CN110559931A - Method and device for pneumatically mixing samples - Google Patents
Method and device for pneumatically mixing samples Download PDFInfo
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- CN110559931A CN110559931A CN201910962337.3A CN201910962337A CN110559931A CN 110559931 A CN110559931 A CN 110559931A CN 201910962337 A CN201910962337 A CN 201910962337A CN 110559931 A CN110559931 A CN 110559931A
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- air
- sealing plug
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- 238000002156 mixing Methods 0.000 title claims abstract description 105
- 238000000034 method Methods 0.000 title claims abstract description 19
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 238000007789 sealing Methods 0.000 claims description 48
- 229920001971 elastomer Polymers 0.000 claims description 5
- 239000012780 transparent material Substances 0.000 claims description 3
- 238000000338 in vitro Methods 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 5
- 230000008859 change Effects 0.000 abstract description 4
- 238000003745 diagnosis Methods 0.000 abstract description 4
- 239000000203 mixture Substances 0.000 description 12
- 239000003153 chemical reaction reagent Substances 0.000 description 7
- 238000003756 stirring Methods 0.000 description 7
- 230000009471 action Effects 0.000 description 4
- 238000003825 pressing Methods 0.000 description 4
- 238000009434 installation Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 2
- FGRBYDKOBBBPOI-UHFFFAOYSA-N 10,10-dioxo-2-[4-(N-phenylanilino)phenyl]thioxanthen-9-one Chemical compound O=C1c2ccccc2S(=O)(=O)c2ccc(cc12)-c1ccc(cc1)N(c1ccccc1)c1ccccc1 FGRBYDKOBBBPOI-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/40—Mixers using gas or liquid agitation, e.g. with air supply tubes
- B01F33/401—Methods
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/40—Mixers using gas or liquid agitation, e.g. with air supply tubes
- B01F33/403—Mixers using gas or liquid agitation, e.g. with air supply tubes for mixing liquids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/40—Mixers using gas or liquid agitation, e.g. with air supply tubes
- B01F33/408—Controlling
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/40—Mixers using gas or liquid agitation, e.g. with air supply tubes
- B01F33/4094—Plants
Abstract
The invention relates to the technical field of in-vitro diagnosis, in particular to a method for pneumatically mixing samples; the adopted technical scheme is as follows: the method comprises the following steps: putting the sample into a closed container; inserting two air ducts into the container, and enabling an outlet of one air duct to be positioned above the liquid level of the sample and an outlet of the other duct to be positioned below the liquid level of the sample; and alternately supplying air to the two air guide tubes to uniformly mix the samples, wherein the frequency of the alternate air supply of the air guide tubes is 5-15 Hz. The invention can realize the uniform mixing of the sample by only controlling the flowing direction of the gas, and the change of the gas direction can be realized by the electromagnetic directional valve. Therefore, the invention can realize high-frequency rapid mixing and has high mixing efficiency; in the process of uniformly mixing, the sample is not in mechanical contact, and the components or the structure of the sample cannot be damaged; meanwhile, the invention can uniformly mix the samples which need to be stirred in a sealed environment.
Description
Technical Field
the invention relates to the technical field of in-vitro diagnosis, in particular to a method and a device for pneumatically mixing samples.
background
In the field of in vitro diagnostics, blending samples is an essential step in the sample processing process. At present, two main methods for stirring a sample to be detected in vitro diagnosis are provided: firstly, the sample and the sample reagent mixed liquid are mixed by a stirring rod manually, the sample and the sample reagent mixed liquid are difficult to be mixed fully by stirring the sample and the sample reagent mixed liquid by the stirring rod, the efficiency is low, and the labor is consumed; secondly, drive the stirring rake through electric actuator and stir, though can fully mix sample, sample and reagent mixed solution, nevertheless cause liquid to splash and destroy sample composition or sample structure easily.
disclosure of Invention
Aiming at the technical problems of low sample stirring and mixing efficiency, insufficient mixing and easy damage of samples in the existing in-vitro diagnosis, the invention provides a pneumatic sample mixing method, which can quickly and fully mix the samples without damaging the samples and can mix the samples in a closed container.
The invention is realized by the following technical scheme:
The method comprises the following steps:
putting the sample into a closed container;
inserting two air ducts into the container, and enabling an outlet of one air duct to be positioned above the liquid level of the sample and an outlet of the other duct to be positioned below the liquid level of the sample;
And alternately supplying air to the two air guide tubes to uniformly mix the samples, wherein the frequency of the alternate air supply of the air guide tubes is 5-15 Hz.
When the sample is uniformly mixed, the air guide pipe with the outlet positioned below the liquid level is emptied without applying pressure, and air with certain pressure is applied to the air guide pipe with the outlet positioned above the liquid level of the sample, so that the sample in the container enters the air guide pipe with the outlet positioned below the liquid level under the action of air pressure; then the gas guide pipe with the outlet positioned on the liquid level is emptied without applying pressure, and gas with certain pressure is applied to the gas guide pipe with the outlet positioned under the liquid level of the sample, so that the sample returns to the container under the action of air pressure; the two air ducts are supplied with air repeatedly and alternately, so that the purpose of pneumatically and uniformly mixing the samples is achieved.
the invention can realize the uniform mixing of the sample by only controlling the flowing direction of the gas, and the change of the gas direction can be realized by the electromagnetic directional valve. Therefore, the invention can realize high-frequency rapid mixing with high mixing efficiency, and the mixing frequency can reach more than 10 times/second. And in the process of mixing, the sample is not in mechanical contact, and the components or the structure of the sample cannot be damaged. Meanwhile, the invention can uniformly mix the samples which need to be stirred in a sealed environment.
The invention also provides a device for pneumatically mixing the samples, which is used for realizing the method and comprises the following steps:
The device comprises an open container, wherein a hollow blending body is arranged in the container, a gap is reserved between the blending body and the container, the upper end of the blending body is open, an air outlet is arranged at the lower part of the blending body, and the air outlet is positioned at the lower part of the container; the container mouth is adapted with a first sealing plug, the upper end of the mixing body is adapted with a second sealing plug, a gas passage is reserved between the first sealing plug and the second sealing plug, and the gas passage is communicated with the interior of the container. And (3) putting the sample into the container to enable the liquid level of the sample to be higher than the air outlet, and alternately supplying air to the cavity between the blending body and the second sealing plug, the interior of the container or the air channel to pneumatically blend the sample contained in the container.
Preferably, the volume of the upper part of the cavity of the mixing body is larger than that of the lower part of the cavity of the mixing body, so that the contact area between a sample entering the mixing body and the inner wall of the mixing body can be enlarged, and the sample can be further ensured to be fully mixed.
Preferably, the mixing body lower part is equipped with the recess, the gas outlet is located the recess tank bottom, reduces the impact of sample to the mixing body when flowing through the recess to ensure the integrality of sample.
Preferably, the recess is spoon form, the gas outlet is located recess tank bottom middle part to further reduce the sample when flowing to the impact of mixing the body.
Optionally, a first air duct is inserted into the first sealing plug, one end of the first air duct is located outside the container, and the other end of the first air duct is located in the air passage; and a second air duct is inserted into the second sealing plug, one end of the second air duct is positioned in the cavity of the uniformly mixing body, and the other end of the second air duct penetrates through the first sealing plug and is arranged outside the container.
Optionally, the first sealing plug and the second sealing plug are both made of rubber. The medical hollow needle head can conveniently penetrate through the sealing plug to supply air so as to supply air to the device when uniform mixing is needed; after the uniform mixing is finished, the needle head is pulled out to realize sealing so as to store the uniformly mixed sample in a sealing way.
Preferably, the container is made of transparent materials, so that the blending condition of the sample and the suction position of the needle can be observed conveniently.
Further, the container mouth is equipped with the container lid, the container lid is used for the shutoff container, first sealing plug is located container lid middle part, mixing body upper end and container lid fixed connection to the installation and the dismantlement of mixing body are convenient for.
Compared with the prior art, the invention has the following advantages and beneficial effects:
1. The invention can realize the uniform mixing of the sample by only controlling the flowing direction of the gas, and the change of the gas direction can be realized by the electromagnetic directional valve. Therefore, the invention can realize high-frequency rapid mixing with high mixing efficiency, and the mixing frequency can reach more than 10 times/second. And in the process of mixing, the sample is not in mechanical contact, and the components or the structure of the sample cannot be damaged. Meanwhile, the invention can uniformly mix the samples which need to be stirred in a sealed environment.
2. the volume of the upper part of the cavity of the mixing body is larger than that of the lower part, so that the contact area between a sample entering the mixing body and the inner wall of the mixing body can be enlarged, and the sample can be further ensured to be fully mixed.
3. The mixing body lower part is equipped with the recess of spoon form, the gas outlet is located recess tank bottom middle part, can reduce the sample to the impact of mixing body when flowing, can ensure that the composition or the structure of sample are not destroyed.
4. The first sealing plug and the second sealing plug are both made of rubber. The medical hollow needle head can conveniently penetrate through the sealing plug to supply air so as to supply air to the device when uniform mixing is needed; after the uniform mixing is finished, the needle head is pulled out to realize sealing so as to store the uniformly mixed sample in a sealing way.
Drawings
The accompanying drawings, which are included to provide a further understanding of the embodiments of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the principles of the invention. In the drawings:
FIG. 1 is a schematic structural diagram of the apparatus of the present invention.
Names of various parts in the drawings:
1-container, 2-blending body, 3-air outlet, 4-first sealing plug, 5-second sealing plug, 6-air channel, 7-groove, 8-first air duct, 9-second air duct and 10-container cover.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples and accompanying drawings, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not meant to limit the present invention.
Example 1
A method of pneumatically blending a sample, comprising the steps of:
Putting the obtained proper amount of sample into a closed container, and adding a reagent into the container if the reagent is required to be added;
Sealing the container, and inserting two air ducts into the container, so that the outlet of one air duct is positioned above the liquid level of the sample, and the outlet of the other duct is positioned below the liquid level of the sample;
the two air ducts are alternately supplied with air through an air source (such as an air pump) and reversing elements such as a reversing electromagnetic valve and the like so as to uniformly mix the sample.
Wherein, the frequency of air duct alternating air feed is 5 ~ 15Hz, and this embodiment is preferred 10 Hz.
when the sample is uniformly mixed, the air guide pipe with the outlet positioned below the liquid level is emptied without applying pressure, and air with certain pressure is applied to the air guide pipe with the outlet positioned above the liquid level of the sample, so that the sample in the container enters the air guide pipe with the outlet positioned below the liquid level under the action of air pressure; then the gas guide pipe with the outlet positioned on the liquid level is emptied without applying pressure, and gas with certain pressure is applied to the gas guide pipe with the outlet positioned under the liquid level of the sample, so that the sample returns to the container under the action of air pressure; the two air ducts are supplied with air repeatedly and alternately, so that the purpose of pneumatically and uniformly mixing the samples is achieved.
The invention can realize the uniform mixing of the sample by only controlling the flowing direction of the gas, and the change of the gas direction can be realized by the electromagnetic directional valve. Therefore, the invention can realize high-frequency rapid mixing with high mixing efficiency, and the mixing frequency can reach more than 10 times/second. And in the process of mixing, the sample is not in mechanical contact, and the components or the structure of the sample cannot be damaged. Meanwhile, the invention can uniformly mix the samples which need to be stirred in a sealed environment.
Example 2
A pneumatic sample blending device is used for realizing the method in the embodiment 1 and comprises an open container 1, wherein a hollow blending body 2 is arranged in the container 1. The blending body 2 can be supported and fixed in the container 1 through a fixing plate, the lower end of the blending body 2 can be directly fixedly connected with the inside of the container 1, and only a gap is required to be reserved between the blending body 2 and the container 1. The upper end of the mixing body 2 is opened, the lower part of the mixing body 2 is provided with an air outlet 3, and the air outlet 3 is positioned at the lower part of the container 1. The container 1 is provided with a first sealing plug 4 at the container opening, the upper end of the blending body 2 is provided with a second sealing plug 5, a gas passage 6 is reserved between the first sealing plug 4 and the second sealing plug 5, and the gas passage 6 is communicated with the inside of the container 1.
When the pneumatic mixing device is used, a sample is put into the container, so that the liquid level of the sample is higher than the gas outlet 3, and gas is alternately supplied to the cavity between the mixing body 2 and the second sealing plug 5, the interior of the container 1 or the gas channel 6, so that the sample in the container can be pneumatically mixed. The device is usually supplied with gas through a medical hollow needle through a sealing plug; the side wall of the container 1 can be provided with a port to supply gas to the inside of the container 1 or the gas channel 6, and a conduit is arranged in the container 1 to communicate the cavity of the blending body 2 with the outside of the container 1.
Preferably, the first sealing plug 4 and the second sealing plug 5 are made of rubber. The medical hollow needle head can conveniently penetrate through the sealing plug to supply air so as to supply air to the device when uniform mixing is needed; after the uniform mixing is finished, the needle head is pulled out to realize sealing so as to store the uniformly mixed sample in a sealing way.
Furthermore, the volume of the upper part of the cavity of the blending body 2 is larger than that of the lower part. Compared with a straight-through type uniform mixing body 2 cavity, under the same sample volume, the sample mixing device can increase the contact area between the sample and the side wall of the uniform mixing body 2 cavity so as to further mix the sample.
Preferably, the lower part of the blending body 2 is provided with a groove 7, and the air outlet 3 is positioned at the bottom of the groove 7. The impact of the sample on the mixing body 2 during flowing can be reduced through the groove 7, so that the integrity of the sample is ensured. More preferably, recess 7 is spoon form, gas outlet 3 is located recess 7 tank bottom middle part, 3 gas outlets promptly establish with the deepest of recess 7, can further reduce the sample to the even mixing body 2 impact.
Preferably, the container 1 is made of transparent material, such as polyurethane, organic glass, transparent PE, etc., so as to observe the blending condition of the sample and the drawing-in position of the needle.
preferably, the container 1 is provided with a container cover 10 at the container opening, the container cover 10 is used for sealing off the container 1, and the first sealing plug 4 is positioned in the middle of the container cover 10. Mixing body 2 upper end and container lid 10 fixed connection, consequently after sample and reagent add the completion, cover container lid 10 alright realize mixing body 2's installation, take off container lid 10 and can take out mixing body 2 together to mixing body 2's installation and dismantlement are convenient for.
Example 3
Based on embodiment 2, a first air duct 8 is inserted into the first sealing plug 4, one end of the first air duct 8 is located outside the container 1, and the other end of the first air duct 8 is located in the air passage 6. That is, the first gas-guide tube 8 communicates the inside of the container 1 with the outside of the container 1, so that gas can be introduced into the container. The interpolation of second sealing plug 5 is equipped with second air duct 9, 9 one end of second air duct is located the cavity of mixing body 2, the 9 other end of second air duct is equipped with outside container 1 through first sealing plug 4, and second air duct 9 communicates the inside cavity of mixing body 2 and the outside of container 1 promptly.
When the device is used, the air source is directly connected with the first air duct 8 and the second air duct 9, air is alternately supplied through the reversing valve, a needle head and the like do not need to be inserted, and pneumatic mixing operation is facilitated. Before or after the uniform mixing is completed, the first air duct 8 and the second air duct 9 are sealed by a rubber plug, a plug and the like on the application occasions where the samples need to be stored in a sealed mode.
The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are merely exemplary embodiments of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalent substitutions, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (10)
1. A method for pneumatically mixing a sample, comprising the steps of:
Putting the sample into a closed container;
Inserting two air ducts into the container, and enabling an outlet of one air duct to be positioned above the liquid level of the sample and an outlet of the other duct to be positioned below the liquid level of the sample;
and (4) alternately supplying air to the two air ducts to uniformly mix the samples.
2. the method for pneumatically mixing samples according to claim 1, wherein the alternating supply of air from the air ducts is at a frequency of 5 to 15 Hz.
3. A device for pneumatically mixing a sample for implementing the method according to claim 1 or 2, comprising an open container (1), characterized in that: a hollow blending body (2) is arranged in the container (1), a gap is reserved between the blending body (2) and the container (1), the upper end of the blending body (2) is open, a gas outlet (3) is arranged at the lower part of the blending body (2), and the gas outlet (3) is positioned at the lower part of the container (1); the container (1) container mouth is adapted with first sealing plug (4), mixing body (2) upper end is adapted with second sealing plug (5), leave gas passage (6) between first sealing plug (4), second sealing plug (5), gas passage (6) and container (1) inside intercommunication.
4. A device for pneumatically mixing samples according to claim 3, wherein: the volume of the upper part of the cavity of the uniform mixing body (2) is larger than that of the lower part.
5. A device for pneumatically mixing samples according to claim 3 or 4, wherein: the mixing body (2) lower part is equipped with recess (7), gas outlet (3) are located recess (7) tank bottom.
6. The apparatus of claim 5, wherein: the groove (7) is spoon-shaped, and the gas outlet (3) is positioned in the middle of the bottom of the groove (7).
7. A device for pneumatically mixing samples according to claim 3, wherein: a first air duct (8) is inserted into the first sealing plug (4), one end of the first air duct (8) is positioned outside the container (1), and the other end of the first air duct (8) is positioned in the gas channel (6); the second air duct (9) is inserted into the second sealing plug (5), one end of the second air duct (9) is located in the cavity of the uniformly mixing body (2), and the other end of the second air duct (9) penetrates through the first sealing plug (4) to be arranged outside the container (1).
8. A device for pneumatically mixing samples according to claim 3, wherein: the first sealing plug (4) and the second sealing plug (5) are both made of rubber.
9. a device for pneumatically mixing samples according to claim 8, wherein: the container (1) is made of transparent materials.
10. A device for pneumatically mixing samples according to claim 3, wherein: container (1) container mouth is equipped with container lid (10), container lid (10) are used for shutoff container (1), first sealing plug (4) are located container lid (10) middle part, mixing body (2) upper end and container lid (10) fixed connection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910962337.3A CN110559931A (en) | 2019-10-11 | 2019-10-11 | Method and device for pneumatically mixing samples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910962337.3A CN110559931A (en) | 2019-10-11 | 2019-10-11 | Method and device for pneumatically mixing samples |
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| Publication Number | Publication Date |
|---|---|
| CN110559931A true CN110559931A (en) | 2019-12-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910962337.3A Pending CN110559931A (en) | 2019-10-11 | 2019-10-11 | Method and device for pneumatically mixing samples |
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| CN (1) | CN110559931A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112795433A (en) * | 2021-03-19 | 2021-05-14 | 江西天佳生物工程股份有限公司 | Plant essential oil and separation and purification method thereof |
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| US20040019295A1 (en) * | 2002-07-25 | 2004-01-29 | Zhou David F. | Specimen collection and application apparatus |
| CN201607358U (en) * | 2009-12-11 | 2010-10-13 | 上海快德行生物技术有限公司 | Mixing device of medical testing sample |
| CN103439139A (en) * | 2013-09-13 | 2013-12-11 | 四川沃文特生物技术有限公司 | Disposable specimen collecting tube and applying method thereof |
| CN103439138A (en) * | 2013-09-13 | 2013-12-11 | 四川沃文特生物技术有限公司 | Liquid adding, even-mixing, sampling and pretreating integrated machine and realizing method thereof |
| CN210699886U (en) * | 2019-10-11 | 2020-06-09 | 四川沃文特生物技术有限公司 | Pneumatic device for mixing samples |
-
2019
- 2019-10-11 CN CN201910962337.3A patent/CN110559931A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09192475A (en) * | 1996-01-23 | 1997-07-29 | Terametsukusu Kk | Liquid pressurizing vessel |
| US20040019295A1 (en) * | 2002-07-25 | 2004-01-29 | Zhou David F. | Specimen collection and application apparatus |
| CN201607358U (en) * | 2009-12-11 | 2010-10-13 | 上海快德行生物技术有限公司 | Mixing device of medical testing sample |
| CN103439139A (en) * | 2013-09-13 | 2013-12-11 | 四川沃文特生物技术有限公司 | Disposable specimen collecting tube and applying method thereof |
| CN103439138A (en) * | 2013-09-13 | 2013-12-11 | 四川沃文特生物技术有限公司 | Liquid adding, even-mixing, sampling and pretreating integrated machine and realizing method thereof |
| CN210699886U (en) * | 2019-10-11 | 2020-06-09 | 四川沃文特生物技术有限公司 | Pneumatic device for mixing samples |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112795433A (en) * | 2021-03-19 | 2021-05-14 | 江西天佳生物工程股份有限公司 | Plant essential oil and separation and purification method thereof |
| CN112795433B (en) * | 2021-03-19 | 2021-07-27 | 江西天佳生物工程股份有限公司 | Mixing apparatus of plant essential oil |
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