CN110547209A - Liquid feeding auxiliary device and method for mice - Google Patents

Liquid feeding auxiliary device and method for mice Download PDF

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Publication number
CN110547209A
CN110547209A CN201910891395.1A CN201910891395A CN110547209A CN 110547209 A CN110547209 A CN 110547209A CN 201910891395 A CN201910891395 A CN 201910891395A CN 110547209 A CN110547209 A CN 110547209A
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China
Prior art keywords
mouse
handle
handles
diameter
air bag
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CN201910891395.1A
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CN110547209B (en
Inventor
董贤文
王高富
张坤
付琳
张丽
李�杰
周鹏
刘作华
王贵学
黄迎春
任航行
孙晓燕
王琳
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Chongqing Academy of Animal Sciences
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Chongqing Academy of Animal Sciences
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Priority to CN201910891395.1A priority Critical patent/CN110547209B/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K5/00Feeding devices for stock or game ; Feeding wagons; Feeding stacks
    • A01K5/01Feed troughs; Feed pails
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D7/00Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Birds (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Position Input By Displaying (AREA)
  • Eye Examination Apparatus (AREA)

Abstract

The invention provides a liquid feeding auxiliary device for a mouse and a feeding method, wherein the liquid feeding auxiliary device comprises two handles, the rear end parts of the two handles are fixedly connected with the same claw torsion spring, notches are respectively arranged on the inner side surfaces of the two handles, the notches extend backwards from the front end shaft of the handles, when the two handles move to proper positions in opposite directions, the notches on the two handles form an accommodating cavity for accommodating the neck part to the tail part of the mouse together, the diameter of the large-diameter part of the accommodating cavity is not smaller than the waistline of the mouse, and the diameter of the small-diameter part of the accommodating cavity is not larger than the diameter of the head part of the mouse and is not smaller than the diameter of. The feeding method comprises the steps of fixing the mice by adopting the device and then feeding the mice. The invention completely eliminates force error factors caused by pinching the mouse by hands, solves the technical problems that the mouse is greatly stressed when the mouse is fixed in the feeding process and the stress difference of the mouse is large when the mouse is fed for different times, and is beneficial to improving the accuracy of experimental results.

Description

Liquid feeding auxiliary device and method for mice
Technical Field
The invention relates to a mouse experimental tool, in particular to a liquid feeding auxiliary device and a liquid feeding method for a mouse.
Background
Mice were used in experiments by humans as early as the 17 th century and are now one of the most widely used and studied experimental mammals. In the research of taking a mouse as an experimental object, the liquid feed or the medicine is mainly used for capturing and fixing the mouse by manpower, and then the related liquid feed or the medicine is fed, for example, an operator holds the mouse by the left hand and stretches a material pipe into the stomach of the mouse by the right hand to feed. However, there are a number of problems associated with feeding after manually pinching the immobilized mice by hand, including: the mice are not firmly fixed and often bite operators; the feeding liquid is easy to overflow, so that the experimental result is inaccurate; different operators have different hand pinching forces, so that the mice are stressed differently, and the experimental result is inaccurate.
Document CN205922413U discloses a simple and practical liquid feed feeding device, which comprises a first nut, a gasket, a cage, a screw handle, a second nut, a drawing liquid storage tube, a universal adjusting foot and a round clamp, wherein the tube body of the drawing liquid storage tube is fixed on the round clamp, the upper part of the outer metal layer of the round clamp is connected with the universal adjusting foot, the universal adjusting foot is fixed at the lower end of the cage above the universal adjusting foot through the first nut, the gasket and the screw handle, the lower end of the screw handle is fixedly connected with the second nut, and the lower end of the universal adjusting foot is provided with the round clamp. However, this device cannot solve the technical problem of the large stress to which the mouse is subjected when the mouse is immobilized.
Disclosure of Invention
The invention aims to provide a liquid feeding auxiliary device for mice, which aims to solve the technical problems that the mice are greatly stressed when being fixed in the feeding process, and the mice are greatly stressed differently when being fed for different times.
In order to achieve the above object, the present invention adopts the following technical solutions.
Mouse liquid irritates and feeds auxiliary device, including two handles (handle one and handle two), the same claw torsional spring of back tip fixed connection of two handles, the medial surface at two handles is provided with the breach respectively, the breach extends behind the handle front end axial, after two handles move to suitable position in opposite directions, breach on two handles constitutes the chamber that holds mouse neck to afterbody jointly, and the major diameter portion position diameter that holds the chamber is not less than the mouse waistline, the minor diameter position diameter that holds the chamber is not more than mouse head diameter, not less than mouse neck diameter.
In order to further reduce the stress on the mouse in the fixing process, an inflatable air bag is arranged at the middle upper part of the notch on the inner side surface of the handle, and the air bag is inflated and expanded to form an air bag which is directly contacted with the body of the mouse.
In order to further reduce the stress on the mice in the fixing process, the air bag is made of natural rubber.
In order to facilitate smooth feeding, the wall thickness of the upper part of the air bag is smaller than that of the lower part of the air bag, namely, the wall thickness of the air bag is gradually increased from the upper part of the air bag to the lower part of the air bag. The structure is convenient for feeding, because the wall thickness of the upper part of the air bag is the minimum, when the air bag is inflated, the upper part of the air bag expands first, and the expanding part slightly presses the back of the mouse gradually, so that the belly of the mouse is gradually attached to the bottom of the accommodating cavity, and the body of the mouse can be adjusted to be in a horizontal state as far as possible.
For convenient operation, the air bag is connected with a hand-pinch inflator pump which is fixed on the outer side wall of the handle.
In order to facilitate accurate control of stress on the mouse during fixation, a plurality of independent thermocouple temperature measuring wires are laid on the inner wall of the notch at intervals, and the thermocouple temperature measuring wires extend downwards along the middle of the notch to the bottom of the notch and then are externally connected with a temperature display end.
Furthermore, a hook-shaped rod is arranged on the inner side surface of the first handle, a hole is formed in the inner side surface of the second handle, which is positioned on the right opposite side of the hook-shaped rod, a tooth part is arranged in the hole, the lower end of the tooth part is connected with a spring, the spring is fixed on the bottom wall of the hole, the upper end of the tooth part is connected with a button, and the button movably extends out of the upper end; when the first handle and the second handle move towards each other, the hook-shaped rod can extend into the hole and be hooked on the teeth of the tooth part so as to realize the mutual locking of the first handle and the second handle; when the button is pressed, the teeth are disengaged from the hook bar to effect separation of the first and second handles from each other. Preferably, the gap between adjacent teeth on the tooth part is 3-5 mm.
the invention also aims to provide a liquid feeding method for mice, which comprises the following steps:
step 1: grabbing a mouse which meets the experimental requirements, and holding the handle of the liquid feeding auxiliary device of the mouse in an unlocked state by a hand;
Step 2: placing the neck to the tail of the mouse into a gap between the first handle and the second handle, exposing the head of the mouse, applying force by the right hand to hold and press the first handle and the second handle to enable the first handle and the second handle to move to proper positions oppositely, stopping applying force by the right hand, and horizontally placing the handles on an operation table top;
And step 3: holding the hand-pinch inflator to repeatedly and continuously perform pinching-pressing-releasing operation, observing the temperature display ends, and stopping pressing the hand-pinch inflator corresponding to the temperature display end when a certain temperature display end displays 35-38 ℃ until all the temperature display ends display 35-38 ℃;
And 4, step 4: inserting the feeding pipe meeting the requirements into the stomach of the mouse to feed liquid; and after the feeding is finished, pressing the button to separate the first handle and the second handle, and putting the mouse back to the specified position.
preferably, in step 3, the time for each operation of "kneading-releasing" is controlled to be 2 to 3 seconds.
The mouse is limited by the containing cavity with a specific structure, the air bag after the air bag is inflated and expanded is contacted with and presses down the body of the mouse, and the body temperature of the mouse is sensed by combining the thermocouple temperature measuring line to judge whether the mouse adheres to the wall or not, so that force error factors caused by pinching the mouse by hands are completely eliminated, the technical problems that the mouse is stressed greatly when being fixed in the feeding process and the stress difference of the mouse is large when being fed for different times are solved, the accuracy of an experimental result is improved, the posture of the mouse can be quickly adjusted, the mouse is in a horizontal state as far as possible, the smooth feeding of the mouse is realized, the mouse can be effectively fixed, and operators are prevented from being bitten by the mouse in the feeding process.
Drawings
FIG. 1 is a schematic diagram of the liquid feeding aid for mice in the examples;
FIG. 2 is a schematic view of a first handle of the device of FIG. 1;
FIG. 3 is a schematic view of the hook-shaped lever of the device of FIG. 1 hooked over the teeth;
FIG. 4 is a schematic view of the hook lever of the device of FIG. 1 disengaged from the teeth;
FIG. 5 is a schematic view of the air bag of the device of FIG. 1 in connection with a hand-pinch inflator;
FIG. 6 is a schematic sectional view of the mouse liquid feeding aid in the example for fixing the mouse.
Detailed Description
the present invention will be further described with reference to the accompanying drawings and specific embodiments, but the following embodiments are only used for understanding the principle of the present invention and the core idea thereof, and do not limit the scope of the present invention. It should be noted that modifications to the invention as described herein, which do not depart from the principles of the invention, are intended to be within the scope of the claims which follow.
Example 1
as shown in fig. 1, the auxiliary device for liquid feeding of mice comprises a first handle 3 and a second handle 4, wherein the rear end parts of the first handle and the second handle are fixedly connected with the same pawl torsion spring 1, namely the rear end parts of the first handle 3 and the second handle 4 are fixedly connected with two pawls of the same pawl torsion spring 1; a limiting plate 2 is arranged between the rear end part of the handle and the claw torsion spring 1, two waist holes are arranged on the limiting plate 2, and two claws of the claw torsion spring 1 are connected with the handle after respectively penetrating through the corresponding waist holes. Be provided with breach 11 respectively at the medial surface of two handles, breach 11 is prolonged backward from the handle front end axial, and after two handles move to suitable position in opposite directions, breach 11 on two handles constitutes jointly and holds the chamber that holds that mouse neck to afterbody, and holds the major diameter position diameter in chamber and be not less than 20 waistlines of mouse, and the minor diameter position diameter that holds the chamber is not more than 20 head diameters of mouse, is not less than 20 neck diameters of mouse.
As shown in fig. 1 and 2, an inflatable air bag 7 is arranged at the middle upper part of a notch 11 on the inner side surface of the handle, and the air bag 7 forms an air bag which is directly contacted with the body of a mouse 20 after being inflated and expanded. Specifically, a certain margin is left on the top of the air bag 7 and the bottom of the reserved air bag 7 respectively, the margin is equivalent to the wide side of the air bag 7, and the margin part cannot be communicated with the inner cavity of the air bag 7, so that the air bag 7 can be installed. Adopt clamp plate 12 and countersunk screw 13 tightly to press fitting the air pocket 7 top at the 3 inside walls of handle of breach 11 top, clamp plate 12 is a flat board of taking the counter bore, adopts clamp plate 14 and countersunk screw to tightly press fitting the air pocket 7 bottom at breach 11 middle part, and clamp plate 14 is for matcing the arc in 11 internal face of breach, is provided with the counter bore on the arc and is used for installing the countersunk screw.
In this embodiment, the air bag 7 is made of natural rubber, and the wall thickness of the upper part of the air bag 7 is smaller than that of the lower part of the air bag 7. Specifically, the wall thickness of the upper part of the air bag 7 can be 0.04-0.07mm, the wall thickness of the lower part and the bottom part of the air bag 7 can be 0.15-0.3mm, and the wall thickness of the middle part of the air bag 7 is between 0.09-0.12 mm. The structure is convenient for feeding, because the wall thickness of the upper part of the air bag 7 is the minimum, when the air bag 7 is inflated, the upper part of the air bag 7 can be firstly expanded, the expanded part gradually and slightly presses the back of the mouse 20, so that the belly of the mouse 20 gradually clings to the bottom of the accommodating cavity, and the body of the mouse 20 can be adjusted to be in a horizontal state as far as possible. The state of the airbag 7 inflated and pressed down the mouse 20 is shown in fig. 6.
In this embodiment, as shown in fig. 5 and 6, the air bag 7 is connected to the hand-pinch inflator 5, and the hand-pinch inflator 5 is fixed on the outer sidewall of the handle. When the air bag 7 needs to be inflated, the hand-pinch inflator 5 only needs to be held to repeatedly and continuously perform the pinching-releasing operation.
in this embodiment, as shown in fig. 2, a plurality of independent thermocouple temperature measuring lines 10 are further laid on the inner wall of the notch 11 at intervals, and the thermocouple temperature measuring lines 10 extend downwards along the middle of the notch 11 to the bottom of the notch 11 and then are externally connected to the temperature display end 6. Specifically, the thermocouple temperature measuring line 10 may be a commercially available ultra-fine K-type thermocouple temperature measuring line with a line diameter of 0.1mm, or a commercially available ultra-fine T-type thermocouple temperature measuring line with a line diameter of 0.08mm, and the temperature sensing end of the thermocouple temperature measuring line 10 is adhered to the corresponding position of the inner wall of the notch 11 by an adhesive tape during installation.
In the embodiment, as shown in fig. 1, 3 and 4, a hook rod 9 is arranged on the inner side surface of a first handle 3, a hole 18 is arranged on the inner side surface of a second handle 4 which is positioned on the opposite side of the hook rod 9, a tooth part 16 is arranged in the hole 18, the gap between adjacent teeth 15 on the tooth part 16 is 3-5mm, the lower end of the tooth part 16 is connected with a spring 17, the spring 17 is fixed on the bottom wall of the hole 18, the upper end of the tooth part 16 is connected with a button 8, and the button 8 movably extends out of the upper end surface of; when the first handle 3 and the second handle 4 move towards each other, the hook rod 9 can extend into the hole 18 and be hooked on the teeth 15 of the tooth part 16 to realize the mutual locking of the first handle 3 and the second handle 4; when the button 8 is pressed, the teeth 16 are disengaged from the hook bar 9 to effect the separation of the first and second handles 3 and 4 from each other.
In the embodiment, the size of the accommodating cavity can be determined according to the body size specification of a conventional mouse, the waist circumference of the mouse with the weight of 17.9-32.2g is 43-49 mm, and the neck diameter is 9.9-12.1 mm. Therefore, when the first handle 3 and the second handle 4 are locked with each other, the diameter of the large-diameter part of the accommodating cavity formed by the notches 11 on the two handles is 53-57mm, and the diameter of the small-diameter part of the accommodating cavity is 13-15 mm.
Example 2
A method of liquid feeding mice using the apparatus of example 1, comprising the steps of:
step 1: grabbing a mouse 20 meeting the experimental requirements, and holding a handle of the liquid feeding auxiliary device of the mouse in an unlocked state;
Step 2: placing the neck to the tail of the mouse 20 into the gap 11 between the first handle 3 and the second handle 4, exposing the head of the mouse 20, applying force by the right hand to hold and press the first handle 3 and the second handle 4 to enable the first handle 3 and the second handle 4 to move to proper positions oppositely, stopping applying force by the right hand, and horizontally placing the handles on the operating table top;
and step 3: holding the hand-pinch inflator 5 to repeatedly and continuously carry out the pinching-releasing operation, controlling the time of carrying out the pinching-releasing operation every time to be 2-3 seconds, simultaneously observing the temperature display ends 6, and stopping pressing the hand-pinch inflator 5 corresponding to the temperature display end 6 when a certain temperature display end 6 displays 35-38 ℃ until all the temperature display ends 6 display 35-38 ℃; the thermocouple temperature measuring line is very sensitive, when the skin of a mouse contacts the thermocouple temperature measuring line, the number of the temperature display end can rapidly jump until the skin temperature of the mouse is displayed, when all the temperature display ends display 35-38 ℃, the lower surface of the mouse is shown to be attached to the bottom wall of the accommodating cavity, at the moment, the body of the mouse is in a non-curling fixed state, the feeding auxiliary device is in a horizontal state, and the feeding auxiliary device is in an optimal state suitable for feeding liquid; in the step, the pinching-releasing operation is to slightly press the body of the mouse by adopting the air bag inflated and expanded air bag, the mouse body is slightly stressed in a mode of pressing the body of the mouse downwards, the influence on the mouse tends to be constant when the mouse is used for different times, the mouse is not influenced by the hand temperature of an operator, and the force error factor caused by manually pinching the mouse by hand is completely eliminated;
and 4, step 4: inserting the feeding pipe meeting the requirements into the stomach of the mouse 20 to feed liquid; after feeding, the button 8 is pressed down to separate the first handle 3 and the second handle 4 from each other, and the mouse 20 is returned to the designated position.
When the mouse 20 is placed in the notch 11, the abdomen of the mouse should be directed to the bottom wall of the notch 11 to prevent the mouse from scratching the air bag 7. When grasping the mouse, it is preferable to grasp the top of the mouse and locate the mouse between the ears.
Carry on spacingly through the chamber that holds that adopts specific structure to the mouse, the gasbag after adopting the air pocket to inflate the inflation contacts and pushes down the mouse health, whether the perception is carried out in order to judge the mouse adherence to the wall to the body temperature to the thermocouple temperature line, the dynamics error factor that the mouse caused has been held between the fingers to the hand for the people has been got rid of completely, it is big not only to have solved to cause the mouse to receive stress when irritating the fixed mouse of feeding in-process, the big technical problem of the stress difference that the mouse receives when irritating the feeding at different times, be favorable to improving the accuracy of experimental result, and can adjust the body appearance of mouse fast, make the mouse be in the horizontality as far as possible, realize irritating the feeding smoothly to the mouse, can also effectively fix the mouse, avoid irritating the mouse of.

Claims (10)

1. Mouse liquid is irritated and is fed auxiliary device, its characterized in that: the device includes handle one (3) and handle two (4), same claw torsional spring (1) of back tip fixed connection of two handles, medial surface at two handles is provided with breach (11) respectively, breach (11) extend behind the handle front end axial, after two handles move to suitable position in opposite directions, breach (11) on two handles constitute jointly and hold mouse neck to the chamber that holds of afterbody, and the major diameter portion position diameter that holds the chamber is not less than mouse (20) waistline, the minor diameter position diameter that holds the chamber is not more than mouse (20) head diameter, not less than mouse (20) neck diameter.
2. the apparatus of claim 1, wherein: an inflatable air bag (7) is arranged at the middle upper part of a notch (11) on the inner side surface of the handle, and the air bag (7) forms an air bag which is directly contacted with the body of a mouse (20) after being inflated and expanded.
3. The apparatus of claim 2, wherein: the air bag (7) is made of natural rubber.
4. The apparatus of claim 2 or 3, wherein: the wall thickness of the upper part of the air bag (7) is smaller than that of the lower part of the air bag (7).
5. the apparatus of claim 4, wherein: the air bag (7) is connected with the hand-pinch inflator pump (5), and the hand-pinch inflator pump (5) is fixed on the outer side wall of the handle.
6. The apparatus of claim 5, wherein: a plurality of independent thermocouple temperature measuring lines (10) are laid on the inner wall of the notch (11) at intervals, and the thermocouple temperature measuring lines (10) extend downwards to the bottom of the notch (11) along the middle of the notch (11) and then are externally connected with a temperature display end (6).
7. the apparatus of claim 6, wherein: a hook rod (9) is arranged on the inner side surface of the first handle (3), a hole (18) is formed in the inner side surface of the second handle (4) which is located on the opposite side of the hook rod (9), a tooth part (16) is arranged in the hole (18), the lower end of the tooth part (16) is connected with a spring (17), the spring (17) is fixed on the bottom wall of the hole (18), the upper end of the tooth part (16) is connected with a button (8), and the button (8) movably extends out of the upper end surface of the second handle (4; when the first handle (3) and the second handle (4) move towards each other, the hook rod (9) can extend into the hole (18) and be hooked on the teeth (15) of the teeth part (16) to realize the mutual locking of the first handle (3) and the second handle (4); when the button (8) is pressed down, the tooth part (16) is disengaged from the hook rod (9) to realize the mutual separation of the first handle (3) and the second handle (4).
8. The apparatus of claim 7, wherein: the gap between adjacent teeth (15) on the tooth part (16) is 3-5 mm.
9. A method of liquid feeding mice, characterized in that the device of claim 6 or 7 is used, comprising the steps of:
Step 1: grabbing a mouse (20) meeting the experimental requirements, and holding a handle of the device in a non-locking state;
step 2: placing the neck to the tail of the mouse (20) into a gap (11) between the first handle (3) and the second handle (4), exposing the head of the mouse (20), applying force to hold and pressing the first handle (3) and the second handle (4) by the right hand to enable the first handle (3) and the second handle (4) to move to proper positions oppositely, stopping applying force by the right hand, and flatly placing the first handle on the operating table top;
And step 3: holding the hand-pinch inflator (5) to repeatedly and continuously carry out pinching-releasing operation, observing the temperature display ends (6), and stopping pressing the hand-pinch inflator (5) corresponding to the temperature display end (6) when a certain temperature display end (6) displays 35-38 ℃ until all the temperature display ends (6) display 35-38 ℃;
And 4, step 4: inserting the feeding pipe meeting the requirements into the stomach of a mouse (20) for liquid feeding; after feeding, the button (8) is pressed down to separate the first handle (3) and the second handle (4) from each other, and the mouse (20) is placed back to the designated position.
10. The method of claim 9, wherein: in the step 3, the time for each pinching-releasing operation is controlled to be 2-3 seconds.
CN201910891395.1A 2019-09-20 2019-09-20 Liquid feeding auxiliary device and method for mice Active CN110547209B (en)

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Application Number Priority Date Filing Date Title
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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3184937U (en) * 2013-05-13 2013-07-25 ハムリー株式会社 Small animal restraint
CN205054490U (en) * 2015-05-14 2016-03-02 山东大学第二医院 Rat trapper
CN105496596A (en) * 2016-02-23 2016-04-20 孟超 Experimental mouse fixing device
CN205181526U (en) * 2015-12-01 2016-04-27 上海市第一人民医院 Fixing device is handled to medical experiment mouse
CN206391044U (en) * 2016-08-02 2017-08-11 齐齐哈尔医学院 Experiment mice inflatable fixing device
CN208481523U (en) * 2017-10-31 2019-02-12 刘倩 Rat oral gavage assistor

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3184937U (en) * 2013-05-13 2013-07-25 ハムリー株式会社 Small animal restraint
CN205054490U (en) * 2015-05-14 2016-03-02 山东大学第二医院 Rat trapper
CN205181526U (en) * 2015-12-01 2016-04-27 上海市第一人民医院 Fixing device is handled to medical experiment mouse
CN105496596A (en) * 2016-02-23 2016-04-20 孟超 Experimental mouse fixing device
CN206391044U (en) * 2016-08-02 2017-08-11 齐齐哈尔医学院 Experiment mice inflatable fixing device
CN208481523U (en) * 2017-10-31 2019-02-12 刘倩 Rat oral gavage assistor

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