CN110384824A - A kind of three-stage functional form degradable artificial ligament regeneration support and preparation method thereof - Google Patents
A kind of three-stage functional form degradable artificial ligament regeneration support and preparation method thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/10—Ceramics or glasses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/12—Phosphorus-containing materials, e.g. apatite
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
- A61L2300/414—Growth factors
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/10—Materials or treatment for tissue regeneration for reconstruction of tendons or ligaments
Abstract
The invention discloses a kind of three-stage functional form degradable artificial ligament regeneration support and preparation method thereof, which includes: to prepare composite spinning solution or mixing wire drawing molten liquid；Prepare casting liquid；Prepare nano-fibre yams or long monofilament；Woven tubular textile fabric；Tubiform Textile Product is covered into the thermal finalization in the mold of assembling；Preparation has the first segment complex of cylindrical mold；Preparation has the first segment and second segment complex of cylindrical mold；Again casting liquid is continued to be cast in cylindrical mold, obtains three-stage bracket complex；Finally by three-stage bracket complex carry out electrostatic spinning to get.Using three-stage functional form degradable artificial ligament regeneration support prepared by the present invention, can Each performs its own functions in structure, there is good biomechanical property and biocompatibility, can be used for the regenerative therapy of such as rotator cuff, tendon, ligament injury disease；And preparation method is simple and efficient, and it is cheap, it has a good application prospect.
Description
Technical field
The present invention relates to a kind of three-stage functional form degradable artificial ligament and preparation method thereof technical fields, especially relate to
And a kind of rapidly promote and prevents dynamics enhancement type ligament regeneration support of postoperative loosening and preparation method thereof at tendon-bone.
Background technique
Effectively repairing again after the damage of anterior cruciate ligament (ACL) is current clinically a great problem, this is mainly completely disconnected
The ACL self-healing capability split is weak, and postoperative loosening rate and rate of breaking again are too high.Aspect that there are two the main reason for ACL reconstruction failure,
First is that the intrinsic property of the graft of reconstruction and natural ligament tissue cannot effectively skin match, such as Heterogeneous tendon, self tendon
Though and artificial ligament all be damage ligament optional substitute, there are the rejection of Heterogeneous tendon and self tendons
Carry out source problem；For another example artificial ligament LARS, be by bio-inert material polyethylene terephthalate (PET) processing and
At though starting in 1992 for clinical, material used in such ligament is without bioactivity and tissue induction
The ligament substitute of performance is only intended to mechanics load-bearing, and only in part, country enters clinic.The second of ligament reconstructive failure
A main cause is postoperative loosening rate and rate of breaking again is too high, and by the accumulation of many years, present rate of breaking again can pass through bone road position
It sets, i.e. surgical technic improvement is to reduce.In summary, we are summarized as, and ligament regenerates faced main problem: existing can not
The regeneration of induction ligament can not achieve physiological tendon-knitting, be easy the defects of failure.So clinical urgent need functional form
It can induce the regenerated artificial ligament of ligament and be implanted into bracket.
Fibroin albumen (SF) is the natural source protein of a kind of high-purity containing RGD sequence, biological safe type, is extensive
Promote tissue repair active material applied to one of tissue engineering bracket.SF suture has clinically applied more than 30 years, energy
Enough inhibit macrophage activity, apparent immune response and inflammatory reaction will not be caused as implantation material, immunogenicity is low.It is quiet
Although the nano fiber scaffold of Electrospinning preparation has certain mechanical property, but due to having too small hole, host
Cell can not normally be grown into nanofiber internal migration, and the quick weight for being extremely difficult to cambium is sticked only by physics
It builds, this just needs to combine by knitting skill and brittle failure-casting-mold molding-Freeze Drying Technique, and sub-module functionalization assigns
Tough belt supporting frame required function, makes it have multilayered structure, and Each performs its own functions, reaches clinical transplantation requirement.
Electrostatic spinning technique is widely used in preparing tissue engineering bracket, and knitting skill is also commonly used to prepare people
The technology of work ligament.But it is so far, dry using electrostatic spinning technique, knitting skill and brittle failure-casting-mold molding-freezing
The report for the method preparation ligament repair scaffold that dry technology combines but not yet occurs.Therefore one kind is prepared by this three kinds of technologies
The mature functional form three-dimensional ligament repair scaffold for integrating mechanical property and biocompatibility will have huge economic effect
Benefit.
Summary of the invention
The present invention is to solve the above problem in the prior art, proposes that one kind rapidly promotes tendon-bone and prevents postoperative loosening
Dynamics enhancement type ligament tissue bracket for regenerating and preparation method thereof.
To achieve the above object, the invention adopts the following technical scheme:
The first aspect of the invention is to provide a kind of preparation of three-stage functional form degradable artificial ligament regeneration support
Method, comprising:
Step 1: being in mass ratio that 1:1~4:1 dissolution or hot melt are mixed with fibroin albumen by high-molecular organic material, must answer
Spinning solution or mixing wire drawing molten liquid are closed, it is spare；
Step 2: by fibroin albumen, being loaded with the mesoporous nano-grain for promoting tendon-bone healing growth factor, rush tendon-knitting growth
Three kinds of components of the factor are 75:20:5~95:4:1 hot melt mixing in mass ratio, prepare certain density uniformly mixed casting liquid；
Step 3: the composite spinning solution of step 1) preparation or melt drawing solution are subjected to spinning or melt drawing respectively
Obtain different-diameter and length nano-fibre yams or long monofilament；
Step 4: the nano-fibre yams of step 3) preparation or long monofilament are woven into tubiform Textile Product；
Step 5: tubiform Textile Product made from step 4) being covered in the mold of assembling, it is dry that vacuum is integrally put into together with mold
Dry case is dry to carry out thermal finalization, the mechanics enhancement layer as artificial ligament；
Step 6: the casting liquid injection step 5 that step 2) is prepared the cylinder with the tubiform Textile Product after thermal finalization in)
In shape mold, together with the processing of mold vacuum freeze drying, the first segment complex with mold is obtained, it is spare；
Step 7: certain density silk fibroin protein solution is continued to be cast to first segment complex is had in step 6) again
In cylindrical mold, together with the processing of mold vacuum freeze drying, the first segment and second segment complex with mold are obtained, it is standby
With；
Step 8: the casting liquid that step 2) is prepared is continued to be cast in step 7) with first segment and second segment complex
Cylindrical mold in, together with the processing of mold vacuum freeze drying, obtain the three-stage bracket complex with mold, take off mould
It is spare after tool；
Step 9: three-stage bracket complex prepared by step 8) being fixed on electrostatic spinning apparatus, electrostatic spinning is passed through
Technology spins on composite spinning solution described in the step 1) that thickness is about 0.01mm~5mm or mixes the nanofiber of wire drawing liquid,
Obtain three-stage functional form degradable artificial ligament regeneration support.
Further, high-molecular organic material described in step 1) is selected from poly (lactic acid-glycolic acid) (PLGA), poly- two
Oxane ketone (PDO), polylactic acid (PLLA, PDLA, PDLLA), polyglycolic acid (PGA), polycaprolactone (PCL), poly- (lactic acid-is in oneself
Ester) (PLCL), polyhydroxyalkanoate (PHAs), polyester type biodegradable polyurethane, polyether-type Biodegradable polyurethane,
One or more of poly- (ester-ether) type Biodegradable polyurethane.
It is further preferred that the mass/volume concentration (w/v) of composite spinning solution described in step 1) is 5%~20%,
Its solvent is water, ethyl alcohol, dimethyl sulfoxide, Isosorbide-5-Nitrae-dioxane, hexafluoroisopropanol, n,N-Dimethylformamide, N, N- diformazan
One or more of yl acetamide, tetrahydrofuran, methylene chloride, acetone, chloroform, trifluoroacetic acid, trifluoroethanol.
Further, mesoporous nano-grain described in step 2) is the meso-porous nano that surface has hydroxyl, amino or carboxyl
Silicon, meso-porous nano hydroxyl phosphorite, meso-porous nano bata-tricalcium phosphate, meso-porous nano attapulgite, is situated between meso-porous nano bio-vitric
One or more of hole nano hydrotalcite, meso-porous nano illite, meso-porous nano rectorite or meso-porous nano montmorillonite；It is described
Growth factor is one of BMP-2, Deferoxamine, KGN, VEGF, dexamethasone, eugenol, chondroitin sulfate, interleukin or several
Kind.
It is further preferred that it is described be loaded with promote tendon-bone healing growth factor mesoporous nano-grain be the growth factor with
Pass through one of amidation process, ionic bonding reaction and physical absorption reaction or various ways between the mesoporous nano-grain
It is combined into.
Further, the mass/volume concentration (w/v) of casting liquid described in step 2) is 3.8%~52.4%.
Further, nano-fibre yams described in step 3) are using the long monofilament as the composite yarn of core, diameter
It is 10 μm~1000 μm.
Further, tubiform Textile Product internal diameter described in step 4) is 1mm~10mm, and length is 5cm~20cm.
Further, heat setting temperature described in step 5) is 35 DEG C~90 DEG C.
Further, mass/volume (w/v) concentration of silk fibroin protein solution described in step 7) is 4.8%~34.6%.
The second aspect of the invention is to provide a kind of three-stage functional form degradable artificial of method preparation described above
Ligament regeneration support.
The present invention by adopting the above technical scheme, compared with prior art, has the following technical effect that
(1) spinning material natural source fibroin albumen used in the sticking of cell, be proliferated and migration has good facilitation,
It is more advantageous to host cell and quickly covers with tough belt supporting frame, thus the synthesis height realizing rapid regeneration, while being blended with fibroin albumen
Molecular material can play the role of supporting newborn ligament tissue after fibroin albumen degradation；
(2) fibroin albumen/synthesis high molecular material composite nano fibre yarn line or monofilament are the intrinsic glue of natural human body
Former albumen and elastin laminin, the overall mechanics supporting layer as tough belt supporting frame；
(3) tough belt supporting frame both ends are nano particle and the finely dispersed promotion tendon-knitting gradient release life of the factor
The composite nanometer particle of the long factor/fibroin albumen three-dimensional structure, guidance promote osteoblast fast-growth, to reach quick
Rebuild tendon-bone parts；
(4) the compound three-stage functional form ligament regeneration support has good histocompatbility and biomechanics
Can, a kind of simple and effective technology of preparing thinking is provided to develop ligament tissue engineering rack.
Detailed description of the invention
Fig. 1 is the flow diagram of the preparation method of three-stage functional form degradable artificial ligament of the present invention；
Fig. 2 is the mesoporous bioglass nanometer that a- mesoporous bioglass nano particle transmission electron microscope and b- load have BMP-2
The transmission electron microscope picture of particle.
Specific embodiment
The present invention by electrostatic spinning technique, knitting skill and brittle failure-casting-mold molding-Freeze Drying Technique these three
Technology combines, and propose a kind of three-stage functional form degradable artificial ligament prepares forwarding method, using the three of this method preparation
Segmentation functional form degradable artificial ligament integrates mechanical property and biocompatibility, has huge economic results in society.
Refering to Figure 1, the present invention provides a kind of preparation of three-stage functional form degradable artificial ligament regeneration support
Method specifically comprises the following steps:
Step 1: being in mass ratio that 1:1~4:1 dissolution or hot melt are mixed with fibroin albumen by high-molecular organic material, must answer
Spinning solution or mixing wire drawing molten liquid are closed, it is spare；
Step 2: by fibroin albumen, being loaded with the mesoporous nano-grain for promoting tendon-bone healing growth factor, rush tendon-knitting growth
Three kinds of components of the factor are 75:20:5~95:4:1 hot melt mixing in mass ratio, prepare certain density uniformly mixed casting liquid；
Step 3: the composite spinning solution of step 1) preparation or melt drawing molten liquid being subjected to spinning respectively or melting is drawn
Silk obtain different-diameter and length nano-fibre yams or long monofilament；
Step 4: the nano-fibre yams of step 3) preparation or long monofilament are woven into tubiform Textile Product；
Step 5: tubiform Textile Product made from step 4) being covered in the mold of assembling, it is dry that vacuum is integrally put into together with mold
Dry case is dry to carry out thermal finalization, the mechanics enhancement layer as artificial ligament；
Step 6: the casting liquid injection step 5 that step 2) is prepared the cylinder with the tubiform Textile Product after thermal finalization in)
In shape mold, together with the processing of mold vacuum freeze drying, the first segment complex with mold is obtained, it is spare；
Step 7: certain density silk fibroin protein solution is continued to be cast to first segment complex is had in step 6) again
In cylindrical mold, together with the processing of mold vacuum freeze drying, the first segment and second segment complex with mold are obtained, it is standby
With；
Step 8: the casting liquid that step 2) is prepared is continued to be cast in step 7) with first segment and second segment complex
Cylindrical mold in, together with the processing of mold vacuum freeze drying, obtain the three-stage bracket complex with mold, take off mould
It is spare after tool；
Step 9: three-stage bracket complex prepared by step 8) being fixed on electrostatic spinning apparatus, electrostatic spinning is passed through
Technology spins on composite spinning solution described in the step 1) that thickness is about 0.01mm~5mm or mixes the nanofiber of wire drawing liquid,
Obtain three-stage functional form degradable artificial ligament regeneration support.
As an optimal technical scheme, in step 1), the quality of the high-molecular organic material and the fibroin albumen
Than being more preferably 1.5:1~3:1 for 1:1~4:1, preferably 1.2:1~3.5:1, more preferably 1.8:1~2.5:1.It is described
Fibroin albumen is extracted from tussah silkworm chrysalis；The high-molecular organic material is to dislike selected from poly (lactic acid-glycolic acid) (PLGA), poly- two
Alkanone (PDO), polylactic acid (PLLA, PDLA, PDLLA), polyglycolic acid (PGA), polycaprolactone (PCL), poly- (lactic acid-caprol acton)
(PLCL), polyhydroxyalkanoate (PHAs), polyester type biodegradable polyurethane, polyether-type Biodegradable polyurethane, poly-
One or more of (ester-ether) type Biodegradable polyurethane.
As an optimal technical scheme, the mass/volume concentration (w/v) of composite spinning solution described in step 1) is
5%~20%, preferably 8%~18%, more preferably 10%~16%, solvent is water, ethyl alcohol, dimethyl sulfoxide, Isosorbide-5-Nitrae-
Dioxane, hexafluoroisopropanol, n,N-Dimethylformamide, n,N-dimethylacetamide, tetrahydrofuran, methylene chloride, acetone,
One or more of chloroform, trifluoroacetic acid, trifluoroethanol.
As an optimal technical scheme, in step 2), the fibroin albumen is loaded with Jie for promoting tendon-bone healing growth factor
Hole nano particle promotees tendon-three kinds of components of knitting growth factor mass ratio as 75:20:5~95:4:1, preferably 78:17:5
~92:7:1 is more preferably 80:16:4~90:9:1, more preferably 85:12:3~88:10:2；And the quality of the casting liquid/
Volumetric concentration (w/v) is 3.8%~52.4%, preferably 8.6%~46.2%, is more preferably 18.2%~40.5%, more excellent
It is selected as 26.3%~32.8%.
The mesoporous nano-grain is meso-porous nano silicon, the meso-porous nano biology glass that surface has hydroxyl, amino or carboxyl
It is glass, meso-porous nano hydroxyl phosphorite, meso-porous nano bata-tricalcium phosphate, meso-porous nano attapulgite, meso-porous nano hydrotalcite, mesoporous
One or more of nanometer illite, meso-porous nano rectorite or meso-porous nano montmorillonite；The growth factor be BMP-2,
One or more of Deferoxamine, KGN, VEGF, dexamethasone, eugenol, chondroitin sulfate, interleukin.It is described to be loaded with rush tendon
The mesoporous nano-grain of knitting growth factor between the growth factor and the mesoporous nano-grain by amidation process,
One of ionic bonding reaction and physical absorption reaction or various ways are combined into.As shown in Figure 2, wherein a is unsupported
The transmission electron microscope picture of the mesoporous bioglass nano particle of biotic factor BMP-2, surface are porous laser pipe；B, which is negative, to be loaded with
The transmission electron microscope picture of the mesoporous bioglass nano particle of BMP-2, surface is non-porous, because small by biotic factor BMP-2 in hole
Molecule fills up.
As an optimal technical scheme, nano-fibre yams described in step 3) are composite yarn or non-composite yarn.
Preferably, the nano-fibre yams are a diameter of 10 μm~1000 μm using the long monofilament as the composite yarn of core, preferably
It is 80 μm~860 μm, is more preferably 180 μm~680 μm, more preferably 320 μm~460 μm.
As an optimal technical scheme, nano-fibre yams described in step 4) or long monofilament pass through drawing, 2~32 strands
Twisting post-processing can just be used to prepare tubiform Textile Product.And the tubiform Textile Product internal diameter be 1mm~10mm, length be 5cm~
20cm；Preferably, internal diameter is 2mm~8mm, and length is 6cm~18cm；More preferably, internal diameter is 3mm~6mm, and length is
8cm~15cm；It is further preferable that internal diameter is 4mm~5mm, length is 10cm~12cm.
As an optimal technical scheme, heat setting temperature described in step 5) be 35 DEG C~90 DEG C, preferably 42 DEG C~
85 DEG C, be more preferably 50 DEG C~80 DEG C, more preferably 65 DEG C~72 DEG C.
As an optimal technical scheme, mass/volume (w/v) concentration of silk fibroin protein solution described in step 7) is
4.8%~34.6%, preferably 8.2%~30.2%, it is more preferably 15.6%~24.8%, more preferably 18.3%~
20.4%.
The present invention also provides a kind of degradable induction ligament of three-stage functional form based on above method preparation is regenerated
Bracket, by electrostatic spinning technique, knitting skill, these three technologies are mutually tied with brittle failure-casting-mold molding-Freeze Drying Technique
Intersection mechanical property and biocompatibility are in being integrally prepared.
The degradable regenerated bracket of induction ligament of the three-stage functional form, enough Each performs its own functions in structure, artificial ligament two
End can rapidly promote after the transfer tendon-bone, middle section can rapid induction ligament tissue rebuild, weave reinforcing section early period
Play mechanical support, later period fast degradation generates big connected holes, be easy to host cell migration grow into.The three-stage functional form
The degradable induction regenerated bracket of ligament can be used for the regenerative therapy of such as rotator cuff, tendon, ligament injury disease, and its preparation side
Method is simple and efficient, cheap；The three-stage functional form biodegradable stent has good biomechanical property and bio-compatible
Property, and bracket biomethanics, three-dimensional structure and degradation cycle can be regulated and controled according to the feature of transplantation site, in transfer operation
To human body without hypersensitivity, while there is affinity with the intracorporal blood of people and perienchyma, has in surgery repairing operation
Good application prospect.
The present invention is described in more detail below by specific embodiment, for a better understanding of the present invention,
But following embodiments are not intended to limit the scope of the invention.
Embodiment 1
Preparation process shown in refering to fig. 1, the present embodiment provides a kind of regeneration of three-stage functional form degradable artificial ligament
The preparation method of bracket, specifically comprises the following steps:
Step 1: poly- (glycolic) (PGA)/tussah silk fibroin (SF) that mass ratio is 2:1 is weighed on assay balance,
It is dissolved in hexafluoroisopropanol under the conditions of 25 DEG C, stirring and dissolving is uniform molten to the clarification that mass volume ratio (g/mL) is 15% is obtained
Liquid；
Step 2: by fibroin albumen, being loaded with the mesoporous nano-grain for promoting tendon-bone healing growth factor, rush tendon-knitting growth
Three kinds of components of the factor are 75:20:5 dissolution according to mass ratio, prepare the casting liquid that mass-volume concentration is 20%；
Step 3: mixed solution spinning solution being then subjected to loudspeaker-conjugation electrostatic spinning, obtaining diameter is 300 μm
PGA/SF composite nano fibre yarn line；
Step 4: 8 bursts of doublings, twisting being used to prepare internal diameter in conjunction with common knitting skill as the tubular structure of 2mm, long 8cm
PGA/SF composite nano fibre yarn line mechanics enhancement layer；
Step 5: the mechanics enhancement layer of tubular structure and the ePTFE mold of length 8cm being fitted together again, are placed in 50 DEG C
Cooling, the mechanics enhancement layer as artificial ligament are taken out in thermal finalization in drying box；
Step 6: after tubiform Textile Product is cooling, group is divided into fibroin albumen, is loaded with BMP-2 mesoporous bioglass nanometer
The mass-volume concentration that particle, BMP-2 mass ratio are 75:20:5 be 20% casting liquid injection tubular braid and mold it is compound
In body, it is freeze-dried 24 hours, obtains first segment complex；
Step 7: again injecting the silk fibroin water solution that mass-volume concentration is 12% in first segment complex, freezing is dry
Dry 24 hours, obtain second segment complex；
Step 8: group being finally divided into fibroin albumen, the mesoporous bioglass nano particle for being loaded with BMP-2, BMP-2 mass
It is to be freeze-dried 24 hours, must weave in 20% casting liquid injection second segment complex than the mass-volume concentration for 75:20:5
The three-stage solid cylindrical bracket of reinforcement；
Step 9: the PGA/SF that one layer of mass ratio is 2:1 is finally sprayed by electrostatic spinning technique in columnar bracket outer surface
The degradable ligament regeneration support of three-stage functional form that composite nano fiber is 2.5mm to get diameter.
Embodiment 2
Ginseng continues to read preparation process shown in FIG. 1, and the present embodiment provides a kind of three-stage functional form degradable artificial ligaments
The preparation method of regeneration support, specifically comprises the following steps:
Step 1: Poly L-lactic acid (PLLA)/tussah silk fibroin (SF) that mass ratio is 3:1 is weighed on assay balance,
Stirring is heated under the conditions of 90 DEG C, must mix wire drawing molten liquid, it is spare；
Step 2: by fibroin albumen, being loaded with the mesoporous nano-grain for promoting tendon-bone healing growth factor, rush tendon-knitting growth
Three kinds of components of the factor are 80:16:4 dissolution according to mass ratio, prepare the casting liquid that mass-volume concentration is 20%；
Step 3: melt drawing molten liquid being subjected to melt drawing, it is compound long single to obtain the PLLA/SF that diameter is 150 μm
Silk；
Step 4: knitting technology will be combined to prepare internal diameter using 16 bursts of doublings, twisting after the compound long monofilament drawing of PLLA/SF
For the compound long monofilament braided fabric mechanics enhancement layer of PLLA/SF of the tubular structure of 4mm, long 8cm；
Step 5: the mechanics enhancement layer of tubular structure and the ePTFE mold of length 8cm being fitted together again, are placed in 70 DEG C
Cooling, the mechanics enhancement layer as artificial ligament are taken out in thermal finalization in drying box；
Step 6: after tubiform Textile Product is cooling, group is divided into fibroin albumen, is loaded with Deferoxamine mesoporous hydroxyapatite
The mass-volume concentration that nano particle, Deferoxamine mass ratio are 80:16:4 is that 32% casting liquid injects tubular knitted and mold
Complex in, be freeze-dried 24 hours, obtain first segment complex；
Step 7: again injecting the silk fibroin water solution that mass-volume concentration is 20% in first segment complex, freezing is dry
Dry 24 hours, obtain second segment complex；
Step 8: mesoporous hydroxyapatite nanoparticle, the Deferoxamine that group is finally divided into fibroin albumen, is loaded with Deferoxamine
The mass-volume concentration that mass ratio is 80:16:4 is to be freeze-dried 24 hours, obtain in 32% casting liquid injection second segment complex
Weave the three-stage solid cylindrical bracket reinforced；
Step 9: last columnar bracket outer surface sprays the PLLA/SF that one layer of mass ratio is 3:1 by electrostatic spinning technique
The degradable ligament regeneration support of three-stage functional form that composite nano fiber is 4.6mm to get diameter.
Specific embodiments of the present invention are described in detail above, but it is merely an example, the present invention is simultaneously unlimited
It is formed on particular embodiments described above.To those skilled in the art, any couple of present invention carries out equivalent modifications and
Substitution is also all among scope of the invention.Therefore, without departing from the spirit and scope of the invention made by equal transformation and
Modification, all should be contained within the scope of the invention.
Claims (10)
1. a kind of preparation method of three-stage functional form degradable artificial ligament regeneration support characterized by comprising
Step 1: being in mass ratio that 1:1~4:1 dissolution or hot melt are mixed with fibroin albumen by high-molecular organic material, obtain Compound spinning
Silk solution or mixing wire drawing molten liquid, it is spare；
Step 2: by fibroin albumen, being loaded with the mesoporous nano-grain for promoting tendon-bone healing growth factor, rush tendon-knitting growth factor
Three kinds of components are 75:20:5~95:4:1 hot melt mixing in mass ratio, prepare certain density uniformly mixed casting liquid；
Step 3: by the composite spinning solution of step 1) preparation or melt drawing molten liquid carries out spinning respectively or melt drawing obtains
To the nano-fibre yams or long monofilament of different-diameter and length；
Step 4: the nano-fibre yams of step 3) preparation or long monofilament are woven into tubiform Textile Product；
Step 5: tubiform Textile Product made from step 4) being covered in the mold of assembling, is integrally put into vacuum oven together with mold
It is dry to carry out thermal finalization, the mechanics enhancement layer as artificial ligament；
Step 6: the casting liquid injection step 5 that step 2) is prepared the cylindric mould with the tubiform Textile Product after thermal finalization in)
In tool, together with the processing of mold vacuum freeze drying, the first segment complex with mold is obtained, it is spare；
Step 7: certain density silk fibroin protein solution is continued to be cast to the cylinder that first segment complex is had in step 6) again
In shape mold, together with the processing of mold vacuum freeze drying, the first segment and second segment complex with mold are obtained, it is spare；
Step 8: the casting liquid that step 2) is prepared is continued to be cast to the circle that first segment and second segment complex are had in step 7)
In columnar mould, together with the processing of mold vacuum freeze drying, the three-stage bracket complex with mold is obtained, after taking off mold
It is spare；
Step 9: three-stage bracket complex prepared by step 8) being fixed on electrostatic spinning apparatus, electrostatic spinning technique is passed through
It spins on composite spinning solution described in the step 1) that thickness is about 0.01mm~5mm or mixes the nanofiber of wire drawing liquid, obtain
Three-stage functional form degradable artificial ligament regeneration support.
2. the preparation method of three-stage functional form degradable artificial ligament regeneration support according to claim 1, feature
Be, high-molecular organic material described in step 1) be selected from poly (lactic acid-glycolic acid) (PLGA), polydioxanone (PDO),
Polylactic acid (PLLA, PDLA, PDLLA), polycaprolactone (PCL), poly- (lactic acid-caprol acton) (PLCL), is gathered polyglycolic acid (PGA)
Hydroxy fatty acid (PHAs), polyester type biodegradable polyurethane, polyether-type Biodegradable polyurethane, poly- (ester-ether) type
One or more of Biodegradable polyurethane.
3. the preparation method of three-stage functional form degradable artificial ligament regeneration support according to claim 2, feature
Be, the mass/volume concentration (w/v) of composite spinning solution described in step 1) is 5%~20%, solvent be water, ethyl alcohol,
Dimethyl sulfoxide, Isosorbide-5-Nitrae-dioxane, hexafluoroisopropanol, n,N-Dimethylformamide, n,N-dimethylacetamide, tetrahydro furan
It mutters, one or more of methylene chloride, acetone, chloroform, trifluoroacetic acid, trifluoroethanol.
4. the preparation method of three-stage functional form degradable artificial ligament regeneration support according to claim 1, feature
It is, mesoporous nano-grain described in step 2) is that meso-porous nano silicon, meso-porous nano of the surface with hydroxyl, amino or carboxyl are raw
Object glass, meso-porous nano hydroxyl phosphorite, meso-porous nano bata-tricalcium phosphate, meso-porous nano attapulgite, meso-porous nano hydrotalcite,
One or more of meso-porous nano illite, meso-porous nano rectorite or meso-porous nano montmorillonite；The growth factor is
One or more of BMP-2, Deferoxamine, KGN, VEGF, dexamethasone, eugenol, chondroitin sulfate, interleukin.
5. the preparation method of three-stage functional form degradable artificial ligament regeneration support according to claim 4, feature
It is, the mesoporous nano-grain for being loaded with rush tendon-bone healing growth factor is the growth factor and the mesoporous nano-grain
Between by amidation process, ionic bonding reaction and physical absorption reaction one of or various ways be combined into.
6. the preparation method of three-stage functional form degradable artificial ligament regeneration support according to claim 1, feature
It is, the mass/volume concentration (w/v) for liquid of casting described in step 2) is 3.8%~52.4%.
7. the preparation method of three-stage functional form degradable artificial ligament regeneration support according to claim 1, feature
It is, nano-fibre yams described in step 3) are a diameter of 10 μm~1000 μ using the long monofilament as the composite yarn of core
m。
8. the preparation method of three-stage functional form degradable artificial ligament regeneration support according to claim 1, feature
It is, tubiform Textile Product internal diameter described in step 4) is 1mm~10mm, and length is 5cm~20cm.
9. the preparation method of three-stage functional form degradable artificial ligament regeneration support according to claim 1, feature
It is, heat setting temperature described in step 5) is 35 DEG C~90 DEG C；The mass/volume of silk fibroin protein solution described in step 7)
(w/v) concentration is 4.8%~34.6%.
10. a kind of three-stage functional form degradable artificial ligament such as any one of claim 1-9 the method preparation regenerates branch
Frame.
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