CN110432263A - Botanical pesticide molecule-nano-carrier complex and its preparation method and application - Google Patents
Botanical pesticide molecule-nano-carrier complex and its preparation method and application Download PDFInfo
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- CN110432263A CN110432263A CN201910618636.5A CN201910618636A CN110432263A CN 110432263 A CN110432263 A CN 110432263A CN 201910618636 A CN201910618636 A CN 201910618636A CN 110432263 A CN110432263 A CN 110432263A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
- A01N25/06—Aerosols
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
- A01N25/10—Macromolecular compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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Abstract
The present invention provides a kind of botanical pesticide molecule-nano-carrier complex and its preparation method and application, and the botanical pesticide molecule-nano-carrier complex is formed by botanical pesticide molecule and nano-carrier through hydrogen bond and hydrophobic effect；The nano-carrier is dendritic macromole and passes through amido functional group functionalization.The present invention combines nano-carrier and botanical pesticide molecule by hydrogen bond and hydrophobic effect, the partial size of botanical pesticide kuh-seng base molecule is greatly decreased, especially matrine molecular particle size can be decreased to 10nm from 800nm, promote its dispersibility and stability in aqueous solution, process for preparation is easy to operate, quick simultaneously, for variety classes botanical pesticide, its virulence can be improved to some extent, extends its lasting period.
Description
Technical field
The present invention relates to technological field of biochemistry more particularly to botanical pesticide molecule-nano-carrier complex and its
Preparation method and application.
Background technique
Chemical pesticide plays an important role in terms of pest and disease damage prevention and control, Guarantee Grain Production, but it is not scientific
Use also bring many negative effects to agricultural sustainable development.
After being applied successfully in microelectronics, semi-conductor industry, biomedicine field from nanotechnology, it is in agricultural
Field development is swift and violent, provides new Scientific Methodology for modern agriculture science, promotion traditional agriculture interdisciplinary field not
Disconnected development.Nanoparticle refers to ultra-fine grain of the partial size between 1-100nm, because of its unique three dimension scale, has small ruler
The features such as very little, large specific surface area, modifiability are strong, aqueous dispersion is good, adhesiveness is strong, photocatalytic degradation.Botanical pesticide
It is applied relatively extensively in organic agriculture system, but due to the limitation of its own insecticidal effect, quick-acting and lasting period are more traditional
Medicine of learning to farm is poor, this seriously restricts botanical pesticide further applying in pest control field.It is expected to using nano-carrier
Improve the insecticidal properties of botanical pesticide, improves pest preventive effect.Therefore, the evil of the botanical pesticide of nano-carrier mediation is established
Worm prevention and control system is particularly important, has positive effect to the development of pesticide reduction pest controlling, green prevention and control technology.
Summary of the invention
The present invention provides a kind of botanical pesticide molecule-nano-carrier complex and its preparation method and application, with specific
The nano-carrier and botanical pesticide molecule of structure are combined with non-covalent intermolecular interaction to overcome existing botanical pesticide
Virulence and the poor problem of lasting effect.
In a first aspect, the present invention provides a kind of botanical pesticide molecule-nano-carrier complex, the botanical pesticide
Molecule-nano-carrier complex is combined by botanical pesticide molecule and nano-carrier through hydrogen bond and hydrophobic effect；It is described to receive
Meter Zai Ti is dendritic macromole and passes through amido functional group functionalization.
The internal cavity structures and high density structure of functional groups of nano-carrier are conducive to the loading of botanical pesticide molecule,
It forms stable complex, while after amido functional group functionalization, improving contained substance and insect cuticle and cell membrane
Affinity promotes botanical pesticide molecule to penetrate insect cuticle barrier, into the intracellular of body cavity and each organ-tissue, into
And play the target of efficient insecticide.
Further, the nano-carrier is one of star-type polymer and/or fluorescence dendritic macromole or more
Kind；
The star-type polymer is preferably macromolecular shown in formula 1 or formula 5；The fluorescence dendritic macromole is preferably selected from
Macromolecular shown in formula 2- formula 4；Wherein 5 structure of formula 1- formula is as follows:
Further, the botanical pesticide molecule is one of matrine, pyrethrins and D- limonene or more
Kind, preferably matrine.
Further, the botanical pesticide molecule-nano-carrier complex is star-like poly- as shown in matrine and formula 1
Close the complex that object is formed；Or the botanical pesticide molecule-nano-carrier complex is star-like as shown in matrine and formula 5
The complex that polymer is formed.
Further, the nano-carrier and the botanical pesticide molecular mass ratio are 1:8~8:1；
Further, when the nano-carrier is and the botanical pesticide molecule is respectively macromolecular described in formula 1 and hardship
When joining alkali, the mass ratio is 1:1；
It is described when the nano-carrier and the botanical pesticide molecule are respectively macromolecular described in formula 5 and matrine
Mass ratio is 1:1.
Further, the complex diameter is less than 10nm.
Second aspect, the present invention provides a kind of preparation methods of botanical pesticide molecule-nano-carrier complex, use
In preparing above-mentioned botanical pesticide molecule-nano-carrier complex, the specific steps are as follows:
The plant source molecule is formed after contacting as a solution respectively with nano-carrier.
The third aspect, the present invention provide one kind and contain a effective amount of above-mentioned botanical pesticide molecule-nano-carrier complex
Agricultural chemical insecticide, complex effective quantity is preferably 4 grams/ha, compared to be used alone 4.98 grams of botanical pesticide/it is public
Just 20% is reduced.
The present invention provides above-mentioned botanical pesticide molecule-nano-carrier complex and preparation method thereof and agricultural insecticides
Application of the agent in terms of pesticide reduction pest controlling.
Further, specifically comprise the following steps: when being applied to pesticide reduction pest controlling
1) botanical pesticide is mixed to get after solution being respectively configured in the nano-carrier and the botanical pesticide molecule
Molecule-nano-carrier complex medical fluid；
2) the botanical pesticide molecule-nano-carrier complex medical fluid is applied to insect body surface.
Further, step 2) is specifically by dripping method, spray-on process or infusion method by the botanical pesticide molecule-
Nano-carrier complex medical fluid is applied to insect body surface.
Further, the dripping method is specially to be dripped with appropriately sized liquid-transfering gun, injection needle or miniaturised nebuliser
It is added to insect body surface.
In one preferred embodiment of the invention, when the botanical pesticide molecule-nano-carrier complex is by hardship
When nano-carrier shown in ginseng alkali and formula 1 is compounded to form, the complex is reducing pesticide molecule partial size, promotes pesticide virulence side
There are clear superiorities in face；Preferably, under this complex method, nano-carrier mass ratio shown in matrine and formula 1 is 1:1, this
Condition makes complex have better effect in terms of combination.
The present invention selects particular kind of dendroid nano-carrier to prepare botanical pesticide molecule-nano-carrier complex,
The partial size of matrine can be narrowed down to 10 nanometers from 800 nanometers hereinafter, being a huge progress, for promoting matrine
Effect and duration and to reduce its usage amount most important.
The present invention provides a kind of botanical pesticide molecule-nano-carrier complex and its preparation method and application, beneficial to effect
Fruit is as follows:
(1) the dendroid nano-carrier and botanical pesticide molecule of the invention by Jing Guo amido functional group functionalization passes through
Hydrogen bond and hydrophobic effect combine, and the partial size of botanical pesticide molecule is greatly decreased, especially can by matrine molecular particle size from
800nm is decreased to 10nm, increases its specific surface area, promotes its dispersibility and stability in aqueous solution, while improving plant
The virulence and lasting effect of source pesticide.
(2) use scope of the present invention is extensive, for variety classes botanical pesticide, can improve its poison to some extent
Power extends its lasting period.
(3) configuration process is easy to operate, quick, only need to mix well according to mass ratio is recommended and stand 15min, so that it may
Stable complex is formed for conventional spray operation, it is easy to spread and universal.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, below will to embodiment or
Attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is this
Some embodiments of invention without creative efforts, may be used also for those of ordinary skill in the art
To obtain other drawings based on these drawings.
Fig. 1 is botanical pesticide molecule-nano-carrier complex application schematic diagram that the embodiment of the present invention 1 provides；
Fig. 2 is nano-carrier, matrine and matrine in the embodiment of the present invention 2-nano-carrier complex Electronic Speculum detection knot
Fruit figure；Wherein, A is matrine electromicroscopic photograph, and B is nano-carrier electromicroscopic photograph, and C is matrine-nano-carrier complex Electronic Speculum
Photo；
Fig. 3 is matrine-nano-carrier complex identical titration calorimetry detection result that the embodiment of the present invention 2 provides；
Fig. 4 is matrine-nano-carrier complex structure schematic diagram that the embodiment of the present invention 2 provides；
Fig. 5 is to detect matrine-nano-carrier complex virulence by pest backboard drop in experimental example 1 of the present invention to become
The line chart of change；
Fig. 6 is that experimental example 2 of the present invention passes through spray-on process detection three kinds of matrine, pyrethrins and D- limonene plant sources
Pesticide molecule and nano-carrier form the line chart of control efficiency variation after complex.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is
A part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill people
Member's every other embodiment obtained without making creative work, shall fall within the protection scope of the present invention.
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Embodiment 1
The present embodiment provides a kind of botanical pesticide molecule-nano-carrier complexs, by botanical pesticide molecule and nanometer
Carrier is formed through hydrogen bond and hydrophobic effect；Nano-carrier is dendritic macromole and passes through amido functional group functionalization.Nanometer carries
Body can select macromolecular shown in formula 1- formula 5, and structure is conducive to the loading of botanical pesticide molecule, be formed stable compound
Body, while nano-carrier passes through hydrophilic functional group's functionalization, is more advantageous to botanical pesticide molecule and penetrates insect cuticle screen
Barrier promotes its insecticide efficiency into the intracellular of body cavity and each organ-tissue.
Preparation method can be to match nano-carrier and botanical pesticide molecule respectively according to the ratio of mass ratio 1:1
It is mixed after setting solution.In this manner, nano-carrier and botanical pesticide molecule can be with noncovalent interaction (hydrogen bonds
And hydrophobic effect) combine, the partial size of botanical pesticide molecule is greatly decreased, and specific surface area increases, and has preferably divide in water
Dissipate property and stability.
Fig. 1 is the application schematic diagram of botanical pesticide molecule-nano-carrier complex provided by the invention, such as Fig. 1 institute
Show, after obtaining botanical pesticide molecule-nano-carrier complex medical fluid by above-mentioned preparation method, can will be answered by spray-on process
Fit medical fluid is added to insect body surface.
Further, appropriately sized utensil, such as liquid-transfering gun, injection needle or small can also be used when applying medical fluid
Medical fluid is added drop-wise to insect body surface by type sprayer, can also directly impregnate insect.
Further, macromolecular structure shown in formula 1 and preparation method are detailed in the patent working of publication number CN108794710A
Example 1, macromolecular structure shown in formula 2- formula 4 and preparation method are detailed in the patent Example 5 of patent No. ZL201310095194.3,
Macromolecular structure preparation method shown in formula 5 is detailed in the patent Example 3 of patent No. ZL201310119837.3.
The present embodiment provides a kind of botanical pesticide molecule-nano-carrier complexs, by nano-carrier and botanical pesticide
Molecule is interacted by non-covalent intermolecular and is combined, and the partial size of botanical pesticide molecule is greatly decreased, increases its specific surface
Product, promotes its dispersibility and stability in aqueous solution, while promoting its virulence and lasting period.
Embodiment 2
A kind of preparation method of botanical pesticide molecule-nano-carrier complex medical fluid is present embodiments provided, with formula 1
Shown star-type polymer is nano-carrier, and preparation method is detailed in disclosed in the patent Example 1 of publication number CN108794710A
The preparation method of star-type polymer, matrine and sterile water are general commercial product, the specific steps are as follows:
(1) 0.5g nano-carrier is dissolved in 80g sterile water, is prepared into nano-carrier solution；0.5 g matrine is dissolved in
In 20g sterile water, it is prepared into kuh-seng aqueous slkali.
(2) two kinds of solution are mixed, oscillation comes into full contact with two kinds of substances, is stored at room temperature 15min.
(3) it takes matrine-nano-carrier complex solution, kuh-seng aqueous slkali, nano-carrier solution to do dynamic optical respectively to dissipate
Analysis and scanning electron microscope are penetrated, the variation of matrine-nano-carrier complex partial size is detected.
(4) the combination situation of identical titration calorimetry detection matrine and nano-carrier is utilized.
Fig. 2 is nano-carrier provided in this embodiment, matrine and matrine-nano-carrier complex Electronic Speculum detection knot
Fruit figure；Wherein, A is matrine electromicroscopic photograph, and B is nano-carrier electromicroscopic photograph, and C is matrine-nano-carrier complex Electronic Speculum
Photo, as shown in Fig. 2, matrine molecular particle size is in 800nm or so, nano-carrier partial size is in 100nm or so, and complex grain
Diameter is decreased to 10nm or so.
Fig. 3 is matrine provided in this embodiment-nano-carrier complex identical titration calorimetry detection as a result, result
It shows △ H=40.93KJ/mol (positive value), Δ G=-37.10KJ/mol (negative value) shows kuh-seng base molecule and nano-carrier
It is by acting on (hydrogen bond and hydrophobic effect) spontaneous combination between Non-covalent molecular.It is possible thereby to determine that matrine-nano-carrier is multiple
Fit structure is as shown in Figure 4.
Nano-carrier and matrine are combined to obtain matrine-nanometer load to act between Non-covalent molecular by the present embodiment
Composite, partial size are decreased to 10nm by 800nm, and specific surface area increases, thus have in aqueous solution preferably dispersibility and
Stability.
Experimental example 1
This experimental example detects it using matrine prepared by embodiment 2-nano-carrier complex medical fluid as experimental subjects
Compared to variation of the common plant source pesticide in terms of virulence, the specific steps are as follows:
(1) matrine-nano-carrier complex medical fluid is diluted 100 times, 300 times, 500 times and 800 with sterile water respectively
Times.
(2) processing of backboard drop is carried out, forms the droplet of 0.2 μ L and dropwise addition, glutinous with the microinjector of appropriate range
It is attached to black peach aphid backboard, once sticks 10 black peach aphids, is repeated 8 times.
(3) it is repeated respectively with matrine, nano-carrier, sterile water replacement matrine-nano-carrier complex medical fluid simultaneously
Step (1) and (2).
(4) 1d, 3d, 5d and 7d observe and record black peach aphid death condition after the processing of backboard drop, calculate the death rate.
Fig. 5 is that matrine-nano-carrier complex virulence variation line chart, such as Fig. 5 are detected by pest backboard drop
Shown, matrine-nano-carrier complex medical fluid virulence of each extension rate is above matrine medical fluid, and black peach aphid the 7th day lethal
Rate promotes 10%~17.5%, illustrates that the present invention is obviously improved compared to general botanical pesticide virulence.
Experimental example 2
This experimental example detects it using matrine prepared by embodiment 2-nano-carrier complex medical fluid as experimental subjects
Compared to botanical pesticide in virulence and the variation in terms of the lasting period, the specific steps are as follows:
(1) 0.3% matrine, 1.5% pyrethrins and 5%D- limonene are diluted 500 times with sterile water respectively, point
Not Jia Ru nano-carrier to final concentration of 0.001%, mix well, be stored at room temperature 15min, be prepared into matrine-nano-carrier
The medical fluid of complex, pyrethrins-nano-carrier complex and D- limonene complex, sterile water is as control.
(2) medicine of above-mentioned each species complex is used in celery greenhouse using ultra low volume sprayer 528B (grand auspicious board)
Liquid and sterile water carry out medicament sprinkling experiment, and per treatment includes 10 plants of celery seedlings, are repeated 3 times, every square meter spray value 83mL,
1d, 3d, 5d, 7d and 10d count the population quantity of Frankliniella occidentalis on celery after by spraying, calculate Revision insect recluced rate and medicament is anti-
Effect.
Fig. 6 is that this experimental example passes through spray-on process detection matrine, three kinds of botanical pesticides of pyrethrins and D- limonene point
Son and nano-carrier form the line chart of control efficiency variation after complex, as shown in fig. 6, matrine, pyrethrins and D- lemon
Lemon alkene is combining rear Revision insect recluced rate and preventive effect to be promoted with nano-carrier, wherein matrine was promoted in the 10th day preventive effect
14.5%, D- limonene promote 18.3% in the 5th day preventive effect, and preventive effect promotes 23.5% to pyrethrins on day 3, illustrate to use
The method of the invention sprinkling complex medical fluid can effectively improve the virulence of botanical pesticide, extend its lasting period.
It should be understood that the dosage to above-described embodiment agents useful for same or raw material carries out equal proportion expansion or diminution
Technical solution afterwards, it is substantially identical with above-described embodiment.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；To the greatest extent
Present invention has been described in detail with reference to the aforementioned embodiments for pipe, those skilled in the art should understand that: it is still
It is possible to modify the technical solutions described in the foregoing embodiments, or part of technical characteristic is equally replaced
It changes；And these are modified or replaceed, the essence for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution
Mind and range.
Claims (10)
1. a kind of botanical pesticide molecule-nano-carrier complex, it is characterised in that: the botanical pesticide molecule-nanometer carries
Composite is combined by botanical pesticide molecule and nano-carrier through hydrogen bond and hydrophobic effect；The nano-carrier is branch
Shape macromolecular and process amido functional group functionalization.
2. botanical pesticide molecule-nano-carrier complex according to claim 1, which is characterized in that the nanometer carries
Body is one of star-type polymer and/or fluorescence dendritic macromole or a variety of；
The star-type polymer is preferably macromolecular shown in formula 1 or formula 5；The fluorescence dendritic macromole is preferably selected from formula 2- formula
Macromolecular shown in 4；Wherein 5 structure of formula 1- formula is as follows:
Wherein, independently value 1~100 the n in formula 1 and formula 5.
3. botanical pesticide molecule-nano-carrier complex according to claim 1, which is characterized in that the plant source
Pesticide molecule is one of matrine, pyrethrins and D- limonene or a variety of, preferably matrine.
4. botanical pesticide molecule-nano-carrier complex according to claim 1, which is characterized in that the plant source
Pesticide molecule-nano-carrier complex is the complex that the star-type polymer as shown in matrine and formula 1 is formed；Or the plant source
Pesticide molecule-nano-carrier complex is the complex that the star-type polymer as shown in matrine and formula 5 is formed.
5. botanical pesticide molecule-nano-carrier complex according to any one of claims 1 to 4, which is characterized in that institute
It states nano-carrier and the botanical pesticide molecular mass ratio is 1:8~8:1.
6. botanical pesticide molecule-nano-carrier complex according to claim 5, which is characterized in that when the nanometer
When carrier and the botanical pesticide molecule are respectively macromolecular described in formula 1 and matrine, the mass ratio is 1:1；
When the nano-carrier and the botanical pesticide molecule are respectively macromolecular described in formula 5 and matrine, the quality
Than for 1:1.
7. described in any item botanical pesticide molecule-nano-carrier complexs according to claim 1~6, which is characterized in that institute
Complex diameter is stated less than 10nm.
8. the preparation method of any one of the claim 1~7 botanical pesticide molecule-nano-carrier complex, feature exist
In, comprising:
The botanical pesticide molecule is formed after contacting as a solution respectively with the nano-carrier.
9. a kind of agricultural chemical insecticide, which is characterized in that the agricultural chemical insecticide contains any one of a effective amount of claim 1~7
Botanical pesticide molecule-nano-carrier the complex；It is preferred that the complex effective quantity is 4 grams/ha.
10. any one of the claim 1-7 botanical pesticide molecule-nano-carrier complex or system according to any one of claims 8
The application of Preparation Method or agricultural chemical insecticide as claimed in claim 9 in terms of pesticide reduction pest controlling.
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| CN112450213A (en) * | 2020-12-02 | 2021-03-09 | 苏州推动者生物科技有限公司 | Nano-carrier-agricultural active agent compound and preparation and application thereof |
| CN115191428A (en) * | 2022-07-25 | 2022-10-18 | 西安交通大学 | Amphiphilic star-shaped polymer loaded pesticide preparation and preparation method thereof |
| CN115299445A (en) * | 2022-10-08 | 2022-11-08 | 云南省烟草公司昆明市公司 | Compound solution for improving induced disease resistance of lentinan to plants, preparation method and method thereof, and method for preventing and controlling viruses in plants in field |
| CN117178996A (en) * | 2023-11-03 | 2023-12-08 | 中国农业大学三亚研究院 | Multi-element nano-composite for preventing spodoptera frugiperda as well as preparation method and application thereof |
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| CN112450213A (en) * | 2020-12-02 | 2021-03-09 | 苏州推动者生物科技有限公司 | Nano-carrier-agricultural active agent compound and preparation and application thereof |
| CN115191428A (en) * | 2022-07-25 | 2022-10-18 | 西安交通大学 | Amphiphilic star-shaped polymer loaded pesticide preparation and preparation method thereof |
| CN115191428B (en) * | 2022-07-25 | 2023-08-22 | 西安交通大学 | Amphiphilic star-type polymer-loaded pesticide preparation and preparation method thereof |
| CN115299445A (en) * | 2022-10-08 | 2022-11-08 | 云南省烟草公司昆明市公司 | Compound solution for improving induced disease resistance of lentinan to plants, preparation method and method thereof, and method for preventing and controlling viruses in plants in field |
| CN115299445B (en) * | 2022-10-08 | 2023-02-03 | 云南省烟草公司昆明市公司 | Compound solution for improving induced disease resistance of lentinan to plants, preparation method and method thereof, and method for preventing and controlling viruses in plants in field |
| CN117178996A (en) * | 2023-11-03 | 2023-12-08 | 中国农业大学三亚研究院 | Multi-element nano-composite for preventing spodoptera frugiperda as well as preparation method and application thereof |
| CN117178996B (en) * | 2023-11-03 | 2024-02-23 | 中国农业大学三亚研究院 | Multi-element nano-composite for preventing spodoptera frugiperda as well as preparation method and application thereof |
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