CN110511135A - A method of adsorbed using chitosan-separate shikimic acid - Google Patents
A method of adsorbed using chitosan-separate shikimic acid Download PDFInfo
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- CN110511135A CN110511135A CN201910913381.5A CN201910913381A CN110511135A CN 110511135 A CN110511135 A CN 110511135A CN 201910913381 A CN201910913381 A CN 201910913381A CN 110511135 A CN110511135 A CN 110511135A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/14—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the introduction of the feed to the apparatus
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
- B01D15/166—Fluid composition conditioning, e.g. gradient
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/24—Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
- C07C51/47—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C2601/00—Systems containing only non-condensed rings
- C07C2601/12—Systems containing only non-condensed rings with a six-membered ring
- C07C2601/16—Systems containing only non-condensed rings with a six-membered ring the ring being unsaturated
Abstract
The invention discloses a kind of adsorptive-separation techniques of shikimic acid, belong to plant purification technique field.It is specifically implemented according to the following steps: preparing shikimic acid extracting solution from the natural plants containing shikimic acid；By shikimic acid extracting solution ultrafiltration, and after cationic resin column is handled, shikimic acid upper prop solution is obtained；Shikimic acid upper prop solution continuous flow shell adding glycan adsorption column is adsorbed, then washed with the ethanol solution of 40-80%, the sodium hydroxide solution elution containing ethyl alcohol 40~80% for being again 8~12 with pH value, obtain shikimic acid eluent, it is handled again through cationic resin column, and be concentrated, dry, obtain shikimic acid.Present invention utilizes chitosans in the thick grass acid solution containing 60% ethyl alcohol in non-swelling situation, shikimic acid effectively in absorption natural plants, pass through the shikimic acid of the caustic washing shelling glycan absorption containing ethyl alcohol, to realize the absorption of shikimic acid with separate, the final purity that obtains is 72~82% shikimic acid.
Description
Technical field
The invention belongs to plant purification technique fields, and in particular to a kind of side for being adsorbed-being separated shikimic acid using chitosan
Method.
Background technique
Shikimic acid (Shikimic Acid, 3R, 4S, 5R-E-3,4,5-Trihydroxy-l-
Cyclohexenecarboxylic Acid, CARSN 138-59-0) it is a cyclol derivant, a kind of chirality of single configuration
Compound.Shikimic acid has anti-oxidant, antibacterial, antitumor, anti-inflammatory, antithrombotic, inhibits the important lifes such as cranial nerve cell apoptosis
Object activity, mainly applies the following aspects: (1) developing antiviral drugs.Shikimic acid is the antiviral drugs for treating bird flu
The intermediate of " Tamiflu " main component Oseltamivir phosphate.(2) antibacterial anti-tumor agent is developed.Utilize methyl shikimate synthesis
Aldoketonutase inhibitor analogue has apparent inhibiting effect to hela cell strain and Ai Xili ascites carcinoma.(3) exploitation is cardiovascular
Disease preparation.Shikimic acid has significant anti thrombotic action, can inhibit the formation of artery and vein thrombus and cerebral thrombosis.(4) it develops anti-inflammatory
Drug.Shikimic acid can inhibit the expression of inflammatory factor, play inhibiting effect to inflammatory reaction.
Currently, although shikimic acid can be chemically synthesized method or the preparation that undergoes microbial fermentation, it is main or from
It extracts and obtains in natural plants.The industrialized production of shikimic acid is mainly extracted from Chinese anise in the prior art, but due to
Chinese anise raw material supply is limited, expensive and production technology is complicated, productivity is low.In addition a large amount of harmful influence is used,
Serious environmental pollution is caused, leading to market shikimic acid, supply falls short of demand, therefore finds the extraction raw material gesture of new shikimic acid must
Row.
Chitosan (chitosan) is that the chitin (chitin) being widely present by nature is obtained by deacetylation
It arrives, is the polymer of Glucosamine.The each monomer of chitosan (Glucosamine) has a free amino, chitosan
It is not soluble in water, but since free amino can be acted on sour (inorganic acid or organic acid) into salt, therefore have suction-operated to acid, due to
The molecular weight of each monomer (Glucosamine) is close with most organic acids, therefore chitosan has very strong adsorption energy to organic acid
Power.But chitosan is easy to be swollen in acidic aqueous solution and forms colloidal compound, makes acidic materials (organic acid or the nothing of absorption
Machine acid) it is difficult to separate, chitosan is also difficult to regenerate, to limit its application in acidic materials separation.Therefore, of the invention
It aims to solve the problem that the problem of chitosan is swollen in acidic aqueous solution and forms colloidal compound, and chitosan is used for organic acid and is inhaled
It is attached, a kind of extracting method of higher degree shikimic acid is developed, provides technological approaches to prepare high-pure shikimic acid.
Summary of the invention
The present invention provides a kind of method for adsorbing-separating shikimic acid using chitosan, chitosan adsorbs-separate shikimic acid
When, swelling does not form colloidal cpd to chitosan, makes it possible absorption-separation of organic acid and the regeneration of chitosan.
First purpose of the invention is to provide a kind of method for adsorbing-separating shikimic acid using chitosan, specifically according to
Lower step is implemented:
Step 1, shikimic acid extracting solution is prepared from the natural plants containing shikimic acid；
Step 2, shikimic acid extracting solution is handled with cationic resin column, obtains pretreatment fluid；Pretreatment fluid is added to
In 95% ethanol solution of volumetric concentration, shikimic acid upper prop solution is obtained；Wherein, the volume ratio of pretreatment fluid and ethanol solution is 37
~42%:58-63%；
Step 3, chitosan adsorption column is added in shikimic acid upper prop solution, when efflux pH value is 4.5~5.5, uses body
The ethanol solution of product concentration 40-80% washs chitosan adsorption column, is 8~12 with pH value when washing to efflux is neutral
The sodium hydroxide solution elution containing ethyl alcohol be adsorbed with the chitosan adsorption column of shikimic acid, and collect eluent, as shikimic acid
Eluent；Wherein, ethyl alcohol of the total volume 40~80% in the sodium hydroxide solution containing ethyl alcohol；
Step 4, shikimic acid eluent is handled with cationic resin column again, obtains purification process liquid, by purification process liquid
Concentration is dried to get shikimic acid is arrived.
Preferably, the natural plants containing shikimic acid are Chinese anise, pine needle or ginkgo leaf.
Preferably, when the natural plants containing shikimic acid are that Chinese anise prepares shikimic acid extracting solution, including following step
It is rapid: it takes Chinese anise to crush sieving, after extracting fennel oil by distillation, surplus solution after distillation is taken to be cooled down, be concentrated, it is fixed
Hold, obtains shikimic acid extracting solution；Wherein, the surplus solution after every 100g Chinese anise distillation should be settled to 500mL；
When the natural plants containing shikimic acid are that pine needle prepares shikimic acid extracting solution, comprising the following steps: take pine needle meal
It is broken, it is filtered after being heated to reflux by addition distilled water, takes filtrate, obtain shikimic acid extracting solution；Wherein, the pine needle of every 300g needs
The distilled water of 7500mL is heated to reflux；
When the natural plants containing shikimic acid are that ginkgo leaf prepares shikimic acid extracting solution, comprising the following steps: take ginkgo
Leaf is filtered after being heated to reflux by the ethanol solution that volumetric concentration is 70%, and distilled water centrifugation is added after concentration,
It takes on supernatant after macroporous resin column, takes filtrate, obtain shikimic acid extracting solution, wherein the amount of distilled water is added, is by every 1kg
The ginkgo leaf amount of investment tank body adds 3L distilled water.
Preferably, in step 2 and step 4, the cationic resin column is LX-04 ion exchange resin column.
Preferably, the chitosan adsorption column the preparation method is as follows: take deacetylation be 82-92%, granularity 40-100
Purpose natural cationic type polymer shell glycan, the ethanol solution for being 40-95% with volumetric concentration is wet, then fills column, obtains
Chitosan adsorption column.
Preferably, in step 2, before shikimic acid extracting solution is handled with cationic resin column, using the ultrafiltration membrane pair of 200KDa
Shikimic acid extracting solution ultrafiltration.
Compared with prior art, the beneficial effects of the present invention are:
It 1) is 82-92% present invention utilizes deacetylation, the natural cationic type polymer shell of granularity 40-100 mesh is poly-
Adsorbent of the sugar as shikimic acid makes organic in order to avoid chitosan in the presence of having water and organic acid form colloidal cpd
The separation of acid is difficult, is by volume the ethyl alcohol that 37~42%:58-63% is added to 95% by the pretreatment fluid containing shikimic acid
In, chitosan not swelling during adsorbing shikimic acid can be made, it is ensured that shikimic acid is effectively adsorbed on chitosan；
2) present invention by shikimic acid upper prop solution be added chitosan adsorption column, when detect efflux pH value be 4.5~5.5
When, wash chitosan adsorption column with the ethanol solution of volumetric concentration 40-80%, when efflux is detected as neutrality until, effectively
Remove other impurities；Eluent is finally eluted and collects with the sodium hydroxide solution containing ethyl alcohol that pH value is 8~12, to realize
The absorption of shikimic acid with separate.In addition, in order to avoid chitosan swelling, cleaning solution and eluent in washing and elution process
Middle ethyl alcohol accounts for the 40~80% of volume；Meanwhile convenient for the regeneration of the chitosan adsorption column after elution.
The present invention by LX-04 cation exchange resin column to shikimic acid extracting solution processing, can effectively remove metal from
The cationic substances such as son and protein keep shikimic acid completely free；It is received in the sodium hydroxide solution elution process containing ethyl alcohol
Collection eluent passes through LX-04 cation exchange resin column again and handles, and effectively removes sodium ion, and obtaining purity is 72~82%
Shikimic acid.
Chitosan of the present invention adsorb-separate shikimic acid method can in fermentation liquid, industrial wastes other organic acids
It is applied well in recycling.
Specific embodiment
In order to enable those skilled in the art to more fully understand, technical solution of the present invention is practiced, below with reference to specific
Embodiment is next, and the invention will be further described, but illustrated embodiment is not as a limitation of the invention.
Experimental method described in following each embodiments is unless otherwise specified conventional method；Material therefor is such as without special
Illustrate, can be commercially available on the market.
Embodiment 1
A method of adsorbed using chitosan-shikimic acid is separated, it is specifically implemented according to the following steps:
Step 1, shikimic acid extracting solution is prepared from Chinese anise: the Chinese anise for taking 100g dry crushed 60 meshes,
It is packed into distilling flask, is heated after distilled water is added, steam distillation extracts fennel oil, after fennel oil recycling completely, cucurbit
In aqueous extract it is cooling after 200KDa ultrafiltration membrane ultrafiltration, concentration, constant volume to 500ml, acquisition shikimic acid extracting solution；
Step 2, shikimic acid extracting solution is obtained pretreatment fluid, will pre-processed after the processing of LX-04 ion exchange resin column
Liquid is added in 95% ethanol solution of volumetric concentration, obtains shikimic acid upper prop solution；Wherein, the body of pretreatment fluid and ethanol solution
Product is than being 37:63；HPLC detects shikimic acid upper prop solution, and thick grass acid concentration is 9.1mg/mL；
Step 3, the flow velocity continuous flow shell adding glycan adsorption column that shikimic acid upper prop solution is pressed to 5ml/min, as efflux pH
Value is 4.5, washs chitosan adsorption column with the ethanol solution of volumetric concentration 40%, is 8 with pH value when efflux is neutral
Sodium hydroxide solution elution containing ethyl alcohol is adsorbed with the chitosan adsorption column of shikimic acid, and collects eluent, as thick grass pickling
De- liquid；
Wherein, ethyl alcohol of the total volume 40% in the sodium hydroxide solution containing ethyl alcohol；Chitosan adsorption column is using deacetylated
Degree is 82%, and the natural cationic type polymer shell glycan of 40 mesh of granularity is wet by 40% ethanol water, then fills column and obtains
.
Step 4, it uses LX-04 cationic resin column to handle again shikimic acid eluent, obtains purification process liquid, examined through HPLC
It surveys, thick grass acid content is 8.2mg/ml, yield 90%；It is finally 75% to get purity is arrived by the concentration of purification process liquid, drying
Shikimic acid.
Embodiment 2
A method of adsorbed using chitosan-shikimic acid is separated, it is specifically implemented according to the following steps:
Step 1, shikimic acid extracting solution is prepared from pine needle: the fresh Korean Needle of 300g being taken to crush, and 4500mL distillation is added
Water is heated to reflux 2h, and filtering continuously adds 3000mL distilled water and is heated to reflux 2h, filters, and merges filtrate twice, obtains shikimic acid
Extracting solution；
Step 2, by ultrafiltration membrane ultrafiltration of the shikimic acid extracting solution using 200KDa, and through LX-04 ion exchange resin column
After processing, concentration is settled to 1000ml, obtains pretreatment fluid, pretreatment fluid is added in 95% ethanol solution of volumetric concentration,
Obtain shikimic acid upper prop solution；Wherein, the volume ratio of pretreatment fluid and ethanol solution is 42:58；HPLC detects shikimic acid upper prop
Solution, thick grass acid concentration are 8.8mg/mL；
Step 3, the flow velocity continuous flow shell adding glycan adsorption column that shikimic acid upper prop solution is pressed to 5ml/min, as efflux pH
Value is 5.5, washs chitosan adsorption column with the ethanol solution of volumetric concentration 80%, is 12 with pH value when efflux is neutral
The sodium hydroxide solution elution containing ethyl alcohol be adsorbed with the chitosan adsorption column of shikimic acid, and collect eluent, as shikimic acid
Eluent；
Wherein, ethyl alcohol volume accounts for the 80% of volume in the sodium hydroxide solution containing ethyl alcohol；Chitosan adsorption column is using de- second
Acyl degree is 95%, and the natural cationic type polymer shell glycan of 100 mesh of granularity is wet by 95% ethanol water, is then filled
Column obtains.
Step 4, it uses LX-04 cationic resin column to handle again shikimic acid eluent, obtains purification process liquid, examined through HPLC
It surveys, thick grass acid content is 7.7mg/ml, and the concentration of purification process liquid, drying are finally 72% to get purity is arrived by yield 82%
Shikimic acid.
Embodiment 3
A method of adsorbed using chitosan-shikimic acid is separated, it is specifically implemented according to the following steps:
Step 1, shikimic acid extracting solution is prepared from ginkgo leaf: ginkgo leaf being taken to put into extractor, and 8 times of ginkgo leaf amount are added
The ethanol solution that volumetric concentration is 70%, is heated to reflux 2h, filters, and continues 6 times of volumes that ginkgo leaf amount is added in extractor
The ethanol solution that concentration is 70%, is heated to reflux 2h, merges secondary ethanol extract, and ethyl alcohol is removed in concentration, obtains concentrate；Then
Distilled water centrifugation is added into concentrate, takes macroporous resin column on supernatant to be adsorbed and removed flavonol glycosides and lactone, takes filter
Liquid obtains shikimic acid extracting solution, wherein the amount of distilled water is added, is to add 3L to distill by the ginkgo leaf amount of every 1kg investment tank body
Water；
Step 2, by shikimic acid extracting solution use 200KDa ultrafiltration membrane ultrafiltration, and through LX-04 ion exchange resin column at
After reason, pretreatment fluid is obtained, takes 1200ml pretreatment fluid to be added in 95% ethanol solution of volumetric concentration, obtains shikimic acid upper prop
Solution；Wherein, the volume ratio of pretreatment fluid and ethanol solution is 40:60；HPLC detects shikimic acid upper prop solution, thick grass acid concentration
For 9.1mg/mL；
Step 3, the flow velocity continuous flow shell adding glycan adsorption column that shikimic acid upper prop solution is pressed to 5ml/min is flowed when detecting
Liquid pH value is 5.0 out, washs chitosan adsorption column with the ethanol solution of volumetric concentration 60%, when efflux is neutral, uses pH
The sodium hydroxide solution elution containing ethyl alcohol that value is 10 is adsorbed with the chitosan adsorption column of shikimic acid, and collects eluent, as
Shikimic acid eluent；
Wherein, ethyl alcohol accounts for the 60% of volume in the sodium hydroxide solution containing ethyl alcohol；Chitosan adsorption column uses deacetylation
It is 85%, the natural cationic type polymer shell glycan of 50 mesh of granularity is wet by 60% ethanol water, then fills column and obtains
.
Step 4, it uses LX-04 cationic resin column to handle again shikimic acid eluent, obtains purification process liquid, examined through HPLC
It surveys, thick grass acid content is 8.5mg/ml, yield 93%.It is finally 82% to get purity is arrived by the concentration of purification process liquid, drying
Shikimic acid.
In addition, washing adsorption column extremely with 70% ethanol solution after the completion of adsorption column elutes in above-described embodiment 1~3
Efflux is in neutrality, and completes absorption column regeneration, reusable.
Comparative example 1
The test of ethyl alcohol is not added: the chitosan addition pure water of 100 mesh deacetylation 88% of 10g dilutes one times of thick grass
Acid solution 100ml (contain shikimic acid 840mg), at room temperature glass bar stirring chitosan glue dough, pH be in neutrality, Wu Fajin
The processing of one step.
It can be swollen and be formed in acidic aqueous solution as natural cationic type polymer in order to further illustrate chitosan
Colloidal compound makes the operating difficulties of the acidic materials (organic acid or inorganic acid) of absorption, and acidic materials are difficult to separate, the present invention
Thick grass acid solution is mixed with ethyl alcohol, the variation of chitosan volume when comparing different ethanol concentration, discovery contains in thick grass acid solution
Chitosan is almost non-swelling when concentration of alcohol 60% or so, and realizes absorption and separation of the chitosan to shikimic acid.Specific implementation
It is as follows:
One, chitosan equilibrium swelling experiments are specific as follows:
The centrifuge tube of 6 10ml is taken, the chitosan powder of 0.5g (± 0.0005g) deacetylation 88% is accurately added in every pipe
(100 mesh), being carefully added into alcohol volume content after label respectively is 10%, 20%, 30%, 40%, 60%, 80% shikimic acid
Solution 10ml (is handled after thick grass acid solution ultrafiltration with resin cation), and it is control that 95% ethyl alcohol 10ml, which is added, in another pipe, is stood
4000r/min is centrifuged 5min after 1h, takes out test tube, measures the deposition height of centrifuge tube bottom chitosan, is shown in Table 1.
The influence that thick grass acid solution of the table 1 containing different concentration ethanol is swollen chitosan
As it can be seen from table 1 alcohol volume content chitosan at 60% or so is almost non-swelling in thick grass acid solution,
It sufficiently proves the technical scheme is that feasible, and excellent effect.
Two, chitosan is specifically implemented according to the following steps the adsorption rate of shikimic acid:
The chitosan 3.0g (± 0.0005g) of 60 mesh deacetylations 88% is taken, 200ml dehydrated alcohol is added and 100ml is super
The thick grass acid solution of filter and cationic resin column processing, magnetic stirrer 4h, takes supernatant HPLC to detect thick grass at room temperature
The concentration of acid, every gram of chitosan can adsorb 0.16g shikimic acid, i.e. adsorption rate is 16%.
The present invention can also be used in fermentation liquid, in industrial wastes other organic acids recycling.
It should be noted that the present invention relates to when numberical range, it is thus understood that two endpoints of each numberical range and
Any one numerical value can be selected between two endpoints, since the step method of use is identical as embodiment 1-3, go to live in the household of one's in-laws on getting married in order to prevent
It states, the present invention describes preferred embodiment, and once a person skilled in the art knows basic creative concepts, then may be used
These embodiments are made with other change and modification.So the following claims are intended to be interpreted as include preferred embodiment with
And fall into all change and modification of the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
Mind and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (6)
1. a kind of method for adsorbing-separating shikimic acid using chitosan, which is characterized in that be specifically implemented according to the following steps:
Step 1, shikimic acid extracting solution is prepared from the natural plants containing shikimic acid；
Step 2, shikimic acid extracting solution is handled with cationic resin column, obtains pretreatment fluid；Pretreatment fluid is added to volume
In 95% ethanol solution of concentration, shikimic acid upper prop solution is obtained；Wherein, the volume ratio of pretreatment fluid and ethanol solution be 37~
42%:58-63%；
Step 3, chitosan adsorption column is added in shikimic acid upper prop solution, it is dense with volume when efflux pH value is 4.5~5.5
The ethanol solution for spending 40-80% washs chitosan adsorption column, is 8~12 to contain with pH value when washing to efflux is neutral
The sodium hydroxide solution elution of ethyl alcohol is adsorbed with the chitosan adsorption column of shikimic acid, and collects eluent, and as shikimic acid elutes
Liquid；Wherein, ethyl alcohol of the total volume 40~80% in the sodium hydroxide solution containing ethyl alcohol；
Step 4, shikimic acid eluent is handled with cationic resin column again, obtains purification process liquid, purification process liquid is concentrated,
It dries to get shikimic acid is arrived.
2. the method according to claim 1 for adsorbing-separating shikimic acid using chitosan, which is characterized in that described containing big
The natural plants of oxalic acid are Chinese anise, pine needle or ginkgo leaf.
3. the method according to claim 2 for adsorbing-separating shikimic acid using chitosan, which is characterized in that when with illiciumverum
When fennel prepares shikimic acid extracting solution, comprising the following steps: Chinese anise extracts fennel oil, extraction raffinate water after distillation by distillation
Constant volume obtains shikimic acid extracting solution；Wherein, the amount ratio of the shikimic acid extracting solution obtained after Chinese anise and constant volume is 100g:
500mL；
When preparing shikimic acid extracting solution with pine needle, comprising the following steps: pine needle distilled water refluxing extraction obtains shikimic acid extraction
Liquid；Wherein, the ratio between dosage of pine needle and distilled water is 300g:7500ml；
When preparing shikimic acid extracting solution with ginkgo leaf, comprising the following steps: after ginkgo leaf is extracted with alcohol reflux, filter dense
Contracting, concentrate are added distilled water centrifugation, take supernatant, then by macroporous resin treatment, obtain shikimic acid extracting solution,
In, the amount ratio of ginkgo leaf and distilled water is 1kg:3L.
4. the method according to claim 1 for adsorbing-separating shikimic acid using chitosan, which is characterized in that step 2 and step
In rapid 4, the cationic resin column is LX-04 ion exchange resin column.
5. the method according to claim 1 for adsorbing-separating shikimic acid using chitosan, which is characterized in that the shell is poly-
Sugared adsorption column the preparation method is as follows: take deacetylation be 82-92%, granularity 40-100 mesh natural cationic type polymer shell
Glycan, the ethanol solution for being 40-95% with volumetric concentration is wet, then fills column, obtains chitosan adsorption column.
6. the method according to claim 1 for adsorbing-separating shikimic acid using chitosan, which is characterized in that in step 2,
Before shikimic acid extracting solution is handled with cationic resin column, using the ultrafiltration membrane of 200KDa to shikimic acid extracting solution ultrafiltration.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111056941A (en) * | 2019-12-26 | 2020-04-24 | 浙江康恩贝制药股份有限公司 | Method for preparing high-purity shikimic acid by utilizing ginkgo leaf extract chromatography waste liquid |
| CN112704074A (en) * | 2021-01-05 | 2021-04-27 | 广东省田园农业生物科技有限公司 | Method for extracting natural plant endogenous components |
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| CN112704074A (en) * | 2021-01-05 | 2021-04-27 | 广东省田园农业生物科技有限公司 | Method for extracting natural plant endogenous components |
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