CN110550732A

CN110550732A - Biological antidote and application thereof

Info

Publication number: CN110550732A
Application number: CN201910903921.1A
Authority: CN
Inventors: 苟晓蕾
Original assignee: Pro Biotechnology Shanghai Co Ltd
Current assignee: Pro Biotechnology Shanghai Co Ltd
Priority date: 2019-09-24
Filing date: 2019-09-24
Publication date: 2019-12-10

Abstract

the invention relates to a biological antidote and application thereof, wherein the biological antidote comprises the following components in percentage by mass: 1-10% of organic composite acid, 3-6% of carbohydrate, 0.5-1% of algae extract, 0.2-0.5% of enzyme, 0.5-1% of surfactant, 0.06-0.12% of trace elements and 100% of water; the organic composite acid is extracted from weathered lignite; the organic composite acid contains phenolic acid with the molecular weight less than or equal to 450; the biological antidote is used for wastewater treatment. The biological antidote provided by the invention can improve the oxygen consumption rate of microorganisms, improve the capacity of degrading pollutants by activated sludge, has simple production process and low operation cost, and can be widely applied to industrial wastewater treatment biochemical systems.

Description

Biological antidote and application thereof

Technical Field

the invention relates to the technical field of wastewater treatment, in particular to a biological antidote and application thereof.

Background

The waste water treatment can be divided into municipal sewage and industrial sewage according to the water quality source, some industrial sewage have complex components and poor biodegradability, and some substances have toxicity, such as heavy metals, sulfides, cyanides, nitriles, phenols and the like. During the treatment of the industrial wastewater, the concentration of toxic substances is strictly controlled under normal conditions, but the phenomenon that the toxic substances inhibit the propagation of bacteria is caused when the upstream production is abnormal or the water quality fluctuates greatly during the major overhaul, and finally pollutants such as Chemical Oxygen Demand (COD) and ammonia nitrogen in effluent cannot meet the requirement of environmental protection. In the prior art, various detoxification methods are provided, including using biological antidotes, but the detoxification effect is often not thorough, the effluent COD value still needs to be further reduced, and the treatment method is complex.

CN105236591B discloses a biological antidote. The biological antidote is prepared from the following raw materials in percentage by mass: 8-10% of hydrolyzed casein, 1.5-2.0% of EDTA, 2.0-. 5% of VC, 61.0-2.0% of VbB, 1.5-2.0% of biotin, 2.0-2.5% of ferrous ammonium sulfate, 0.5-0.8% of protease, 0.5-0.8% of cellulase, 0.5-0.8% of amylase and the balance of distilled water. The antidote can be used for treating water quality fluctuation and detoxifying substances with toxicity to activated sludge, and can be used for relieving the impact of the water quality fluctuation on a biochemical system to the maximum extent, but the COD (chemical oxygen demand) of the effluent of the biochemical section treated by the antidote is still higher than 200mg/L, and the treatment effect needs to be further improved.

CN101423283B discloses a method and a device for efficiently detoxifying organic wastewater, the method mainly comprises three steps of mixing, catalytic reaction, oxidant reduction and the like, the organic wastewater to be treated is introduced into a pipeline mixer to be fully mixed with H ₂ O ₂ and O ₃, then the organic wastewater enters a catalytic reactor consisting of a catalytic reaction unit, is subjected to catalytic reaction under the induction of ultraviolet light (UV) and the action of a catalyst taking active carbon as a carrier, then enters an oxidant reduction unit, and is subjected to reduction reaction after being added with a reducing agent, so that wastewater meeting the requirements is led out.

Therefore, the research on a novel biological antidote is urgently needed in the field, the oxygen consumption rate of microorganisms is improved, the pollutant degradation capacity of activated sludge is improved, the production process is simple, and the running cost is low.

disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a biological antidote which can improve the oxygen consumption rate of microorganisms and the pollutant degradation capacity of activated sludge, has simple production process and low operation cost, and can be widely applied to an industrial wastewater treatment biochemical system.

In order to achieve the purpose, the invention adopts the following technical scheme:

The invention provides a biological antidote, which comprises the following components in percentage by mass:

The organic composite acid is extracted from weathered lignite;

the organic complex acid contains phenolic acid with molecular weight less than or equal to 450, such as 98, 178, 196, 218, 292, 316, 331, 390, 429, etc.

phenolic acid is a kind of organic acid containing phenolic ring, and specifically includes monohydroxybenzoic acid (p-hydroxybenzoate, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate), bishydroxybenzoic acid (gentisic acid, protocatechuic acid) and trihydroxybenzoic acid (gallic acid, phloroglucinol acid), etc.

in the present invention, the extraction method of the organic complex acid is the prior art, and the present invention is not limited to this, and for example, the organic complex acid can be obtained by the following method: extracting weathered lignite, screening ore, crushing, bioleaching, and obtaining filtrate which is organic composite acid.

The organic composite acid of the invention is extracted from the leonardite, so the organic composite acid contains a plurality of phenolic acids which have differences in molecular weight and structure and have molecular weight of less than or equal to 450, and the organic composite acid also contains other natural components.

The phenolic acid in the biological antidote provided by the invention can complex and chelate metal cations in wastewater, so that the phenolic acid cannot directly contact with microorganisms, the microorganisms are prevented from being poisoned, the activity of the microorganisms is ensured, in a dispersion system, the phenolic acid and certain high molecular substances form polyelectrolyte (the polyelectrolyte is long-chain polymer with ionizable groups, and the polymer can be ionized in a polar solvent to charge the high molecular chains and has flocculation property), and the biological antidote has the functions of coagulation, peptization, dispersion and the like, and in addition, the phenolic acid contains a certain amount of energy groups and has physiological activity. On the basis, the molecular weight of phenolic acid is controlled to be less than or equal to 450, only organic phenolic acid with small enough can be used as a carrier of nutrient substances to enter a cell body, and then other natural components in organic composite acid can be matched, and the natural components can generate auxiliary action to be matched with the phenolic acid, so that the biological antidote disclosed by the invention can effectively improve the oxygen consumption rate of microorganisms and the pollutant degradation capability of activated sludge, is simple in production process and low in operation cost, and can be widely applied to an industrial wastewater treatment biochemical system.

The mining place of the weathered lignite is not particularly limited, and the weathered lignite can be mined from mines in southwest of entada, as long as the extracted organic composite acid contains phenolic acid with the molecular weight of less than or equal to 450, and the weathered lignite falls into the protection scope of the invention.

The mass percentage of the organic composite acid in the biological antidote is 1% -10%, such as 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.8%, 2.0%, 2.5%, 2.8%, 3.0%, 3.2%, 3.5%, 3.8%, 4.0%, 4.5%, 4.8%, 5.0%, 5.6%, 5.9%, 6.0%, 6.5%, 7.0%, 7.5%, 8.0%, 8.5%, 9.0%, 9.5%, 9.8%, etc.

The mass percentage of the carbohydrate to the biological antidote is 3% -6%, such as 3.2%, 3.5%, 3.8%, 4.0%, 4.5%, 4.8%, 5.0%, 5.6%, 5.9% and the like.

the mass percentage of the algae extract in the biological antidote is 0.5% -1%, such as 0.6%, 0.7%, 0.8%, 0.9% and the like.

The mass percentage of the enzyme in the biological antidote is 0.2-0.5%, such as 0.22%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.48% and the like.

The surfactant accounts for 0.5-1% of the biological antidote by mass, such as 0.6%, 0.7%, 0.8%, 0.9% and the like.

The mass percentage of the trace elements in the biological antidote is 0.06% -0.12%, such as 0.07%, 0.08%, 0.09%, 0.10%, 0.11% and the like.

preferably, the mass percentage of the organic complex acid in the biological antidote is 1-5%, such as 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 2.0%, 2.1%, 2.2%, 2.5%, 2.8%, 3.0%, 3.2%, 3.5%, 3.8%, 4.0%, 4.2%, 4.6%, 4.8%, 4.9%, etc.

In the invention, the organic composite acid accounts for 1-5% of the biological antidote by mass preferably, and in the specific range, the organic composite acid can be better matched with other components, so that the pollutant degrading capability of the activated sludge is further improved.

Preferably, the mass percentage of phenolic acid in the organic composite acid is 8% -15%, such as 9%, 10%, 11%, 12%, 13%, 14%, etc., preferably 10%.

The invention preferably selects the specific mass percentage of phenolic acid in the organic composite acid, can further improve the oxygen consumption rate of microorganisms, and improves the pollutant degradation capability of activated sludge. Too much phenolic acid easily causes excessive metabolism of microorganisms, autoxidation leads to cell decomposition, and too little phenolic acid cannot achieve the effects of efficiently conveying nutrient substances and relieving toxic substances.

Preferably, the organic composite acid also comprises humic acid with molecular weight less than or equal to 2000, such as 100, 110, 120, 150, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, 1600, 1700, 1800, 1900 and the like.

Humic acid is a kind of organic substance which is produced and accumulated by remains of animals and plants, mainly remains of plants through the decomposition and transformation of microorganisms and a series of geochemical processes, and the basic structure of the humic acid is aromatic ring and alicyclic ring, and the ring is connected with functional groups such as carboxyl, hydroxyl, carbonyl, quinonyl, methoxyl and the like.

The organic composite acid preferably also comprises humic acid with the molecular weight less than or equal to 2000, and the humic acid with the specific molecular weight and phenolic acid with the specific molecular weight act together to further complex and chelate metal cations in the wastewater, so that the oxygen consumption rate of microorganisms is further increased, and the capacity of activated sludge for degrading pollutants is improved.

Since the organic composite acid of the present invention is extracted from leonardite, in a preferred embodiment, the organic composite acid contains a plurality of humic acids which are different in molecular weight and structure and have molecular weight of 2000 or less.

preferably, the organic composite acid has a humic acid content of 30% to 50% by mass, for example, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, etc., preferably 40%.

According to the invention, the specific mass percentage of humic acid in the organic composite acid is optimized, so that the oxygen consumption rate of microorganisms can be further improved, and the pollutant degradation capability of activated sludge is improved. The humic acid is too much and easy, the microorganism metabolism is vigorous, unnecessary sludge aging is caused, and the humic acid is too little, so that the effect of detoxification is not achieved.

Preferably, the mass percent of phenolic acid in the organic composite acid is 8-15%, and the mass percent of humic acid in the organic composite acid is 30-50%.

Preferably, the carbohydrate material comprises rhamnose and/or molasses, preferably rhamnose and molasses.

preferably, the percentage by mass of rhamnose in the biological antidote is 1% to 1.5%, such as 1.1%, 1.2%, 1.3%, 1.4%, etc., and the percentage by mass of molasses in the biological antidote is 2% to 4.5%, such as 2.1%, 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%, 3.0%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7%, 3.8%, 3.9%, 4.0%, 4.1%, 4.2%, 4.3%, etc.

Preferably, the algae extract comprises an extract of chlorella in the ocean and/or an extract of chlorella in the ocean.

Preferably, the algal extract has a protein content of 40% or more, such as 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, and the like.

preferably, the algae extract further contains any one or a combination of at least two of chlorophyll, vitamins, minerals and nucleic acids.

Preferably, the enzyme comprises a hydrolase and/or a lyase, preferably a hydrolase and a lyase.

preferably, the mass percentage of the hydrolase in the biological antidote is 0.15% -0.35%, such as 0.16%, 0.17%, 0.18%, 0.19%, 0.20%, 0.21%, 0.22%, 0.23%, 0.24%, 0.25%, 0.26%, 0.27%, 0.28%, 0.29%, 0.30%, 0.31%, 0.32%, 0.33%, 0.34%, etc., and the mass percentage of the lyase in the biological antidote is 0.05% -0.15%, such as 0.06%, 0.07%, 0.08%, 0.09%, 0.10%, 0.11%, 0.12%, 0.13%, 0.14%, etc.

Preferably, the surfactant comprises an anionic surfactant comprising ethylenediaminetetraacetic acid (EDTA).

Preferably, the mass percentage of the ethylene diamine tetraacetic acid in the anionic surfactant is 0.1%.

preferably, the trace elements include any one or a combination of at least two of cobalt, selenium, zinc, boron, molybdenum and manganese.

Preferably, the biological antidote comprises 0.01-0.02% by mass of cobalt, such as 0.011%, 0.012%, 0.013%, 0.014%, 0.015%, 0.016%, 0.017%, 0.018%, 0.019%, etc., the biological antidote comprises 0.01-0.02% by mass of selenium, such as 0.011%, 0.012%, 0.013%, 0.014%, 0.015%, 0.016%, 0.017%, 0.018%, 0.019%, etc., the biological antidote comprises 0.01-0.02% by mass of zinc, such as 0.011%, 0.012%, 0.013%, 0.014%, 0.015%, 0.016%, 0.017%, 0.018%, 0.019%, etc., the biological antidote comprises 0.01-0.02% by mass of boron, such as 0.011%, 0.012%, 0.013%, 0.019%, 0.014%, 0.018%, 0.019%, 0.02% by mass of molybdenum, such as 0.011%, 0.01-0.011%, 0.01% by mass of molybdenum, 0.011%, etc., the biological antidote is preferably, 0.013%, 0.014%, 0.015%, 0.016%, 0.017%, 0.018%, 0.019% and the like, wherein the mass percentage of manganese in the biological antidote is 0.01-0.02%, such as 0.011%, 0.012%, 0.013%, 0.014%, 0.015%, 0.016%, 0.017%, 0.018%, 0.019% and the like.

preferably, the biological antidote further comprises 3% -5.5% of an inorganic salt, such as 3.1%, 3.2%, 3.3%, 3.5%, 3.8%, 4.0%, 4.2%, 4.5%, 4.8%, 5.0%, 5.1%, 5.2%, 5.4%, etc.

Preferably, the inorganic salt comprises sodium sulfite and/or potassium dihydrogen phosphate, preferably sodium sulfite and potassium dihydrogen phosphate.

Preferably, the mass percentage of the sodium sulfite in the biological antidote is 2% to 3.5%, such as 2.1%, 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%, 3.0%, 3.1%, 3.2%, 3.4%, etc., and the mass percentage of the potassium dihydrogen phosphate in the biological antidote is 1% to 2%, such as 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, etc.

Preferably, the biological antidote further comprises 0.01% -0.02% of a growth factor, such as 0.011%, 0.012%, 0.013%, 0.014%, 0.015%, 0.016%, 0.017%, 0.018%, 0.019%, etc.

Preferably, the growth factor comprises any one or a combination of at least two of vitamins, bases, biotin and niacin.

Preferably, the water is deionized water.

The second object of the present invention is to provide the use of the biological antidote according to the first object for wastewater treatment.

preferably, the biological antidote is used in biochemical systems for industrial wastewater treatment.

Compared with the prior art, the invention has the following beneficial effects:

the phenolic acid in the biological antidote provided by the invention can complex and chelate metal cations in wastewater, so that the phenolic acid cannot directly contact with microorganisms, the microorganisms are prevented from being poisoned, the activity of the microorganisms is ensured, the phenolic acid serves as polyelectrolyte in a dispersion system, and the biological antidote has the functions of coagulation, peptization, dispersion and the like, and in addition, a certain amount of free radicals have physiological activity. On the basis, the molecular weight of phenolic acid is controlled to be less than or equal to 450, and other natural components in the organic composite acid are matched, and the natural components can generate auxiliary action and are matched with the phenolic acid, so that the biological antidote disclosed by the invention can effectively improve the oxygen consumption rate of microorganisms and the pollutant degradation capability of activated sludge, the production process is simple, the operation cost is low, the biological antidote can be widely applied to an industrial wastewater treatment biochemical system, and the Chemical Oxygen Demand (COD) of effluent of a secondary sedimentation tank can be reduced to be below 100 mg/L.

Drawings

FIG. 1 is a liquid chromatography mass spectrometry characterization chart of the organic complex acid provided in example 1 of the present invention.

FIG. 2 is a peak diagram of the main effective substance at 330nm in the organic composite acid provided in example 1 of the present invention.

FIG. 3 is a graph showing the change of COD of effluent from a regulating tank and COD of effluent from a secondary sedimentation tank with time, when the biological antidote provided in example 1 of the present invention is used for wastewater treatment.

FIG. 4 is a graph of the oxygen consumption rate of microorganisms in activated sludge as a function of time when the biological antidote provided in example 1 of the present invention is used for wastewater treatment.

Detailed Description

For the purpose of facilitating an understanding of the present invention, the present invention will now be described by way of examples. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.

Example 1

The embodiment provides a biological antidote, which comprises the following components in percentage by mass:

In this embodiment, the organic composite acid is extracted from leonardite mined from the mine in southwest of edawa, where the leonardite has a layered structure and is light and contains a large amount of small molecular phenolic acid and humic acid, and after the leonardite is mined, the filtrate obtained by screening, crushing and bioleaching is the organic composite acid.

Performing component analysis on the extracted organic composite acid, performing one-time C18 column separation by using a liquid chromatography mass spectrometer, performing ultraviolet spectrum absorption and mass number detection, and performing basic substance discrimination analysis by using the 330nm absorption value of the obtained phenolic acid characteristic spectrum and the corresponding molecular ion mass number value, wherein the detailed test parameters are as follows:

A chromatographic column: agilent Zorbax eclipss XDB-C184.6 x 50mm 1.8 um;

Sample introduction amount: 3 uL;

Mobile phase: 0.1% acetic acid water solution, 100% acetonitrile;

Flow rate: 0.2 mL/min;

Elution gradient: 0min B10% - >5min 15% - >20min 15% - >25min 25% - >30min 10%;

An ultraviolet array detector: DAD 221, 254,280,310,330 nm;

Measuring range of the quality detector: the mass range is 90-1500;

ESI parameters of an electrospray ion source are that the atomization pressure is 40psi, and the dry gas N ₂ 350C 9L/min;

Channel voltage: vcap 3500V, voltage 160V, skimmer 65V, Oct RF Vpp 750V;

Scanning mode: positive ms scan mode 2GHz Low (1700).

And (3) detection results:

With 330nm as a characteristic value, the retention time detected in the sample is 2.407; 3.060, respectively; 3.407 (shown in figure 1). 194.946 corresponds to the three absorption peaks; 291.082, respectively; 314.904, respectively; 216.926, respectively; 96.967, respectively; 428.852, respectively; 344.003, respectively; 176.935, respectively; 389.054 (M-H, i.e., the molecular weight is reduced by 1) in the sample, and the mass of the phenolic acid substance with the molecular weight of 450 or less is shown in FIG. 2, and the total mass of the 9 phenolic acid substances is 10.05% of the organic complex acid, and is 2.21%, 0.74%, 0.58%, 0.52%, 2.74%, 0.55%, 1.14%, 1.31%, 0.26% of the organic complex acid, respectively, according to the area integral value of each ion peak.

Besides, the organic composite acid is detected to contain 40 percent of humic acid substances with the molecular weight less than or equal to 2000 by the NY/T1971-2010 method.

in the formula, the marine green algae extract is obtained by extracting through a method simulating marine environment culture, and the rest components can be obtained commercially, wherein the surfactant containing 0.1% of ethylene diamine tetraacetic acid is a cationic surfactant (basf, CAS: 69011-36-5).

Example 2

The difference from example 1 is that the amount of the organic composite acid added is 1%.

example 3

The difference from example 1 is that the amount of the organic composite acid added is 5%.

Example 4

the difference from example 1 is that the amount of the organic composite acid added is 10%.

example 5

The difference from the embodiment 1 is that the humic acid in the organic composite acid is humic acid with the molecular weight of more than 2000.

Example 6

wherein the organic complex acid is the same as in example 1.

Example 7

Wherein the organic complex acid is the same as in example 1.

Example 8

The organic complex acid was the same as in example 1.

Comparative example 1

The difference from example 1 is that the organic complex acid is replaced by equal mass of commercially available phenolic acid (molecular weight 200-

comparative example 2

the difference from example 1 is that the organic complex acid is replaced by a mixture of equal mass of commercially available phenolic acid (molecular weight 200-.

comparative example 3

The difference from the example 1 is that the organic composite acid accounts for 0.5 percent of the mass of the biological antidote.

Comparative example 4

The difference from the example 1 is that the organic composite acid accounts for 11% of the biological antidote by mass.

Performance testing

the waste water treated by a certain paper mill in a day of 30000m ³ is produced by replacing chemical raw materials in a production workshop, the new raw materials contain more sulfate, sulfide with higher concentration is generated after passing through a collecting tank, and the sulfide in the effluent of an adjusting tank reaches 112mg/L after analysis, so that the activated sludge poisoning in a biochemical system is caused, and the specific symptoms are that the activated sludge color becomes black, filamentous fungi swell, the COD (chemical oxygen demand) of the effluent of a secondary sedimentation tank is increased, the deep treatment consumption is increased, and the operation cost is increased.

(1) The paper mill wastewater was treated and analyzed using the biological antidotes of examples and comparative examples, respectively, by the following specific treatment methods:

Diluting the biological antidote by 100 times, and continuously adding the diluted biological antidote into an aerobic tank through a metering pump for six days, wherein the adding amount of the biological antidote is 60L every day from the first day to the fifth day, the total adding amount of the biological antidote is 300L in five days, and the adding amount of the biological antidote is 30L in the sixth day;

Measuring the COD value of the effluent of the secondary sedimentation tank and the COD value of the effluent of the regulating tank after fifteen days from the first addition of the biological antidote by a national standard method GB 11914-89, and calculating the reduction amount of the COD value of the effluent of the secondary sedimentation tank relative to the COD value of the effluent of the regulating tank, wherein the results are shown in Table 1;

For the biological antidote of example 1, the effluent COD of the conditioning tank and the effluent COD of the secondary sedimentation tank at different times were recorded, as shown in fig. 3.

(2) Activated sludge analysis

Firstly, the activated sludge added with the biological antidote of the embodiment and the comparative example for 24 hours is analyzed, and the analysis method adopts an activated sludge respirator to directly test data, and the types of the instruments are as follows: SB 25-STRATTHTOX (product of Strathkelvin, UK), the oxygen consumption rate of the microorganisms was obtained as shown in Table 1;

secondly, the industrial wastewater with the sulfide content of 100mg/L is treated by using the biological antidote in the example 1, the adding amount of the biological antidote is 2ppm, the change of the oxygen consumption rate of microorganisms in the activated sludge along with the time within 2 days is recorded, and a blank control group is set: the results are shown in FIG. 4, using a solution of humic acid (molecular weight 300-.

TABLE 1

As can be seen from Table 1, after the sewage is treated by using the biological antidote of the embodiment, the COD value of the effluent of the secondary sedimentation tank is lower, and is greatly reduced compared with the COD of the effluent of the regulating tank, the COD reduction amount is 79.3-86.8%, and the microorganism activity in the activated sludge is high, and the oxygen consumption rate is 75-128 mg/L;

Comparative example 1 compared to example 1, only the organic complex acid was replaced with commercially available phenolic acid, but the biological antidote was less effective in treating wastewater, the activity of microorganisms in the activated sludge was low, the COD reduction was only 76.8%, and the oxygen consumption rate was only 69 mg/L; comparative example 2, compared to example 1, only the organic composite acid is replaced by a mixture of commercially available phenolic acid and humic acid, and the ratio of the phenolic acid to the humic acid is ensured to be the same as that of example 1, but the biological antidote has poor sewage treatment effect, the microbial activity in the activated sludge is low, the COD reduction is only 77.9%, and the oxygen consumption rate is only 70mg/L, so that the effect of the invention cannot be achieved by only phenolic acid or the mixture of phenolic acid and humic acid, therefore, other unknown natural products besides the phenolic acid and humic acid in the organic composite acid of the invention also play a crucial role in the treatment effect of the biological antidote;

Comparative examples 3 and 4 only changed the proportion of the organic composite acid in the biological antidote to 0.5% and 11% relative to example 1, but the sewage treatment effect of the biological antidote of comparative examples 3 and 4 was deteriorated, the COD reduction was reduced to 75.3% and 78.4%, respectively, and the microbial activity in the activated sludge was low, and the oxygen consumption rate was reduced to 62mg/L and 73mg/L, respectively, and therefore, the addition amount of the organic composite acid also had a large influence on the use effect of the biological antidote;

in conclusion, the biological antidote is prepared by using the organic composite acid extracted from the weathered lignite, the molecular weight of phenolic acid in the biological antidote is ensured to be less than or equal to 450, and the addition amount of the organic composite acid is controlled, so that the biological antidote can effectively improve the oxygen consumption rate of microorganisms and the pollutant degradation capability of activated sludge.

fig. 3 is a graph showing the change of COD of effluent from a regulating reservoir and COD of effluent from a secondary sedimentation tank with time when the biological antidote provided in example 1 is used for wastewater treatment, wherein the COD of effluent from the secondary sedimentation tank is reduced to less than 100mg/L and is kept stable all the time after the COD of effluent from the primary sedimentation tank is reduced to less than 100mg/L on the 45 th day shown by the abscissa, namely the first day when the biological antidote is added, and is 60 th day shown by the abscissa, namely the 15 th day after the biological antidote is added, which proves that the biological antidote provided by the present invention can improve the pollutant degrading capability of activated sludge.

As shown in fig. 4, the oxygen consumption rate of the microorganisms in the activated sludge of the experimental group (added with the biological antidote) is gradually increased from time to time, and is sharply increased at about 1.25 days, and the oxygen consumption rate is as high as about 140mg/L at 2 days, while the oxygen consumption rate of the microorganisms in the activated sludge of the blank control group (added with the humic acid solution) is very weak as time to be always kept at a level of 20-30mg/L, thereby proving that the biological antidote provided by the invention can obviously improve the oxygen consumption rate of the microorganisms in the activated sludge, and further improve the capacity of degrading pollutants.

It is understood from comparative examples 1 to 4 that the effect is best when the mass percentage of the organic composite acid in the biological antidote is 1 to 5% (examples 1 to 3), and that the sewage treatment effect of the biological antidote is deteriorated and the activity of microorganisms in the activated sludge is lowered when the mass percentage of the organic composite acid exceeds 5% (example 4).

The applicant states that the present invention is illustrated by the above examples to show the detailed process equipment and process flow of the present invention, but the present invention is not limited to the above detailed process equipment and process flow, i.e. it does not mean that the present invention must rely on the above detailed process equipment and process flow to be implemented. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

Claims

1. The biological antidote is characterized by comprising the following components in percentage by mass:

The organic composite acid is extracted from weathered lignite;

The organic composite acid contains phenolic acid with molecular weight less than or equal to 450.

2. the biological antidote according to claim 1, wherein the mass percentage of the organic complex acid in the biological antidote is 1-5%;

Preferably, the mass percent of the phenolic acid in the organic composite acid is 8% -15%, preferably 10%;

Preferably, the organic composite acid also comprises humic acid with molecular weight less than or equal to 2000;

Preferably, the mass percent of humic acid in the organic composite acid is 30-50%, preferably 40%;

3. the biological antidote according to claim 1 or 2, characterized in that the carbohydrate substances comprise rhamnose and/or molasses, preferably rhamnose and molasses;

preferably, the mass percent of rhamnose in the biological antidote is 1-1.5%, and the mass percent of molasses in the biological antidote is 2-4.5%.

4. the biological antidote according to any one of claims 1-3, wherein the algae extract comprises an extract of Chlorella maritima and/or an extract of Chlorella maritima;

preferably, the protein content in the algae extract is more than or equal to 40 percent;

5. The biological antidote according to any one of claims 1 to 4, characterized in that the enzyme comprises a hydrolase and/or a lyase, preferably a hydrolase and a lyase;

Preferably, the mass percent of the hydrolase in the biological antidote is 0.15-0.35%, and the mass percent of the lyase in the biological antidote is 0.05-0.15%.

6. the biological antidote according to any one of claims 1 to 5 wherein the surfactant comprises an anionic surfactant comprising ethylenediaminetetraacetic acid;

7. the biological antidote according to any one of claims 1-6, characterized in that the trace elements comprise any one or a combination of at least two of cobalt, selenium, zinc, boron, molybdenum and manganese;

Preferably, the mass percent of cobalt in the biological antidote is 0.01-0.02%, the mass percent of selenium in the biological antidote is 0.01-0.02%, the mass percent of zinc in the biological antidote is 0.01-0.02%, the mass percent of boron in the biological antidote is 0.01-0.02%, the mass percent of molybdenum in the biological antidote is 0.01-0.02%, and the mass percent of manganese in the biological antidote is 0.01-0.02%.

8. the biological antidote according to any one of claims 1-7, further comprising 3% -5.5% of an inorganic salt;

Preferably, the inorganic salt comprises sodium sulfite and/or potassium dihydrogen phosphate, preferably sodium sulfite and potassium dihydrogen phosphate;

preferably, the mass percent of the sodium sulfite in the biological antidote is 2-3.5%, and the mass percent of the potassium dihydrogen phosphate in the biological antidote is 1-2%.

9. the biological antidote according to any one of claims 1-8, further comprising 0.01% -0.02% growth factor;

Preferably, the growth factor comprises any one or a combination of at least two of vitamins, bases, biotin and niacin;

Preferably, the water is deionized water.

10. use of a biological antidote according to any one of claims 1-9 for wastewater treatment;