 3g of phenylalanine oligopeptide, oxypolyethylene glycol succinimide carbonate and N, N-dimethylaniline (national drug group chemical reagent, Inc.) into 20ml of anhydrous methanol (national drug group chemical reagent, Inc.), wherein the weight ratio of the phenylalanine oligopeptide, the oxypolyethylene glycol succinimide carbonate and the triethylamine is 1:1.5:10, and reacting the reaction mixture for 20 hours at 40 ℃ under the protection of nitrogen;
b1After the reaction is finished, adding 120ml of methanol precooled to-2 ℃, and carrying out suction filtration under reduced pressure, wherein the pressure range after pressure reduction is 1Kpa, and the suction filtration time is 0.5 hour;
c1vacuum drying the filtrate at 40 deg.C for 8 hr, adding acetone preheated to 46 deg.C until all the acetone is dissolved;
d1And standing the obtained solution at-2 ℃ overnight, and carrying out vacuum filtration, wherein the air pressure range after the pressure reduction is 1Kpa, the vacuum filtration time is 0.7 h, the obtained precipitate is the phenylalanine oligopeptide-monomethoxy polyethylene glycol copolymer, and the yield is 35%.
The substitution degree of the phenylalanine oligopeptide-monomethoxypolyethylene glycol copolymer is detected to be 1.05 by a 1HNMR means.
Example 3
1. protease catalyzed synthesis of phenylalanine oligopeptide
a21 g of L-phenylalanine methyl ester hydrochloride (national drug group chemical reagent Co., Ltd.) and dimethyl sulfoxide (national drug Collection) were added to 2.5 mL of 0.2M, pH 8.0.0 disodium hydrogen phosphate-citric acid buffer solution (manufactured by Nanchang Lu laboratory instruments Co., Ltd.)Cluster chemical reagents limited) and 5U alkaline protease (wokay biotechnology limited, shanghai) with a volume fraction of dimethyl sulfoxide being 50% phosphate buffer, placing the reaction mixture in a constant temperature oscillator (600rpm) and reacting at 70 ℃ for 10 hours;
b2After the reaction is finished, centrifuging the reaction mixture for 3 minutes at 10000 rpm to obtain a precipitate;
c2and washing the precipitate twice with deionized water and glacial ethanol respectively, and freeze-drying to obtain the product phenylalanine oligopeptide.
2. Preparation of Monomethoxypolyethylene glycol succinimide carbonate
a32g of monomethoxypolyethylene glycol (Huaian petrochemical plant, Jiangsu province), 8 g of N, N' -disuccinimidyl carbonate (Huaian chemical reagent Co., Ltd.), 6g of 4- (dimethylamino) pyridine (Shanghai Aradine Biotechnology Co., Ltd.) and the average molecular weight of the monomethoxypolyethylene glycol is 1000 Da were added to 25 mL of anhydrous tetrahydrofuran (Huaian chemical reagent Co., Ltd.). The reaction mixture was reacted at 30 ℃ for 6 hours under stirring (200 rpm);
b3after the reaction, the reaction mixture was vacuum distilled at 40 ℃ to remove the solvent (anhydrous tetrahydrofuran), and the degree of vacuum during vacuum distillation was controlled at 10 Kpa;
c3pouring the mixture into precooled anhydrous ether, wherein the precooling temperature of the anhydrous ether is 0 ℃, carrying out reduced pressure suction filtration to collect the precipitate, controlling the pressure of the reduced pressure suction filtration to be 10Kpa, and obtaining the precipitate as monomethoxy polyethylene glycol succinimide carbonate.
3. preparation of phenylalanine oligopeptide-monomethoxy polyethylene glycol copolymer
a15g of phenylalanine oligopeptide, oxypolyethylene glycol succinimide carbonate and N, N-dimethylaniline (national chemical Co., Ltd.) were added to 20ml of anhydrous methanol (national chemical Co., Ltd.). The weight ratio of the phenylalanine oligopeptide, the oxypolyethylene glycol succinimide carbonate and the N, N-dimethylaniline is 1: 2:12, and the reaction mixture is stirred under the protection of nitrogen,Reacting for 20 hours at 50 ℃;
b1after the reaction is finished, adding 150ml of methanol precooled to-5 ℃, and carrying out suction filtration under reduced pressure, wherein the pressure range after pressure reduction is 5Kpa, and the suction filtration time is 0.8 hour;
c1Vacuum drying the filtrate at 50 deg.C for 6 hr, adding acetone preheated to 52 deg.C until all the acetone is dissolved;
d1and standing the obtained solution at 0 ℃ overnight, and carrying out suction filtration under reduced pressure, wherein the air pressure range after the pressure reduction is 10Kpa, the suction filtration time is 0.5 hour, and the obtained precipitate is the phenylalanine oligopeptide-monomethoxy polyethylene glycol copolymer, and the yield is 45%.
The substitution degree of the phenylalanine oligopeptide-monomethoxypolyethylene glycol copolymer is 1.1 by detecting a 1HNMR means.
as can be seen from the above examples, the yield of the phenylalanine oligopeptide ~ monomethoxypolyethylene glycol copolymer is 30 ~ 45%, and the degree of substitution of the phenylalanine oligopeptide ~ monomethoxypolyethylene glycol copolymer detected by a 1HNMR method is 1.01 ~ 1.1.
Claims (9)
1. a method for preparing phenylalanine oligopeptide-monomethoxy polyethylene glycol copolymer by enzyme catalysis is characterized by comprising the following steps:
a1adding 2 ~ 5g of phenylalanine oligopeptide, oxy polyethylene glycol succinimide carbonate and N, N ~ dimethylaniline into 10 ~ 20ml of anhydrous methanol, wherein the weight ratio of the phenylalanine oligopeptide, the oxy polyethylene glycol succinimide carbonate and the N, N ~ dimethylaniline is 1 (1 ~ 2) to (8 ~ 12), and reacting the reaction mixture for 10 ~ 36 h at 30 ~ 50 ℃ under the protection of inert gas;
b1After the reaction is finished, adding 100ml to 150ml of methanol precooled to-5 ℃ to 0 ℃, and carrying out vacuum filtration under reduced pressure;
c1vacuum drying the filtrate at 30-50 deg.C for 6-10 hr, and adding acetone preheated to 40-52 deg.C until all the acetone is dissolved;
d1And standing the obtained solution at-5-0 ℃ overnight, and carrying out vacuum filtration to obtain a precipitate, namely the phenylalanine oligopeptide-monomethoxypolyethylene glycol copolymer.
2. The method for preparing the phenylalanine oligopeptide-monomethoxypolyethylene glycol copolymer under the enzyme catalysis of claim 1, which is characterized in that: step b1the range of the air pressure after decompression is 0 ~ 10Kpa, and the time of suction filtration is 0.5 ~ 1 hour.
3. The method for preparing the phenylalanine oligopeptide-monomethoxypolyethylene glycol copolymer under the enzyme catalysis of claim 1, which is characterized in that: step d1the range of the air pressure after decompression is 0 ~ 10Kpa, and the time of suction filtration is 0.5 ~ 1 hour.
4. The method for preparing the phenylalanine oligopeptide-monomethoxy polyethylene glycol copolymer under the enzyme catalysis of claim 1, wherein the synthetic method of the phenylalanine oligopeptide comprises the following steps:
a2adding 0.5 ~ 2.0 g of L ~ phenylalanine methyl ester hydrochloride, dimethyl sulfoxide and 2 ~ 5U protease into 2.0 ~ 3.0 mL of disodium hydrogen phosphate ~ citric acid buffer solution, placing a reaction mixture in a constant temperature oscillator, and reacting at 500 ~ 700 rpm and 30 ~ 70 ℃ for 5 ~ 10 hours, wherein the disodium hydrogen phosphate ~ citric acid buffer solution contains 0.1 ~ 0.2M of disodium hydrogen phosphate ~ citric acid, the pH value of the disodium hydrogen phosphate ~ citric acid buffer solution is 6.5 ~ 8.5, and the volume of the dimethyl sulfoxide is 20 ~ 50% of the volume of the phosphate buffer solution;
b2after the reaction is finished, centrifuging the reaction mixture for 2 ~ 4 minutes at 8000 ~ 12000rpm to obtain a precipitate;
c2and washing the precipitate with deionized water and glacial ethanol for 2 ~ 3 times respectively, and freeze ~ drying to obtain the phenylalanine oligopeptide.
5. The method for preparing the phenylalanine oligopeptide-monomethoxypolyethylene glycol copolymer under the enzyme catalysis of claim 4, which is characterized in that: step a2the protease is one of bromelain, papain, trypsin, neutral protease and alkaline protease.
6. The method for preparing phenylalanine oligopeptide-monomethoxypolyethylene glycol copolymer under enzyme catalysis as claimed in claim 1, wherein the preparation of methoxy polyethylene glycol succinimide carbonate adopts the following steps:
a3adding 1 ~ 2g of methoxypolyethylene glycol, 5 ~ 8 g of N, N' ~ disuccinimidyl carbonate and 3 ~ 6g of 4 ~ (dimethylamino) pyridine into 25 mL of anhydrous tetrahydrofuran, and reacting the reaction mixture at 25 ~ 35 ℃ for 4 ~ 8 hours under the condition of stirring at the speed of 150 ~ 250 rpm;
b3after the reaction is finished, carrying out vacuum distillation on the reaction mixture at the temperature of 35 ~ 45 ℃ to remove anhydrous tetrahydrofuran;
c3pouring the reaction mixture into precooled anhydrous ether, carrying out suction filtration under reduced pressure to collect precipitate, and obtaining the precipitate which is methoxy polyethylene glycol succinimide carbonate.
7. The method for preparing the phenylalanine oligopeptide-monomethoxypolyethylene glycol copolymer under the enzyme catalysis of claim 6, which is characterized in that: step a3the average molecular weight of the methoxypolyethylene glycol is 200 Da, 400 Da or 1000 Da.
8. the method for preparing the phenylalanine oligopeptide-monomethoxypolyethylene glycol copolymer under the enzyme catalysis of claim 6, which is characterized in that: step b3the vacuum degree during vacuum distillation is controlled ~ be 0 ~ 10 Kpa.
9. The method for preparing the phenylalanine oligopeptide-monomethoxypolyethylene glycol copolymer under the enzyme catalysis of claim 6, which is characterized in that: step c3in the method, the precooling temperature of the anhydrous ether is ~ 5 ~ 0 ℃, and the pressure of the reduced pressure suction filtration is controlled to be 0 ~ 10 Kpa.
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| CN112301081A (en) * | 2020-10-26 | 2021-02-02 | 江南大学 | Method for synthesizing phenylalanine oligopeptide-lysine oligopeptide copolymer through enzyme catalysis |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112301081A (en) * | 2020-10-26 | 2021-02-02 | 江南大学 | Method for synthesizing phenylalanine oligopeptide-lysine oligopeptide copolymer through enzyme catalysis |
| CN112301081B (en) * | 2020-10-26 | 2022-07-22 | 江南大学 | Method for synthesizing phenylalanine oligopeptide-lysine oligopeptide copolymer through enzyme catalysis |
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