CN110463702B

CN110463702B - Application of hydroxyketone compound or preparation containing hydroxyketone compound in promoting plant root growth

Info

Publication number: CN110463702B
Application number: CN201910818694.2A
Authority: CN
Inventors: 骆世洪; 张桂山; 王安琪; 华娟
Original assignee: Shenyang Agricultural University
Current assignee: Shenyang Agricultural University
Priority date: 2019-08-30
Filing date: 2019-08-30
Publication date: 2021-03-26
Anticipated expiration: 2039-08-30
Also published as: CN110463702A

Abstract

The invention relates to an application of a hydroxyketone compound or a preparation containing the hydroxyketone compound in promoting the growth of plant roots, belonging to the technical field of agriculture. The application of hydroxyketone compounds or preparations containing the hydroxyketone compounds in promoting the growth of plant roots, wherein the hydroxyketone compounds are at least one of compounds shown in a formula I and a formula II,

Description

Application of hydroxyketone compound or preparation containing hydroxyketone compound in promoting plant root growth

Technical Field

The invention relates to an application of a hydroxyketone compound or a preparation containing the hydroxyketone compound in promoting the growth of plant roots, belonging to the technical field of agriculture.

Background

The plant root growth promoter is a substance which is artificially synthesized and has similar physiological and biological effects with plant hormone after people know the structure and action mechanism of natural plant hormone, is used in agricultural production, effectively promotes the growth process of crops, and achieves the purposes of stabilizing yield, increasing production, improving quality, enhancing stress resistance of crops and the like. In recent years, in order to obtain high-yield crops, vegetables and fruits, pesticides and chemical fertilizers are used in an overproof manner, so that chemical fertilizers and pesticide residues in agricultural products exceed standards, soil and water body pollution is caused, and a series of environmental damage and ecological disasters are caused. Seriously threatens the survival and development of human beings, and is an ideal way to develop a novel multifunctional plant growth promoter.

Disclosure of Invention

The invention aims to provide an application of a hydroxyketone compound or a preparation containing the hydroxyketone compound in promoting the growth of plant roots, and also provides a plant root growth promoter, which effectively promotes the growth and development process of crops, and achieves the effects of stabilizing yield, increasing production, improving quality and the like.

The application of hydroxyketone compounds or preparations containing the hydroxyketone compounds in promoting the growth of plant roots, wherein the hydroxyketone compounds are at least one of compounds shown in a formula I and a formula II,

the preparation containing the hydroxyketone compound takes the hydroxyketone compound as an active component and also comprises auxiliary materials. The above hydroxyketone compound can be used alone or in combination with other adjuvants in the growth process of plant roots.

The hydroxyketone compound or the preparation containing the hydroxyketone compound has at least one of the following functions in promoting the growth of plant seedlings:

promoting the growth of the roots of the plant seedlings;

promoting the increase of the weight of the roots of the plant seedlings.

The hydroxy ketone compound is composed of compounds shown in a formula I and a formula II, and further is composed of compounds shown in the formula I and the formula II according to the mass ratio of 1: 10-10: 1.

Furthermore, the hydroxyketone compound is composed of compounds shown in a formula I and a formula II according to a mass ratio of 2: 3.

Further, the plant is corn, rice, cucumber or tomato.

The hydroxyketone compounds shown in formula I and formula II are prepared from fermentation cultures of bacillus WRN 031.

The Bacillus subtilis WRN031 of the method can be obtained by an Agricultural microbial strain preservation management center (ACCC) of China, with a preservation number ACCC 19993; and can be purchased commercially by agricultural resources and agricultural district research institutes of Chinese agricultural academy of sciences.

Further preferably, the hydroxyketones of formula I and formula II are prepared as follows: the bacillus subtilis is obtained by extracting fermentation culture solution of bacillus with ethyl acetate and then separating the ethyl acetate extract through silica gel column chromatography, wherein the silica gel column chromatography adopts petroleum ether and acetone as elution conditions, and the acetone is 2: 1.

Another object of the present invention is to provide a plant root growth promoter.

A plant root growth promoter comprises active component of hydroxyketone compound, wherein the hydroxyketone compound is at least one of compounds shown in formula I and formula II,

further, the promoter has at least one of the following functions:

promoting the growth of the roots of the plant seedlings;

promoting the increase of the weight of the roots of the plant seedlings.

Further, the active ingredient of the accelerant is composed of compounds shown in a formula I and a formula II according to the mass ratio of 1: 10-10: 1.

Furthermore, the active component of the accelerator consists of compounds shown in a formula I and a formula II according to a mass ratio of 2: 3.

The accelerator disclosed by the invention preferably consists of a hydroxyketone compound and auxiliary materials.

Further, the accelerant consists of the following components in percentage by mass: 0.5-15% of hydroxyl ketone compound, 0.2-2% of cosolvent, 0.1-3% of preservative, 1-10% of auxiliary material assistant and the balance of water.

Wherein the cosolvent is one or two of sodium benzoate, sodium salicylate, p-aminobenzoic acid, tween 20 and tween 80; the preservative is one or more of benzoic acid, sorbic acid, dehydroacetic acid, sodium diacetate, calcium acetate, calcium propionate, phosphate, ethyl p-hydroxybenzoate and propyl p-hydroxybenzoate; the auxiliary material auxiliary agent is one or more of alkylphenol polyoxyethylene, alkylbenzene sulfonate, naphthalene sulfonate, a sulfonate formaldehyde condensate, castor oil polyoxyethylene polyoxypropylene ether, fatty acid polyoxyethylene ester phosphate and alkyl polyoxyethylene ether phosphate.

It is another object of the present invention to provide a method for promoting the growth of young plants.

A method of promoting the growth of young plants by applying the plant root promoter to plant seeds or young plants.

The active component of the accelerant is a hydroxyketone compound, the hydroxyketone compound is at least one of compounds with a formula I and a formula II,

furthermore, the application amount of the seedling growth promoter is 0.04-16 mu g per plant in terms of hydroxyl ketone compounds.

Furthermore, the application amount of the seedling growth promoter is 0.08-16 mu g per plant based on the hydroxyketone compound.

The activity test shows that the plant root growth promoter of the invention has obvious promotion effect on the roots of corn, rice, cucumber and tomato. In practical use, the composition of the plant root growth promoting compounds I and II of the present invention is applied to the target plants in the following amounts: the amount ranges from 0.04 to 16 mu g per strain, and the combined mass ratio of the compound I to the compound II is 1:10 to 10: 1; the plant root growth promoter of the present invention, the compound I or II monomer, is applied to the target plant in the following amount: the amount is in the range of 0.04-16 mu g per strain. The plant root growth promoter of the invention can act on the roots of plants, thereby achieving the effects of increasing the yield, improving the quality and the like.

The invention has the beneficial effects that: the method is simple and low in cost, and the plant root growth promoter can obviously promote the growth of plant roots and improve the growth capacity of plants in the seedling stage, thereby achieving the purpose of increasing yield.

Detailed Description

The following non-limiting examples are presented to enable those of ordinary skill in the art to more fully understand the present invention and are not intended to limit the invention in any way.

The test methods described in the following examples are all conventional methods unless otherwise specified; the reagents and materials are commercially available, unless otherwise specified.

Isolation and characterization of Compounds of formula I and formula II (hereinafter referred to simply as Compound I and Compound II) used in the examples described below

Bacillus WRN031 (commercially available from institute of agricultural resources and agricultural divisions of Chinese academy of agricultural sciences) was cultured in Landy medium and fermented at 37 deg.C for 36h to obtain 100L fermentation broth. And extracting the fermentation liquor by using ethyl acetate to obtain 80g of ethyl acetate extract. The ethyl acetate extract was separated by silica gel column chromatography, eluting with petroleum ether and acetone 2:1 to give compound I8g (> 95% pure) and compound II12g (> 93% pure), respectively.

The 1D NMR data for compounds I and II are as follows:

a compound I:¹H-NMR(600MHz，CDCl₃)，δ2.23(3H，s，H-1)，1.42(3H，s，3-CH₃)，3.85(1H，q，J＝6.4Hz，H-4)，1.06(3H，d，J＝6.4Hz，H-5)；¹³C-NMR(150MHz，CDCl₃)，δ211.0(s，C-2)，81.4(s，C-3)，71.2(d，C-4)，25.0(q，C-5)，21.8(q，C-1)，17.9(q，3-CH₃)。

compound II:¹H-NMR(600MHz，CDCl₃)，δ2.29(3H，s，H-1)，1.26(3H，s，3-CH₃)，4.03(1H，q，J＝6.3Hz，H-4)，1.28(3H，d，J＝6.3Hz，H-5)；¹³C-NMR(150MHz，CDCl₃)，δ211.5(s，C-2)，81.4(s，C-3)，71.0(d，C-4)，23.8(q，C-5)，21.5(q，C-1)，16.9(q，3-CH₃)。

example 1 promoting activity of different amounts of compound I and II compositions (compound I: II ═ 2:3, m/m) on corn root growth

The compositions of compounds I and II (compound I: II ═ 2:3, m/m) were tested for their corn root growth promoting activity by the sand culture method at different application rates. The method comprises the steps of firstly sterilizing sand for 3 times continuously under the condition of 30min by using a high-temperature sterilizing pot, sterilizing corn seeds (84 sterilizing liquid: water is 3:7) for 10-12min, then cleaning the corn seeds for 4-5 times by using sterile water, planting the corn seeds into pots containing about 700g of sand (sterilization), culturing the corn seeds in a culture room at 28 ℃ in 5 seeds in each pot, applying different amounts of compound I and compound II compositions (2:3, m/m) to germinated corn plants after the plants germinate, wherein the application amount of the compound I and compound II compositions is 0, 0.04, 0.08, 0.4, 0.8, 4, 8 and 16 mu g/plant, three times are set for each application amount, and 0 mu g/plant is a control group (CK). The root length of the corn was measured on day 7.

The results show that the compositions of compounds I and II (2:3, m/m) applied at different amounts have growth promoting effect on the roots of corn and show a certain concentration increase dependence.

TABLE 1 corn root promoting Activity of different application amounts of Compound I and II compositions (2:3, m/m)

Application amount	Root length (cm)	SD(cm)
			0 μ g/strain (CK)	13.86	0.45
0.04 μ g/strain	14.03	1.11
			0.08. mu.g/strain	13.92	0.78
0.4. mu.g/strain	15.67	1.28
			0.8. mu.g/strain	16.61	2.32
4 μ g/strain	19.43	1.57
			8 mu g/strain	18.64	0.97
16 μ g/strain	17.95	0.72

EXAMPLE 2 corn root growth promoting Activity of different ratio combinations of Compounds I and II

The sand culture method described in example 1 was used to test the growth promoting activity of corn roots by using compositions of compounds I and II (compound I: II: 1: 10-10: 1) in different proportions, and the application amount of the composition was 4 μ g/strain. As a result, it was found that all the ratios promoted the growth of corn roots, but the root growth promoting activity of compound I: II ═ 2:3 was better.

TABLE 2 growth promoting Activity of different proportions of the compositions of Compounds I and II on corn roots (application amount 4. mu.g/strain)

EXAMPLE 3 growth promoting Activity of Compounds I and II monomers on corn root

The compounds I and II were each applied in the gradient described in example 1, and the growth-promoting effect of the individual compounds on the corn roots was determined by the sand culture method in example 1 at different application rates. The results show that the monomers of the compounds I and II have obvious promotion effect on the roots of the corn.

TABLE 3 corn root promoting Activity of Compound I

Application amount	Root length (cm)	SD(cm)
			0 μ g/strain (CK)	14.76	0.78
0.08. mu.g/strain	15.66	0.43
			0.4. mu.g/strain	16.82	1.76
0.8. mu.g/strain	17.83	1.21
			4 μ g/strain	18.33	1.57
8 mu g/strain	18.41	1.45

TABLE 4 corn root promoting Activity of Compound II

Application amount	Root length (cm)	SD(cm)
			0 μ g/strain (CK)	12.61	2.72
0.08. mu.g/strain	13.63	2.66
			0.4. mu.g/strain	14.54	1.82
0.8. mu.g/strain	15.35	2.75
			4 μ g/strain	17.65	3.69
8 mu g/strain	18.34	1.68

EXAMPLE 4 promoting Activity of different amounts of Compound I and II compositions (2:3, m/m) on cucumber root growth

According to the experimental method of sand culture in example 1, different amounts of a combination of compounds I and II (2:3, m/m) were applied to the roots of cucumber seedlings, and the length of the roots of the cucumbers was measured after 15 days. Test results show that the composition (2:3, m/m) of the compounds I and II has obvious promotion effect on the growth of cucumber roots.

TABLE 5 cucumber root promoting Activity of the combination of Compounds I and II (2:3, m/m)

Application amount	Root length (cm)	SD(cm)
			0 μ g/strain (CK)	5.02	1.23
0.08. mu.g/strain	5.78	0.97
			0.4. mu.g/strain	5.96	1.18
0.8. mu.g/strain	6.45	0.54
			4 μ g/strain	6.89	0.82
8 mu g/strain	7.43	1.01

EXAMPLE 5 cucumber root growth promoting Activity of Compounds I and II monomers

According to the experimental method of sand culture in example 1, different amounts of monomers of compounds I and II were applied to the roots of cucumber seedlings, and the length of the roots of the cucumber was measured 15 days later. Test results show that the compounds I and II have obvious root growth promoting effect on cucumbers.

TABLE 6 growth promoting Activity of Compound I on cucumber roots

Application amount	Root length (cm)	SD(cm)
			0 μ g/strain (CK)	4.89	0.78
0.08. mu.g/strain	5.23	0.43
			0.4. mu.g/strain	5.84	1.26
0.8. mu.g/strain	6.66	0.79
			4 μ g/strain	7.01	0.76
8 mu g/strain	7.238	0.54

TABLE 7 growth promoting Activity of Compound II on cucumber roots

Application amount	Root length (cm)	SD(cm)
			0 μ g/strain (CK)	5.18	0.67
0.08. mu.g/strain	5.20	0.74
			0.4. mu.g/strain	5.82	0.32
0.8. mu.g/strain	6.34	0.57
			4 μ g/strain	6.89	0.79
8 mu g/strain	7.69	0.41

EXAMPLE 6 accelerating Activity of different application amounts of Compound I and II compositions (2:3, m/m) on tomato root growth

According to the experimental method of sand culture in example 1, different amounts of the combination of compounds I and II (2:3, m/m) were applied to the roots of tomato seedlings and the length of the roots of the tomatoes was measured after 15 days. The test results show that the composition of the compounds I and II (2:3, m/m) has obvious promotion effect on the growth of tomato roots.

TABLE 8 growth promoting Activity of compositions of Compounds I and II (2:3, m/m) on tomato roots

Application amount	Root length (cm)	SD(cm)
			0 μ g/strain (CK)	6.99	0.78
0.08. mu.g/strain	7.04	0.43
			0.4μg/strain	7.65	1.06
0.8. mu.g/strain	8.49	1.02
			4 μ g/strain	8.93	0.59
8 mu g/strain	9.84	0.45

EXAMPLE 7 promoting Activity of Compounds I and II monomers on tomato root growth

According to the experimental method of sand culture in example 1, different amounts of the monomers of compounds I and II were applied to the roots of tomato seedlings, and the length of the roots of the tomatoes was measured after 15 days. The test results show that the composition of the compounds I and II (2:3, m/m) has obvious promotion effect on the growth of tomato roots.

TABLE 9 growth promoting Activity of Compound I on tomato roots

Application amount	Root length (cm)	SD(cm)
			0 μ g/strain (CK)	7.76	0.78
0.08. mu.g/strain	8.03	0.44
			0.4. mu.g/strain	8.69	0.97
0.8. mu.g/strain	9.42	1.21
			4 μ g/strain	9.89	0.57
8 mu g/strain	10.54	1.01

TABLE 10 growth promoting Activity of Compound II on tomato roots

EXAMPLE 8 growth promoting Activity of different application amounts of Compound I and II compositions (2:3, m/m) on Rice roots

The method adopted is a seed bag culture method, firstly, rice seeds are placed in a refrigerator at 4 ℃ for vernalization for 3d, the vernalized rice seeds (84 disinfectant solution: water: 3:7) are disinfected for 10-12min, then are washed with sterile water for 4-5 times, and the rice seeds are planted in seed bags containing about 30mL of solution, so that the application amount of the compound I and II composition in the seed bags is 0, 0.08, 0.4, 0.8, 4 and 8 mu g/plant, and 0 mu g/plant is used as a control group (CK). 12 seeds were placed in each bag and incubated in a 28 ℃ incubation chamber at triplicate for each concentration, water was used as a control. The growth of the cells was observed for 7 days, and the root length, root weight, overground length and overground weight were measured. Test results show that the composition (2:3, m/m) of the compounds I and II has obvious root growth promoting effect on rice.

TABLE 11 growth promoting Activity of the combination of Compounds I and II (2:3, m/m) on Rice roots

Application amount	Root length (cm)	SD(cm)
			0 μ g/strain (CK)	15.37	0.46
0.08. mu.g/strain	14.04	1.14
			0.4. mu.g/strain	15.58	0.844
0.8. mu.g/strain	17.11	0.425
			4 μ g/strain	17.76	0.488
8 mu g/strain	18.37	0.95

EXAMPLE 9 Rice root growth promoting Activity of Compounds I and II monomers

The growth-promoting effect of the monomers of the compounds I and II on rice was determined by the seed bag culture method in example 8, and the compositions of the compounds I and II were applied at 0, 0.08, 0.4, 0.8, 4 and 8. mu.g/strain, and 0. mu.g/strain was used as a control group (CK). The length of the roots of rice was measured 7 days later. Test results show that the compounds I and II have obvious root growth promoting effect on rice.

TABLE 12 growth promoting Activity of Compound I on Rice roots

TABLE 13 growth promoting Activity of Compound II on Rice roots

Application amount	Root length (cm)	SD(cm)
			0 μ g/strain (CK)	13.74	0.85
0.08. mu.g/strain	13.39	2.13
			0.4. mu.g/strain	14.96	0.77
0.8. mu.g/strain	15.69	0.84
			4 μ g/strain	16.13	0.48
8 mu g/strain	17.46	1.30

EXAMPLE 10 preparation of Compound I and II compositions plant growth promoters

The plant growth promoter prepared from the compound I and the compound II comprises the following components in percentage by mass: 0.5-15% of compound I and compound II composition, 0.2-2% of cosolvent, 0.1-3% of preservative, 1-10% of auxiliary material auxiliary agent and the balance of water.

Formula 1: the composition 1:2 of the compounds I and II comprises 1 percent of sodium salicylate, 0.5 percent of sodium benzoate, 1 percent of sodium benzoate, 6 percent of alkylphenol polyoxyethylene and the balance of water.

And (2) formula: 5 percent of compound I and compound II composition 1:5, 1 percent of p-aminobenzoic acid, 0.2 percent of sodium benzoate, 10 percent of alkylphenol polyoxyethylene and the balance of water.

And (3) formula: the composition 2:3 of the compounds I and II is 8%, the Tween 20 is 2%, the dehydroacetic acid is 1%, the alkyl polyoxyethylene ether phosphate ester is 3%, and the balance of water.

And (4) formula: compound I and II composition 3:2 was 8%, tween 80 was 1%, calcium acetate 0.7%, sulfonate formaldehyde condensate, balance water.

And (5) formula: composition 5:1 of compound I and II is 10%, ethyl p-hydroxybenzoate 1%, sodium benzoate 0.5%, alkylbenzene sulfonate 6%, and balance water.

And (6) formula: compound I and II composition 4:3 was 15%, propyl paraben 0.5%, tween 80 was 1%, castor oil polyoxyethylene polyoxypropylene ether 8%, and balance water.

Example 11 application of the plant growth promoter of the compound I and II compositions in the aspect of promoting the growth of rice seedlings the plant growth promoter of the compound I and II compositions was obtained according to the formulations 1 to 6 in example 10. Germinating and growing seedlings of Pioneer No. 8 rice, transplanting the seedlings to a test field, spraying the plant growth promoters of the compound I and compound II compositions of the formula 1-6 according to a dose of 20 g/mu (about 1mg of promoter per plant), setting a control (not spraying), and after spraying for 10 days, selecting 10 seedlings to calculate the root length (centimeter, cm).

TABLE 14 plant growth promoting Activity of Compound I and II compositions on Rice seedlings in test fields

Compound I and II composition formula	Root length (cm)	SD(cm)
			Control	12.56	1.23
Formulation 1	15.92	0.89
			Formulation 2	14.38	2.35
Formulation 3	17.26	1.01
			Formulation 4	16.37	1.32
Formulation 5	18.55	0.56

EXAMPLE 12 monomeric plant growth promoter preparation of Compounds I and II

The plant growth promoter prepared from the monomers of the compounds I and II comprises the following components in percentage by mass: 0.5-15% of monomers of the compounds I and II, 0.2-2% of cosolvent, 0.1-3% of preservative, 1-10% of auxiliary material auxiliary agent and the balance of water.

Formula 1: 0.5% of monomer of compound I, 2% of sodium salicylate, 2% of sodium benzoate, 4% of alkylphenol polyoxyethylene and the balance of water.

And (2) formula: 2% of monomer of compound I, 0.5% of p-aminobenzoic acid, 0.2% of sorbic acid, 5% of alkylbenzene sulfonate and the balance of water.

And (3) formula: 5% of monomer of compound I, 2% of Tween 20, 1% of dehydroacetic acid, 5% of naphthalene sulfonate and the balance of water.

And (4) formula: 8% of monomer of compound I, 2% of Tween 80, 1% of calcium propionate, 10% of castor oil polyoxyethylene polyoxypropylene ether and the balance of water.

And (5) formula: 10% of monomer of compound I, 1% of Tween 20, 0.5% of sodium benzoate, 2% of propyl p-hydroxybenzoate, 3% of fatty acid polyoxyethylene ester phosphate and the balance of water.

And (6) formula: 1% of monomer of compound II, 1% of Tween 80, 0.5% of propyl p-hydroxybenzoate, 8% of alkylphenol polyoxyethylene and the balance of water.

And (3) formula 7: 3% of monomer of compound II, 1% of sodium salicylate, 0.5% of ethyl p-hydroxybenzoate, 2% of castor oil polyoxyethylene polyoxypropylene ether and the balance of water.

And (4) formula 8: 5% of monomer of compound II, 0.5% of sodium benzoate, 1% of sorbic acid, 2% of fatty acid polyoxyethylene ester phosphate and the balance of water.

Formula 9: 10% of monomer of compound II, 1% of Tween 80, 2% of sodium salicylate, 0.5% of propyl p-hydroxybenzoate, 8% of castor oil polyoxyethylene polyoxypropylene ether and the balance of water.

Formula 10: 15% of monomer of compound II, 1% of Tween 20, 0.2% of propyl p-hydroxybenzoate, 1% of calcium propionate, 5% of alkylbenzene sulfonate and the balance of water.

EXAMPLE 13 use of monomeric plant growth promoters of Compounds I and II in the promotion of Rice seedling growth

The monomeric plant growth promoter of the compound I and II is obtained according to the formula 1-10 in the embodiment 12. Germinating and growing seedlings of Pioneer No. 8 rice, transplanting the seedlings to a test field, spraying the compound I and II monomer plant growth promoters with the formulas 1-6 according to the dose of 20 g/mu (about 1mg promoter/plant), setting a control (not spraying), and after spraying for 10 days, selecting 10 seedlings to calculate the root length (centimeter, cm).

TABLE 15 promoting Activity of monomeric plant growth promoters of Compounds I and II on Rice seedlings in test fields

From the above experimental results, it can be seen that the compounds I and II of the present invention can significantly increase the root length of plants. The above embodiments are merely illustrative, and not restrictive, and those skilled in the art can make various changes and modifications without departing from the spirit and scope of the invention. Therefore, all equivalent technical solutions also belong to the scope of the present invention, and the protection scope of the present invention should be defined by the claims.

Claims

1. The application of the hydroxyketone compound or the preparation containing the hydroxyketone compound in promoting the growth of plant roots is characterized in that: the hydroxyketone compound is at least one of a compound shown in a formula I and a compound shown in a formula II,

2. use according to claim 1, characterized in that: the hydroxyketone compound is composed of compounds shown in a formula I and a formula II according to a mass ratio of 1: 10-10: 1.

3. Use according to claim 1, characterized in that: the plant is corn, rice, cucumber or tomato.

4. Use according to claim 1, characterized in that: the hydroxyketone compound is prepared from a fermentation culture of bacillus.

5. Use according to claim 1, characterized in that: the preparation containing the hydroxyl ketone compound comprises the following components in percentage by mass: 0.5-15% of hydroxyl ketone compound, 0.2-2% of cosolvent, 0.1-3% of preservative, 1-10% of auxiliary material auxiliary agent and the balance of water,

6. Use according to claim 1 or 5, characterized in that: applying a hydroxyketone compound or a preparation containing a hydroxyketone compound to a plant seed or a plant seedling.

7. Use according to claim 6, characterized in that: the application amount of the hydroxyketone compound or the preparation containing the hydroxyketone compound is 0.04-16 mu g/strain based on the hydroxyketone compound.