CN110487221A - Hologram fringe central bright spot area and the micro- plastics detection device of dimension ratio and method - Google Patents
Hologram fringe central bright spot area and the micro- plastics detection device of dimension ratio and method Download PDFInfo
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- CN110487221A CN110487221A CN201910770989.7A CN201910770989A CN110487221A CN 110487221 A CN110487221 A CN 110487221A CN 201910770989 A CN201910770989 A CN 201910770989A CN 110487221 A CN110487221 A CN 110487221A
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- G01B11/00—Measuring arrangements characterised by the use of optical techniques
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- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
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Abstract
The present invention provides a kind of hologram fringe central bright spot area and the micro- plastics detection device of dimension ratio and method, the sequentially connected light source driving assembly of the device, light source, microporous element, light propagation component, sample stage components, image collection assembly, image processing modules.The light on and off of light source driving assembly control light emitting diode, sequential illumination feux rouges and green light, partially coherent light is diverged to spherical wave through microporous element by light, micro-fluidic chip is irradiated by light propagation component, sample stage component is reached after propagating a distance, it does not need to be introduced separately into reference light wave, the transmitted light of object is reference light wave, scattering light is Object light wave, reference light wave and Object light wave are interfered to be imaged by image collection assembly, holographic pattern is obtained, restores the three-dimensional appearance of sample by dual wavelength iterative algorithm.The method of the present invention is utilized respectively the ratio of the ratio and product of the central bright spot area of hologram fringe and the Length x Width of 3-D image and thickness summation to carry out the detection of micro- plastics.
Description
Technical field
The present invention relates to the technical fields of plastics micro- in a kind of pair of ocean detection, specifically, more particularly to hologram fringe
Central bright spot area and the micro- plastics detection device of dimension ratio and method
Background technique
According to the relevent statistics, nineteen sixty whole world plastic product output be 500,000 tons, and 2013 then close to 300,000,000 tons.Plastics
Product quality is light, and the rate of recovery is low, is not easily decomposed, and the sea leave of 60%-80% is modeling in the assessment of ocean shore-environment
Expect fragment.These plastic flakes gradually become fine particle under ultraviolet light, wind-force, wave friction, microbial action, pass through river
Stream, seabeach, marine mankind's activity and illegal dumping enter in seawater, huge to the eco-environmental impact of ocean.
U.S.National Oceanic and Atmospheric Administration (NOAA) are that diameter is broken less than 5mm to the definition of " micro- plastics " now
Piece.The source of micro- plastics includes terrestrial input, Seashore Tourist Trade, Shipping and mariculture fishing in the ocean being currently known
Deng.The input of Lu Yuan plastic garbage is the main source of plastic pollution in ocean, including is intentionally or accidentally abandoned in human lives
Plastic refuse, be washed by storm the plastic garbage buried the land of ocean, common some detergent, life shield
Skin product and the raw material of industry etc., plastics are difficult to remove in sewage disposal process since particle is small slightly for this, thus can be with land
Rubbish input in source enters ocean.These plastic garbages, which enter behind ocean, gradually occurs light degradation or broken, forms different-shape
Micro- plastics.
Micro- plastics are distributed widely in Marine fishery eco-environment waters, since its partial size is small, are easily taken in by fisheries biology, into
And a series of poisonous effects are generated, main includes inhibition growth and development, influence is ingested and capacity, genotoxicity, immune poison
Property, genetic damage etc..Micro- plastics are also possible to generate even more serious joint toxic effect in conjunction with other pollutants, and then influence
The stability of food chain in water body, and potential risks are come to the health care belt of the ecosystem.A large amount of micro- plastics swim in ocean
Surface is suspended in each layer seawater, seriously destroys the ornamental value of coastal beach, the development of impact on tourism, while ship
Navigation brings security risk.The toxic additive of micro- plastics inner is constantly discharged into seawater, while constantly being inhaled from seawater again
The toxic contaminants substance for receiving various hydrophobic, causes a kind of vicious circle.Micro- plastics are difficult to degrade, and form deposit in seabed,
And then the Biochemical processes that such as oxygen occurred on bottom sediment interface is exchanged with the diffusion of water are influenced, it seriously affects
Biological global chemical recycle.The micro- plastics in ocean swim in ocean surface, hinder the transmitting of light in the seawater, hinder sea-plant pair
The absorption of sunlight, and then its photosynthesis is influenced, oxygen and food can not be provided for marine ecosystems.The micro- plastics in ocean
It is balanced in the presence of marine ecology is seriously destroyed.The chemical toxicity of micro- plastics itself and the chemical toxicant adsorbed from environment, can
Directly injury can be generated to the biology ingested, and may be enriched in each trophic level organism along food chain direction,
And then threaten human health.Studies have shown that having had been found that micro- modeling in the animal body of each trophic level level of marine organisms chain
Material.The micro- plastics in ocean are also close at hand to the intuitive injury of the mankind.
Currently, micro- plastics identification method includes visual assessment method, Fourier transformation-infrared spectroscopy, Raman spectrum
Method, scanning electron microscope and the (such as scanning electron microscope-energy dispersion X-ray combination, environmental scanning electron microscope-energy color of energy disperse spectroscopy method
Dissipate X-ray combination, thermal desorption-gas chromatography-mass spectrometric hyphenated technique, hydrocarbon nitrogen analysis method etc.).The composition detection of micro- plastics is one
The more complicated work of item, since existing detection device is complicated, equipment is expensive, volume is big.So portable accurate detection is gone to sea
Micro- plastics of midocean are the emphasis studied at present.
Summary of the invention
According to the disadvantages of device of the existing detection micro- plastics in ocean set forth above is complicated, equipment is expensive, volume is big, and provide
A kind of portable accurate detection go to sea midocean micro- plastics hologram fringe central bright spot area and the micro- plastics of dimension ratio detect
Device and method.
The technological means that the present invention uses is as follows:
Hologram fringe central bright spot area and the micro- plastics detection device of dimension ratio, comprising: sequentially connected light source driving
Component, light source, microporous element, light propagation component, micro-fluidic chip, image collection assembly, image processing modules；
Described image acquisition component acquires the hologram image that micro-fluidic chip detection zone generates under light beam effect, described
Hologram image is sent to the image processing modules being connected with the micro-fluidic chip and carries out image analysis, obtains micro- plastics and is formed
Hologram image dimension ratio.
Further, the light source driving assembly is single-chip microcontroller, the light source is light emitting diode, and the single-chip microcontroller is automatic
The light on and off of light emitting diode, sequential illumination feux rouges and green light are controlled, light diverges to partially coherent light through the microporous element
Spherical wave irradiates the micro-fluidic chip by the light propagation component, reaches sample stage component after propagating a distance, is not required to
It is introduced separately into reference light wave, the transmitted light of object is reference light wave, and scattering light is Object light wave, and reference light wave and Object light wave are mutual
Interference is acquired by described image acquisition component and is imaged, and obtains hologram image.
Further, the micro-fluidic chip includes dimethyl silicone polymer piece and glass slide, the polydimethylsiloxanes
Successively intaglio has detection zone to alkane piece, and the detection zone both ends symmetrically connect sample cell (10).
Further, described image acquisition component is COMS imaging sensor；Described image processing component is also to micro- plastics
Sample carries out three-dimensional appearance recovery.
The present invention also provides a kind of hologram fringe central bright spot areas and the micro- plastics detection method of dimension ratio, by double
Wavelength iterative algorithm restores the three-dimensional appearance of sample, includes the following steps:
Step S1: red light wavelength is set as λ 1, and green wavelength is set as λ 2, and the complex amplitude of recording surface is counted at λ 1
Value simulation travels to sample plane；
Step S2: phase original phase is multiplied into 1/ λ 2 of λ, amplitude is constant；
Step S3: by updated sample plane wave wavefront to cmos image sensor plane back propagation at λ 2；
Step S4: the amplitude of the holographic pattern under amplitude green light is replaced；
Step S5: updated wavefront is traveled into sample plane in λ 2；
Step S6: amplitude is constant, and phase original phase multiplies 2/ λ 1 of λ；
Step S7: by updated sample plane wave wavefront to detector plane back propagation at λ 1；
Step S8: the amplitude substitution of the holographic pattern under amplitude feux rouges repeats step S1-S5 until reaching convergence shape
State.
Further, the method also includes obtaining the holographic pattern of sample using cmos image sensor, pass through three-dimensional
Pattern obtains the step of sample length, width and thickness information.
Further, the method also includes length, the width of central bright spot area and 3-D image using hologram fringe
The step of degree and the ratio of thickness summation detect micro- plastics.
Further, the method also includes length, the width of central bright spot area and 3-D image using hologram fringe
The step of degree and the ratio of thickness product detect micro- plastics.
Compared with the prior art, the invention has the following advantages that
1, micro- plastics detection device provided by the invention can get two dimension, the three-dimensional appearance of sample.Compared to optical microphotograph
Mirror, the present apparatus are suitble to the quick detection at scene, have the advantages that low price, light weight, small in size.
2, the method for the present invention detects micro- plastics in ocean without lens holographic technique using dual wavelength, compared to it
His hologram three-dimensional pattern technology, when restoring three-dimensional appearance, algorithm does not need unpacking step, enormously simplifies data processing
Step saves the time.
The present invention can be widely popularized in the fields such as micro- plastics detection in ocean based on the above reasons.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to do simply to introduce, it should be apparent that, the accompanying drawings in the following description is this hair
Bright some embodiments for those of ordinary skill in the art without any creative labor, can be with
It obtains other drawings based on these drawings.
Fig. 1 is the micro- plastics structure of the detecting device schematic diagram of the present invention；
Fig. 2 is microfluidic chip structure schematic diagram of the present invention；
Fig. 3 is dual wavelength iterative algorithm flow chart of steps of the present invention.
In figure: 1, light source driving assembly；2, light source；3, microporous element；4, light propagation component；5, micro-fluidic chip；6, scheme
As acquisition component；7, image processing modules；8, dimethyl silicone polymer piece；9, glass slide；10, sample cell；11, detection zone.
Specific embodiment
It should be noted that in the absence of conflict, the feature in embodiment and embodiment in the present invention can phase
Mutually combination.The present invention will be described in detail below with reference to the accompanying drawings and embodiments.
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is only
It is only a part of the embodiment of the present invention, instead of all the embodiments.It is real to the description of at least one exemplary embodiment below
It is merely illustrative on border, never as to the present invention and its application or any restrictions used.Based on the reality in the present invention
Example is applied, every other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to
In the scope of protection of the invention.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root
According to exemplary embodiments of the present invention.As used herein, unless the context clearly indicates otherwise, otherwise singular
Also it is intended to include plural form, additionally, it should be understood that, when in the present specification using term "comprising" and/or " packet
Include " when, indicate existing characteristics, step, operation, device, component and/or their combination.
Unless specifically stated otherwise, positioned opposite, the digital table of the component and step that otherwise illustrate in these embodiments
It is not limited the scope of the invention up to formula and numerical value.Simultaneously, it should be clear that for ease of description, each portion shown in attached drawing
The size divided not is to draw according to actual proportionate relationship.Technology known for person of ordinary skill in the relevant, side
Method and equipment may be not discussed in detail, but in the appropriate case, and the technology, method and apparatus should be considered as authorizing explanation
A part of book.In shown here and discussion all examples, appointing should be construed as merely illustratively to occurrence, and
Not by way of limitation.Therefore, the other examples of exemplary embodiment can have different values.It should also be noted that similar label
Similar terms are indicated in following attached drawing with letter, therefore, once it is defined in a certain Xiang Yi attached drawing, then subsequent attached
It does not need that it is further discussed in figure.
In the description of the present invention, it is to be understood that, the noun of locality such as " front, rear, top, and bottom, left and right ", " it is laterally, vertical,
Vertically, orientation or positional relationship indicated by level " and " top, bottom " etc. is normally based on orientation or position shown in the drawings and closes
System, is merely for convenience of description of the present invention and simplification of the description, in the absence of explanation to the contrary, these nouns of locality do not indicate that
It must have a particular orientation or be constructed and operated in a specific orientation with the device or element for implying signified, therefore cannot manage
Solution is limiting the scope of the invention: the noun of locality " inside and outside " refers to inside and outside the profile relative to each component itself.
For ease of description, spatially relative term can be used herein, as " ... on ", " ... top ",
" ... upper surface ", " above " etc., for describing such as a device shown in the figure or feature and other devices or spy
The spatial relation of sign.It should be understood that spatially relative term is intended to comprising the orientation in addition to device described in figure
Except different direction in use or operation.For example, being described as if the device in attached drawing is squeezed " in other devices
It will be positioned as " under other devices or construction after part or construction top " or the device of " on other devices or construction "
Side " or " under its device or construction ".Thus, exemplary term " ... top " may include " ... top " and
" in ... lower section " two kinds of orientation.The device can also be positioned with other different modes and (is rotated by 90 ° or in other orientation), and
And respective explanations are made to the opposite description in space used herein above.
In addition, it should be noted that, limiting components using the words such as " first ", " second ", it is only for be convenient for
Corresponding components are distinguished, do not have Stated otherwise such as, there is no particular meanings for above-mentioned word, therefore should not be understood as to this
The limitation of invention protection scope.
Since micro- plastics in ocean are different from the hologram image that other samples are formed, the holographic pattern feature formed is just
Difference, according to above-mentioned principle, the present invention relates to micro- modelings based on hologram fringe central bright spot area Yu 3-D image dimension ratio
Expect detection device and method, the present invention is further described with reference to the accompanying drawing.
As shown in Figure 1, the present invention provides hologram fringe central bright spot area and the micro- plastics detection device of dimension ratio, packet
It includes: sequentially connected light source driving assembly 1, light source 2, microporous element 3, light propagation component 4, micro-fluidic chip 5, Image Acquisition group
Part 6, image processing modules 7.Light source driving assembly 1 is single-chip microcontroller, light source 2 is light emitting diode, and Automatic control of single chip microcomputer shines
Partially coherent light is diverged to spherical wave through microporous element 3 by the light on and off of diode, sequential illumination feux rouges and green light, light, is passed through
The light propagation component 4 irradiates micro-fluidic chip 5, reaches sample stage component after propagating a distance, does not need to be introduced separately into ginseng
Light wave is examined, the transmitted light of object is reference light wave, and scattering light is Object light wave, and reference light wave and Object light wave are interfered and adopted by image
Collect component 6 (COMS imaging sensor) acquisition imaging, obtains hologram image.Hologram image is sent to be connected with micro-fluidic chip 5
The image processing modules 7 connect carry out image analysis, obtain the hologram image dimension ratio that micro- plastics are formed.Pass through dual wavelength iteration
Algorithm restores the three-dimensional appearance of sample, obtains sample length, width and thickness information by three-dimensional appearance.
As shown in Fig. 2, micro-fluidic chip 5 includes dimethyl silicone polymer piece 8 and glass slide 9, dimethyl silicone polymer piece 8
Successively intaglio has detection zone 11, and 11 both ends of detection zone symmetrically connect sample cell 10.
As shown in figure 3, the present invention also provides hologram fringe central bright spot area and the micro- plastics detection method of dimension ratio,
The three-dimensional appearance for restoring sample by dual wavelength iterative algorithm, includes the following steps:
Step S1: red light wavelength is set as λ 1, and green wavelength is set as λ 2, and the complex amplitude of recording surface is counted at λ 1
Value simulation travels to sample plane；
Step S2: phase original phase is multiplied into 1/ λ 2 of λ, amplitude is constant；
Step S3: by updated sample plane wave wavefront to cmos image sensor plane back propagation at λ 2；
Step S4: the amplitude of the holographic pattern under amplitude green light is replaced；
Step S5: updated wavefront is traveled into sample plane in λ 2；
Step S6: amplitude is constant, and phase original phase multiplies 2/ λ 1 of λ；
Step S7: by updated sample plane wave wavefront to detector plane back propagation at λ 1；
Step S8: the amplitude substitution of the holographic pattern under amplitude feux rouges repeats step S1-S5 until reaching convergence shape
State.
Embodiment 1
Using the central bright spot area and the length of 3-D image of hologram fringe, width and thickness summation ratio to micro-
Plastics are detected；The length of the size difference central bright spot areas of micro- plastics and 3-D image, width and thickness summation
Ratio is different.
Embodiment 2
Using the central bright spot area and the length of 3-D image of hologram fringe, width and thickness product ratio to micro-
Plastics are detected；The length of the size difference central bright spot areas of micro- plastics and 3-D image, width and thickness product
Ratio is different.
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: its according to
So be possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features into
Row equivalent replacement；And these are modified or replaceed, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme.
Claims (8)
1. a kind of hologram fringe central bright spot area and the micro- plastics detection device of dimension ratio, which is characterized in that described device packet
Include: sequentially connected light source driving assembly (1), light source (2), microporous element (3), light propagation component (4), micro-fluidic chip (5),
Image collection assembly (6), image processing modules (7)；
Described image acquisition component (6) acquires the hologram that micro-fluidic chip (5) detection zone (11) generate under light beam effect
Picture, the hologram image are sent to the image processing modules (7) being connected with the micro-fluidic chip (5) and carry out image analysis,
Obtain the hologram image dimension ratio that micro- plastics are formed.
2. hologram fringe central bright spot area according to claim 1 and the micro- plastics detection device of dimension ratio, feature
It is, the light source driving assembly (1) is single-chip microcontroller, the light source (2) is light emitting diode, the Automatic control of single chip microcomputer hair
Partially coherent light is diverged to spherical surface through the microporous element (3) by the light on and off of optical diode, sequential illumination feux rouges and green light, light
Wave irradiates the micro-fluidic chip (5) by the light propagation component (4), reaches sample stage component after propagating a distance, no
Need to be introduced separately into reference light wave, the transmitted light of object is reference light wave, and scattering light is Object light wave, reference light wave and Object light wave phase
Mutually interference is acquired by described image acquisition component (6) and is imaged, and obtains hologram image.
3. hologram fringe central bright spot area according to claim 1 or 2 and the micro- plastics detection device of dimension ratio, special
Sign is that the micro-fluidic chip (5) includes dimethyl silicone polymer piece (8) and glass slide (9), the dimethyl silicone polymer
Successively intaglio has detection zone (11) piece (8), and detection zone (11) both ends symmetrically connect sample cell (10).
4. hologram fringe central bright spot area according to claim 1 and the micro- plastics detection device of dimension ratio, feature
It is, described image acquisition component (6) is COMS imaging sensor；Described image processing component (7) also to micro- plastic sample into
Row three-dimensional appearance restores.
5. a kind of hologram fringe central bright spot area and the micro- plastics detection method of dimension ratio, which is characterized in that pass through dual wavelength
Iterative algorithm restores the three-dimensional appearance of sample, includes the following steps:
Step S1: red light wavelength is set as λ 1, and green wavelength is set as λ 2, and the complex amplitude of recording surface is carried out Numerical-Mode at λ 1
It is quasi- to travel to sample plane；
Step S2: phase original phase is multiplied into 1/ λ 2 of λ, amplitude is constant；
Step S3: by updated sample plane wave wavefront to cmos image sensor plane back propagation at λ 2；
Step S4: the amplitude of the holographic pattern under amplitude green light is replaced；
Step S5: updated wavefront is traveled into sample plane in λ 2；
Step S6: amplitude is constant, and phase original phase multiplies 2/ λ 1 of λ；
Step S7: by updated sample plane wave wavefront to detector plane back propagation at λ 1；
Step S8: the amplitude substitution of the holographic pattern under amplitude feux rouges repeats step S1-S5 until reaching convergence state.
6. hologram fringe central bright spot area according to claim 5 and the micro- plastics detection method of dimension ratio, feature
It is, the method also includes obtaining the holographic pattern of sample using cmos image sensor, sample is obtained by three-dimensional appearance
The step of length, width and thickness information.
7. hologram fringe central bright spot area according to claim 5 and the micro- plastics detection method of dimension ratio, feature
It is, the method also includes the length of central bright spot area and 3-D image using hologram fringe, width and thickness are total
The step of ratio of sum detects micro- plastics.
8. hologram fringe central bright spot area according to claim 5 and the micro- plastics detection method of dimension ratio, feature
It is, the method also includes being multiplied using the central bright spot area of hologram fringe and length, width and the thickness of 3-D image
The step of long-pending ratio detects micro- plastics.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112098277A (en) * | 2020-09-15 | 2020-12-18 | 大连海事大学 | Method for detecting type and activity of high-concentration microparticles based on three-wavelength lens-free holographic imaging |
| CN114354531A (en) * | 2021-12-30 | 2022-04-15 | 合肥工业大学 | Plastic identification system of double-wavelength coherent light source based on near infrared |
| CN114570495A (en) * | 2020-11-17 | 2022-06-03 | 株式会社希力卡·装备Nq | Analysis device |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205538736U (en) * | 2015-12-30 | 2016-08-31 | 中国工程物理研究院激光聚变研究中心 | Optical element surface defect detecting device of transmission dual wavelength synthetic aperture holography |
| CN105974765A (en) * | 2016-05-02 | 2016-09-28 | 浙江大学 | Portable digital holographic microscopy |
| CN107101943A (en) * | 2017-05-18 | 2017-08-29 | 大连海事大学 | A kind of optofluidic is without lens holographic imaging activity of microalgae detection means and method |
| US10048201B2 (en) * | 2012-09-10 | 2018-08-14 | The Trustees Of Princeton University | Fluid channels for computational imaging in optofluidic microscopes |
| CN108489910A (en) * | 2018-03-09 | 2018-09-04 | 大连理工大学 | Micro- plastics rapid detection method in a kind of Oysters based on hyperspectral technique |
| CN109852541A (en) * | 2018-12-09 | 2019-06-07 | 大连海事大学 | Cytoactive detection apparatus and method based on the total gray value of hologram fringe Yu gray scale difference ratio |
-
2019
- 2019-08-20 CN CN201910770989.7A patent/CN110487221B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10048201B2 (en) * | 2012-09-10 | 2018-08-14 | The Trustees Of Princeton University | Fluid channels for computational imaging in optofluidic microscopes |
| CN205538736U (en) * | 2015-12-30 | 2016-08-31 | 中国工程物理研究院激光聚变研究中心 | Optical element surface defect detecting device of transmission dual wavelength synthetic aperture holography |
| CN105974765A (en) * | 2016-05-02 | 2016-09-28 | 浙江大学 | Portable digital holographic microscopy |
| CN107101943A (en) * | 2017-05-18 | 2017-08-29 | 大连海事大学 | A kind of optofluidic is without lens holographic imaging activity of microalgae detection means and method |
| CN108489910A (en) * | 2018-03-09 | 2018-09-04 | 大连理工大学 | Micro- plastics rapid detection method in a kind of Oysters based on hyperspectral technique |
| CN109852541A (en) * | 2018-12-09 | 2019-06-07 | 大连海事大学 | Cytoactive detection apparatus and method based on the total gray value of hologram fringe Yu gray scale difference ratio |
Non-Patent Citations (2)
| Title |
|---|
| 中国感光学会: "《2016-2017感光影像学学科发展报告》", 31 March 2018, 中国科学技术出版社 * |
| 赵益乐: "基于无透镜数字全息的微颗粒成像系统设计", 《中国优秀硕士学位论文全文数据库 信息科技辑》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112098277A (en) * | 2020-09-15 | 2020-12-18 | 大连海事大学 | Method for detecting type and activity of high-concentration microparticles based on three-wavelength lens-free holographic imaging |
| CN114570495A (en) * | 2020-11-17 | 2022-06-03 | 株式会社希力卡·装备Nq | Analysis device |
| CN114570495B (en) * | 2020-11-17 | 2023-08-29 | 株式会社新科集团 | Analysis device |
| CN114354531A (en) * | 2021-12-30 | 2022-04-15 | 合肥工业大学 | Plastic identification system of double-wavelength coherent light source based on near infrared |
| CN114354531B (en) * | 2021-12-30 | 2024-02-02 | 合肥工业大学 | Plastic identification system of dual-wavelength coherent light source based on near infrared |
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