CN110447576A - A kind of Fugu rubripes triploid induction method based on hydrostatic platen press - Google Patents
A kind of Fugu rubripes triploid induction method based on hydrostatic platen press Download PDFInfo
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- CN110447576A CN110447576A CN201910861000.3A CN201910861000A CN110447576A CN 110447576 A CN110447576 A CN 110447576A CN 201910861000 A CN201910861000 A CN 201910861000A CN 110447576 A CN110447576 A CN 110447576A
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- China
- Prior art keywords
- fugu rubripes
- hydrostatic
- triploid
- fertilized eggs
- ovum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The Fugu rubripes triploid induction method based on hydrostatic platen press that the present invention relates to a kind of, belongs to Fugu rubripes genetic breeding field.Fugu rubripes triploid induction method of the present invention includes the following steps: the ovum of the sperm for taking Fugu rubripes maturation milter and Fugu rubripes raun maturation；Sperm is added in mature ovum, adds seawater, obtains fertilized eggs；Fertilized eggs are transferred in the pressurizing chamber of hydrostatic press, press 30-80MPa, continues 2-10min；By hydrostatic pressing, treated that fertilized eggs are transferred in incubator hatches.The method of the invention induced triploid success rate is high, and hatching rate is high, easy to operate, saves the time, has good practicability and operability, can be used as industrial application measure.
Description
Technical field
The Fugu rubripes triploid induction method based on hydrostatic platen press that the present invention relates to a kind of belongs to Fugu rubripes something lost
Pass breeding field.
Background technique
Fugu rubripes (Takifugu rubripes) belongs to Tetraodontiformes, Molidae, and red fin Puffer category is distributed in North Pacific
Western Japan, the Korea peninsula and coastal area of china, sees the yellow Bohai Sea and the East Sea in China.Currently, China coast has had found 17
Kind Fugu, Fugu rubripes are individual maximum, the higher one kind of economic value in the category.Because its fine and tender taste, delicious flavour,
Protein is high, full of nutrition, deep to be liked by numerous gourmets, especially famous with delicious raw fish.Fugu rubripes muscle can be with
Edible, still, gonad, internal organ, skin etc. are toxic.The ovary percentage of liveweight ratio of adult female is up to 20% or so, ovum
Nest is toxic, cannot eat, it is therefore necessary to all throw away, lead to the ratio sharp fall of edible part, be from operation
Huge loss.However the spermary of spermary milter is not only nontoxic or first-class delicacies, is the most valuable part of river Puffer fish.
It is very difficult that Fugu rubripes distinguishes male and female from the appearance, is regardless of male and female, with identical price now therefore
Sale.In the mature period, veteran, which touches fish belly portion, can distinguish male and female, but also not be 100% accurate.It is selling
When ten hundreds of quantity, male and female are distinguished in such a way that every tail touches belly and need the huge artificial and time, are uneconomic.
In addition, the method by molecular labeling can be with precise Identification male and female, but ten hundreds of quantity is identified by DNA method, from
Consider on time and in cost and unpractical.
It is improvement one is milter by its all-male, is all become without raun as the method for improving culture efficiency
The very wise move of kind；Another method is to be induced into triploid, it is made not have sexual gland, both without spermary or without ovary, is subtracted
The loss of ovary is lacked, a kind of this valuable modification method of also can yet be regarded as.
The sexual gland of triploid fish is usually agensis, even if the development of some types is also irreproducible offspring's.Cause
This, triploid fish have characteristics that based on gonad agensis, needed for energy be diverted to grow, therefore triploid
It is generally faster than diploid growth, there is very big Exploitative potential in aquaculture；Based on sterility, three times of artificial induction fish
Body is individually cultivated or is discharged in natural water area, has the effect of preventing population excessive multiplication；Fish muscle quality is general
It is mature with sexual gland, the phenomenon that quality decline can be generated, in the case where infertility, can with the adverse effect of removing property gland maturity,
Form the effect for guaranteeing meat.For toxic river Puffer class, ovary is completely inedible, after induced triploid, ovarian dysgenesis,
Energy loss is reduced, and this portion of energy is converted into edible part, actually one of reasonable measure.
Summary of the invention
The present invention uses hydrostatic platen press induced triploid, the spy high with inductivity, hatching rate is high and weed survival rate is high
Point.
The present invention provides a kind of Fugu rubripes triploid induction method based on hydrostatic platen press, the Fugu rubripes
Triploid induction method includes the following steps: the ovum of the sperm for taking Fugu rubripes maturation milter and Fugu rubripes raun maturation
Son；Sperm is added in mature ovum, adds seawater, obtains fertilized eggs；Fertilized eggs are transferred to the pressurization of hydrostatic press
In chamber, press 30-80MPa, continues 2-10min；By hydrostatic pressing, treated that fertilized eggs are transferred in incubator hatches.
The present invention is preferably sperm and the Fugu rubripes for utilizing induced spawning method to obtain Fugu rubripes maturation milter
The ovum of raun maturation.
The present invention preferably fertilization water temperature is 15-20 DEG C, pH 7.8-8.5, and salinity is 25-35 ‰.
2-12min after seawater is preferably added in the present invention, then fertilized eggs are transferred in the pressurizing chamber of hydrostatic press.
It is 15-20 DEG C, pH 7.8-8.5 that the present invention, which preferably hatches water temperature, and salinity is 25-35 ‰.
The triploid fish shoot survival percent that the present invention induces is 80% or more.
The invention has the following beneficial effects:
The method of the invention induced triploid success rate is high, and hatching rate is high, easy to operate, saves the time, has fine
Practicability and operability, can be used as industrial application measure.
Detailed description of the invention
2 width of attached drawing of the present invention,
Fig. 1 is the diploid peak figure of embodiment 1；
Fig. 2 is the triploid peak figure of embodiment 1.
Specific embodiment
Following non-limiting embodiments can with a person of ordinary skill in the art will more fully understand the present invention, but not with
Any mode limits the present invention.
Embodiment 1
A kind of Fugu rubripes triploid induction method based on hydrostatic platen press, the Fugu rubripes triploid induction side
Method includes the following steps:
The sperm of Fugu rubripes maturation milter and the ovum of Fugu rubripes raun maturation are obtained using induced spawning method
Son；
Sperm is added in mature ovum, stirs, contacts sperm sufficiently with ovum, adds seawater, activates ovum,
Fertilized eggs are obtained, fertilization water temperature is 17 DEG C, pH 8.0, and salinity is 30 ‰；
4min after addition seawater, then fertilized eggs are transferred in the pressurizing chamber of hydrostatic press, press 40MPa, continues 2min；
By hydrostatic pressing, treated that fertilized eggs are transferred in incubator hatches, and hatching water temperature is 17 DEG C, pH 8.0, salinity
It is 30 ‰.
Triploid rate is 80% or more.
Embodiment 2
A kind of Fugu rubripes triploid induction method based on hydrostatic platen press, the Fugu rubripes triploid induction side
Method includes the following steps:
The sperm of Fugu rubripes maturation milter and the ovum of Fugu rubripes raun maturation are obtained using induced spawning method
Son；
Sperm is added in mature ovum, stirs, contacts sperm sufficiently with ovum, adds seawater, activates ovum,
Fertilized eggs are obtained, fertilization water temperature is 18 DEG C, pH 7.8, and salinity is 28 ‰；
4min after addition seawater, then fertilized eggs are transferred in the pressurizing chamber of hydrostatic press, press 50MPa, continues 5min；
By hydrostatic pressing, treated that fertilized eggs are transferred in incubator hatches, and hatching water temperature is 18 DEG C, pH 7.8, salinity
It is 28 ‰.
Triploid rate is 90% or more.
Embodiment 3
A kind of Fugu rubripes triploid induction method based on hydrostatic platen press, the Fugu rubripes triploid induction side
Method includes the following steps:
The sperm of Fugu rubripes maturation milter and the ovum of Fugu rubripes raun maturation are obtained using induced spawning method
Son；
Sperm is added in mature ovum, stirs, contacts sperm sufficiently with ovum, adds seawater, activates ovum,
Fertilized eggs are obtained, fertilization water temperature is 20 DEG C, pH 8.1, and salinity is 31 ‰；
6min after addition seawater, then fertilized eggs are transferred in the pressurizing chamber of hydrostatic press, press 40MPa, continues 2min；
By hydrostatic pressing, treated that fertilized eggs are transferred in incubator hatches, and hatching water temperature is 20 DEG C, pH 8.1, salinity
It is 31 ‰.
Triploid rate is 90% or more.
Embodiment 4
A kind of Fugu rubripes triploid induction method based on hydrostatic platen press, the Fugu rubripes triploid induction side
Method includes the following steps:
The sperm of Fugu rubripes maturation milter and the ovum of Fugu rubripes raun maturation are obtained using induced spawning method
Son；
Sperm is added in mature ovum, stirs, contacts sperm sufficiently with ovum, adds seawater, activates ovum,
Fertilized eggs are obtained, fertilization water temperature is 18 DEG C, pH 8.0, and salinity is 31 ‰；
6min after addition seawater, then fertilized eggs are transferred in the pressurizing chamber of hydrostatic press, press 70MPa, continues 8min；
By hydrostatic pressing, treated that fertilized eggs are transferred in incubator hatches, and hatching water temperature is 18 DEG C, pH 8.0, salinity
It is 31 ‰.
Triploid rate is 90% or more.
Embodiment 5
A kind of Fugu rubripes triploid induction method based on hydrostatic platen press, the Fugu rubripes triploid induction side
Method includes the following steps:
The sperm of Fugu rubripes maturation milter and the ovum of Fugu rubripes raun maturation are obtained using induced spawning method
Son；
Sperm is added in mature ovum, stirs, contacts sperm sufficiently with ovum, adds seawater, activates ovum,
Fertilized eggs are obtained, fertilization water temperature is 19 DEG C, pH 8.0, and salinity is 28 ‰；
8min after addition seawater, then fertilized eggs are transferred in the pressurizing chamber of hydrostatic press, press 50MPa, continues 2min；
By hydrostatic pressing, treated that fertilized eggs are transferred in incubator hatches, and hatching water temperature is 19 DEG C, pH 8.0, salinity
It is 28 ‰.
Triploid rate about 90%.
Embodiment 6
A kind of Fugu rubripes triploid induction method based on hydrostatic platen press, the Fugu rubripes triploid induction side
Method includes the following steps:
The sperm of Fugu rubripes maturation milter and the ovum of Fugu rubripes raun maturation are obtained using induced spawning method
Son；
Sperm is added in mature ovum, stirs, contacts sperm sufficiently with ovum, adds seawater, activates ovum,
Fertilized eggs are obtained, fertilization water temperature is 21 DEG C, pH 8.0, and salinity is 30 ‰；
8min after addition seawater, then fertilized eggs are transferred in the pressurizing chamber of hydrostatic press, press 70MPa, continues 8min；
By hydrostatic pressing, treated that fertilized eggs are transferred in incubator hatches, and hatching water temperature is 21 DEG C, pH 8.0, salinity
It is 30 ‰.
Triploid rate about 80%.
Claims (5)
1. a kind of Fugu rubripes triploid induction method based on hydrostatic platen press, it is characterised in that: the Fugu rubripes three
Times body abductive approach includes the following steps:
Take the sperm of Fugu rubripes maturation milter and the ovum of Fugu rubripes raun maturation；
Sperm is added in mature ovum, adds seawater, obtains fertilized eggs；
Fertilized eggs are transferred in the pressurizing chamber of hydrostatic press, press 30-80MPa, continues 2-10min；
By hydrostatic pressing, treated that fertilized eggs are transferred in incubator hatches.
2. the Fugu rubripes triploid induction method based on hydrostatic platen press according to claim 1, it is characterised in that: utilize
Induced spawning method obtains the sperm of Fugu rubripes maturation milter and the ovum of Fugu rubripes raun maturation.
3. the Fugu rubripes triploid induction method based on hydrostatic platen press according to claim 2, it is characterised in that: fertilization
Water temperature is 15-20 DEG C, pH 7.8-8.5, and salinity is 25-35 ‰.
4. the Fugu rubripes triploid induction method based on hydrostatic platen press according to claim 3, it is characterised in that: be added
2-12min after seawater, then fertilized eggs are transferred in the pressurizing chamber of hydrostatic press.
5. the Fugu rubripes triploid induction method based on hydrostatic platen press according to claim 4, it is characterised in that: hatching
Water temperature is 15-20 DEG C, pH 7.8-8.5, and salinity is 25-35 ‰.
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| CN201910861000.3A CN110447576A (en) | 2019-09-11 | 2019-09-11 | A kind of Fugu rubripes triploid induction method based on hydrostatic platen press |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114557296A (en) * | 2022-02-28 | 2022-05-31 | 中国水产科学研究院黄海水产研究所 | Method for inducing scophthalmus maximus triploid in batches by hydrostatic pressure method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1836504A (en) * | 2006-04-13 | 2006-09-27 | 厦门大学 | Takifugu bimaculatus triploid induction method |
| KR20110017823A (en) * | 2010-07-27 | 2011-02-22 | 주식회사 씨에버 | Method of producing a viable tetraploid oyster |
| CN102246708A (en) * | 2010-05-18 | 2011-11-23 | 中国水产科学院研究院 | Triploid induction method of cynoglossus semilaevis |
| CN102823522A (en) * | 2011-06-17 | 2012-12-19 | 河北省水产研究所 | Triploid induction of Takifugu flavidus and Takifugu rubripes |
| CN109220902A (en) * | 2018-09-26 | 2019-01-18 | 中国海洋大学 | A kind of producing method for seed of hydrostatic pressing induction rainbow trout triploid |
-
2019
- 2019-09-11 CN CN201910861000.3A patent/CN110447576A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1836504A (en) * | 2006-04-13 | 2006-09-27 | 厦门大学 | Takifugu bimaculatus triploid induction method |
| CN102246708A (en) * | 2010-05-18 | 2011-11-23 | 中国水产科学院研究院 | Triploid induction method of cynoglossus semilaevis |
| KR20110017823A (en) * | 2010-07-27 | 2011-02-22 | 주식회사 씨에버 | Method of producing a viable tetraploid oyster |
| CN102823522A (en) * | 2011-06-17 | 2012-12-19 | 河北省水产研究所 | Triploid induction of Takifugu flavidus and Takifugu rubripes |
| CN109220902A (en) * | 2018-09-26 | 2019-01-18 | 中国海洋大学 | A kind of producing method for seed of hydrostatic pressing induction rainbow trout triploid |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114557296A (en) * | 2022-02-28 | 2022-05-31 | 中国水产科学研究院黄海水产研究所 | Method for inducing scophthalmus maximus triploid in batches by hydrostatic pressure method |
| CN114557296B (en) * | 2022-02-28 | 2022-12-02 | 中国水产科学研究院黄海水产研究所 | Method for inducing scophthalmus maximus triploid in batches by hydrostatic pressure method |
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