CN110484434A - Micro-fluidic chip, based on micro-fluidic cell sorting method and system - Google Patents
Micro-fluidic chip, based on micro-fluidic cell sorting method and system Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000001914 filtration Methods 0.000 claims abstract description 26
- 239000000758 substrate Substances 0.000 claims abstract description 10
- 239000012535 impurity Substances 0.000 claims abstract description 7
- 238000012216 screening Methods 0.000 claims abstract description 4
- 239000000872 buffer Substances 0.000 claims description 21
- 239000007788 liquid Substances 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 4
- 239000012530 fluid Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 2
- 239000004205 dimethyl polysiloxane Substances 0.000 description 2
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000010191 image analysis Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 2
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 2
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229920005573 silicon-containing polymer Polymers 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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Abstract
The present invention relates to a kind of micro-fluidic chip, based on micro-fluidic cell sorting system and method, the micro-fluidic chip includes substrate, the substrate includes filtering area, Image Acquisition channel and sorter, the filtering area is provided with the filter for impurity screening, the sorter includes at least two collection channels, each collection channel acquires channel by described image and is connected to filtering area, and described image acquisition channel only allows unicellular stream to pass through.The method of the present invention is the image of direct acquisition individual cells, then the size of cell is obtained by image procossing, finally by different size of cell sorting to different collectors.The sorting of the different size of cell of one species not only may be implemented in the present invention, but also accuracy is higher, and system structure is more simplified.
Description
Technical field
The present invention relates to cell processing techniques field, in particular to a kind of micro-fluidic chip is divided based on micro-fluidic cell
Choosing method and system.
Background technique
It is essentially all at present using flow cytometer for cell screening.Flow cytometer is to utilize fluorescent marker side
Formula screens the cell with fluorescence from cell mass.It, can be to same since same kind of cell has identical characteristic
Fluorescence reacts, therefore this mode can only filter out same kind of cell from different types of cell, and cannot be to same
The cell of one type is screened.
The patent application of Chinese Publication No. CN109863384A disclose a kind of cell sorting system based on image and
Method, this method also utilize laser excitation cell (fluorescent staining/be unstained) and shine, and photomultiplier tube goes detection strong light
Degree reconstructs the figure of cell according to luminous intensity, is then sorted, this method according to the property of cell (size, profile etc.)
The cell that specified size is filtered out from same cells may be implemented, but there is also defects for this method.For example, this method is both used
It has arrived fluorescent marker and image procossing is utilized again, system structure is relatively complicated；In another example this method is using detecting
The image that voltage reconfigures, and reconstructed according to cell light intensity, therefore, the accuracy of reconstructed image is difficult to ensure.
Summary of the invention
It is an object of the invention to improve the deficiency that structure is complicated, accuracy is not high in the presence of the prior art, provide
A kind of micro-fluidic chip, and based on micro-fluidic cell sorting method and system.
In order to achieve the above-mentioned object of the invention, the embodiment of the invention provides following technical schemes:
A kind of micro-fluidic chip, including substrate, the substrate include filtering area, Image Acquisition channel and sorter, described
Filtering area is provided with the filter for impurity screening, and the sorter includes at least two collection channels, each collection channel
It acquires channel by described image to be connected to filtering area, described image acquisition channel only allows unicellular stream to pass through.
In above structure, by the way that Image Acquisition channel is arranged, and Image Acquisition channel only allows unicellular stream to pass through, can be with
It realizes the image for acquiring individual cells when individual cells pass through Image Acquisition channel, then realizes and obtained carefully by image analysis
The size of born of the same parents, and cell is sorted according to cell size.
In the scheme advanced optimized, the substrate further includes buffer area, and the filtering area is acquired by described image
Channel is connected to the buffer area, and the buffer area passes through only the focusing channel for allowing unicellular stream to pass through again and described collect is led to
Road connection.By setting buffer area, the cell quickly flowed in Image Acquisition channel is allowed to reduce in buffer area,
Then the enough reaction time is provided for data processing unit and suction unit, then ensures each cell by effectively and accurately
Ground is drawn into corresponding collector.
On the other hand, the embodiment of the invention also provides a kind of based on micro-fluidic cell sorting system, including this implementation
Micro-fluidic chip described in example any embodiment, further including includes driver, microscope, camera, data processing unit and pumping
Inhale device；The driver is for driving cell liquid to flow in micro-fluidic chip, and microscope is for amplifying in micro-fluidic chip
Image Acquisition channel in the cell that circulates, camera obtains each cell for taking pictures to amplified individual cells
Cell image, data processing unit is used to extract the diameter parameters of cell in the cell image of acquisition；The suction unit
For different size of cell to be pumped in corresponding collector under the control of data processing unit.
On the other hand, the embodiment of the invention also provides a kind of based on micro-fluidic cell sorting method, including following step
It is rapid:
Step 1, the image of individual cells in Image Acquisition channel is successively acquired；
Step 2, image procossing is carried out to the image of the individual cells of acquisition, extracts the diameter parameters of the cell；
Step 3, according to the diameter parameters of obtained cell, start corresponding suction unit, will will currently enter sorter
Cell be pumped in the collector of corresponding size.
Compared with prior art, various sizes of cell point in same cells not only may be implemented in present system and method
Choosing, and be that cell size identification and sorting directly are carried out to the cell image of acquisition, reduce fluorescence mark in system structure
The structure of note, then simplifies system structure, reduces system cost, the process of cell reconstitution is reduced in processing method, no
Method flow is only simplified, the efficiency of separation is improved, and eliminates the error that cell reconstitution may cause, that is, improves cell
The accuracy of sorting.In short, the structure of this system is simpler, method operation is easier, and more efficient, accuracy is higher.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair
The restriction of range for those of ordinary skill in the art without creative efforts, can also be according to this
A little attached drawings obtain other relevant attached drawings.
Fig. 1 is the structural schematic diagram based on micro-fluidic cell sorting system in embodiment.
Fig. 2 is the structural schematic diagram of micro-fluidic chip in embodiment.
Fig. 3 is the flow chart based on micro-fluidic cell sorting method in embodiment.
Marked in the figure: 1- filtering area, 2- Image Acquisition channel, the buffer area 3-, 4- focuses channel, 11- locating piece, 12- the
One support column, the second support column of 13-, 14- filter, 51- collection channel, the entrance of 52- pump.
Specific embodiment
Below in conjunction with attached drawing in the embodiment of the present invention, technical solution in the embodiment of the present invention carries out clear, complete
Ground description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Usually exist
The component of the embodiment of the present invention described and illustrated in attached drawing can be arranged and be designed with a variety of different configurations herein.Cause
This, is not intended to limit claimed invention to the detailed description of the embodiment of the present invention provided in the accompanying drawings below
Range, but it is merely representative of selected embodiment of the invention.Based on the embodiment of the present invention, those skilled in the art are not doing
Every other embodiment obtained under the premise of creative work out, shall fall within the protection scope of the present invention.
Fig. 1 show company provided in this embodiment based on micro-fluidic cell sorting system, in figure between each box
Line does not indicate that connection/connected relation, dotted line with the arrow indicate signal connection.Referring to Fig. 1, schematically being mentioned in the present embodiment
It has supplied a kind of based on micro-fluidic cell sorting system, including driver, micro-fluidic chip, microscope, camera, data processing list
Member and pump.Wherein, driver uses constant pressure pump or peristaltic pump, and the cell liquid in sample bottle is driven into micro-fluidic chip, and
(walking) is flowed in micro-fluidic chip；Micro-fluidic chip provides unicellular circulation road, so that cell liquid is in a manner of unicellular stream
It circulates on micro-fluidic chip, unicellular stream refers to that one cell of primary only permission passes through；Microscope is used in cell micro-
When circulating in fluidic chip, amplify individual cells；Camera is using ultrahigh speed video camera (such as U.S. Phantom V6, Japan
Photron etc.), for taking pictures to the individual cells amplified under microscope, obtain the image of each individual cells；Data
Processing unit is then used to carry out processing analysis to the cell image that camera acquires, and acquires the diameter (size) of each cell；Pump is then
For different size of cell to be pumped in corresponding collector, pump can also be replaced using solenoid valve.Data processing unit
Programmable logic device FPGA or micro-control unit MCU etc. can be used.
More specifically, the structure of above-mentioned micro-fluidic chip is as shown in Figure 2 in the present embodiment.Referring to Fig. 2, micro-fluidic chip
Including filter 14, Image Acquisition channel 2, focus channel 4 and sorter.The cell liquid come out from sample bottle initially enters filtering
In device 14, the contaminant filter in cell liquid is fallen using filter 14, only cell and tissue fluid is allowed to pass through, into Image Acquisition
Channel 2 avoids impurity from blocking Image Acquisition channel 2.In the present embodiment, Image Acquisition channel 2 is that a diameter is about 10-
The channel of 15um, effect are only individual cells to be allowed to pass through.It is readily comprehensible, according to the of different sizes of detected cell, figure
As the diameter of acquisition channel 2 accordingly changes.When unicellular stream is by Image Acquisition channel 2, camera is i.e. to each passed through
Individual cells are taken pictures, and one or more image of the individual cells is obtained.What is come out from Image Acquisition channel 2 is unicellular
Stream continues to flow to buffer area 3, then flows to again and focuses channel 4, and focusing channel 4 is also the ditch for only individual cells being allowed to pass through
Road, it is therefore an objective to allow and unicellular stream is still maintained by the cell after Image Acquisition channel 2, it is ensured that sorting, which exports the cell sorted, is
The cell detected.Sorter includes at least two collection channels, and a collection channel is connected with a pump or solenoid valve, different
The cell of size is pumped respectively to be drawn onto corresponding collector.
More specifically, micro-fluidic chip includes PDMS (dimethyl silicone polymer) substrate and coverslip, PDMS substrate include
Filtering area 1, buffer area 3 and sorting area, cell and tissue fluid can walk in filtering area 1, buffer area 3 and sorting area.Filter
14 are set to filtering area 1, and filter 14 includes that several are in the pillar of array distribution, interval and image between pillar and pillar
Acquire the of same size of channel 2, that is, the gap between pillar and pillar forms filter screen, only allows individual cells and tissue fluid
Pass through, larger sized impurity is then stopped by pillar and must not enter Image Acquisition channel 2.Fig. 2 show the setting of filter 14
In the middle position of filtering area 1, it is readily understood that, this is only preferred embodiment, and filter 14 also can be set in filtering area 1
Other positions.
Please continue to refer to Fig. 2, in order to receive the cell and tissue fluid that come out from sample bottle, so filtering area 1 is relatively wide,
Collapse phenomenon occurs in order to prevent filter 14, is provided with support column in filtering area 1.In structure shown in Fig. 2, support column includes
Several first support columns 12 and several second support columns 13, the first support column 12 are rectangle rod structure, are set to filter
14 front, the second support column 13 are cylindrical structure, are set to the rear of filter 14.It is also to be understood that the first support column
12 not only have supporting role, also have filtration, after the filtering of the first support column 12, filter out bigger impurity, smaller
Impurity then pass through filter 14 and filter.Based on identical consideration, buffer area 3 is also equipped with several the second support columns 13.
Filtering area 1 and buffer area 3 be connected tos by Image Acquisition channel 2, the cell being photographed pass through Image Acquisition channel 2 into
Enter buffer area 3.Due to cell by Image Acquisition channel 2 speed it is opposite be than faster, and FPGA carry out image procossing with
And corresponding pump (or solenoid valve) work of control needs the time, therefore in order to FPGA and the reserved reaction of pump (or solenoid valve)
Time is provided with buffer area 3 in the present embodiment, speed can be fallen in buffer area 3 by the cell of Image Acquisition channel 2
Come.It is readily comprehensible, if the reaction speed of FPGA and pump is sufficiently fast, buffer area 3 can also be not provided with and cached, accordingly
Ground focuses channel 4 without setting.
Buffer area is connected to sorting area by focusing channel 4, and the cell that speed lowers in buffer area 3 is by focusing channel
4 enter sorting area.Due to focus channel 4 be also it is very narrow, only allow an individual cells pass through, therefore, when cell by focus channel 4
Into after sorting area, FPGA controls corresponding pump work.
Sorter include at least two collection channels 51, each collection channel 51 it is of different size, it is various sizes of to allow
Cell passes through, and one end of collection channel 51 is connected to channel 4 is focused, and the other end is connected to the entrance 52 of pump, and a pump is corresponding to be connected
A collector is connect, the cell of correspondingly-sized is pumped and is drawn in collector after pump startup.
Micro-fluidic chip is placed on microscopical objective table, since micro-fluidic chip is smaller, for the ease of looking for
To the edge of device, in structure shown in Fig. 2, positioning is provided on the outside of the edge of filtering area 1 and on the outside of the edge in sorting area
Part 11, such as positioning column, positioning column not only have positioning/marked effect, but also are also used as decoration with beautification function.
Based on above-mentioned cell sorting system, the present embodiment provides a kind of cell sorting method simultaneously.As shown in figure 3, this
Embodiment provide based on micro-fluidic cell sorting method the following steps are included:
Step 1, the image of individual cells is acquired.Since the cell of extraction is to be present in tissue fluid in a cluster, need
It will be by means of micro-fluidic chip shown in Fig. 2, so that cell individually individually passes through Image Acquisition channel 2, in order to realize pair
Individual cells carry out Image Acquisition.Micro-fluidic chip is directed at Image Acquisition channel 2, high speed camera on microscopical objective table
It takes pictures to the region of object lens amplification.In image acquisition process, camera is in normally open, i.e. phase chance continuously
It takes pictures, one or more image may be collected for the same cell, turn off camera after to be detected.
Step 2, image procossing is carried out to the image of the individual cells of acquisition, such as first converts collected cell image
For grayscale image, then grayscale image is enhanced, is filtered, the edge of cell is then extracted, finally according to the edge of cell
The parameters such as the size of contours extract cell and diameter.For cell image analysis processing using prior art means, because
This is described only briefly herein, or can also be based on reference to one kind of the patent application publication of Publication No. CN109863384A
Corresponding description in the cell sorting system and method for image.
Step 3, according to the size of each cell obtained after image procossing, different size of cell is pumped to pair respectively
It answers in the collector of size.Based on above system, FPGA is to the cell for obtaining being currently entering sorting area after cell image processing
Then parameter, such as diameter export control signal to corresponding pump (or solenoid valve), starting pump according to the size of cell
(or solenoid valve) extracts target cell, realizes different size of cell sorting.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.
Claims (9)
1. a kind of micro-fluidic chip, including substrate, which is characterized in that the substrate includes filtering area, Image Acquisition channel and divides
Device is selected, the filtering area is provided with the filter for impurity screening, and the sorter includes at least two collection channels, each
Collection channel acquires channel by described image and is connected to filtering area, and described image acquisition channel only allows unicellular stream to pass through.
2. micro-fluidic chip according to claim 1, which is characterized in that the substrate further includes buffer area, the filtering
Area acquires channel by described image and is connected to the buffer area, and the buffer area passes through only gathering of allowing unicellular stream to pass through again
Burnt channel is connected to the collection channel.
3. micro-fluidic chip according to claim 2, which is characterized in that the filtering area and/or setting buffers have branch
Dagger.
4. micro-fluidic chip according to claim 1, which is characterized in that be provided with and be used on the outside of the edge of the filtering area
The locating piece of location mark, and/or, so being provided with the locating piece for location mark on the outside of the edge in sorting area.
5. micro-fluidic chip according to claim 1, which is characterized in that the filter includes several in array distribution
Pillar, the interval between pillar is equal to the width of Image Acquisition channel.
6. a kind of based on micro-fluidic cell sorting system, which is characterized in that any described including driver, claim 1-5
Micro-fluidic chip, microscope, camera, data processing unit and suction unit；The driver is for driving cell liquid micro-
It is flowed in fluidic chip, for amplifying the cell to circulate in the Image Acquisition channel of micro-fluidic chip, camera is used for microscope
It takes pictures to amplified individual cells, obtains the cell image of each cell, data processing unit is adopted for extracting
The diameter parameters of cell in the cell image of collection；The suction unit is used for different size under the control of data processing unit
Cell be pumped in corresponding collector.
7. according to claim 6 based on micro-fluidic cell sorting system, which is characterized in that the suction unit is pump
Or solenoid valve.
8. a kind of based on micro-fluidic cell sorting method, which comprises the following steps:
Step 1, the image of individual cells in Image Acquisition channel is successively acquired；
Step 2, image procossing is carried out to the image of the individual cells of acquisition, extracts the diameter parameters of the cell；
Step 3, according to the diameter parameters of obtained cell, start corresponding suction unit, will will currently enter the thin of sorter
Born of the same parents are pumped in the collector of corresponding size.
9. according to the method described in claim 8, it is characterized in that, in the step 1, when individual cells pass through Image Acquisition ditch
When road, the cell image of the individual cells is acquired under microscopical booster action.
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| CN201910732392.3A CN110484434A (en) | 2019-08-09 | 2019-08-09 | Micro-fluidic chip, based on micro-fluidic cell sorting method and system |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101801491A (en) * | 2007-09-13 | 2010-08-11 | 阿尔利克斯公司 | Methods and apparatuses for sorting objects in forensic DNA analysis and medical diagnostics |
| CN106076441A (en) * | 2016-06-07 | 2016-11-09 | 中国科学院上海微系统与信息技术研究所 | A kind of micro fluidic device based on size detection circulating tumor cell and method |
| CN109863384A (en) * | 2016-06-10 | 2019-06-07 | 加利福尼亚大学董事会 | Cell sorting system and method based on image |
| CN109943475A (en) * | 2019-04-12 | 2019-06-28 | 广西医科大学第一附属医院 | The micro-fluidic sorting chip of one kind and its separation system |
| CN109967143A (en) * | 2019-02-27 | 2019-07-05 | 西安理工大学 | A kind of cell size detection method based on micro-fluidic microscopic system |
-
2019
- 2019-08-09 CN CN201910732392.3A patent/CN110484434A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101801491A (en) * | 2007-09-13 | 2010-08-11 | 阿尔利克斯公司 | Methods and apparatuses for sorting objects in forensic DNA analysis and medical diagnostics |
| CN106076441A (en) * | 2016-06-07 | 2016-11-09 | 中国科学院上海微系统与信息技术研究所 | A kind of micro fluidic device based on size detection circulating tumor cell and method |
| CN109863384A (en) * | 2016-06-10 | 2019-06-07 | 加利福尼亚大学董事会 | Cell sorting system and method based on image |
| CN109967143A (en) * | 2019-02-27 | 2019-07-05 | 西安理工大学 | A kind of cell size detection method based on micro-fluidic microscopic system |
| CN109943475A (en) * | 2019-04-12 | 2019-06-28 | 广西医科大学第一附属医院 | The micro-fluidic sorting chip of one kind and its separation system |
Non-Patent Citations (1)
| Title |
|---|
| 王立鼎，刘冲，徐征, 国防工业出版社 * |
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Application publication date: 20191122 |