CN110495275A - A kind of Soil oxidation Asia nitrogen discharge-reducing method - Google Patents

A kind of Soil oxidation Asia nitrogen discharge-reducing method Download PDF

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CN110495275A
CN110495275A CN201910790634.4A CN201910790634A CN110495275A CN 110495275 A CN110495275 A CN 110495275A CN 201910790634 A CN201910790634 A CN 201910790634A CN 110495275 A CN110495275 A CN 110495275A
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soil
leaching liquor
camphortree leaf
leaf
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CN110495275B (en
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张令
高宇
方海富
朱丛飞
罗汉东
王佰慧
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Jiangxi Agricultural University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • C09K17/00—Soil-conditioning materials or soil-stabilising materials
    • C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
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Abstract

The invention belongs to the technical field of reduction of greenhouse gas discharge, specially a kind of Soil oxidation Asia nitrogen discharge-reducing method, comprising the following steps: collect camphor tree Litter leaf, clean, air-dry；It shreds to obtain the camphor tree Litter leaf of fragment shape, deionized water is added, then extracting at constant temperature vibrates, filters, obtain camphortree leaf leaching liquor, leaching liquor is applied to soil, monitors the discharge of Soil oxidation Asia nitrogen.As a result, it has been found that after camphortree leaf leaching liquor processing soil, the sub- nitrogen discharged significant decrease of Soil oxidation.Camphor tree Litter leaf source is wide, at low cost, using flooding, simplifies integrated operation, is a kind of convenient for popularization, cost-effective nitrous oxide discharge-reducing method.

Description

A kind of Soil oxidation Asia nitrogen discharge-reducing method
Technical field
The invention belongs to the technical field of reduction of greenhouse gas discharge, specially a kind of Soil oxidation Asia nitrogen discharge-reducing method.
Background technique
Soil oxidation Asia nitrogen is important greenhouse gases, leads to greenhouse effects, also results in soil nitrogen discharge, influence Soil Nitrogen Validity.Nitrous oxide can participate in various photochemical reactions and influence ozone layer stability, endanger mankind's body dis environment and Social sustainable development.In nitrous oxide source, soil is most important emission source, about occupy the half of atmosphere total release with On.Therefore, to preserving the ecological environment, mitigation of climate change improves Soil Nitrogen and keeps and validity meaning the emission reduction of Soil oxidation Asia nitrogen Justice is great.
Currently, proposing a variety of methods for the nitrogen emission reduction of Soil oxidation Asia, including apply chemical nitrification inhibitor, biological nitre Change the measures such as inhibitor, but nitrification inhibitor production cost is higher, it is difficult to effectively popularize in an all-round way.Chinese patent CN102308687A discloses a kind of straw biological black wood charcoal agent, soil treatment method for reducing farmland nitrous oxide emission, wherein with straw Stalk is raw material, and thermal cracking, grinding obtain the small biological black wood charcoal of partial size, then is spread fertilizer over the fields in soil surface, is mixed by ploughing rake with soil Uniformly, realize that attenuating Soil oxidation Asia is nitrogen discharged.The preparation of biological black wood charcoal needs to expend a large amount of energy, and production operation is complicated, It is not energy saving enough.A kind of Soil oxidation Asia nitrogen discharge-reducing method with promotion potential is found to have important practical significance.
Camphor tree (Cinnamomum camphora (L.) Presl) also known as cinnamomum camphora, cultivation is universal, and blade source is wide. Research for camphortree leaf is more, but the focus studied is gathered in the extraction lignanoid, flavonoid from camphortree leaf mostly On the compounds such as object and d-Bomeol, or using camphortree leaf prepare insecticide, pesticide and compound fertilizer etc..Camphortree leaf is in greenhouse There is not been reported for the research of gas discharge aspect, and camphor tree Litter leaf waste treatment, utilization is insufficient, leads to environmental pollution and resource Waste.
Summary of the invention
This application provides a kind of economically viable Soil oxidation Asia nitrogen discharge-reducing methods, by being original with camphor tree Litter leaf Material, adds flooding to obtain leaching liquor, then be applied to soil surface, under the nitrous oxide emission rate in monitoring discovery soil is significant Drop.The Soil oxidation Asia nitrogen discharge-reducing method is simple, at low cost, convenient for promoting the use of.
Specifically, in view of the deficiencies of the prior art, the present invention provides the following technical scheme that
On the one hand, this application provides a kind of Soil oxidation Asia nitrogen discharge-reducing methods, comprising the following steps:
Camphortree leaf is collected, is cleaned, is air-dried；
Air-dried camphortree leaf is taken, shreds to obtain the camphortree leaf of fragment shape, deionized water, extracting at constant temperature, then oscillation, mistake is added Filter, obtains camphortree leaf leaching liquor,
Camphortree leaf leaching liquor is applied to soil, monitors the discharge of Soil oxidation Asia nitrogen.
Preferably, the camphortree leaf is camphor tree Litter leaf, and the camphor tree Litter leaf after air-drying is shredded into length and width and is existed The strip fragment of 2-3mm.
Preferably, the solid-to-liquid ratio of the deionized water of the camphortree leaf and addition of fragment shape is 1g:19mL.
Preferably, the temperature of extracting at constant temperature is 35-50 DEG C, extraction time 1-2h.
Preferably, the temperature of extracting at constant temperature is 50 DEG C, extraction time 1h, and the frequency of oscillation is 80Hz, vibrates 30min.
On the other hand, this application provides a kind of camphortree leaf leaching liquor, preparation process includes:
Collect camphor tree Litter leaf, clean, air-dry, shred to obtain length and width in the strip fragment of 2-3mm, addition go from Sub- water, 35-50 DEG C of extracting at constant temperature 1-2h, oscillation, filtering obtain camphortree leaf leaching liquor.
Preferably, the solid-to-liquid ratio of the deionized water of the camphortree leaf and addition of strip fragment is 1g:19mL in preparation process.
Preferably, above-mentioned camphortree leaf leaching liquor is for reducing the greenhouse gas emission in soil.
Preferably, above-mentioned camphortree leaf leaching liquor discharges for reducing the nitrous oxide gas in soil.
The prior art is compared, and the effect of the application and is had an advantage that:
1. especially using camphor tree Litter leaf as raw material, realizing refuse reclamation using camphortree leaf as raw material, Litter leaf being avoided to burn Environmental pollution caused by burning；Raw material is easy to get, at low cost.
2. the leaching liquor that camphortree leaf adds flooding to obtain can be effectively reduced the discharge of Soil oxidation Asia nitrogen, increase soil Nitrogen fixing capacity.With flooding, avoid using organic solvent, it is easy to operate, while reducing the pollution to environment.Meanwhile it keeping away Exempt from camphortree leaf and directly applies the potential impact reduced to nitrogen availa-bility in soil and the pest and disease damage that may cause.
3. mechanical shaking extraction again after extracting at constant temperature, improves production efficiency at the time needed for shortening extraction；The application simultaneously The preparation process of the camphortree leaf leaching liquor of offer is simple, easily operated, economical and practical, convenient for promoting on a large scale for reducing soil Earth nitrous oxide emission.
4. the application is studies have shown that camphortree leaf leaching liquor and wind desiceted soil monitor soil according to the ratio mixing of 1ml:3.5g Gas discharge, discovery start the 1st day calculated after preculture 2 days, the rate of discharge of the Soil oxidation Asia nitrogen of leaching liquor processing Significantly lower than blank control group and camphortree leaf group.
Camphortree leaf withers and falls to soil, need to start within the 3rd day after preculture, and nitrous oxide emission rate reduces, but camphor tree Tree Litter leaf will increase the carbon input of soil during being decomposed by the microorganisms, influence nitrogen absorption, lead to pest and disease damage.In addition, camphor tree The Soil oxidation Asia nitrogen accumulation rate of discharge of tree Litter leaf group is apparently higher than camphortree leaf leaching liquor processing group, therefore camphortree leaf leaching liquor It is to reduce the sub- nitrogen discharged cost-effective mode of Soil oxidation.
Detailed description of the invention
Fig. 1 is the Soil oxidation Asia nitrogen accumulation rate of discharge figure of measurement；
Wherein, leaching liquor prepared by curve 1- embodiment 1, leaching liquor prepared by 2- embodiment 2, prepared by 3- embodiment 3 Leaching liquor, leaching liquor prepared by 4- embodiment 4, leaching liquor prepared by 5- embodiment 5, leaching liquor prepared by 6- embodiment 6,7- are real Apply the leaching liquor of the preparation of example 7, leaching liquor prepared by 8- embodiment 8,9- camphor tree Litter leaf group, 10- blank control group.
Specific embodiment
This application provides a kind of Soil oxidation Asia nitrogen discharge-reducing method, process includes preparing camphortree leaf leaching liquor, will be soaked Extract is applied to soil, monitors nitrous oxide emission.
The preparation process of camphortree leaf leaching liquor includes: to collect camphor tree Litter leaf, is cleaned, and air-dries, shreds to obtain length and width The strip fragment in 2-3mm is spent, deionized water (solid-to-liquid ratio of camphortree leaf fragment and deionized water is 1g:19mL), 35-50 is added DEG C extracting at constant temperature 1-2h, 50-90Hz mechanical shaking extraction 30-60min, filtering, obtains camphortree leaf leaching liquor.
It is found in test, the frequency of oscillation of mechanical shaking extraction is little to the components influence of leaching liquor, preferably 80Hz.
In the course of the research, use is camphor tree Litter leaf to the technical solution of the application.
The component in the camphortree leaf leaching liquor prepared in following each embodiments is analyzed, analysis method is as follows:
One, instrument: Agilent 6560qtof
Two, test condition:
Liquid phase chromatogram condition chromatographic column: waters ACCQ-TAG ULTRA C18,2.1 × 100mm, 1.7 μm, part number 186003837:
Flow velocity: 0.3mL/min；
Column temperature: 35 DEG C；
Sampling volume: 1 μ L；
DAD detection: 210nm, 254nm, 260nm, 280nm；
Mobile phase: A) contain 0.1% aqueous formic acid B) methanol；
Gradient program: 0-2min, 7%B；2-20min, 93%B；20-26,95%B；26-27min, 7%B, operation 5min。
Mass Spectrometry Conditions: 6560 HPLC-Q-TOF MS/MS of Agilent
Ion source: the dry temperature degree of ESI: 320 DEG C；Dry gas stream speed: 10L/min；
Nebulizer pressure: 35psi sheath temperature degree: 350 DEG C, sheath gas: 12L/min
Fragmentation voltage: 135v
Scanning mode: level-one is swept entirely, second level MS/MS mode, collision energy 10,30eV.Level-one and second level scanning quality number Range m/z 100-1700
Three, test sample prepares
Leaching liquor sample 1mL is taken, 1mL hplc grade methanol is added, shakes 3min, uses 0.22um saliva dragon membrane filtration, dress Enter sample injection bottle.
Four, database retrieval
Based on the compound in Agilent MSC software and distinctive TCM TCM Databases identification sample.
It is further described the application below by specific embodiment, it should be noted that, following embodiment is only pair The application's enumerates, and technical solution described herein and protection scope are not limited to each embodiment.
Embodiment 1
Collect camphor tree Litter leaf, clean, air-dry, shred to obtain length and width in the strip fragment of 2-3mm, addition go from Sub- water, dosage are placed in 35 DEG C of waters bath with thermostatic control according to the calculating of 19mL deionized water is added in every 1g camphortree leaf fragment and extract 1h, so Afterwards in 80Hz mechanical shaking extraction 30min, filtering obtains camphortree leaf leaching liquor.
Using the component in preceding method analysis leaching liquor, 1 the results are shown in Table:
Table 1.
Compound name Molecular formula Chinese
8-O-Acetyl shanzhiside methyl ester C19H28O12 Cyclosporin glucoside
Acetylcatalpol C18H26O10 Acetylcatalpol
Afzelin C21H20O10 Afzelin
Aromadendrin-5,7-dimethyl ether C15H12O5 Armomadendrin -5,7- dimethyl ether
Chlorogenic acid C16H18O9 Chlorogenic acid
Cimidahurine C14H20O8 *
Dehydromorroniaglycone C11H14O5 It is dehydrated Monot's aglycon
Floripavine C19H21NO4 Sinoacutine
Gerberinside C16H18O8 *
Hyperin C21H20O12 *
Isorhamnetin C16H12O7 Isorhamnetin
Laurolitsine C18H19NO4 *
Picrodendrin A C21H28O10 *
Quercetin-3-O-alpha-L-rhamnopyranoside C21H20O11 *
Embodiment 2
Collect camphor tree Litter leaf, clean, air-dry, shred to obtain length and width in the strip fragment of 2-3mm, addition go from Sub- water, dosage are placed in 35 DEG C of waters bath with thermostatic control according to the calculating of 19mL deionized water is added in every 1g camphortree leaf fragment and extract 1h, so Afterwards in 80Hz mechanical shaking extraction 60min, filtering obtains camphortree leaf leaching liquor.
Using the component in preceding method analysis leaching liquor, it the results are shown in Table 2:
Table 2.
Embodiment 3
Collect camphor tree Litter leaf, clean, air-dry, shred to obtain length and width in the strip fragment of 2-3mm, addition go from Sub- water, dosage are placed in 35 DEG C of waters bath with thermostatic control according to the calculating of 19mL deionized water is added in every 1g camphortree leaf fragment and extract 2h, so Afterwards in 80Hz mechanical shaking extraction 30min, filtering obtains camphortree leaf leaching liquor.
Using the component in preceding method analysis leaching liquor, it the results are shown in Table 3:
Table 3.
Embodiment 4
Collect camphor tree Litter leaf, clean, air-dry, shred to obtain length and width in the strip fragment of 2-3mm, addition go from Sub- water, dosage are placed in 35 DEG C of waters bath with thermostatic control according to the calculating of 19mL deionized water is added in every 1g camphortree leaf fragment and extract 2h, so Afterwards in 80Hz mechanical shaking extraction 60min, filtering obtains camphortree leaf leaching liquor.
Using the component in preceding method analysis leaching liquor, it the results are shown in Table 4:
Table 4.
Embodiment 5
Collect camphor tree Litter leaf, clean, air-dry, shred to obtain length and width in the strip fragment of 2-3mm, addition go from Sub- water, dosage are placed in 50 DEG C of waters bath with thermostatic control according to the calculating of 19mL deionized water is added in every 1g camphortree leaf fragment and extract 1h, so Afterwards in 80Hz mechanical shaking extraction 30min, filtering obtains camphortree leaf leaching liquor.
Using the component in preceding method analysis leaching liquor, it the results are shown in Table 5:
Table 5.
Embodiment 6
Collect camphor tree Litter leaf, clean, air-dry, shred to obtain length and width in the strip fragment of 2-3mm, addition go from Sub- water, dosage are placed in 50 DEG C of waters bath with thermostatic control according to the calculating of 19mL deionized water is added in every 1g camphortree leaf fragment and extract 1h, so Afterwards in 80Hz mechanical shaking extraction 60min, filtering obtains camphortree leaf leaching liquor.
Using the component in preceding method analysis leaching liquor, it the results are shown in Table 6:
Table 6.
Embodiment 7
Collect camphor tree Litter leaf, clean, air-dry, shred to obtain length and width in the strip fragment of 2-3mm, addition go from Sub- water, dosage are placed in 50 DEG C of waters bath with thermostatic control according to the calculating of 19mL deionized water is added in every 1g camphortree leaf fragment and extract 2h, so Afterwards in 80Hz mechanical shaking extraction 30min, filtering obtains camphortree leaf leaching liquor.
Using the component in preceding method analysis leaching liquor, it the results are shown in Table 7:
Table 7.
Embodiment 8
Collect camphor tree Litter leaf, clean, air-dry, shred to obtain length and width in the strip fragment of 2-3mm, addition go from Sub- water, dosage are placed in 50 DEG C of waters bath with thermostatic control according to the calculating of 19mL deionized water is added in every 1g camphortree leaf fragment and extract 2h, so Afterwards in 80Hz mechanical shaking extraction 60min, filtering obtains camphortree leaf leaching liquor.
Using the component in preceding method analysis leaching liquor, it the results are shown in Table 8:
Table 8.
According to the content in table 1-8 it is found that containing component in the camphortree leaf leaching liquor of the application preparation: cyclosporin Portugal Polyglycoside (8-O-Acetyl shanzhiside methyl ester), acetylcatalpol (Acetylcatalpol), afzelin (Afzelin) and chlorogenic acid (Chlorogenic acid)；There are also 7 groups of leaching liquors contain component Cimidahurine and Picrodendrin A, 6 groups of leaching liquors contain Gerberinside and Quercetin-3-O-alpha-L- rhamnopyranoside。
Embodiment 9
After the camphortree leaf leaching liquor application soil for studying embodiment 1-8 preparation, the discharge of nitrous oxide in soil.
Specific research method is as follows:
Blank control group (CK), camphor tree Litter leaf group, camphortree leaf leaching liquor group (1. -8.) are set, and every group setting 3 parallel Test.
Weighing 35g wind desiceted soil respectively, (soil sample uses the fresh soil of hayashishita, and the acquisition time of each group soil sample is identical with position, every 15g The aqueous 0.29g of wind desiceted soil) it is placed in 150mL conical flask, 0.5g camphor tree Litter leaf is added in 3 conical flasks, adds in 3 conical flasks and goes The aforementioned preparation of 10ml is added dropwise with liquid-transfering gun as blank control group, camphortree leaf leaching liquor group (24 conical flasks) in ionized water Camphortree leaf leaching liquor.
It is 60%WHC (maxmun field capacity of soil used), camphor tree Litter leaf group for water content in control wind desiceted soil The deionized water volume being added with blank control group is 12.36mL, and leaching liquor group has additionally incorporated 2.36mL deionized water.
Taper bottle cap is sealed with preservative film in incubation, and pricks 6 apertures, the container of measurement in every two days in top Gross mass supplements deionized water to keep soil moisture.After 25 ± 1 DEG C of constant temperature are protected from light preculture 2d, start timing and the 1,3,6,10,15,21,28,35,42 day progress gas collecting.Gas collection method are as follows:
Firstly, each conical flask preservative film is removed, is about divulged information 10 seconds with air blower to flask, rubber stopper is installed on On conical flask, the fresh air of 40ml is squeezed into conical flask with syringe, continuous to take out 10 times, gas mixing is uniform, acquisition Gas 40ml, the triple valve for turning off rubber stopper are sealed, and are then placed in incubator and are continued to cultivate 2 hours, carry out gas again Body acquisition.Using the gas chromatograph (Agilent for being equipped with flame ionization detector (FID) and electron capture detector (ECD) 7890B, Santa Clara, CA, USA) determine the N of each gaseous sample2O concentration calculates rate of discharge and accumulation discharge speed Rate.
Rate of discharge is calculated by following equation:
In above formula: E refers to soil N2O(ng.g-1.h-1) rate of discharge, P is standard atmospheric pressure (Pa), and V is at the top of conical flask Space (cm3), c is N2O (ppb) concentration, △ c indicate the N measured after gas collecting twice2O (ppb) concentration difference；
△ t is gas collection interval time (h), and R is universal gas constant (8.314Jmol-1·K-1), T is absolute gas Warm (K), M is N2O(g·mol-1) molecular mass, m is the oven-dried weight (g) of culture soil.
Accumulate the calculation method of rate of discharge
M=Σ (Fi+1+Fi)/2*(ti+1- ti)*24
In formula: M is soil N2O accumulates rate of discharge, μ gm-2；F is N2O emission flux, μ gm2·h-1；I is sampling Number；ti+1- tiSampling interval number of days.
Measurement the results are shown in Table 9 and table 10.
The sub- nitrogen discharged rate (ngg of 9. Soil oxidation of table-1·h-1)
Number of days 1 3 6 10 15
Extraction group is 1. 0.291447 0.669076 0.319025 0.448311 0.125132
Extraction group is 2. 0.490201 0.218928 0.460348 0.304318 0.074257
Extraction group is 3. 0.011839 0.259179 0.114324 0.41025 0.173535
Extraction group is 4. -0.04007 0.308104 0.213729 0.37517 0.149093
Extraction group is 5. -0.30704 0.209618 0.486768 0.244655 0.220101
Extraction group is 6. -0.34482 0.473671 0.206933 0.40515 0.201607
Extraction group is 7. 0.408823 0.30583 0.258049 0.196959 0.24279
Extraction group is 8. 0.231954 0.376329 0.147567 0.339737 0.102442
Camphor tree fallen leaves group 2.331466 0.164705 0.211045 0.229552 0.135163
CK control group 2.603148 0.859084 0.690381 0.392548 0.358584
Number of days 21 28 35 42
Extraction group is 1. -0.0574 0.16551 0.09689 0.119679
Extraction group is 2. 0.145943 -0.02197 0.045139 0.080855
Extraction group is 3. 0.18139 0.152371 0.06198 0.12023
Extraction group is 4. 0.209632 0.137212 -0.03362 0.115398
Extraction group is 5. 0.15651 0.135912 -0.09021 0.302185
Extraction group is 6. 0.181983 0.148062 0.225597 0.239852
Extraction group is 7. 0.05339 0.196658 0.159718 0.141577
Extraction group is 8. 0.003744 0.03248 0.134471 0.118026
Camphor tree fallen leaves group 0.11917 0.027564 0.014439 0.050225
CK control group 0.222361 0.176106 0.151537 0.266455
Data in table 9 show that the Soil oxidation Asia of the 1st after preculture 2 days day, leaching liquor group is nitrogen discharged obviously low In camphor tree Litter leaf group and blank control group, nitrogen discharged, the significant effect that illustrates that leaching liquor can reduce rapidly Soil oxidation Asia.
10. Soil oxidation Asia nitrogen of table accumulates rate of discharge (μ gm-2)
Number of days 1 3 6 10 15
Extraction group is 1. 0.291447352 0.960523103 1.27954839 1.727859378 1.852991435
Extraction group is 2. 0.490200628 0.709128747 1.169476825 1.473794822 1.548051804
Extraction group is 3. 0.011839298 0.271018205 0.38534231 0.795592356 0.96912708
Extraction group is 4. -0.040067123 0.268037188 0.481766188 0.856936309 1.006029522
Extraction group is 5. -0.307044709 -0.097426969 0.389340546 0.633995827 0.854096573
Extraction group is 6. -0.344823089 0.128847731 0.335781143 0.740930967 0.942538108
Extraction group is 7. 0.408823114 0.714652811 0.972701474 1.16966049 1.412450869
Extraction group is 8. 0.231954172 0.608282793 0.755850178 1.095587172 1.198029596
Camphor tree fallen leaves group 2.331465789 2.496170488 2.70721516 2.936767565 3.07193053
CK control group 2.603148012 3.462231783 4.15261262 4.545160231 4.903744032
Number of days 21 28 35 42
Extraction group is 1. 1.795589205 1.961099202 2.057989305 2.177667938
Extraction group is 2. 1.693994465 1.672025361 1.717164451 1.798019228
Extraction group is 3. 1.150516995 1.302887914 1.364867628 1.485097255
Extraction group is 4. 1.21566139 1.352872922 1.319248185 1.434646021
Extraction group is 5. 1.010607008 1.146518759 1.056311219 1.358495882
Extraction group is 6. 1.1245214 1.272583266 1.49817982 1.738031567
Extraction group is 7. 1.465840735 1.662498823 1.822216324 1.963793419
Extraction group is 8. 1.201773526 1.234253892 1.368724583 1.486750235
Camphor tree fallen leaves group 3.191100554 3.218664361 3.233103219 3.283328404
CK control group 5.126105265 5.302211292 5.453748657 5.720203504
The nitrous oxide of camphor tree fallen leaves group and blank control group accumulation rate of discharge is consistently greater than camphortree leaf leaching liquor group, says Bright leaching liquor reduces the sub- nitrogen discharged significant effect of Soil oxidation.
The preparation method of camphortree leaf leaching liquor is simple, and raw material sources are wide, it is easy to promote, and is a kind of very economical reduction soil The method of earth nitrous oxide emission is significant.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto, In the technical scope disclosed by the present invention, any changes or substitutions that can be easily thought of by anyone skilled in the art, It should be covered by the protection scope of the present invention.

Claims (10)

1. a kind of Soil oxidation Asia nitrogen discharge-reducing method, which comprises the following steps:
Camphortree leaf is collected, is cleaned, is air-dried；
Air-dried camphortree leaf is taken, shreds to obtain the camphortree leaf of fragment shape, deionized water is added, then extracting at constant temperature is vibrated, is filtered, Camphortree leaf leaching liquor is obtained,
Camphortree leaf leaching liquor is applied to soil, monitors the discharge of Soil oxidation Asia nitrogen.
2. Soil oxidation Asia nitrogen discharge-reducing method as described in claim 1, which is characterized in that the camphortree leaf withers and falls for camphor tree Camphor tree Litter leaf after air-drying is shredded into length and width in the strip fragment of 2-3mm by leaf.
3. Soil oxidation Asia nitrogen discharge-reducing method as claimed in claim 1 or 2, which is characterized in that the camphortree leaf of fragment shape with plus The solid-to-liquid ratio of the deionized water entered is 1g:19mL.
4. Soil oxidation Asia nitrogen discharge-reducing method as claimed in claim 3, which is characterized in that the temperature of extracting at constant temperature is 35-50 DEG C, extraction time 1-2h.
5. Soil oxidation Asia nitrogen discharge-reducing method as claimed in claim 4, which is characterized in that the time of oscillation is 30-60min, The frequency of oscillation is 50-90Hz.
6. Soil oxidation Asia nitrogen discharge-reducing method as claimed in claim 5, which is characterized in that the temperature of extracting at constant temperature is 50 DEG C, Extraction time is 1h, and the frequency of oscillation is 80Hz, vibrates 30min.
7. a kind of camphortree leaf leaching liquor, which is characterized in that preparation process includes:
Camphor tree Litter leaf is collected, is cleaned, is air-dried, shreds to obtain length and width in the strip fragment of 2-3mm, deionization is added Water, 35-50 DEG C of extracting at constant temperature 1-2h, oscillation, filtering obtain the camphortree leaf leaching liquor.
8. a kind of camphortree leaf leaching liquor as claimed in claim 7, which is characterized in that the camphortree leaf of strip fragment in preparation process Solid-to-liquid ratio with the deionized water of addition is 1g:19mL.
9. a kind of camphortree leaf leaching liquor as claimed in claim 7 or 8, which is characterized in that the camphortree leaf leaching liquor is for dropping Greenhouse gas emission in low soil.
10. a kind of camphortree leaf leaching liquor as claimed in claim 9, which is characterized in that the camphortree leaf leaching liquor for reducing Nitrous oxide gas discharge in soil.
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103053240A (en) * 2012-12-04 2013-04-24 中国海洋大学 Method for utilizing bamboo reed charcoal to reduce soil nitrous oxide discharge
CN104381320A (en) * 2014-12-09 2015-03-04 张鹏 Non-polluted pesticide adopting camphor tree leaves as raw materials
CN106087385A (en) * 2016-07-08 2016-11-09 陆建益 A kind of Multifunctional woolen sweater finishing agent containing sea mud
CN106994342A (en) * 2017-05-05 2017-08-01 南昌工程学院 A kind of preparation method of photochemical catalyst
KR101762762B1 (en) * 2016-06-22 2017-08-07 농업회사법인 주식회사 자연과 미래 A method for producing a composition capable of simultaneously controlling pest insect pests containing an extract of camphor tree or pictorial tree as an effective ingredient, and a composition prepared by the method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103053240A (en) * 2012-12-04 2013-04-24 中国海洋大学 Method for utilizing bamboo reed charcoal to reduce soil nitrous oxide discharge
CN104381320A (en) * 2014-12-09 2015-03-04 张鹏 Non-polluted pesticide adopting camphor tree leaves as raw materials
KR101762762B1 (en) * 2016-06-22 2017-08-07 농업회사법인 주식회사 자연과 미래 A method for producing a composition capable of simultaneously controlling pest insect pests containing an extract of camphor tree or pictorial tree as an effective ingredient, and a composition prepared by the method
CN106087385A (en) * 2016-07-08 2016-11-09 陆建益 A kind of Multifunctional woolen sweater finishing agent containing sea mud
CN106994342A (en) * 2017-05-05 2017-08-01 南昌工程学院 A kind of preparation method of photochemical catalyst

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