CN110518273A - A kind of single-chamber microbial fuel cell and preparation method thereof for the research of electro-chemical activity bacterium group - Google Patents
A kind of single-chamber microbial fuel cell and preparation method thereof for the research of electro-chemical activity bacterium group Download PDFInfo
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- CN110518273A CN110518273A CN201910814486.5A CN201910814486A CN110518273A CN 110518273 A CN110518273 A CN 110518273A CN 201910814486 A CN201910814486 A CN 201910814486A CN 110518273 A CN110518273 A CN 110518273A
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M4/00—Electrodes
- H01M4/86—Inert electrodes with catalytic activity, e.g. for fuel cells
- H01M4/88—Processes of manufacture
- H01M4/8878—Treatment steps after deposition of the catalytic active composition or after shaping of the electrode being free-standing body
- H01M4/8896—Pressing, rolling, calendering
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M4/00—Electrodes
- H01M4/86—Inert electrodes with catalytic activity, e.g. for fuel cells
- H01M4/90—Selection of catalytic material
- H01M4/9075—Catalytic material supported on carriers, e.g. powder carriers
- H01M4/9083—Catalytic material supported on carriers, e.g. powder carriers on carbon or graphite
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M4/00—Electrodes
- H01M4/86—Inert electrodes with catalytic activity, e.g. for fuel cells
- H01M4/96—Carbon-based electrodes
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M8/00—Fuel cells; Manufacture thereof
- H01M8/16—Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P70/00—Climate change mitigation technologies in the production process for final industrial or consumer products
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Abstract
The invention discloses a kind of single-chamber microbial fuel cells and preparation method thereof for the research of electro-chemical activity bacterium group, belong to the technical field of microorganism electrochemical.Object of the present invention is to provide a kind of single chamber air cathode microbial fuel cell production method that research is simply and effectively learned for group aiming at the problem that most microbiological fuel cells are not suitable for group learning research.The basic structure of the single-chamber microbial fuel cell for the research of electro-chemical activity bacterium group is made of carbon paper anode, air cathode and glass body.The present invention makes air cathode simplified operation process using the technique of roll-in and reduces Production Time, improves the producing efficiency of air cathode, can guarantee the pure culture environment of reactor in cathode outer side setting degerming filter membrane.It can guarantee the quantitative extraction of nucleic acid and protein sample, as anode using carbon paper convenient for subsequent high-flux sequence and group credit analysis.
Description
Technical field
The invention belongs to the technical fields of microorganism electrochemical；It is ground more particularly to one kind for electro-chemical activity bacterium group
The preparation method for the single-chamber microbial fuel cell studied carefully.
Background technique
In sewage treatment field, microbiological fuel cell (Microbial fuel cell, MFC) technology is as one
Emerging wastewater processing technology is rapidly developed, and becomes domestic and international research hotspot.Nearly ten years, with reactor configuration
With the optimization of electrode material, MFC system power, which exports, to be obviously improved.However MFC electrification after some research discovery amplifications
It learns active biological film and forms difficulty, be easy to cause power overshoot, amplify post-reactor performance well below laboratory level.Due to
Electrode microbial biofilm is formed and lacks enough understandings, therefore is changed only by the macroscopic view of electrode material and size, very
Hardly possible realizes the synchronous amplification of MFC efficiency of fuel cell generation.The electrochemistry for being mainly enriched in anode that electro catalytic activity is played in MFC is living
Property microorganism.Electron transfer rate between microorganism and anode is the key factor for influencing microorganism electrochemical system performance,
As the major reason for restricting microorganism electro-catalysis conversion efficiency.Therefore, in order to further increase system effectiveness, in biomembrane shape
Regulation is optimized at initial stage, needs to carry out extracellular electron transmission mechanism, the optimization anode table of electro-chemical activity bacterium and group
Face biomembrane structure of community etc. research.
Single chamber air cathode microbial fuel cell has good electricity production effect and simple configuration, becomes research electrification
Learn the effective tool of bacterium function.The genomics based on high throughput nucleic acid sequencing technology of rising in recent years, transcription group and
Protein science provides idea and method for characteristic and the metabolic function research of functional microorganism in varying environment.However at present
It is special not to be suitable for group research due to configuration and electrode material for most of single chamber air cathode microbial fuel cells
It is the research of pure thallus system.Therefore, the life of the Protocols in Molecular Biology research electro-chemical activity bacterium using nucleic acid sequencing is realized
Manage ecosystem characterization and metabolic pathway, it is necessary to further transformation single chamber air cathode microbial fuel cell.
Summary of the invention
Object of the present invention is to provide one kind aiming at the problem that current most microbiological fuel cells are not suitable for group learning research
The single chamber air cathode microbial fuel cell production method of research is simply and effectively learned for group.
To solve the above problems, provided by the present invention for the single-chamber microbial fuel of electro-chemical activity bacterium group research
A kind of single-chamber microbial fuel cell for the research of electro-chemical activity bacterium group of battery, including shell of reactor (1), carbon paper
Anode (2), air cathode (3), degerming filter membrane (4), conducting wire (5), resistance (6), titanium silk (7), wherein shell of reactor is on having
Lid, side wall have the glass body of circular open, and the air cathode is by powdered capacitor activated carbon, conductive black, stainless steel
Made of net and ptfe emulsion, the carbon paper anode is set to inside shell of reactor, is connect with titanium silk, and titanium silk passes through
The upper cover of shell of reactor stretches out shell of reactor, connects resistance by conducting wire, and resistance connects another section of titanium silk by conducting wire, should
The titanium silk other end is connected with air cathode；The air cathode is arranged at the circular open of shell of reactor side wall, passes through list
Flange and stainless steel folder are fixed on shell of reactor, which is completely enclosed, is provided with degerming filter membrane on the outside of air cathode.
The anode carbon paper size is (2~2.5cm) × (6~10cm).
The glass body dischargeable capacity is 200~250ml, and glass bottle opening is sealed into upper cover, bottle body using rubber rubber plug
Side circular open area is 6~8cm2。
The degerming filter sizes are 0.22 μm, and area is 8~10cm2。
In addition, the present invention also provides the above-mentioned single-chamber microbial fuel cells for the research of electro-chemical activity bacterium group
Preparation method carries out in the steps below:
Step 1: being stirred by ultrasonic after powdered capacitor activated carbon powder is mixed with dehydrated alcohol, it is slowly added to polytetrafluoroethyl-ne
Alkene lotion is evaporated to mixture to dehydrated alcohol and forms micelle, then with the continuous roll-in of roll squeezer until compacting flakiness, is made
The Catalytic Layer of air cathode；
Step 2: being stirred by ultrasonic after conductive black is mixed with dehydrated alcohol, it is slowly added to ptfe emulsion, to nothing
Water-ethanol is evaporated to mixture and forms micelle, with the continuous roll-in of roll squeezer until suppressing flakiness, then together with stainless (steel) wire
Being put into roll squeezer compacting makes it be embedded in stainless (steel) wire, and mixing thin slice is put into Muffle furnace and is heated；
Step 3: the thin slice after heating that Catalytic Layer made from step 1 is prepared with step 2 is superimposed placement, put together
Enter roll squeezer, suppresses three to four times and obtain air cathode；
Step 4: being perforated after carbon paper is cut with titanium silk, and smear conducting resinl and fix, carbon paper anode is made；
Step 5: air cathode, degerming filter membrane, carbon paper anode and glass body are assembled integrally structure, single chamber is made
Microbiological fuel cell.
In step 1 the mass ratio of capacitor activated carbon powder and dehydrated alcohol be 1:(4~6), ultrasonic agitation the time be 20~
40min, Catalytic Layer is with a thickness of 0.4~0.6mm.
Ptfe emulsion solid content is 40%~80%wt, capacitor activated carbon powder and polytetrafluoroethylene (PTFE) cream in step 1
Liquid mass ratio is (4~6): 1.
Conductive black and dehydrated alcohol mass ratio are 1:(2~3 in step 2), the ultrasonic agitation time is 15~30min, thin
Piece is with a thickness of 0.4~0.6mm.
Ptfe emulsion solid content is 40%~80%wt, conductive black and ptfe emulsion matter in step 2
Amount is than being (1~1.2): 3；The mesh number of stainless (steel) wire is 40~100 mesh.
Air cathode, degerming filter membrane, carbon paper anode and glass body are assembled integrally structure described in step 5, according to
Lower connection relationship assembling: shell of reactor is the glass body for having upper cover, side wall to have circular open, and the air cathode is by powder
Made of last shape capacitor activated carbon, conductive black, stainless (steel) wire and ptfe emulsion, the carbon paper anode is set to reaction
Device interior of shell is connect with titanium silk, and the upper cover that titanium silk passes through shell of reactor stretches out shell of reactor, connects electricity by conducting wire
Resistance, resistance connect another section of titanium silk by conducting wire, which is connected with air cathode；The air cathode is arranged anti-
At the circular open for answering device side wall of outer shell, it is fixed on shell of reactor by single flange and stainless steel folder, the opening is complete
Closing, air cathode outside are provided with degerming filter membrane.
Beneficial effect
The present invention is the single-chamber microbial fuel cell studied for electro-chemical activity bacterium group, and basic structure is by carbon
Paper anode, air cathode and glass body are constituted.Air cathode is by powdered capacitor activated carbon, conductive black, stainless (steel) wire
With made of ptfe emulsion, degerming filter membrane is arranged in outside.Active carbon chemical property is stable and there is good catalysis to live
Property；Stainless (steel) wire is a kind of basic structural materials of high intensity and has good conductive property that reticular structure can make sky
Gas penetrates；It can guarantee the pure culture environment of reactor in cathode outer side setting degerming filter membrane.It can guarantee using carbon paper as anode
The quantitative extraction of nucleic acid and protein sample, convenient for subsequent high-flux sequence and group credit analysis.
The single-chamber microbial fuel cell that the present invention constructs, under conditions of using sodium acetate as carbon source, access wild type and
Saltant, the steady output voltage period was up to 40 days or more.Agarose is carried out after extracting anode DNA sample by kit
Gel electrophoresis, band is limpid in sight, and reach group credit analysis builds library standard.
Detailed description of the invention
Fig. 1 is voltage output of the single-chamber microbial fuel cell access different strains of the present invention under pure culture.
Fig. 2 is the electrophoretogram after anode DNA sample of the present invention is extracted.
Fig. 3 is the structural schematic diagram of single-chamber microbial fuel cell reactor of the present invention；1- shell of reactor, 2- anode,
3- cathode, 4- degerming filter membrane, 5- conducting wire, 6- resistance, 7- titanium silk.
Specific embodiment
Embodiment 1 learns the single-chamber microbial fuel cell building of research for group
The single-chamber microbial fuel cell of research is learned by carbon paper anode, air cathode and glass for group in present embodiment
The integral structure that bottle body is constituted, the air cathode is by powdered capacitor activated carbon, conductive black, stainless (steel) wire and polytetrafluoro
Made of vac emulsion, degerming filter membrane is set on the outside of the air cathode.The preparation method of above-mentioned fuel cell is by following steps
Suddenly it carries out:
One, the powdered capacitor activated carbon of 10g is mixed under conditions of ultrasonic agitation with 63ml dehydrated alcohol
30min, ultrasonic agitation are slowly added to the ptfe emulsion that 1.333ml solid content is 60% in the process；It is steamed to dehydrated alcohol
When being sent to mixture formation micelle, with the continuous roll-in of roll squeezer until being pressed into the thin slice of 0.5mm to get Catalytic Layer.
Two, it carries out the powdered carbon black of 6g to mix 20min under conditions of ultrasonic agitation with 120ml dehydrated alcohol, ultrasound
The ptfe emulsion that 12ml solid content is 60%wt is slowly added in whipping process；Mixture shape is evaporated to dehydrated alcohol
It is then the stainless (steel) wire of 80 mesh with mesh mesh number with the continuous roll-in of roll squeezer until being pressed into the thin slice of 0.5mm when at micelle
(thickness about 0.5mm) is put into roll squeezer compacting together makes it be embedded in stainless (steel) wire, and mixing thin slice is put into 350 DEG C of Muffle furnace
Heat 20min.
Three, Catalytic Layer made from step 1 is stacked together with thin slice is mixed into roll squeezer, suppresses three to four times and obtains
The air cathode of 0.5mm.
Four, carbon paper is cut into after 2.5 × 8cm strip and is perforated with " J " type titanium silk of diameter 0.5mm, junction is smeared and led
Electric glue is fixed, and carbon paper anode is made.
Five, carbon paper anode is fixed in the reactor that dischargeable capacity is 250mL, titanium silk stretches out reactor head.It will be empty
Gas cathode is placed in reactor side, and Catalytic Layer is inside, and 0.22 μm of degerming filter membrane is covered outside diffusion layer, and loading makes at air cathode
It is clamping fixed with single flange and stainless steel.Electric current is exported with one section of titanium silk at cathode, external circuit is with copper wires by anode, 1000
Ω resistance is connected with cathode, completes the assembling of single-chamber microbial fuel cell reactor.
Using:
The single-chamber microbial fuel cell that the present embodiment is obtained, it is whole carry out high pressure steam sterilization after (121 DEG C,
20min), it is inoculated with 5mL bacterium solution, using the sodium acetate of 1g/L as substrate, using 100mM phosphate buffer as solvent, using interval
The mode of formula discontinuous flow is run in 35 DEG C of thermostatic chambers, under conditions of using sodium acetate as carbon source, accesses wild type and mutation
Type bacterial strain, the steady output voltage period was up to 40 days or more.Ago-Gel is carried out after extracting anode DNA sample by kit
Electrophoresis, band is limpid in sight, and reach group credit analysis builds library standard.
Claims (10)
1. a kind of single-chamber microbial fuel cell for the research of electro-chemical activity bacterium group, it is characterised in that: including reaction
Device shell (1), carbon paper anode (2), air cathode (3), degerming filter membrane (4), conducting wire (5), resistance (6), titanium silk (7), wherein anti-
Answering device shell is the glass body for having upper cover, side wall to have circular open, and the air cathode is by powdered capacitor activated carbon, leads
Made of electric carbon black, stainless (steel) wire and ptfe emulsion, the carbon paper anode is set to inside shell of reactor, with titanium silk
Connection, the upper cover that titanium silk passes through shell of reactor stretch out shell of reactor, connect resistance by conducting wire, resistance is connected by conducting wire
Another section of titanium silk, the titanium silk other end are connected with air cathode；The circle of shell of reactor side wall is arranged in the air cathode
Opening is fixed on shell of reactor by single flange and stainless steel folder, which is completely enclosed, set on the outside of air cathode
It is equipped with degerming filter membrane.
2. the single-chamber microbial fuel cell according to claim 1 for the research of electro-chemical activity bacterium group, special
Sign is: the anode carbon paper size is (2~2.5cm) × (6~10cm).
3. the single-chamber microbial fuel cell according to claim 1 for the research of electro-chemical activity bacterium group, special
Sign is: the glass body dischargeable capacity is 200~250ml, and glass bottle opening is sealed into upper cover, bottle body one using rubber rubber plug
Side circular open area is 6~8cm2。
4. the single-chamber microbial fuel cell according to claim 1 for the research of electro-chemical activity bacterium group, special
Sign is: the degerming filter sizes are 0.22 μm, and area is 8~10cm2。
5. a kind of described in any item single-chamber microbial fuel electricity for the research of electro-chemical activity bacterium group of claim 1-4
The preparation method in pond, it is characterised in that: carry out in the steps below:
Step 1: being stirred by ultrasonic after powdered capacitor activated carbon powder is mixed with dehydrated alcohol, it is slowly added to polytetrafluoroethylene (PTFE) cream
Liquid is evaporated to mixture to dehydrated alcohol and forms micelle, then with the continuous roll-in of roll squeezer until compacting flakiness, is made air
The Catalytic Layer of cathode；
Step 2: being stirred by ultrasonic after conductive black is mixed with dehydrated alcohol, it is slowly added to ptfe emulsion, to anhydrous second
Alcohol is evaporated to mixture and forms micelle, with the continuous roll-in of roll squeezer until compacting flakiness, is then put into stainless (steel) wire together
Roll squeezer compacting makes it be embedded in stainless (steel) wire, and mixing thin slice is put into Muffle furnace and is heated；
Step 3: the thin slice after heating that Catalytic Layer made from step 1 is prepared with step 2 is superimposed placement, it is put into roller together
Press is suppressed three to four times and obtains air cathode；
Step 4: being perforated after carbon paper is cut with titanium silk, and smear conducting resinl and fix, carbon paper anode is made；
Step 5: air cathode, degerming filter membrane, carbon paper anode and glass body are assembled integrally structure, the micro- life of single chamber is made
Object fuel cell.
6. the preparation of the single-chamber microbial fuel cell according to claim 5 for the research of electro-chemical activity bacterium group
Method, it is characterised in that: the mass ratio of capacitor activated carbon powder and dehydrated alcohol is 1:(4~6 in step 1), the time is stirred by ultrasonic
For 20~40min, Catalytic Layer is with a thickness of 0.4~0.6mm.
7. the preparation of the single-chamber microbial fuel cell according to claim 5 for the research of electro-chemical activity bacterium group
Method, it is characterised in that: ptfe emulsion solid content is 40%~80%wt, capacitor activated carbon powder and poly- four in step 1
Vinyl fluoride quality of the emulsion ratio is (4~6): 1.
8. the preparation of the single-chamber microbial fuel cell according to claim 5 for the research of electro-chemical activity bacterium group
Method, it is characterised in that: conductive black and dehydrated alcohol mass ratio are 1:(2~3 in step 2), the ultrasonic agitation time is 15~
30min, sheet thickness are 0.4~0.6mm.
9. the preparation of the single-chamber microbial fuel cell according to claim 5 for the research of electro-chemical activity bacterium group
Method, it is characterised in that: ptfe emulsion solid content is 40%~80%wt, conductive black and polytetrafluoroethyl-ne in step 2
Alkene quality of the emulsion ratio is (1~1.2): 3；The mesh number of stainless (steel) wire is 40~100 mesh.
10. the system of the single-chamber microbial fuel cell according to claim 5 for the research of electro-chemical activity bacterium group
Preparation Method, it is characterised in that: be assembled integrally air cathode, degerming filter membrane, carbon paper anode and glass body described in step 5
Structure, assemble according to following connection relationship: shell of reactor is the glass body for having upper cover, side wall to have circular open, the sky
Gas cathode is the carbon paper sun made of powdered capacitor activated carbon, conductive black, stainless (steel) wire and ptfe emulsion
Pole is set to inside shell of reactor, is connect with titanium silk, and the upper cover that titanium silk passes through shell of reactor stretches out shell of reactor, is passed through
Conducting wire connects resistance, and resistance connects another section of titanium silk by conducting wire, which is connected with air cathode；The air yin
Pole is arranged at the circular open of shell of reactor side wall, is fixed on shell of reactor by single flange and stainless steel folder, will
The opening is completely enclosed, is provided with degerming filter membrane on the outside of air cathode.
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| CN103367784A (en) * | 2013-07-28 | 2013-10-23 | 桂林理工大学 | Single-chamber no-film air-cathode microbiological fuel cell device |
| CN108767265A (en) * | 2018-06-19 | 2018-11-06 | 哈尔滨工业大学 | A kind of single-chamber microbial fuel cell activated carbon-film-air cathode and preparation method thereof |
| CN109378508A (en) * | 2018-09-30 | 2019-02-22 | 天津大学 | A kind of single-chamber microbial fuel cell and its application method adding degradation class bacterium |
-
2019
- 2019-08-30 CN CN201910814486.5A patent/CN110518273B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101237063A (en) * | 2007-12-19 | 2008-08-06 | 华南理工大学 | Self-medium coupled microbe fuel battery for single room micro filtering |
| US20100093078A1 (en) * | 2008-10-14 | 2010-04-15 | Cleveland State University | Separating device, an algae culture photobioreactor, and methods of using them |
| CN101702440A (en) * | 2009-10-29 | 2010-05-05 | 浙江大学 | Single-chamber microbial fuel cell without metal catalyst |
| CN103367784A (en) * | 2013-07-28 | 2013-10-23 | 桂林理工大学 | Single-chamber no-film air-cathode microbiological fuel cell device |
| CN108767265A (en) * | 2018-06-19 | 2018-11-06 | 哈尔滨工业大学 | A kind of single-chamber microbial fuel cell activated carbon-film-air cathode and preparation method thereof |
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