CN110438089A - A kind of virus preservation liquid that can effectively save viral equal samples for a long time - Google Patents

A kind of virus preservation liquid that can effectively save viral equal samples for a long time Download PDF

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Publication number
CN110438089A
CN110438089A CN201910611068.6A CN201910611068A CN110438089A CN 110438089 A CN110438089 A CN 110438089A CN 201910611068 A CN201910611068 A CN 201910611068A CN 110438089 A CN110438089 A CN 110438089A
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virus
long time
equal samples
effectively save
liquid
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Inventor
巩赞华
巩赞博
巩赞斌
张金银
黄咏华
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HUACHENYANG (SHENZHEN) TECHNOLOGY Co Ltd
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HUACHENYANG (SHENZHEN) TECHNOLOGY Co Ltd
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Priority to CN201910611068.6A priority Critical patent/CN110438089A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/18011—Paramyxoviridae
    • C12N2760/18411—Morbillivirus, e.g. Measles virus, canine distemper
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32311—Enterovirus

Abstract

The invention discloses a kind of viruses that can effectively save viral equal samples for a long time to save liquid, it is consisted of the following compositions: calcium chloride 0.1-3.5g, potassium chloride 0.07-1.49g, sodium chloride 0.76-2.76g, magnesium chloride 0.01-0.91g, magnesium sulfate 0.01-1.93g, sugared 0.1-0.361g, potassium dihydrogen phosphate 0.01-0.72g, disodium hydrogen phosphate 0.05-0.12g, sodium bicarbonate 0.007-0.67g, bovine serum albumin(BSA) 0.1-1g, antibiotic 0.6-5.3g, 4- hydroxyethyl piperazineethanesulfonic acid 0.236-0.96g, phenol red 0.01-1g, L-cysteine hydrochloride 0.01-3.52g, Pidolidone 0.28-2.9 5g, water are settled to 100mL, and pH value is adjusted to 7.2-7.8.The invention has the beneficial effects that: it can not only be used to save clinical influenza virus, avian influenza virus (such as H7N9), Sa Qi viral (A group), measles virus equal samples, it can also be used to save mycoplasma, urea substance, Chlamydia equal samples, the scope of application is than wide, and the holding time is long, preservation effect is good, has good application value.

Description

A kind of virus preservation liquid that can effectively save viral equal samples for a long time
Technical field
The present invention relates to a kind of viruses to save liquid, and in particular to a kind of disease that can effectively save viral equal samples for a long time Poison saves liquid, belongs to medical biotechnology field.
Background technique
According to statistics, the annual flu casess in the whole world are 600,000,000-12 hundred million, wherein severe influenza is 3,000,000-500 ten thousand Example, dead -50 ten thousand people of 250,000 people, the case fatality rate of severe influenza is up to 8%.
Bird flu is the acute respiratory communicable disease as caused by some Strain of the certain hypotypes of avian influenza viruses, Wherein, people's highly pathogenic avian influenza virus (H7N9) is easy to cause severe pneumonia, and case fatality rate is very high, endangers to the physical and mental health of people Evil is very big.
Hank's liquid be influenza virus transport save common balanced salt solution (Balanced Salt Solution, BSS).It is influenced by various factors, Hank's liquid, which saves influenza virus, can only save several hours, the restrovirus shape of several hours The integrality of state will be affected, and then influence whether Viral extraction rate, if the time extends again, also easily Bacterium, fungi are bred, Hank's liquid pH value is caused to decline, when Hank's liquid pH value is less than 7.4, virus stability decline, disease The decomposition rate of poison is accelerated, so that virus is difficult to save for a long time.
As it can be seen that Hank's liquid does not ensure that the long-time of influenza virus effectively saves.
Summary of the invention
To solve the deficiencies in the prior art, the purpose of the present invention is to provide one kind can effectively save virus etc. for a long time The virus of sample saves liquid.
In order to achieve the above objectives, the present invention adopts the following technical scheme that:
A kind of virus preservation liquid that can effectively save viral equal samples for a long time, which is characterized in that aforementioned viral saves Liquid consists of the following compositions:
Calcium chloride 0.1-3.5g, potassium chloride 0.07-1.49g, sodium chloride 0.76-2.76g, magnesium chloride 0.01-0.91g, sulphur Sour magnesium 0.01-1.93g, sugar 0.1-0.361g, potassium dihydrogen phosphate 0.01-0.72g, disodium hydrogen phosphate 0.05-0.12g, bicarbonate Sodium 0.007-0.67g, bovine serum albumin(BSA) 0.1-1g, antibiotic 0.6-5.3g, 4- hydroxyethyl piperazineethanesulfonic acid 0.236- 0.96g, phenol red 0.01-1g, L-cysteine hydrochloride 0.01-3.52g, Pidolidone 0.28-2.95g, water are settled to 100mL, pH value are adjusted to 7.2-7.8.
The virus above-mentioned that viral equal samples can effectively be saved for a long time saves liquid, which is characterized in that aforementioned sugar is double The mixing of any one or two kinds in sugar and polysaccharide, wherein disaccharide preferably sucrose, the preferred glycogen of polysaccharide.
The virus above-mentioned that viral equal samples can effectively be saved for a long time saves liquid, which is characterized in that aforementioned antibiotics Including fungal antibiotic and bacteria antibiotic, wherein fungal antibiotic be in amphotericin B and nystatin any one or Two kinds of mixing, bacteria antibiotic are any one or any several mixing in vancomycin, colistin and gentamicin.
The virus above-mentioned that viral equal samples can effectively be saved for a long time saves liquid, which is characterized in that aforementioned sample packet Include virus, mycoplasma, gland substance and Chlamydia, wherein virus includes: influenza virus, Sa Qi virus A group and measles virus.
The invention has the beneficial effects that:
(1) we are added to bovine serum albumin(BSA), antibiotic and 4- hydroxyethyl piperazineethanesulfonic acid in formula, these are all The ingredient of morphology of virus can be stablized, so virus provided by the invention saves liquid can keep morphology of virus to a greater extent Integrality, so as to improve Viral extraction rate；
(2) antibiotic in formula can inhibit the growth of various bacteria and fungi, so virus provided by the invention is protected Liquid storage can be avoided destruction of the microorganisms such as bacterium, fungi to morphology of virus, so as to extend the holding time of virus；
(3) we also added L-cysteine hydrochloride and Pidolidone in formula, wherein L-cysteine hydrochloric acid Salt can accelerate the formation of glutelin, prevent viral aging, and Pidolidone is nutritional supplement, be also possible to prevent viral aging；
(4) can not only be used to save clinical influenza virus, avian influenza virus (such as H7N9), Sa Qi viral (A group), morbilli Viral equal samples can also be used to save mycoplasma, urea substance, Chlamydia equal samples, and the scope of application is than wide.
Specific embodiment
Below in conjunction with detailed description of the invention by specific embodiments.
One, it is formulated
Calcium chloride 0.1-3.5g, potassium chloride 0.07-1.49g, sodium chloride 0.76-2.76g, magnesium chloride 0.01-0.91g, sulphur Sour magnesium 0.01-1.93g, sugar 0.1-0.361g, potassium dihydrogen phosphate 0.01-0.72g, disodium hydrogen phosphate 0.05-0.12g, bicarbonate Sodium 0.007-0.67g, bovine serum albumin(BSA) 0.1-1g, antibiotic 0.6-5.3g, 4- hydroxyethyl piperazineethanesulfonic acid 0.236- 0.96g, phenol red 0.01-1g, L-cysteine hydrochloride 0.01-3.52g, Pidolidone 0.28-2.95g, water are settled to 100mL, pH value are adjusted to 7.2-7.8.
Sugar: either disaccharide (such as: sucrose), it is also possible to polysaccharide (such as: glycogen), can also be disaccharide and polysaccharide It uses simultaneously.
Antibiotic: including fungal antibiotic (such as: amphotericin B, nystatin) and bacteria antibiotic (such as: through the ages Mycin, colistin, gentamicin), the two uses simultaneously.
Water: distilled water or deionized water.
Phenol red: indicator is used to refer to the variation of the preservation effect of virus.
With Hank's liquid phase ratio, we are added to bovine serum albumin(BSA), antibiotic, 4- hydroxyethyl piperazine second again in formula Five kinds of ingredients such as sulfonic acid, L-cysteine hydrochloride and Pidolidone, this five kinds of ingredients be all can stablize morphology of virus at Point, in which:
(1) bovine serum albumin(BSA): maintaining osmotic pressure, is conducive to the stability and activity of virus；
(2) antibiotic: killing bacterium and fungi, is conducive to the stabilization of virus, prevents viral aging；
(3) 4- hydroxyethyl piperazineethanesulfonic acid: buffer maintains the stabilization of pH value, provides for virus preservation liquid stable Environment；
(4) L-cysteine hydrochloride: accelerating the formation of glutelin, prevents viral aging；
(5) Pidolidone: nutritional supplement prevents viral aging.
Since we are added to a variety of ingredients that can stablize morphology of virus in formula, so virus provided by the invention The integrality of morphology of virus can be kept to a greater extent by saving liquid, so as to improve Viral extraction rate.
In addition, antibiotic can also inhibit the growth of various bacteria and fungi, avoid because of bacterium and fungi release CO2And Caused preservation liquid pH value decline, and then the speed of virus decomposition is slowed down, so as to extend the holding time of virus.
Two, preparation method
According to formula each reagent of precise, then each reagent is dissolved with a small amount of distilled water (or deionized water), later It is settled to 1000mL with a large amount of distilled water (or deionized water) again, is shaken up, solution ph is finally adjusted to 7.2-7.8, that is, prepares It obtains virus and saves liquid.
In this embodiment, specific as follows we illustrate 4 group of formula:
Three, preservation effect
1, PCR is detected
Detection method: H7N9 virus, Sa Qi virus A group, measles virus are acquired respectively with sampling swab, each sample point It is not added to formula 1, formula 2, formula 3 and the corresponding virus of formula 4 to save in liquid, Hank's liquid does control group, then with life Salt water is managed by sample by 10 times, 100 times, 1000 times, 10000 times of dilutions, upper machine does the experiment of PCR nucleic acid susceptibility, measurement later CT value.
Testing result is as follows:
(1) H7N9 virus
10 times of dilutions 100 times of dilutions 1000 times of dilutions 10000 times of dilutions
Formula 1 20.07 24.15 28.21 30.79
Formula 2 21.08 25.20 29.13 31.20
Formula 3 20.11 24.11 29.32 30.75
Formula 4 22.16 26.02 29.63 30.69
Hank's liquid 24.47 26.86 34.51 No CT
(2) Sa Qi virus A group
(3) measles virus
10 times of dilutions 100 times of dilutions 1000 times of dilutions 10000 times of dilutions
Formula 1 21.07 25.14 28.91 30.11
Formula 2 21.08 25.30 29.17 30.87
Formula 3 20.11 24.11 28.32 29.97
Formula 4 20.16 24.30 28.63 30.01
Hank's liquid 25.55 28.54 34.52 No CT
It can be seen that virus provided by the invention, which saves liquid CT numerical value, is less than traditional Hank's liquid CT numerical value.
This explanation: virus preservation liquid Positive rate provided by the invention is higher than traditional Hank's liquid, possesses ratio Hank's liquid preferably maintains the ability of viral integrality.
2, Virus culture
Test method: H7N9 virus, Sa Qi virus A group, measles virus are acquired respectively with sampling swab, each sample point Be not added to formula 1, formula 2, formula 3 and formula 4 it is corresponding virus save liquid in saved (storage temperature is respectively: often Temperature, 4 DEG C, -20 DEG C, -70 DEG C), and 4h after being saved, 12h, 48h, 72h, 30d, 90d, the respectively 180d, 240d, 360d are inoculated into the chick embryo allantoic cavity of 10 ages in days (note: the sample under freezing needs first to thaw), so After observe 4d, record observation result.
It is following (positive+, negative -) to record result:
(1) H7N9 virus
A, the corresponding virus of formula 1 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
B, the corresponding virus of formula 2 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
C, the corresponding virus of formula 3 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
D, the corresponding virus of formula 4 saves liquid
(2) Sa Qi virus A group
A, the corresponding virus of formula 1 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
B, the corresponding virus of formula 2 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
C, the corresponding virus of formula 3 saves liquid
D, the corresponding virus of formula 4 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
(3) measles virus
A, the corresponding virus of formula 1 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
B, the corresponding virus of formula 2 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
C, the corresponding virus of formula 3 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
D, the corresponding virus of formula 4 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
It can be seen that virus provided by the invention saves liquid can effectively save virus for a long time.
3, other samples
1, PCR is detected
Detection method: mycoplasma, gland substance, Chlamydia are acquired respectively with sampling swab, each sample is added separately to Formula 1, formula 2, formula 3 and the corresponding virus of formula 4 save in liquid, and Hank's liquid does control group, then will with physiological saline For sample by 10 times, 100 times, 1000 times, 10000 times of dilutions, upper machine does the experiment of PCR nucleic acid susceptibility later, measures CT value.
Testing result is as follows:
(1) mycoplasma
(2) gland substance
10 times of dilutions 100 times of dilutions 1000 times of dilutions 10000 times of dilutions
Formula 1 20.26 24.63 28.41 30.78
Formula 2 21.12 25.13 29.11 31.07
Formula 3 20.11 24.32 28.42 30.44
Formula 4 21.09 25.21 29.13 31.49
Hank's liquid 26.50 29.52 35.93 No CT
(3) Chlamydia
10 times of dilutions 100 times of dilutions 1000 times of dilutions 10000 times of dilutions
Formula 1 20.07 24.15 28.21 29.79
Formula 2 20.08 24.20 28.13 29.95
Formula 3 21.11 25.11 28.32 30.76
Formula 4 21.16 26.02 28.63 30.69
Hank's liquid 26.55 27.54 35.52 No CT
It can be seen that it is provided by the invention virus save liquid can be very good maintain mycoplasma, gland substance, Chlamydia it is complete Whole property, therefore can be used to save mycoplasma, gland substance, Chlamydia, and Positive rate is higher than traditional Hank's liquid.
2, sample culture
Test method: mycoplasma, gland substance, Chlamydia are acquired respectively with sampling swab, each sample is added separately to Formula 1, formula 2, formula 3 and formula 4 it is corresponding virus save liquid in saved (storage temperature is respectively: room temperature, 4 DEG C ,- 20 DEG C, -70 DEG C), and respectively 4h after being saved, 12h, 48h, 72h, 30d, 90d, 180d, 240d, 360d is inoculated into the chick embryo allantoic cavity of 10 ages in days (note: the sample under freezing needs first to thaw), observes 4d, record observation As a result.
It is following (positive+, negative -) to record result:
(1) mycoplasma
A, the corresponding virus of formula 1 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
B, the corresponding virus of formula 2 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
C, the corresponding virus of formula 3 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
D, the corresponding virus of formula 4 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
(2) gland substance
A, the corresponding virus of formula 1 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
B, the corresponding virus of formula 2 saves liquid
C, the corresponding virus of formula 3 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
D, the corresponding virus of formula 4 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
(3) Chlamydia
A, the corresponding virus of formula 1 saves liquid
B, the corresponding virus of formula 2 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
C, the corresponding virus of formula 3 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
D, the corresponding virus of formula 4 saves liquid
4h 12h 48h 72h 30d 90d 180d 240d 360d
Room temperature + + + - - - - - -
4℃ + + + - - - - - -
-20℃ + + + + + + + - -
-70℃ + + + + + + + + +
It can be seen that virus provided by the invention saves liquid can effectively save mycoplasma, gland substance, clothing original for a long time Body, therefore can be used to save mycoplasma, gland substance, Chlamydia.
In conclusion virus provided by the invention, which saves liquid, can not only be used to save clinical influenza virus, avian flu Malicious (such as H7N9), Sa Qi viral (A group), measles virus equal samples, can also be used to save mycoplasma, urea substance, Chlamydia etc. Sample, the scope of application is than wide, and the holding time is long, preservation effect is good, has good application value.
It should be noted that the above embodiments do not limit the invention in any form, it is all to use equivalent replacement or equivalent change The mode changed technical solution obtained, falls within the scope of protection of the present invention.

Claims (9)

1. the virus that one kind can effectively save viral equal samples for a long time saves liquid, which is characterized in that the virus saves liquid It consists of the following compositions:
Calcium chloride 0.1-3.5g, potassium chloride 0.07-1.49g, sodium chloride 0.76-2.76g, magnesium chloride 0.01-0.91g, magnesium sulfate 0.01-1.93g, sugar 0.1-0.361g, potassium dihydrogen phosphate 0.01-0.72g, disodium hydrogen phosphate 0.05-0.12g, sodium bicarbonate 0.007-0.67g, bovine serum albumin(BSA) 0.1-1g, antibiotic 0.6-5.3g, 4- hydroxyethyl piperazineethanesulfonic acid 0.236-0.96g, Phenol red 0.01-1g, L-cysteine hydrochloride 0.01-3.52g, Pidolidone 0.28-2.95g, water are settled to 100mL, pH value It is adjusted to 7.2-7.8.
2. the virus according to claim 1 that can effectively save viral equal samples for a long time saves liquid, which is characterized in that The sugar is the mixing of any one or two kinds in disaccharide and polysaccharide.
3. the virus according to claim 2 that can effectively save viral equal samples for a long time saves liquid, which is characterized in that What the disaccharide was selected is sucrose.
4. the virus according to claim 2 that can effectively save viral equal samples for a long time saves liquid, which is characterized in that What the polysaccharide was selected is glycogen.
5. the virus according to claim 1 that can effectively save viral equal samples for a long time saves liquid, which is characterized in that The antibiotic includes fungal antibiotic and bacteria antibiotic.
6. the virus according to claim 5 that can effectively save viral equal samples for a long time saves liquid, which is characterized in that The fungal antibiotic is the mixing of any one or two kinds in amphotericin B and nystatin.
7. the virus according to claim 5 that can effectively save viral equal samples for a long time saves liquid, which is characterized in that The bacteria antibiotic is any one or any several mixing in vancomycin, colistin and gentamicin.
8. the virus according to claim 1 that can effectively save viral equal samples for a long time saves liquid, which is characterized in that The sample includes virus, mycoplasma, gland substance and Chlamydia.
9. the virus according to claim 8 that can effectively save viral equal samples for a long time saves liquid, which is characterized in that The virus includes: influenza virus, Sa Qi virus A group and measles virus.
CN201910611068.6A 2019-07-08 2019-07-08 A kind of virus preservation liquid that can effectively save viral equal samples for a long time Pending CN110438089A (en)

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Cited By (15)

* Cited by examiner, † Cited by third party
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CN111518775A (en) * 2020-06-18 2020-08-11 合肥铼科生物科技有限公司 Preserving fluid for preserving virus sample at normal temperature for long time and application thereof
CN111690642A (en) * 2020-07-14 2020-09-22 福建百斯特基因检测有限公司 Virus sampling liquid composition and sampling method
CN111778239A (en) * 2020-07-14 2020-10-16 福建百斯特基因检测有限公司 Veterinary sampling liquid composition and sampling method
CN111808827A (en) * 2020-08-12 2020-10-23 泰州鑫联诚润生物技术有限公司 Virus preserving fluid capable of effectively preserving samples such as viruses for long time
CN111850090A (en) * 2020-08-05 2020-10-30 珠海市丽拓生物科技有限公司 Virus preserving fluid for effectively preserving virus sample
CN111869658A (en) * 2020-08-22 2020-11-03 南京健邦锦源医疗科技有限公司 Inactivated virus preservation solution and preparation method thereof
CN112481353A (en) * 2021-01-25 2021-03-12 青岛汉唐生物科技有限公司 Bacteriostatic virus preserving fluid and preparation method thereof
CN112501253A (en) * 2021-01-25 2021-03-16 青岛汉唐生物科技有限公司 Virus preserving fluid with preserving capability and preparation method thereof
CN112626168A (en) * 2020-12-26 2021-04-09 杭州百迈生物股份有限公司 Reagent composition for preserving virus sample and preservation method
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CN113337572A (en) * 2021-06-23 2021-09-03 江苏沃兴生物科技有限公司 Formula and preparation method of virus preservation solution
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CN115029345A (en) * 2022-04-24 2022-09-09 上海科技大学 Nucleic acid detection kit based on CRISPR and application thereof
EP4031649A4 (en) * 2019-09-17 2023-09-20 Longhorn Vaccines and Diagnostics, LLC Multipurpose compositions for collecting and transporting biological material

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US11821029B2 (en) 2019-09-17 2023-11-21 Longhorn Vaccines And Diagnostics, Llc Multipurpose compositions for collecting and transporting biological material
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CN110938603A (en) * 2019-12-20 2020-03-31 赛莱克斯(深圳)科技有限公司 Universal virus sample preservation buffer solution and preparation method thereof
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CN111850090A (en) * 2020-08-05 2020-10-30 珠海市丽拓生物科技有限公司 Virus preserving fluid for effectively preserving virus sample
CN111808827A (en) * 2020-08-12 2020-10-23 泰州鑫联诚润生物技术有限公司 Virus preserving fluid capable of effectively preserving samples such as viruses for long time
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CN112626168A (en) * 2020-12-26 2021-04-09 杭州百迈生物股份有限公司 Reagent composition for preserving virus sample and preservation method
CN112501253A (en) * 2021-01-25 2021-03-16 青岛汉唐生物科技有限公司 Virus preserving fluid with preserving capability and preparation method thereof
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CN112779164A (en) * 2021-01-28 2021-05-11 苏州赛普生物科技有限公司 Virus transport culture medium
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