CN110463702B - Application of hydroxyketone compound or preparation containing hydroxyketone compound in promoting plant root growth - Google Patents
Application of hydroxyketone compound or preparation containing hydroxyketone compound in promoting plant root growth Download PDFInfo
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- CN110463702B CN110463702B CN201910818694.2A CN201910818694A CN110463702B CN 110463702 B CN110463702 B CN 110463702B CN 201910818694 A CN201910818694 A CN 201910818694A CN 110463702 B CN110463702 B CN 110463702B
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- -1 hydroxyketone compound Chemical class 0.000 title claims abstract description 64
- 241000196324 Embryophyta Species 0.000 title claims abstract description 41
- 230000001737 promoting effect Effects 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 230000002786 root growth Effects 0.000 title abstract description 24
- 150000001875 compounds Chemical class 0.000 claims abstract description 91
- 230000008635 plant growth Effects 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 235000007164 Oryza sativa Nutrition 0.000 claims description 21
- 240000008042 Zea mays Species 0.000 claims description 21
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 21
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 21
- 235000005822 corn Nutrition 0.000 claims description 21
- 235000009566 rice Nutrition 0.000 claims description 21
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 20
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 15
- 229910019142 PO4 Inorganic materials 0.000 claims description 15
- 235000021317 phosphate Nutrition 0.000 claims description 15
- 239000010452 phosphate Substances 0.000 claims description 15
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 14
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 14
- 240000003768 Solanum lycopersicum Species 0.000 claims description 14
- ALYNCZNDIQEVRV-UHFFFAOYSA-N 4-aminobenzoic acid Chemical compound NC1=CC=C(C(O)=O)C=C1 ALYNCZNDIQEVRV-UHFFFAOYSA-N 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 11
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims description 11
- 239000004299 sodium benzoate Substances 0.000 claims description 11
- 235000010234 sodium benzoate Nutrition 0.000 claims description 11
- QELSKZZBTMNZEB-UHFFFAOYSA-N propylparaben Chemical compound CCCOC(=O)C1=CC=C(O)C=C1 QELSKZZBTMNZEB-UHFFFAOYSA-N 0.000 claims description 10
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 9
- 229920000053 polysorbate 80 Polymers 0.000 claims description 9
- 239000004405 propyl p-hydroxybenzoate Substances 0.000 claims description 9
- 235000010232 propyl p-hydroxybenzoate Nutrition 0.000 claims description 9
- 240000008067 Cucumis sativus Species 0.000 claims description 8
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 8
- ABBQHOQBGMUPJH-UHFFFAOYSA-M Sodium salicylate Chemical compound [Na+].OC1=CC=CC=C1C([O-])=O ABBQHOQBGMUPJH-UHFFFAOYSA-M 0.000 claims description 8
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 8
- 239000004359 castor oil Substances 0.000 claims description 8
- 235000019438 castor oil Nutrition 0.000 claims description 8
- 239000006184 cosolvent Substances 0.000 claims description 8
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 claims description 8
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 8
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 8
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 8
- 229920002503 polyoxyethylene-polyoxypropylene Polymers 0.000 claims description 8
- 239000003755 preservative agent Substances 0.000 claims description 8
- 230000002335 preservative effect Effects 0.000 claims description 8
- 229960004025 sodium salicylate Drugs 0.000 claims description 8
- 239000012752 auxiliary agent Substances 0.000 claims description 7
- BCZXFFBUYPCTSJ-UHFFFAOYSA-L Calcium propionate Chemical compound [Ca+2].CCC([O-])=O.CCC([O-])=O BCZXFFBUYPCTSJ-UHFFFAOYSA-L 0.000 claims description 6
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 6
- 239000004287 Dehydroacetic acid Substances 0.000 claims description 6
- 229960004050 aminobenzoic acid Drugs 0.000 claims description 6
- 239000004330 calcium propionate Substances 0.000 claims description 6
- 235000010331 calcium propionate Nutrition 0.000 claims description 6
- 235000019258 dehydroacetic acid Nutrition 0.000 claims description 6
- 229940061632 dehydroacetic acid Drugs 0.000 claims description 6
- JEQRBTDTEKWZBW-UHFFFAOYSA-N dehydroacetic acid Chemical compound CC(=O)C1=C(O)OC(C)=CC1=O JEQRBTDTEKWZBW-UHFFFAOYSA-N 0.000 claims description 6
- PGRHXDWITVMQBC-UHFFFAOYSA-N dehydroacetic acid Natural products CC(=O)C1C(=O)OC(C)=CC1=O PGRHXDWITVMQBC-UHFFFAOYSA-N 0.000 claims description 6
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 6
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 claims description 6
- 239000004403 ethyl p-hydroxybenzoate Substances 0.000 claims description 6
- 235000010228 ethyl p-hydroxybenzoate Nutrition 0.000 claims description 6
- 229940043351 ethyl-p-hydroxybenzoate Drugs 0.000 claims description 6
- NUVBSKCKDOMJSU-UHFFFAOYSA-N ethylparaben Chemical compound CCOC(=O)C1=CC=C(O)C=C1 NUVBSKCKDOMJSU-UHFFFAOYSA-N 0.000 claims description 6
- 239000000194 fatty acid Substances 0.000 claims description 6
- 229930195729 fatty acid Natural products 0.000 claims description 6
- 150000004665 fatty acids Chemical class 0.000 claims description 6
- 239000004334 sorbic acid Substances 0.000 claims description 6
- 235000010199 sorbic acid Nutrition 0.000 claims description 6
- 229940075582 sorbic acid Drugs 0.000 claims description 6
- 125000000217 alkyl group Chemical group 0.000 claims description 5
- VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 claims description 5
- 239000001639 calcium acetate Substances 0.000 claims description 5
- 235000011092 calcium acetate Nutrition 0.000 claims description 5
- 229960005147 calcium acetate Drugs 0.000 claims description 5
- 238000000855 fermentation Methods 0.000 claims description 5
- 230000004151 fermentation Effects 0.000 claims description 5
- PSZYNBSKGUBXEH-UHFFFAOYSA-M naphthalene-1-sulfonate Chemical compound C1=CC=C2C(S(=O)(=O)[O-])=CC=CC2=C1 PSZYNBSKGUBXEH-UHFFFAOYSA-M 0.000 claims description 5
- 229940051841 polyoxyethylene ether Drugs 0.000 claims description 5
- 229920000056 polyoxyethylene ether Polymers 0.000 claims description 5
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 claims description 5
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 4
- 239000005711 Benzoic acid Substances 0.000 claims description 4
- 235000010233 benzoic acid Nutrition 0.000 claims description 4
- 229960004365 benzoic acid Drugs 0.000 claims description 4
- OPGYRRGJRBEUFK-UHFFFAOYSA-L disodium;diacetate Chemical compound [Na+].[Na+].CC([O-])=O.CC([O-])=O OPGYRRGJRBEUFK-UHFFFAOYSA-L 0.000 claims description 4
- 239000001632 sodium acetate Substances 0.000 claims description 4
- 229960003885 sodium benzoate Drugs 0.000 claims description 4
- 235000017454 sodium diacetate Nutrition 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims 1
- 239000007952 growth promoter Substances 0.000 abstract description 23
- 230000012010 growth Effects 0.000 abstract description 17
- 238000000034 method Methods 0.000 abstract description 10
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical class OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 abstract description 9
- 239000000203 mixture Substances 0.000 description 39
- 239000000178 monomer Substances 0.000 description 22
- 241000209094 Oryza Species 0.000 description 20
- HTSGKJQDMSTCGS-UHFFFAOYSA-N 1,4-bis(4-chlorophenyl)-2-(4-methylphenyl)sulfonylbutane-1,4-dione Chemical compound C1=CC(C)=CC=C1S(=O)(=O)C(C(=O)C=1C=CC(Cl)=CC=1)CC(=O)C1=CC=C(Cl)C=C1 HTSGKJQDMSTCGS-UHFFFAOYSA-N 0.000 description 18
- 101000993347 Gallus gallus Ciliary neurotrophic factor Proteins 0.000 description 18
- 238000012360 testing method Methods 0.000 description 12
- 230000000694 effects Effects 0.000 description 11
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 9
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 9
- 239000004576 sand Substances 0.000 description 9
- HEDRZPFGACZZDS-MICDWDOJSA-N Trichloro(2H)methane Chemical compound [2H]C(Cl)(Cl)Cl HEDRZPFGACZZDS-MICDWDOJSA-N 0.000 description 8
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 6
- 244000305550 Streptopus amplexifolius Species 0.000 description 6
- 235000001231 Streptopus amplexifolius Nutrition 0.000 description 6
- 238000009472 formulation Methods 0.000 description 6
- 238000005507 spraying Methods 0.000 description 6
- 238000012136 culture method Methods 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
- 239000002024 ethyl acetate extract Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000010898 silica gel chromatography Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 2
- 238000005160 1H NMR spectroscopy Methods 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 235000009849 Cucumis sativus Nutrition 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000003208 petroleum Substances 0.000 description 2
- 239000003375 plant hormone Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000000087 stabilizing effect Effects 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 241001057636 Dracaena deremensis Species 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000238 one-dimensional nuclear magnetic resonance spectroscopy Methods 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 229960003415 propylparaben Drugs 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
- C07C45/78—Separation; Purification; Stabilisation; Use of additives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
- C07C45/78—Separation; Purification; Stabilisation; Use of additives
- C07C45/79—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
- C07C45/78—Separation; Purification; Stabilisation; Use of additives
- C07C45/80—Separation; Purification; Stabilisation; Use of additives by liquid-liquid treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
- C12P7/26—Ketones
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to an application of a hydroxyketone compound or a preparation containing the hydroxyketone compound in promoting the growth of plant roots, belonging to the technical field of agriculture. The application of hydroxyketone compounds or preparations containing the hydroxyketone compounds in promoting the growth of plant roots, wherein the hydroxyketone compounds are at least one of compounds shown in a formula I and a formula II,
Description
Technical Field
The invention relates to an application of a hydroxyketone compound or a preparation containing the hydroxyketone compound in promoting the growth of plant roots, belonging to the technical field of agriculture.
Background
The plant root growth promoter is a substance which is artificially synthesized and has similar physiological and biological effects with plant hormone after people know the structure and action mechanism of natural plant hormone, is used in agricultural production, effectively promotes the growth process of crops, and achieves the purposes of stabilizing yield, increasing production, improving quality, enhancing stress resistance of crops and the like. In recent years, in order to obtain high-yield crops, vegetables and fruits, pesticides and chemical fertilizers are used in an overproof manner, so that chemical fertilizers and pesticide residues in agricultural products exceed standards, soil and water body pollution is caused, and a series of environmental damage and ecological disasters are caused. Seriously threatens the survival and development of human beings, and is an ideal way to develop a novel multifunctional plant growth promoter.
Disclosure of Invention
The invention aims to provide an application of a hydroxyketone compound or a preparation containing the hydroxyketone compound in promoting the growth of plant roots, and also provides a plant root growth promoter, which effectively promotes the growth and development process of crops, and achieves the effects of stabilizing yield, increasing production, improving quality and the like.
The application of hydroxyketone compounds or preparations containing the hydroxyketone compounds in promoting the growth of plant roots, wherein the hydroxyketone compounds are at least one of compounds shown in a formula I and a formula II,
the preparation containing the hydroxyketone compound takes the hydroxyketone compound as an active component and also comprises auxiliary materials. The above hydroxyketone compound can be used alone or in combination with other adjuvants in the growth process of plant roots.
The hydroxyketone compound or the preparation containing the hydroxyketone compound has at least one of the following functions in promoting the growth of plant seedlings:
promoting the growth of the roots of the plant seedlings;
promoting the increase of the weight of the roots of the plant seedlings.
The hydroxy ketone compound is composed of compounds shown in a formula I and a formula II, and further is composed of compounds shown in the formula I and the formula II according to the mass ratio of 1: 10-10: 1.
Furthermore, the hydroxyketone compound is composed of compounds shown in a formula I and a formula II according to a mass ratio of 2: 3.
Further, the plant is corn, rice, cucumber or tomato.
The hydroxyketone compounds shown in formula I and formula II are prepared from fermentation cultures of bacillus WRN 031.
The Bacillus subtilis WRN031 of the method can be obtained by an Agricultural microbial strain preservation management center (ACCC) of China, with a preservation number ACCC 19993; and can be purchased commercially by agricultural resources and agricultural district research institutes of Chinese agricultural academy of sciences.
Further preferably, the hydroxyketones of formula I and formula II are prepared as follows: the bacillus subtilis is obtained by extracting fermentation culture solution of bacillus with ethyl acetate and then separating the ethyl acetate extract through silica gel column chromatography, wherein the silica gel column chromatography adopts petroleum ether and acetone as elution conditions, and the acetone is 2: 1.
Another object of the present invention is to provide a plant root growth promoter.
A plant root growth promoter comprises active component of hydroxyketone compound, wherein the hydroxyketone compound is at least one of compounds shown in formula I and formula II,
further, the promoter has at least one of the following functions:
promoting the growth of the roots of the plant seedlings;
promoting the increase of the weight of the roots of the plant seedlings.
Further, the active ingredient of the accelerant is composed of compounds shown in a formula I and a formula II according to the mass ratio of 1: 10-10: 1.
Furthermore, the active component of the accelerator consists of compounds shown in a formula I and a formula II according to a mass ratio of 2: 3.
The accelerator disclosed by the invention preferably consists of a hydroxyketone compound and auxiliary materials.
Further, the accelerant consists of the following components in percentage by mass: 0.5-15% of hydroxyl ketone compound, 0.2-2% of cosolvent, 0.1-3% of preservative, 1-10% of auxiliary material assistant and the balance of water.
Wherein the cosolvent is one or two of sodium benzoate, sodium salicylate, p-aminobenzoic acid, tween 20 and tween 80; the preservative is one or more of benzoic acid, sorbic acid, dehydroacetic acid, sodium diacetate, calcium acetate, calcium propionate, phosphate, ethyl p-hydroxybenzoate and propyl p-hydroxybenzoate; the auxiliary material auxiliary agent is one or more of alkylphenol polyoxyethylene, alkylbenzene sulfonate, naphthalene sulfonate, a sulfonate formaldehyde condensate, castor oil polyoxyethylene polyoxypropylene ether, fatty acid polyoxyethylene ester phosphate and alkyl polyoxyethylene ether phosphate.
It is another object of the present invention to provide a method for promoting the growth of young plants.
A method of promoting the growth of young plants by applying the plant root promoter to plant seeds or young plants.
The active component of the accelerant is a hydroxyketone compound, the hydroxyketone compound is at least one of compounds with a formula I and a formula II,
furthermore, the application amount of the seedling growth promoter is 0.04-16 mu g per plant in terms of hydroxyl ketone compounds.
Furthermore, the application amount of the seedling growth promoter is 0.08-16 mu g per plant based on the hydroxyketone compound.
The activity test shows that the plant root growth promoter of the invention has obvious promotion effect on the roots of corn, rice, cucumber and tomato. In practical use, the composition of the plant root growth promoting compounds I and II of the present invention is applied to the target plants in the following amounts: the amount ranges from 0.04 to 16 mu g per strain, and the combined mass ratio of the compound I to the compound II is 1:10 to 10: 1; the plant root growth promoter of the present invention, the compound I or II monomer, is applied to the target plant in the following amount: the amount is in the range of 0.04-16 mu g per strain. The plant root growth promoter of the invention can act on the roots of plants, thereby achieving the effects of increasing the yield, improving the quality and the like.
The invention has the beneficial effects that: the method is simple and low in cost, and the plant root growth promoter can obviously promote the growth of plant roots and improve the growth capacity of plants in the seedling stage, thereby achieving the purpose of increasing yield.
Detailed Description
The following non-limiting examples are presented to enable those of ordinary skill in the art to more fully understand the present invention and are not intended to limit the invention in any way.
The test methods described in the following examples are all conventional methods unless otherwise specified; the reagents and materials are commercially available, unless otherwise specified.
Isolation and characterization of Compounds of formula I and formula II (hereinafter referred to simply as Compound I and Compound II) used in the examples described below
Bacillus WRN031 (commercially available from institute of agricultural resources and agricultural divisions of Chinese academy of agricultural sciences) was cultured in Landy medium and fermented at 37 deg.C for 36h to obtain 100L fermentation broth. And extracting the fermentation liquor by using ethyl acetate to obtain 80g of ethyl acetate extract. The ethyl acetate extract was separated by silica gel column chromatography, eluting with petroleum ether and acetone 2:1 to give compound I8g (> 95% pure) and compound II12g (> 93% pure), respectively.
The 1D NMR data for compounds I and II are as follows:
a compound I:1H-NMR(600MHz,CDCl3),δ2.23(3H,s,H-1),1.42(3H,s,3-CH3),3.85(1H,q,J=6.4Hz,H-4),1.06(3H,d,J=6.4Hz,H-5);13C-NMR(150MHz,CDCl3),δ211.0(s,C-2),81.4(s,C-3),71.2(d,C-4),25.0(q,C-5),21.8(q,C-1),17.9(q,3-CH3)。
compound II:1H-NMR(600MHz,CDCl3),δ2.29(3H,s,H-1),1.26(3H,s,3-CH3),4.03(1H,q,J=6.3Hz,H-4),1.28(3H,d,J=6.3Hz,H-5);13C-NMR(150MHz,CDCl3),δ211.5(s,C-2),81.4(s,C-3),71.0(d,C-4),23.8(q,C-5),21.5(q,C-1),16.9(q,3-CH3)。
example 1 promoting activity of different amounts of compound I and II compositions (compound I: II ═ 2:3, m/m) on corn root growth
The compositions of compounds I and II (compound I: II ═ 2:3, m/m) were tested for their corn root growth promoting activity by the sand culture method at different application rates. The method comprises the steps of firstly sterilizing sand for 3 times continuously under the condition of 30min by using a high-temperature sterilizing pot, sterilizing corn seeds (84 sterilizing liquid: water is 3:7) for 10-12min, then cleaning the corn seeds for 4-5 times by using sterile water, planting the corn seeds into pots containing about 700g of sand (sterilization), culturing the corn seeds in a culture room at 28 ℃ in 5 seeds in each pot, applying different amounts of compound I and compound II compositions (2:3, m/m) to germinated corn plants after the plants germinate, wherein the application amount of the compound I and compound II compositions is 0, 0.04, 0.08, 0.4, 0.8, 4, 8 and 16 mu g/plant, three times are set for each application amount, and 0 mu g/plant is a control group (CK). The root length of the corn was measured on day 7.
The results show that the compositions of compounds I and II (2:3, m/m) applied at different amounts have growth promoting effect on the roots of corn and show a certain concentration increase dependence.
TABLE 1 corn root promoting Activity of different application amounts of Compound I and II compositions (2:3, m/m)
Application amount | Root length (cm) | SD(cm) |
0 μ g/strain (CK) | 13.86 | 0.45 |
0.04 μ g/strain | 14.03 | 1.11 |
0.08. mu.g/strain | 13.92 | 0.78 |
0.4. mu.g/strain | 15.67 | 1.28 |
0.8. mu.g/strain | 16.61 | 2.32 |
4 μ g/strain | 19.43 | 1.57 |
8 mu g/strain | 18.64 | 0.97 |
16 μ g/strain | 17.95 | 0.72 |
EXAMPLE 2 corn root growth promoting Activity of different ratio combinations of Compounds I and II
The sand culture method described in example 1 was used to test the growth promoting activity of corn roots by using compositions of compounds I and II (compound I: II: 1: 10-10: 1) in different proportions, and the application amount of the composition was 4 μ g/strain. As a result, it was found that all the ratios promoted the growth of corn roots, but the root growth promoting activity of compound I: II ═ 2:3 was better.
TABLE 2 growth promoting Activity of different proportions of the compositions of Compounds I and II on corn roots (application amount 4. mu.g/strain)
EXAMPLE 3 growth promoting Activity of Compounds I and II monomers on corn root
The compounds I and II were each applied in the gradient described in example 1, and the growth-promoting effect of the individual compounds on the corn roots was determined by the sand culture method in example 1 at different application rates. The results show that the monomers of the compounds I and II have obvious promotion effect on the roots of the corn.
TABLE 3 corn root promoting Activity of Compound I
Application amount | Root length (cm) | SD(cm) |
0 μ g/strain (CK) | 14.76 | 0.78 |
0.08. mu.g/strain | 15.66 | 0.43 |
0.4. mu.g/strain | 16.82 | 1.76 |
0.8. mu.g/strain | 17.83 | 1.21 |
4 μ g/strain | 18.33 | 1.57 |
8 mu g/strain | 18.41 | 1.45 |
TABLE 4 corn root promoting Activity of Compound II
Application amount | Root length (cm) | SD(cm) |
0 μ g/strain (CK) | 12.61 | 2.72 |
0.08. mu.g/strain | 13.63 | 2.66 |
0.4. mu.g/strain | 14.54 | 1.82 |
0.8. mu.g/strain | 15.35 | 2.75 |
4 μ g/strain | 17.65 | 3.69 |
8 mu g/strain | 18.34 | 1.68 |
EXAMPLE 4 promoting Activity of different amounts of Compound I and II compositions (2:3, m/m) on cucumber root growth
According to the experimental method of sand culture in example 1, different amounts of a combination of compounds I and II (2:3, m/m) were applied to the roots of cucumber seedlings, and the length of the roots of the cucumbers was measured after 15 days. Test results show that the composition (2:3, m/m) of the compounds I and II has obvious promotion effect on the growth of cucumber roots.
TABLE 5 cucumber root promoting Activity of the combination of Compounds I and II (2:3, m/m)
Application amount | Root length (cm) | SD(cm) |
0 μ g/strain (CK) | 5.02 | 1.23 |
0.08. mu.g/strain | 5.78 | 0.97 |
0.4. mu.g/strain | 5.96 | 1.18 |
0.8. mu.g/strain | 6.45 | 0.54 |
4 μ g/strain | 6.89 | 0.82 |
8 mu g/strain | 7.43 | 1.01 |
EXAMPLE 5 cucumber root growth promoting Activity of Compounds I and II monomers
According to the experimental method of sand culture in example 1, different amounts of monomers of compounds I and II were applied to the roots of cucumber seedlings, and the length of the roots of the cucumber was measured 15 days later. Test results show that the compounds I and II have obvious root growth promoting effect on cucumbers.
TABLE 6 growth promoting Activity of Compound I on cucumber roots
Application amount | Root length (cm) | SD(cm) |
0 μ g/strain (CK) | 4.89 | 0.78 |
0.08. mu.g/strain | 5.23 | 0.43 |
0.4. mu.g/strain | 5.84 | 1.26 |
0.8. mu.g/strain | 6.66 | 0.79 |
4 μ g/strain | 7.01 | 0.76 |
8 mu g/strain | 7.238 | 0.54 |
TABLE 7 growth promoting Activity of Compound II on cucumber roots
Application amount | Root length (cm) | SD(cm) |
0 μ g/strain (CK) | 5.18 | 0.67 |
0.08. mu.g/strain | 5.20 | 0.74 |
0.4. mu.g/strain | 5.82 | 0.32 |
0.8. mu.g/strain | 6.34 | 0.57 |
4 μ g/strain | 6.89 | 0.79 |
8 mu g/strain | 7.69 | 0.41 |
EXAMPLE 6 accelerating Activity of different application amounts of Compound I and II compositions (2:3, m/m) on tomato root growth
According to the experimental method of sand culture in example 1, different amounts of the combination of compounds I and II (2:3, m/m) were applied to the roots of tomato seedlings and the length of the roots of the tomatoes was measured after 15 days. The test results show that the composition of the compounds I and II (2:3, m/m) has obvious promotion effect on the growth of tomato roots.
TABLE 8 growth promoting Activity of compositions of Compounds I and II (2:3, m/m) on tomato roots
Application amount | Root length (cm) | SD(cm) |
0 μ g/strain (CK) | 6.99 | 0.78 |
0.08. mu.g/strain | 7.04 | 0.43 |
0.4μg/strain | 7.65 | 1.06 |
0.8. mu.g/strain | 8.49 | 1.02 |
4 μ g/strain | 8.93 | 0.59 |
8 mu g/strain | 9.84 | 0.45 |
EXAMPLE 7 promoting Activity of Compounds I and II monomers on tomato root growth
According to the experimental method of sand culture in example 1, different amounts of the monomers of compounds I and II were applied to the roots of tomato seedlings, and the length of the roots of the tomatoes was measured after 15 days. The test results show that the composition of the compounds I and II (2:3, m/m) has obvious promotion effect on the growth of tomato roots.
TABLE 9 growth promoting Activity of Compound I on tomato roots
Application amount | Root length (cm) | SD(cm) |
0 μ g/strain (CK) | 7.76 | 0.78 |
0.08. mu.g/strain | 8.03 | 0.44 |
0.4. mu.g/strain | 8.69 | 0.97 |
0.8. mu.g/strain | 9.42 | 1.21 |
4 μ g/strain | 9.89 | 0.57 |
8 mu g/strain | 10.54 | 1.01 |
TABLE 10 growth promoting Activity of Compound II on tomato roots
EXAMPLE 8 growth promoting Activity of different application amounts of Compound I and II compositions (2:3, m/m) on Rice roots
The method adopted is a seed bag culture method, firstly, rice seeds are placed in a refrigerator at 4 ℃ for vernalization for 3d, the vernalized rice seeds (84 disinfectant solution: water: 3:7) are disinfected for 10-12min, then are washed with sterile water for 4-5 times, and the rice seeds are planted in seed bags containing about 30mL of solution, so that the application amount of the compound I and II composition in the seed bags is 0, 0.08, 0.4, 0.8, 4 and 8 mu g/plant, and 0 mu g/plant is used as a control group (CK). 12 seeds were placed in each bag and incubated in a 28 ℃ incubation chamber at triplicate for each concentration, water was used as a control. The growth of the cells was observed for 7 days, and the root length, root weight, overground length and overground weight were measured. Test results show that the composition (2:3, m/m) of the compounds I and II has obvious root growth promoting effect on rice.
TABLE 11 growth promoting Activity of the combination of Compounds I and II (2:3, m/m) on Rice roots
Application amount | Root length (cm) | SD(cm) |
0 μ g/strain (CK) | 15.37 | 0.46 |
0.08. mu.g/strain | 14.04 | 1.14 |
0.4. mu.g/strain | 15.58 | 0.844 |
0.8. mu.g/strain | 17.11 | 0.425 |
4 μ g/strain | 17.76 | 0.488 |
8 mu g/strain | 18.37 | 0.95 |
EXAMPLE 9 Rice root growth promoting Activity of Compounds I and II monomers
The growth-promoting effect of the monomers of the compounds I and II on rice was determined by the seed bag culture method in example 8, and the compositions of the compounds I and II were applied at 0, 0.08, 0.4, 0.8, 4 and 8. mu.g/strain, and 0. mu.g/strain was used as a control group (CK). The length of the roots of rice was measured 7 days later. Test results show that the compounds I and II have obvious root growth promoting effect on rice.
TABLE 12 growth promoting Activity of Compound I on Rice roots
TABLE 13 growth promoting Activity of Compound II on Rice roots
Application amount | Root length (cm) | SD(cm) |
0 μ g/strain (CK) | 13.74 | 0.85 |
0.08. mu.g/strain | 13.39 | 2.13 |
0.4. mu.g/strain | 14.96 | 0.77 |
0.8. mu.g/strain | 15.69 | 0.84 |
4 μ g/strain | 16.13 | 0.48 |
8 mu g/strain | 17.46 | 1.30 |
EXAMPLE 10 preparation of Compound I and II compositions plant growth promoters
The plant growth promoter prepared from the compound I and the compound II comprises the following components in percentage by mass: 0.5-15% of compound I and compound II composition, 0.2-2% of cosolvent, 0.1-3% of preservative, 1-10% of auxiliary material auxiliary agent and the balance of water.
Wherein the cosolvent is one or two of sodium benzoate, sodium salicylate, p-aminobenzoic acid, tween 20 and tween 80; the preservative is one or more of benzoic acid, sorbic acid, dehydroacetic acid, sodium diacetate, calcium acetate, calcium propionate, phosphate, ethyl p-hydroxybenzoate and propyl p-hydroxybenzoate; the auxiliary material auxiliary agent is one or more of alkylphenol polyoxyethylene, alkylbenzene sulfonate, naphthalene sulfonate, a sulfonate formaldehyde condensate, castor oil polyoxyethylene polyoxypropylene ether, fatty acid polyoxyethylene ester phosphate and alkyl polyoxyethylene ether phosphate.
Formula 1: the composition 1:2 of the compounds I and II comprises 1 percent of sodium salicylate, 0.5 percent of sodium benzoate, 1 percent of sodium benzoate, 6 percent of alkylphenol polyoxyethylene and the balance of water.
And (2) formula: 5 percent of compound I and compound II composition 1:5, 1 percent of p-aminobenzoic acid, 0.2 percent of sodium benzoate, 10 percent of alkylphenol polyoxyethylene and the balance of water.
And (3) formula: the composition 2:3 of the compounds I and II is 8%, the Tween 20 is 2%, the dehydroacetic acid is 1%, the alkyl polyoxyethylene ether phosphate ester is 3%, and the balance of water.
And (4) formula: compound I and II composition 3:2 was 8%, tween 80 was 1%, calcium acetate 0.7%, sulfonate formaldehyde condensate, balance water.
And (5) formula: composition 5:1 of compound I and II is 10%, ethyl p-hydroxybenzoate 1%, sodium benzoate 0.5%, alkylbenzene sulfonate 6%, and balance water.
And (6) formula: compound I and II composition 4:3 was 15%, propyl paraben 0.5%, tween 80 was 1%, castor oil polyoxyethylene polyoxypropylene ether 8%, and balance water.
Example 11 application of the plant growth promoter of the compound I and II compositions in the aspect of promoting the growth of rice seedlings the plant growth promoter of the compound I and II compositions was obtained according to the formulations 1 to 6 in example 10. Germinating and growing seedlings of Pioneer No. 8 rice, transplanting the seedlings to a test field, spraying the plant growth promoters of the compound I and compound II compositions of the formula 1-6 according to a dose of 20 g/mu (about 1mg of promoter per plant), setting a control (not spraying), and after spraying for 10 days, selecting 10 seedlings to calculate the root length (centimeter, cm).
TABLE 14 plant growth promoting Activity of Compound I and II compositions on Rice seedlings in test fields
Compound I and II composition formula | Root length (cm) | SD(cm) |
Control | 12.56 | 1.23 |
Formulation 1 | 15.92 | 0.89 |
Formulation 2 | 14.38 | 2.35 |
Formulation 3 | 17.26 | 1.01 |
Formulation 4 | 16.37 | 1.32 |
Formulation 5 | 18.55 | 0.56 |
EXAMPLE 12 monomeric plant growth promoter preparation of Compounds I and II
The plant growth promoter prepared from the monomers of the compounds I and II comprises the following components in percentage by mass: 0.5-15% of monomers of the compounds I and II, 0.2-2% of cosolvent, 0.1-3% of preservative, 1-10% of auxiliary material auxiliary agent and the balance of water.
Wherein the cosolvent is one or two of sodium benzoate, sodium salicylate, p-aminobenzoic acid, tween 20 and tween 80; the preservative is one or more of benzoic acid, sorbic acid, dehydroacetic acid, sodium diacetate, calcium acetate, calcium propionate, phosphate, ethyl p-hydroxybenzoate and propyl p-hydroxybenzoate; the auxiliary material auxiliary agent is one or more of alkylphenol polyoxyethylene, alkylbenzene sulfonate, naphthalene sulfonate, a sulfonate formaldehyde condensate, castor oil polyoxyethylene polyoxypropylene ether, fatty acid polyoxyethylene ester phosphate and alkyl polyoxyethylene ether phosphate.
Formula 1: 0.5% of monomer of compound I, 2% of sodium salicylate, 2% of sodium benzoate, 4% of alkylphenol polyoxyethylene and the balance of water.
And (2) formula: 2% of monomer of compound I, 0.5% of p-aminobenzoic acid, 0.2% of sorbic acid, 5% of alkylbenzene sulfonate and the balance of water.
And (3) formula: 5% of monomer of compound I, 2% of Tween 20, 1% of dehydroacetic acid, 5% of naphthalene sulfonate and the balance of water.
And (4) formula: 8% of monomer of compound I, 2% of Tween 80, 1% of calcium propionate, 10% of castor oil polyoxyethylene polyoxypropylene ether and the balance of water.
And (5) formula: 10% of monomer of compound I, 1% of Tween 20, 0.5% of sodium benzoate, 2% of propyl p-hydroxybenzoate, 3% of fatty acid polyoxyethylene ester phosphate and the balance of water.
And (6) formula: 1% of monomer of compound II, 1% of Tween 80, 0.5% of propyl p-hydroxybenzoate, 8% of alkylphenol polyoxyethylene and the balance of water.
And (3) formula 7: 3% of monomer of compound II, 1% of sodium salicylate, 0.5% of ethyl p-hydroxybenzoate, 2% of castor oil polyoxyethylene polyoxypropylene ether and the balance of water.
And (4) formula 8: 5% of monomer of compound II, 0.5% of sodium benzoate, 1% of sorbic acid, 2% of fatty acid polyoxyethylene ester phosphate and the balance of water.
Formula 9: 10% of monomer of compound II, 1% of Tween 80, 2% of sodium salicylate, 0.5% of propyl p-hydroxybenzoate, 8% of castor oil polyoxyethylene polyoxypropylene ether and the balance of water.
Formula 10: 15% of monomer of compound II, 1% of Tween 20, 0.2% of propyl p-hydroxybenzoate, 1% of calcium propionate, 5% of alkylbenzene sulfonate and the balance of water.
EXAMPLE 13 use of monomeric plant growth promoters of Compounds I and II in the promotion of Rice seedling growth
The monomeric plant growth promoter of the compound I and II is obtained according to the formula 1-10 in the embodiment 12. Germinating and growing seedlings of Pioneer No. 8 rice, transplanting the seedlings to a test field, spraying the compound I and II monomer plant growth promoters with the formulas 1-6 according to the dose of 20 g/mu (about 1mg promoter/plant), setting a control (not spraying), and after spraying for 10 days, selecting 10 seedlings to calculate the root length (centimeter, cm).
TABLE 15 promoting Activity of monomeric plant growth promoters of Compounds I and II on Rice seedlings in test fields
From the above experimental results, it can be seen that the compounds I and II of the present invention can significantly increase the root length of plants. The above embodiments are merely illustrative, and not restrictive, and those skilled in the art can make various changes and modifications without departing from the spirit and scope of the invention. Therefore, all equivalent technical solutions also belong to the scope of the present invention, and the protection scope of the present invention should be defined by the claims.
Claims (7)
2. use according to claim 1, characterized in that: the hydroxyketone compound is composed of compounds shown in a formula I and a formula II according to a mass ratio of 1: 10-10: 1.
3. Use according to claim 1, characterized in that: the plant is corn, rice, cucumber or tomato.
4. Use according to claim 1, characterized in that: the hydroxyketone compound is prepared from a fermentation culture of bacillus.
5. Use according to claim 1, characterized in that: the preparation containing the hydroxyl ketone compound comprises the following components in percentage by mass: 0.5-15% of hydroxyl ketone compound, 0.2-2% of cosolvent, 0.1-3% of preservative, 1-10% of auxiliary material auxiliary agent and the balance of water,
wherein the cosolvent is one or two of sodium benzoate, sodium salicylate, p-aminobenzoic acid, tween 20 and tween 80; the preservative is one or more of benzoic acid, sorbic acid, dehydroacetic acid, sodium diacetate, calcium acetate, calcium propionate, phosphate, ethyl p-hydroxybenzoate and propyl p-hydroxybenzoate; the auxiliary material auxiliary agent is one or more of alkylphenol polyoxyethylene, alkylbenzene sulfonate, naphthalene sulfonate, a sulfonate formaldehyde condensate, castor oil polyoxyethylene polyoxypropylene ether, fatty acid polyoxyethylene ester phosphate and alkyl polyoxyethylene ether phosphate.
6. Use according to claim 1 or 5, characterized in that: applying a hydroxyketone compound or a preparation containing a hydroxyketone compound to a plant seed or a plant seedling.
7. Use according to claim 6, characterized in that: the application amount of the hydroxyketone compound or the preparation containing the hydroxyketone compound is 0.04-16 mu g/strain based on the hydroxyketone compound.
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CN110041166A (en) * | 2019-05-09 | 2019-07-23 | 沈阳农业大学 | A kind of Dracaena cochinchinensis extract and its preparation and application with bacteria resistance function |
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CN110041166A (en) * | 2019-05-09 | 2019-07-23 | 沈阳农业大学 | A kind of Dracaena cochinchinensis extract and its preparation and application with bacteria resistance function |
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