CN110476812B

CN110476812B - Method for rapid breeding of Maranta americana

Info

Publication number: CN110476812B
Application number: CN201910770752.9A
Authority: CN
Inventors: 何文杰; 陈慧; 余结梅; 郑慈真; 江碧玉; 马文卿
Original assignee: Guangzhou Minghui Landscape Technology Development Co ltd
Current assignee: Guangzhou Minghui Landscape Technology Development Co ltd
Priority date: 2019-08-20
Filing date: 2019-08-20
Publication date: 2022-06-07
Anticipated expiration: 2039-08-20
Also published as: CN110476812A

Abstract

The invention relates to the field of plant planting, and provides a method for rapid breeding of Maranta arundinacea, which is used for improving the breeding efficiency of the Maranta arundinacea. The invention provides a method for rapid breeding of Maranta americana, which comprises the following steps: taking the female parent of the beautiful arrowroot, taking lateral buds, stripping off part of leaf sheaths, cleaning, soaking in alcohol, and soaking in disinfectant for disinfection; stripping off part of leaf sheaths, soaking the leaf sheaths in disinfectant again, taking out the leaf sheaths after a period of time, cleaning the leaf sheaths, and stripping off the residual leaf sheaths to obtain explants; performing adventitious bud induction culture on the explant until adventitious bud plantlets are obtained; taking adventitious bud plantlets, reserving stem sections, carrying out adventitious bud multiplication culture on the stem sections, and then carrying out rooting induction culture until complete plant tissue culture plantlets with main roots are obtained; and cleaning the tissue culture seedlings, and transplanting the tissue culture seedlings into a matrix to obtain a large number of beautiful arrowroot seedlings. The survival rate of the seedlings in the culture process is high, more beautiful arrowroot plants can be obtained in the same culture time, and the propagation efficiency is greatly improved.

Description

Method for rapid breeding of arrowroot

Technical Field

The invention relates to the technical field, in particular to a method for rapid breeding of Maranta arundinacea.

Background

Beautiful arrowroot (academic name:Calatheaveitchiana) Is perennial herb foliage plant of the genus showy of the family arrowroot. Original productions of ecuador, peru, etc. The foliage plants are good indoor foliage plants which are fond of yin and are in a warm, humid and semi-yin growing environment, are afraid of dryness and are not exposed to strong light, grow at a proper temperature of 22-28 ℃. The utility model is used for arranging places such as bedrooms, living rooms and offices. Although the arrowroot is produced in large scale in China, the great demand of the market on the beautiful arrowroot cannot be met. The main reason is that the beautiful arrowroot is difficult to seed, the common growers can only breed the beautiful arrowroot by dividing the plant, the growth period of the beautiful arrowroot is long, and a large amount of finished products are difficult to breed quickly.

The tissue culture technique is a technique in which living isolated organs (such as roots, stems, leaves, stem segments, protoplasts), tissues or cells are placed in a culture medium under artificially created aseptic conditions and placed in an appropriate environment for continuous culture to obtain cells, tissues or individuals.

How to rapidly propagate a large amount of beautiful arrowroot seedlings by utilizing a tissue culture technology to solve the market demand is a technical problem to be solved urgently.

Disclosure of Invention

The invention solves the technical problem of providing a method for rapid breeding of beautiful arrowroot.

In order to solve the technical problems, the technical scheme provided by the invention is as follows:

a method for rapid propagation of Maranta arundinacea, comprising: s11, taking the female parent of the beautiful arrowroot, sterilizing, performing tube culture, taking lateral buds, removing part of leaf sheaths, cleaning, soaking in alcohol, soaking in a disinfectant for sterilization, taking out for cleaning after a period of time, removing part of leaf sheaths, soaking in the disinfectant again, taking out for cleaning after a period of time, removing the rest leaf sheaths, and obtaining an explant; s12, carrying out adventitious bud induction culture on the explant until adventitious bud plantlets are obtained; s13, dividing adventitious bud seedlings into single plants, cutting off leaves, reserving stem sections, and carrying out adventitious bud multiplication culture on the stem sections until 2-3 adventitious bud seedlings grow out of the stem sections again; s14, repeating S13 for a certain number of times until a certain number of adventitious bud seedlings are obtained; s15, cutting the obtained adventitious bud plantlet into single plantlets, and carrying out rooting induction culture until a complete plant tissue culture seedling with a main root is obtained; s16, the tissue culture seedlings are washed and then transplanted into a matrix, and a large number of beautiful arrowroot seedlings are obtained.

The female parent of the beautiful arrowroot is disinfected for a plurality of times and then is sequentially subjected to induction, proliferation and rooting culture, so that a large number of plants of the beautiful arrowroot can be obtained, and the market demand is met.

The survival rate of the seedlings in the culture process is high, more beautiful arrowroot plants can be obtained in the same culture time, and the propagation efficiency is greatly improved.

Preferably, in the step S11, the female parent of the arrowroot is taken and sterilized by a chlorothalonil solution, wherein the chlorothalonil solution is chlorothalonil: a diluent of solvent =1: 1000-3000; the tube culture is to transplant the female parent into a clean greenhouse for culture, the environment temperature is controlled to be 18-30 ℃, the humidity is 80-95%, and the illumination intensity is not more than 10000 lux; the length of the lateral buds is 2-5 cm, partial leaf sheaths are stripped, the innermost 3-5 layers of clean leaf sheaths are reserved, the leaf sheaths are soaked in clear water for 1-10 min, the liquid is washed clean, then soaked in 50-80% alcohol for 15-60 s, and then soaked in 0.2-0.8% sodium hypochlorite solution for disinfection for 10-12 min, and the mixture is continuously stirred in the disinfection process; taking out the disinfected lateral buds, washing with water, removing 1-2 outermost leaf sheaths, repairing the wound to be flat, and then placing in a 0.2-0.8% sodium hypochlorite solution for disinfection for 10-15 min; the sodium hypochlorite solution contains 1-2 drops of Tween 80; taking out the lateral bud, washing with water, stripping off the remaining leaf sheath, and removing the injured tissue after sterilization to obtain the explant. The lateral buds are disinfected for a plurality of times, and the leaf sheaths are gradually stripped in the disinfection process, so that the tissue quantity is ensured to be sufficient while the disinfection is carried out as far as possible.

Preferably, in the step S12, when the adventitious bud induction culture is performed on the explant, the environment temperature is controlled to be 22-24 ℃, the illumination time is 10h/d, the illumination intensity is 2000-3000 lux, and the culture is performed for 60-80 d. Inducing the plant to become adventitious bud plantlet and raising the propagation efficiency.

Preferably, in the step S13, the stem segments are kept for 0.5-2 cm; when the adventitious bud propagation culture is carried out, the environmental temperature is controlled to be 22-24 ℃, the illumination time is 10h/d, the illumination intensity is 2000-3000 lux, and the culture is carried out for 60-80 d; in the step S14, the step S13 is repeated 15 to 20 times. The single multiplication coefficient is 2.0-3.0, and a large number of plants can be obtained 15-20 times.

Preferably, in the step S15, in the rooting induction culture process, the environmental temperature is controlled to be 24-28 ℃, the illumination time is 10h/d, the illumination intensity is 2000-3000 lux, and the culture is carried out for 30-40 d; and obtaining the tissue culture seedling of the complete plant with 2-3 main roots. The adventitious bud plantlet is induced to root, the rooting rate can reach 95 percent, and the propagation efficiency is further improved.

Preferably, in the step S16, the tissue culture seedling is washed clean with water, transferred to a substrate, and irrigated with chlorothalonil solution, with the ambient temperature controlled at 26-30 ℃ and the humidity controlled at 80-95%; the matrix is a mixture of peat and coconut coir, wherein the peat: the coconut coir = 1-6: 1, and the thickness of the peat is 5-25; culturing in the matrix until new leaves grow, and spraying fertilizer water. The culture on peat and coconut husk substrate can further ensure very high survival rate, and the survival rate can reach 90%.

Preferably, the culture medium selected in the adventitious bud induction culture in the step S12, the adventitious bud proliferation culture in the step S13, and the rooting culture in the step S15 includes: NH (NH)₄NO₃ 825~1650mg/L、KNO₃ 950~1900mg/L、MgSO₄·7H₂O 185~370mg/L、KH₂PO₄ 120~170mg/L、CaCl₂·2H₂O 220~440mg/L、KI 0.42~0.83mg/L、H₃BO₃ 3.1~6.2mg/L、MnSO₄·4H₂O 11.1~22 .3mg/L、ZnSO₄·7H₂O 4.3~8.6mg/L、Na₂MoO₄·2H₂O 0.1~0.25mg/L、CuSO₄·5H₂O 0.01~0.025mg/L CoCl₂·6H₂O 0.01~0.025mg/L、FeSO₄·7H₂O 13.5~27.8mg/L、Na₂16.3-37.3 mg/L EDTA, 0.05-0.1 mg/L thiamine hydrochloride, 0.2-0.5 mg/L nicotinic acid, 0.3-0.5 mg/L pyridoxine hydrochloride, 1-2 mg/L glycine, and 50-100 mg/L inositol. The culture medium ensures that the tissue has access to sufficient nutrients.

Preferably, in the step S12, an induction medium is selected for adventitious bud induction culture of the explant, and the induction medium further includes 2.0-4.0 mg/L of 6-benzylaminopurine, 0.1-0.2 mg/L of naphthylacetic acid, 6.2g/L of carrageenan, and 30g/L of white sugar. A certain amount of 6-benzylaminopurine, naphthylacetic acid and carrageenan are added into an induction culture medium, so that the tissue conversion into adventitious bud plantlets can be effectively promoted.

Preferably, in the step S13, a multiplication medium is selected for multiplication culture of adventitious buds, and the multiplication medium further includes 0.5-1.0 mg/L of 6-benzylaminopurine, 0.05-0.1 mg/L of naphthylacetic acid, 6.2g/L of carrageenan, and 30g/L of white sugar. The content of 6-benzylaminopurine, naphthylacetic acid and carrageenan is adjusted to promote the proliferation of adventitious bud plantlets.

Preferably, in the step S15, a rooting medium is selected during the rooting induction culture process, and the rooting medium further includes 3g/L of activated carbon, 0.4-0.5 mg/L of naphthylacetic acid, 7g/L of carrageenan, and 20g/L of white sugar. The activated carbon is added into the culture medium, so that the rooting efficiency of the plants can be obviously improved, and the survival rate is further improved.

Compared with the prior art, the invention has the beneficial effects that: the survival rate of the seedlings in the culture process is high, more beautiful arrowroot plants can be obtained in the same culture time, and the propagation efficiency is greatly improved.

The multiplication coefficient of the method is 2.0-3.0 in the multiplication culture step of the adventitious bud, the total subculture multiplication is 15-20 times, and compared with a general plant division propagation method, the multiplication coefficient is greatly improved. After the environmental steps of adventitious bud induction culture, adventitious bud propagation culture and rooting culture, the strong tissue culture seedling can be stably propagated for a long time, and the survival rate of the tissue culture seedling after field planting can reach 90 percent

Detailed Description

The following examples are further illustrative of the present invention and are not intended to be limiting thereof.

Example 1

A method for rapid propagation of Maranta arundinacea, comprising: s11, taking the female parent of the beautiful arrowroot, sterilizing, performing tube culture, taking lateral buds, removing part of leaf sheaths, cleaning, soaking in alcohol, soaking in a disinfectant for sterilization, taking out for cleaning after a period of time, removing part of leaf sheaths, soaking in the disinfectant again, taking out for cleaning after a period of time, removing the rest leaf sheaths, and obtaining an explant; s12, carrying out adventitious bud induction culture on the explant until adventitious bud plantlets are obtained; s13, dividing adventitious bud seedlings into single plants, cutting off leaves, reserving stem sections, and carrying out adventitious bud multiplication culture on the stem sections until 2-3 adventitious bud seedlings grow out of the stem sections again; s14, repeating S13 for a certain number of times until a certain number of adventitious bud seedlings are obtained; s15, cutting the obtained adventitious bud plantlet into single plantlets, and carrying out rooting induction culture until a complete plant tissue culture seedling with a main root is obtained; s16, the tissue culture seedlings are washed and then transplanted into a matrix, and a large number of beautiful arrowroot seedlings are obtained. In the step of S11, rhizoma Marantae Arundinaceae is takenAnd (3) irrigating and disinfecting the female parent by using a chlorothalonil solution, wherein the chlorothalonil solution is chlorothalonil: solvent =1:2000 dilution; the tube culture is to transplant the female parent into a clean greenhouse for culture, the environment temperature is controlled to be 18-30 ℃, the humidity is 80-95%, and the illumination intensity is not more than 10000 lux; the length of the lateral bud is 2-5 cm, partial leaf sheaths are stripped off on an ultra-clean workbench, 2-3 innermost layers of clean leaf sheaths are reserved, a scalpel is used for cutting a cut to be flat, detergent is added into clean water for soaking for 5min, after the cut is washed clean by the clean water, the cut is soaked in 75% alcohol for 30s and then soaked in 0.6% sodium hypochlorite solution for disinfection for 10-12 min, and stirring is carried out continuously in the disinfection process; taking out the sterilized lateral buds, cleaning the lateral buds with water, removing 1-2 outermost leaf sheaths, repairing the wound to be flat with a scalpel, and then putting the wound into a 0.6% sodium hypochlorite solution for sterilization for 10-15 min; the sodium hypochlorite solution contains 1-2 drops of Tween 80; taking out the lateral bud, washing with water, stripping off the remaining leaf sheath, and removing the injured tissue after disinfection to obtain the explant. And in the step S12, when the adventitious bud induction culture is carried out on the explant, the environment temperature is controlled to be 22-24 ℃, the illumination time is 10h/d, the illumination intensity is 2000-3000 lux, and the culture is carried out for 60-80 d. In the step S13, reserving 1cm of stem segments; when the adventitious bud propagation culture is carried out, the environmental temperature is controlled to be 22-24 ℃, the illumination time is 10h/d, the illumination intensity is 2000-3000 lux, and the culture is carried out for 60-80 d; in the step S14, the step S13 is repeated 15-20 times. In the step S15, in the rooting induction culture process, the environmental temperature is controlled to be 24-28 ℃, the illumination time is 10h/d, the illumination intensity is 2000-3000 lux, and the culture is carried out for 30-40 d; and obtaining the tissue culture seedling of the complete plant with 2-3 main roots. In the step S16, the tissue culture seedlings are washed clean by water, transferred to a substrate and irrigated by chlorothalonil solution, and the environmental temperature is controlled to be 26-30 ℃ and the humidity is controlled to be 80-95%; the matrix is a mixture of peat and coconut coir, wherein the peat: the coconut coir =4:1, and the thickness of the peat is 5-25; culturing in the matrix until new leaves grow, and spraying fertilizer water. In the step S12, an induction medium is selected for adventitious bud induction culture of the explant, wherein the induction medium includes: NH (NH)₄NO₃ 1650mg/L、KNO₃1900mg/L、MgSO₄·7H₂O 370mg/L、KH₂PO₄ 170mg/L、CaCl₂·2H₂O 440mg/L、KI 0.83mg/L、H₃BO₃ 6.2mg/L、MnSO₄·4H₂O 22 .3mg/L、ZnSO₄·7H₂O 8.6mg/L、Na₂MoO₄·2H₂O 0.25mg/L、CuSO₄·5H₂O 0.025mg/L CoCl₂·6H₂O 0.025mg/L、FeSO₄·7H₂O 27.8mg/L、Na₂37.3mg/L of EDTA, 0.1mg/L of thiamine hydrochloride, 0.5mg/L of nicotinic acid, 0.5mg/L of pyridoxine hydrochloride, 1-2 mg/L of glycine, 100mg/L of inositol, 2.0-4.0 mg/L of 6-benzylaminopurine, 0.1-0.2 mg/L of naphthylacetic acid, 6.2g/L of carrageenan and 30g/L of white sugar. In the step S13, a multiplication medium is selected when performing multiplication culture of adventitious buds, and the multiplication medium includes: NH (NH)₄NO₃ 1650mg/L、KNO₃1900mg/L、MgSO₄·7H₂O 370mg/L、KH₂PO₄170mg/L、CaCl₂·2H₂O 440mg/L、KI 0.83mg/L、H₃BO₃ 6.2mg/L、MnSO₄·4H₂O 22 .3mg/L、ZnSO₄·7H₂O 8.6mg/L、Na₂MoO₄·2H₂O 0.25mg/L、CuSO₄·5H₂O 0.025mg/L CoCl₂·6H₂O 0.025mg/L、FeSO₄·7H₂O 27.8mg/L、Na₂37.3mg/L of EDTA, 0.1mg/L of thiamine hydrochloride, 0.5mg/L of nicotinic acid, 0.5mg/L of pyridoxine hydrochloride, 1-2 mg/L of glycine, 100mg/L of inositol, 0.5-1.0 mg/L of 6-benzylaminopurine, 0.05-0.1 mg/L of naphthylacetic acid, 6.2g/L of carrageenan and 30g/L of white sugar. In the step S15, a rooting medium is selected during the rooting induction culture process, and the rooting medium includes: NH (NH)₄NO₃825mg/L、KNO₃950mg/L、MgSO₄·7H₂O 185mg/L、KH₂PO₄ 170mg/L、CaCl₂·2H₂O 220mg/L、KI 0.83mg/L、H₃BO₃ 6.2mg/L、MnSO₄·4H₂O 22 .3mg/L、ZnSO₄·7H₂O 8.6mg/L、Na₂MoO₄·2H₂O 0.25mg/L、CuSO₄·5H₂O 0.025mg/L CoCl₂·6H₂O 0.025mg/L、FeSO₄·7H₂O 27.8mg/L、Na₂37.3mg/L of EDTA, 0.1mg/L of thiamine hydrochloride, 0.5mg/L of nicotinic acid, 0.5mg/L of pyridoxine hydrochloride, 1-2 mg/L of glycine, 100mg/L of inositol, 3g/L of activated carbon, 0.4-0.5 mg/L of naphthylacetic acid, 7g/L of carrageenan and 20g/L of white sugar.

The female parent of the beautiful arrowroot is disinfected for a plurality of times and then is sequentially subjected to induction, proliferation and rooting culture, so that a large number of plants of the beautiful arrowroot can be obtained, and the market demand is met. The survival rate of the seedlings in the culture process is high, more beautiful arrowroot plants can be obtained in the same culture time, and the propagation efficiency is greatly improved. The lateral buds are disinfected for a plurality of times, and the leaf sheaths are gradually stripped in the disinfection process, so that the tissue quantity is ensured to be sufficient while the disinfection is carried out as far as possible. Inducing the plant to become adventitious bud plantlet and raising the propagation efficiency. The single multiplication coefficient is 2.0-3.0, and a large number of plants can be obtained 15-20 times. The adventitious bud plantlet is induced to root, the rooting rate can reach 95 percent, and the propagation efficiency is further improved. The culture on peat and coconut husk substrate can further ensure very high survival rate, and the survival rate can reach 90%. The culture medium ensures that the tissue has access to sufficient nutrients. A certain amount of 6-benzylaminopurine, naphthylacetic acid and carrageenan are added into an induction culture medium, so that the tissue conversion into adventitious bud plantlets can be effectively promoted. The content of 6-benzylaminopurine, naphthylacetic acid and carrageenan is adjusted to promote the proliferation of adventitious bud plantlet. The activated carbon is added into the culture medium, so that the rooting efficiency of the plants can be obviously improved, and the survival rate is further improved.

Example 2

Example 2 is the same as example 1 except that, in the step S11, female arrowroot is taken and sterilized by drenching with a chlorothalonil solution, wherein the chlorothalonil solution is chlorothalonil: solvent =1:2000 dilution; the tube culture is to transplant the female parent into a clean greenhouse for culture, the environmental temperature is controlled to be 15-17 ℃, the humidity is 80-95%, and the illumination intensity is not more than 10000 lux; the length of the lateral buds is 2-5 cm, partial leaf sheaths are stripped on an ultra-clean workbench, 2-3 innermost clean leaf sheaths are reserved, a scalpel is used for cutting the cut to be flat, detergent is added into clean water for soaking for 5min, after the cut is washed clean, the cut is soaked in 75% alcohol for 30s and then soaked in 0.6% sodium hypochlorite solution for disinfection for 10-12 min, and stirring is carried out continuously in the disinfection process; taking out the sterilized lateral buds, cleaning the lateral buds with water, removing 1-2 outermost leaf sheaths, repairing the wound to be flat with a scalpel, and then putting the wound into a 0.6% sodium hypochlorite solution for sterilization for 10-15 min; the sodium hypochlorite solution contains 1-2 drops of Tween 80; taking out the lateral bud, washing with water, stripping off the remaining leaf sheath, and removing the injured tissue after sterilization to obtain the explant.

Example 3

The difference between the embodiment 3 and the embodiment 1 is that in the step S12, when the adventitious bud induction culture is performed on the explant, the environment temperature is controlled to be 25-26 ℃, the illumination time is 10h/d, the illumination intensity is 2000-3000 lux, and the culture is performed for 60-80 d.

Example 4

Example 4 differs from example 1 in that in the step of S13, 1cm of stem segment was retained; when the adventitious bud propagation culture is carried out, the environmental temperature is controlled to be 25-26 ℃, the illumination time is 10h/d, the illumination intensity is 2000-3000 lux, and the culture is carried out for 60-80 d; in the step S14, the step S13 is repeated 15-20 times.

Example 5

The embodiment 5 is different from the embodiment 1 in that in the step S15, in the rooting induction culture process, the environmental temperature is controlled to be 29 to 30 ℃, the illumination time is 10h/d, the illumination intensity is 2000 to 3000lux, and the culture is carried out for 30 to 40 d.

Example 6

The embodiment 6 is different from the embodiment 1 in that in the step S16, the tissue culture seedling is washed clean with water, transferred to a substrate, and irrigated with a chlorothalonil solution, the environmental temperature is controlled to be 22-24 ℃, and the humidity is controlled to be 80-95%; the matrix is a mixture of peat and coconut coir, wherein the peat: the coconut coir =4:1, and the thickness of the peat is 5-25; culturing in the matrix until new leaves grow, and spraying fertilizer water.

Example 7

The embodiment 7 is the same as the embodiment 1 except that in the step S16, the tissue culture seedling is washed clean with water, transferred to a substrate, and irrigated with a chlorothalonil solution, the environmental temperature is controlled to be 26-30 ℃, and the humidity is controlled to be 80-95%; the medium is peat, and the fineness of the peat is 5-25; culturing in the matrix until new leaves grow, and spraying fertilizer water.

Example 8

Example 8 is different from example 1 in that the culture medium selected for adventitious bud induction culture in the step S12, adventitious bud proliferation culture in the step S13, and rooting culture in the step S15 includes: NH (NH)₄NO₃ 825~1650mg/L、KNO₃950~1900mg/L、MgSO₄·7H₂O 185~370mg/L、KH₂PO₄ 120~170mg/L、CaCl₂·2H₂O 220~440mg/L、KI 0.42~0.83mg/L、H₃BO₃ 3.1~6.2mg/L、MnSO₄·4H₂O 11.1~22 .3mg/L、ZnSO₄·7H₂O 4.3~8.6mg/L、Na₂MoO₄·2H₂O 0.1~0.25mg/L、CuSO₄·5H₂O 0.01~0.025mg/L CoCl₂·6H₂O 0.01~0.025mg/L、FeSO₄·7H₂O 13.5~27.8mg/L、Na₂16.3-37.3 mg/L EDTA, 0.05-0.1 mg/L thiamine hydrochloride, 0.2-0.5 mg/L nicotinic acid, 0.3-0.5 mg/L pyridoxine hydrochloride, 1-2 mg/L glycine, and 50-100 mg/L inositol.

Comparative example 1

Comparative example 1 the difference from example 1 is that, in the step S11, female arrowroot is taken and sterilized by drenching with a chlorothalonil solution, wherein the chlorothalonil solution is chlorothalonil: solvent =1:2000 dilution; the tube culture is to transplant the female parent into a clean greenhouse for culture, the environmental temperature is controlled to be 18-30 ℃, the humidity is 80-95%, and the illumination intensity is not more than 10000 lux; the length of the lateral bud is 2-5 cm, all leaf sheaths are stripped on an ultra-clean workbench, a cut is cut flatly by using a scalpel, detergent is added into clear water to soak for 5min, after the clean water is washed, the cut is soaked in 75% alcohol for 30s and then soaked in 0.6% sodium hypochlorite solution to be disinfected for 10-12 min, and the stirring is continuously carried out in the disinfection process; taking out the lateral bud, washing with water, and removing the injured tissue after disinfection to obtain the explant.

Examples of the experiments

And counting the final yield of the beautiful arrowroot in the examples 1-8 and the comparative example 1.

From example 1, it can be seen that the mother plant pretreatment mode of the present application, the environmental conditions of induction, proliferation and rooting, the culture medium and the substrate for the growth of the tissue culture seedling can significantly improve the yield of the arrowroot.

The temperature of mother tube culture in example 2 was different from that in example 1, the temperature of adventitious bud induction in example 3 was different from that in example 1, the temperature of adventitious bud proliferation in example 4 was different from that in example 1, the temperature of rooting induction in example 5 was different from that in example 1, the temperature of tissue culture seedling growth in example 6 was different from that in example 1, the substrate of tissue culture seedling growth in example 7 was different from that in example 1, the medium of tissue culture seedling growth in example 8 was different from that in example 1, and the yield of the tissue culture seedling was not sterilized many times in comparative example 1 and was lower than that in example 1, indicating that various parameters in the present application have important effects on the improvement of the yield of arrowroot.

The above detailed description is specific to possible embodiments of the present invention, and the above embodiments are not intended to limit the scope of the present invention, and all equivalent implementations or modifications that do not depart from the scope of the present invention should be included in the present claims.

Claims

1. A method for rapid propagation of Maranta arundinacea, which is characterized by comprising the following steps:

s11, taking the female parent of the beautiful arrowroot, sterilizing, performing tube culture, taking lateral buds, removing part of leaf sheaths, cleaning, soaking in alcohol, soaking in a disinfectant for sterilization, taking out for cleaning after a period of time, removing part of leaf sheaths, soaking in the disinfectant again, taking out for cleaning after a period of time, removing the rest leaf sheaths, and obtaining an explant; in the step of S11, taking the female parent of the beautiful arrowroot, and irrigating and disinfecting the female parent with chlorothalonil solution, wherein the chlorothalonil solution is chlorothalonil: the solvent is a diluent of 1: 1000-3000; the tube culture is to transplant the female parent into a clean greenhouse for culture, the environment temperature is controlled to be 18-30 ℃, the humidity is 80-95%, and the illumination intensity is not more than 10000 lux; the length of the lateral bud is 2-5 cm, part of leaf sheaths are stripped, the innermost 3-5 layers of clean leaf sheaths are reserved, the clean leaf sheaths are soaked for 1-10 min by adding detergent into clean water, the leaf sheaths are soaked for 15-60 s by 50-80% alcohol after being washed clean by the clean water, the leaf sheaths are soaked in 0.2-0.8% sodium hypochlorite solution for disinfection for 10-12 min, and stirring is carried out continuously in the disinfection process; taking out the disinfected lateral buds, washing with water, removing 1-2 outermost leaf sheaths, repairing the wound to be flat, and then placing in a 0.2-0.8% sodium hypochlorite solution for disinfection for 10-15 min; the sodium hypochlorite solution contains 1-2 drops of Tween 80; taking out the lateral bud, washing with water, stripping off the remaining leaf sheath, and removing the injured tissue after disinfection to obtain an explant;

s12, performing adventitious bud induction culture on the explant until adventitious bud plantlets are obtained;

s13, dividing adventitious bud seedlings into single plants, cutting off leaves, reserving stem sections, and carrying out adventitious bud multiplication culture on the stem sections until 2-3 adventitious bud seedlings grow out of the stem sections again;

s14, repeating S13 for a certain number of times until a certain number of adventitious bud seedlings are obtained;

s15, cutting the obtained adventitious bud plantlets into single plantlets, and performing rooting induction culture until complete plant tissue culture plantlets with main roots are obtained;

s16, the tissue culture seedlings are washed and then transplanted into a matrix, and a large number of beautiful arrowroot seedlings are obtained.

2. The method for rapid propagation of arrowroot as claimed in claim 1, wherein in the step S12, when the adventitious bud induction culture is performed on the explant, the environmental temperature is controlled to be 22-24 ℃, the illumination time is 10h/d, the illumination intensity is controlled to be 2000-3000 lux, and the culture is performed for 60-80 d.

3. The method for rapid propagation of Maranta americana according to claim 1, wherein in the step of S13, 0.5-2 cm of stem is reserved; when the adventitious bud propagation culture is carried out, the environmental temperature is controlled to be 22-24 ℃, the illumination time is 10h/d, the illumination intensity is 2000-3000 lux, and the culture is carried out for 60-80 d; in the step S14, the step S13 is repeated 15 to 20 times.

4. The method for rapid propagation of arrowroot according to claim 1, wherein in the step S15, the environmental temperature is controlled to be 24-28 ℃, the illumination time is 10h/d, the illumination intensity is controlled to be 2000-3000 lux, and the rooting induction culture process is carried out for 30-40 d; and obtaining the tissue culture seedling of the complete plant with 2-3 main roots.

5. The method for rapid propagation of arrowroot as claimed in claim 1, wherein in the step S16, the tissue culture seedling is washed clean with water, transferred to a substrate, irrigated with chlorothalonil solution, the environmental temperature is controlled at 26-30 ℃ and the humidity is controlled at 80-95%; the matrix is a mixture of peat and coconut coir, wherein the peat: the coconut coir = 1-6: 1, and the thickness of the peat is 5-25; culturing in the matrix until new leaves grow, and spraying fertilizer water.

6. The method of claim 1, wherein the culture medium selected for adventitious bud induction culture in the step S12, adventitious bud proliferation culture in the step S13, and rooting culture in the step S15 comprises: NH (NH)₄NO₃ 825~1650mg/L、KNO₃950~1900mg/L、MgSO₄·7H₂O 185~370mg/L、KH₂PO₄ 120~170mg/L、CaCl₂·2H₂O 220~440mg/L、KI 0.42~0.83mg/L、H₃BO₃ 3.1~6.2mg/L、MnSO₄·4H₂O 11.1~22.3mg/L、ZnSO₄·7H₂O 4.3~8.6mg/L、Na₂MoO₄·2H₂O 0.1~0.25mg/L、CuSO₄·5H₂O 0.01~0.025mg/L CoCl₂·6H₂O 0.01~0.025mg/L、FeSO₄·7H₂O 13.5~27.8mg/L、Na₂16.3-37.3 mg/L EDTA, 0.05-0.1 mg/L thiamine hydrochloride, 0.2-0.5 mg/L nicotinic acid, 0.3-0.5 mg/L pyridoxine hydrochloride, 1-2 mg/L glycine, and 50-100 mg/L inositol.

7. The method for rapid propagation of arrowroot according to claim 1, wherein in the step of S12, an induction medium is selected when adventitious bud induction culture is performed on the explant, and the induction medium further comprises 2.0-4.0 mg/L of 6-benzylaminopurine, 0.1-0.2 mg/L of naphthylacetic acid, 6.2g/L of carrageenan, and 30g/L of white sugar.

8. The method for rapid propagation of arrowroot according to claim 1, wherein in the step S13, an enrichment medium is selected for adventitious bud enrichment culture, and the enrichment medium further comprises 0.5-1.0 mg/L of 6-benzylaminopurine, 0.05-0.1 mg/L of naphthylacetic acid, 6.2g/L of carrageenan, and 30g/L of white sugar.

9. The method for rapid propagation of arrowroot in claim 1, wherein in the step S15, a rooting medium is selected in the rooting induction culture process, and the rooting medium further comprises 3g/L of activated carbon, 0.4-0.5 mg/L of naphthylacetic acid, 7g/L of carrageenan, and 20g/L of white sugar.