CN110419621A - A kind of preparation method of high gel type whey separated protein powder - Google Patents

A kind of preparation method of high gel type whey separated protein powder Download PDF

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Publication number
CN110419621A
CN110419621A CN201910820265.9A CN201910820265A CN110419621A CN 110419621 A CN110419621 A CN 110419621A CN 201910820265 A CN201910820265 A CN 201910820265A CN 110419621 A CN110419621 A CN 110419621A
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China
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whey
powder
freeze
gel type
protein isolate
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CN201910820265.9A
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Chinese (zh)
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胥伟
蒋盼盼
王斌
王宏勋
易阳
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Wuhan Polytechnic University
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Wuhan Polytechnic University
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Priority to CN201910820265.9A priority Critical patent/CN110419621A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00Working-up of proteins for foodstuffs
    • A23J3/04Animal proteins
    • A23J3/08Dairy proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Dairy Products (AREA)

Abstract

The present invention discloses a kind of preparation method of high gel type whey separated protein powder, is related to food processing technology field.The high gel type whey separated protein powder obtains extruding powder the following steps are included: extrusion processing after whey protein isolate freeze-dried powder is mixed with water;After the extruding powder freeze-drying process, high gel type whey separated protein powder is crushed to obtain.The present invention adjusts the water content of whey separated protein powder by mixing with water, and then is handled by extrusion, makes gelation that is protein-modified in whey protein isolate, and then improving whey protein isolate using high temperature, high pressure, high shear forces.The method of the present invention compares currently used physics, chemistry, biological modified method, will not introduce foreign aid's material, highly-safe, has many advantages, such as that the operating time is short, at low cost, modified effect is good, can be used for industrialized production.

Description

A kind of preparation method of high gel type whey separated protein powder
Technical field
The present invention relates to food processing technology field, in particular to a kind of preparation side of high gel type whey separated protein powder Method.
Background technique
Whey protein isolate is the main component of whey, and amino acid composition is reasonable, digestibility is high, is had good Nutritive peculiarity and higher nutritive value are important one of the high-quality proteins of the mankind, are widely used in food industry.With cream The development of process for manufacturing product and whey protein isolate self-characteristic, whey protein isolate are widely used in adjustment gel The institutional framework of food.Nevertheless, causing it to eat gel since the gel characteristic of whey protein isolate itself is incomplete The adjustment effect of product institutional framework is limited, to limit the application range of whey protein isolate.Therefore, in recent years, both at home and abroad Researcher is devoted to the study on the modification of whey protein isolate to enhance the gel characteristic of whey protein isolate.
Currently, common method of modifying includes: heat treatment, microwave treatment, chemical modification processing method and enzyme modification Deng.Wherein, heat treatment method modified effect is poor;The higher cost of microwave handling method and enzyme modification method, is unfavorable for work Industry metaplasia produces;Not only the operating time is long for chemical modification processing method, but also is readily incorporated allogenic material and safety is poor.
Summary of the invention
The main object of the present invention is to propose a kind of preparation method of high gel type whey separated protein powder, it is intended to solve mesh The problem that preceding whey protein isolate method of modifying is at high cost, the operating time is long.
To achieve the above object, the present invention proposes a kind of preparation method of high gel type whey separated protein powder, the height The preparation method of gel-type whey separated protein powder the following steps are included:
Extrusion is handled after whey protein isolate freeze-dried powder is mixed with water, obtains extruding powder;
After the extruding powder freeze-drying process, high gel type whey separated protein powder is crushed to obtain.
Optionally, it is described whey protein isolate freeze-dried powder is mixed with water after extrusion handle, the step of powder must be squeezed In, the weight ratio of the whey protein isolate freeze-dried powder and water is 100:(3~7).
Optionally, which is characterized in that the weight ratio of the whey protein isolate freeze-dried powder and water is 100:5.
Optionally, it is described whey protein isolate freeze-dried powder is mixed with water after extrusion handle, the step of powder must be squeezed In, the technological parameter of the extrusion are as follows: 90~130rpm of screw speed, 35~50 DEG C of barrel temperature, die head length 25~ 30mm。
Optionally, the technological parameter of the extrusion are as follows: screw speed 100rpm, 45 DEG C of barrel temperature, die head length 25mm。
Optionally, it is described whey protein isolate freeze-dried powder is mixed with water after extrusion handle, the step of powder must be squeezed Before further include:
Whey separated protein powder is dissolved in water, forms whey protein isolate solution;
Under 73~92MPa, to the whey protein isolate solution homogenization;
By the whey protein isolate solution freeze-drying after homogenization, whey protein isolate freeze-dried powder is obtained.
Optionally, in described the step of whey separated protein powder is dissolved in water, forms whey protein isolate solution, the cream Mass concentration of the sorting from protein solution is 7%~9%.
Optionally, be set forth under 73~92MPa, the step of to the whey protein isolate solution homogenization in, it is described The time of homogenization is 2.5~4min.
Optionally, described that high gel type whey separated protein powder after the extruding powder freeze-drying process, will be crushed to obtain In step, the partial size of the high gel type whey separated protein powder is not more than 80 mesh.
In technical solution provided by the invention, extrusion is handled after whey protein isolate freeze-dried powder is mixed with water, is led to It crosses and is mixed with water to adjust the water content of whey separated protein powder, and then handled by extrusion, utilize high temperature, high pressure, height Shear action makes gelation that is protein-modified in whey protein isolate, and then improving whey protein isolate.Side of the present invention Method compares currently used physics, chemistry, biological modified method, will not introduce foreign aid's material, highly-safe, has the operating time Short, at low cost, the advantages that modified effect is good, it can be used for industrialized production.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with Other relevant attached drawings are obtained according to these attached drawings.
Fig. 1 is the process signal of an embodiment of the preparation method of high gel type whey separated protein powder proposed by the present invention Figure;
Fig. 2 is that the process of another embodiment of the preparation method of high gel type whey separated protein powder proposed by the present invention is shown It is intended to.
The embodiments will be further described with reference to the accompanying drawings for the realization, the function and the advantages of the object of the present invention.
Specific embodiment
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention Technical solution be clearly and completely described.Obviously, described embodiment is only a part of the embodiments of the present invention, Instead of all the embodiments.
It should be noted that the person that is not specified actual conditions in embodiment, according to conventional conditions or manufacturer's recommended conditions It carries out.Reagents or instruments used without specified manufacturer is the conventional products that can be obtained by commercially available purchase.In addition, The meaning of the "and/or" occurred in full text, including three schemes arranged side by side, by taking " A and/or B " as an example, including A scheme or the side B The scheme that case or A and B meet simultaneously.In addition, the technical solution between each embodiment can be combined with each other, but must be Based on can be realized by those of ordinary skill in the art, answered when conflicting or cannot achieve when occurs in the combination of technical solution When the combination for thinking this technical solution is not present, also not the present invention claims protection scope within.Based in the present invention Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all Belong to the scope of protection of the invention.
Since the gel characteristic of whey protein isolate itself is incomplete, lead to its adjustment to gel food institutional framework Effect is limited, to limit the application range of whey protein isolate.Therefore, in recent years, domestic and international researcher is devoted to The study on the modification of whey protein isolate is to enhance the gel characteristic of whey protein isolate.Currently, common method of modifying includes: heat Processing, microwave treatment, chemical modification processing method and enzyme modification etc..Wherein, heat treatment method modified effect is poor;Microwave The higher cost of processing method and enzyme modification method, is unfavorable for industrialized production;When chemical modification processing method not only operates Between it is long, and be readily incorporated allogenic material and safety is poor.
In consideration of it, the present invention proposes a kind of preparation method of high gel type whey separated protein powder, Fig. 1 is the preparation method An embodiment.After preparation method of the present invention mixes whey protein isolate freeze-dried powder with water extrusion handle, by with water It mixes to adjust the water content of whey separated protein powder, and then handled by extrusion, is made using high temperature, high pressure, high shear With making gelation that is protein-modified in whey protein isolate, and then improving whey protein isolate.It compares above-mentioned common Physics, chemistry, biological modified method, preparation method of the present invention will not introduce foreign aid's material, highly-safe, have the operating time Short, at low cost, the advantages that modified effect is good, it can be used for industrialized production.
Refering to fig. 1, in the present embodiment, the preparation method of the high gel type whey separated protein powder the following steps are included:
Step S10, extrusion is handled after mixing whey protein isolate freeze-dried powder with water, obtains extruding powder.
Step S20, by after the extruding powder freeze-drying process, high gel type whey separated protein powder is crushed to obtain.
In the present embodiment, the mixed proportion of whey protein isolate freeze-dried powder and water can be preferably 100:(3~7).It is i.e. every 3~7 parts of water are mixed in 100 parts of whey protein isolate freeze-dried powder, and the water content of whey protein isolate freeze-dried powder is adjusted with this, And then optimize the promotion effect of the gel characteristic of whey protein isolate after extrusion is handled.Further, whey protein isolate The preferred 100:5 of the weight ratio of freeze-dried powder and water.
After whey protein isolate freeze-dried powder and water are sufficiently mixed, will can squeeze in mixture feeding extruder swollen Change processing, wherein extruder can be twin (double) screw extruder.In extrusion pressing puffing process, protein is in high temperature, high pressure and high shear Equal many conditions coupling issues changing property, and the gel hardness and water-retaining property of protein obviously increase.In extrusion pressing puffing process The technological parameters such as screw speed, barrel temperature and die head length will have a direct impact on the gel characteristic of product, in consideration of it, this reality It applies example and parameter optimization, the technological parameter of the extrusion are as follows: screw rod is carried out to screw speed, barrel temperature and die head length 90~130rpm of revolving speed, 35~50 DEG C of barrel temperature, 25~30mm of die head length.Further, the technological parameter of extrusion It is preferred that are as follows: screw speed 100rpm, 45 DEG C of barrel temperature, die head length 25mm.
In addition, the present embodiment to through extrusion treated squeeze powder freeze-drying process, and pulverize and sieve with obtain The finished product of high gel type whey separated protein powder, wherein when pulverizing and sieving, using 80 meshes, so that high gel type whey The partial size of separated protein powder is not more than 80 mesh.It should be noted that above-mentioned 80 mesh refers to that mesh number is the normal test sieve of 80 mesh, According to the corresponding relationship of sieve model and pore size as defined in Chinese Pharmacopoeia, the present embodiment high gel type whey separated protein powder Partial size be not more than 0.173mm.
In addition, based on the above embodiment, the present invention proposes another embodiment, Fig. 2 is the flow diagram of another embodiment. Referring to Fig.2, the present embodiment preparation method the following steps are included:
Step S100, whey separated protein powder is dissolved in water, forms whey protein isolate solution.
Wherein, the amount of taking of whey separated protein powder and water is subject to the concentration of whey protein isolate solution, the present embodiment In, the mass concentration of the whey protein isolate solution is 7 %~9 (w/w) (w/w) %.
Step S200, under 73~92MPa, to the whey protein isolate solution homogenization.
Wherein, the time of homogenization is 2.5~4min.
Step S300, the whey protein isolate solution after homogenization is lyophilized, obtains whey protein isolate freeze-dried powder.
Step S10, extrusion is handled after mixing whey protein isolate freeze-dried powder with water, obtains extruding powder.
Step S20, by after the extruding powder freeze-drying process, high gel type whey separated protein powder is crushed to obtain.
The present embodiment is combined high-pressure homogeneous with extruding and puffing technology, and first passing through high-pressure homogeneous processing makes whey protein isolate Molecular moiety expansion makes hydrophobic grouping exposure, makes to interact between protein molecule, so that gel hardness and elasticity are improved, Extrusion is handled again, further enhances gel characteristic.
Technical solution of the present invention is described in further detail below in conjunction with specific embodiments and the drawings, it should be understood that Following embodiment is only used to explain the present invention, is not intended to limit the present invention.
Embodiment 1
After whey protein isolate freeze-dried powder is mixed with water by weight 100:5, it is sent into twin (double) screw extruder, and set The screw speed of twin (double) screw extruder is 100rpm, 45 DEG C of barrel temperature, die head length are 25mm.After to be extruded, it will squeeze Press-powder takes out and is freeze-dried, then crushes and cross 80 meshes, and high gel type whey separated protein powder can be obtained.
Embodiment 2
After whey protein isolate freeze-dried powder is mixed with water by weight 100:3, it is sent into twin (double) screw extruder, and set The screw speed of twin (double) screw extruder is 130rpm, 47 DEG C of barrel temperature, die head length are 28mm.After to be extruded, it will squeeze Press-powder takes out and is freeze-dried, then crushes and cross 80 meshes, and high gel type whey separated protein powder can be obtained.
Embodiment 3
After whey protein isolate freeze-dried powder is mixed with water by weight 100:7, it is sent into twin (double) screw extruder, and set The screw speed of twin (double) screw extruder is 90rpm, 50 DEG C of barrel temperature, die head length are 30mm.After to be extruded, it will squeeze Powder takes out and is freeze-dried, then crushes and cross 80 meshes, and high gel type whey separated protein powder can be obtained.
Embodiment 4
After whey protein isolate freeze-dried powder is mixed with water by weight 100:6, it is sent into twin (double) screw extruder, and set The screw speed of twin (double) screw extruder is 110rpm, 35 DEG C of barrel temperature, die head length are 26mm.After to be extruded, it will squeeze Press-powder takes out and is freeze-dried, then crushes and cross 80 meshes, and high gel type whey separated protein powder can be obtained.
Embodiment 5
It takes whey separated protein powder soluble in water, the whey protein isolate solution that mass concentration is 7% is made, in 73MPa Under high-pressure homogeneous processing 2.5min, then freeze-drying form whey protein isolate freeze-dried powder.
After whey protein isolate freeze-dried powder is mixed with water by weight 100:5, it is sent into twin (double) screw extruder, and set The screw speed of twin (double) screw extruder is 100rpm, 45 DEG C of barrel temperature, die head length are 25mm.After to be extruded, it will squeeze Press-powder takes out and is freeze-dried, then crushes and cross 80 meshes, and high gel type whey separated protein powder can be obtained.
Embodiment 6
It takes whey separated protein powder soluble in water, the whey protein isolate solution that mass concentration is 8.5%, In is made High-pressure homogeneous processing 4min under 92MPa, then freeze-drying forms whey protein isolate freeze-dried powder.
After whey protein isolate freeze-dried powder is mixed with water by weight 100:3, it is sent into twin (double) screw extruder, and set The screw speed of twin (double) screw extruder is 130rpm, 42 DEG C of barrel temperature, die head length are 27mm.After to be extruded, it will squeeze Press-powder takes out and is freeze-dried, then crushes and cross 80 meshes, and high gel type whey separated protein powder can be obtained.
Embodiment 7
It takes whey separated protein powder soluble in water, the whey protein isolate solution that mass concentration is 8% is made, in 80MPa Under high-pressure homogeneous processing 3min, then freeze-drying form whey protein isolate freeze-dried powder.
After whey protein isolate freeze-dried powder is mixed with water by weight 100:7, it is sent into twin (double) screw extruder, and set The screw speed of twin (double) screw extruder is 90rpm, 50 DEG C of barrel temperature, die head length are 30mm.After to be extruded, it will squeeze Powder takes out and is freeze-dried, then crushes and cross 80 meshes, and high gel type whey separated protein powder can be obtained.
Embodiment 8
It takes whey separated protein powder soluble in water, the whey protein isolate solution that mass concentration is 9% is made, in 85MPa Under high-pressure homogeneous processing 2.9min, then freeze-drying form whey protein isolate freeze-dried powder.
After whey protein isolate freeze-dried powder is mixed with water by weight 100:6, it is sent into twin (double) screw extruder, and set The screw speed of twin (double) screw extruder is 120rpm, 35 DEG C of barrel temperature, die head length are 29mm.After to be extruded, it will squeeze Press-powder takes out and is freeze-dried, then crushes and cross 80 meshes, and high gel type whey separated protein powder can be obtained.
Using Texture instrument detection the various embodiments described above made from high gel type whey separated protein powder gel characteristic and with The common whey separated protein powder of unprocessed mistake compares.Use the cylindrical type probe (P/6) that diameter is 6mm with 1mm/s Constant speed penetrate gel, gel piece height is 10mm, diameter 24mm.Gel hardness is in the first second compression circulation of probe Maximum, force indicate that unit indicates with g.Each condition takes 3 sample measurements to be averaged.As a result as shown in the table.
As can be seen from the above table, the gel hardness of each embodiment whey separated protein powder and water-retaining property are much higher than common cream Clear separated protein powder illustrates that preparation method of the present invention improves the gel characteristic of whey separated protein powder.And embodiment 5 to 8 is made Three numerical value of the whey separated protein powder obtained are higher than embodiment 1 to 4, illustrate high-pressure homogeneous and extrusion combination further increasing Strong modified effect, improves the gel characteristic of whey separated protein powder.
The above is only a preferred embodiment of the present invention, is not intended to limit the scope of the invention, for this field For technical staff, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any Modification, equivalent replacement, improvement etc. should all be included within the scope of the present invention.

Claims (9)

1. a kind of preparation method of high gel type whey separated protein powder, which comprises the following steps:
Extrusion is handled after whey protein isolate freeze-dried powder is mixed with water, obtains extruding powder;
After the extruding powder freeze-drying process, high gel type whey separated protein powder is crushed to obtain.
2. the preparation method of high gel type whey separated protein powder as described in claim 1, which is characterized in that described by whey In the step of extrusion is handled after protein isolate freeze-dried powder is mixed with water, must squeeze powder, the whey protein isolate freeze-dried powder Weight ratio with water is 100:(3~7).
3. the preparation method of high gel type whey separated protein powder as claimed in claim 2, which is characterized in that the whey point It is 100:5 from the weight ratio of protein freeze-dried powder and water.
4. the preparation method of high gel type whey separated protein powder as described in claim 1, which is characterized in that described by whey In the step of extrusion is handled after protein isolate freeze-dried powder is mixed with water, must squeeze powder, the technological parameter of the extrusion Are as follows: 90~130rpm of screw speed, 35~50 DEG C of barrel temperature, 25~30mm of die head length.
5. the preparation method of high gel type whey separated protein powder as claimed in claim 4, which is characterized in that the extruding is swollen The technological parameter of change are as follows: screw speed 100rpm, 45 DEG C of barrel temperature, die head length 25mm.
6. the preparation method of high gel type whey separated protein powder as described in claim 1, which is characterized in that described by whey Before the step of extrusion is handled after protein isolate freeze-dried powder is mixed with water, must squeeze powder further include:
Whey separated protein powder is dissolved in water, forms whey protein isolate solution;
Under 73~92MPa, to the whey protein isolate solution homogenization;
By the whey protein isolate solution freeze-drying after homogenization, whey protein isolate freeze-dried powder is obtained.
7. the preparation method of high gel type whey separated protein powder as claimed in claim 6, which is characterized in that described by whey In the step of separated protein powder is dissolved in water, forms whey protein isolate solution, the mass concentration of the whey protein isolate solution It is 7%~9%.
8. the preparation method of high gel type whey separated protein powder as claimed in claim 6, which is characterized in that it is set forth in 73~ Under 92MPa, the step of to the whey protein isolate solution homogenization in, the time of the homogenization is 2.5~4min.
9. the preparation method of high gel type whey separated protein powder as described in claim 1, which is characterized in that it is described will be described After squeezing powder freeze-drying process, crush high gel type whey separated protein powder the step of in, the high gel type whey point Partial size from albumen powder is not more than 80 mesh.
CN201910820265.9A 2019-08-30 2019-08-30 A kind of preparation method of high gel type whey separated protein powder Pending CN110419621A (en)

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Publication number Priority date Publication date Assignee Title
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Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
AU1072388A (en) * 1987-01-26 1988-07-28 Director General of The Ministry of Agriculture and Fisheries, The Puffed food products and methods of making such products
US20110014328A1 (en) * 2009-06-26 2011-01-20 Cornell University Functionally superior whey proteins
CN104872373A (en) * 2015-05-12 2015-09-02 东北农业大学 Novel preparation process for improving gel stability of soy isolate protein

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