CN110550745A - Water purifying agent capable of inhibiting vibrio parahaemolyticus - Google Patents
Water purifying agent capable of inhibiting vibrio parahaemolyticus Download PDFInfo
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- CN110550745A CN110550745A CN201910906865.7A CN201910906865A CN110550745A CN 110550745 A CN110550745 A CN 110550745A CN 201910906865 A CN201910906865 A CN 201910906865A CN 110550745 A CN110550745 A CN 110550745A
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- bacillus amyloliquefaciens
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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Abstract
The invention provides a water purifying agent capable of inhibiting vibrio parahaemolyticus, which comprises bacillus amyloliquefaciens culture solution, bacillus amyloliquefaciens bacteriostatic extract and nano-scale humus. The nano-scale humus adopted by the invention has large specific surface area and a plurality of functional groups, is rich in a plurality of trace elements, and can well activate and promote the fixation and growth of bacillus on the nano-scale humus to generate a large amount of antibacterial active substances. During the growth and reproduction process of the bacillus, a large amount of pollutants such as nitrogen, phosphorus, organic matters and the like in the aquaculture water can be absorbed, and the aquaculture water is purified. The bacillus bacteriostatic extract has stronger inhibition capability on vibrio parahaemolyticus than the bacillus culture solution, can help bacillus amyloliquefaciens to gain advantages in competitive growth with vibrio parahaemolyticus, and can obviously inhibit the growth of vibrio parahaemolyticus.
Description
Technical Field
The invention relates to the field of aquaculture, and in particular relates to a water purifying agent containing bacillus amyloliquefaciens and capable of inhibiting vibrio parahaemolyticus.
Background
Vibrio parahaemolyticus is considered to be the main cause of prawn early death syndrome and hepatopancreatic necrosis syndrome. The farmers mainly use chemical disinfectants, antibiotics and probiotics to inhibit the growth of vibrio parahaemolyticus. The chemical disinfectant can stimulate the prawns, and the chemical agent residue can cause secondary pollution. The use of antibiotics is easy to generate drug resistance and drug residue, which are harmful to human health. The vibrio parahaemolyticus can rapidly propagate in large quantities by utilizing nutrients in water, and the existing probiotics are difficult to form competitive advantages in the real use process and cannot effectively inhibit the growth of the vibrio parahaemolyticus.
Therefore, how the probiotics compete with vibrio parahaemolyticus in the culture water body for growth and inhibiting vibrio parahaemolyticus become the problem to be solved in the field.
Disclosure of Invention
The first purpose of the invention is to provide a water purifying agent capable of inhibiting vibrio parahaemolyticus, which comprises a bacillus amyloliquefaciens culture solution, a bacillus amyloliquefaciens bacteriostatic extract and nano-scale humus.
Preferably, the volume ratio of the bacillus amyloliquefaciens culture solution to the bacillus amyloliquefaciens bacteriostatic extract is as follows: the culture solution of the bacillus amyloliquefaciens is 15-20: 1-3 of a bacillus amyloliquefaciens bacteriostatic extract.
Preferably, the volume ratio of the bacillus amyloliquefaciens culture solution to the bacillus amyloliquefaciens bacteriostatic extract is as follows: and (3) preparing a bacillus amyloliquefaciens culture solution, namely 19:1 of a bacillus amyloliquefaciens bacteriostatic extract.
Preferably, the mass concentration of the nano-scale humus in the water purifying agent is 10-25%.
Preferably, the mass concentration of the nano-scale humus in the water purifying agent is 20%.
Preferably, the Bacillus amyloliquefaciens is a Bacillus amyloliquefaciens screened by the invention, and the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) strain is named as LBJK08 and is preserved in Guangdong province microorganism strain preservation center in 2019, 6 and 13 months, wherein the preservation address is Guangdong, and the preservation number is as follows: GDMCC No. 60690.
The second purpose of the invention is to provide a preparation method of a water purifying agent capable of inhibiting vibrio parahaemolyticus, which comprises the following steps: firstly, mixing a bacillus amyloliquefaciens culture solution and nano-scale humus, and carrying out aeration culture to ensure that the bacillus amyloliquefaciens culture solution is attached to the nano-scale humus to grow; then adding the bacillus amyloliquefaciens bacteriostatic extract, and uniformly mixing to obtain the water purifying agent capable of inhibiting the vibrio parahaemolyticus.
Preferably, the Bacillus amyloliquefaciens is a Bacillus amyloliquefaciens screened by the invention, and the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) strain is named as LBJK08 and is preserved in Guangdong province microorganism strain preservation center in 2019, 6 and 13 months, wherein the preservation address is Guangdong, and the preservation number is as follows: GDMCC No. 60690.
Preferably, the bacillus amyloliquefaciens bacteriostatic extract is prepared by the following method: adding HCl into the supernatant of the culture solution of the bacillus amyloliquefaciens to obtain a precipitate, dissolving the precipitate in methanol, adding NaOH, stirring and centrifuging, and filtering the obtained supernatant to obtain a filtrate, namely the bacillus amyloliquefaciens LBJK08 antibacterial extract.
Preferably, the nanoscale humus is prepared by the following method: taking a humus raw material, carrying out primary treatment of crushing and drying, crushing the humus to below 16 meshes and the water content of the humus to be lower than 10%, and grinding the humus to particles with the particle size of less than 0.1 micron to obtain the nanoscale humus.
The invention has the beneficial effects that: the nano-scale humus adopted by the invention has large specific surface area and a plurality of functional groups, is rich in a plurality of trace elements, and can well activate and promote the fixation and growth of bacillus on the nano-scale humus to generate a large amount of antibacterial active substances. During the growth and reproduction process of the bacillus, a large amount of pollutants such as nitrogen, phosphorus, organic matters and the like in the aquaculture water can be absorbed, and the aquaculture water is purified. The bacillus bacteriostatic extract has stronger inhibition capability on vibrio parahaemolyticus than the bacillus culture solution, can help bacillus amyloliquefaciens to gain advantages in competitive growth with vibrio parahaemolyticus, and can obviously inhibit the growth of vibrio parahaemolyticus.
Drawings
FIG. 1 is a graph showing the bacteriostatic effect of a Bacillus amyloliquefaciens water purifying agent (wherein 1 represents the bacteriostatic zone of the Bacillus amyloliquefaciens water purifying agent; 2 represents the bacteriostatic zone of ampicillin; 3 represents the culture solution of Bacillus amyloliquefaciens; and 4 represents the bacteriostatic zone of gentamicin).
Detailed Description
In order to more concisely and clearly demonstrate technical solutions, objects and advantages of the present invention, the following detailed description of the present invention is provided with reference to specific embodiments and accompanying drawings.
Example 1
This example provides a method for preparing a water purifying agent for inhibiting Vibrio parahaemolyticus
1. Preparation of Bacillus amyloliquefaciens LBJK08 culture solution
A Bacillus amyloliquefaciens strain LBJK08 stored at-20 ℃ is inoculated in LB culture medium (one of common culture media) with the salinity of twenty thousand by 1 percent of inoculation amount and cultured for 24 hours at the conditions of 30 ℃ and 150 rpm. Inoculating the activated culture solution into the culture medium with 1% inoculum size, and culturing at 30 deg.C and 150rpm for 24 hr to obtain Bacillus amyloliquefaciens LBJK08 culture solution.
2. preparation of bacteriostatic extract of Bacillus amyloliquefaciens LBJK08
Bacillus amyloliquefaciens LBJK08 stored at-20 ℃ is inoculated into LB culture medium with the inoculation amount of 1 percent and cultured for 24h under the conditions of 30 ℃ and 150 rpm. The bacterial solution obtained by the activation culture was inoculated again in LB medium at an inoculum size of 1% and cultured at 30 ℃ for 24 hours at 150 rpm. The cultured bacterial liquid is subpackaged in a 100mL centrifuge tube, the bacterial cell is removed by centrifugation at 10000rpm for 16min at 4 ℃, the pH of the supernatant is adjusted to 2.0 by 1M HCl solution, and the supernatant is kept overnight at 4 ℃. And then centrifuging at 4 ℃ and 10000rpm for 16min, taking the precipitate, dissolving the precipitate obtained by centrifuging every 50mL of bacterial liquid with 1mL of methanol (HPLC chromatogram pure), adjusting the pH to 7.0 with 1M of MaOH, stirring at low temperature for 5h, then centrifuging at 4 ℃ and 10000rpm for 16min, filtering the obtained supernatant with a 0.22 mu M vacuum filter, and obtaining the filtrate which is the antibacterial extract of the bacillus amyloliquefaciens LBJK 08.
3. Preparation of nanoscale humus
Taking a humus raw material (purchased from Jinan Dongyiwei industrialism chemical Co., Ltd.), putting into a raw material processor for crushing and primary drying treatment, so that the humus is crushed to below 16 meshes and the water content is lower than 10%. And (3) transferring the primarily treated humus raw material into a high-speed controllable orbital transfer multistage ball grinder, and grinding to a nano level (the grain size is less than 0.1 micron) to obtain the nano-level humus.
4. Preparation of water purifying agent capable of inhibiting vibrio parahaemolyticus
Firstly, 950ml of bacillus amyloliquefaciens LBJK08 culture solution is mixed with 200 g of nano-scale humus, aeration culture is carried out for half an hour to activate the bacillus, the bacillus is attached to the nano-scale humus to propagate and grow, then 50ml of bacteriostatic extract is added, and the mixture is uniformly mixed to prepare the water purifying agent capable of inhibiting vibrio parahaemolyticus.
Example 2
The inhibition effect of the water purifying agent for inhibiting vibrio parahaemolyticus prepared in example 1 on vibrio parahaemolyticus was verified by zone of inhibition experiments, and the results are shown in fig. 1. As can be seen from FIG. 1, the diameter of the inhibition zone of the water purifying agent is 20.00 mm, while the diameter of the inhibition zone of the Bacillus amyloliquefaciens LBJK08 culture solution is 14.00 mm, and the diameters of the inhibition zones of ampicillin and gentamicin are 17.43 mm and 18.08 mm respectively. Therefore, the water purifying agent has the strongest activity of inhibiting vibrio parahaemolyticus, the second time of ampicillin and gentamicin, and the weakest inhibition effect of the bacillus amyloliquefaciens LBJK08 culture solution. The water purifying agent of the invention can replace antibiotics and can be used as a substitute of antibiotics for inhibiting vibrio parahaemolyticus.
Example 3
The only difference between this example and example 1 is that: the volume ratio of the bacillus amyloliquefaciens culture solution to the bacillus amyloliquefaciens bacteriostatic extract is as follows: and (2) the bacillus amyloliquefaciens culture solution is a bacillus amyloliquefaciens bacteriostatic extract 15:1, and the mass concentration of the nano-scale humus in the water purifying agent is 25%.
Example 4
the only difference between this example and example 1 is that: the volume ratio of the bacillus amyloliquefaciens culture solution to the bacillus amyloliquefaciens bacteriostatic extract is as follows: and the bacillus amyloliquefaciens culture solution is a bacillus amyloliquefaciens bacteriostatic extract which is 16:1, and the mass concentration of the nano-scale humus in the water purifying agent is 25%.
Example 5
The only difference between this example and example 1 is that: the volume ratio of the bacillus amyloliquefaciens culture solution to the bacillus amyloliquefaciens bacteriostatic extract is as follows: and (2) a bacillus amyloliquefaciens culture solution, namely a bacillus amyloliquefaciens bacteriostatic extract, wherein the mass concentration of the nano-scale humus in the water purifying agent is 20%.
Example 6
The only difference between this example and example 1 is that: the volume ratio of the bacillus amyloliquefaciens culture solution to the bacillus amyloliquefaciens bacteriostatic extract is as follows: and (3) the bacillus amyloliquefaciens culture solution is a bacillus amyloliquefaciens bacteriostatic extract which is 18:2, and the mass concentration of the nano-scale humus in the water purifying agent is 22%.
Example 7
The only difference between this example and example 1 is that: the volume ratio of the bacillus amyloliquefaciens culture solution to the bacillus amyloliquefaciens bacteriostatic extract is as follows: and (2) the bacillus amyloliquefaciens culture solution is a bacillus amyloliquefaciens bacteriostatic extract which is 20:3, and the mass concentration of the nano-scale humus in the water purifying agent is 10%.
Bacteriostatic circle experiments were carried out on the water purifying agents prepared in examples 1 and 3 to 6, and the results are shown in table 1:
Table 1:
| Sample (I) | Diameter of bacteriostatic circle (mm) |
| Example 1 | 20.00 |
| Example 3 | 18.22 |
| Example 4 | 17.11 |
| Example 5 | 18.65 |
| Example 6 | 18.68 |
| Example 7 | 19.22 |
As can be seen from table 1, the bacteriostatic effect of example 1 is the best, and the bacteriostatic activity of examples 3 to 7 is not enhanced with the increase of the content of the single component, so it can be inferred that the compatibility of the components in the invention has a certain synergistic effect, and the bacteriostatic effect is not a simple increase of the function of the single component.
Example 8
The implementation provides a using method of the water purifying agent capable of inhibiting vibrio parahaemolyticus
The water purifying agent capable of inhibiting vibrio parahaemolyticus is sprinkled into a culture pond to be treated according to 2 liters per mu.
Example 9 Bacillus amyloliquefaciens LBJK08 species screening and identification
Adopting 2216E culture medium to separate several different strains from Yangjiang shrimp pond bottom water sample, and using a point seeding method to screen and separate the strain with strongest vibrio parahaemolyticus antagonistic ability, which comprises the following steps:
Culturing the bottom water sample with 2216E at 30 deg.C under 150rpm for 24 hr, diluting with 0.85% sterilized normal saline, spreading on 2216E agar medium, and culturing at 30 deg.C for 48 hr. Individual colonies were scraped with a one-time inoculating loop and spotted separately on 2216E agar medium previously coated with a pathogen indicator (cultured in 3% sodium chloride alkali peptone water medium for 24h) and each experimental colony was numbered separately. And (3) observing and measuring the inhibition zone after culturing at constant temperature of 30 ℃ for 24h, and finally screening out a strain with the strongest vibrio parahaemolyticus antagonistic capability, wherein the strain is named as LBJK 08.
Morphological characteristics of strain LBJK 08:
Colony characteristics: on 2216E agar medium, the colony is flat, round, neat in edge, milky white and opaque.
Cell characteristics: gram-positive, short rod-shaped, capable of forming spore, 0.6-0.7 μm X0.8, 0.8-1.0 μm in size.
Physiological and biochemical characteristics: see table 2.
Table 2: physiological and biochemical characteristics of bacillus amyloliquefaciens
And (3) molecular identification:
After repeated cultivation and screening for many times, transferring the screened and separated antagonistic bacterial strain with stable antagonistic action to a biological engineering (Shanghai) limited company for 16S rDNA sequencing, submitting the obtained sequence to NCBI for Blast homology comparison, and finally reaching 99% homology with Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), wherein the gene sequence is shown as SEQ ID NO: 1 is shown.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
SEQUENCE LISTING
<110> institute of petroleum and fine chemical engineering, Guangdong province
<120> a water purifying agent for inhibiting Vibrio parahaemolyticus
<130> 9.23
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1376
<212> DNA
<213> bacterium
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cgcatggttc agacataaaa ggtggcttcg gctaccactt acagatggac ccgcggcgca 180
ttagctagtt ggtgaggtaa cggctcacca aggcgacgat gcgtagccga cctgagaggg 240
tgatcggcca cactgggact gagacacggc ccagactcct acgggaggca gcagtaggga 300
atcttccgca atggacgaaa gtctgacgga gcaacgccgc gtgagtgatg aaggttttcg 360
gatcgtaaag ctctgttgtt agggaagaac aagtgccgtt caaatagggc ggcaccttga 420
cggtacctaa ccagaaagcc acggctaact acgtgccagc agccgcggta atacgtaggt 480
ggcaagcgtt gtccggaatt attgggcgta aagggctcgc aggcggtttc ttaagtctga 540
tgtgaaagcc cccggctcaa ccggggaggg tcattggaaa ctggggaact tgagtgcaga 600
agaggagagt ggaattccac gtgtagcggt gaaatgcgta gagatgtgga ggaacaccag 660
tggcgaaggc gactctctgg tctgtaactg acgctgagga gcgaaagcgt ggggagcgaa 720
caggattaga taccctggta gtccacgccg taaacgatga gtgctaagtg ttagggggtt 780
tccgcccctt agtgctgcag ctaacgcatt aagcactccg cctggggagt acggtcgcaa 840
gactgaaact caaaggaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt 900
cgaagcaacg cgaagaacct taccaggtct tgacatcctc tgacaatcct agagatagga 960
cgtccccttc gggggcagag tgacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag 1020
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ggcactctaa ggtgactgcc ggtgacaaac cggaggaagg tggggatgac gtcaaatcat 1140
catgcccctt atgacctggg ctacacacgt gctacaatgg acagaacaaa gggcagcgaa 1200
accgcgaggt taagccaatc ccacaaatct gttctcagtt cggatcgcag tctgcaactc 1260
gactgcgtga agctggaatc gctagtaatc gcggatcagc atgccgcggt gaatacgttc 1320
ccgggccttg tacacaccgc ccgtcacacc acgagagttt gtaacacccg aagtcg 1376
Claims (10)
1. a water purifying agent capable of inhibiting vibrio parahaemolyticus is characterized by comprising a bacillus amyloliquefaciens culture solution, a bacillus amyloliquefaciens bacteriostatic extract and nano-scale humus.
2. The water purifying agent for inhibiting vibrio parahaemolyticus of claim 1, wherein the volume ratio of the bacillus amyloliquefaciens culture solution to the bacillus amyloliquefaciens bacteriostatic extract is as follows: the culture solution of the bacillus amyloliquefaciens is 15-20: 1-3 of a bacillus amyloliquefaciens bacteriostatic extract.
3. The water purifying agent for inhibiting vibrio parahaemolyticus of claim 2, wherein the volume ratio of the bacillus amyloliquefaciens culture solution to the bacillus amyloliquefaciens bacteriostatic extract is as follows: and (3) preparing a bacillus amyloliquefaciens culture solution, namely 19:1 of a bacillus amyloliquefaciens bacteriostatic extract.
4. The water purifying agent for inhibiting vibrio parahaemolyticus according to claim 1, wherein the mass concentration of the nano-scale humus in the water purifying agent is preferably 10-25%.
5. The water purifying agent for inhibiting vibrio parahaemolyticus according to claim 4, wherein the mass concentration of the nano-scale humic substance in the water purifying agent is preferably 20%.
6. the water purifying agent for inhibiting vibrio parahaemolyticus according to any one of claims 1 to 4, wherein the bacillus amyloliquefaciens is a bacillus amyloliquefaciens strain screened by the invention, and the bacillus amyloliquefaciens (bacillus amyloliquefaciens) strain is named as LBJK08 and is preserved in Guangdong province microorganism strain collection center in 2019, 6 and 13 days, and the preservation number is as follows: GDMCC No. 60690.
7. A preparation method of a water purifying agent capable of inhibiting vibrio parahaemolyticus is characterized by comprising the following steps: firstly, mixing a bacillus amyloliquefaciens culture solution and nano-scale humus, and carrying out aeration culture to ensure that the bacillus amyloliquefaciens culture solution is attached to the nano-scale humus to grow; then adding the bacillus amyloliquefaciens bacteriostatic extract, and uniformly mixing to obtain the water purifying agent capable of inhibiting the vibrio parahaemolyticus.
8. The method for preparing a water purifying agent capable of inhibiting vibrio parahaemolyticus according to claim 7, wherein the bacillus amyloliquefaciens is a bacillus amyloliquefaciens strain screened by the invention, the bacillus amyloliquefaciens (bacillus amyloliquefaciens) strain is named as LBJK08, and is preserved in Guangdong province microorganism strain collection center in 2019, 6 and 13 days, and the preservation number is as follows: GDMCC No. 60690.
9. The method for preparing the water purifying agent capable of inhibiting vibrio parahaemolyticus of claim 7, wherein the bacillus amyloliquefaciens bacteriostatic extract is prepared by the following method: adding HCl into the supernatant of the culture solution of the bacillus amyloliquefaciens to obtain a precipitate, dissolving the precipitate in methanol, adding NaOH, stirring and centrifuging, and filtering the obtained supernatant to obtain a filtrate, namely the bacillus amyloliquefaciens LBJK08 antibacterial extract.
10. The method for preparing a water purifying agent for inhibiting vibrio parahaemolyticus according to claim 7, wherein the nanoscale humus is prepared by the following method: taking a humus raw material, carrying out primary treatment of crushing and drying, crushing the humus to below 16 meshes and the water content of the humus to be lower than 10%, and grinding the humus to particles with the particle size of less than 0.1 micron to obtain the nanoscale humus.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103045498A (en) * | 2012-10-10 | 2013-04-17 | 浙江大学 | Bacillus amyloliquefaciens and application thereof |
| CN103966118A (en) * | 2013-12-20 | 2014-08-06 | 中国水产科学研究院黄海水产研究所 | Bacillusamyloliquefaciens and application thereof |
| CN104195067A (en) * | 2014-07-07 | 2014-12-10 | 中国水产科学研究院南海水产研究所 | Bacillus amyloliquefaciens and application thereof in aquaculture |
| CN104974950A (en) * | 2015-05-11 | 2015-10-14 | 山东大学（威海） | Marine Bacillus amyloliquefaciens and uses thereof |
| KR20170050298A (en) * | 2015-10-30 | 2017-05-11 | 금오공과대학교 산학협력단 | Composition using microorgarnisms with water purification function |
-
2019
- 2019-09-24 CN CN201910906865.7A patent/CN110550745A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103045498A (en) * | 2012-10-10 | 2013-04-17 | 浙江大学 | Bacillus amyloliquefaciens and application thereof |
| CN103966118A (en) * | 2013-12-20 | 2014-08-06 | 中国水产科学研究院黄海水产研究所 | Bacillusamyloliquefaciens and application thereof |
| CN104195067A (en) * | 2014-07-07 | 2014-12-10 | 中国水产科学研究院南海水产研究所 | Bacillus amyloliquefaciens and application thereof in aquaculture |
| CN104974950A (en) * | 2015-05-11 | 2015-10-14 | 山东大学（威海） | Marine Bacillus amyloliquefaciens and uses thereof |
| KR20170050298A (en) * | 2015-10-30 | 2017-05-11 | 금오공과대학교 산학협력단 | Composition using microorgarnisms with water purification function |
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