CN110361369A - A kind of Hg based on molecular beacon aptamers2+Detection method - Google Patents
A kind of Hg based on molecular beacon aptamers2+Detection method Download PDFInfo
- Publication number
- CN110361369A CN110361369A CN201910523651.1A CN201910523651A CN110361369A CN 110361369 A CN110361369 A CN 110361369A CN 201910523651 A CN201910523651 A CN 201910523651A CN 110361369 A CN110361369 A CN 110361369A
- Authority
- CN
- China
- Prior art keywords
- molecular beacon
- detection method
- aptamers
- experimental group
- beacon aptamers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6432—Quenching
Abstract
The present invention provides a kind of Hg based on molecular beacon aptamers2+Detection method is related to Hg2+Detection technique field.The Hg2+Detection method mainly includes the setting up, detect of the design of molecular beacon aptamers, the preparation of buffer system, control group and experimental group, establishes standard curve, quantitative analysis.The present invention overcomes the deficiencies in the prior art, using the T base and Hg in molecular beacon aptamers2+Form T-Hg2+The structure of-T, aptamer and Hg2+Specific binding caused the conformational change of aptamer, to widen the distance between fluorophor and quencher, therefore the principle that fluorescence intensity increases, promote the sensitivity of detection, facilitate testing staff to observe detection, and each solution allocation is easy in detection process, is easy to use, manpower and material resources are reduced, are suitable for promoting the use of.
Description
Technical field
The present invention relates to Hg2+A kind of detection technique field, and in particular to Hg based on molecular beacon aptamers2+Detection side
Method.
Background technique
Hg2+It is the ionic forms of mercury, in modern industry, the compound of mercury is all indispensable in many aspects.Closely
Nian Lai causes the event of mercury poisoning countless since people are in the rapid development of industrial aspect.Hg2+Due to strong toxicity, to body
Body damage is big, therefore develops quickly and easily Hg2+Detection method just seem especially urgent.Hg at present2+Detection method have very
It is a variety of, it mainly include that atomic absorption spectrum, atomic emission spectrum, inductively coupled plasma mass spectrometry and electrochemistry etc. are a variety of
Method, but these methods need operation and the large size detection machine of professional person, and there is relatively complicated detection steps
Suddenly, Hg is leveraged2+The timeliness of detection.
Aptamer is the segment that oligonucleotide is filtered out by in-vitro screening technology (SELEX), is had very strong
The affinity of recognition capability and height.It experienced the development course of more than ten years, aptamer is in sensor, molecular biosciences
Etc. various fields are widely applied.Molecular beacon (Molecular beacon) be it is a kind of in the end 5' and 3' from figure
At the stem ring double labelling oligonucleotide probe of a hairpin structure, the nucleic acid array complementation at both ends is matched, therefore marks at one end
Fluorophor be close to label in the quenching group of the other end, Photo-induced electron transfer, which occurs, leads to fluorophor fluorescence
It is quenched, molecular beacon has the characteristics that many outstanding simultaneously, and the value of background detection is low, and sensitivity response is high, specific recognition
It is high, easy to operate, need not be with the probe separates reacted not yet in real-time monitoring.In recent years, molecular beacon was successful
In multiple fields application.
Summary of the invention
In view of the shortcomings of the prior art, the present invention provides a kind of Hg based on molecular beacon aptamers2+Detection method improves
Hg2+The sensitivity of detection, facilitates testing staff to detect, while saving human and material resources is suitable for promoting the use of.
In order to achieve the above object, technical solution of the present invention is achieved by the following technical programs:
A kind of Hg based on molecular beacon aptamers2+Detection method, the Hg2+Detection method includes the following steps:
(1) design of molecular beacon aptamers: design is by the ssDNA probe of 29 base compositions, and wherein DNA probe contains
5 ' ends stem ring the hairpin structure mutually close with 3 ' ends, and 5 ' end mark FAM, 3 ' end mark DABCYL；
(2) preparation of buffer system: preparation pH is 8.1, Na+Concentration is 40mmol/L, Mg2+Concentration is the slow of 10mmol/L
System is rushed, it is spare；
(3) control group and experimental group are set up: certain density above-mentioned molecular beacon aptamers are added into buffer system,
It adds to be detected containing Hg2+Solution, after mixing formed experimental group it is spare, into buffer system be added with experimental group comparable sodium and
In contrast the molecular beacon aptamers of volume are organized spare after mixing；
(4) it detects: will be incubated under above-mentioned control group and experimental group equal conditions, it is rear to detect fluorescence intensity in each group,
Calculate the variation of fluorescence intensity；
(5) standard curve is established, determines minimum detectability and the range of linearity；
(6) quantitative analysis is carried out to experimental group according to standard curve.
Preferably, ssDNA probe from 5 ' ends to 3 ' sequence is in the step (1)
GAACACCCCCTTCTTCTTCCTTGTTGTTC。
Preferably, the concentration of the molecular beacon aptamers is 0.08 μm of ol/L.
Preferably, the buffer system added in step (3) experimental group, molecular beacon aptamers and to be detected containing Hg2+
Buffer system in solution and control group, molecular beacon aptamers additive amount be addition peer accumulated amount.
Preferably, the control group and the incubation time of experimental group are 10min.
Preferably, the detection method in the step (4) is to take control group respectively and experimental group volume is the detection of 100 μ L
Sample liquid is added 96 orifice plate of black, carries out on multi-functional plate reading machine, and excitation wavelength is 485nm, launch wavelength 535nm.
Preferably, established in the step (5) standard curve mode be using the changing value of fluorescence intensity as ordinate,
Hg2+Concentration be abscissa draw standard curve.
The present invention provides a kind of Hg based on molecular beacon aptamers2+Detection method, advantage is compared with prior art:
The present invention is that fluorophor and quencher are connected to aptamers using the basic principle of molecular beacon aptamers
On, and since the molecular beacon aptamers of design are hairpin structures, when fluorophor and quencher are close together, due to
The effect of fluorescence resonance energy transfer, the fluorescence signal being detected by this time is lower, but after target molecules are added, molecular beacon
The secondary con of aptamers is changed, and fluorescence intensity substantially increases.In the present invention, it devises for Hg2+Molecule letter
Aptamers are marked, Hg is worked as2+T base and Hg after addition, in molecular beacon aptamers2+Form T-Hg2+The structure of-T, nucleic acid are suitable
Ligand and Hg2+Specific binding caused the conformational change of aptamer, thus widen fluorophor and quencher it
Between distance, therefore fluorescence intensity increases, and promotes the sensitivity of detection, and testing staff is facilitated to observe detection, and detection process
In each solution allocation it is easy, be easy to use, reduce manpower and material resources, be suitable for promoting the use of.
Detailed description of the invention
Fig. 1: the secondary structure schematic diagram of molecular beacon aptamers；
Fig. 2: molecular beacon aptamers detection schematic diagram；
Fig. 3: Hg2+Examination criteria curve graph；
Fig. 4: influence histogram of the different metal ions to molecular beacon fluorescence intensity.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below with reference to the embodiment of the present invention pair
Technical solution in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is a part of the invention
Embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making wound
Every other embodiment obtained under the premise of the property made labour, shall fall within the protection scope of the present invention.
Embodiment 1:
A kind of Hg based on molecular beacon aptamers2+Detection method, the Hg2+Detection method includes the following steps:
(1) design of molecular beacon aptamers: design is by the ssDNA probe of 29 base compositions, and wherein DNA probe contains
5 ' ends stem ring the hairpin structure mutually close with 3 ' ends, and 5 ' end mark FAM, 3 ' end mark DABCYL；
(2) preparation of buffer system: preparation pH is 8.1, Na+Concentration is 40mmol/L, Mg2+Concentration is the slow of 10mmol/L
System is rushed, it is spare；
(3) control group and experimental group are set up: certain density above-mentioned molecular beacon aptamers are added into buffer system,
It adds to be detected containing Hg2+Solution, after mixing formed experimental group it is spare, into buffer system be added with experimental group comparable sodium and
In contrast the molecular beacon aptamers of volume are organized spare after mixing；
(4) it detects: will be incubated under above-mentioned control group and experimental group equal conditions, it is rear to detect fluorescence intensity in each group,
Calculate the variation of fluorescence intensity；
(5) standard curve is established, determines minimum detectability and the range of linearity；
(6) quantitative analysis is carried out to experimental group according to standard curve.
Wherein, ssDNA probe from 5 ' ends to 3 ' sequence is in the step (1)
GAACACCCCCTTCTTCTTCCTTGTTGTTC；The concentration of the molecular beacon aptamers is 0.08 μm of ol/L；, the step
(3) buffer system added in experimental group, molecular beacon aptamers and to be detected containing Hg2+Buffer system in solution and control group,
The additive amount of molecular beacon aptamers is 50 μ L；The control group and the incubation time of experimental group are 10min；The step
(4) detection method in is to take control group respectively and experimental group volume is the detection sample liquid of 100 μ L, and 96 orifice plate of black is added,
It is carried out on multi-functional plate reading machine, and excitation wavelength is 485nm, launch wavelength 535nm；Standard song is established in the step (5)
The mode of line is the Hg using the changing value of fluorescence intensity as ordinate2+Concentration be abscissa draw standard curve.
With this condition, Hg2+The standard curve y=3506.6x-960.57 of detection, linear regression coeffficient R2=
0.9901, the range of linearity is 0.4~10 μm of ol/L, and detection is limited to 0.2254 μm of ol/L, precision RSD 4.9%.In water sample
Hg2+Recovery of standard addition be 95.00%~99.25% between.(as shown in Figure 3)
Embodiment 2:
Measure the method for the present invention Hg2+The rate of recovery:
(1) Hg is chosen2+Content is that the water sample of 0.4 μm of ol/L is test water sample, and test water sample is divided into three groups, every group of 1L,
The Hg of 8 μm of ol, 4 μm of ol and 0.8 μm of ol are added respectively2+, spare after mixing；
(2) above-mentioned each group water sample is detected into its Hg using the detection method of embodiment 1 respectively2+Measured value, calculate back
Yield, as a result as shown in the table:
Embodiment 3:
Using detection method described in embodiment 1, replace respectively to be detected containing Hg2+Solution is containing Zn2+Solution contains Al3+It is molten
Liquid contains Mn2+Solution contains Sn2+Solution contains Co2+Solution contains K+Solution contains Cu2+Solution contains Ba2+Solution contains Ca2+Solution and contain
Cr2+Solution measures influence of the different metal ions to molecular beacon fluorescence intensity, as a result as shown in Figure 4.
Detection method can effectively detect Hg solution as can be seen from the above embodiments2+Content, and other metals
Ion pair detection influences lower, Detection accuracy height, suitable to promote the use of.
It should be noted that, in this document, relational terms such as first and second and the like are used merely to a reality
Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation
In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to
Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those
Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or equipment
Intrinsic element.In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that
There is also other identical elements in process, method, article or equipment including the element.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although with reference to the foregoing embodiments
Invention is explained in detail, those skilled in the art should understand that: it still can be to aforementioned each implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features；And these modification or
Replacement, the spirit and scope for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution.
Claims (7)
1. a kind of Hg based on molecular beacon aptamers2+Detection method, which is characterized in that the Hg2+Detection method includes following
Step:
(1) design of molecular beacon aptamers: design is by the ssDNA probe of 29 base compositions, and wherein DNA probe is containing 5 ' ends
Hold the stem ring hairpin structure mutually close with 3 ' ends, and 5 ' end mark FAM, 3 ' end mark DABCYL；
(2) preparation of buffer system: preparation pH is 8.1, Na+Concentration is 40mmol/L, Mg2+Concentration is the buffer body of 10mmol/L
System, it is spare；
(3) control group and experimental group are set up: it is added certain density above-mentioned molecular beacon aptamers into buffer system, then plus
Enter to be detected containing Hg2+Solution, formation experimental group is spare after mixing, is added and experimental group comparable sodium and volume into buffer system
Molecular beacon aptamers, in contrast organized after mixing spare；
(4) it detects: will be incubated under above-mentioned control group and experimental group equal conditions, it is rear to detect fluorescence intensity in each group, it calculates
The variation of fluorescence intensity；
(5) standard curve is established, determines minimum detectability and the range of linearity；
(6) quantitative analysis is carried out to experimental group according to standard curve.
2. a kind of Hg based on molecular beacon aptamers according to claim 12+Detection method, it is characterised in that: described
It is GAACACCCCCTTCTTCTTCCTTGTTGTTC that ssDNA probe, which holds the sequence to 3 ' from 5 ', in step (1).
3. a kind of Hg based on molecular beacon aptamers according to claim 12+Detection method, it is characterised in that: described
The concentration of molecular beacon aptamers is 0.08 μm of ol/L.
4. a kind of Hg based on molecular beacon aptamers according to claim 12+Detection method, it is characterised in that: described
The buffer system added in step (3) experimental group, molecular beacon aptamers and to be detected containing Hg2+Buffer body in solution and control group
It is, the additive amount of molecular beacon aptamers is addition peer accumulated amount.
5. a kind of Hg based on molecular beacon aptamers according to claim 12+Detection method, it is characterised in that: described
Control group and the incubation time of experimental group are 10min.
6. a kind of Hg based on molecular beacon aptamers according to claim 12+Detection method, it is characterised in that: described
Detection method in step (4) is to take control group respectively and experimental group volume is the detection sample liquid of 100 μ L, and 96 hole of black is added
Plate carries out on multi-functional plate reading machine, and excitation wavelength is 485nm, launch wavelength 535nm.
7. a kind of Hg based on molecular beacon aptamers according to claim 12+Detection method, it is characterised in that: described
The mode that standard curve is established in step (5) is the Hg using the changing value of fluorescence intensity as ordinate2+Concentration be abscissa draw
Standard curve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910523651.1A CN110361369A (en) | 2019-06-17 | 2019-06-17 | A kind of Hg based on molecular beacon aptamers2+Detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910523651.1A CN110361369A (en) | 2019-06-17 | 2019-06-17 | A kind of Hg based on molecular beacon aptamers2+Detection method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110361369A true CN110361369A (en) | 2019-10-22 |
Family
ID=68217352
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910523651.1A Pending CN110361369A (en) | 2019-06-17 | 2019-06-17 | A kind of Hg based on molecular beacon aptamers2+Detection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110361369A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111856010A (en) * | 2020-07-17 | 2020-10-30 | 中国石油大学(华东) | Molecular beacon, and construction method and application thereof |
| CN112285078A (en) * | 2020-10-14 | 2021-01-29 | 安庆师范大学 | Novel mercury ion on-site detection method based on intelligent DNA hydrogel |
| CN113624724A (en) * | 2020-05-07 | 2021-11-09 | 廖世奇 | Multi-element detection and analysis method of aptamer molecular beacon for target molecule |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000070329A1 (en) * | 1999-05-14 | 2000-11-23 | Brandeis University | Nucleic acid-based detection |
| US20100105039A1 (en) * | 2008-06-03 | 2010-04-29 | Yi Lu | Label-free colorimetric detection |
| WO2011035135A1 (en) * | 2009-09-18 | 2011-03-24 | The Trustees Of The University Of Pennsylvania | Novel molecular beacons |
| CN104212803A (en) * | 2014-08-29 | 2014-12-17 | 河南省农业科学院 | Nucleic acid aptamer sequence and detection method for quantitative and rapid detection of mercury ions |
| CN104894137A (en) * | 2015-06-02 | 2015-09-09 | 安徽科技学院 | Malathion aptamer, derivative and application thereof |
| CN105510287A (en) * | 2015-11-30 | 2016-04-20 | 环境保护部华南环境科学研究所 | Signal-amplified high-sensitivity detection method for Hg<2+> in water environments |
| CN105579591A (en) * | 2013-12-13 | 2016-05-11 | 豪夫迈·罗氏有限公司 | Detecting single nucleotide polymorphism using hydrolysis probes with 3' hairpin structure |
| CN106546724A (en) * | 2015-09-23 | 2017-03-29 | 中国医学科学院药用植物研究所 | A kind of new method of molecular beacon probe quick detection ochratoxin A |
| CN107436297A (en) * | 2017-07-19 | 2017-12-05 | 太原理工大学 | Mercury ion detecting method in water based on hairpin-type DNA probe |
| CN107727623A (en) * | 2017-10-12 | 2018-02-23 | 广东省生态环境技术研究所 | A kind of mercury ion fluorescent detection kit |
-
2019
- 2019-06-17 CN CN201910523651.1A patent/CN110361369A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000070329A1 (en) * | 1999-05-14 | 2000-11-23 | Brandeis University | Nucleic acid-based detection |
| US20100105039A1 (en) * | 2008-06-03 | 2010-04-29 | Yi Lu | Label-free colorimetric detection |
| WO2011035135A1 (en) * | 2009-09-18 | 2011-03-24 | The Trustees Of The University Of Pennsylvania | Novel molecular beacons |
| CN105579591A (en) * | 2013-12-13 | 2016-05-11 | 豪夫迈·罗氏有限公司 | Detecting single nucleotide polymorphism using hydrolysis probes with 3' hairpin structure |
| CN104212803A (en) * | 2014-08-29 | 2014-12-17 | 河南省农业科学院 | Nucleic acid aptamer sequence and detection method for quantitative and rapid detection of mercury ions |
| CN104894137A (en) * | 2015-06-02 | 2015-09-09 | 安徽科技学院 | Malathion aptamer, derivative and application thereof |
| CN106546724A (en) * | 2015-09-23 | 2017-03-29 | 中国医学科学院药用植物研究所 | A kind of new method of molecular beacon probe quick detection ochratoxin A |
| CN105510287A (en) * | 2015-11-30 | 2016-04-20 | 环境保护部华南环境科学研究所 | Signal-amplified high-sensitivity detection method for Hg<2+> in water environments |
| CN107436297A (en) * | 2017-07-19 | 2017-12-05 | 太原理工大学 | Mercury ion detecting method in water based on hairpin-type DNA probe |
| CN107727623A (en) * | 2017-10-12 | 2018-02-23 | 广东省生态环境技术研究所 | A kind of mercury ion fluorescent detection kit |
Non-Patent Citations (2)
| Title |
|---|
| AKIRA ONO AND HUMIKA TOGASHI: ""Highly Selective Oligonucleotide-Based Sensor for Mercury(ii) in Aqueous Solutions"", 《ANGEW.CHEM》 * |
| 邹克琴: "《基因工程原理和技术》", 31 January 2009, 浙江大学出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113624724A (en) * | 2020-05-07 | 2021-11-09 | 廖世奇 | Multi-element detection and analysis method of aptamer molecular beacon for target molecule |
| CN111856010A (en) * | 2020-07-17 | 2020-10-30 | 中国石油大学(华东) | Molecular beacon, and construction method and application thereof |
| CN111856010B (en) * | 2020-07-17 | 2023-09-22 | 中国石油大学(华东) | Molecular beacon, construction method and application thereof |
| CN112285078A (en) * | 2020-10-14 | 2021-01-29 | 安庆师范大学 | Novel mercury ion on-site detection method based on intelligent DNA hydrogel |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110361369A (en) | A kind of Hg based on molecular beacon aptamers2+Detection method | |
| Wolf et al. | Recommended soil tests for macro and micronutrients | |
| CN105699355A (en) | SERS (Surface Enhanced Raman Scattering) sensor for detecting mercury ions as well as preparation method and detection method thereof | |
| CN102252987B (en) | Method for detecting protein content | |
| CN103411942A (en) | Fluorescent probe method for detecting Cu<2+> and cell imaging | |
| CN104212803A (en) | Nucleic acid aptamer sequence and detection method for quantitative and rapid detection of mercury ions | |
| WO2021093530A1 (en) | Lead ion nucleic acid aptamer, electrochemical sensor and preparation method | |
| CN106442515A (en) | Simple and low-cost silver ion visual quantitative detection method | |
| CN101586145A (en) | Analyzing method for detecting activity of soil xylanase | |
| CN109211877A (en) | Plasma spectrum analysis method | |
| CN101586146B (en) | Analyzing method for detecting activity of soil xylanase | |
| CN107144565B (en) | Method for measuring chitosan concentration in aqueous solution | |
| CN105954265A (en) | Determining method for trace nickel in high-salinity wastewater | |
| CN102621120A (en) | Method for detecting mercury ion residue of fluorescent signal conversion mechanism based on nucleic acid aptamer structure | |
| CN102242181B (en) | Flue gas condensate cytotoxicity determination method based on cell electronic sensor | |
| LU503082B1 (en) | An Hg2+ detection method based on molecular beacon aptamers | |
| CN113402470B (en) | Multichannel reversible colorimetric mercury ion fluorescent probe, preparation method and application | |
| CN110655919B (en) | Copper ion fluorescent probe and preparation method and application thereof | |
| CN107389569A (en) | BOD detection method in a kind of water | |
| CN101532984B (en) | Preparation method of ion selective electrode capable of measuring trace chromium (III) | |
| CN110412024B (en) | Hg2+ detection method based on aptamer and nanogold | |
| CN106053410B (en) | The compositional liquor and its application of Quercetin and cyclodextrin | |
| CN111650167A (en) | Nanocluster beacon type multifunctional fluorescence sensor containing splitting aptamer | |
| CN110877902A (en) | Preparation method and application of flexible and high-selectivity non-enzymatic uric acid electrode carbon fiber membrane of self-supporting carbon fiber | |
| CN105784665B (en) | The compositional liquor and its application of Isorhamnetin and cyclodextrin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191022 |