CN110465674A - A kind of preparation method of magnetic bi-layer gold nano grain - Google Patents
A kind of preparation method of magnetic bi-layer gold nano grain Download PDFInfo
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- CN110465674A CN110465674A CN201910914637.4A CN201910914637A CN110465674A CN 110465674 A CN110465674 A CN 110465674A CN 201910914637 A CN201910914637 A CN 201910914637A CN 110465674 A CN110465674 A CN 110465674A
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 39
- 229910052737 gold Inorganic materials 0.000 title claims abstract description 27
- 239000010931 gold Substances 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 39
- 239000011258 core-shell material Substances 0.000 claims abstract description 12
- VYFYYTLLBUKUHU-UHFFFAOYSA-N dopamine Chemical compound NCCC1=CC=C(O)C(O)=C1 VYFYYTLLBUKUHU-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000002105 nanoparticle Substances 0.000 claims abstract description 12
- 239000002245 particle Substances 0.000 claims abstract description 10
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical class [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- WTDHULULXKLSOZ-UHFFFAOYSA-N Hydroxylamine hydrochloride Chemical compound Cl.ON WTDHULULXKLSOZ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229920001503 Glucan Polymers 0.000 claims abstract description 6
- 229960003638 dopamine Drugs 0.000 claims abstract description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims abstract description 4
- FDWREHZXQUYJFJ-UHFFFAOYSA-M gold monochloride Chemical compound [Cl-].[Au+] FDWREHZXQUYJFJ-UHFFFAOYSA-M 0.000 claims abstract description 4
- NQXWGWZJXJUMQB-UHFFFAOYSA-K iron trichloride hexahydrate Chemical compound O.O.O.O.O.O.[Cl-].Cl[Fe+]Cl NQXWGWZJXJUMQB-UHFFFAOYSA-K 0.000 claims abstract description 3
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims description 14
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 9
- 229920002307 Dextran Polymers 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 6
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims description 5
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 5
- KIUMMUBSPKGMOY-UHFFFAOYSA-N 3,3'-Dithiobis(6-nitrobenzoic acid) Chemical compound C1=C([N+]([O-])=O)C(C(=O)O)=CC(SSC=2C=C(C(=CC=2)[N+]([O-])=O)C(O)=O)=C1 KIUMMUBSPKGMOY-UHFFFAOYSA-N 0.000 claims description 4
- 229910004042 HAuCl4 Inorganic materials 0.000 claims description 4
- 229910021577 Iron(II) chloride Inorganic materials 0.000 claims description 4
- FSVCELGFZIQNCK-UHFFFAOYSA-N N,N-bis(2-hydroxyethyl)glycine Chemical compound OCCN(CCO)CC(O)=O FSVCELGFZIQNCK-UHFFFAOYSA-N 0.000 claims description 4
- 239000007998 bicine buffer Substances 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- NMCUIPGRVMDVDB-UHFFFAOYSA-L iron dichloride Chemical compound Cl[Fe]Cl NMCUIPGRVMDVDB-UHFFFAOYSA-L 0.000 claims description 4
- SZVJSHCCFOBDDC-UHFFFAOYSA-N iron(II,III) oxide Inorganic materials O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 3
- AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 claims description 3
- 229910017912 NH2OH Inorganic materials 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 238000013019 agitation Methods 0.000 claims description 2
- 235000010323 ascorbic acid Nutrition 0.000 claims description 2
- 229960005070 ascorbic acid Drugs 0.000 claims description 2
- 239000011668 ascorbic acid Substances 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 239000006227 byproduct Substances 0.000 claims description 2
- 239000011259 mixed solution Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 239000002244 precipitate Substances 0.000 claims description 2
- 238000000746 purification Methods 0.000 claims description 2
- 229910001961 silver nitrate Inorganic materials 0.000 claims description 2
- 238000003786 synthesis reaction Methods 0.000 claims description 2
- WHNWPMSKXPGLAX-UHFFFAOYSA-N N-Vinyl-2-pyrrolidone Chemical compound C=CN1CCCC1=O WHNWPMSKXPGLAX-UHFFFAOYSA-N 0.000 claims 3
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims 2
- 239000005711 Benzoic acid Substances 0.000 claims 1
- 235000010233 benzoic acid Nutrition 0.000 claims 1
- 239000000047 product Substances 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 4
- 238000006555 catalytic reaction Methods 0.000 abstract description 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 2
- 150000001875 compounds Chemical class 0.000 abstract description 2
- 238000002595 magnetic resonance imaging Methods 0.000 abstract description 2
- 230000004043 responsiveness Effects 0.000 abstract description 2
- 239000011257 shell material Substances 0.000 description 6
- 239000011162 core material Substances 0.000 description 3
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 3
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 3
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 3
- 238000001069 Raman spectroscopy Methods 0.000 description 2
- 238000005253 cladding Methods 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 239000002122 magnetic nanoparticle Substances 0.000 description 2
- SLAMLWHELXOEJZ-UHFFFAOYSA-N 2-nitrobenzoic acid Chemical compound OC(=O)C1=CC=CC=C1[N+]([O-])=O SLAMLWHELXOEJZ-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 229940056319 ferrosoferric oxide Drugs 0.000 description 1
- 238000007306 functionalization reaction Methods 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 229910000510 noble metal Inorganic materials 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/06—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations
- A61K49/08—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by the carrier
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/06—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations
- A61K49/18—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes
- A61K49/1818—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles
- A61K49/1821—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles coated or functionalised microparticles or nanoparticles
- A61K49/1824—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles coated or functionalised microparticles or nanoparticles coated or functionalised nanoparticles
- A61K49/1827—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles coated or functionalised microparticles or nanoparticles coated or functionalised nanoparticles having a (super)(para)magnetic core, being a solid MRI-active material, e.g. magnetite, or composed of a plurality of MRI-active, organic agents, e.g. Gd-chelates, or nuclei, e.g. Eu3+, encapsulated or entrapped in the core of the coated or functionalised nanoparticle
- A61K49/183—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes particles, e.g. uncoated or non-functionalised microparticles or nanoparticles coated or functionalised microparticles or nanoparticles coated or functionalised nanoparticles having a (super)(para)magnetic core, being a solid MRI-active material, e.g. magnetite, or composed of a plurality of MRI-active, organic agents, e.g. Gd-chelates, or nuclei, e.g. Eu3+, encapsulated or entrapped in the core of the coated or functionalised nanoparticle having a (super)(para)magnetic core coated or functionalised with an inorganic material or being composed of an inorganic material entrapping the MRI-active nucleus, e.g. silica core doped with a MRI-active nucleus
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- B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
- B22F1/05—Metallic powder characterised by the size or surface area of the particles
- B22F1/054—Nanosized particles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
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- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
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Abstract
The present invention relates to a kind of preparation methods of magnetic bi-layer gold nano grain, belong to field of compound material.Method includes the following steps: preparing magnetic nanometer particles using certain density four water frerrous chloride, Iron trichloride hexahydrate, glucan and sodium hydroxide, and dopamine is added to generate magnetic core-shell plasma nano gap nanoparticle, finally with reagents such as gold chloride, hydroxylamine hydrochloride and cetyl trimethylammonium bromides, magnetic bi-layer gold nano grain is prepared.Magnetic bi-layer gold nano grain prepared by the present invention is because having good magnetic responsiveness, biocompatibility, the characteristics such as unique plasma resonance, therefore have important application prospect in the fields such as biotechnology, biosensor and biomedicine, magnetic resonance imaging and catalysis.
Description
Technical field
The present invention relates to a kind of preparation methods of magnetic bi-layer gold nano grain, belong to field of compound material.
Background technique
Multi-functional in order to realize single nanostructure, people conduct extensive research core-shell nano.Its
In, gold nano grain has many advantages, such as that chemical stability is high, reactivity is low, good biocompatibility, be using noble metal as
The core material of core-shell nano and the ideal material of shell material.Meanwhile passing through covalent bond and amine or Mercaptofunctional on the surface of golden shell
Group is combined the functionalization that may be implemented for golden shell.Magnetic nano-particle (Fe of the gold as shell material cladding3O4It NPs) can be with
The performance of the magnetic property of ferroso-ferric oxide and golden shell is combined, as one for biotechnology and biomedical applications
Suitable platform.
For the magnetic bi-layer gold nano grain that this patent is related to because having good magnetic responsiveness, biocompatibility is unique
Plasma resonance, and protecting so that core material improves from aoxidizing and corroding the chemistry of structure by outer bilayer golden shell
Stability, therefore have important application in the fields such as biotechnology, biosensor and biomedicine, magnetic resonance imaging and catalysis
Prospect.
Summary of the invention
The present invention improves in above method thinking, prepares the magnetic bi-layer gold nano grain with excellent performance；
Use certain density four water frerrous chloride (FeCl2·4H2O), Iron trichloride hexahydrate (FeCl3·6H2O), glucan
(Dextran) and sodium hydroxide (NaOH) prepares magnetic nanometer particles (Fe3O4- Dextran), and be added dopamine (PDA) with
Magnetic core-shell plasma nano gap nanoparticle is generated, gold chloride (HAuCl is finally used4), hydroxylamine hydrochloride (NH2OH·HCl)
And the reagents such as cetyl trimethylammonium bromide (CTAB), prepare magnetic bi-layer gold nano grain.
To achieve the above object, The technical solution adopted by the invention is as follows:
A kind of preparation method of magnetic bi-layer gold nano grain, comprising the following steps:
(1) magnetic nanometer particles (Fe3O4- Dextran) synthesis: four water frerrous chloride (FeCl2·4H2O), six water chlorine
Change iron (FeCl3·6H2O it) is dissolved in deionized water, under agitation gradually by mixed solution with glucan (Dextran)
Sodium hydroxide (NaOH) is added and stirs 2h, the black precipitate occurred is collected with magnet, is then washed with deionized 5 times；
(2) magnetic core-shell plasma nano gap nanoparticle: magnetic nanometer particles are dispersed in bicine buffering
In solution, dopamine (PDA) is then added to reach the thickness of required dopamine, after stirring 8h, by product centrifugal purification；
(3) magnetic core-shell plasma nano gap nanoparticle the preparation of Single Magnetic gold particle: is added to 10mL
Deionized water in, isometric gold chloride (HAuCl is added after stirring4) and hydroxylamine hydrochloride (NH2OH·HCl)；
(4) preparation of magnetic bi-layer gold nano grain: 5,5 '-two thiobis (2- are added in the Single Magnetic gold particle of preparation
Nitrobenzoic acid) (DTNB) and stir 3 hours, be centrifuged to 1mL.Sequentially add cetyl trimethylammonium bromide
(CTAB), polyvinylpyrrolidone (PVP), silver nitrate (AgNO3), ascorbic acid (L-AA) and NaOH solution, by mixture temperature
After educating 2 hours, HAuCl is added4Solution simultaneously heats.
FeCl described in above-mentioned steps (1)2·4H2O and FeCl3·6H2The molar ratio of O mixing is 1: 1-1: 5.
The dosage of the Dextran of addition described in above-mentioned steps (1) is 0.1-0.5g.
The concentration that NaOH is added described in above-mentioned steps (1) is 1-10mol/L.
Fe described in above-mentioned steps (1)3O4The dosage of glucan nanoparticle is 10-100 μ L.
The volume of bicine buffer solution described in above-mentioned steps (2) is 10-20mL.
The concentration that PDA is added described in above-mentioned steps (2) is 0.01-0.2mg/mL.
The dosage that magnetic core-shell plasma nano gap nanoparticle is added described in above-mentioned steps (3) is 0.1-1mL.
The mixing time in magnetic core-shell plasma nano gap nanoparticle described in above-mentioned steps (3) is 1-
10min。
Isometric HAuCl described in above-mentioned steps (3)4And NH2The volume of OHHCl is 1-10mL.
The HAuCl of addition described in above-mentioned steps (3)4Concentration be 2.5mmol/L, NH2The concentration of OHHCl is
The concentration of 0.2mol/L, DTNB are 1-10mmol/L, volume 0.1-10mL.
The concentration of the CTAB of addition described in above-mentioned steps (4) be 50-200mmol/L, volume 0.1-10mL, PVP's
Mass percent is 1-10%, volume 1-10mL, AgNO3Concentration be 1-10mmol/L, volume 0.1-10mL, L-AA's
Concentration is 100-500mmol/L, and the concentration of volume 0.1-10mL, NaOH are 100-500mmol/L, volume 0.1-10mL,
HAuCl4Concentration be 1-10mmol/L, volume 0.1-10mL, the temperature of heating is 80-150 DEG C, heating time 10-
30min。
Superiority of the invention:
A kind of synthetic method of magnetic bi-layer gold nano grain, it is total in biotechnology, biosensor and biomedicine, magnetic
There is important application in the fields such as vibration imaging and catalysis.Fe3O4The superparamagnetic characteristic of core material can be used for exterior guiding or as magnetic resonance at
As developer in (MRI).In recent years, the magnetic nanoparticle of gold cladding is a kind of ideal complex for combining nucleocapsid advantage
System has unique medical applications potentiality.
Detailed description of the invention
The following drawings is only intended to schematically illustrate the present invention and explain, however it is not limited to the scope of the present invention.Wherein:
Fig. 1 is the result photo of the embodiment of the present invention 1；
Fig. 2 is the result photo of the embodiment of the present invention 2；
Fig. 3 is the result photo of the embodiment of the present invention 3.
Specific embodiment
The present invention is described with specific embodiment with reference to the accompanying drawing.Unless stated otherwise, skill used in the present invention
Art means are method known in those skilled in the art.In addition, embodiment be interpreted as it is illustrative, rather than limit this
The range of invention, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, not
Under the premise of spirit and scope of the present invention, in these embodiments material component and dosage carry out various changes or
Change also belongs to protection scope of the present invention.
Embodiment 1:
The magnetic bi-layer gold nano grain of preparation is placed under transmission electron microscope and is observed, obtains TEM's as shown in Figure 1
Photo can be seen that the magnetic bi-layer gold nano grain after drying occurs comparing by the magnetism of itself institute's band from image
Apparent agglomeration.
Embodiment 2:
The magnetic bi-layer gold nano grain of preparation is placed in energy disperse spectroscopy (EDS) and is tested.It is tied as shown in Figure 2
Fruit, there are mainly three types of the components of the magnetic bi-layer gold nano grain of preparation, respectively gold, silver and iron, corresponding quality
Percentage is respectively 84.11%, 14.23% and 1.66%.
Embodiment 3:
The Raman detection of hand-held is put into after the magnetic bi-layer gold nano grain prepared drop is dried on silicon wafer
It is tested in instrument.It obtains as shown in Figure 3 as a result, the characteristic peak for the magnetic bi-layer gold nano grain prepared is located at 1331cm-1
Near, there is stronger Raman signal and good signal-to-noise ratio.
Claims (12)
1. a kind of preparation method of magnetic bi-layer gold nanorods, comprising the following steps:
(1) magnetic nanometer particles (Fe3O4- Dextran) synthesis: four water frerrous chloride (FeCl2·4H2O), Iron trichloride hexahydrate
(FeCl3·6H2O it) is dissolved in deionized water with glucan (Dextran), mixed solution is gradually added under agitation
Sodium hydroxide (NaOH) simultaneously stirs 2h, and the black precipitate occurred is collected with magnet, is then washed with deionized 5 times；
(2) magnetic core-shell plasma nano gap nanoparticle: magnetic nanometer particles are dispersed in bicine buffer solution
In, dopamine (PDA) is then added to reach the thickness of required dopamine, after stirring 8h, by product centrifugal purification；
(3) magnetic core-shell plasma nano gap nanoparticle the preparation of Single Magnetic gold particle: is added to going for 10mL
Isometric gold chloride (HAuCl is added in ionized water, after stirring4) and hydroxylamine hydrochloride (NH2OH·HCl)；
(4) preparation of magnetic bi-layer gold nano grain: 5,5 '-two thiobis (2- nitros are added in the Single Magnetic gold particle of preparation
Benzoic acid) (DTNB) and stir 3 hours, be centrifuged to 1mL.It sequentially adds cetyl trimethylammonium bromide (CTAB), gather
Vinylpyrrolidone (PVP), silver nitrate (AgNO3), ascorbic acid (L-AA) and NaOH solution, after mixture is incubated 2 hours,
HAuCl is added4Solution simultaneously heats.
2. a kind of preparation method of magnetic bi-layer gold nanorods according to claim 1, which is characterized in that above-mentioned steps (1)
Described in FeCl2·4H2O and FeCl3·6H2The molar ratio of O mixing is 1: 1-1: 5.
3. a kind of preparation method of magnetic bi-layer gold nanorods according to claim 1, which is characterized in that above-mentioned steps (1)
Described in addition Dextran dosage be 0.1-0.5g.
4. a kind of preparation method of magnetic bi-layer gold nanorods according to claim 1, which is characterized in that above-mentioned steps (1)
Described in addition NaOH concentration be 1-10mol/L.
5. a kind of preparation method of magnetic bi-layer gold nanorods according to claim 1, which is characterized in that above-mentioned steps (1)
Described in Fe3O4The dosage of glucan nanoparticle is 10-100 μ L.
6. a kind of preparation method of magnetic bi-layer gold nanorods according to claim 1, which is characterized in that above-mentioned steps (2)
Described in bicine buffer solution volume be 10-20mL.
7. a kind of preparation method of magnetic bi-layer gold nanorods according to claim 1, which is characterized in that above-mentioned steps (2)
Described in addition PDA concentration be 0.01-0.2mg/mL.
8. a kind of preparation method of magnetic bi-layer gold nanorods according to claim 1, which is characterized in that above-mentioned steps (3)
Described in addition magnetic core-shell plasma nano gap nanoparticle dosage be 0.1-1mL.
9. a kind of preparation method of magnetic bi-layer gold nanorods according to claim 1, which is characterized in that above-mentioned steps (3)
Described in magnetic core-shell plasma nano gap nanoparticle mixing time be 1-10min.
10. a kind of preparation method of magnetic bi-layer gold nanorods according to claim 1, which is characterized in that above-mentioned steps (3)
Described in isometric HAuCl4And NH2The volume of OHHCl is 1-10mL.
11. a kind of preparation method of magnetic bi-layer gold nanorods according to claim 1, which is characterized in that above-mentioned steps (3)
Described in HAuCl4Concentration be 2.5mmol/L, NH2The concentration of OHHCl is 0.2mol/L, and the concentration of DTNB is 1-
10mmol/L, volume 0.1-10mL.
12. a kind of preparation method of magnetic bi-layer gold nanorods according to claim 1, which is characterized in that above-mentioned steps (4)
Described in the concentration of CTAB be 50-200mmol/L, the mass percent of volume 0.1-10mL, PVP is 1-10%, volume
For 1-10mL, AgNO3Concentration be 1-10mmol/L, the concentration of volume 0.1-10mL, L-AA is 100-500mmol/L, body
Product is 0.1-10mL, and the concentration of NaOH is 100-500mmol/L, volume 0.1-10mL, HAuCl4Concentration be 1-10mmol/
L, volume 0.1-10mL, the temperature of heating are 80-150 DEG C, heating time 10-30min.
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