CN110456033A - For adjusting the density of fluid sample containing cell and/or the system of volume - Google Patents
For adjusting the density of fluid sample containing cell and/or the system of volume Download PDFInfo
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- CN110456033A CN110456033A CN201910594172.9A CN201910594172A CN110456033A CN 110456033 A CN110456033 A CN 110456033A CN 201910594172 A CN201910594172 A CN 201910594172A CN 110456033 A CN110456033 A CN 110456033A
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- centrifugal treating
- dilution
- bag
- collection device
- fluid sample
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
Abstract
A kind of system of density and/or volume for adjusting fluid sample containing cell provided herein, the system comprises: sample sack；Waste liquid collecting bag；Dilution bag；Collection device；The hollow centrifugal treating room that can be rotated around rotary shaft；For connecting the pipe-line system of the bag and the centrifugal treating room；And multiple weighing sensors, wherein the system of the density and/or volume for adjusting fluid sample containing cell constitutes closed gnotobasis.
Description
Invention field
The present invention relates to cell sample process fields, and in particular, to one kind is for adjusting fluid sample containing cell
Density and/or volume system and its application method.
Background technique
In recent years, the various biology for being related to cell including cellular immunotherapy and medical application rapid development,
In such applications, it inevitably involves to the volume of sample containing cell (mainly fluid sample) and therein thin
The operation that born of the same parents' density is adjusted.Currently, the operation that the volume and density to sample containing cell are adjusted is mainly hand
Dynamic completion, and also a kind of cell sample can still be handled with while be provided with not without a kind of system or equipment
Multiple cell samples of same volume and different densities.
Therefore, one kind is needed in this field to conveniently realize fluid sample containing cell with high the degree of automation
Volume and/or density adjustment system or equipment and corresponding application method.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of for adjusting the density of fluid sample containing cell
And/or the system of volume.System of the invention can easily realize liquid-like containing cell with higher the degree of automation
The adjustment of the density and/or volume of product, and particularly, by the way that system of the invention is used only, so that it may which easily realizing will
Sample with designated volume and cell density is distributed into the portion with identical or different volume and/or identical or different density
Or the operation of multiple sample.
In one aspect, the present invention provides the following terms:
1. for adjusting the density of fluid sample containing cell and/or the system of volume, the system comprises:
Sample sack；
Waste liquid collecting bag；
Dilution bag；
Collection device, the collection device include one or more collecting bags；
The hollow centrifugal treating room that can be rotated around rotary shaft, accommodate in the centrifugal treating room at the centrifugation
The inner wall cooperation of reason room is axially movable to change the volume of the processing space of the centrifugal treating room to make liquid enter
Or the movable part (preferably piston) of the centrifugal treating room is left, the top of the centrifugal treating room has for allowing liquid
The inlet/outlet of inflow/outflow；
For connecting the pipe-line system of the bag and the centrifugal treating room, the pipe-line system include multiple triple valves or
The combination of corresponding threeway and pipe clamp, wherein being arranged between the sample sack, the waste liquid collecting bag and the centrifugal treating room
There are the combination of the first triple valve or corresponding threeway and pipe clamp, and the centrifugal treating room, the dilution bag and the receipts
The combination of the second triple valve or corresponding threeway and pipe clamp is provided between acquisition means；And
Multiple weighing sensors, the weighing sensor for monitor the sample sack, dilution bag, collecting bag, and/or
Waste liquid collecting bag is because of the weight change caused by being discharged or receiving liquid；
Wherein the system of the density and/or volume for adjusting fluid sample containing cell is constituted closed sterile
Environment.
2. the system according to the 1st, wherein the dilution bag and the collection device be arranged such that it is described
Dilution in dilution bag can be entered in the collection device by gravity, or the dilution is realized by peristaltic pump
Input of the dilution into the collection device in liquid bag, and optionally, the sample sack and the collection device are by cloth
Be set to and the fluid sample in the sample sack entered in the collection device by gravity, or by peristaltic pump come
Realize input of the fluid sample into the collection device in the sample sack.
3. the system according to the 1st, wherein the collection device includes multiple collecting bags in parallel, and by with
Inflow of the combination control liquid of the triple valve of collecting bag number corresponding number or corresponding threeway and pipe clamp to each collecting bag.
4. the system according to the 3rd, wherein the multiple collecting bag is connect with one or more weighing sensors, and
And from a cost perspective, it is preferable that the multiple collecting bag is connect with a weighing sensor.
5. for adjusting the density of fluid sample containing cell and/or the system of volume, the system comprises:
Sample sack；
Waste liquid collecting bag；
Dilution bag；
Collection device, the collection device include multiple collecting bags in parallel；
The hollow centrifugal treating room that can be rotated around rotary shaft, accommodate in the centrifugal treating room at the centrifugation
The inner wall cooperation of reason room is axially movable to change the volume of the processing space of the centrifugal treating room to make liquid enter
Or the movable part (preferably piston) of the centrifugal treating room is left, the top of the centrifugal treating room has for allowing liquid
The inlet/outlet of inflow/outflow；
For connecting the pipe-line system of the bag and the centrifugal treating room, the pipe-line system include multiple triple valves or
The combination of corresponding threeway and pipe clamp, wherein being arranged between the sample sack, the waste liquid collecting bag and the centrifugal treating room
There is the first triple valve, and is provided with the second threeway between the centrifugal treating room, the dilution bag and the collection device
Valve；And
Multiple weighing sensors, the weighing sensor for monitor the sample sack, dilution bag, collecting bag, and/or
Waste liquid collecting bag is because of the weight change caused by being discharged or receiving liquid；
Wherein the system of the density and/or volume for adjusting fluid sample containing cell is constituted closed sterile
Environment, the dilution bag and the collection device are arranged such that the dilution in the dilution bag can pass through gravity
Into in the collection device, or realize by peristaltic pump dilution in the dilution bag into the collection device
Input, and optionally, the sample sack and the collection device are arranged such that the fluid sample in the sample sack
It can be entered in the collection device by gravity, or realize by peristaltic pump fluid sample in the sample sack to institute
The input in collection device is stated, inflow of the liquid to each collecting bag, and the multiple collecting bag and one are controlled by pipe clamp
Weighing sensor connection.
6. using the density of system call interception fluid sample containing cell according to any one of 1-5 and/or
The method of volume, which comprises
A) moving down by the indoor movable part of the centrifugal treating makes the liquid containing cell in sample sack
Sample enters in the centrifugal treating room；
B) by the rotating centrifugal of the centrifugal treating room, keep the cell in the indoor fluid sample of the centrifugal treating heavy
Product is on the inner wall of the centrifugal treating room；
Clear liquid after the centrifugation of specific quantity is discharged by c) moving up by the indoor movable part of the centrifugal treating
Into waste liquid collecting bag；
D) it is rotated by the acceleration or deceleration clockwise or counterclockwise of the centrifugal treating room, makes to be deposited on the centrifugation
Cell in processing chamber interior walls is resuspended in remaining clear liquid；
Cell re-suspension liquid is output to one by e) moving up by the indoor movable part of the centrifugal treating
Or in multiple collecting bags；And optionally
F) dilution of specific quantity is input in each collecting bag from dilution bag,
The amount of the cell re-suspension liquid wherein inputted into multiple collecting bags is same or different, and to multiple collections
The amount of the dilution inputted in bag is same or different.
7. using the density of system call interception fluid sample containing cell according to any one of 1-5 and/or
The method of volume, which comprises
A) moving down by the indoor movable part of the centrifugal treating makes the liquid containing cell in sample sack
Sample enters in the centrifugal treating room；
B) by the rotating centrifugal of the centrifugal treating room, keep the cell in the indoor fluid sample of the centrifugal treating heavy
Product is on the inner wall of the centrifugal treating room；
Whole clear liquids after centrifugation are drained into useless by c) moving up by the indoor movable part of the centrifugal treating
In liquid collecting bag；
D) moving down by the indoor movable part of the centrifugal treating makes the dilution of specific quantity from dilution bag
It is input in the centrifugal treating room；
E) it is rotated by the acceleration or deceleration clockwise or counterclockwise of the centrifugal treating room, makes to be deposited on the centrifugation
Cell in processing chamber interior walls is resuspended in the dilution of input；
Cell re-suspension liquid is output to one by f) moving up by the indoor movable part of the centrifugal treating
Or in multiple collecting bags；And optionally
G) dilution of specific quantity is input in each collecting bag from dilution bag,
The amount of the cell re-suspension liquid wherein inputted into multiple collecting bags is same or different, and to multiple collections
The amount of the dilution inputted in bag is same or different.
8. the method according to the 7th, further includes, in step c) and d) between, using dilution to be deposited on from
The heart handles the step of cell in chamber interior walls carries out one or many cleanings.
9. using the density of system call interception fluid sample containing cell according to any one of 1-5 and/or
The method of volume, which comprises
A) moving down by the indoor movable part of the centrifugal treating keeps containing for the specific quantity in sample sack thin
The fluid sample of born of the same parents enters in the centrifugal treating room；
B) further moving down by the indoor movable part of the centrifugal treating makes the dilution of specific quantity from dilute
Liquid bag is released to be input in the centrifugal treating room；
C) by the rotation of the centrifugal treating room, by the indoor liquid blending of the centrifugal treating；
The liquid of mixing is output to one by d) moving up by the indoor movable part of the centrifugal treating
Or in multiple collecting bags；And optionally
E) dilution of specific quantity is input in each collecting bag from dilution bag,
The amount of the dilution wherein inputted into multiple collecting bags is same or different.
10. using the density of system call interception fluid sample containing cell according to any one of 1-5 and/or
The method of volume, which comprises
Enter the fluid sample containing cell of the specific quantity in sample sack in one or more of collecting bags；With
And optionally
B) dilution of specific quantity is input in each collecting bag from dilution bag,
The amount of the fluid sample wherein inputted into multiple collecting bags is same or different, and to multiple collecting bags
The amount of the dilution of middle input is same or different.
In one aspect, in the system of the present invention, the hollow centrifugal treating room can by with rotary drive mechanism
It engages and rotates around the axis of rotation.Centrifugal treating room, which has, to be entered for liquid to be processed and the discharge of treated liquid enters
Mouth/outlet.The centrifugal treating room has substantial cylindrical shape, and receiving is axially movable in substantial cylindrical wall
Component, such as piston.What the substantial cylindrical wall and the component that is axially movable limited centrifugal treating room can variant
Long-pending separated space, wherein the axial movement of movable member changes the volume of separated space, to keep liquid into or out
The centrifugal treating room.The example of centrifugal treating room for use in the present invention can participate in, for example, (it passes through reference to WO9715399
It is hereby incorporated by).
In one aspect, system of the invention constitutes closed gnotobasis, the various bags, centrifugal treating room and pipeline
And valve is pre-connection, or can be by sterile connector or connector or with other sterile connection sides as known in the art
Formula carries out sterile connection each other.
In one aspect, in the system of the present invention, the various bags can by it is as known in the art be usually used in it is similar
The a variety of materials of purposes are made, and form and capacity can be determined according to actual needs by those skilled in the art.
In one aspect, in the system of the present invention, as it is known to the person skilled in the art, by computer program control from
Dynamic swivel tee valve can be replaced by corresponding threeway and the combination of pipe clamp.The pipe clamp can be the pipe by manual control
Folder or the automatic pinch valve controlled by computer program.
In one aspect, in the system of the present invention, it is preferable that from the angle for improving system automation degree, In
It is respectively set between sample sack, waste liquid collecting bag and centrifugal treating room and between centrifugal treating room, dilution bag and collection device
Set the automatic swivel tee valve that can be controlled by computer program.In one aspect, in the system of the present invention, it is preferable that
From reducing cost and make the collection device of system that there is expansibility the number of collecting bag (can according to need increase or decrease)
Angle set out, being opened or closed for the pipeline for leading to each collecting bag is controlled using the combination of threeway and pipe clamp.
In one aspect, in the system of the present invention, it is determined by weighing sensor from sample sack or dilution bag
The amount of the liquid of discharge, and the amount for the liquid being input in waste fluid bag or collecting bag is determined by weighing sensor.With this
Mode can also determine the amount for entering or leaving the liquid of centrifugal treating room.
In one aspect, system of the invention can be used for handling biology and medical domain frequently involves it is various containing
The fluid sample of cell, including various biofluids, such as various blood or blood derivatives sample, and it is in culture medium or life
Manage the cell sample in the solution such as salt water.In the case where processing biofluid such as various blood or blood derivatives sample, this
The separation that cell may be implemented in the system of invention (makes cell deposition on the inner wall of centrifugal treating room, then by waste liquid after centrifugation
Discharge, the and optionally cell of deposition is resuspended and is washed) and obtain there are the more of different volumes and different densities
Cell sample after a processing.
In one aspect, the dilution in the dilution bag of system for use in the present invention can be commonly used in the art
Be suitable as the various solution of the liquid-carrier of cell, including for example suitable for the various buffers of living cells, physiological saline,
Various culture mediums etc. for maintaining cell to grow.
In one aspect, can use cell contained in the sample of system processing of the invention may, for example, be biology
The various cells frequently involved with medical domain, such as stem cell, lymphocyte etc..
Brief description
Fig. 1 shows the density and/or body for being used to adjust fluid sample containing cell of an example according to the present invention
The schematic diagram of long-pending system.
Fig. 2 shows the density and/or body for being used to adjust fluid sample containing cell of an example according to the present invention
The schematic diagram of long-pending system.
Fig. 3 show an example according to the present invention using the density of system call interception sample of the invention and the side of volume
The flow chart of method.
Fig. 4 show an example according to the present invention using the density of system call interception sample of the invention and the side of volume
The flow chart of method.
Fig. 5 show an example according to the present invention using the density of system call interception sample of the invention and the side of volume
The flow chart of method.
Fig. 6 show an example according to the present invention using the density of system call interception sample of the invention and the side of volume
The flow chart of method.
Specific embodiment
Fig. 1 shows the density and/or body for being used to adjust fluid sample containing cell of an example according to the present invention
The schematic diagram of long-pending system 100.System 100 includes: sample sack 101；Waste liquid collecting bag 102；Dilution bag 103；Collection device
104, the collection device includes multiple collecting bags 105 in parallel；The hollow centrifugal treating room that can be rotated around rotary shaft
106, accommodate in the centrifugal treating room with the cooperation of the inner wall of the centrifugal treating room be axially movable with change it is described from
The volume of the processing space of heart process chamber is (preferably living to the movable part for making liquid enter or leave the centrifugal treating room
Plug) 107, the top of the centrifugal treating room has the inlet/outlet 108 for allowing liquid inflow/outflow；For connecting
State the pipe-line system of bag and the centrifugal treating room, the pipe-line system include two triple valves (109 and 110) and threeway and
The combination of pipe clamp is wherein provided with the first triple valve 109 between sample sack 101, waste liquid collecting bag 102 and centrifugal treating room 106,
And the second triple valve 110 is provided between centrifugal treating room 106, dilution bag 103 and collection device 104；And multiple titles
It retransmits sensor (111,112 and 113), weighing sensor 111 is used to monitor the weight change of sample sack 101, weighing sensor 112
For monitoring the weight change of dilution bag 103, weighing sensor 113 is used to monitor weight change (the weighing biography of collecting bag 105
Sensor 113 can be a weighing sensor connecting simultaneously with multiple collecting bags, or it is alternatively possible to be and multiple collections
Multiple weighing sensors that bag is separately connected), and can also optionally be arranged within system 100 for monitoring waste liquid collecting bag
The weighing sensor (not shown) of 102 weight change.
System 100 constitutes closed gnotobasis.Dilution bag 103 and collection device 104 are arranged such that dilution
Dilution in bag 103 can be entered in each collecting bag 105 of collection device 104 by gravity, or be wriggled by setting
(not shown) is pumped to realize input of the dilution in dilution bag 103 into each collecting bag 105 of collection device 104.Appoint
The fluid sample that selection of land, sample sack 101 and collection device 104 are arranged such that in sample sack 101 can be entered by gravity
In each collecting bag 105 of collection device 104, or the liquid in sample sack 101 realized by setting peristaltic pump (not shown)
Input of the body sample into each collecting bag 105 of collection device 104.
In Fig. 1, controlled using multiple pipe clamps 114 each collecting bag 105 from liquid to collection device 104 inflow.Institute
The number of pipe clamp 114 can depend on the number of the collecting bag 105 in collection device 104, the input to each collecting bag
It can be controlled by using a pipe clamp 114.As understood by a person skilled in the art, in Fig. 1 to control liquid to receipts
The threeway of the inflow of each collecting bag 105 of acquisition means 104 and the combination of pipe clamp 114 can also be replaced by as shown in Figure 2
Triple valve 115, such as can be by the automatic swivel tee valve of process control.It is controlled as shown in Figure 1 using multiple pipe clamps 114
Liquid processed can increase the expansibility of the collection device 104 of system 100 to the inflow of each collecting bag 105 of collection device 104
(can according to need the number for easily increasing or decreasing collecting bag 105), and multiple triple valves are used as shown in Figure 2
115 can increase the degree of automation of whole system 100 to control liquid to the inflow of each collecting bag 105 of collection device 104.
Fig. 3 show an example according to the present invention using the density of system call interception sample of the invention and the side of volume
The flow chart of method.In the case where cell has been in target solution such as physiological saline or culture medium, in order to be had
The sample of different densities and volume, can use centrifugal treating room makes the cell deposition in sample on the inner wall of process chamber, so
Afterwards from a certain amount of clear liquid is discharged in process chamber and the cell of deposition is resuspended in remaining clear liquid to obtaining the dense of cell
Concentrate is input in each collecting bag by contracting liquid later, last as needed, and the dilution of specific quantity is input to each collecting bag
In to obtain needed for concentration and volume cell sample.As shown in figure 3, the method as example includes: to pass through centrifugal treating
Indoor piston moves down, and the fluid sample containing cell in sample sack is made to enter in centrifugal treating room (301)；Pass through centrifugation
The rotating centrifugal of process chamber makes the cell deposition in the indoor fluid sample of centrifugal treating on the inner wall of centrifugal treating room
(302)；By moving up for the indoor piston of centrifugal treating, the clear liquid after the centrifugation of specific quantity is drained into waste liquid collecting bag
(303)；By the acceleration or deceleration rotation clockwise or counterclockwise of centrifugal treating room, make to be deposited in centrifugal treating chamber interior walls
Cell be resuspended in remaining clear liquid (304)；By moving up for the indoor piston of centrifugal treating, cell re-suspension liquid is output to
In one or more collecting bags (305)；And the dilution of specific quantity is optionally input to each collecting bag from dilution bag
In (306).The amount of the cell re-suspension liquid inputted into multiple collecting bags can be same or different, and to multiple collections
The amount of the dilution inputted in bag can be same or different.
Fig. 4 show an example according to the present invention using the density of system call interception sample of the invention and the side of volume
The flow chart of method.When needing to separate cell from its background solution (for example, when cell is in various biology streams
When in body such as various blood or blood derivatives sample), there is different densities and volume in order to obtain to be in target solution
Cell sample, can use centrifugal treating room makes the cell deposition in sample on the inner wall of process chamber, then from process chamber
The cell of deposition is simultaneously resuspended in from dilution bag and is input to processing by the whole clear liquid (ingredient containing removal in need) of middle discharge
It is later that this is thin to obtain cell re-suspension liquid in the dilution (target solution) of specific quantity (depending on actual needs) in room
Born of the same parents' re-suspension liquid is input in each collecting bag, last as needed, and the dilution (target solution) of specific quantity is input to each collection
The cell sample of required concentration and volume is obtained in bag.As shown in figure 4, the method as example includes: by centrifugation
Moving down for indoor piston is managed, the fluid sample containing cell in sample sack is made to enter in centrifugal treating room (401)；By from
The rotating centrifugal of heart process chamber makes the cell deposition in the indoor fluid sample of centrifugal treating on the inner wall of centrifugal treating room
(402)；By moving up for the indoor piston of centrifugal treating, whole clear liquids after centrifugation are drained into waste liquid collecting bag (403)；
By moving down for the indoor piston of centrifugal treating, it is input to the dilution of specific quantity in centrifugal treating room from dilution bag
(404)；By the acceleration or deceleration rotation clockwise or counterclockwise of centrifugal treating room, make to be deposited in centrifugal treating chamber interior walls
Cell be resuspended in the dilution of input (405)；By moving up for the indoor piston of centrifugal treating, cell re-suspension liquid is exported
Into one or more collecting bags (406)；And the dilution of specific quantity is optionally input to each collection from dilution bag
In bag (407).The amount of the cell re-suspension liquid inputted into multiple collecting bags can be same or different, and to multiple receipts
The amount of the dilution inputted in collection bag can be same or different.It optionally, can be between step (403) and (404)
One or many cleanings are carried out to the cell being deposited in centrifugal treating chamber interior walls using dilution (target solution).
Fig. 5 show an example according to the present invention using the density of system call interception sample of the invention and the side of volume
The flow chart of method.In the case where directly dilute to cell sample, by raw sample and it can be come from by centrifugal treating room
The dilution of the specific quantity of dilution bag is mixed to realize dilution effect, is then input to the fluid sample after dilution respectively
In collecting bag, and it is last as needed, the dilution of specific quantity is input in each collecting bag further different to carry out
The dilution of degree.As shown in figure 5, the method as example includes: moving down by the indoor piston of centrifugal treating, make sample sack
In the fluid sample containing cell of specific quantity enter in centrifugal treating room (501)；Pass through the indoor piston of centrifugal treating
It further moves down, the dilution of specific quantity is made to be input in centrifugal treating room (502) from dilution bag；Pass through centrifugal treating room
Rotation, by the indoor liquid blending of centrifugal treating (503)；By moving up for the indoor piston of centrifugal treating, by the liquid of mixing
(504) are output in one or more collecting bags；And optionally the dilution of specific quantity is input to from dilution bag each
In collecting bag (505).The amount of the dilution inputted into multiple collecting bags can be same or different.
Fig. 6 show an example according to the present invention using the density of system call interception sample of the invention and the side of volume
The flow chart of method.It, can also be by the raw sample of specific quantity directly from sample in the case where directly dilute to cell sample
(by the rotation for controlling two triple valves 109 and 110 as shown in Figure 1), then as needed bag is input in collecting bag
The dilution of specific quantity is input to the diluted cell sample that required concentration and volume are obtained in each collecting bag.Such as Fig. 6
Shown, the method as example includes: that the fluid sample containing cell of the specific quantity in sample sack is made to enter one or more
In collecting bag (601)；And optionally by the dilution of specific quantity from being input in each collecting bag (602) in dilution bag.To
The amount of the fluid sample inputted in multiple collecting bags can be same or different, and input into multiple collecting bags dilute
The amount for releasing liquid can be same or different.
The method of the above specific embodiment description is only as the sample treatment that can use system implementation of the invention
The example of method, and it is not construed as the limitation to can use the sample treatment that system of the invention is implemented.According to this
Literary disclosure, it will be understood by those skilled in the art that can be implemented with approach described above not using system of the invention
The mutation of same various samples processing method or approach described above.
Claims (10)
1. for adjusting the density of fluid sample containing cell and/or the system of volume, the system comprises:
Sample sack；
Waste liquid collecting bag；
Dilution bag；
Collection device, the collection device include one or more collecting bags；
The hollow centrifugal treating room that can be rotated around rotary shaft accommodates and the centrifugal treating room in the centrifugal treating room
Inner wall cooperation be axially movable with change the volume of the processing space of the centrifugal treating room to make liquid enter or from
The movable part (preferably piston) of the centrifugal treating room is opened, the top of the centrifugal treating room has for allowing liquid flow
The inlet/outlet for entering/flowing out；
For connecting the pipe-line system of the bag and the centrifugal treating room, the pipe-line system includes multiple triple valves or corresponding
Threeway and pipe clamp combination, wherein being provided between the sample sack, the waste liquid collecting bag and the centrifugal treating room
The combination of one triple valve or corresponding threeway and pipe clamp, and centrifugal treating room, the dilution bag and collection dress
The combination of the second triple valve or corresponding threeway and pipe clamp is provided between setting；And
Multiple weighing sensors, the weighing sensor is for monitoring the sample sack, dilution bag, collecting bag, and/or waste liquid
Collecting bag is because of the weight change caused by being discharged or receiving liquid；
Wherein the system of the density and/or volume for adjusting fluid sample containing cell constitutes closed asepsis ring
Border.
2. the system of claim 1, wherein the dilution bag and the collection device are arranged such that the dilution bag
In dilution can be entered in the collection device by gravity, or realized in the dilution bag by peristaltic pump
Input of the dilution into the collection device, and optionally, the sample sack and the collection device are arranged such that
Fluid sample in the sample sack can be entered in the collection device by gravity, or be realized by peristaltic pump described
Input of the fluid sample into the collection device in sample sack.
3. the system of claim 1, wherein the collection device includes multiple collecting bags in parallel, and by with collecting bag number
Inflow of the combination control liquid of the triple valve of mesh corresponding number or corresponding threeway and pipe clamp to each collecting bag.
4. the system of claim 3, wherein the multiple collecting bag is connect with one or more weighing sensors.
5. for adjusting the density of fluid sample containing cell and/or the system of volume, the system comprises:
Sample sack；
Waste liquid collecting bag；
Dilution bag；
Collection device, the collection device include multiple collecting bags in parallel；
The hollow centrifugal treating room that can be rotated around rotary shaft accommodates and the centrifugal treating room in the centrifugal treating room
Inner wall cooperation be axially movable with change the volume of the processing space of the centrifugal treating room to make liquid enter or from
The movable part (preferably piston) of the centrifugal treating room is opened, the top of the centrifugal treating room has for allowing liquid flow
The inlet/outlet for entering/flowing out；
For connecting the pipe-line system of the bag and the centrifugal treating room, the pipe-line system includes multiple triple valves or corresponding
Threeway and pipe clamp combination, wherein being provided between the sample sack, the waste liquid collecting bag and the centrifugal treating room
One triple valve, and the second triple valve is provided between the centrifugal treating room, the dilution bag and the collection device；With
And
Multiple weighing sensors, the weighing sensor is for monitoring the sample sack, dilution bag, collecting bag, and/or waste liquid
Collecting bag is because of the weight change caused by being discharged or receiving liquid；
Wherein the system of the density and/or volume for adjusting fluid sample containing cell constitutes closed asepsis ring
Border, the dilution bag and the collection device be arranged such that the dilution in the dilution bag can by gravity into
Enter in the collection device, or realizes by peristaltic pump dilution in the dilution bag into the collection device
Input, and optionally, the sample sack and the collection device are arranged such that the fluid sample energy in the sample sack
Enough entered in the collection device by gravity, or realizes by peristaltic pump fluid sample in the sample sack to described
Input in collection device controls inflow of the liquid to each collecting bag, and the multiple collecting bag and a title by pipe clamp
Retransmit sensor connection.
6. utilizing the density of the system call interception fluid sample containing cell of any one of claim 1-5 and/or the side of volume
Method, which comprises
A) moving down by the indoor movable part of the centrifugal treating makes the fluid sample containing cell in sample sack
Into in the centrifugal treating room；
B) by the rotating centrifugal of the centrifugal treating room, the cell deposition in the indoor fluid sample of the centrifugal treating is made to exist
On the inner wall of the centrifugal treating room；
Clear liquid after the centrifugation of specific quantity is drained into useless by c) moving up by the indoor movable part of the centrifugal treating
In liquid collecting bag；
D) it is rotated by the acceleration or deceleration clockwise or counterclockwise of the centrifugal treating room, makes to be deposited on the centrifugal treating
Cell in chamber interior walls is resuspended in remaining clear liquid；
Cell re-suspension liquid is output to one or more by e) moving up by the indoor movable part of the centrifugal treating
In a collecting bag；And optionally
F) dilution of specific quantity is input in each collecting bag from dilution bag,
The amount of the cell re-suspension liquid wherein inputted into multiple collecting bags is same or different, and into multiple collecting bags
The amount of the dilution of input is same or different.
7. utilizing the density of the system call interception fluid sample containing cell of any one of claim 1-5 and/or the side of volume
Method, which comprises
A) moving down by the indoor movable part of the centrifugal treating makes the fluid sample containing cell in sample sack
Into in the centrifugal treating room；
B) by the rotating centrifugal of the centrifugal treating room, the cell deposition in the indoor fluid sample of the centrifugal treating is made to exist
On the inner wall of the centrifugal treating room；
Whole clear liquids after centrifugation are drained into waste liquid and received by c) moving up by the indoor movable part of the centrifugal treating
Collect in bag；
D) moving down by the indoor movable part of the centrifugal treating inputs the dilution of specific quantity from dilution bag
Into the centrifugal treating room；
E) it is rotated by the acceleration or deceleration clockwise or counterclockwise of the centrifugal treating room, makes to be deposited on the centrifugal treating
Cell in chamber interior walls is resuspended in the dilution of input；
Cell re-suspension liquid is output to one or more by f) moving up by the indoor movable part of the centrifugal treating
In a collecting bag；And optionally
G) dilution of specific quantity is input in each collecting bag from dilution bag,
The amount of the cell re-suspension liquid wherein inputted into multiple collecting bags is same or different, and into multiple collecting bags
The amount of the dilution of input is same or different.
8. method for claim 7 further includes, in step c) and d) between, using dilution to being deposited on centrifugal treating room
The step of cell on inner wall carries out one or many cleanings.
9. utilizing the density of the system call interception fluid sample containing cell of any one of claim 1-5 and/or the side of volume
Method, which comprises
A) moving down by the indoor movable part of the centrifugal treating makes the containing cell of the specific quantity in sample sack
Fluid sample enters in the centrifugal treating room；
B) further moving down by the indoor movable part of the centrifugal treating, makes the dilution of specific quantity from dilution
Bag is input in the centrifugal treating room；
C) by the rotation of the centrifugal treating room, by the indoor liquid blending of the centrifugal treating；
The liquid of mixing is output to one or more by d) moving up by the indoor movable part of the centrifugal treating
In a collecting bag；And optionally
E) dilution of specific quantity is input in each collecting bag from dilution bag,
The amount of the dilution wherein inputted into multiple collecting bags is same or different.
10. utilizing the density of the system call interception fluid sample containing cell of any one of claim 1-5 and/or the side of volume
Method, which comprises
Enter the fluid sample containing cell of the specific quantity in sample sack in one or more of collecting bags；And
Optionally
B) dilution of specific quantity is input in each collecting bag from dilution bag
The amount of the fluid sample wherein inputted into multiple collecting bags is same or different, and defeated into multiple collecting bags
The amount of the dilution entered is same or different.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910594172.9A CN110456033A (en) | 2019-07-03 | 2019-07-03 | For adjusting the density of fluid sample containing cell and/or the system of volume |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910594172.9A CN110456033A (en) | 2019-07-03 | 2019-07-03 | For adjusting the density of fluid sample containing cell and/or the system of volume |
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| Publication Number | Publication Date |
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| CN110456033A true CN110456033A (en) | 2019-11-15 |
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ID=68481940
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| Application Number | Title | Priority Date | Filing Date |
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|---|---|---|---|---|
| CN1331610A (en) * | 1998-12-24 | 2002-01-16 | 生物安全股份有限公司 | Blood separation system, in particular for concentrating hematopoietic stem cells |
| CN101620218A (en) * | 2008-07-02 | 2010-01-06 | 上海奇康生物科技有限公司 | Method for treating ingredients of biological fluid |
| CN201510941U (en) * | 2009-09-22 | 2010-06-23 | 上海交通大学医学院附属第九人民医院 | Marrow mesenchymal stem cell centrifugation and enrichment sorter |
| WO2015160320A1 (en) * | 2014-04-15 | 2015-10-22 | Promedart Biyoteknoloji Ve Ozel Saglik Hizmetleri Sanayi Ticaret Limited Sirketi | A separation system |
| CN109321457A (en) * | 2018-10-17 | 2019-02-12 | 深圳市深研生物科技有限公司 | A kind of equipment and its application preparing cell |
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2019
- 2019-07-03 CN CN201910594172.9A patent/CN110456033A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1331610A (en) * | 1998-12-24 | 2002-01-16 | 生物安全股份有限公司 | Blood separation system, in particular for concentrating hematopoietic stem cells |
| CN101620218A (en) * | 2008-07-02 | 2010-01-06 | 上海奇康生物科技有限公司 | Method for treating ingredients of biological fluid |
| CN201510941U (en) * | 2009-09-22 | 2010-06-23 | 上海交通大学医学院附属第九人民医院 | Marrow mesenchymal stem cell centrifugation and enrichment sorter |
| WO2015160320A1 (en) * | 2014-04-15 | 2015-10-22 | Promedart Biyoteknoloji Ve Ozel Saglik Hizmetleri Sanayi Ticaret Limited Sirketi | A separation system |
| CN109321457A (en) * | 2018-10-17 | 2019-02-12 | 深圳市深研生物科技有限公司 | A kind of equipment and its application preparing cell |
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Application publication date: 20191115 |