CN110412301A - The operating method of bracket for blood grouping - Google Patents
The operating method of bracket for blood grouping Download PDFInfo
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- CN110412301A CN110412301A CN201910720769.3A CN201910720769A CN110412301A CN 110412301 A CN110412301 A CN 110412301A CN 201910720769 A CN201910720769 A CN 201910720769A CN 110412301 A CN110412301 A CN 110412301A
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- blood
- bracket
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- cell
- sample
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- 210000004369 blood Anatomy 0.000 title claims abstract description 46
- 239000008280 blood Substances 0.000 title claims abstract description 46
- 238000011017 operating method Methods 0.000 title claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 30
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 21
- 238000012360 testing method Methods 0.000 claims abstract description 15
- 210000004027 cell Anatomy 0.000 claims abstract description 5
- 239000003153 chemical reaction reagent Substances 0.000 claims description 10
- 239000003146 anticoagulant agent Substances 0.000 claims description 9
- 229940127219 anticoagulant drug Drugs 0.000 claims description 9
- 239000000725 suspension Substances 0.000 claims description 8
- 239000006285 cell suspension Substances 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 5
- 238000002474 experimental method Methods 0.000 claims description 4
- 230000032258 transport Effects 0.000 claims description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 3
- 206010018910 Haemolysis Diseases 0.000 claims description 3
- 238000010241 blood sampling Methods 0.000 claims description 3
- 239000003292 glue Substances 0.000 claims description 3
- 230000008588 hemolysis Effects 0.000 claims description 3
- 239000003550 marker Substances 0.000 claims description 3
- 210000005259 peripheral blood Anatomy 0.000 claims description 3
- 239000011886 peripheral blood Substances 0.000 claims description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 210000002381 plasma Anatomy 0.000 claims description 2
- 210000002966 serum Anatomy 0.000 claims description 2
- 239000011780 sodium chloride Substances 0.000 claims description 2
- 235000013399 edible fruits Nutrition 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 239000000427 antigen Substances 0.000 abstract description 9
- 102000036639 antigens Human genes 0.000 abstract description 9
- 108091007433 antigens Proteins 0.000 abstract description 9
- 102000004169 proteins and genes Human genes 0.000 abstract description 7
- 108090000623 proteins and genes Proteins 0.000 abstract description 7
- 230000004523 agglutinating effect Effects 0.000 abstract description 6
- 101000874347 Streptococcus agalactiae IgA FC receptor Proteins 0.000 abstract description 4
- 102100027544 Blood group Rh(D) polypeptide Human genes 0.000 abstract description 3
- 101000580024 Homo sapiens Blood group Rh(D) polypeptide Proteins 0.000 abstract description 3
- 238000012797 qualification Methods 0.000 abstract description 3
- 241000950637 Henricia tumida Species 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses the operating methods of bracket for blood grouping, and according on red blood cell, whether there is or not Staphylococal Protein A or B antigens, in addition, according to erythrocyte blood type whether there is or not Staphylococal Protein A and B antigen, can be divided into A type, Type B, AB type and O-shaped on red blood cell.According to the agglutinating reaction of anti-A and anti-B antibody and corresponding red blood cell, abo blood group can be identified.According to the agglutinating reaction of Antibodies Against Rhesus D Antigen and corresponding red cell antigens, distinguishes the RhD positive and RhD is negative.Method of the invention is for slide method, the effective error for reducing bracket for blood grouping, can carry out authentication process to blood group without using centrifuge for test tube method, it follows that bracket for blood grouping method error of the invention is small, qualification process is extremely convenient.
Description
Technical field
The invention belongs to bracket for blood grouping technical field more particularly to the operating methods of bracket for blood grouping.
Background technique
Abo blood group identification is the detection for referring to ABH blood group antigens.Red blood cell is A type containing Staphylococal Protein A, and the antigen containing B is B
Type, the antigen containing A and B are AB type；Without A, B antigen, and the title of the antigen containing H is O-shaped.
Currently, traditional bracket for blood grouping mode is to be identified by the way of slide method or test tube method blood group, Yin Bo
The bracket for blood grouping mode error of piece method is big, in addition, test tube method needs to be operated using centrifuge, such mode of operation is time-consuming to take
Power, operation is extremely inconvenient, and in view of the above drawbacks, it is really necessary to the operating methods of necessary design bracket for blood grouping.
Summary of the invention
Technical problem to be solved by the present invention lies in: the operating method of bracket for blood grouping is provided, is mentioned to solve background technique
Out the problem of.
In order to solve the above technical problems, the technical scheme is that the operating method of bracket for blood grouping, including sample are adopted
Collection, sample transport, specimen storage, marked, reagent addition, red cell suspension addition, stirring, observation, judgement and experiment, are pressed
Following steps carry out:
A, collection of specimens: checked object venous blood is acquired with EDTA anticoagulant tube, is mixed well, it is ensured that is anticoagulant abundant.Promptly
Anticoagulant arterial blood can be used in situation, and infant and other patients for being not easy to vacuum blood sampling tube such as can without reverse type test
Acquire peripheral blood；
B, sample transports: the sample and the request slip same period being collected were sent to Blood Transfusion Dept. in 2 hours；
C, specimen storage: sample should be placed under 2-8C environment and be stored；
D, tri- regions A, B and D marked: are divided into micro-pipe with marker pen；
E, reagent adds: adding the anti-A of 1 drop, anti-B and anti-D blood grouping reagent respectively on the slide marked；
F, red cell suspension adds: respectively adding 1 10% erythrocyte suspension of drop again；
G, it stirs: red blood cell being ceased into liquid with clean stirring rod and reagent is sufficiently mixed, 20 microlitres of mixing are sucked in micro-pipe
Liquid；
H, it observes: micro-pipe is inserted on cystosepiment vertically, observe result and record；
I, determine；Completely integrate sinking or haemolysis occurs as positive findings, is still yin in suspension state after cell mixing 10min
Property result；
J, it tests: doubting glue method using test tube method or microtrabeculae and the result after judgement is carried out to repeat experiment.
Further, the sample in the step C specimen storage should be saved at least 7 days, in case result is checked.
Further, the red delicate suspension in the step F red cell suspension addition can be saline suspension, be also possible to hang
Float on the red blood cell in self blood plasma or serum.
Further, it is 5-10min that the micro-pipe in step H observation is inserted in the time on cystosepiment vertically.
Compared with prior art, the operating method of the bracket for blood grouping, according on red blood cell, whether there is or not Staphylococal Protein A or B antigens, separately
Outside, according to erythrocyte blood type whether there is or not Staphylococal Protein A and B antigen, can be divided into A type, Type B, AB type and O-shaped on red blood cell.According to anti-
The agglutinating reaction of A and anti-B antibody and corresponding red blood cell, can identify abo blood group.It is anti-with corresponding red blood cell according to Antibodies Against Rhesus D Antigen
Former agglutinating reaction, distinguishes the RhD positive and RhD is negative.Method of the invention effectively reduces blood group for slide method
The error of identification can carry out authentication process to blood group without using centrifuge for test tube method, it follows that this hair
Bright bracket for blood grouping method error is small, and qualification process is extremely convenient.
Detailed description of the invention
Fig. 1 is the flow chart of the operating method of bracket for blood grouping.
Specific embodiment
Hereinafter, a variety of specific details are elaborated, in order to provide to the saturating of the concept for constituting described embodiment basis
Thorough understanding.However, it will be apparent to those skilled in the art that described embodiment can be in these no specific details
In some or all situations get off practice.In other cases, well-known processing step is not specifically described.
Embodiment
The operating method for the bracket for blood grouping that the present embodiment is enumerated, is made as follows, is acquired with EDTA anticoagulant tube tested
Object venous blood, mixes well, it is ensured that anticoagulant abundant.Anticoagulant arterial blood, infant and other inconvenience can be used in emergency
Peripheral blood such as can be acquired without reverse type test in the patient of vacuum blood sampling tube, the sample being collected and the request slip same period are 2
It is sent to Blood Transfusion Dept. in hour, sample should be placed under 2-8C environment and be stored, and be divided into A, B and D tri- in micro-pipe with marker pen
A region adds the anti-A of 1 drop, anti-B and anti-D blood grouping reagent respectively on the slide marked, then respectively adds 1 10% patient of drop
Red blood cell is ceased liquid with clean stirring rod and reagent is sufficiently mixed by red cell suspension, and 20 microlitres of mixed liquors are sucked in micro-pipe, will
Micro-pipe is inserted on cystosepiment vertically, is observed result and is recorded, and completely integrates sinking or haemolysis occurs as positive findings, cell mixes
In suspension state it is still negative findings after 10min, doubts glue method using test tube method or microtrabeculae and the result after judgement is repeated
Experiment.
The operating method of the bracket for blood grouping, according on red blood cell, whether there is or not Staphylococal Protein A or B antigens, in addition, having according on red blood cell
Without Staphylococal Protein A and B antigen, erythrocyte blood type can be divided into A type, Type B, AB type and O-shaped.According to anti-A and anti-B antibody with it is corresponding
The agglutinating reaction of red blood cell can identify abo blood group.According to the agglutinating reaction of Antibodies Against Rhesus D Antigen and corresponding red cell antigens, distinguish
The RhD positive and RhD are negative.Method of the invention is for slide method, the effective error for reducing bracket for blood grouping, relative to
Authentication process can be carried out to blood group without using centrifuge for test tube method, it follows that bracket for blood grouping method of the invention
Error is small, and qualification process is extremely convenient, in addition, instrument used in the present invention and material are anti-A/B blood star styling agent
(monoclonal antibody) RhD (IgM) blood grouping reagent (monoclonal antibody), micro-pipe, rack for test tube, disposable plastic dropper, mark
Pen.
The present invention is not limited to above-mentioned specific embodiment, those skilled in the art from the above idea,
Without creative labor, the various transformation made are within the scope of the present invention.
Claims (4)
1. the operating method of bracket for blood grouping, it is characterised in that including collection of specimens, sample transport, specimen storage, marked, examination
Agent addition, stirring, observation, determines and tests red cell suspension addition, sequentially includes the following steps:
A, collection of specimens: checked object venous blood is acquired with EDTA anticoagulant tube, is mixed well, it is ensured that is anticoagulant abundant.Emergency
Anticoagulant arterial blood can be used, infant and other patients for being not easy to vacuum blood sampling tube can such as acquire without reverse type test
Peripheral blood；
B, sample transports: the sample and the request slip same period being collected were sent to Blood Transfusion Dept. in 2 hours；
C, specimen storage: sample should be placed under 2-8C environment and be stored；
D, tri- regions A, B and D marked: are divided into micro-pipe with marker pen；
E, reagent adds: adding the anti-A of 1 drop, anti-B and anti-D blood grouping reagent respectively on the slide marked；
F, red cell suspension adds: respectively adding 1 10% erythrocyte suspension of drop again；
G, it stirs: red blood cell being ceased into liquid with clean stirring rod and reagent is sufficiently mixed, 20 microlitres of mixed liquors are sucked in micro-pipe；
H, it observes: micro-pipe is inserted on cystosepiment vertically, observe result and record；
I, determine；Completely integrate sinking or occur haemolysis as positive findings, cell mix 10min after still in suspension state be feminine gender tie
Fruit；
J, it tests: doubting glue method using test tube method or microtrabeculae and the result after judgement is carried out to repeat experiment.
2. the operating method of bracket for blood grouping according to claim 1, wherein the sample in the step C specimen storage should be saved at least
7 days, in case result is checked.
3. the operating method of bracket for blood grouping according to claim 1, wherein red delicate outstanding in step F red cell suspension addition
Liquid can be saline suspension, be also possible to the red blood cell being suspended in self blood plasma or serum.
4. the operating method of bracket for blood grouping according to claim 1, wherein the micro-pipe in step H observation is inserted in cystosepiment vertically
The upper time is 5-10min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910720769.3A CN110412301A (en) | 2019-08-06 | 2019-08-06 | The operating method of bracket for blood grouping |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910720769.3A CN110412301A (en) | 2019-08-06 | 2019-08-06 | The operating method of bracket for blood grouping |
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| Publication Number | Publication Date |
|---|---|
| CN110412301A true CN110412301A (en) | 2019-11-05 |
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ID=68366064
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910720769.3A Pending CN110412301A (en) | 2019-08-06 | 2019-08-06 | The operating method of bracket for blood grouping |
Country Status (1)
| Country | Link |
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| CN (1) | CN110412301A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1904618A (en) * | 2006-07-31 | 2007-01-31 | 潘干华 | Method of implementing erythrocyte blood group antigen detection on haemocyte analysis instrument |
| CN1982894A (en) * | 1999-06-08 | 2007-06-20 | 奥索临床诊断有限公司 | Simultaneous determination of forward and reverse ABO blood group |
| US20070218515A1 (en) * | 2006-03-14 | 2007-09-20 | Nina Zhou | Method and kits for the determination of the antigens on the red blood cells and antibodies of serum |
| CN106290922A (en) * | 2016-09-20 | 2017-01-04 | 刘大基 | ABO is positive and negative and RhD antigens bloodtypes identifies combiner and authentication method |
-
2019
- 2019-08-06 CN CN201910720769.3A patent/CN110412301A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1982894A (en) * | 1999-06-08 | 2007-06-20 | 奥索临床诊断有限公司 | Simultaneous determination of forward and reverse ABO blood group |
| US20070218515A1 (en) * | 2006-03-14 | 2007-09-20 | Nina Zhou | Method and kits for the determination of the antigens on the red blood cells and antibodies of serum |
| CN1904618A (en) * | 2006-07-31 | 2007-01-31 | 潘干华 | Method of implementing erythrocyte blood group antigen detection on haemocyte analysis instrument |
| CN106290922A (en) * | 2016-09-20 | 2017-01-04 | 刘大基 | ABO is positive and negative and RhD antigens bloodtypes identifies combiner and authentication method |
Non-Patent Citations (1)
| Title |
|---|
| 张丽娜, 长春：吉林科学技术出版社 * |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191105 |
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