CN110403919A - A kind of preparation method of rouge radish thionin capsule of nano - Google Patents
A kind of preparation method of rouge radish thionin capsule of nano Download PDFInfo
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- CN110403919A CN110403919A CN201910808660.5A CN201910808660A CN110403919A CN 110403919 A CN110403919 A CN 110403919A CN 201910808660 A CN201910808660 A CN 201910808660A CN 110403919 A CN110403919 A CN 110403919A
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- capsule
- nano
- solution
- thionin
- rouge radish
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- 239000002775 capsule Substances 0.000 title claims abstract description 77
- 108010076830 Thionins Proteins 0.000 title claims abstract description 40
- 241000220259 Raphanus Species 0.000 title claims abstract description 37
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 title claims abstract description 37
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 title 1
- PVPBBTJXIKFICP-UHFFFAOYSA-N (7-aminophenothiazin-3-ylidene)azanium;chloride Chemical compound [Cl-].C1=CC(=[NH2+])C=C2SC3=CC(N)=CC=C3N=C21 PVPBBTJXIKFICP-UHFFFAOYSA-N 0.000 claims abstract description 39
- 238000004945 emulsification Methods 0.000 claims abstract description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 108010010803 Gelatin Proteins 0.000 claims abstract description 13
- 229920000159 gelatin Polymers 0.000 claims abstract description 13
- 239000008273 gelatin Substances 0.000 claims abstract description 13
- 235000019322 gelatine Nutrition 0.000 claims abstract description 13
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 13
- 229920001661 Chitosan Polymers 0.000 claims abstract description 11
- 239000000287 crude extract Substances 0.000 claims abstract description 9
- 238000006243 chemical reaction Methods 0.000 claims abstract description 8
- 239000004094 surface-active agent Substances 0.000 claims abstract description 8
- 238000003756 stirring Methods 0.000 claims abstract description 6
- 235000015112 vegetable and seed oil Nutrition 0.000 claims abstract description 6
- 239000008158 vegetable oil Substances 0.000 claims abstract description 6
- 238000007605 air drying Methods 0.000 claims abstract description 4
- 239000012153 distilled water Substances 0.000 claims abstract description 4
- 150000003839 salts Chemical class 0.000 claims abstract description 4
- 238000002525 ultrasonication Methods 0.000 claims abstract description 4
- QKGJFQMGPDVOQE-UHFFFAOYSA-N Sulforaphen Natural products CS(=O)C=CCCN=C=S QKGJFQMGPDVOQE-UHFFFAOYSA-N 0.000 claims description 83
- QKGJFQMGPDVOQE-HWKANZROSA-N raphanin Chemical compound CS(=O)\C=C\CCN=C=S QKGJFQMGPDVOQE-HWKANZROSA-N 0.000 claims description 83
- 239000000839 emulsion Substances 0.000 claims description 11
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 9
- 238000005119 centrifugation Methods 0.000 claims description 6
- 239000003921 oil Substances 0.000 claims description 6
- 235000019198 oils Nutrition 0.000 claims description 6
- 239000008159 sesame oil Substances 0.000 claims description 4
- 235000011803 sesame oil Nutrition 0.000 claims description 4
- 239000004359 castor oil Substances 0.000 claims description 3
- 235000019438 castor oil Nutrition 0.000 claims description 3
- 238000004090 dissolution Methods 0.000 claims description 3
- 230000001804 emulsifying effect Effects 0.000 claims description 3
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 claims description 3
- 229940051841 polyoxyethylene ether Drugs 0.000 claims description 3
- 229920000056 polyoxyethylene ether Polymers 0.000 claims description 3
- 239000006185 dispersion Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims 1
- 239000000843 powder Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 12
- 238000000034 method Methods 0.000 abstract description 7
- 230000002411 adverse Effects 0.000 abstract description 4
- 238000013270 controlled release Methods 0.000 abstract description 3
- 239000011858 nanopowder Substances 0.000 abstract description 3
- 238000001816 cooling Methods 0.000 abstract description 2
- 238000007711 solidification Methods 0.000 abstract 2
- 230000008023 solidification Effects 0.000 abstract 2
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 21
- 239000011162 core material Substances 0.000 description 15
- 230000014759 maintenance of location Effects 0.000 description 9
- 239000000463 material Substances 0.000 description 9
- 238000002835 absorbance Methods 0.000 description 7
- 239000003995 emulsifying agent Substances 0.000 description 7
- 239000003094 microcapsule Substances 0.000 description 6
- 230000036961 partial effect Effects 0.000 description 6
- 238000003860 storage Methods 0.000 description 6
- 239000000654 additive Substances 0.000 description 5
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- 238000010586 diagram Methods 0.000 description 5
- 238000005259 measurement Methods 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- SUVMJBTUFCVSAD-UHFFFAOYSA-N sulforaphane Chemical compound CS(=O)CCCCN=C=S SUVMJBTUFCVSAD-UHFFFAOYSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 230000001093 anti-cancer Effects 0.000 description 3
- 229960000935 dehydrated alcohol Drugs 0.000 description 3
- 239000003292 glue Substances 0.000 description 3
- SUVMJBTUFCVSAD-JTQLQIEISA-N 4-Methylsulfinylbutyl isothiocyanate Natural products C[S@](=O)CCCCN=C=S SUVMJBTUFCVSAD-JTQLQIEISA-N 0.000 description 2
- RUQCCAGSFPUGSZ-OBWQKADXSA-N Glucoraphanin Natural products C[S@](=O)CCCCC(=NS(=O)(=O)O)S[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O RUQCCAGSFPUGSZ-OBWQKADXSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- GMMLNKINDDUDCF-JRWRFYLSSA-N [(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] (1e)-5-[(r)-methylsulfinyl]-n-sulfooxypentanimidothioate Chemical compound C[S@@](=O)CCCC\C(=N/OS(O)(=O)=O)S[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O GMMLNKINDDUDCF-JRWRFYLSSA-N 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
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- 239000010408 film Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- -1 isosulfocyanate compound Chemical class 0.000 description 2
- 239000006210 lotion Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000010773 plant oil Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 229960005559 sulforaphane Drugs 0.000 description 2
- 235000015487 sulforaphane Nutrition 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000002344 surface layer Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000219198 Brassica Species 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001446187 Kermes Species 0.000 description 1
- 206010061902 Pancreatic neoplasm Diseases 0.000 description 1
- 206010060862 Prostate cancer Diseases 0.000 description 1
- 208000000236 Prostatic Neoplasms Diseases 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-N acetic acid Substances CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
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- 150000001875 compounds Chemical class 0.000 description 1
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- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 208000014829 head and neck neoplasm Diseases 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- CXORMDKZEUMQHX-UHFFFAOYSA-N kermesic acid Chemical compound O=C1C2=C(O)C(O)=CC(O)=C2C(=O)C2=C1C=C(O)C(C(O)=O)=C2C CXORMDKZEUMQHX-UHFFFAOYSA-N 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
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- 230000007774 longterm Effects 0.000 description 1
- 208000015486 malignant pancreatic neoplasm Diseases 0.000 description 1
- 208000026037 malignant tumor of neck Diseases 0.000 description 1
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- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
- A23L29/275—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of animal origin, e.g. chitin
- A23L29/281—Proteins, e.g. gelatin or collagen
- A23L29/284—Gelatin; Collagen
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
- A61K31/26—Cyanate or isocyanate esters; Thiocyanate or isothiocyanate esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/51—Nanocapsules; Nanoparticles
- A61K9/5107—Excipients; Inactive ingredients
- A61K9/513—Organic macromolecular compounds; Dendrimers
- A61K9/5161—Polysaccharides, e.g. alginate, chitosan, cellulose derivatives; Cyclodextrin
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/51—Nanocapsules; Nanoparticles
- A61K9/5107—Excipients; Inactive ingredients
- A61K9/513—Organic macromolecular compounds; Dendrimers
- A61K9/5169—Proteins, e.g. albumin, gelatin
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention discloses a kind of preparation method of rouge radish thionin capsule of nano, includes the following steps: 1) to dissolve: gelatin being dissolved in distilled water, and rouge radish thionin crude extract and vegetable oil is added, adds nonionic surface active agent；2) it emulsifies: solution is subjected to high speed emulsification；3) it is ultrasonically treated: solution being subjected to ultrasonication, while chitosan solution is added dropwise；4) it adjusts pH: adjusting pH value of solution, isothermal reaction；5) solidify: resulting reaction solution is placed in the ice bath water containing salt, formaldehyde, stirring solidification are added after cooling down；6) it is centrifuged: the solution after solidification is centrifuged；7) dry: to collect product forced air drying and obtain rouge radish thionin capsule of nano powder.The present invention improves the stability of rouge radish thionin, is not generated the effect for being influenced, while giving full play to its slow-release controlled-release on it by extraneous adverse environment condition by combining rouge radish thionin with capsule of nano embedding techniques.
Description
Technical field
The present invention relates to food byproducts manufacture fields, and in particular to a kind of preparation of rouge radish thionin capsule of nano
Method.
Background technique
Sulforaphen (sulforaphane, 1- isothiocyanic acid -4- methyl sulphonyl butane) is also known as sulforaphane, is western blue
Spend glucorphanin (Glucoraphanin, the Glucoraphanin) warp in equal brassica plants
The isosulfocyanate compound that myrosin hydrolysis generates.The study found that sulforaphen has extensive pharmacological function, such as press down
Bacterium sterilization, participates in immune and protects stomach lining from damage etc. anti-oxidant, inhibition platelet aggregation.In recent years, sulforaphen exists
Anti-tumor aspect shows stronger effect, can proliferation to kinds of tumor cells and growth play inhibiting effect, such as uterine neck
Cancer, prostate cancer, cancer of pancreas and breast cancer etc. are one of strongest active substance of plant of effect in known cancer-resisting substance.
However, the stability of sulforaphen is poor, light, oxygen, high temperature, acid or alkali environment can be made the stability of sulforaphen
At seriously affecting, this inactivates sulforaphen easily during preservation, moreover, concentrating at present to the research of sulforaphen
Extraction, anticancer mechanism and other physiological functions in relation to sulforaphen etc., there are no the micro- glue of nanometer is made about by sulforaphen
How sulforaphen is made capsule of nano by the report of capsule, and effectively long-term preservation sulforaphen avoids external environment such as
The adverse effect that light, oxygen, temperature, pH generate it, while the validity period of sulforaphen can also be extended, play slow releasing function from
And realize more preferably anticancer effect, it is that technical staff has project to be solved.Currently, people take conventional capsule and micron mostly
The capsule of grade is embedded, and the preparation of nanoscale sulforaphen microcapsules is there is not yet register.
Summary of the invention
In view of the above shortcomings of the prior art, it is an object of the invention to solve the radish extracted from rouge radish
Thionin permanently effective can not save, be easy by external environment for example light, temperature, moisture, pH have an adverse effect to it the problem of,
How solution extends the validity period for playing the bioactivity of sulforaphen simultaneously, it is made to play the role of slow-release controlled-release.This hair
Bright to provide a kind of preparation method of rouge radish thionin capsule of nano, using rouge radish thionin as core material, gelatin, chitosan are
Sulforaphen capsule of nano is made in wall material, is conducive to the stability for improving sulforaphen, reduces external environment to sulforaphen
Adverse effect, while improving the bioactivity of sulforaphen, its made to play slow-release controlled-release effect.
The present invention adopts the following technical scheme:
A kind of preparation method of sulforaphen capsule of nano, includes the following steps:
1) dissolve: gelatin is dissolved in 200mL 60 by the quality according to wall core than preparing gelatin, chitosan and thionin crude extract
In DEG C distilled water, and the vegetable oil of a certain proportion of sulforaphen crude extract and phase homogenous quantities is added, constitutes oil and mutually form water packet
Suitable nonionic surface active agent is added in oil type in the above solution；
2) it emulsifies: the solution in step 1) after completely dissolution being subjected to high speed emulsification, emulsification a period of time, makes emulsion point
It dissipates uniform；
3) it is ultrasonically treated: the solution after emulsification in step 2) being subjected to ultrasonication, while it is poly- that a certain amount of shell is added dropwise
Sugar juice；
4) pH is adjusted: to step 3) resulting solution adjusting pH value of solution to 6.0~6.4,13~18min of isothermal reaction；
5) solidify: reaction solution obtained in step 4) being placed in the ice bath water containing a small amount of salt, is added after cooling down
1~5mL of formaldehyde solidifies 50~80min under 250~450r/min stirring；
6) it is centrifuged: 8~12min of centrifugation is carried out under the conditions of 4000~7000r/min to the solution after solidifying in step 5)；
7) dry: after centrifugation, to collect capsule of nano, 10~15h of forced air drying obtains rouge radish thionin at 40 DEG C
Capsule of nano powder.
The present invention is prepared by embedding sulforaphen into capsule of nano, while using ultrasonic wave auxiliary complex coacervation
Sulforaphen capsule of nano improves the partial size of sulforaphen capsule of nano with this, not only plays protection to sulforaphen and makees
With making the capsule being prepared that there is good stability in high temperature, alkalinity, light environment, while also having good slow
Effect is released, to expand the application range of sulforaphen.
Further, the wall core is than the quality for gelatin and chitosan: the quality of thionin crude extract, and the wall core ratio is 1
~5:1, wherein the mass ratio of the gelatin and chitosan is 1:1.From attached drawing 1 as can be seen that wall core compares sulforaphen nanometer
The embedding rate of microcapsules has a certain impact, and with the increase of wall core ratio, embedding rate is in the variation tendency of reduction after first increasing, when
When wall core ratio is 3:1, rouge radish thionin capsule of nano embedding rate reaches maximum, when wall core ratio is 4:1,5:1, with wall core ratio
To compare when 3:1, embedding rate does not have significant changes.This is because core material can not be embedded completely when wall material dosage is very few,
The thin intensity that will affect capsule of nano of the capsule of nano wall of preparation, it is lower to eventually lead to embedding rate: when wall material dosage increase,
Since wall material itself has viscosity, wall material excessively will cause the increase of emulsion viscosity, reduce rouge radish thionin capsule of nano
Embedding rate is not utilized and wastes secondly, will cause a part of wall material using excessive wall material, leads to the increase of cost.To sum up,
Wall core ratio is a key factor of rouge radish thionin capsule of nano preparation, therefore wall core is than being preferably 3:1.
Further, the vegetable oil being added in the step 1) is sesame oil.Although other plant oil also can replace sesame
Oil, but the capsule of nano encystation effect and bad of other plant oil preparation, embedding rate and partial size are all not so good as sesame oil preparation
Capsule of nano.
Further, nonionic surface active agent is castor oil polyoxyethylene ether 40 in the step 1), and the amount of addition is
The 0.5%~1.0% of gross mass.The preferred nonionic surface active agent castor oil polyoxyethylene ether 40 of emulsifier, can from attached drawing 2
To find out, in the case where ceteris paribus, when emulsifier additive amount is 0.7%, the embedding of rouge radish thionin capsule of nano
Rate is maximum.When emulsifier additive amount is 0.4%, 0.5%, 0.6%, embedding rate is relatively low, this is because emulsifier adds
Amount is very little, and sulforaphen cannot emulsify completely, have a small amount of emulsion film that cannot coat sulforaphen surface layer completely, in strong machine
Under tool stirring, is agglomerated after oil phase drop collision, embedding rate is caused to reduce.And when emulsifier additive amount is continuously increased excessive, cream
It is too big to change fluid viscosity, stirring difficulty increases, and cannot make emulsion homogeneous, cause under rouge radish thionin capsule of nano embedding rate
Drop.Emulsifier can be formed with the interfacial film of certain mechanical strength on oil-water interface, influence emulsion stability and the micro- glue of nanometer
Capsule embedding rate.Therefore, the additive amount of nonionic surface active agent is the 0.6% of gross mass.
Further, the revolving speed of step 2) the high speed emulsification is 8000~12000r/min.It can be seen that from attached drawing 3
In the case where other conditions are constant, with the increase of revolving speed, embedding takes the lead in reducing after increasing, and wraps when emulsifying revolving speed and being 10000r/min
The rate of burying reaches highest, embedding rate 64.27%.From 8000r/min to 10000r/min, the increase of revolving speed fills emulsification more
Point, emulsification revolving speed is too low, prevents sulforaphen from dispersing to cause to assemble well, causes rouge radish thionin capsule of nano packet
It is lower to bury rate.When emulsifying revolving speed is 10000r/min, emulsification is more abundant, and solution uniformity more preferably, makes embedding rate reach maximum,
It is further added by emulsification revolving speed, emulsification revolving speed is excessive, leads to core material dispersion excessively, and lotion surface layer has part sulforaphen not to be embedded,
And established lotion wall can be excessive thinning because of emulsification revolving speed, so that rupture, eventually leads to embedding rate reduction.Therefore synthesis is examined
Consider, emulsification rotational speed optimization is selected as 10000r/min.
Further, emulsification times are 4~8min in the step 2).From attached drawing 4 as can be seen that in ceteris paribus
When, when the emulsification pretreatment time increases to 5min, the embedding rate of rouge radish thionin capsule of nano reaches maximum, and embedding rate is
63.30%.The reason is that water phase must be more abundant with oily phase emulsification pretreatment, the emulsion of formation with the increase of emulsification pretreatment time
It is more stable, when emulsification times are shorter, oil cannot be mutually completely dispersed water phase and form stable emulsion.When emulsification pretreatment
Between when being continued growing more than 5min, embedding rate is lower and lower, this is because the lengthening of emulsification times, leads to emulsion temperature liter
Height destroys the stability of emulsion.Therefore, emulsification times selection 5min is more appropriate.
Further, the solution in the step 3) after emulsification carries out ultrasonic wave under the conditions of 40~50 DEG C, 250W~280W
Processing.From attached drawing 5 as can be seen that in the case where other factors remain unchanged, when ultrasonic power is 180W to 270W, kermes
The embedding rate of sulforaphen capsule of nano increases with the increase of ultrasonic power, when ultrasonic power is 270W, embedding rate
Hereafter maximum declines.The reason is that ultrasonic power is continuously increased, the cavitation that ultrasonic wave generates adds the speed of molecular vibration
Fastly, and then the diffusion velocity of sulforaphen molecule is accelerated, improves its embedding rate；When ultrasonic power is excessive, cavitation
The rupture of rouge radish thionin capsule of nano structure is caused, so that its embedding rate is declined.Ultrasonic power is to rouge radish
The preparation of thionin capsule of nano has a certain impact, therefore ultrasonic power optimum choice is 270W.
Compared with prior art, the invention has the following beneficial effects:
1, the present invention greatly improves the stability of sulforaphen by the way that capsule of nano is made in sulforaphen, so that
The capsule of nano arrived has good photophobism, heat resistance, alkali resistance, can save the longer time in a natural environment,
Convenient for following process and sterilizing, to expand the application range of sulforaphen.
2, the present invention is adjusted the partial size of sulforaphen capsule of nano by ultrasonic wave auxiliary, is convenient for material into one
Surface modification is walked, embedding rate is improved, changes distribution, while making it have good targeting, convenient for sulforaphen anti-
Application in terms of cancer.In optimal conditions, it is 897 ± 25nm that the average grain diameter of microcapsules, which has reached average grain diameter, compared with other
Common micron order (1~1000mm) microcapsules, partial size are smaller；If high speed emulsification and ultrasonic wave auxiliary phase is not used to combine,
It is that cannot achieve nanoscale or only with their one such technologies, partial size is typically all micron order or the micro- glue of grade
Capsule, the present invention are greatly reduced the partial size of microcapsules by the combination of the two, nanoscale are reduced to from micron order, to improve
Its slow release and stability；The sulforaphen capsule of nano of this method preparation slow release of 3d in simulated intestinal fluid reaches
95% or more, it is significantly higher than nanoscale sulforaphen microcapsules.
3, the wall material that the present invention uses is to make capsule of nano biofacies with biological degradation polyalcohol gelatin and chitosan
Capacitive is good, and energy degradation in vivo, toxic side effect is small, while having slow releasing function, and slow-releasing granules can be made, and extends drug and treats
Effect, to realize more preferably anticancer effect.
Detailed description of the invention
Fig. 1 is the influence diagram that wall core of the present invention compares capsule of nano embedding rate.
Fig. 2 is influence diagram of the present invention emulsification revolving speed to capsule of nano embedding rate.
Fig. 3 is influence diagram of the emulsifier additive amount of the present invention to capsule of nano embedding rate.
Fig. 4 is influence diagram of the emulsification times of the present invention to capsule of nano embedding rate.
Fig. 5 is influence diagram of the ultrasonic power of the present invention to capsule of nano embedding rate.
Fig. 6 is sulforaphen content standard curve graph of the present invention.
Fig. 7 is 100 times of optical microscopes of capsule of nano prepared by the embodiment of the present invention 1.
Specific embodiment
Present invention will be further explained below with reference to the attached drawings and examples.
Embedding rate method for measuring are as follows:
1) sulforaphen standard curve making
The specific operation method is as follows: accurate measuring sulforaphen standard specimen 1mg, then measures 9mL dehydrated alcohol in beaker, matches
The sulforaphen standard solution for being 0.1mg/mL at concentration.Be respectively 0.10 according to concentration gradient compound concentration, 0.09,0.08,
0.07, the standard solution of 0.06,0.05,0.04mg/mL is successively to measure its absorbance at 201nm in wavelength, surveys 3 times, ask flat
Mean value.Then using measured absorbance as ordinate, with the content (mg/mL) of sulforaphen measurement for abscissa, standard is obtained
Curve is Y=0.6429X-0.0171, R2=0.9934, see attached drawing 6.Sulforaphen in sample is calculated according to standard curve to contain
Amount.
2) measurement of capsule of nano surface sulforaphen content
10mg rouge radish thionin capsule of nano is accurately weighed, 5min is extracted with 10mL dehydrated alcohol, in 4000r/min
It is centrifuged 15min under revolving speed, stands, records supernatant liquor volume, absorbance is measured after dilution at 201nm wavelength, according to recurrence
Thionin mass concentration and rouge radish thionin capsule of nano surface sulforaphen content is calculated in equation calculation solution.
3) in capsule of nano total sulforaphen content measurement
10mg rouge radish thionin capsule of nano is accurately weighed, 10mL dehydrated alcohol is added, it is ultrasonic under the conditions of 60 DEG C
20min is centrifuged 15min under 4000r/min revolving speed, stands, and records supernatant liquor volume, surveys at 201nm wavelength after dilution
Determine absorbance, calculate sulforaphen mass concentration in solution and calculates total sulforaphen content in rouge radish capsule of nano.
4) embedding rate calculates
Embodiment 1:
A kind of preparation method of sulforaphen capsule of nano, includes the following steps:
1) it dissolves: being 3:1 according to wall core ratio, prepare the quality of gelatin, chitosan and thionin crude extract, gelatin is dissolved in
In 60 DEG C of distilled water of 200mL, and 0.3g sulforaphen crude extract and the vegetable oil of 0.3g is added, constitutes oil and mutually form oil-in-water
Suitable nonionic surface active agent is added in type in the above solution；
2) it emulsifies: the solution in step 1) after completely dissolution being subjected to the high speed that revolving speed is 10000r/min and is emulsified, emulsification
5min makes emulsion be uniformly dispersed；
3) be ultrasonically treated: by the solution after emulsification in step 2) condition be power be 270W, temperature is 45 DEG C and surpasses
Sonicated, while 0.7% chitosan-acetic acid solution is added dropwise (0.5g chitosan is dissolved in 80mL1% acetum)；
4) it adjusts pH: pH value of solution is adjusted to 6.0~6.4,45 DEG C of isothermal reaction 15min to the resulting solution of step 3)；
5) solidify: reaction solution obtained in step 4) being placed in the ice bath water containing a small amount of salt, wait be cooled to room temperature
Formaldehyde 2mL is added, solidifies 60min under 300r/min stirring；
6) it is centrifuged: centrifugation 10min is carried out under the conditions of 5000r/min to the solution after solidifying in step 5)；
7) dry: after centrifugation, to collect capsule of nano, 10~15h of forced air drying obtains rouge radish thionin at 40 DEG C
Capsule of nano powder.
The capsule of nano embedding rate that the present embodiment is prepared is 65.24%, by 100 times of optical microphotograph sem observations,
It determining its grown form, sees attached drawing 7, it can be seen that capsule of nano has thin film to be wrapped in outer layer, and form is regular, complete,
Circle is presented, measuring average grain diameter using laser particle analyzer is 897 ± 25nm.
Embodiment 2~4 is the difference of related scale parameter to the main distinction of embodiment 1, is specifically shown in Table 1, other behaviour
Make all the same.
The 1 Examples 1 to 5 main distinction of table and test effect
The measurement of stability:
The sulforaphen capsule of nano that untreated sulforaphen is prepared with embodiment 1 is placed under the same terms and is stored up
After hiding for 24 hours, its retention rate is measured respectively, the results are shown in Table 2.
The calculating of retention rate:
The absorbance value for the sulforaphen that untreated sample ultraviolet specrophotometer can be surveyed, bringing formula into can
In the hope of the content of sulforaphen, the content of sulforaphen can be obtained multiplied by volume.Here it is original sulforaphens to contain
Amount.Processed sample can be measured by ultraviolet specrophotometer, and the absorbance value of sulforaphen, brings formula into after processing,
Finding out treated, sulforaphen concentration must arrive the sulforaphen content after processing after volume.Treated radish
Thionin content can find out divided by original sulforaphen content, after processing sulforaphen also retain how much, so as to find out
Retention rate.
So retention rate calculation formula is as follows:
Absorbance value measured by processed sample brings the concentration that standard curve acquires into
Sulforaphen capsule of nano (one) compared with the stability of sulforaphen under the different storage conditions of table 2
| Storage condition | Room temperature | 40℃ | 60℃ | 80℃ |
| Sulforaphen retention rate (after 10h) | 92 | 80 | 59 | 5 |
| Sulforaphen capsule of nano retention rate (after 10h) | 98 | 90 | 80 | 60 |
As can be seen from Table 2, sulforaphen capsule of nano under the high temperature conditions, has better compared to sulforaphen
Stability, this can greatly promote the application range of sulforaphen.
Sulforaphen capsule of nano (two) compared with the stability of sulforaphen under the different storage conditions of table 3
| Storage condition | PH=7 | PH=9 | PH=11 |
| Sulforaphen retention rate (after 10h) | 90 | 52 | 10 |
| Sulforaphen capsule of nano retention rate (after 10h) | 95 | 82 | 74 |
As can be seen from Table 3, sulforaphen capsule of nano under alkaline condition, has better compared to sulforaphen
Stability, this can greatly promote the application range of sulforaphen.
Sulforaphen capsule of nano (three) compared with the stability of sulforaphen under the different storage conditions of table 4
| Storage condition | Normal light shines | It is protected from light | Ultraviolet lighting |
| Sulforaphen retention rate (5 days) | 89 | 93 | 83 |
| Sulforaphen capsule of nano (5 days) | 93 | 96 | 86 |
As can be seen from Table 4, sulforaphen capsule of nano has good stability under illumination condition, easily facilitates
It saves.
In conclusion sulforaphen capsule of nano prepared by the present invention has good photophobism, heat resistance, alkaline-resisting
Property, the longer time can be saved in a natural environment.
Finally, it should be noted that technical side the above examples are only used to illustrate the technical scheme of the present invention and are not limiting
Case, those skilled in the art should understand that, modification or equivalent replacement of the technical solution of the present invention are made for those, and
The objective and range for not departing from the technical program, are intended to be within the scope of the claims of the invention.
Claims (7)
1. a kind of preparation method of rouge radish thionin capsule of nano, which comprises the steps of:
1) dissolve: gelatin is dissolved in 200 by the quality according to wall core than preparing gelatin, chitosan and rouge radish thionin crude extract
In 60 DEG C of distilled water of mL, and the vegetable oil of a certain proportion of sulforaphen crude extract and phase homogenous quantities is added, constitutes oil and mutually formed
Suitable nonionic surface active agent is added in oil-in-water type in the above solution；
2) it emulsifies: the solution in step 1) after completely dissolution being subjected to high speed emulsification, emulsification a period of time keeps emulsion dispersion equal
It is even；
3) it is ultrasonically treated: the solution after emulsifying in step 2 being subjected to ultrasonication, while it is molten that a certain amount of chitosan is added dropwise
Liquid；
4) it adjusts pH: pH value of solution is adjusted to 6.0 ~ 6.4,45 DEG C of 13 ~ 18min of isothermal reaction to the resulting solution of step 3)；
5) solidify: reaction solution obtained in step 4) is placed in the ice bath water containing a small amount of salt, be added wait be cooled to room temperature
1 ~ 5mL of formaldehyde solidifies 50 ~ 80min under 250 ~ 450r/min stirring；
6) it is centrifuged: 8 ~ 12min of centrifugation is carried out under the conditions of 4000 ~ 7000r/min to the solution after solidifying in step 5)；
7) dry: after centrifugation, to collect capsule of nano, it is micro- to obtain rouge radish thionin nanometer by 10 ~ 15h of forced air drying at 40 DEG C
Capsule powders.
2. the preparation method of rouge radish thionin capsule of nano according to claim 1, which is characterized in that the wall core
Than the quality for gelatin and chitosan: the quality of thionin crude extract, the wall core ratio are 1 ~ 5:1, wherein the gelatin and shell are poly-
The mass ratio of sugar is 1:1.
3. the preparation method of rouge radish thionin capsule of nano according to claim 1, which is characterized in that the step
1) vegetable oil being added in is sesame oil.
4. the preparation method of rouge radish thionin capsule of nano according to claim 1, which is characterized in that the step
1) nonionic surface active agent is castor oil polyoxyethylene ether 40 in, and the amount of addition is the 0.5% ~ 1.0% of gross mass.
5. the preparation method of rouge radish thionin capsule of nano according to claim 1, which is characterized in that the step
2) revolving speed of high speed emulsification is 8000 ~ 12000r/min.
6. the preparation method of rouge radish thionin capsule of nano according to claim 1, which is characterized in that the step
2) emulsification times are 4 ~ 8min in.
7. the preparation method of rouge radish thionin capsule of nano according to claim 1, which is characterized in that the step
3) solution in after emulsification carries out ultrasonication under the conditions of 40 ~ 50 DEG C, 250W ~ 280W.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111938155A (en) * | 2020-08-03 | 2020-11-17 | 中国农业科学院农产品加工研究所 | Embedding material of sulforaphane and preparation method thereof |
| CN114983963A (en) * | 2022-05-17 | 2022-09-02 | 上海玖思实业有限公司 | Preparation method of sulforaphane soft capsules |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103006572A (en) * | 2012-12-20 | 2013-04-03 | 浙江科技学院 | Preparation method of pH sensitiveness raphanin chitosan microsphere |
| CN103127031A (en) * | 2013-03-21 | 2013-06-05 | 黑龙江大学 | Preparation method for oily and oil soluble drug nanocapsules |
| US20140170218A1 (en) * | 2011-07-01 | 2014-06-19 | Theo Efstathiou | Compositions containing broccoli seeds for treatment or preventing prostate cancer |
| CN107569470A (en) * | 2017-09-30 | 2018-01-12 | 长江师范学院 | A kind of preparation method of sulforaphen microcapsules |
| CN109043533A (en) * | 2018-07-16 | 2018-12-21 | 浙江大学 | A method of sulforaphen microcapsules are prepared using emulsification spray drying process |
| CN109394729A (en) * | 2018-12-11 | 2019-03-01 | 曲阜师范大学 | A kind of medicine-carried system and preparation method thereof loading sulforaphen |
-
2019
- 2019-08-29 CN CN201910808660.5A patent/CN110403919B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140170218A1 (en) * | 2011-07-01 | 2014-06-19 | Theo Efstathiou | Compositions containing broccoli seeds for treatment or preventing prostate cancer |
| CN103006572A (en) * | 2012-12-20 | 2013-04-03 | 浙江科技学院 | Preparation method of pH sensitiveness raphanin chitosan microsphere |
| CN103127031A (en) * | 2013-03-21 | 2013-06-05 | 黑龙江大学 | Preparation method for oily and oil soluble drug nanocapsules |
| CN107569470A (en) * | 2017-09-30 | 2018-01-12 | 长江师范学院 | A kind of preparation method of sulforaphen microcapsules |
| CN109043533A (en) * | 2018-07-16 | 2018-12-21 | 浙江大学 | A method of sulforaphen microcapsules are prepared using emulsification spray drying process |
| CN109394729A (en) * | 2018-12-11 | 2019-03-01 | 曲阜师范大学 | A kind of medicine-carried system and preparation method thereof loading sulforaphen |
Non-Patent Citations (2)
| Title |
|---|
| 周伟华等: "《药物制剂技术及其发展探究》", 31 August 2017, 科学技术文献出版社 * |
| 阳晖等: "胭脂萝卜硫素微胶囊制备工艺优化", 《食品工业科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111938155A (en) * | 2020-08-03 | 2020-11-17 | 中国农业科学院农产品加工研究所 | Embedding material of sulforaphane and preparation method thereof |
| CN114983963A (en) * | 2022-05-17 | 2022-09-02 | 上海玖思实业有限公司 | Preparation method of sulforaphane soft capsules |
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