CN110367242A - Apoptotic body saves the store method of liquid and apoptotic body - Google Patents
Apoptotic body saves the store method of liquid and apoptotic body Download PDFInfo
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- CN110367242A CN110367242A CN201910711147.4A CN201910711147A CN110367242A CN 110367242 A CN110367242 A CN 110367242A CN 201910711147 A CN201910711147 A CN 201910711147A CN 110367242 A CN110367242 A CN 110367242A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
Abstract
The present invention provides a kind of apoptotic body freezen protective liquid and the external long-term preservation method of apoptotic body, freezen protective liquid provided by the invention includes following components: cryoprotector, antioxidant and buffer.According to the technical solution of the present invention, stable apoptotic body source can be provided for stem-cell therapy, the conversion of beauty industry.
Description
Technical field
The invention belongs to apoptotic body technical fields more particularly to a kind of mescenchymal stem cell apoptotic body to protect for a long time in vitro
The store method of the preservation liquid and apoptotic body deposited.
Background technique
Mescenchymal stem cell is a research branch for stem cell, and stem cell is a kind of with self-replacation and Multidirectional Differentiation
The cell of ability, they can constantly self-renewing, and be transformed into one or more composition human body groups under given conditions
It knits or the cell of organ.Stem cell is the cells of origin of body, is the progenitor cell to form the various histoorgans of human body.
The clinical research of mescenchymal stem cell is carried out in many countries, and the U.S. has approved 60 remainder clinical tests, with
Mescenchymal stem cell and its relevant technologies it is increasingly mature, China also has approved multinomial clinical test, and it is dry to have entered into mesenchyma
The stage of cell core technical research.The development of stem-cell research work, including the high match cell of country are reinforced energetically in China
More authoritative research institutions and each place umbilical cord including genetic engineering Co., Ltd, cell products National Engineering Research Centre
Into clinic is guided investigative technique by blood bank.It is used to treat the Therapy study of more than ten refractory diseases for mescenchymal stem cell,
In addition to being used to promote to restore hematopoiesis, is improved other than leukaemia and refractory anemia etc. with candidate stem cell co-transplantation, be also used to the heart
Cranial vascular disease, cirrhosis, bone and muscle degenerative disease, brain and neurologic defict, senile dementia and lupus erythematosus and hard
The Therapy study of the autoimmune diseases such as skin disease, the partial clinical test result having been achieved with are encouraging.
The chromatinal mass (fragment) that apoptotic cell is formed through karyorrhexis, then entire cell passes through the sides such as germination, blistering
Formula formed a spherical protrusion, and its root strangulation fall off to be formed it is some differ in size, include cytoplasm, organelle and core
The corpusculum of fragment is known as apoptotic body.
The formation of apoptotic body can be by two ways first is that passing through the mechanism that falls off of germinateing: the dye assembled in apoptotic cell
Chromaticness block forms the chromatinal mass that differs in size through karyorrhexis, and then entire cell forms one by the modes such as sprout, blister
Spherical film packet corpusculum, includes cytoplasm, organelle and fragment, falls off to form apoptotic body.Second is that forming machine by autophagosome
System: the organelles such as mitochondria, endoplasmic reticulum and other cytoplasmic components are wrapped to form autophagosome by endoplasmic reticulum together in apoptotic cell,
After merging with apoptotic cell film, autophagosome discharge extracellularly becomes apoptotic body.
The patent of the application aspect application number CN201580039370.9 of apoptotic body is illustrated at present through retrieval discovery
The therapeutic treatment application of apoptotic body and its separation method, but the preservation of apoptotic body and application mode are not carried out
It illustrates, the prior art also without the long-term preservation method of open apoptotic body, is not easy to extensive industrial application.
Summary of the invention
The object of the present invention is to provide the external long-term preservation sides that a kind of apoptotic body saves liquid and apoptotic body
Method can be according to the technical solution of the present invention stem-cell therapy, and the conversion of beauty industry provides steadily apoptotic body source.
The purpose of the present invention is achieved through the following technical solutions:
First aspect present invention provides a kind of freezen protective liquid of apoptotic body, includes following components: cryoprotector, antioxygen
Agent and buffer.
Preferably, the cryoprotector is selected from hydroxyethyl starch, glycerol, human serum albumins and trehalose.
Preferably, the antioxidant is selected from vitamin C.
Preferably, the buffer is selected from amino acid injection, glucose injection, physiological saline or sodium lactate ringer's note
Penetrate one of liquid or a variety of.
Second aspect of the present invention, provide a kind of apoptotic body freezen protective liquid includes according to parts by weight: ethoxy forms sediment
5-10 parts of powder, 5 parts of glycerol, 10-20 parts of human serum albumins, 5 parts of trehalose, 0.1-1 parts of vitamin C injection, amino acid note
Penetrate 5-10 parts of liquid, 1-5 parts of glucose injection and 45-65 parts of physiological saline.
Preferably, the parts by weight of the hydroxyethyl starch are 5 parts, 6 parts, 7 parts, 8 parts, 9 parts or 10 parts.
Preferably, the parts by weight of the human serum albumins are 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16
Part, 17 parts, 18 parts, 19 parts or 20 parts.
Preferably, the parts by weight of the vitamin C injection are 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6
Part, 0.7 part, 0.8 part, 0.9 part or 1 part.
Preferably, the parts by weight of the amino acid injection are 5 parts, 6 parts, 7 parts, 8 parts, 9 parts or 10 parts.
Preferably, the parts by weight of the glucose injection are 1 part, 2 parts, 3 parts, 4 or 5 parts.
Preferably, the parts by weight of the physiological saline are 45 parts, 46 parts, 47 parts, 48 parts, 49 parts, 50 parts, 51 parts, 52
Part, 53 parts, 54 parts, 55 parts
Third aspect present invention, provide a kind of apoptotic body freezen protective liquid includes according to parts by weight: hydroxyethyl starch 5-
10 parts, 5 parts of glycerol, 10-20 parts of human serum albumins, 5 parts of trehalose, 0.1-1 parts of vitamin C injection, amino acid injection
5-10 parts, 1-5 parts of glucose injection and 45-65 parts of sodium lactate ringer's injection.
Preferably, the parts by weight of the hydroxyethyl starch are 5 parts, 6 parts, 7 parts, 8 parts, 9 parts or 10 parts.
Preferably, the parts by weight of the human serum albumins are 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16
Part, 17 parts, 18 parts, 19 parts or 20 parts.
Preferably, the parts by weight of the vitamin C injection are 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6
Part, 0.7 part, 0.8 part, 0.9 part or 1 part.
Preferably, the parts by weight of the amino acid injection are 5 parts, 6 parts, 7 parts, 8 parts, 9 parts or 10 parts.
Preferably, the parts by weight of the glucose injection are 1 part, 2 parts, 3 parts, 4 or 5 parts.
Preferably, the parts by weight of the sodium lactate ringer's injection be 45 parts, 46 parts, 47 parts, 48 parts, 49 parts, 50 parts,
51 parts, 52 parts, 53 parts, 54 parts, 55 parts, 56 parts, 57 parts, 58 parts, 59 parts, 60 parts, 61 parts, 62 parts, 63 parts, 64 parts or 65 parts.
Preferably, the apoptotic body is mescenchymal stem cell apoptotic body or apoptosis of mesenchymal stem cell corpusculum.
Fourth aspect present invention provides a kind of store method of apoptotic body, comprising the following steps:
Apoptotic body is obtained, the freezen protective liquid is added in the apoptotic body, is mixed, obtains and saves sample；By the guarantor
Storage sample this addition cryopreservation tube is placed in Programmed cryopreservation box, is saved 24 hours in -80 degree refrigerators；The preservation sample is shifted
It is saved into gas phase and liquid phase holding vessel, it is spare.
Preferably, the volume ratio of the apoptotic body and the freezen protective liquid is 1:(30 ~ 100).
Preferably, the volume ratio of the apoptotic body and the freezen protective liquid is 1:30,1:40,1:50,1:60,1:
70,1:80,1:90 or 1:100.
Preferably, the freezen protective liquid is above-mentioned freezen protective liquid；Apoptotic body of the present invention is by with lower section
Method is prepared:
(1) umbilical cord tissue is obtained, jelly of Wharton is removed, is added in culture medium after shredding and carries out primitive cell culture；
(2) it carries out half amount within the cell of above-mentioned culture the 5th day after culture and changes liquid；
(3) it carries out within hereafter cell every 3 days changing when liquid to plating cells rate is about 80% and be passed on；
(4) extraction that apoptotic body is carried out when cell is passaged to P5 by step (3) (cellular morphology is shown in Fig. 1) is repeated；
(5) acquisition of apoptotic body is carried out using the starvation method of the prior art.
Compared with prior art, the beneficial effects of the present invention are:
Freezen protective liquid provided by the invention is compounded using cryoprotector, antioxidant and buffer, small for carrying out apoptosis
The storage in vitro of body, the structure of apoptotic body and its active storage rate are 95% or more after saving, great convenience apoptosis
Corpusculum industrial application.
Further, freezen protective liquid provided by the invention specifically uses hydroxyethyl starch 5-10 parts, 5 parts of glycerol, people's blood
- 20 parts of pure protein 10,5 parts of trehalose, 0.1-1 parts of vitamin C injection, 5-10 parts of amino acid injection, glucose injection
1-5 parts and sodium lactate ringer's injection 45-65 parts of liquid are compounded, and said components are useful clinically product, proportion compatibility
It is to be crossed by lot of experiment validation, that is, is able to satisfy the needs for freezing and recovering of apoptotic body, and safety with higher
Property, the later period reduces the removal of impurity, is conducive to the application of the efficient quick of product when applying, can be applied to disease treatment biology
Preparation, beauty product, health product.
Hydroxyethyl starch (Hetastarch) is present clinically widely used artificial synthesized colloidal solution, while
It is a kind of natural polysaccharide.Hydroxyethyl starch (HES) is that the glucose ring of amylopectin in corn or potato is formed through hydroxyethylation
Polymer composite.Native starch cannot be used as blood substitutes, because native starch property is unstable and easily endogenous
Property amylorrhexis.Starch can delay decomposition and elimination in blood after hydroxyethylation, significantly extend it in blood vessel
The interior residence time.HES has the function of expanding blood volume, and healthy volunteer is transfused after HES1000ml 10 minutes, blood volume compared with
Averagely increase 900ml before infusion, 415ml is reduced to after 6 hours, also keeps 285ml after 24 hours.
Glycerol has good shrink effect as a kind of good moisturizer, can be to avoid apoptotic body in freezing item
Apoptotic body is destroyed because of water crystallization under part.
Human serum albumins can be with transport of fatty acids, BILE PIGMENTS, amino acid, steroid hormone, metal ion in body fluid
With many treatment molecules etc.: while maintaining the normal osmotic pressure of blood.Clinically human serum albumins can be used for treating shock
With burn, for supplementing because of operation, contingency or big bleeding caused by blood loss, can also be used as plasma extender.?
Human serum albumins can maintain the osmotic pressure of apoptotic body in the present invention.
Trehalose has magical protective effect to organism, because trehalose is in high temperature, high and cold, hyperosmosis and drying
Unique protective film can be formed in cell surface under the severe environmental conditions such as dehydration, is effectively protected the mistake of protein molecule invariance
It is living, thus the life process and biological characteristic of the body that sustains life.
Vitamin C has the effect of anti-oxidant, free radical resisting.
Amino acid injection is used to supplement amino acid for apoptotic body and maintains the nutritional need of apoptotic body.
Glucose injection is used to supplement the energy requirement of apoptotic body.
Sodium lactate ringer's injection is used to adjust the electrolyte and acid-base balance medicine of culture solution.
The storage in vitro that apoptotic body is carried out by the way of freezing, apoptotic body after saving are put forward for the first time in the present invention
Structure and its active storage rate 95% or more, great convenience apoptotic body industrial application.
Detailed description of the invention
Fig. 1 umbilical cord mesenchymal stem cells morphology photo；
Fig. 2 is the influence of apoptotic body cell proliferation rate under damaging condition in umbilical cord mesenchymal stem cells source；
Fig. 3 is mesenchymal stem cell morphology photo；
Fig. 4 is the influence of apoptotic body cell proliferation rate under damaging condition in mesenchymal stem cell source.
Specific embodiment
With reference to the accompanying drawings and examples, the content of present invention is described in further details.
Embodiment 1
The present embodiment provides a kind of apoptotic body freezen protective liquid, and according to parts by weight, the freezen protective liquid includes with the following group
Point: 5 parts of hydroxyethyl starch, 5 parts of glycerol, 20 parts of human serum albumins, 5 parts of trehalose, 1 part of vc injection, amino acid injection
10 parts, 5 parts of glucose injection and 49 parts of physiological saline.
Embodiment 2
The present embodiment provides a kind of apoptotic body freezen protective liquid, and according to parts by weight, the freezen protective liquid includes with the following group
Point: 6 parts of hydroxyethyl starch, 5 parts of glycerol, 10 parts of human serum albumins, 5 parts of trehalose, 0.1 part of vitamin C injection, amino acid
5 parts of injection, 1 part of glucose injection and 45 parts of sodium lactate ringer's injection.
Embodiment 3
The present embodiment provides a kind of apoptotic body freezen protective liquid, and according to parts by weight, the freezen protective liquid includes with the following group
Point: 7 parts of hydroxyethyl starch, 5 parts of glycerol, 12 parts of human serum albumins, 5 parts of trehalose, 0.2 part of vitamin C injection, amino acid
6 parts of injection, 2 parts of glucose injection and 46 parts of sodium lactate ringer's injection.
Embodiment 4
The present embodiment provides a kind of apoptotic body freezen protective liquid, and according to parts by weight, the freezen protective liquid includes with the following group
Point: 8 parts of hydroxyethyl starch, 5 parts of glycerol, 12 parts of human serum albumins, 5 parts of trehalose, 0.3 part of vitamin C injection, amino acid
7 parts of injection, 3 parts of glucose injection and 50 parts of physiological saline.
Embodiment 5
The present embodiment provides a kind of apoptotic body freezen protective liquid, and according to parts by weight, the freezen protective liquid includes with the following group
Point: 9 parts of hydroxyethyl starch, 5 parts of glycerol, 12 parts of human serum albumins, 5 parts of trehalose, 0.5 part of vitamin C injection, amino acid
8 parts of injection, 4 parts of glucose injection and 55 parts of sodium lactate ringer's injection.
Embodiment 6
The present embodiment provides a kind of apoptotic body freezen protective liquid, and according to parts by weight, the freezen protective liquid includes with the following group
Point: 10 parts of hydroxyethyl starch, 5 parts of glycerol, 15 parts of human serum albumins, 5 parts of trehalose, 0.7 part of vitamin C injection, amino
9 parts of acid injection, 4 parts of glucose injection and 60 parts of sodium lactate ringer's injection.
Embodiment 7
The present embodiment provides a kind of apoptotic body freezen protective liquid, and according to parts by weight, the freezen protective liquid includes with the following group
Point: 10 parts of hydroxyethyl starch, 5 parts of glycerol, 18 parts of human serum albumins, 5 parts of trehalose, 0.9 part of vitamin C injection, amino
10 parts of acid injection, 5 parts of glucose injection and 65 parts of sodium lactate ringer's injection.
Embodiment 8
The present embodiment provides a kind of methods of umbilical cord mesenchymal stem cells apoptotic body freezen protective, comprising the following steps:
Umbilical cord mesenchymal stem cells apoptotic body is obtained, 1 institute of embodiment is added in the umbilical cord mesenchymal stem cells apoptotic body
It states in freezen protective liquid, mixes, obtain and save sample；Preservation sample addition cryopreservation tube is placed on Programmed cryopreservation box
In, it is saved 24 hours in -80 degree refrigerators；The preservation sample is transferred in gas phase and liquid phase holding vessel and is saved, it is spare.
Umbilical cord mesenchymal stem cells apoptotic body described in the present embodiment is prepared by following methods:
(1) umbilical cord tissue is obtained, jelly of Wharton is removed, is added in culture medium and is cultivated after shredding；
(2) it carries out half amount within the cell of above-mentioned culture the 5th day after culture and changes liquid；
(3) it carries out within hereafter cell every 3 days changing when liquid to plating cells rate is about 80% and be passed on；
(4) extraction that apoptotic body is carried out when cell is passaged to P5 by step (3) is repeated, the cellular morphology in P5 generation is shown in Fig. 3；
(5) acquisition of apoptotic body is carried out using the starvation method of the prior art.
Embodiment 9
The present embodiment provides a kind of methods of apoptosis of mesenchymal stem cell corpusculum freezen protective, comprising the following steps:
Apoptosis of mesenchymal stem cell corpusculum is obtained, 7 institute of embodiment is added in the apoptosis of mesenchymal stem cell corpusculum
It states in freezen protective liquid, mixes, obtain and save sample；Preservation sample addition cryopreservation tube is placed on Programmed cryopreservation box
In, it is saved 24 hours in -80 degree refrigerators；The preservation sample is transferred in gas phase and liquid phase holding vessel and is saved, it is spare.
Apoptosis of mesenchymal stem cell corpusculum described in the present embodiment is prepared by following methods:
(1) syringe extracts rat marrow, is carried out after washing 3 three times is resuspended using PBS, is added after culture solution is resuspended and culture is added
It is cultivated in bottle, changes liquid after cell is adherent, passed on when cell Proliferation is to 80%；
(2) cell be passaged to P5 for when carry out the separation of apoptotic body, the cellular morphology in P5 generation is shown in Fig. 3；
(3) it carries out within hereafter cell every 3 days changing when liquid to plating cells rate is about 80% and be passed on；
(4) acquisition that apoptotic body is carried out using thermal stress method in the prior art is directly frozen part apoptotic body in -80 degree
In refrigerator.
10 compliance test result of embodiment
Freshly extd umbilical cord mesenchymal stem cells apoptotic body is taken, as A group；Between the umbilical cord for taking the embodiment of the present invention 8 to freeze
Mesenchymal stem cells apoptotic body is recovered, the apoptotic body after being recovered, as B group.It is added respectively into A group and B group
Cell culture is carried out after cell culture fluid, cell culture to 60% or so is irradiated after five minutes using UVA, is continued thin
The culture of born of the same parents, culture carried out cell count after 2 days, and count results are as shown in Figure 2.As can be seen that being mentioned using the embodiment of the present invention
The freezen protective liquid and freezing method of confession carry out freezen protective to apoptotic body, the structure and its activity of apoptotic body after saving
Storage rate 95% or more, this provides convenience for the industrial application of apoptotic body.
11 compliance test result of embodiment
Freshly extd apoptosis of mesenchymal stem cell corpusculum is taken, as C group；The apoptosis for taking the embodiment of the present invention 9 to freeze is small
Body is recovered, the apoptotic body after being recovered, as D group.It is carried out after cell culture fluid is added into C group and D group respectively
Cell culture, cell culture to 60% or so are irradiated the culture for continuing cell after five minutes using UVA, cultivate 2 days
After carry out cell count, count results are as shown in Figure 4.As can be seen that using freezen protective liquid provided in an embodiment of the present invention and
Freezing method carries out freezen protective to apoptosis of mesenchymal stem cell corpusculum, and apoptosis of mesenchymal stem cell is small after saving
95% or more, this provides convenience for the industrial application of apoptotic body for the structure of body and its active storage rate.
Claims (10)
1. apoptotic body freezen protective liquid, which is characterized in that include following components: cryoprotector, antioxidant and buffer.
2. freezen protective liquid according to claim 1, which is characterized in that the cryoprotector be selected from hydroxyethyl starch,
Glycerol, human serum albumins and trehalose.
3. freezen protective liquid according to claim 1, which is characterized in that the antioxidant is selected from vitamin C.
4. freezen protective liquid according to claim 1, which is characterized in that the buffer is selected from amino acid injection, Portugal
One of grape sugar injection, physiological saline or sodium lactate ringer's injection are a variety of.
5. apoptotic body freezen protective liquid, which is characterized in that according to parts by weight, include: 5-10 parts of hydroxyethyl starch, glycerol 5
Part, 10-20 parts of human serum albumins, 5 parts of trehalose, 0.1-1 parts of vitamin C injection, 5-10 parts of amino acid injection, grape
Sugared injection 1-5 parts and physiological saline 45-65 parts.
6. apoptotic body freezen protective liquid, which is characterized in that according to parts by weight, include: 5-10 parts of hydroxyethyl starch, glycerol 5
Part, 10-20 parts of human serum albumins, 5 parts of trehalose, 0.1-1 parts of vitamin C injection, 5-10 parts of amino acid injection, grape
Sugared injection 1-5 parts and sodium lactate ringer's injection 45-65 parts.
7. freezen protective liquid according to claim 5 or 6, which is characterized in that the apoptotic body is mescenchymal stem cell
Apoptotic body or apoptosis of mesenchymal stem cell corpusculum.
8. a kind of store method of apoptotic body, which comprises the following steps:
Apoptotic body is obtained, the apoptotic body is added in the freezen protective liquid, is mixed, obtains and saves sample；It will be described
It saves sample addition cryopreservation tube to be placed in Programmed cryopreservation box, be saved 24 hours in -80 degree refrigerators；The preservation sample is turned
It moves in gas phase and liquid phase holding vessel and saves, it is spare.
9. according to the method described in claim 8, it is characterized in that, the volume ratio of the apoptotic body and the freezen protective liquid
For 1:(30 ~ 100).
10. according to the method described in claim 8, it is characterized in that, the preservation liquid is that claim 1-4 is described in any item
Freezen protective liquid.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110946129A (en) * | 2019-11-08 | 2020-04-03 | 浙江卫未生物医药科技有限公司 | High-survival-rate frozen stock solution after cell recovery |
| CN111587877A (en) * | 2020-06-28 | 2020-08-28 | 上海交通大学医学院附属第九人民医院 | Stem cell cryopreservation protective agent, preparation method and application |
| CN112715533A (en) * | 2021-02-26 | 2021-04-30 | 康妍葆(北京)干细胞科技有限公司 | Cryopreservation solution and cryopreservation method for mesenchymal stem cells |
| CN112806355A (en) * | 2021-01-20 | 2021-05-18 | 山东科硕生物技术有限公司 | Cell preservation solution and preparation method and application thereof |
| WO2021190620A1 (en) * | 2020-03-27 | 2021-09-30 | 上海我武干细胞科技有限公司 | Cell preservation fluid and kit thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101351118A (en) * | 2005-11-02 | 2009-01-21 | 特拉科斯有限公司 | The use of apoptotic cells ex vivo to generate regulatory t cells |
| CN106659740A (en) * | 2014-08-08 | 2017-05-10 | 南加州大学阿尔弗雷德·E·曼生物医学工程研究所 | Apoptotic bodies |
| CN109221082A (en) * | 2018-09-14 | 2019-01-18 | 上海慧存医疗科技有限公司 | Cells frozen storing liquid, cryopreservation resuscitation method and its application |
| CN109329271A (en) * | 2018-11-19 | 2019-02-15 | 成都清科生物科技有限公司 | A kind of clinical mescenchymal stem cell freezen protective liquid and preparation method thereof |
| CN109566604A (en) * | 2019-01-31 | 2019-04-05 | 北京华龛生物科技有限公司 | A kind of method that three-dimensional microcarrier In-situ condensation saves cell |
| CN109619089A (en) * | 2018-12-27 | 2019-04-16 | 湖南赛诺生物科技股份有限公司 | A kind of islet cells cryopreservation solution and its application method |
| CN112167241A (en) * | 2019-07-04 | 2021-01-05 | 陕西佰傲干细胞再生医学有限公司 | Stem cell freezing medium and stem cell freezing and recovering method |
-
2019
- 2019-08-02 CN CN201910711147.4A patent/CN110367242A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101351118A (en) * | 2005-11-02 | 2009-01-21 | 特拉科斯有限公司 | The use of apoptotic cells ex vivo to generate regulatory t cells |
| CN106659740A (en) * | 2014-08-08 | 2017-05-10 | 南加州大学阿尔弗雷德·E·曼生物医学工程研究所 | Apoptotic bodies |
| CN109221082A (en) * | 2018-09-14 | 2019-01-18 | 上海慧存医疗科技有限公司 | Cells frozen storing liquid, cryopreservation resuscitation method and its application |
| CN109329271A (en) * | 2018-11-19 | 2019-02-15 | 成都清科生物科技有限公司 | A kind of clinical mescenchymal stem cell freezen protective liquid and preparation method thereof |
| CN109619089A (en) * | 2018-12-27 | 2019-04-16 | 湖南赛诺生物科技股份有限公司 | A kind of islet cells cryopreservation solution and its application method |
| CN109566604A (en) * | 2019-01-31 | 2019-04-05 | 北京华龛生物科技有限公司 | A kind of method that three-dimensional microcarrier In-situ condensation saves cell |
| CN112167241A (en) * | 2019-07-04 | 2021-01-05 | 陕西佰傲干细胞再生医学有限公司 | Stem cell freezing medium and stem cell freezing and recovering method |
Non-Patent Citations (1)
| Title |
|---|
| 陈松林 等: "《鱼类精子和胚胎低温冷冻保存理论与技术》", 30 May 2007, 中国农业出版社 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110946129A (en) * | 2019-11-08 | 2020-04-03 | 浙江卫未生物医药科技有限公司 | High-survival-rate frozen stock solution after cell recovery |
| WO2021190620A1 (en) * | 2020-03-27 | 2021-09-30 | 上海我武干细胞科技有限公司 | Cell preservation fluid and kit thereof |
| CN111587877A (en) * | 2020-06-28 | 2020-08-28 | 上海交通大学医学院附属第九人民医院 | Stem cell cryopreservation protective agent, preparation method and application |
| CN112806355A (en) * | 2021-01-20 | 2021-05-18 | 山东科硕生物技术有限公司 | Cell preservation solution and preparation method and application thereof |
| CN112715533A (en) * | 2021-02-26 | 2021-04-30 | 康妍葆(北京)干细胞科技有限公司 | Cryopreservation solution and cryopreservation method for mesenchymal stem cells |
| CN112715533B (en) * | 2021-02-26 | 2022-04-29 | 康妍葆(北京)干细胞科技有限公司 | Cryopreservation solution and cryopreservation method for mesenchymal stem cells |
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Application publication date: 20191025 |