CN110376130B - Fluorescence spectrum detection system based on negative feedback adjustment - Google Patents
Fluorescence spectrum detection system based on negative feedback adjustment Download PDFInfo
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- CN110376130B CN110376130B CN201910704105.8A CN201910704105A CN110376130B CN 110376130 B CN110376130 B CN 110376130B CN 201910704105 A CN201910704105 A CN 201910704105A CN 110376130 B CN110376130 B CN 110376130B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0118—Apparatus with remote processing
- G01N2021/0125—Apparatus with remote processing with stored program or instructions
Abstract
The invention discloses a fluorescence spectrum detection system based on negative feedback regulation, which comprises: the device comprises a lower computer, a light source, an excitation monochromator, a beam splitter, a monitoring end photomultiplier, an amplifying circuit, a grating, a fluorescence detection area, an emission monochromator, a detection end photomultiplier and an upper computer; the lower computer controls the light source to emit laser, the same two paths of light are formed in the beam splitter after being screened by the exciting monochromator, one path of light is transmitted back to the lower computer through the photomultiplier at the monitoring end and the amplifying circuit, and the lower computer controls the light source to output to form negative feedback control; the other path of light is irradiated on a fluorescence detection area through a grating to be excited to generate fluorescence, the fluorescence passes through an emission monochromator and a detection end photomultiplier, the detection end photomultiplier carries out intensity detection, an electric signal is transmitted to a lower computer through an amplifying circuit, the lower computer transmits the detected and collected electric signal to an upper computer, and finally a complete fluorescence spectrogram is obtained on the upper computer.
Description
Technical Field
The invention relates to the technical field of spectrum detection, in particular to a fluorescence spectrum detection system based on negative feedback regulation.
Background
The fluorescence spectrum detection has the advantages of high sensitivity, strong selectivity, small sample consumption and the like, so the fluorescence spectrum detection has wide application in engineering application, such as monitoring of food safety in the food processing process, petroleum mineral exploration in geology, determination of soil mineral components, detection of trace elements in substances and the like.
At present, common fluorescence spectrum detection systems directly collect fluorescence excited by laser. In most assays, however, fluorescence detection is susceptible to interference from the external environment. Therefore, the stability of the fluorescence spectrum detection system largely determines the correctness and high precision of the fluorescence detection result.
Therefore, how to reduce the external interference during fluorescence detection and improve the stability of fluorescence spectrum detection is a problem that needs to be solved urgently by those skilled in the art.
Disclosure of Invention
In view of the above, the present invention provides a fluorescence spectrum detection system based on negative feedback adjustment,
in order to achieve the purpose, the invention adopts the following technical scheme:
a fluorescence spectrum detection system based on negative feedback regulation, comprising: the device comprises a lower computer, a light source, an excitation monochromator, a beam splitter, a monitoring end photomultiplier, an amplifying circuit, a grating, a fluorescence detection area, an emission monochromator, a detection end photomultiplier and an upper computer; the lower computer is connected with the light source and controls the light source to emit laser; the excitation monochromator and the beam splitter are sequentially arranged in the laser light path direction; the laser is divided into two paths at the beam splitter, and one path of the laser irradiates the photomultiplier at the monitoring end; the other path of laser irradiates the fluorescence detection area through the grating; the fluorescence detection area generates fluorescence, and the emission monochromator and the detection end photomultiplier are sequentially arranged in the fluorescence light path direction; the amplifying circuit is connected with the photomultiplier at the monitoring end and the photomultiplier at the detection end, and is connected with the lower computer through a lead; and the lower computer is connected with the upper computer for communication.
Preferably, the light source emits laser to the excitation monochromator, the excitation monochromator screens the laser, the screened laser irradiates to the beam splitter, the beam splitter generates two paths of laser with completely consistent light intensity and wavelength, the laser enters the monitoring end photomultiplier to be converted into current, the current is transmitted to the lower computer through the amplifying circuit, the other path of light irradiates to the fluorescence detection area through the grating, the fluorescence passes through the emission monochromator, the detection end photomultiplier is converted into the current, and the current is transmitted to the lower computer through the amplifying circuit.
Preferably, the photomultiplier at the monitoring end transmits the electric signal to the amplifying circuit, the amplifying circuit converts the acquired weak current into a voltage signal which can be acquired by the lower computer and transmits the voltage signal to the lower computer, and the lower computer dynamically keeps the lower computer outputting constant current to control the intensity of the light source according to the voltage signal by adopting a PID algorithm.
Preferably, the lower computer, the light source, the excitation monochromator, the beam splitter, the monitoring end photomultiplier and the amplifying circuit form a light intensity feedback system, the light intensity feedback system is in communication with the lower computer through photoelectric conversion, the lower computer collects the voltage signal of the amplifying circuit, fits the voltage signal to obtain system light intensity, and compares the system light intensity with an original set signal of the lower computer; if the light intensity of the system is larger, the lower computer controls to reduce the driving current output to the light source; otherwise, increasing the driving current output to the light source; the light intensity feedback system adopts the PID algorithm in the feedback adjustment process.
Preferably, the fluorescence passes through the emission monochromator, and the emission monochromator changes the wavelength of the fluorescence passing through the emission monochromator to obtain narrow-wave fluorescence; the narrow-wave fluorescence is transmitted to the photomultiplier at the detection end to be converted into the current, and the current is converted into the voltage signal through the amplifying circuit and is transmitted to the lower computer; the lower computer records the voltage signal corresponding to the narrow-wave fluorescence and transmits the voltage signal to the upper computer, and the upper computer forms a fluorescence spectrogram according to the voltage signal and the narrow-wave fluorescence corresponding to the voltage signal.
Preferably, the lower computer transmits data to the upper computer through the usb.
According to the technical scheme, compared with the prior art, the fluorescence spectrum detection system based on negative feedback regulation is disclosed in the invention, a lower computer sends a signal to control a light source to emit laser, the laser is transmitted to an excitation monochromator, the same two paths of light rays are formed in a beam splitter after being screened by the excitation monochromator, one path of laser is converted into an electric signal through a photomultiplier at a monitoring end and is transmitted to an amplifying circuit, the amplifying circuit processes the electric signal and transmits the electric signal to the lower computer, and the lower computer processes the electric signal to control the output of the light source, so that negative feedback control is formed; the other path of laser is irradiated on a fluorescence detection area through a grating to excite and generate fluorescence, the fluorescence is transmitted to a detection end photomultiplier after being screened by an emission monochromator, the detection end photomultiplier performs photoelectric conversion on the fluorescence and performs intensity detection, an intensity detection result is transmitted to a lower computer through an amplifying circuit, the lower computer transmits an electric signal acquired through detection to an upper computer, and finally a complete fluorescence spectrogram is obtained on the upper computer.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a schematic diagram of a fluorescence spectrum detection system based on negative feedback regulation according to the present invention;
FIG. 2 is a schematic diagram of the negative feedback process provided by the present invention.
1-a lower computer, 2-a light source, 3-an excitation monochromator, 4-a beam splitter, 5-a monitoring end photomultiplier, 6-an amplifying circuit, 7-a grating, 8-a fluorescence detection area, 9-an emission monochromator, 10-a detection end photomultiplier and 11-an upper computer.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the invention discloses a fluorescence spectrum detection system based on negative feedback regulation, which comprises: the device comprises a lower computer 1, a light source 2, an excitation monochromator 3, a beam splitter 4, a monitoring end photomultiplier 5, an amplifying circuit 6, a grating 7, a fluorescence detection area 8, an emission monochromator 9, a detection end photomultiplier 10 and an upper computer 11; the lower computer 1 is connected with the light source 2 and controls the light source to emit laser; an excitation monochromator 3 and a beam splitter 4 are sequentially arranged according to the laser light path direction; the laser is divided into two paths at the beam splitter, and one path of laser irradiates to the photomultiplier 10 at the monitoring end; the other path of laser irradiates a fluorescence detection area 8 through a grating 7; the fluorescence detection area generates fluorescence, and an emission monochromator 9 and a detection end photomultiplier 10 are sequentially arranged in the fluorescence light path direction; the amplifying circuit 6 is connected with the monitoring end photomultiplier 5 and the detection end photomultiplier 10 and is connected with the lower computer 1 through a lead; the lower computer 1 is connected with the upper computer 11 for communication.
In order to further optimize the technical scheme, the light source 2 emits laser to the excitation monochromator 3, the excitation monochromator 3 screens the laser, the screened laser irradiates to the beam splitter 4, the beam splitter 4 generates two paths of laser with completely consistent light intensity and wavelength, one path of laser enters the photomultiplier 5 at the monitoring end to be converted into current, the current is transmitted to the lower computer 1 through the amplifying circuit 6, the other path of light irradiates to the fluorescence detection area 8 through the grating 7 to generate fluorescence, the fluorescence is converted into current through the photomultiplier 10 at the detection end through the emission monochromator 9, and then the current is transmitted to the lower computer 1 through the amplifying circuit 6.
In order to further optimize the technical scheme, the photomultiplier 5 at the monitoring end transmits current to the amplifying circuit 6, the amplifying circuit 6 converts the collected weak current into a voltage signal and transmits the voltage signal to the lower computer 1, and the lower computer 1 dynamically keeps the constant current output by the lower computer 1 according to the voltage signal by adopting a PID algorithm to control the light intensity output by the light source 2. The weak electric signal is amplified by the amplifying circuit, so that the accurate acquisition of the laser signal is realized, and the light source is accurately controlled to generate laser according to the voltage signal result.
In order to further optimize the technical scheme, the lower computer 1, the light source 2, the exciting monochromator 3, the beam splitter 4, the monitoring end photomultiplier 5 and the amplifying circuit 6 form a light intensity feedback system, the light intensity feedback system is communicated with the lower computer 1 through photoelectric conversion, the lower computer 1 collects voltage signals of the amplifying circuit 6, the voltage signals are fitted to obtain system light intensity, and the system light intensity is compared with original set signals of the lower computer 1; if the light intensity of the system is larger, the driving current output to the light source 2 is controlled and reduced by the lower computer 1; on the contrary, the driving current output to the light source 2 is increased; the light intensity feedback system adopts a PID dynamic balance method in the feedback adjustment process.
In order to further optimize the technical scheme, the fluorescence passes through the emission monochromator 9, the emission monochromator 9 screens the fluorescence to obtain the fluorescence with different wavelengths, the fluorescence with the original wider wavelength range is changed into the fluorescence with the narrower wavelength range, the emission monochromator 9 sequentially changes the wavelength of the passable fluorescence from short to long, meanwhile, the photomultiplier tube 10 at the detection end converts the passable fluorescence into current, the current is changed into a voltage signal which can be collected by the lower computer 1 through the amplifying circuit 6, and the voltage signal is collected by the lower computer 1 and then recorded. The emission monochromator 9 increases the wavelength of the passing fluorescence, the above processes are repeated, the photomultiplier 10 at the detection end receives the fluorescence with different wavelengths and generates corresponding currents, after all the acquisition processes are finished, the lower computer 1 sends all the data to the upper computer 11, and the fluorescence spectrograms of the fluorescence intensity represented by the fluorescence with different wavelengths and corresponding voltage signals are obtained in the upper computer 11.
In order to further optimize the above technical solution, the lower computer 1 transmits data to the upper computer 11 through the usb.
Examples
FIG. 1 shows the working principle of a fluorescence spectrum detection system based on negative feedback regulation: firstly, a DAC signal is sent by a lower computer 1 to control a light source 2 to output a constant current, laser sent by the light source 2 is screened by an exciting monochromator 3 to generate narrow-wavelength light, the narrow-wavelength light forms two same light beams through a beam splitter 4, one light beam is converted into an electric signal through a monitoring end photomultiplier 5 and is transmitted to an amplifying circuit 6, the electric signal is finally transmitted back to the lower computer 1 through the amplifying circuit 6, the electric signal transmitted by the amplifier circuit is processed by the lower computer 1 to control the light source 2 to output, and negative feedback control is formed; the other path of light irradiates on a fluorescence detection area 8 through a grating 7, the grating is used for controlling the irradiation duration of the monochromatic light, the monochromatic light irradiates on the fluorescence detection area 8 to be excited to generate fluorescence, the generated fluorescence is transmitted to a detection end photomultiplier 10 through an emission monochromator 9, the emission monochromator 9 screens the fluorescence with different wavelengths, the fluorescence is converted into an electric signal when passing through the detection end photomultiplier 10, the intensity of the electric signal is detected, finally the electric signal is transmitted to a lower computer 1 through an amplifying circuit 6, the lower computer 1 transmits the electric signal for detecting the collected fluorescence intensity to an upper computer, and finally a complete fluorescence spectrogram is obtained by the upper computer 11.
Fig. 2 shows a light intensity feedback system: firstly, a lower computer 1 sends out a preset signal, a light source 2 is controlled to generate laser, and the laser wavelength range is smaller and the monochromator 3 is excited, so that the monochromator is stronger in monochromaticity. The laser is divided into two paths of light with completely consistent light intensity and wavelength through the beam splitter 4, one path of laser is converted into an electric signal through the monitoring end photomultiplier 5 and is fed back to the lower computer 1 through the amplifying circuit 6, the lower computer 1 continuously adjusts the output current of the light source by adopting a PID algorithm so that the output laser keeps constant, and after the system is kept stable, the grating 7 is opened to irradiate the other path of laser to the fluorescence detection area 8 so as to realize fluorescence spectrum detection.
The invention has the beneficial effects that:
1. a photomultiplier at a monitoring end is used for forming a negative feedback system, so that the constancy of the light intensity of output laser is ensured, and the anti-interference performance of the system is enhanced;
2. the emission monochromator screens the fluorescence with different wavelengths to obtain corresponding light intensity, and the intensity is measured in a narrow wavelength range to prevent the interference of the fluorescence with other wavelengths, thereby greatly improving the accuracy of the fluorescence spectrum.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (4)
1. A fluorescence spectrum detection system based on negative feedback regulation, comprising: the device comprises a lower computer (1), a light source (2), an excitation monochromator (3), a beam splitter (4), a monitoring end photomultiplier (5), an amplifying circuit (6), a grating (7), a fluorescence detection area (8), an emission monochromator (9), a detection end photomultiplier (10) and an upper computer (11); the lower computer (1) is connected with the light source (2) and controls the light source to emit laser; the excitation monochromator (3) and the beam splitter (4) are sequentially arranged in the laser light path direction; the laser is divided into two paths at the beam splitter, and one path of the laser irradiates the photomultiplier (10) at the monitoring end; the other path of laser irradiates the fluorescence detection area (8) through the grating (7); the fluorescence detection area generates fluorescence, and the emission monochromator (9) and the detection end photomultiplier (10) are sequentially arranged in the fluorescence light path direction;
the amplifying circuit (6) is connected with the monitoring end photomultiplier (5) and the detection end photomultiplier (10) and is connected with the lower computer (1) through a lead; the lower computer (1) is connected with the upper computer (11) for communication;
the light source (2) emits laser to the excitation monochromator (3), the excitation monochromator (3) screens the laser, the screened laser irradiates the beam splitter (4), the beam splitter generates two paths of laser with completely consistent light intensity and wavelength, one path of laser enters the monitoring end photomultiplier (5) to be converted into current and is transmitted to the lower computer (1) through the amplifying circuit (6), the other path of laser irradiates the fluorescence detection area (8) through the grating (7) to generate fluorescence, the fluorescence passes through the emission monochromator (9), is converted into the current at the detection end photomultiplier (10), and is transmitted to the lower computer (1) through the amplifying circuit (6); the grating (7) is used for controlling the irradiation duration of the monochromatic light;
the lower computer (1), the light source (2), the excitation monochromator (3), the beam splitter (4), the monitoring end photomultiplier (5) and the amplifying circuit (6) form a light intensity feedback system, the light intensity feedback system is communicated with the lower computer (1) through photoelectric conversion, the lower computer (1) collects a voltage signal of the amplifying circuit (6), fits the voltage signal to obtain system light intensity, and compares the system light intensity with an original set signal of the lower computer (1); if the light intensity of the system is larger, the driving current output to the light source (2) is controlled and reduced by the lower computer (1); otherwise, increasing the driving current output to the light source (2); the light intensity feedback system adopts a PID algorithm in the feedback adjustment process; the lower computer (1) continuously adjusts the output current of the light source by adopting a PID algorithm so that the output laser keeps constant, and after the system keeps stable, the grating (7) is opened to irradiate the other path of laser to the fluorescence detection area (8) so as to realize fluorescence spectrum detection.
2. The fluorescence spectrum detection system based on negative feedback regulation according to claim 1, wherein the photomultiplier (5) at the monitoring end transmits the current to the amplification circuit (6), the amplification circuit (6) converts the received current into a voltage signal and transmits the voltage signal to the lower computer (1), and the lower computer (1) dynamically keeps the lower computer (1) to output a constant current to control the intensity of the light source (2) according to the collected voltage signal by adopting a PID algorithm.
3. The fluorescence spectrum detection system based on negative feedback regulation of claim 1, wherein the fluorescence passes through the emission monochromator (9), and the emission monochromator (9) changes the wavelength of the passed fluorescence to obtain narrow-wave fluorescence; the narrow-wave fluorescence is transmitted to the photomultiplier (10) at the detection end to be converted into the current, and the current is converted into the voltage signal through the amplifying circuit (6) and transmitted to the lower computer (1); the lower computer (1) records the voltage signal of the narrow-wave fluorescence and transmits the voltage signal to the upper computer (11), and the upper computer (11) forms a fluorescence spectrogram according to the voltage signal and the narrow-wave fluorescence corresponding to the voltage signal.
4. The fluorescence spectrum detection system based on negative feedback regulation of claim 1, wherein the lower computer (1) transmits data to the upper computer (11) through usb.
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