CN110507753A - A kind of traditional Chinese medicine extract composition and its preparation method and application for treating cognitive disorder disease - Google Patents
A kind of traditional Chinese medicine extract composition and its preparation method and application for treating cognitive disorder disease Download PDFInfo
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Abstract
The present invention relates to a kind of traditional Chinese medicine extract compositions for treating cognitive disorder disease, the traditional Chinese medicine extract composition is made of the anemarrhena asphodefoides extract containing anemarrhena total saponin and the Bulbus Lilii extract containing lilium total saponin, and the weight ratio of anemarrhena total saponin, lilium total saponin is 1:2.The invention also includes the preparation methods of the traditional Chinese medicine extract composition and its application in the drug of preparation treatment cognitive disorder disease.Traditional Chinese medicine extract composition of the invention shows rhizoma anemarrhenae through pharmacodynamic test, the composition of Bulbus Lilii extract plays the role of significant anti-cognitive disorder.The high and of the invention traditional Chinese medicine extract composition collaboration of each saponin content that preparation method of the invention obtains proves effective, and drug action is clear, and quality control is rigorous, taking dose is small, convenient to take.
Description
Technical field
The present invention relates to medical pharmaceutical technology fields, specifically, being a kind of traditional Chinese medicine extraction for treating cognitive disorder disease
Compositions and its preparation method and application.
Background technique
Cognition be body understanding and obtain knowledge Intelligent Machining process, be related to study, memory, language, thinking, spirit,
A series of random, psychology such as emotion and social action.Cognitive disorder refer to judge with above-mentioned learning and memory and thinking it is related big
There is exception in brain high-grade intelligent process, so as to cause serious study, memory disorders, while with aphasia or appraxia or agnosia
Or lose the pathologic process of the changes such as row.The basis of cognition is corticocerebral normal function, it is any cause cerebral cortex function and
The factor of textural anomaly can lead to cognitive disorder.Since the function of brain is complicated, and the different type cross-correlation of cognitive disorder
Connection, i.e., cognitive question in a certain respect can cause on the other hand or the cognition of many aspects is abnormal (if for example, a patient has
The defect of attention and memory aspect, with regard to will appear the obstacle solved the problems, such as).Its clinical manifestation includes:
1. disturbance of perception, such as hyperesthesia, insensitive, cenesthopathy, feel rotten, sensory deprivation, pathological illusion,
Illusion, disturbance of perceptive synthesis；
2. memory disorders such as remember too strong, memory defects, promnesia；
3. the disturbance of thought, such as abstract process obstacle, associative process obstacle, thinking logic obstacle, vain hope.
The reason of above-mentioned various cognitive disorders be it is diversified, in addition to organic disease reason, most mental disease institute
It causes.Such as neurasthenia, hysteria, doubt disease, menopausal syndrome, depression, obsessive-compulsive disorder, senile dementia, schizophrenia, reaction
Property mental disease, Paranoid psychosis, mania, anxiety disorder etc..
Include: for the therapeutic scheme of the disease at present
1. suit the medicine to the illness and neuroprotective therapy, and, for the cause of disease and pathogenesis of cognitive disorder, different minds can be applied
Through cell-protecting, such as Brain circlulation improver, energetic supersession activator, neurotransmitter and nerve growth factor protective agent, Ca2+
Antagonist, glutamate receptor antagonist, antioxidant, spongiocyte regulator and non-steroidal anti-inflammatory agent etc. are answered extensively
Treatment for cognitive disorder caused by various disease；
2. restoring and maintaining the normal level of neurotransmitter；
3. operative treatment.
Above-described therapeutic scheme belongs to western medical treatment scheme, and certain side effect can be generated to patient.
About Chinese traditional treatment: rhizoma anemarrhenae lily medicine is to " Synopsis Golden Chamber " first recorded in Eastern Han Dynasty's Zhang Zhongjing, by lily and rhizoma anemarrhenae two
Taste Chinese medicinal composition, " hundred arteries and veins are all towards in lung.It is real that lily can control perverse trend, and the void of reinforcing vital qi；Rhizoma anemarrhenae enters lung gold, its beneficial water source, under
Logical bladder, closes the gas of the sky and water, and the yin hurt turns, then its heresy goes out from urine." two medicine compatibilities, a profit one is clear, and one mends one
Rush down, play enriching yin altogether and dispel heat, disappear it is tired moisturize, the benefits of profit heart supports lung.Lily is the dry of liliaceous plant tiger lily, lily or Lilium tenuifolium
Dry fleshy scale leaf.It is sweet in flavor, it is cold in nature.Distributed in heart and lung channels.With Yin nourishing and lung moistening, the effect of clearing away the heart fire and tranquillizing.For dry cough due to yin deficiency, overstrain cough
Hemoptysis, dysphoria palpitates with fear, insomnia and dreamful sleep are absent-minded.Rhizoma anemarrhenae is the dry rhizome of liliaceae plant.It is bitter, sweet, it is cold in nature.
Return lung, stomach, kidney channel.With clearing heat-fire, the effect of nourishing Yin and moistening dryness.For fever caused by exogenous pathogenic factors, hyperpyrexia polydipsia, lung-heat type cough, hectic fever due to yin
Hectic fever, Heat Diabetes, dry constipation of intestines.The warp of the spiritual neurogenic diseases such as traditional Chinese medicine treatment depression, insomnia, anxiety is become
Allusion quotation recipe.Studies have shown that rhizoma anemarrhenae Lilii Decoction can be by adjusting the growth of hippocampal neuron and being metabolized associated signal paths, to repair
The pathological change of multiple hippocampus of rats.
Traditional one auxiliary of lilii and Anemarrhenae Decoction agent is by 7 pieces of lily, and three liang of rhizoma anemarrhenae compositions, dosage is big, inconvenient to carry.
Inventor in view of the drawbacks of the prior art, devises a kind of Chinese medical extract for treating cognitive disorder disease of the present invention
Composition and its preparation method and application, about this content at present there is no literature reported on.
Summary of the invention
The first purpose of this invention is in view of the deficiencies of the prior art, to provide a kind of Chinese medicine for treating cognitive disorder disease
Extractive composition.
Second object of the present invention is in view of the deficiencies of the prior art, to provide traditional Chinese medicine extract composition as described above
Purposes.
Third object of the present invention is in view of the deficiencies of the prior art, to provide a kind of Chinese medicine for treating cognitive disorder disease
The preparation method of extractive composition.
Fourth object of the present invention is in view of the deficiencies of the prior art, to provide a kind of medicine for treating cognitive disorder disease
Object.
5th purpose of the invention is in view of the deficiencies of the prior art, to provide the preparation method of drug as described above.
To realize above-mentioned first purpose, the technical solution adopted by the present invention is that:
A kind of traditional Chinese medicine extract composition for treating cognitive disorder disease, which is characterized in that the Chinese medical extract group
It closes object to be made of the anemarrhena asphodefoides extract containing anemarrhena total saponin and the Bulbus Lilii extract containing lilium total saponin, the traditional Chinese medicine extraction
Anemarrhena total saponin in compositions: the weight ratio of lilium total saponin is 1:2；
The anemarrhena asphodefoides extract and Bulbus Lilii extract the preparation method is as follows:
(A) Bulbus Lilii extract:
By the drying 12 hours of 100 DEG C of lily medicinal material, until water content 10% is hereinafter, crushing, crosses 14 meshes, 3 times of 30% second of amount
Alcohol infiltrate 6h after, then use 30% ethanol percolation, diacolation speed be 1.2BV/h, collect 24 times of amount percolates, be concentrated under reduced pressure into density
For 1.0-1.5g/ml, cooling, filtering, filtrate is upper prop sample liquid, and upper prop sample liquid is passed through by 0.8BV applied sample amount and is equipped with
In the chromatographic column of resin, 95% ethyl alcohol of the distilled water of 12BV, 20% ethyl alcohol of 12BV, 70% ethyl alcohol of 10BV, 8BV are successively used
Gradient elution, flow velocity 2.5BV/h, 70% ethanol eluate are concentrated under reduced pressure, and it is that lily is total that drying, which simultaneously crushes to obtain pale yellow powder,
Saponin(e finished product；
(B) anemarrhena asphodefoides extract:
By the drying 12 hours of 100 DEG C of rhizoma ane marrhenae, until water content 10% is hereinafter, crushing, crosses 14 meshes, 3 times of 30% second of amount
Alcohol infiltrate 6h after, then use 30% ethanol percolation, diacolation speed be 1.2BV/h, collect 24 times of amount percolates, be concentrated under reduced pressure into density
For 1.0-1.5g/ml, cooling, filtering, filtrate is upper prop sample liquid.Upper prop sample liquid is passed through by 0.8BV applied sample amount and is equipped with
In the chromatographic column of resin, 95% ethyl alcohol of the distilled water of 12BV, 20% ethyl alcohol of 12BV, 70% ethyl alcohol of 10BV, 8BV are successively used
Gradient elution, flow velocity 2.5BV/h, 70% ethanol eluate are concentrated under reduced pressure, and it is that rhizoma anemarrhenae is total that drying, which simultaneously crushes to obtain pale yellow powder,
Saponin(e finished product.
As a preferred embodiment of the invention, lilium total saponin is flat in Bulbus Lilii extract made from step (A)
Equal content is 70%-100%；The content of anemarrhena total saponin is 70%-100% in anemarrhena asphodefoides extract made from step (B).
To realize above-mentioned second purpose, the technical solution adopted by the present invention is that:
As above application of any traditional Chinese medicine extract composition in the drug of preparation treatment cognitive disorder disease.
As a preferred embodiment of the invention, the cognitive disorder disease refers to phychasthnia, hysteria, depression
Disease, senile dementia, schizophrenia.
To realize above-mentioned third purpose, the technical solution adopted by the present invention is that:
A kind of preparation method of traditional Chinese medicine extract composition that treating cognitive disorder disease, includes the following steps:
(A) Bulbus Lilii extract is prepared:
By the drying 12 hours of 100 DEG C of lily medicinal material, until water content 10% is hereinafter, crushing, crosses 14 meshes, 3 times of 30% second of amount
Alcohol infiltrate 6h after, then use 30% ethanol percolation, diacolation speed be 1.2BV/h, collect 24 times of amount percolates, be concentrated under reduced pressure into density
For 1.0-1.5g/ml, cooling, filtering, filtrate is upper prop sample liquid, and upper prop sample liquid is passed through by 0.8BV applied sample amount and is equipped with
In the chromatographic column of resin, 95% ethyl alcohol of the distilled water of 12BV, 20% ethyl alcohol of 12BV, 70% ethyl alcohol of 10BV, 8BV are successively used
Gradient elution, flow velocity 2.5BV/h, 70% ethanol eluate are concentrated under reduced pressure, and it is that lily is total that drying, which simultaneously crushes to obtain pale yellow powder,
Saponin(e finished product；
(B) anemarrhena asphodefoides extract:
By the drying 12 hours of 100 DEG C of rhizoma ane marrhenae, until water content 10% is hereinafter, crushing, crosses 14 meshes, 3 times of 30% second of amount
Alcohol infiltrate 6h after, then use 30% ethanol percolation, diacolation speed be 1.2BV/h, collect 24 times of amount percolates, be concentrated under reduced pressure into density
For 1.0-1.5g/ml, cooling, filtering, filtrate is upper prop sample liquid, and upper prop sample liquid is passed through by 0.8BV applied sample amount and is equipped with
In the chromatographic column of resin, 95% ethyl alcohol of the distilled water of 12BV, 20% ethyl alcohol of 12BV, 70% ethyl alcohol of 10BV, 8BV are successively used
Gradient elution, flow velocity 2.5BV/h, 70% ethanol eluate are concentrated under reduced pressure, and it is that rhizoma anemarrhenae is total that drying, which simultaneously crushes to obtain pale yellow powder,
Saponin(e finished product.
(C) by anemarrhena asphodefoides extract made from Bulbus Lilii extract made from step (A) and step (B) according to anemarrhena total saponin:
The weight ratio of lilium total saponin be 1:2 combine to get.
It is as a preferred embodiment of the invention, Bulbus Lilii extract made from step (A) and step (B) is obtained
Anemarrhena asphodefoides extract is according to anemarrhena total saponin: the weight ratio of lilium total saponin is combined for 1:2.
To realize above-mentioned 4th purpose, the technical solution adopted by the present invention is that:
A kind of drug for treating cognitive disorder disease, the drug are by as above any traditional Chinese medicine extract composition
With made from pharmaceutic adjuvant.
As a preferred embodiment of the invention, the dosage form of the drug be tablet, pill, granule,
Injection.
To realize above-mentioned 5th purpose, the technical solution adopted by the present invention is that:
The preparation method of any drug as above, includes the following steps:
(A) Bulbus Lilii extract is prepared:
By the drying 12 hours of 100 DEG C of lily medicinal material, until 10% or less water content.It crushes, crosses 14 meshes, 3 times of 30% second of amount
Alcohol infiltrate 6h after, then use 30% ethanol percolation, diacolation speed be 1.2BV/h, collect 24 times of amount percolates, be concentrated under reduced pressure into density
For 1.0-1.5g/ml, cooling, filtering, filtrate is upper prop sample liquid, and upper prop sample liquid is passed through by 0.8BV applied sample amount and is equipped with
In the chromatographic column of resin, 95% ethyl alcohol of the distilled water of 12BV, 20% ethyl alcohol of 12BV, 70% ethyl alcohol of 10BV, 8BV are successively used
Gradient elution, flow velocity 2.5BV/h, 70% ethanol eluate are concentrated under reduced pressure, and it is that lily is total that drying, which simultaneously crushes to obtain pale yellow powder,
Saponin(e finished product；
(B) anemarrhena asphodefoides extract is prepared:
By the drying 12 hours of 100 DEG C of rhizoma ane marrhenae, until water content 10% is hereinafter, crushing, crosses 14 meshes, 3 times of 30% second of amount
Alcohol infiltrate 6h after, then use 30% ethanol percolation, diacolation speed be 1.2BV/h, collect 24 times of amount percolates, be concentrated under reduced pressure into density
For 1.0-1.5g/ml, cooling, filtering, filtrate is upper prop sample liquid.Upper prop sample liquid is passed through by 0.8BV applied sample amount and is equipped with
In the chromatographic column of resin, 95% ethyl alcohol of the distilled water of 12BV, 20% ethyl alcohol of 12BV, 70% ethyl alcohol of 10BV, 8BV are successively used
Gradient elution, flow velocity 2.5BV/h, 70% ethanol eluate are concentrated under reduced pressure, and it is that rhizoma anemarrhenae is total that drying, which simultaneously crushes to obtain pale yellow powder,
Saponin(e finished product.
(C) by anemarrhena asphodefoides extract made from Bulbus Lilii extract made from step (A) and step (B) according to anemarrhena total saponin:
The weight ratio of lilium total saponin be 1:2 combination, be added excipient substance to get.
Lily processed product in the present invention, commercially available, such as sweet lily.
Rhizoma anemarrhenae processed product in the present invention, it is commercially available.
Traditional Chinese medicine extract composition provided by the invention can use separately as drug, can also be used as raw material and be made
The dosage forms such as tablet, pill, granule, injection use, and when various dosage forms are made, various auxiliary materials are added according to the pharmacy prior art
.
The invention has the advantages that:
1, the complex chemical composition that the rhizoma anemarrhenae in rhizoma anemarrhenae Lilii Decoction, lily contain, including as saponin(e, double benzene pyrrones,
The Multiple components such as Anthraquinones, alkaloids, it is difficult to extract.The present invention extracts single medicinal material by optimizing extracting method
Validation studies, and filters out the optimal proportion of its active chemical, in conjunction with pharmacological evaluation, has been prepared a kind of for controlling
Treat the traditional Chinese medicine extract composition of cognitive disorder disease.
2, the present invention is proved by pharmacological evaluation, the traditional Chinese medicine extract composition tool that preparation method through the invention obtains
There is good anti-cognitive disorder.Anemarrhena asphodefoides extract containing anemarrhena total saponin and the lily containing lilium total saponin are extracted
Object composition traditional Chinese medicine extract composition collaboration proves effective, have curative effect it is good, convenient to take, it is stable and controllable for quality, can long-term preservation,
The low advantage of dosage, has a good application prospect.
Specific embodiment
The invention will be further elucidated with reference to specific embodiments.It should be understood that these embodiments are merely to illustrate this hair
It is bright rather than limit the scope of the invention.In addition, it should also be understood that, after having read the content of the invention recorded, art technology
Personnel can make various changes or modifications the present invention, and such equivalent forms equally fall within the application the appended claims and limited
Fixed range.
1 Bulbus Lilii extract of embodiment
By the drying 12 hours of 100 DEG C of lily medicinal material, until water content 9%, crushes, 14 meshes, 3 times of 30% ethyl alcohol of amount infiltrations are crossed
After 6h, then with 30% ethanol percolation, diacolation speed is 1.2BV/h, collects 24 times of amount percolates, is concentrated under reduced pressure into density as 1.0-
1.5g/ml, cooling, filtering, filtrate is upper prop sample liquid.Upper prop sample liquid is passed through by 0.8BV applied sample amount equipped with resin
In chromatographic column, successively washed with 95% ethanol gradient of the distilled water of 12BV, 20% ethyl alcohol of 12BV, 70% ethyl alcohol of 10BV, 8BV
It is de-, flow velocity 2.5BV/h.70% ethanol eluate is concentrated under reduced pressure, and drying simultaneously crushes to obtain pale yellow powder, obtains lilium total saponin and contains
Amount is 71.1%.
2 anemarrhena asphodefoides extract of embodiment
By the drying 12 hours of 100 DEG C of rhizoma ane marrhenae, until water content 10% is hereinafter, crushing, crosses 14 meshes.3 times of 30% second of amount
Alcohol infiltrate 6h after, then use 30% ethanol percolation, diacolation speed be 1.2BV/h, collect 24 times of amount percolates, be concentrated under reduced pressure into density
For 1.0-1.5g/ml, cooling, filtering, filtrate is upper prop sample liquid.Upper prop sample liquid is passed through by 0.8BV applied sample amount and is equipped with
In the chromatographic column of resin, 95% ethyl alcohol of the distilled water of 12BV, 20% ethyl alcohol of 12BV, 70% ethyl alcohol of 10BV, 8BV are successively used
Gradient elution, flow velocity 2.5BV/h.70% ethanol eluate is concentrated under reduced pressure, and drying simultaneously crushes to obtain pale yellow powder, and it is total to obtain rhizoma anemarrhenae
Saponin content is 82.3%.
3 lily anemarrhena asphodefoides extract composition of embodiment
By anemarrhena total saponin and lilium total saponin obtained in embodiment 1 and embodiment 2 according to weight are as follows: rhizoma anemarrhenae
Total saposins: lilium total saponin=1:2 combination obtains rhizoma anemarrhenae Bulbus Lilii extract composition.
Influence test of 4 traditional Chinese medicine extract composition of embodiment to depression model rat
1. experimental material
1.1 animal
SD male rat 70 (Second Military Medical University, PLA's animal center), weight 180-220g.
1.2 experimental drug
Prozac, traditional rhizoma anemarrhenae lily decocting liquid (take lily 150g, first impregnate 12h with clear water 300mL, remove water, then plus steam
Distilled water 500mL intense fire is changed to mild fire (about 20min) after being fried open (about 18min), obtains lily decoction liquor 400mL；Rhizoma anemarrhenae 75g is taken, is added
Distilled water 150mL, the same method obtain rhizoma anemarrhenae decoction liquor 200mL.Gained lily, rhizoma anemarrhenae decoction liquor are mixed, mild fire is concentrated (about
45min) to 1g/mL), 1 Bulbus Lilii extract of embodiment, 2 anemarrhena asphodefoides extract of embodiment, the combination of 3 lily anemarrhena asphodefoides extract of embodiment
Object.
2 experimental methods
2.1 animal model
All rat single cage raisings, continuously receive a variety of different stress stimulations of 35d, comprising: 4 DEG C of ice water swimming 5min,
Turn on light (incandescent lamp, 5W, 900cm overnight2), folder tail 10min, inclination 45 spend night, prohibit water for 24 hours, foot shock (voltage
50mV, every 30s stimulate 1 time, continue 10s, totally 8 times), fasting for 24 hours, (daytime sprinkles 300mL water in padding to wet cage, after 8h
Change dry padding).Receive 2 kinds of different stimulateds at random daily, but given stimulation is all different in continuous 2d, is carried out after last stimulation each
Item Indexs measure.
2.2 administration groupings
Depression rat is randomly divided into 7 groups, every group 10, it may be assumed that
Blank control group
Model group
Positive drug group (Prozac 10mg/kg/d)
Traditional rhizoma anemarrhenae lily decocting liquid group (dosage 5g/kg)
1 Bulbus Lilii extract group (dosage 95mg/kg) of embodiment
2 anemarrhena asphodefoides extract group (dosage 95mg/kg) of embodiment
3 rhizoma anemarrhenae Bulbus Lilii extract composition group (1:2, dosage 95mg/kg) of embodiment
It is administered in modeling, gavages administration within continuous 4 weeks.
2.3 observation index
2.3.1 open field test
Rat is put among spacious case, records the quantity that rat in 3min passes through grid, more than tripodia passing through a lattice is 1
Point, horizontal through grid sum be horizontal movement score；The vertical liftoff number of its biped is counted simultaneously, it is vertical liftoff primary
It is 1 point, vertical liftoff number is the score of rat vertical movement.Horizontal movement and vertical movement summation are that spacious field moves
Point.This test can detect rat autonomic activities ability and to extraneous curious degree, chronic stress treated rat spacious field examination
Yan get branch is substantially reduced.As a result it see the table below 1.
Table 1, each group rat spacious field experimental result (x ± s, n=10 divide)
Note: compared with blank control group,#P<0.05,##P<0.01；Compared with model group,*P < 0.05,**P<0.01
After modeling, the level of model group rats passes through lattice number, erects number substantially less than blank control group (P < 0.01),
Illustrate that depressive symptom, modeling success occurs in rat；Compared with model group, traditional rhizoma anemarrhenae lily decocting liquid group, 1 lily of example are extracted
The level of object group, 2 anemarrhena asphodefoides extract group of example and Prozac group rat passes through lattice number, setting number has increase, and difference has
Statistical significance (P < 0.05, P < 0.01)；The level of 3 rhizoma anemarrhenae Bulbus Lilii extract composition group rat of example is passed through lattice number, is erect
Number has increase, and difference has statistical significance (P < 0.05, P < 0.01).
2.3.2 forced swimming test
Rat is put into diameter 20cm, in the transparent water vat of deep 70cm, is kept for 25 DEG C of water temperature, is put into the swimming of rat adaptability
1min, then record in rat 4min swimming motionless time, this test can reflect rat, and in case of emergency instinct is sought survival
Desire, the non-swimming time of rat can extend after chronic stress processing.As a result 2 be see the table below.
Table 2, the comparison of each group rats'swimming dead time (x ± s, n=16)
Note: compared with blank control group,#P<0.05,##P<0.01；Compared with model group,*P < 0.05,**P<0.01
After modeling, compared with blank control group, the model group rats forced swimming dead time significantly extends (P < 0.01),
There is depressive symptom in rat；Compared with model group, traditional rhizoma anemarrhenae lily decocting liquid group, 1 Bulbus Lilii extract group of example, example 2 are known
Female extract group and Prozac group rat dead time are reduced, and difference has statistical significance (P < 0.05), 3 rhizoma anemarrhenae hundred of example
It closes the reduction of extractive composition group rat dead time to be especially apparent, difference has statistical significance (P < 0.01).
2.3.3 5-HT, CORT and rush ACTH level detection
3% yellow Jackets 0.3-0.4mL injects intraperitoneal anesthesia rat, and abdominal aorta takes 5mL blood, retains mark
This.Centrifuge is put into after room temperature standing 1h to be centrifuged.4 DEG C, 1500r/min, it is centrifuged 15min, takes top serum, -80 DEG C of ice
Case saves.5-HT, CORT in serum are examined with ELISA method according to kit specification and promote ACTH level.As a result 3 be see the table below.
The comparison (x ± s, n=8) of 5-HT, CORT, ACTH amount in table 3, each group rat blood serum
Note: compared with blank control group,#P<0.05,##P<0.01；Compared with model group,*P < 0.05,**P<0.01
5-HT, CORT, ACTH level are substantially less than blank control group (P < 0.01) in model group rats serum.With model
Group is compared, the horizontal significant raising (P < 0.01) of 5-HT, CORT, ACTH of Prozac group；Traditional rhizoma anemarrhenae lily decocting liquid group 5-
The horizontal significant raising (P < 0.01) of HT, CORT, ACTH；Horizontal significant raising (the P < of 1 Bulbus Lilii extract group CORT, ACTH of example
0.01)；The horizontal significant raising (P < 0.01) of 2 anemarrhena asphodefoides extract group CORT, ACTH of example；The combination of 3 rhizoma anemarrhenae Bulbus Lilii extract of example
5-HT, CORT, ACTH level raising of object group rat are especially apparent, and difference has statistical significance (P < 0.01).
3 conclusions
Rhizoma anemarrhenae Lilii Decoction active principle (traditional Chinese medicine extract composition) prepared by the present invention can increase 5-HT in serum,
The level of CORT, ACTH improve Behavioral feature, improve and alleviate the symptom of depression, and therapeutic effect compares traditional water decoction
Liquid is more significant, has the function for the treatment of cognitive disorder disease, and compared with traditional rhizoma anemarrhenae lily decocting liquid, Chinese medicine of the invention
Extractive composition dosage is low, convenient to take, quality controllable, in addition present invention optimization Bulbus Lilii extract and anemarrhena asphodefoides extract
Preparation method, obtained Bulbus Lilii extract and anemarrhena asphodefoides extract content are high.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
Member, without departing from the principle of the present invention, can also make several improvement and supplement, these are improved and supplement also should be regarded as
Protection scope of the present invention.
Claims (8)
1. a kind of traditional Chinese medicine extract composition for treating cognitive disorder disease, which is characterized in that the Chinese medical extract combination
Object is made of the anemarrhena asphodefoides extract containing anemarrhena total saponin and the Bulbus Lilii extract containing lilium total saponin, the Chinese medical extract
Anemarrhena total saponin in composition: the weight ratio of lilium total saponin is 1:2；
The anemarrhena asphodefoides extract and Bulbus Lilii extract the preparation method is as follows:
(A) Bulbus Lilii extract:
By the drying 12 hours of 100 DEG C of lily medicinal material, until water content is 0.01%-10%, crushes, crosses 14 meshes, 3 times of 30% second of amount
Alcohol infiltrates 6h, is then that 1.2BV/h carries out diacolation with diacolation speed with 30% ethyl alcohol, collects 24 times of amount percolates, is concentrated under reduced pressure
It is 1.0-1.5g/ml to density, cooling, filtering, filtrate is upper prop sample liquid, and upper prop sample liquid is led to by 0.8BV applied sample amount
Enter in the chromatographic column equipped with resin, successively with the distilled water of 12BV, 20% ethyl alcohol of 12BV, 70% ethyl alcohol of 10BV, 8BV
95% ethyl alcohol is that 2.5BV/h carries out gradient elution with flow velocity, and 70% ethanol eluate is concentrated under reduced pressure, and drying simultaneously crushes faint yellow
Powder is lilium total saponin finished product；
(B) anemarrhena asphodefoides extract:
By the drying 12 hours of 100 DEG C of rhizoma ane marrhenae, until water content 0.01%-10%, crushes, crosses 14 meshes, 3 times of 30% ethyl alcohol of amount
6h is infiltrated, is then that 1.2BV/h carries out diacolation with diacolation speed with 30% ethyl alcohol, collects 24 times of amount percolates, be concentrated under reduced pressure into
Density is 1.0-1.5g/ml, and cooling, filtering, filtrate is upper prop sample liquid, and upper prop sample liquid is passed through by 0.8BV applied sample amount
In chromatographic column equipped with resin, successively with the distilled water of 12BV, 20% ethyl alcohol of 12BV, 70% ethyl alcohol of 10BV, 8BV 95%
Ethyl alcohol is that 2.5BV/h carries out gradient elution with flow velocity, and 70% ethanol eluate is concentrated under reduced pressure, and drying simultaneously crushes to obtain yellowish toner
End is anemarrhena total saponin finished product.
2. traditional Chinese medicine extract composition according to claim 1, which is characterized in that Bulbus Lilii extract made from step (A)
The average content of middle lilium total saponin is 70%-100%；The content of anemarrhena total saponin in anemarrhena asphodefoides extract made from step (B)
For 70%-100%.
3. the traditional Chinese medicine extract composition as described in claim 1-2 is any is in the drug of preparation treatment cognitive disorder disease
Using.
4. application according to claim 3, which is characterized in that the cognitive disorder disease refer to phychasthnia, hysteria,
Depression, senile dementia, schizophrenia.
5. a kind of preparation method for the traditional Chinese medicine extract composition for treating cognitive disorder disease, which is characterized in that including walking as follows
It is rapid:
(A) Bulbus Lilii extract:
By the drying 12 hours of 100 DEG C of lily medicinal material, until water content is 0.01%-10%, crushes, crosses 14 meshes, 3 times of 30% second of amount
Alcohol infiltrates 6h, is then that 1.2BV/h carries out diacolation with diacolation speed with 30% ethyl alcohol, collects 24 times of amount percolates, is concentrated under reduced pressure
It is 1.0-1.5g/ml to density, cooling, filtering, filtrate is upper prop sample liquid, and upper prop sample liquid is led to by 0.8BV applied sample amount
Enter in the chromatographic column equipped with resin, successively with the distilled water of 12BV, 20% ethyl alcohol of 12BV, 70% ethyl alcohol of 10BV, 8BV
95% ethyl alcohol is that 2.5BV/h carries out gradient elution with flow velocity, and 70% ethanol eluate is concentrated under reduced pressure, and drying simultaneously crushes faint yellow
Powder is lilium total saponin finished product；
(B) anemarrhena asphodefoides extract:
By the drying 12 hours of 100 DEG C of rhizoma ane marrhenae, until water content 0.01%-10%, crushes, crosses 14 meshes, 3 times of 30% ethyl alcohol of amount
6h is infiltrated, is then that 1.2BV/h carries out diacolation with diacolation speed with 30% ethyl alcohol, collects 24 times of amount percolates, be concentrated under reduced pressure into
Density is 1.0-1.5g/ml, and cooling, filtering, filtrate is upper prop sample liquid, and upper prop sample liquid is passed through by 0.8BV applied sample amount
In chromatographic column equipped with resin, successively with the distilled water of 12BV, 20% ethyl alcohol of 12BV, 70% ethyl alcohol of 10BV, 8BV 95%
Ethyl alcohol is that 2.5BV/h carries out gradient elution with flow velocity, and 70% ethanol eluate is concentrated under reduced pressure, and drying simultaneously crushes to obtain yellowish toner
End is anemarrhena total saponin finished product；
(C) by anemarrhena asphodefoides extract made from Bulbus Lilii extract made from step (A) and step (B) according to anemarrhena total saponin: lily
The weight ratio of total saposins be 1:2 combine to get.
6. a kind of drug for treating cognitive disorder disease, which is characterized in that the drug is by as described in claim 1-2 is any
Traditional Chinese medicine extract composition and pharmaceutic adjuvant made from.
7. drug according to claim 6, which is characterized in that the dosage form of the drug is tablet, pill, particle
Agent, injection.
8. the preparation method of drug as claimed in claim 6, which comprises the steps of:
(A) Bulbus Lilii extract:
By the drying 12 hours of 100 DEG C of lily medicinal material, until water content is 0.01%-10%, crushes, crosses 14 meshes, 3 times of 30% second of amount
Alcohol infiltrates 6h, is then that 1.2BV/h carries out diacolation with diacolation speed with 30% ethyl alcohol, collects 24 times of amount percolates, is concentrated under reduced pressure
It is 1.0-1.5g/ml to density, cooling, filtering, filtrate is upper prop sample liquid, and upper prop sample liquid is led to by 0.8BV applied sample amount
Enter in the chromatographic column equipped with resin, successively with the distilled water of 12BV, 20% ethyl alcohol of 12BV, 70% ethyl alcohol of 10BV, 8BV
95% ethyl alcohol is that 2.5BV/h carries out gradient elution with flow velocity, and 70% ethanol eluate is concentrated under reduced pressure, and drying simultaneously crushes faint yellow
Powder is lilium total saponin finished product；
(B) anemarrhena asphodefoides extract:
By the drying 12 hours of 100 DEG C of rhizoma ane marrhenae, until water content 0.01%-10%, crushes, crosses 14 meshes, 3 times of 30% ethyl alcohol of amount
6h is infiltrated, is then that 1.2BV/h carries out diacolation with diacolation speed with 30% ethyl alcohol, collects 24 times of amount percolates, be concentrated under reduced pressure into
Density is 1.0-1.5g/ml, and cooling, filtering, filtrate is upper prop sample liquid, and upper prop sample liquid is passed through by 0.8BV applied sample amount
In chromatographic column equipped with resin, successively with the distilled water of 12BV, 20% ethyl alcohol of 12BV, 70% ethyl alcohol of 10BV, 8BV 95%
Ethyl alcohol is that 2.5BV/h carries out gradient elution with flow velocity, and 70% ethanol eluate is concentrated under reduced pressure, and drying simultaneously crushes to obtain yellowish toner
End is anemarrhena total saponin finished product；
(C) by anemarrhena asphodefoides extract made from Bulbus Lilii extract made from step (A) and step (B) according to anemarrhena total saponin: lily
The weight ratio of total saposins be 1:1 combination, and be added excipient substance to get.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110585224A (en) * | 2019-10-11 | 2019-12-20 | 上海长海医院 | Application of timosaponin BI, timosaponin IA and timosaponin AIII in preparation of medicine for treating cognitive disorder |
| CN113785777A (en) * | 2021-08-20 | 2021-12-14 | 南京时光标生物科技有限公司 | Strongly hydrophilic and agglomerated cat litter and preparation process thereof |
-
2019
- 2019-10-11 CN CN201910963970.4A patent/CN110507753A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 李子雯等: "百合知母汤总皂苷调节5_HT功能治疗抑郁症的作用机制", 《中国实验方剂学杂志》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110585224A (en) * | 2019-10-11 | 2019-12-20 | 上海长海医院 | Application of timosaponin BI, timosaponin IA and timosaponin AIII in preparation of medicine for treating cognitive disorder |
| CN113785777A (en) * | 2021-08-20 | 2021-12-14 | 南京时光标生物科技有限公司 | Strongly hydrophilic and agglomerated cat litter and preparation process thereof |
| CN113785777B (en) * | 2021-08-20 | 2022-12-20 | 南京时光标生物科技有限公司 | Strongly hydrophilic and agglomerated cat litter and preparation process thereof |
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Application publication date: 20191129 |