CN110554114B - Method for analyzing oligomeric isomaltose and isomers thereof in yoghourt - Google Patents
Method for analyzing oligomeric isomaltose and isomers thereof in yoghourt Download PDFInfo
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Abstract
The invention relates to an analysis method of isomaltose hypgather and isomers thereof in yoghourt, which adopts an ultra-high performance liquid chromatography-ion mobility-triple quadrupole mass spectrometry tandem flight time mass spectrometry technology and HMSEThe method comprises the steps of collecting a sample in a mode, quantifying by using primary and secondary ion fragments, performing qualitative determination by using retention time and ion mobility drift time, accurately analyzing maltose, maltotriose, isomaltose, panose and isomaltotriose in the yoghourt in multiple dimensions, establishing an UPLC-IMS-QTof method for oligomerizing isomaltooligosaccharide in the yoghourt, filling the blank in the field, providing a more efficient and accurate analysis method for workers in the industry, and providing technical support for detecting the oligosaccharide content of the yoghourt in the market.
Description
Technical Field
The invention relates to the field of chemical analysis, and in particular relates to a method for measuring the content of isomaltooligosaccharide and isomers thereof.
Background
Isomaltose hypgather (IMO) refers to a kind of oligosaccharide formed by combining two or more glucose with alpha-1, 6-glycosidic bond, and the main common food is: isomaltose (IG 2), Panose (Panose, P), Isomaltotriose (IG 3), etc. are called isomaltooligosaccharides for distinguishing them from maltose due to their different molecular conformations. The isomaltose hypgather has excellent heat resistance and acid resistance, the syrup with the concentration of 50 percent can not be differentiated even being heated for a long time at the PH3 and the temperature of 120 ℃, the water activity AW of the isomaltose hypgather at 75 percent and the temperature of 25 ℃ is 0.75 which is close to cane sugar, and common bacteria, yeast and mould can not grow in the environment that AW is less than or equal to 0.8, so the isomaltose hypgather has excellent preservative effect; in addition, isomaltooligosaccharide is added into bread and fermented milk (yogurt) and is not used by yeast and lactobacillus and remains in food to exert its functional property and promote the development of probiotics. The intestinal probiotics can convert oligosaccharides into exercise fatty acid, so that the intestinal tract is in a weakly acidic environment, the propagation of most harmful bacteria is inhibited, and the propagation of probiotics such as bifidobacteria and lactobacillus is promoted. Many countries have established standards for inclusion of oligosaccharides in food accessories, rather than as food additives.
The yoghurt is a fermented food for many people in China, contains oligosaccharides mainly including Fructo-oligosaccharides (FOS) and Galacto-oligosaccharides (GOS), and IMO can supplement nutrition better than FOS and GOS. Because FOS is unstable under acidic conditions and is easily decomposed in the fermentation process, FOS is not easy to enter the intestinal tract to take effect; while GOS can be utilized and absorbed by the human body, most asians cause intestinal discomfort, flatulence and diarrhea with high doses of GOS, so IMO is an excellent functional oligosaccharide in fermented milk. Studies have shown that modern people have a tendency to consume low cholesterol, low fat and functional foods. In order to increase the product sales volume, many merchants can add isomaltose hypgather as a marketing strategy. At present, an HPLC-RID method is used as a standard method for detecting SN/T3637-2013, but defects that panose and isomaltotriose cannot be effectively distinguished, the detection limit is high and the like exist, and qualitative and quantitative analysis for more effectively separating isomaltooligosaccharide is always a challenge for food analysis workers.
The ultra-high performance liquid phase can enable the substances to be separated more quickly, the drift time of the substances with different molecular configurations can be obtained by the ion mobility meter, and the parameter has one-dimensional identification of the isomers of the isomaltooligosaccharide more than that of the traditional liquid chromatography, so that the isomaltooligosaccharide can be further separated and analyzed better. At present, no research on detecting isomaltooligosaccharide in food by ultra-high performance liquid phase-ion mobility-quadrupole series flight time mass spectrometry exists. The UPLC-IMS-QTof method for the oligomeric isomaltose in the yogurt is established by taking a fermented dairy product with a complex matrix as a research object, fills the blank of the field, provides a more efficient and accurate analysis method for workers in the industry and provides technical support for detecting the oligosaccharide content of the yogurt in the market.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides an analysis method of isomaltose hypgather and isomers thereof in yoghourt, which can be used for accurately separating and analyzing the isomaltose hypgather and the isomers thereof from a plurality of latitudes so as to provide a technical means for detecting trace isomaltose hypgather in the yoghourt.
The technical scheme provided by the invention is as follows:
the method for analyzing and identifying isomaltose hypgather and isomers thereof in the yogurt comprises the steps of detecting the yogurt by using ultra-high performance liquid chromatography-ion mobility-triple quadrupole mass spectrometry tandem flight time mass spectrometry, carrying out quantification by monitoring primary and secondary ion fragments, carrying out qualitative determination by monitoring retention time and ion mobility drift time, and judging the content of isomaltose hypgather and isomers thereof in the yogurt.
In one embodiment, the above method comprises the steps of:
s1, preparing a standard solution: respectively taking standard substances of maltose, isomaltose, maltotriose, panose and isomaltotriose, and preparing a series of mixed standard solutions with a concentration;
s2, pretreatment of a yoghourt sample: taking 1-5g of yogurt sample, adding 5-15mL of precipitator, shaking uniformly, adjusting pH to be neutral, centrifuging at 0-10 ℃, filtering, and collecting filtrate to be tested;
S3, detection and analysis: respectively injecting a series of mixed standard working solutions in the S1 and a solution to be detected of a sample in the S2 on an ultra performance liquid chromatography-ion mobility-triple quadrupole mass spectrometry tandem flight time mass spectrum, drawing a working curve by taking the concentration as a horizontal coordinate X and the peak area as a vertical coordinate Y, and calculating the concentration of maltose, isomaltose, maltotriose, panose and isomaltotriose in the sample in the step S2 through the working curve.
In one embodiment, the precipitant is an aqueous ethanol solution in a ratio of 9:1 in step S2.
In one embodiment, the detection conditions for ultra high performance liquid chromatography are as follows:
and (3) chromatographic column: 1.7 μm, 2.1X 50mm ACQUITY UPLC BEH Amide amino column;
a mobile phase A: ammonia solution;
mobile phase B: acetonitrile;
column flow rate: 0.3 mL/min;
sample introduction amount: 1 mu L of the solution;
gradient elution procedure: 0-6min, mobile phase A22%, mobile phase B78%; 6-8min, mobile phase A28%, mobile phase B72%; 35% of mobile phase A and 65% of mobile phase B in 8-12.5 min; 12.5-15min mobile phase A22% and mobile phase B78%.
In one embodiment, the detection conditions for the ion mobility-triple quadrupole mass spectrometry tandem time-of-flight mass spectrometry are as follows:
An ion source: a negative chemical source;
ion source voltage: -2500V;
an ionization mode: electrospray ionization;
ion mobility mode: a high-resolution collision area mode;
ion source temperature: 120 ℃;
desolvation temperature: 250 ℃;
desolvation gas flow rate: 800L/h;
the detection mode is as follows: high resolution ion mobility full scan mode.
In one embodiment, the monitoring conditions for ion mobility-triple quadrupole tandem time of flight mass spectrometry are as follows:
maltose: retention time 2.45s, parent ion m/z341.109, daughter ion m/z283.265, ion drift time 8.9ms, collision voltage-6V;
isomaltose: retention time 3.25s, parent ion m/z341.107, daughter ion m/z283.259, ion drift time 8.5s, collision voltage-6 v;
maltotriose: retention time 7.43s, parent ion m/z503.165, daughter ion m/z405.101, ion drift time 7.8s, collision voltage-6 v;
panose: retention time 8.25s, parent ion m/z503.163, daughter ion m/z383.119, ion drift time 7.5s, collision voltage-6 v;
isomaltotriose: retention time 9.3s, parent ion m/z503.165, daughter ion m/z383.121, ion drift time 7.3s, collision voltage-6 v.
The beneficial effects of the above scheme are as follows:
1. The method for detecting the content of the oligomeric isomaltose and the isomers thereof in the yogurt by applying the ultra-high performance liquid chromatography-ion mobility-triple quadrupole mass spectrometry tandem flight time mass spectrometry technology is established, the ionic mobility is used for accurately determining the isomers of the oligosaccharides in the yogurt for the first time, primary and secondary ion fragments are used for quantification from multi-dimensional analysis, retention time and ion mobility drift time are used for determining the isomers, and the problem that the oligomeric disaccharide and trisaccharide equivalent isomers are difficult to accurately distinguish due to similarity of the ion fragments is solved.
2. The method provided by the scheme is efficient, accurate, strong in selectivity and high in sensitivity, the recovery rate of the oligosaccharide target compound reaches 75.2% -111.2%, the relative standard deviation in the day is between 1.8% -6.1%, the relative standard deviation in the day is between 3.6% -7.2%, and the relative standard deviation is lower than 10%, and the method has good reproducibility. The detection limit of the method reaches 0.112-0.259mg/kg, and the target compound presents an obvious linear relation in the concentration range of 0.1-100mg/L, so that an efficient and reliable technical means is provided for detecting isomaltooligosaccharide and isomers thereof in the yogurt.
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FIG. 1 shows a sample and a sample labeled extracted ion spectrum according to an embodiment of the present invention.
Detailed description of the preferred embodiments
The present invention will be further described with reference to the following examples for facilitating understanding of those skilled in the art, and the contents of the examples are not intended to limit the present invention.
A method for analyzing oligomeric isomaltose and isomers thereof in yoghourt comprises the following steps:
s1, preparing a standard solution: accurately weighing maltose, maltotriose, isomaltose, panose and isomaltotriose standard substances, each 10.00mg of the standard substances, respectively dissolving the standard substances in a 10mL volumetric flask with ultrapure water, and fixing the volume to 10mL to prepare 5 standard single-standard stock solutions of 1000 mg/L; then respectively taking 0.1mL of each 1000mg/L standard single-standard stock solution, fixing the volume to 10mL in a 10mL capacity bottle by using ultrapure water to obtain 10mg/L standard mixed standard stock solution of 5 kinds of oligosaccharides, and placing the standard mixed standard stock solution in a refrigerator at 4 ℃ for storage; diluting the stock solution with ultrapure water step by step to prepare standard series solutions with concentrations of 0.1mg/L, 0.2mg/L, 0.5mg/L, 1mg/L, 2mg/L, 5mg/L and 10mg/L, storing in a refrigerator at 4 ℃ and testing for use.
S2, pretreatment of a yoghourt sample: accurately weighing 1g of yogurt sample into a 50mL centrifuge tube, adding 1mL of ultrapure water for dissolving and fully oscillating, then using 9mL of absolute ethyl alcohol for constant volume to 10mL, carrying out vortex oscillation, adjusting the pH to be neutral by using ammonia water, carrying out ultrasonic treatment for 20min, centrifuging for 5min at 8000r/min and 4 ℃, filtering through a 0.22 mu m filter membrane, collecting filtrate into a sample injection bottle, and waiting for detection on a computer.
S3, detection and analysis: respectively injecting a series of mixed standard working solutions in S1 and a solution to be detected of a sample in S2 on an ultra performance liquid chromatography-ion mobility-triple quadrupole mass spectrometry tandem flight time mass spectrum, drawing a working curve by taking the concentration as a horizontal coordinate X and the peak area as a vertical coordinate Y, and calculating the concentration of maltose, isomaltose, maltotriose, panose and isomaltotriose in the S2 sample through the working curve.
Specifically, the detection conditions of the ultra-high performance liquid chromatography are as follows:
a chromatographic column: 1.7 μm, 2.1X 50mm ACQUITY UPLC BEH Amide amino column;
mobile phase A: 0.1% ammonia solution;
mobile phase B: acetonitrile (chromatographically pure);
column flow rate: 0.3 mL/min;
sample introduction amount: 1 mu L of the solution;
gradient elution program changes are shown in table 1;
TABLE 1 UPLC gradient elution procedure
Specifically, the detection conditions of the ion mobility-triple quadrupole mass spectrometry tandem flight time mass spectrometry are as follows:
an ion source: a negative chemical source;
ion source voltage: -2500V;
an ionization mode: electrospray ionization;
ion mobility mode: a high-resolution collision area mode;
ion source temperature: 120 ℃;
desolvation temperature: 250 ℃;
desolvation gas flow rate: 800L/h;
the detection mode is as follows: a high-resolution ion mobility full scan mode;
The monitoring conditions are shown in table 2;
TABLE 25 monitoring conditions for sugars
The result of the detection
As shown in fig. 1, it can be seen that the drift time of different ions in Ion Mobility (IMS) can accurately identify isomers of different oligosaccharides from complex peak patterns, which is an effect that is difficult to achieve by conventional ion pair and retention time analysis methods, and the detection results of the samples are shown in table 3.
TABLE 3 results of sample testing
In order to verify the feasibility and the accuracy of the method, 1g of a yoghurt sample is taken and respectively added with 5 oligosaccharide standard intermediate solutions of 2mg/L, 5mg/L and 10mg/L, 6 times of repeated experiments are respectively carried out on each concentration, the experiment of the standard recovery rate is carried out according to the method, and the related results are shown in tables 4.1-4.3.
TABLE 4.1 recovery of oligosaccharide in yogurt samples normalized (2mg/L)
TABLE 4.2 recovery of oligosaccharide in yogurt samples at 5mg/L
TABLE 4.3 Targeted (10mg/L) recovery of oligosaccharides in the yogurt samples
TABLE 5 method detection limits
It can be seen that the normalized recovery rate of the oligosaccharide target compound in the present disclosure reaches 75.2% -111.2%, the relative standard deviation in the day is between 1.8% -6.1%, the relative standard deviation in the day is between 3.6% -7.2%, and both are lower than 10%, which indicates better reproducibility; and the detection limit of the method reaches 0.112-0.259mg/kg, and the method is suitable for trace analysis of the content of the oligosaccharide in the yoghourt.
In order to further verify the feasibility of the method, 8 different brands of yogurt on the market were continuously extracted to detect the oligosaccharide content, and the relevant detection results are shown in table 6.
TABLE 6 oligosaccharide results table for yogurt samples of different brands
From table 4, it can be seen that all the eight detected yoghurts contain maltose and isomaltose, and some of the eight detected yoghurts contain three isomaltooligosaccharides, namely maltotriose, panose and isomaltotriose, so that the method proves that the target analyte of the sample can be effectively separated and accurately determined by the ultra high performance liquid chromatography tandem mass spectrometry, and the method is suitable for determination of a large number of different samples.
The above-described embodiments are preferred implementations of the invention, and the invention may be embodied in other forms without departing from the spirit or essential attributes thereof.
Claims (2)
1. The method for analyzing the isomaltose hypgather and the isomers thereof in the yogurt is characterized in that the yogurt is detected by using ultra-high performance liquid chromatography-ion mobility-triple quadrupole mass spectrometry tandem flight time mass spectrometry, quantification is carried out by monitoring primary and secondary ion fragments, qualitative determination is carried out by monitoring retention time and ion mobility drift time, and the content of the isomaltose hypgather and the isomers thereof in the yogurt is judged; the method comprises the following steps:
S1, preparing a standard solution: preparing a series of mixed standard solutions with a concentration by respectively taking standard substances of maltose, isomaltose, maltotriose, panose and isomaltotriose;
s2, pretreatment of a yoghourt sample: taking 1-5g of yogurt sample, adding 5-15mL of precipitator, shaking uniformly, adjusting pH to be neutral, centrifuging at 0-10 ℃, filtering, and collecting filtrate to be tested;
s3, detection and analysis: respectively injecting a series of mixed standard working solutions in S1 and a sample solution to be detected in S2 on an ultra performance liquid chromatography-ion mobility-triple quadrupole mass spectrometry tandem flight time mass spectrum, drawing a working curve by taking the concentration as a horizontal coordinate X and the peak area as a vertical coordinate Y, and calculating through the working curve to obtain the concentrations of maltose, isomaltose, maltotriose, panose and isomaltotriose in the sample in the step S2;
the monitoring conditions of the ion mobility-triple quadrupole mass spectrometry tandem flight time mass spectrometry are as follows:
maltose: retention time 2.45s, parent ion m/z341.109, daughter ion m/z283.265, ion drift time 8.9ms, collision voltage-6 v;
isomaltose: retention time 3.25s, parent ion m/z341.107, daughter ion m/z283.259, ion drift time 8.5ms, collision voltage-6 v;
Maltotriose: retention time 7.43s, parent ion m/z503.165, daughter ion m/z405.101, ion drift time 7.8ms, and collision voltage-6 v;
panose: retention time 8.25s, parent ion m/z503.163, daughter ion m/z383.119, ion drift time 7.5s, collision voltage-6 v;
isomaltotriose: retention time 9.3s, parent ion m/z503.165, daughter ion m/z383.121, ion drift time 7.3ms, collision voltage-6 v;
in step S2, the precipitant is an ethanol aqueous solution with a ratio of 9: 1;
the detection conditions of the ultra high performance liquid chromatography of the step S3 are as follows:
a chromatographic column: 1.7 μm, 2.1X 50mm ACQUITY UPLC BEH Amide amino column;
mobile phase A: 0.1% ammonia solution;
mobile phase B: chromatographically pure acetonitrile;
column flow rate: 0.3 mL/min;
sample introduction amount: 1 mu L of the solution;
the gradient elution procedure was as follows:
2. the method of claim 1, wherein the ion mobility-triple quadrupole mass spectrometry tandem time-of-flight mass spectrometry is detected under the following conditions:
an ion source: a negative chemical source;
ion source voltage: -2500V;
an ionization mode: electrospray ionization;
ion mobility mode: a high-resolution collision area mode;
ion source temperature: 120 ℃;
desolvation temperature: 250 ℃;
Desolvation gas flow rate: 800L/h;
the detection mode is as follows: high resolution ion mobility full scan mode.
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| CN111610275A (en) * | 2020-06-08 | 2020-09-01 | 山东省分析测试中心 | Method for determining malto-oligosaccharide and application of method in field of product quality control |
| CN111812231B (en) * | 2020-06-29 | 2022-11-11 | 广东省生物工程研究所（广州甘蔗糖业研究所） | Method for separating and detecting sucrose/isomaltulose in food |
| CN112461913B (en) * | 2020-11-06 | 2022-02-22 | 华南农业大学 | Method for improving identification capability of isomer compound |
| CN116559337B (en) * | 2023-07-11 | 2023-09-12 | 中国医学科学院北京协和医院 | Method for simultaneously and quantitatively analyzing glucose tetramer and maltotetraose |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105699478A (en) * | 2016-03-21 | 2016-06-22 | 长春中医药大学 | Method for quickly identifying sugar |
| CN108169385A (en) * | 2018-03-12 | 2018-06-15 | 安徽古井贡酒股份有限公司 | A kind of method using six kinds of glucides in ultra performance liquid chromatography concatenation QDa simultaneously quick detection health liquor |
| CN108426965A (en) * | 2018-06-06 | 2018-08-21 | 胡贝贞 | The detection method of isomaltose, Isomaltotriose, maltose, panose in yellow rice wine |
-
2019
- 2019-09-13 CN CN201910867591.5A patent/CN110554114B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105699478A (en) * | 2016-03-21 | 2016-06-22 | 长春中医药大学 | Method for quickly identifying sugar |
| CN108169385A (en) * | 2018-03-12 | 2018-06-15 | 安徽古井贡酒股份有限公司 | A kind of method using six kinds of glucides in ultra performance liquid chromatography concatenation QDa simultaneously quick detection health liquor |
| CN108426965A (en) * | 2018-06-06 | 2018-08-21 | 胡贝贞 | The detection method of isomaltose, Isomaltotriose, maltose, panose in yellow rice wine |
Non-Patent Citations (4)
| Title |
|---|
| Separation of isomeric disaccharides by traveling wave ion mobility mass spectrometry using CO2 as drift gas;Fasciotti, M等;《JOURNAL OF MASS SPECTROMETRY》;20121231;第47卷(第12期);摘要 * |
| 超高效液相色谱-串联质谱法测定卷烟中果糖、葡萄糖、蔗糖、麦芽糖;蒋佳芮等;《化学分析计量》;20190331;第28卷(第2期);22-25 * |
| 食品中低聚异麦芽糖高效液相色谱检测方法研究;傅博强等;《食品工业科技》;20101031;第31卷(第10期);388-391，395 * |
| 饮料中异麦芽低聚糖的测定;陈桂茹等;《中国食品卫生杂志》;20020930;第14卷(第05期);14-16 * |
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Address after: No.10, shiliugang Road, Haizhu District, Guangzhou City, Guangdong Province 510000 Patentee after: Institute of bioengineering, Guangdong Academy of Sciences Address before: No.10, shiliugang Road, Haizhu District, Guangzhou City, Guangdong Province 510000 Patentee before: GUANGDONG PROVINCIAL BIOENGINEERING INSTITUTE (GUANGZHOU SUGARCANE INDUSTRY RESEARCH INSTITUTE) Address after: No.10, shiliugang Road, Haizhu District, Guangzhou City, Guangdong Province 510000 Patentee after: Institute of biological and medical engineering, Guangdong Academy of Sciences Address before: No.10, shiliugang Road, Haizhu District, Guangzhou City, Guangdong Province 510000 Patentee before: Institute of bioengineering, Guangdong Academy of Sciences |