CN110463603A - A kind of succulent quick breeding by group culture method based on explant - Google Patents
A kind of succulent quick breeding by group culture method based on explant Download PDFInfo
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- CN110463603A CN110463603A CN201810436542.1A CN201810436542A CN110463603A CN 110463603 A CN110463603 A CN 110463603A CN 201810436542 A CN201810436542 A CN 201810436542A CN 110463603 A CN110463603 A CN 110463603A
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- explant
- succulent
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- multiple buds
- vitamin
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
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- Botany (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The succulent quick breeding by group culture method based on explant that the invention discloses a kind of is that three steps of being taken root by the selection of explant and disinfection, the induction of Multiple Buds and Multiple Buds carry out culture and obtain tissue-cultured seedling, it includes selection and disinfection of 1) explant；2) induction of Multiple Buds；3) Multiple Buds are taken root.Succulent quick breeding by group culture method of the invention carries out culture and obtains tissue-cultured seedling using the blade of succulent as explant by the selection of explant and disinfection, the induction of Multiple Buds and Multiple Buds three steps of taking root.This method material is easy to get, disinfection is convenient, high survival rate, it is with short production cycle, improve the breeding coefficient and reproduction speed of succulent, its germination rate and rooting rate are 3 times of conventional medium or so, and can preferably keep the strain advantage of succulent, can quickly breed a large amount of tissue-cultured seedling for being suitble to transplanting.
Description
Technical field
The present invention relates to plant tissue culture technical fields, quick more particularly to a kind of succulent tissue culture based on explant
Propagation method.
Background technique
Succulent is also known as succulent, succulent plant, on gardening be sometimes referred to as fleshiness flowers, but with succulent this
Title is the most commonly used.Succulent refers to certain a part of vegetable nutritorium, as stem or leaf or root have flourishing thin-walled group
Knit a kind of plant for the plump succulence that seems in shape to store moisture.In recent years, the market demand pole of succulent
Greatly, there is the phenomenon that supply falls short of demand, however the development of China succulent is later, especially on succulent tissue cultures.When
Before, although the tissue cultures for having had Some Enterprises to carry out succulent, the low reproduction rate of culture, rooting rate are low,
Deficiency in economic performance, therefore establish a kind of efficient succulent quick breeding by group culture method and have great importance.
Summary of the invention
The succulent quick breeding by group culture method based on explant that the object of the present invention is to provide a kind of, to improve fleshiness
The breeding coefficient and reproductive efficiency of plant.
The scheme that the present invention solves technical problem is: a kind of succulent quick breeding by group culture method based on explant,
It is that three steps of being taken root by the selection of explant and disinfection, the induction of Multiple Buds and Multiple Buds carry out culture and obtain tissue-cultured seedling,
It comprising the following specific steps
1) explant select and disinfection: using the blade of succulent as explant, blade is cleaned with distilled water, sterile water rinse
3~5 times, blade is placed in the mercuric chloride solution that mass fraction is 0.03~0.06% and impregnates 1~2min, with sterile water rinse 3
~5 times, blade is placed in the liquor natrii hypochloritis that mass fraction is 2~4% and impregnates 2~4min, with sterile water rinse 3~5
It is secondary, blade is placed at 40~45 DEG C and carries out 30~45min of heat treatment, blade is placed under 0.3~0.5MPa atmospheric pressure and handles 3
After~5min, blade is placed in the mercuric chloride solution that mass fraction is 0.03~0.06% and impregnates 1~2min, is moistened with sterile water
Wash 3~5 times, by blade be placed in mass fraction be 2~4% liquor natrii hypochloritis in impregnate 2~4min, with sterile water rinse 3~
5 times, obtain aseptic explant；
2) induction of Multiple Buds: the aseptic explant that step 1) is obtained is cut into pieces to be inoculated in adventitious shoots culture base and be cultivated,
Condition of culture are as follows: 28~31 DEG C of temperature, air humidity 55~65%, intensity of illumination be 1200~1400lux, light application time 15~
17h/d, incubation time is 28~32d, to obtain Multiple Buds；
3) Multiple Buds are taken root: the Multiple Buds that step 2 is obtained are transferred culture of rootage in root media, condition of culture are as follows: temperature
30~32 DEG C of degree, air humidity 55~65%, intensity of illumination is 1200~1400lux, 12~14h/d of light application time, when culture
Between be 35~40d, to obtain complete plant.
The root media component are as follows: by mass percentage, sucrose 3~4%, MS+6-BA0.16~0.18%,
NAA0.10~0.12%, IAA0.02~0.03%, vitamin B1 0.004~0.006%, vitamin B2 0.003~0.005%,
Vitamin B6 0.001~0.003%, Vitamin B9 0.002~0.004%, surplus are water.
In step 2, the adventitious shoots culture base component are as follows: by mass percentage, sucrose 3~4%, antibiotic 0.01
~0.03%, zinc chloride 0.01~0.02%, MS+6-BA0.2~0.4%, NAA0.2~0.3%, IAA0.08~0.10%, vitamin
B1 0.015~0.025%, vitamin B2 0.008~0.010%, vitamin B6 0.006~0.008%, Vitamin B9 0.01
~0.03%, surplus is water.
The invention has the advantages that: succulent quick breeding by group culture method of the invention, with succulent
Blade is explant, is cultivated by selection and the disinfection of explant, the induction of Multiple Buds and Multiple Buds three steps of taking root
Obtain tissue-cultured seedling.This method material is easy to get, and disinfection is convenient, and high survival rate is with short production cycle, improves the line of breeding of succulent
Several and reproduction speed, germination rate and rooting rate are 3 times of conventional medium or so, and can preferably keep the product of succulent
It is advantage, can quickly breeds a large amount of tissue-cultured seedling for being suitble to transplanting, meet production requirement, improve economic benefit.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail.
Embodiment
Succulent quick breeding by group culture method of the invention is lured by the selection of explant and disinfection, Multiple Buds
Lead with Multiple Buds take root three steps carry out culture obtain tissue-cultured seedling, it comprising the following specific steps
1) explant select and disinfection: using the blade of succulent as explant, blade is cleaned with distilled water, sterile water rinse
4 times, blade is placed in the mercuric chloride solution that mass fraction is 0.05% and impregnates 2min, with sterile water rinse 4 times, blade is set
3min is impregnated in the liquor natrii hypochloritis that mass fraction is 3%, with sterile water rinse 4 times, blade is placed at 45 DEG C and carries out heat
45min is handled, blade is placed under 0.5MPa atmospheric pressure after handling 4min, blade is placed in the chlorination that mass fraction is 0.05%
2min is impregnated in mercury solution, with sterile water rinse 4 times, blade is placed in the liquor natrii hypochloritis that mass fraction is 3% and is impregnated
4min obtains aseptic explant with sterile water rinse 4 times；
2) induction of Multiple Buds: the aseptic explant that step 1) is obtained is cut into pieces to be inoculated in adventitious shoots culture base and be cultivated,
Condition of culture are as follows: 30 DEG C of temperature, air humidity 65%, intensity of illumination 1400lux, light application time 16h/d, incubation time is
30d, to obtain Multiple Buds；
3) Multiple Buds are taken root: the Multiple Buds that step 2 is obtained are transferred culture of rootage in root media, condition of culture are as follows: temperature
32 DEG C of degree, air humidity 65%, intensity of illumination 1400lux, light application time 14h/d, incubation time 40d are complete to obtain
Plant.
The root media component are as follows: by mass percentage, sucrose 4%, MS+6-BA0.16%, NAA0.10%,
IAA0.02%, vitamin B1 0.005%, vitamin B2 0.005%, vitamin B6 0.001%, Vitamin B9 0.002%, surplus
For water.
In step 2, the adventitious shoots culture base component are as follows: by mass percentage, sucrose 4%, antibiotic 0.03%,
Zinc chloride 0.02%, MS+6-BA0.2%, NAA0.2%, IAA0.08%, vitamin B1 0.015%, vitamin B2 0.008%, dimension life
Plain B6 0.006%, Vitamin B9 0.01%, surplus are water.
Succulent quick breeding by group culture method of the invention passes through explant using the blade of succulent as explant
Selection and disinfection, the induction of Multiple Buds and Multiple Buds take root three steps carry out culture obtain tissue-cultured seedling.This method material is easy
, disinfection is convenient, and high survival rate is with short production cycle, improve the breeding coefficient and reproduction speed of succulent, germination rate and
Rooting rate is 3 times of conventional medium or so, and can preferably keep the strain advantage of succulent, can quickly be bred a large amount of
It is suitble to the tissue-cultured seedling of transplanting.
Claims (3)
1. a kind of succulent quick breeding by group culture method based on explant, it is characterised in that: be the selection by explant
Carry out culture with disinfection, the induction of Multiple Buds and Multiple Buds three steps of taking root and obtain tissue-cultured seedling, it comprising the following specific steps
1) explant select and disinfection: using the blade of succulent as explant, blade is cleaned with distilled water, sterile water rinse
3~5 times, blade is placed in the mercuric chloride solution that mass fraction is 0.03~0.06% and impregnates 1~2min, with sterile water rinse 3
~5 times, blade is placed in the liquor natrii hypochloritis that mass fraction is 2~4% and impregnates 2~4min, with sterile water rinse 3~5
It is secondary, blade is placed at 40~45 DEG C and carries out 30~45min of heat treatment, blade is placed under 0.3~0.5MPa atmospheric pressure and handles 3
After~5min, blade is placed in the mercuric chloride solution that mass fraction is 0.03~0.06% and impregnates 1~2min, is moistened with sterile water
Wash 3~5 times, by blade be placed in mass fraction be 2~4% liquor natrii hypochloritis in impregnate 2~4min, with sterile water rinse 3~
5 times, obtain aseptic explant；
2) induction of Multiple Buds: the aseptic explant that step 1) is obtained is cut into pieces to be inoculated in adventitious shoots culture base and be cultivated,
Condition of culture are as follows: 28~31 DEG C of temperature, air humidity 55~65%, intensity of illumination be 1200~1400lux, light application time 15~
17h/d, incubation time is 28~32d, to obtain Multiple Buds；
3) Multiple Buds are taken root: the Multiple Buds that step 2 is obtained are transferred culture of rootage in root media, condition of culture are as follows: temperature
30~32 DEG C of degree, air humidity 55~65%, intensity of illumination is 1200~1400lux, 12~14h/d of light application time, when culture
Between be 35~40d, to obtain complete plant.
2. a kind of succulent quick breeding by group culture method based on explant according to claim 1, it is characterised in that:
The root media component are as follows: by mass percentage, sucrose 3~4%, MS+6-BA0.16~0.18%, NAA0.10~
0.12%, IAA0.02~0.03%, vitamin B1 0.004~0.006%, vitamin B2 0.003~0.005%, vitamin B6
0.001~0.003%, Vitamin B9 0.002~0.004%, surplus are water.
3. a kind of succulent quick breeding by group culture method based on explant according to claim 1, it is characterised in that:
In step 2, the adventitious shoots culture base component are as follows: by mass percentage, sucrose 3~4%, antibiotic 0.01~0.03%,
Zinc chloride 0.01~0.02%, MS+6-BA0.2~0.4%, NAA0.2~0.3%, IAA0.08~0.10%, vitamin B1 0.015
~0.025%, vitamin B2 0.008~0.010%, vitamin B6 0.006~0.008%, Vitamin B9 0.01~0.03%,
Surplus is water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810436542.1A CN110463603A (en) | 2018-05-09 | 2018-05-09 | A kind of succulent quick breeding by group culture method based on explant |
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|---|---|---|---|
| CN201810436542.1A CN110463603A (en) | 2018-05-09 | 2018-05-09 | A kind of succulent quick breeding by group culture method based on explant |
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| CN201810436542.1A Pending CN110463603A (en) | 2018-05-09 | 2018-05-09 | A kind of succulent quick breeding by group culture method based on explant |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112400696A (en) * | 2020-12-28 | 2021-02-26 | 内蒙古蒙草生态环境（集团）股份有限公司 | Tissue culture method of evergreen common selfheal fruit-spike bamboo |
| CN112400695A (en) * | 2020-12-28 | 2021-02-26 | 内蒙古蒙草生态环境（集团）股份有限公司 | Culture medium for culturing evergreen common summer pink |
| CN116098064A (en) * | 2023-03-15 | 2023-05-12 | 湖南文理学院 | General open tissue culture method for Crassulaceae succulent plants |
-
2018
- 2018-05-09 CN CN201810436542.1A patent/CN110463603A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112400696A (en) * | 2020-12-28 | 2021-02-26 | 内蒙古蒙草生态环境（集团）股份有限公司 | Tissue culture method of evergreen common selfheal fruit-spike bamboo |
| CN112400695A (en) * | 2020-12-28 | 2021-02-26 | 内蒙古蒙草生态环境（集团）股份有限公司 | Culture medium for culturing evergreen common summer pink |
| CN116098064A (en) * | 2023-03-15 | 2023-05-12 | 湖南文理学院 | General open tissue culture method for Crassulaceae succulent plants |
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Application publication date: 20191119 |
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