CN110423823A - A kind of C. guichenoti DNA bar code sequence and its application - Google Patents
A kind of C. guichenoti DNA bar code sequence and its application Download PDFInfo
- Publication number
- CN110423823A CN110423823A CN201910674536.4A CN201910674536A CN110423823A CN 110423823 A CN110423823 A CN 110423823A CN 201910674536 A CN201910674536 A CN 201910674536A CN 110423823 A CN110423823 A CN 110423823A
- Authority
- CN
- China
- Prior art keywords
- guichenoti
- bar code
- dna
- dna bar
- code sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000033272 Coreius guichenoti Species 0.000 title claims abstract description 57
- 241000894007 species Species 0.000 claims abstract description 26
- 238000001514 detection method Methods 0.000 claims abstract description 10
- 241000251468 Actinopterygii Species 0.000 claims description 15
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 8
- 229910052802 copper Inorganic materials 0.000 claims description 8
- 239000010949 copper Substances 0.000 claims description 8
- 102000015884 Cytochrome c oxidase subunit I Human genes 0.000 claims description 4
- 108050004212 Cytochrome c oxidase subunit I Proteins 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 101150087323 COI gene Proteins 0.000 abstract description 9
- 108020004414 DNA Proteins 0.000 description 39
- 238000000034 method Methods 0.000 description 12
- 238000001962 electrophoresis Methods 0.000 description 9
- 239000012634 fragment Substances 0.000 description 8
- 238000012163 sequencing technique Methods 0.000 description 7
- 102100030878 Cytochrome c oxidase subunit 1 Human genes 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 238000012408 PCR amplification Methods 0.000 description 6
- 238000004925 denaturation Methods 0.000 description 6
- 230000036425 denaturation Effects 0.000 description 6
- 239000000499 gel Substances 0.000 description 6
- 230000002068 genetic effect Effects 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000011144 upstream manufacturing Methods 0.000 description 4
- 229920000936 Agarose Polymers 0.000 description 3
- 241000252206 Cypriniformes Species 0.000 description 3
- 238000009004 PCR Kit Methods 0.000 description 3
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 3
- 238000000246 agarose gel electrophoresis Methods 0.000 description 3
- 238000000137 annealing Methods 0.000 description 3
- 230000002457 bidirectional effect Effects 0.000 description 3
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 239000000470 constituent Substances 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 238000004043 dyeing Methods 0.000 description 3
- 235000019441 ethanol Nutrition 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 230000001617 migratory effect Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000005086 pumping Methods 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 239000000376 reactant Substances 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000692787 Coreius Species 0.000 description 2
- 241000252210 Cyprinidae Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- 241001269238 Data Species 0.000 description 1
- 108020005196 Mitochondrial DNA Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 241000594009 Phoxinus phoxinus Species 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes
Abstract
The invention discloses a kind of C. guichenoti DNA bar code sequence and its applications, belong to species identification technical field.The C. guichenoti DNA bar code is C. guichenoti COI gene, which can be used as the standard detection sequence of C. guichenoti DNA, effectively identify C. guichenoti endemic species.
Description
Technical field
The invention belongs to species identification technical fields, and in particular to a kind of C. guichenoti DNA bar code sequence and its application.
Background technique
Species identification is always the vital basic steps of research on taxology or even almost all creatures field.Cause
This, accurately just seems to species identification and classification and is even more important.Currently, various countries researcher has carried out from lake to ocean
The fish DNA bar code project of different geographic regions efficiently may be used research shows that DNA bar code has in terms of species identification
Row.A large amount of result of study, which is shown, accurately can carry out species to all kinds of animals with the DNA bar code that COI gene is label
Identification, can select COI gene as the standard bar code in various animal bar code datas library.
DNA bar code refer to can be represented in organism the species, it is having enough variations, standard, easily amplification and
Relatively short DNA fragmentation.DNA bar code technology is just as the commodity bar code used in retail business, by species
One or more genes be scanned, it is established that mutual corresponding relationship between DNA sequence dna and biological species, so as to fast
Speed accurately carries out species identification.The COI gene of mitochondrial DNA have moderate length, evolutionary rate it is moderate and rich in system into
The features such as row development information.Under study for action, can the COI genetic fragment preferably to species effectively expanded.Therefore, dynamic
In object species taxonomy and identification, COI gene has very big potentiality as bar code applications.
There is certain limitation using traditional form identification method, for example will receive species gender, stage of development, phenotype
The influence of the factors such as plasticity and cause it is incorrect identification etc..In actual operation, it can also encounter that specimen amount is less and sample
The problems such as this preservation is imperfect leads to incorrect identification.Traditional taxology method is combined using DNA bar code, to species
The identification and classification of efficiently and accurately play an important role.
C. guichenoti (Coreius guichenoti), be subordinate to Cyprinidae (Cyprinidae) , Minnow subfamily (Gobioninae),
Copper fish category (Coreius).It is distributed mainly on the Endemic fish of Upper Yangtze River, Jinsha jiang River middle and lower reaches and Yalongjiang River downstream.On the Changjiang river
The gradually construction and operation for swimming Heavenly Stems and Earthly Branches stream step hydropower station result in the migratory C. guichenoti habitat in river and are cut off, affect
The completion of the history of life, and inhabit ground area and also largely reduce.In the assessment of Chinese Wild vertebrate Endangered status, circle
Mouth copper fish is in pole danger grade and is therefore effectively identified C. guichenoti endemic species and species conservation has been very urgent.
In existing technology, without C. guichenoti DNA bar code standard detection sequence and C. guichenoti COI gene
Record and report as C. guichenoti DNA bar code standard detection sequence.
Summary of the invention
It is an object of the present invention to provide a kind of standard detection sequences that can be used as C. guichenoti DNA, can be effectively to circle
Mouth copper fish endemic species are identified.
The technical scheme adopted by the invention is that:
A kind of C. guichenoti DNA bar code sequence, sequence are SEQ ID NO.1, particular sequence are as follows:
Preferably, the C. guichenoti DNA bar code is C. guichenoti COI (cytochrome c oxidase
Subunit I, cytochrome c oxidase subunit I) gene.
The DNA bar code sequence can be used as the standard detection sequence of C. guichenoti DNA, effectively peculiar to C. guichenoti
Kind and its sample are identified.
The invention has the following advantages that
(1) the obtained C. guichenoti DNA bar code sequence of the present invention can be used as the standard detection sequence of C. guichenoti DNA
Column, to the progress and classification of C. guichenoti endemic species efficiently and accurately, while using the DNA bar code sequence to C. guichenoti sample
Product are identified that step is few, method is simple.
(2) in actual operation, which facilitates comparison convenient for saving.
(3) C. guichenoti is distributed mainly on the Endemic fish of Upper Yangtze River, Jinsha jiang River middle and lower reaches and Yalongjiang River downstream.The Changjiang river
The gradually construction and operation of upstream Heavenly Stems and Earthly Branches stream step hydropower station result in the migratory C. guichenoti habitat in river and are cut off, influence
The completion of the history of life, and inhabit ground area and also largely reduce.In the assessment of Chinese Wild vertebrate Endangered status,
C. guichenoti is in pole danger grade.Technical solution of the present invention can effectively identify C. guichenoti species, to instruct it
The protection of resource.
Specific embodiment
Embodiment 1
5 C. guichenoti samples of Jinsha jiang River downstream Burner zone reservoir area river Hui Xi section are taken to be verified.
The extraction of 1.DNA
The fish musculature of clip 5mg or so, shreds as far as possible, is placed in the centrifuge tube of 1.5ml, places at room temperature,
The pumping of genomic DNA is carried out using the cell/tissue genomic kit of GENEray company after ethyl alcohol volatilizees completely completely
It mentions.
2.PCR amplification
PCR primer uses COI gene in the universal primer of Cypriniformes fish, and upstream and downstream primer sequence is respectively as follows:
CypFCOI:TCTCAACCAACCACAAAGACATTGG, CypRCOI:GACTTCTGGGTGGCCAAAGAATCA.PCR reactant
Be total volume it is 50 μ l, PCR amplification is carried out using PCR kit, specific ingredient is shown in Table 1, PCR response procedures and is shown in Table 2.
1 PCR reaction system of table
| Reacted constituent | Volume (μ l) |
| Sterilize distilled water | 22 |
| CypFCOI | 1(10Μm/μl) |
| CypRCOI | 1(10Μm/μl) |
| Genomic DNA template | 1(20ng/μl) |
| 2ⅹPCR mix | 25 |
2 PCR response procedures of table
| Step | Temperature | Time |
| 1. initial denaturation | 94℃ | 5min |
| 2. denaturation | 94℃ | 30s |
| 3. annealing | 52℃ | 30s |
| 4. extending | 72℃ | 1min |
| 5.4to2 | Circulation 30 times | |
| 6. extending eventually | 72℃ | 10min |
3. agarose gel electrophoresis detects
It carries out carrying out electrophoresis to PCR product with the standard of constant pressure 5V/cm, when bromophenol blue is moved on to away from Ago-Gel forward position about
When 5cm, stop electrophoresis.Gel is taken out, is placed in EB dyeing liquor and impregnates 15min, then observed with ultraviolet transilluminator.
4. sequencing
Through agarose electrophoresis testing goal band clearly PCR product, carried out with centrifugation pillar PCR product purification kit
After the recovery purifying of target fragment, send to one Hui Yuan Biotechnology Co., Ltd of Wuhan Tian and carry out bidirectional sequencing
The COI genetic fragment of 1 C. guichenoti in embodiment 1 are as follows:
It is 99.6% with C. guichenoti DNA bar code sequence similarity degree by comparison, it can judge the species for circle
Mouth copper fish.
Embodiment 2
The extraction of 1.DNA
The fish musculature of clip 5mg or so, shreds as far as possible, is placed in the centrifuge tube of 1.5ml, places at room temperature,
The pumping of genomic DNA is carried out using the cell/tissue genomic kit of GENEray company after ethyl alcohol volatilizees completely completely
It mentions.
2.PCR amplification
PCR primer uses COI gene in the universal primer of Cypriniformes fish, and upstream and downstream primer sequence is respectively as follows:
CypFCOI:TCTCAACCAACCACAAAGACATTGG, CypRCOI:GACTTCTGGGTGGCCAAAGAATCA.PCR reactant
Be total volume it is 50 μ l, PCR amplification is carried out using PCR kit, specific ingredient is shown in Table 1, PCR response procedures and is shown in Table 2.
1 PCR reaction system of table
| Reacted constituent | Volume (μ l) |
| Sterilize distilled water | 22 |
| CypFCOI | 1(10Μm/μl) |
| CypRCOI | 1(10Μm/μl) |
| Genomic DNA template | 1(20ng/μl) |
| 2ⅹPCR mix | 25 |
2 PCR response procedures of table
| Step | Temperature | Time |
| 1. initial denaturation | 94℃ | 5min |
| 2. denaturation | 94℃ | 30s |
| 3. annealing | 52℃ | 30s |
| 4. extending | 72℃ | 1min |
| 5.4to2 | Circulation 30 times | |
| 6. extending eventually | 72℃ | 10min |
3. agarose gel electrophoresis detects
It carries out carrying out electrophoresis to PCR product with the standard of constant pressure 5V/cm, when bromophenol blue is moved on to away from Ago-Gel forward position about
When 5cm, stop electrophoresis.Gel is taken out, is placed in EB dyeing liquor and impregnates 15min, then observed with ultraviolet transilluminator.
4. sequencing
Through agarose electrophoresis testing goal band clearly PCR product, carried out with centrifugation pillar PCR product purification kit
After the recovery purifying of target fragment, send to one Hui Yuan Biotechnology Co., Ltd of Wuhan Tian and carry out bidirectional sequencing
The COI genetic fragment of 1 C. guichenoti in embodiment 2 are as follows:
It is 99.6% with C. guichenoti DNA bar code sequence similarity degree by comparison, it can judge the species for circle
Mouth copper fish.
Embodiment 3
The extraction of 1.DNA
The fish musculature of clip 5mg or so, shreds as far as possible, is placed in the centrifuge tube of 1.5ml, places at room temperature,
The pumping of genomic DNA is carried out using the cell/tissue genomic kit of GENEray company after ethyl alcohol volatilizees completely completely
It mentions.
2.PCR amplification
PCR primer uses COI gene in the universal primer of Cypriniformes fish, and upstream and downstream primer sequence is respectively as follows:
CypFCOI:TCTCAACCAACCACAAAGACATTGG, CypRCOI:GACTTCTGGGTGGCCAAAGAATCA.PCR reactant
Be total volume it is 50 μ l, PCR amplification is carried out using PCR kit, specific ingredient is shown in Table 1, PCR response procedures and is shown in Table 2.
1 PCR reaction system of table
| Reacted constituent | Volume (μ l) |
| Sterilize distilled water | 22 |
| CypFCOI | 1(10Μm/μl) |
| CypRCOI | 1(10Μm/μl) |
| Genomic DNA template | 1(20ng/μl) |
| 2ⅹPCR mix | 25 |
2 PCR response procedures of table
| Step | Temperature | Time |
| 1. initial denaturation | 94℃ | 5min |
| 2. denaturation | 94℃ | 30s |
| 3. annealing | 52℃ | 30s |
| 4. extending | 72℃ | 1min |
| 5.4to2 | Circulation 30 times | |
| 6. extending eventually | 72℃ | 10min |
3. agarose gel electrophoresis detects
It carries out carrying out electrophoresis to PCR product with the standard of constant pressure 5V/cm, when bromophenol blue is moved on to away from Ago-Gel forward position about
When 5cm, stop electrophoresis.Gel is taken out, is placed in EB dyeing liquor and impregnates 15min, then observed with ultraviolet transilluminator.
4. sequencing
Through agarose electrophoresis testing goal band clearly PCR product, carried out with centrifugation pillar PCR product purification kit
After the recovery purifying of target fragment, send to one Hui Yuan Biotechnology Co., Ltd of Wuhan Tian and carry out bidirectional sequencing
The COI genetic fragment of 1 C. guichenoti in embodiment 3 are as follows:
It is 99.7% with C. guichenoti DNA bar code sequence similarity degree by comparison, it can judge the species for circle
Mouth copper fish.
It measures in 5 samples simultaneously, in addition the COI genetic fragment Yu C. guichenoti DNA bar code of 2 C. guichenoti samples
Standard detection sequence similarity is 100%, it can judges the species for C. guichenoti.
According to sequencing result, the nucleotide sequence of above 5 samples and C. guichenoti DNA bar code standard detection sequence
Homology can determine whether that these species are C. guichenoti 98% or more.
Embodiment 1, embodiment 2 and embodiment 3PCR reaction system and PCR response procedures are all the same, in this way can be more
The accuracy for the C. guichenoti DNA bar code sequence that objectively the confirmation present invention obtains.
The obtained C. guichenoti DNA bar code sequence of the present invention can be used as the standard detection sequence of C. guichenoti DNA,
To the progress and classification of C. guichenoti endemic species efficiently and accurately, while using the DNA bar code sequence to C. guichenoti sample
It is identified, step is few, method is simple.In actual operation, which facilitates comparison convenient for saving.Circle
Mouth copper fish is distributed mainly on the Endemic fish of Upper Yangtze River, Jinsha jiang River middle and lower reaches and Yalongjiang River downstream.Hydro-electricity ladder
The gradually construction and operation in grade power station result in the migratory C. guichenoti habitat in river and are cut off, affect the complete of the history of life
At, and inhabit ground area and also largely reduce.In the assessment of Chinese Wild vertebrate Endangered status, C. guichenoti is in
Pole danger grade.Technical solution of the present invention can effectively identify C. guichenoti species, to instruct the protection of its resource.
It should be noted last that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although ginseng
It is described the invention in detail according to preferred embodiment, those skilled in the art should understand that, it can be to the present invention
Technical solution be modified or replaced equivalently, without departing from the spirit and scope of the technical solution of the present invention, should all cover
In the scope of the claims of the present invention.
Sequence table
<110>Jianghan University
<120>a kind of C. guichenoti DNA bar code sequence and its application
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 672
<212> DNA
<213>C. guichenoti (Coreius guichenotiDNA bar code sequence)
<400> 1
gacattggca ccctttatct tgtatttggt gcctgagccg gcatagtagg gactgcttta 60
agcctcctca ttcgagctga actaagccag cccggatcac tactaggtga tgatcaaatt 120
tacaatgtta tcgttactgc ccacgccttc gtaataattt tctttatagt aataccaatc 180
cttattggcg gatttggaaa ctgactcgta ccgctaataa ttggagcacc cgatatggca 240
ttcccacgaa taaataatat aagtttctga cttttgccac cctcattcct tctattacta 300
gcctcttccg gggttgaagc tggggctggg acaggatgaa cagtttaccc accacttgca 360
ggtaatcttg cccatgcagg agcatcagta gacctaacaa ttttttcact gcacctagca 420
ggtgtctcat caatcttagg ggcaattaac ttcatcacca caaccattaa tatgaaaccc 480
ccagctattt cccaatacca aacacccctc tttgtgtggg ccgtacttgt aacagctgta 540
cttctccttc tatcactacc agtcttagct gccggaatta caatgcttct tacagaccgt 600
aatcttaata ccacattctt tgacccagca gggggaggag acccaatttt gtatcaacac 660
ttattctgat tc 672
Claims (3)
1. a kind of C. guichenoti DNA bar code sequence, which is characterized in that sequence is SEQ ID NO.1.
2. C. guichenoti DNA bar code sequence according to claim 1, which is characterized in that the C. guichenoti DNA bar shaped
Code is C. guichenoti COI (cytochrome c oxidase subunit I, cytochrome c oxidase subunit I) gene.
3. a kind of application of C. guichenoti DNA bar code sequence, which is characterized in that the DNA bar code sequence can be used as round mouth
The standard detection sequence of copper fish DNA, effectively identifies C. guichenoti endemic species and its sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910674536.4A CN110423823A (en) | 2019-07-25 | 2019-07-25 | A kind of C. guichenoti DNA bar code sequence and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910674536.4A CN110423823A (en) | 2019-07-25 | 2019-07-25 | A kind of C. guichenoti DNA bar code sequence and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110423823A true CN110423823A (en) | 2019-11-08 |
Family
ID=68412308
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910674536.4A Pending CN110423823A (en) | 2019-07-25 | 2019-07-25 | A kind of C. guichenoti DNA bar code sequence and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110423823A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114941034A (en) * | 2022-05-27 | 2022-08-26 | 中国长江三峡集团有限公司中华鲟研究所 | COI primer pair, kit and identification method for identifying cupfish and cupfish |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105063031A (en) * | 2015-08-05 | 2015-11-18 | 中国长江三峡集团公司 | Coreius guichenoti microsatellite markers and use thereof |
| CN108998547A (en) * | 2018-09-18 | 2018-12-14 | 中国水产科学研究院长江水产研究所 | A kind of microsatellite marking method for C. guichenoti paternity test |
-
2019
- 2019-07-25 CN CN201910674536.4A patent/CN110423823A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105063031A (en) * | 2015-08-05 | 2015-11-18 | 中国长江三峡集团公司 | Coreius guichenoti microsatellite markers and use thereof |
| CN108998547A (en) * | 2018-09-18 | 2018-12-14 | 中国水产科学研究院长江水产研究所 | A kind of microsatellite marking method for C. guichenoti paternity test |
Non-Patent Citations (2)
| Title |
|---|
| FEI CHENG等: "Population genetic structure and its implication for conservation of Coreius guichenoti in the upper Yangtze River", 《ENVIRONMENTAL BIOLOGY OF FISHES》 * |
| 黄燕: "长江上游特有鱼类DNA条形码研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114941034A (en) * | 2022-05-27 | 2022-08-26 | 中国长江三峡集团有限公司中华鲟研究所 | COI primer pair, kit and identification method for identifying cupfish and cupfish |
| CN114941034B (en) * | 2022-05-27 | 2023-10-13 | 中国长江三峡集团有限公司中华鲟研究所 | COI primer pair, kit and identification method for identifying copper fish with round mouth |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN114686597A (en) | SNP molecular marker for sex identification of salangid and application thereof | |
| CN105969882B (en) | One kind haplotype SNP marker relevant to channel catfish fast-growth and its detection method and application | |
| Adams et al. | Genomic investigation of the strawberry pathogen Phytophthora fragariae indicates pathogenicity is associated with transcriptional variation in three key races | |
| CN109022457A (en) | A kind of * DNA bar code sequence and its application | |
| CN109055401A (en) | A kind of widow's squama floats fish DNA bar code sequence and its application | |
| CN102382878B (en) | Molecular marking method for discriminating different family of Cyprinus carpiovar jian | |
| CN110423823A (en) | A kind of C. guichenoti DNA bar code sequence and its application | |
| CN109207620A (en) | A kind of precious magnificent carpinus turczaninowii ISSR-PCR molecular labeling system | |
| CN102321750A (en) | Method for rapidly screening bian chicken weight gain degree by molecular marking | |
| CN110541035A (en) | Sinocyclocheilus sinensis DNA barcode sequence and application thereof | |
| CN110438241A (en) | A kind of opsariichthys bidens DNA bar code sequence and its application | |
| CN104004750A (en) | Eriocheir sinensis sexual precocity property related SNP marker and its application | |
| CN104099423B (en) | For the molecular labeling of cutter long-tailed anchovy different ecological type population identification | |
| CN111944917A (en) | Method for developing camellia plant SSR primers based on transcriptome sequencing | |
| CN109055569A (en) | A kind of Pelteobagrus fulvidraco DNA bar code sequence and its application | |
| CN109055400A (en) | A kind of Usu intend Chang DNA bar code sequence and its application | |
| CN109055407A (en) | A kind of erythroculter ilishaeformis DNA bar code sequence and its application | |
| CN109055403A (en) | A kind of Pelteobagrus vachelli DNA bar code sequence and its application | |
| CN109988848B (en) | Primer, kit and method for identifying ectropis minutissima | |
| CN104073562B (en) | A kind of molecular marker for cutter long-tailed anchovy different ecological type population identification | |
| CN107841563A (en) | Differentiate the molecular specificity labeled primers and method of catfish | |
| Aprilianingsih et al. | DNA Barcode of Homalomena pexa inferred from Internal Transcribed Spacer region | |
| CN110423824A (en) | A kind of She Minnow DNA bar code sequence and its application | |
| CN112522422A (en) | Molecular identification method of pelagic fish and little-scale pelagic fish based on COI gene fragment | |
| CN109055405A (en) | A kind of red fin Culter DNA bar code sequence and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191108 |