CN110559234A

CN110559234A - Preparation method of liquorice extract and application of liquorice extract in freckle removing and whitening

Info

Publication number: CN110559234A
Application number: CN201910929199.9A
Authority: CN
Inventors: 李世�
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-09-29
Filing date: 2019-09-29
Publication date: 2019-12-13

Abstract

The invention discloses a preparation method of a liquorice extract and application of the liquorice extract in freckle removing and whitening, wherein the extraction and purification method comprises the following steps: (1) extraction of licorice residue: extracting licorice root residue with 95% ethanol to obtain crude extract of licorice root residue; (2) the liquorice residue enrichment rich in glabridin is prepared by a high-speed counter-current chromatography one-step method: the solvent system is dichloromethane, acetone, n-butanol and water (7:5:8:4, V/V), the upper phase is used as a mobile phase, the lower phase is used as a stationary phase, the crude extract of the licorice root residue is dissolved by mixed solvent of the upper phase and the lower phase which are mixed in an equal volume ratio to be used as a sample loading solution, the flow rate of the mobile phase is 3mL/min, the temperature is 25 ℃, and the rotating speed is 850r/min, and the licorice root residue enriched substance rich in glabridin is obtained. The method can prepare the licorice residue extract rich in glabridin from the licorice residue by a one-step method, wherein the content of the glabridin is more than 85 percent, and the extract can be used as a cosmetic raw material and a raw material for refining the glabridin.

Description

preparation method of liquorice extract and application of liquorice extract in freckle removing and whitening

Technical Field

The invention relates to glabridin and preparation and application of a liquorice extract rich in glabridin, in particular to an extraction and purification method of glabridin, a preparation method of the liquorice extract rich in glabridin and whitening application.

Background

With the improvement of science and technology and living standard, the demand of people for whitening cosmetics has been transformed from simple physical masking to functionality. The spot-removing/whitening products have a large share in our country and Asian markets. The whitening functional components are rich in natural components, the plant extracts account for 50 and 56.7 percent, and the main components are flavonoid compounds, such as glycyrrhiza glabra root extract, peony root extract, tea extract, arbutin and the like, and the whitening effect can be achieved by removing active oxygen and chelating metal ions to inhibit tyrosinase activity. In addition, the natural components include yeast extract and nidus Collocaliae extract. Some whitening ingredients such as pearl powder, hydrolyzed pearl, ferulic acid, extract of bird's nest, etc. are used only in domestic products.

according to investigation, the usage frequency of the nicotinamide in the freckle removing/whitening products is the highest, and the nicotinamide accounts for 31.4%; secondly, ascorbyl glucoside and arbutin account for 18.9 percent and 17.9 percent respectively. The use frequency of whitening functional components in imported products and domestic products is different. The whitening effective components in the first 10 bits of the use frequency in the imported product are ascorbyl glucoside, nicotinamide, arbutin, glycyrrhiza glabra root extract, 3-O-ethyl ascorbic acid, phenethyl resorcinol, scutellaria baicalensis root extract, peony root extract, potassium methoxysalicylate and magnesium ascorbyl phosphate; the whitening functional components in the first 10 bits of the use frequency in the domestic product are nicotinamide, arbutin, 3-O-ethyl ascorbic acid, ascorbic acid glucoside, sodium ascorbyl phosphate, glycyrrhiza glabra root extract, phenethyl resorcinol, magnesium ascorbyl phosphate, pearl powder and hydrolyzed pearl.

Among them, glabridin is widely used in whitening products at home and abroad due to its excellent whitening and freckle removing activity.

For a long time, the licorice residue after the licorice extract is extracted from licorice is discarded as waste residue. Research shows that the residual licorice slag still contains a large amount of glabridin, and the recovery and extraction of the glabridin are favorable for solving the problem of industrial production waste treatment, protecting and reasonably utilizing licorice plant resources and having good economic benefit and environmental benefit.

Chen military Ming et al published a paper "research on extraction process of glabridin in licorice residue" to comprehensively investigate the adsorption performance of various resins on glabridin, wherein Celite resin has high adsorption amount of glabridin in licorice residue, and is easy to elute, and the extraction purity is up to more than 50%. However, it depends on macroporous resin, and the recovery rate of macroporous resin column chromatography method is usually not very high, and the waste is serious (research on extraction process of glabridin from licorice residue, Xinjiang traditional Chinese medicine, vol. 32, No. 4 in 2014).

Wudiqiang et al published a paper "research on extraction process of glabridin in licorice residue", but the method is only a conventional solvent extraction method, and does not have subsequent concentration and enrichment steps, and high-purity glabridin cannot be obtained (research on extraction process of glabridin in licorice residue, science and technology forum).

the Wai sea published a study on the extraction process of glabridin from licorice residue, but the method is also a conventional solvent extraction method, and does not have subsequent concentration and enrichment steps, and high-purity glabridin cannot be obtained (the study on the extraction process of glabridin from licorice residue, science and technology forum).

The existing research shows that no mature and applicable process for preparing glabridin by utilizing licorice residue exists at present.

disclosure of Invention

The invention aims to overcome the defects of the prior art and provides a preparation method of a liquorice extract and application of the liquorice extract in freckle removing and whitening.

In order to achieve the purpose, the invention provides the following technical scheme:

A method for preparing a licorice extract comprises the following steps:

(1) Extraction of licorice residue

Crushing and sieving the dried licorice root residues, adding an ethanol water solution with the volume percentage concentration of 95%, and carrying out ultrasonic crushing extraction; and (4) carrying out suction filtration, and concentrating the filtrate to be dry to obtain a licorice residue crude extract.

(2) One-step method for preparing liquorice residue enrichment rich in glabridin by high-speed countercurrent chromatography

The solvent system is dichloromethane, acetone, n-butanol and water (7:5:8:4, V/V), the upper phase is used as a mobile phase, and the lower phase is used as a stationary phase; dissolving the crude extract of licorice residue with mixed solvent of upper phase and lower phase in equal volume ratio to obtain sample solution; the flow rate of the mobile phase is 3mL/min, the temperature is 25 ℃, and the rotating speed is 850 r/min; the specific operation method comprises the following steps: pumping the stationary phase into an HSCCC spiral pipe at the flow rate of 30mL/min, opening a chromatograph, adjusting the rotating speed of the spiral pipe, adjusting the elution mode to be reverse-connection forward rotation, rotating speed of 850r/min, pumping the mobile phase into the spiral pipe at the flow rate of 3mL/min after the rotating speed is stable, continuing pumping the mobile phase into the spiral pipe after the mobile phase flows out of the spiral pipe, balancing for 30min, injecting the sample solution into the spiral pipe through a sample injection valve, starting timing, collecting 120-150 min effluent liquid, and concentrating until the effluent liquid is dry to obtain the licorice extract rich in glabridin.

Preferably, step (1) is carried out by sieving with a 40-mesh sieve after pulverization.

Preferably, the feed-liquid ratio in the step (1) is 1g to 20 mL.

preferably, the parameters of the ultrasonic disruption extraction in the step (1) are as follows: frequency of 30kHz, ultrasonic power of 400W, and extraction time of 60 min.

Preferably, the loading concentration of step (2) is 20 mg/mL.

Preferably, the loading volume of step (2) is 10 mL.

The extract prepared by the preparation method is used for removing freckles and whitening.

The extract prepared by the above preparation method can be used for preparing glabridin.

The beneficial effects are that:

The method can prepare the licorice residue extract rich in glabridin from the licorice residue by a one-step method, wherein the content of the glabridin is more than 85 percent, and the extract can be used as a cosmetic raw material and a raw material for refining the glabridin.

Drawings

FIG. 1 is a high performance liquid chromatogram of glabridin standard, licorice residue concentrate, and glabridin;

FIG. 2 shows the inhibitory activity of the licorice root residue concentrate prepared in example 1 on tyrosinase;

FIG. 3 is a graph showing the inhibitory activity of glabridin on tyrosinase, prepared in example 1.

Detailed Description

Example 1: preparation of glabridin extract, recrystallization and purification

First, experimental material

The licorice residue is prepared by self-making, namely waste (prepared by moistening and slicing Glycyrrhiza glabra Linne, adding water and decocting for three times, 2 hours each time) after the Glycyrrhiza glabra Linne extract is extracted and produced is dried for later use.

Glabridin standard, Chengdu prilisi Biotech limited.

HSCCC-300C high-speed countercurrent chromatography, a TBP-5002 constant-pressure constant-flow pump, a DC-0506 low-temperature constant-temperature tank, a TBD-2000 ultraviolet detector, Shanghai Hotan Biotechnology GmbH.

Ethanol and other chemical reagents, analytically pure, Nanjing chemical reagents GmbH.

Second, Experimental methods

1. Extraction of licorice residue

Crushing the dried licorice root residues, sieving the crushed licorice root residues with a 40-mesh sieve, adding an ethanol water solution with the volume percentage concentration of 95% according to the material-liquid ratio of 1:20(g/mL), and carrying out ultrasonic crushing extraction with the parameters as follows: extracting for 60min at the frequency of 30kHz and the ultrasonic power of 400W; and (4) carrying out suction filtration, and concentrating the filtrate to be dry to obtain a licorice residue crude extract.

2. One-step method for preparing licorice root residue enrichment rich in glabridin by high-speed countercurrent chromatography (HSCCC)

the HSCCC solvent system comprises dichloromethane, acetone, n-butanol and water (7:5:8:4, V/V), wherein the upper phase is used as a mobile phase, and the lower phase is used as a stationary phase. Dissolving the crude extract of radix Glycyrrhizae residue with mixed solvent of upper phase and lower phase at equal volume ratio to obtain loading solution. The flow rate of the mobile phase is 3mL/min, the temperature is 25 ℃, the rotating speed is 850r/min, the sample loading quantity is 10mL, and the sample loading mass concentration is 20 mg/mL. The specific operation method comprises the following steps: pumping the stationary phase into an HSCCC spiral pipe at the flow rate of 30mL/min, opening a chromatograph, adjusting the rotating speed of the spiral pipe, adjusting the elution mode to be reverse-connection forward rotation, rotating speed of 850r/min, pumping the mobile phase into the spiral pipe at the flow rate of 3mL/min after the rotating speed is stable, continuing pumping the mobile phase into the spiral pipe after the mobile phase flows out of the spiral pipe, balancing for 30min, injecting 10mL of 20mg/mL sample solution into the spiral pipe through a sample injection valve, starting timing, collecting 120-150 min effluent liquid, and concentrating until the effluent liquid is dry to obtain the licorice residue enriched substance rich in glabridin.

3. Preparation of glabridin by recrystallization

dissolving 100mg of the licorice residue enrichment in 5mL of 1, 2-propylene glycol, adding 20mL of water, uniformly oscillating, standing at 4 ℃ for 12h, collecting precipitate, washing with acetone precooled at 4 ℃, recrystallizing for 1 time, washing with acetone, and drying with nitrogen to obtain glabridin. ESI-MS determination of molecular formula C₂₀H₂₀O₄. The HPLC chromatographic retention time is consistent with that of the glabridin standard.

4. High performance liquid chromatography for determining purity of radix Glycyrrhizae residue concentrate and glabridin

Gradient elution was performed using an Agilent ZORBAX extended-C18 (250 mm. times.4.6 mm, 5 μm) column with a mobile phase composition and elution gradient: the phase A is acetonitrile, the phase B is water, the concentration of A is 50-70% in 0-5 min, the concentration of A is 70-100% in 5-25 min, the concentration of A is 100% in 25-40 min, the flow rate is 1.0mL/min, the column temperature is 40 ℃, the detection wavelength is 280nm, and the sample injection amount is 10 mu L.

Dissolving glabridin standard, licorice residue concentrate and glabridin with acetonitrile, preparing standard solution and test solution of 0.8mg/mL concentration, and injecting the standard solution and test solution into liquid chromatograph for analysis according to the chromatographic parameters.

Third, experimental results

According to calculation, 4.5g of licorice residue enrichment can be prepared from 1kg of dried licorice residue.

According to calculation, 792mg of glabridin can be prepared from 1g of licorice residue enrichment.

HPLC chromatograms of glabridin standard, licorice residue concentrate, and glabridin are shown in FIG. 1, HPLC normalized purity of glabridin in licorice residue concentrate is 87.8%, and HPLC normalized purity of glabridin is 98.4%.

therefore, the HSCCC can be used for preparing the licorice residue enrichment rich in glabridin from the crude extract of the licorice residue by a one-step method, wherein the content of the glabridin is more than 85 percent, and the licorice residue enrichment can be used as a cosmetic raw material and a raw material for refining the glabridin. Furthermore, the recrystallization method can refine the glabridin with the purity of more than 98 percent from the liquorice residue enrichment, has simple steps, does not need column chromatography, and is suitable for industrialization.

Example 2: speckle removing and whitening activity of licorice residue enrichment

Solution preparation:

L-tyrosine solution: dissolving L-tyrosine in phosphate buffer (pH 6.8) to give a solution with a concentration of 0.4 mg/mL;

Tyrosinase solution: dissolving and diluting tyrosinase with phosphate buffer (pH 6.8) to obtain 100U/mL solution;

Liquid to be detected: the licorice root residue concentrate prepared in example 1 was dissolved and diluted in a phosphate buffer (pH 6.8) to prepare solutions having concentrations of 0.2, 0.4, 0.6, 0.8, and 1.0 mg/mL.

Reaction system and reaction parameters:

a reaction system is constructed according to the composition in the table 1, 1.0mL of L-tyrosine solution is accurately transferred into a 10mL test tube, then the solution to be tested is added, the temperature is preserved for 20min in water bath at 30 ℃, 1.0mL of tyrosinase solution is accurately transferred, the mixture is uniformly mixed, the reaction is carried out for 10min at 30 ℃, a blank control is replaced by phosphate buffer (pH is 6.8), and the experiment is repeated for 3 times. After the reaction, the reaction solution was quickly transferred to a quartz cuvette and the absorbance of the solution was measured at 475 nm.

TABLE 1 reaction System composition

the inhibition rate I of the liquid to be detected on the activity of the tyrosinase is calculated according to the following formula.

in the formula, A1 is the light absorption value of the reaction system when the liquid to be detected is not added and tyrosinase is added; a2 is the light absorption value of the reaction system without the liquid to be detected and tyrosinase; a3 is the light absorption value of the reaction system when the liquid to be detected and tyrosinase are added; a4 is the light absorption value of the reaction system when the liquid to be detected is added but no tyrosinase is added.

The inhibition rate curves of the licorice root residue enrichments with different concentrations on tyrosinase activity are shown in fig. 2, and it can be seen that the licorice root residue enrichments have significant inhibition effect on tyrosinase and show concentration dependence.

Example 3: spot-removing and whitening activity of glabridin

Solution preparation:

Liquid to be detected: the glabridin prepared in example 1 was dissolved and diluted in a phosphate buffer (pH 6.8) to prepare solutions having concentrations of 0.1, 0.3, 0.5, 0.7, and 0.9 mg/mL.

Reaction system and reaction parameters:

A reaction system is constructed according to the composition shown in Table 2, 1.0mL of L-tyrosine solution is accurately transferred into a 10mL test tube, then the solution to be tested is added, the temperature is preserved for 20min in water bath at 30 ℃, 1.0mL of tyrosinase solution is accurately transferred, the mixture is uniformly mixed, the reaction is carried out for 10min at 30 ℃, a blank control is replaced by phosphate buffer (pH is 6.8), and the experiment is repeated for 3 times. After the reaction, the reaction solution was quickly transferred to a quartz cuvette and the absorbance of the solution was measured at 475 nm.

TABLE 2 reaction System composition

The inhibition rate curves of different concentrations of glabridin on tyrosinase activity are shown in fig. 3, and it can be seen that glabridin has significant inhibition effect on tyrosinase and is concentration-dependent.

Claims

1. The preparation method of the licorice extract is characterized by comprising the following steps:

(1) Extraction of licorice residue

2. The method of claim 1, wherein: and (2) crushing the mixture in the step (1) and then sieving the crushed mixture with a 40-mesh sieve.

3. The method of claim 1, wherein: the ratio of the materials to the liquid in the step (1) is 1g to 20 mL.

4. the method according to claim 1, wherein the parameters of the ultrasonic disruption extraction in the step (1) are: frequency of 30kHz, ultrasonic power of 400W, and extraction time of 60 min.

5. the method of claim 1, wherein: the sample concentration in the step (2) is 20 mg/mL.

6. The method of claim 1, wherein: the sample volume in step (2) was 10 mL.

7. Use of the extract prepared by the preparation method of any one of claims 1 to 6 for removing freckles and whitening skin.

8. Use of the extract prepared by the preparation method of any one of claims 1 to 6 for preparing glabridin.