CN110478499A - A kind of preparation method of BiOI- protein composite nano plate CT images probe - Google Patents
A kind of preparation method of BiOI- protein composite nano plate CT images probe Download PDFInfo
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- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 69
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 69
- 239000002131 composite material Substances 0.000 title claims abstract description 19
- 239000002055 nanoplate Substances 0.000 title claims abstract description 19
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 239000000523 sample Substances 0.000 title claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000000594 mannitol Substances 0.000 claims abstract description 12
- 230000007062 hydrolysis Effects 0.000 claims abstract description 3
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 3
- 229910001451 bismuth ion Inorganic materials 0.000 claims description 27
- 239000000243 solution Substances 0.000 claims description 22
- 238000005406 washing Methods 0.000 claims description 14
- 239000007864 aqueous solution Substances 0.000 claims description 13
- 239000011259 mixed solution Substances 0.000 claims description 12
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 11
- 229930195725 Mannitol Natural products 0.000 claims description 11
- 235000010355 mannitol Nutrition 0.000 claims description 11
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims description 9
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 9
- 229940098773 bovine serum albumin Drugs 0.000 claims description 9
- 239000008367 deionised water Substances 0.000 claims description 9
- 229910021641 deionized water Inorganic materials 0.000 claims description 9
- 229910052740 iodine Inorganic materials 0.000 claims description 9
- 239000011630 iodine Substances 0.000 claims description 9
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 claims description 9
- 238000001291 vacuum drying Methods 0.000 claims description 8
- 238000005119 centrifugation Methods 0.000 claims description 7
- 239000003153 chemical reaction reagent Substances 0.000 claims description 7
- FVAUCKIRQBBSSJ-UHFFFAOYSA-M sodium iodide Chemical compound [Na+].[I-] FVAUCKIRQBBSSJ-UHFFFAOYSA-M 0.000 claims description 6
- 108010088751 Albumins Proteins 0.000 claims description 4
- 102000009027 Albumins Human genes 0.000 claims description 4
- 102000001554 Hemoglobins Human genes 0.000 claims description 4
- 108010054147 Hemoglobins Proteins 0.000 claims description 4
- 210000002966 serum Anatomy 0.000 claims description 4
- XZXYQEHISUMZAT-UHFFFAOYSA-N 2-[(2-hydroxy-5-methylphenyl)methyl]-4-methylphenol Chemical compound CC1=CC=C(O)C(CC=2C(=CC=C(C)C=2)O)=C1 XZXYQEHISUMZAT-UHFFFAOYSA-N 0.000 claims description 2
- 241000283690 Bos taurus Species 0.000 claims description 2
- 229940107816 ammonium iodide Drugs 0.000 claims description 2
- 235000009518 sodium iodide Nutrition 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 6
- 238000005265 energy consumption Methods 0.000 abstract description 3
- 238000006243 chemical reaction Methods 0.000 abstract description 2
- 238000003756 stirring Methods 0.000 description 20
- 239000002872 contrast media Substances 0.000 description 9
- BDJYZEWQEALFKK-UHFFFAOYSA-N bismuth;hydrate Chemical compound O.[Bi] BDJYZEWQEALFKK-UHFFFAOYSA-N 0.000 description 7
- XMBWDFGMSWQBCA-UHFFFAOYSA-M iodide Chemical compound [I-] XMBWDFGMSWQBCA-UHFFFAOYSA-M 0.000 description 7
- 229940006461 iodide ion Drugs 0.000 description 7
- 239000002245 particle Substances 0.000 description 7
- 238000003384 imaging method Methods 0.000 description 6
- 230000006872 improvement Effects 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 238000013170 computed tomography imaging Methods 0.000 description 5
- LELOWRISYMNNSU-UHFFFAOYSA-N hydrogen cyanide Chemical compound N#C LELOWRISYMNNSU-UHFFFAOYSA-N 0.000 description 4
- 239000002086 nanomaterial Substances 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 3
- 239000002105 nanoparticle Substances 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000001788 irregular Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000000634 powder X-ray diffraction Methods 0.000 description 2
- 210000004872 soft tissue Anatomy 0.000 description 2
- 206010029155 Nephropathy toxic Diseases 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052797 bismuth Inorganic materials 0.000 description 1
- JCXGWMGPZLAOME-UHFFFAOYSA-N bismuth atom Chemical compound [Bi] JCXGWMGPZLAOME-UHFFFAOYSA-N 0.000 description 1
- 150000001622 bismuth compounds Chemical class 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 238000002591 computed tomography Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000007694 nephrotoxicity Effects 0.000 description 1
- 231100000417 nephrotoxicity Toxicity 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/04—X-ray contrast preparations
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- Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a kind of preparation methods of BiOI- protein composite nano plate CT images probe, inhibit Bi by addition mannitol3+Hydrolysis, BiOI- protein composite nano plate has been made in water phase at room temperature.The BiOI- protein composite nano plate CT images probe that the present invention prepares has reaction condition mild, the features such as large specific surface area, pattern are uniform, size is controllable, bio-toxicity is low, can be used as CT images probe, in terms of have potential application prospect.It is a kind of ideal method for preparing BiOI- protein composite nano plate CT images probe since invention the method equipment is simple, mild condition, low energy consumption, short preparation period, Yi Shixian.
Description
Technical field
The present invention relates to the preparation field of nanometer image probe, especially a kind of BiOI- protein composite nano plate CT shadow
As the preparation method of probe.
Background technique
The imaging of full name CT scan is imaged in CT, is exactly the usually said x-ray imaging of people.CT imaging with
Based on the three-dimensionalreconstruction of X-ray.Its basic principle is that different tissues have different X-ray absorption abilities, and its X-ray
Tissue penetration dosage also can be different.Through dosage and numerical digit geometric manipulations are utilized using measurement different parts tissue X-ray
Reconstruct tissue fault plane 3-dimensional image.But CT imaging technique is difficult to differentiate the little soft tissue of density difference, it is difficult to obtain
The clear image of the soft tissues such as lump, blood vessel.It is therefore desirable to which suitable medium (that is, CT image-forming contrast medium) carrys out enhanced CT imaging
Contrast.Need to meet as CT image-forming contrast medium: have powerful X ray attenuation ability, good biocompatibility with
And the conditions such as hypotoxicity.
Currently, almost all of internal CT image-forming contrast medium is all to close object containing iodine organification.Although they have good
Water solubility, good biocompatibility and the height endurability to human body, but the performance of these organic Cs T image-forming contrast medium sometimes because
Its unspecific organ distribution and it is different, can occur acute nephrotoxicity once in a while in shorter imaging time (i.e. about 1.5 hours).Cause
This, not yet meets the clinical demand with the CT image-forming contrast medium of longer blood circulatory half-life.
Use various nano particles as contrast agent, when imaging effect and blood circulation can be can increase by having been considered as one kind
Between to increase the solution of imaging time window.For example, it was discovered that the Bi of polymer overmold2S3Nanoparticle formulations have higher
X-ray absorption, longer circulation time and excellent imaging effect (Nat.Mater.2006,5,118.).However, in vivo
Hydrolyzed under acidic conditions Bi2S3It may cause hydrogen sulfide (H2S release), hydrogen sulfide be it is a kind of than hydrogen cyanide (HCN) to nerve and
The bigger gas of circulatory system toxicity.We begin look for the good bismuth compound of hydrolytic stability, and can be prepared into small
As graininess CT contrast agent, they have good biocompatibility and can be removed by kidney nano particle.
In conclusion disclosure sets forth the iodine oxidations that a kind of simple one-step method synthesis in water has biocompatibility
Bismuth-protein nano piece (BiOI), and study their applications as efficient CT contrast agent in experiment made on the living.
Summary of the invention
The present invention is mainly to provide a kind of simple preparation method of BiOI- protein nano piece, and this method has green side
Just, the features such as easily operated.The BiOI- protein nano piece being prepared has good biocompatibility and stability, and
And it can be applied to CT image-forming contrast medium.Gained BiOI- protein nano piece size is uniform, and preparation process is simple, number of devices
Amount is few, and short preparation period, low energy consumption, environmental-friendly, convenient for promoting.
To achieve the above object, the present invention adopts the following technical scheme that, a kind of BiOI- protein composite nano plate CT images
The preparation method of probe, comprising the following steps:
Mannitol is dissolved into protein aqueous solution by S1, and five nitric hydrate bismuths are then added, are stirred at room temperature and molten
Solution forms the mixed solution containing bismuth ion and protein；
S2 will be added dropwise in the mixed liquor of S1 containing iodine reagent, form Bi-I- protein mixed solution；
The mixed solution of S2 is stirred at room temperature 30~60min, then carries out centrifuge washing with deionized water by S3, from
Heart speed is 10000rpm, and 30 DEG C of vacuum drying 12h obtain BiOI- protein composite nano plate.
Technical solution as a further improvement of that present invention, the mannitol concentration in S1 is 2.5~20mmol/L, prevents bismuth
The hydrolysis of ion in aqueous solution.
Technical solution as a further improvement of that present invention, the concentration of the bismuth ion solution in S1 are 1~8mmol/L.
Technical solution as a further improvement of that present invention, the protein in S1 refer to bovine serum albumin(BSA), bovine hemoglobin
One of white, human serum albumins or human hemoglobin.
Technical solution as a further improvement of that present invention, the concentration of the protein aqueous solution in S1 are 1~10mg/mL.
Technical solution as a further improvement of that present invention, in S2 it is described containing iodine reagent refer to ammonium iodide, sodium iodide or
One of potassium iodide.
Technical solution as a further improvement of that present invention, the concentration containing iodine reagent in S2 are 15~650mmol/
L。
The medicine have the advantages that the present invention has directly synthesized BiOI- protein nano under room temperature environment in water phase
Piece, without high-temperature heating, required experimental facilities is simple, mild condition, low energy consumption, short preparation period, it is easy to accomplish, convenient for pushing away
Extensively；Meanwhile resulting BiOI- protein composite nano plate size is controllable, large specific surface area and size are uniform, reactivity site
Increase, good biocompatibility；In addition, resulting BiOI- protein composite nano plate can be dissolved in aqueous solution, and low toxicity, bio-compatible
Property it is good, can be applied to biology intracorporal CT imaging.
Detailed description of the invention
Fig. 1 is the transmission electron microscope image of the BiOI- protein nano piece obtained of the embodiment of the present invention 1.
Fig. 2 is X-ray powder diffraction (XRD) figure of the BiOI- protein nano piece obtained of the embodiment of the present invention 1.
Fig. 3 is the infrared spectrogram for the reactant protein used in the embodiment of the present invention 1.
Fig. 4 is the infrared spectrogram of the BiOI- protein nano piece obtained of the embodiment of the present invention 1.
Fig. 5 is the infrared spectrogram of comparative example 1 of the present invention BiOI nano material obtained.
Fig. 6 is the internal and external CT imaging of the BiOI- protein nano piece obtained of the embodiment of the present invention 1 and its strong
Degree-concentration relationship figure.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, right in the following with reference to the drawings and specific embodiments
The present invention is described in detail.
Embodiment 1:
S1: the mannitol of 0.5mmol being added into 25mL (5mg/mL) Bovine Serum Albumin in Aqueous Solution, and the ox blood is pure
At room temperature after stirring and dissolving, the five nitric hydrate bismuth particles of 0.2mmol, room is added in purity > 98%, the molecular weight 68000 of albumen
The lower stirring and dissolving 10min of temperature, forms the mixed solution containing bismuth ion and protein；
S2: 1.25mL (316mmol/L) IodineSodium Solution is added dropwise in the mixed liquor of bismuth ion and protein, institute
The bismuth ion of addition and the molar ratio of iodide ion are 1:2, stir 1h at room temperature, form BiOI- protein mixed liquor；
S3: by BiOI- protein mixed liquor with deionized water centrifuge washing 3 times, centrifugal speed 10000rpm, when centrifugation
Between be 10min, after centrifuge washing, BiOI- protein nano piece is made in 30 DEG C of vacuum drying treatment 12h.
The experimental results showed that under the conditions of nBi:nI=1:2 obtained BiOI- protein nano piece pattern be it is rectangular,
For length in 100nm or so, size is uniform, good dispersion.
Embodiment 2:
S1: the mannitol of 0.5mmol being added into 25mL (5mg/mL) Bovine Serum Albumin in Aqueous Solution, and the ox blood is pure
At room temperature after stirring and dissolving, the five nitric hydrate bismuth particles of 0.2mmol, room is added in purity > 98%, the molecular weight 68000 of albumen
The lower stirring and dissolving 10min of temperature, forms the mixed solution containing bismuth ion and protein；
S2: 1.25mL (158mmol/L) IodineSodium Solution is added dropwise in the mixed liquor of bismuth ion and protein, institute
The bismuth ion of addition and the molar ratio of iodide ion are 1:1, stir 1h at room temperature, form BiOI- protein mixed liquor；
S3: by BiOI- protein mixed liquor with deionized water centrifuge washing 3 times, centrifugal speed 10000rpm, when centrifugation
Between be 10min, after centrifuge washing, BiOI- protein nano piece is made in 30 DEG C of vacuum drying treatment 12h.
The experimental results showed that irregular in the resulting BiOI- protein nano piece pattern of nBi:nI=1:1, size is not yet
It is uniform, and it is few to be centrifuged product.
Embodiment 3:
S1: the mannitol of 0.5mmol being added into 25mL (5mg/mL) Bovine Serum Albumin in Aqueous Solution, and the ox blood is pure
At room temperature after stirring and dissolving, the five nitric hydrate bismuth particles of 0.2mmol, room is added in purity > 98%, the molecular weight 68000 of albumen
The lower stirring and dissolving 10min of temperature, forms the mixed solution containing bismuth ion and protein；
S2: 1.25mL (474mmol/L) IodineSodium Solution is added dropwise in the mixed liquor of bismuth ion and protein, institute
The bismuth ion of addition and the molar ratio of iodide ion are 1:3, stir 1h at room temperature, form BiOI- protein mixed liquor；
S3: by BiOI- protein mixed liquor with deionized water centrifuge washing 3 times, centrifugal speed 10000rpm, when centrifugation
Between be 10min, after centrifuge washing, BiOI- protein nano piece is made in 30 DEG C of vacuum drying treatment 12h.
The experimental results showed that resulting BiOI- protein nano piece pattern is 100nm left under the conditions of nBi:nI=1:3
Right is rectangular, and size is uniform, but bad dispersibility, is easy aggregation.
Embodiment 5:
S1: the mannitol of 0.5mmol, the human seralbumin are added into 25mL (5mg/mL) human serum albumins aqueous solution
At room temperature after stirring and dissolving, the five nitric hydrate bismuth particles of 0.2mmol, room is added in purity > 98%, the molecular weight 68000 of albumen
The lower stirring and dissolving 10min of temperature, forms the mixed solution containing bismuth ion and protein；
S2: 1.25mL (316mmol/L) IodineSodium Solution is added dropwise in the mixed liquor of bismuth ion and protein, institute
The bismuth ion of addition and the molar ratio of iodide ion are 1:2, stir 1h at room temperature, form BiOI- protein mixed liquor；
S3: by BiOI- protein mixed liquor with deionized water centrifuge washing 3 times, centrifugal speed 10000rpm, when centrifugation
Between be 10min, after centrifuge washing, BiOI- protein nano piece is made in 30 DEG C of vacuum drying treatment 12h.
The experimental results showed that resulting BiOI nano material size is uniform under the conditions of existing for the human serum albumins, point
It is good to dissipate property.
Embodiment 6:
S1: the mannitol of 0.5mmol being added into 25mL (5mg/mL) Bovine Serum Albumin in Aqueous Solution, and the ox blood is pure
At room temperature after stirring and dissolving, the five nitric hydrate bismuth particles of 0.2mmol, room is added in purity > 98%, the molecular weight 68000 of albumen
The lower stirring and dissolving 10min of temperature, forms the mixed solution containing bismuth ion and protein；
S2: 1.25mL (316mmol/L, nBi:nI=1:2) liquor kalii iodide is added dropwise to bismuth ion and protein
Mixed liquor in, the molar ratio of the bismuth ion and iodide ion that are added is 1:3, stirs 1h at room temperature, and it is mixed to form BiOI- protein
Close liquid；
S3: by BiOI- protein mixed liquor with deionized water centrifuge washing 3 times, centrifugal speed 10000rpm, when centrifugation
Between be 10min, after centrifuge washing, BiOI- protein nano piece is made in 30 DEG C of vacuum drying treatment 12h.
The experimental results showed that use potassium iodide as the resulting BiOI- protein nano piece of under conditions of containing iodine reagent
Size is uniform, good dispersion.
Comparative example 1:
S1: the mannitol that 0.5mmol is added into 25mL deionized water is added 0.2mmol's at room temperature after stirring and dissolving
Five nitric hydrate bismuth particles, stirring and dissolving 10min, forms bismuth ion solution at room temperature；
S2: 1.25mL (316mmol/L) IodineSodium Solution is added dropwise in bismuth ion solution, the bismuth ion being added
Molar ratio with iodide ion is 1:2, stirs 1h at room temperature, forms BiOI dirty solution；
S3: by BiOI dirty solution with deionized water centrifuge washing 3 times, centrifugal speed 10000rpm, centrifugation time is
10min, after centrifuge washing, BiOI nano material is made in 30 DEG C of vacuum drying treatment 12h.
The experimental results showed that resulting BiOI appearance of nano material is irregular under the conditions of no bovine serum albumin, size is not
It is uniform, bad dispersibility.
Comparative example 2:
S1: the five nitric hydrate bismuth particles of 0.2mmol, institute are added into 25mL (5mg/mL) Bovine Serum Albumin in Aqueous Solution
Purity > 98%, the molecular weight 68000 of bovine serum albumin(BSA) are stated, at room temperature stirring and dissolving 10min, forms bismuth ion containing z and albumen
The mixed solution of matter；
S2: 1.25mL (316mmol/L, nBi:nI=1:2) IodineSodium Solution is added dropwise to bismuth ion and protein
Mixed liquor in, the molar ratio of the bismuth ion and iodide ion that are added is 1:2, stirs 1h at room temperature, and it is mixed to form BiOI- protein
Close liquid；
The experimental results showed that bismuth ion is easily hydrolyzed into the solid insoluble of white in the case where no mannitol, cause
Reaction cannot normally continue.
Above embodiments are merely to illustrate the present invention and not limit the technical scheme described by the invention, to this specification
Understanding should based on person of ordinary skill in the field, although this specification referring to the above embodiments to the present invention
Detailed description is had been carried out, still, those skilled in the art should understand that, person of ordinary skill in the field is still
Can so modify or equivalently replace the present invention, and all do not depart from the spirit and scope of the present invention technical solution and
It is improved, and should all be covered in scope of the presently claimed invention.
Claims (7)
1. a kind of preparation method of BiOI- protein composite nano plate CT images probe, which comprises the following steps:
Mannitol is dissolved into protein aqueous solution by S1, and five nitric hydrate bismuths are then added, is stirred at room temperature and dissolves,
Form the mixed solution containing bismuth ion and protein；
S2 will be added dropwise in the mixed liquor of S1 containing iodine reagent, form Bi-I- protein mixed solution；
30~60min is stirred at room temperature in the mixed solution of S2 by S3, then carries out centrifuge washing, centrifugation speed with deionized water
Degree is 10000rpm, and 30 DEG C of vacuum drying 12h obtain BiOI- protein composite nano plate.
2. the preparation method of BiOI- protein composite nano plate CT images probe as described in claim 1, it is characterised in that:
Mannitol concentration in S1 is 2.5~20mmol/L, prevents the hydrolysis of bismuth ion in aqueous solution.
3. the preparation method of BiOI- protein composite nano plate CT images probe as described in claim 1, it is characterised in that:
The concentration of bismuth ion solution in S1 is 1~8mmol/L.
4. the preparation method of BiOI- protein composite nano plate CT images probe as described in claim 1, it is characterised in that:
Protein in S1 refers to one of bovine serum albumin(BSA), bovine hemoglobin, human serum albumins or human hemoglobin.
5. the preparation method of BiOI- protein composite nano plate CT images probe as described in claim 1 or 4, feature exist
In: the concentration of the protein aqueous solution in S1 is 1~10mg/mL.
6. the preparation method of BiOI- protein composite nano plate CT images probe as described in claim 1, it is characterised in that:
The iodine reagent that contains in S2 refers to one of ammonium iodide, sodium iodide or potassium iodide.
7. the preparation method of BiOI- protein composite nano plate CT images probe as described in claim 1 or 6, feature exist
In: the concentration containing iodine reagent in S2 is 15~650mmol/L.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104876266A (en) * | 2015-04-21 | 2015-09-02 | 南京邮电大学 | Aqueous-phase preparation method of bismuth sulfide/protein composite nanospheres |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104876266A (en) * | 2015-04-21 | 2015-09-02 | 南京邮电大学 | Aqueous-phase preparation method of bismuth sulfide/protein composite nanospheres |
Non-Patent Citations (1)
| Title |
|---|
| MURTHI S. K等: "Synthesis, Characterization, and X ray Attenuation Properties of Ultrasmall BiOI Nanoparticles: Toward Renal Clearable Particulate CT Contrast Agents", 《INORGANIC CHEMISTRY》 * |
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