CN110361238A - A kind of device and its application for cutting and dyeing - Google Patents
A kind of device and its application for cutting and dyeing Download PDFInfo
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- CN110361238A CN110361238A CN201810311236.5A CN201810311236A CN110361238A CN 110361238 A CN110361238 A CN 110361238A CN 201810311236 A CN201810311236 A CN 201810311236A CN 110361238 A CN110361238 A CN 110361238A
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- 238000004043 dyeing Methods 0.000 title claims abstract description 72
- 238000005520 cutting process Methods 0.000 title abstract description 29
- 239000007787 solid Substances 0.000 claims abstract description 51
- 238000003384 imaging method Methods 0.000 claims abstract description 24
- 235000013311 vegetables Nutrition 0.000 claims abstract description 8
- 239000003086 colorant Substances 0.000 claims description 29
- 239000007788 liquid Substances 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 17
- 239000002699 waste material Substances 0.000 claims description 17
- 239000000463 material Substances 0.000 claims description 8
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims description 3
- 239000013078 crystal Substances 0.000 claims description 3
- 229910052740 iodine Inorganic materials 0.000 claims description 3
- 239000011630 iodine Substances 0.000 claims description 3
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 claims description 2
- 239000000975 dye Substances 0.000 claims 3
- 239000010919 dye waste Substances 0.000 claims 1
- 235000007164 Oryza sativa Nutrition 0.000 abstract description 43
- 235000009566 rice Nutrition 0.000 abstract description 43
- 238000012545 processing Methods 0.000 abstract description 5
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 110
- 241000209094 Oryza Species 0.000 description 42
- 235000013339 cereals Nutrition 0.000 description 10
- 238000011160 research Methods 0.000 description 6
- 241000209140 Triticum Species 0.000 description 4
- 235000021307 Triticum Nutrition 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000011084 recovery Methods 0.000 description 4
- 229920002472 Starch Polymers 0.000 description 3
- 240000005979 Hordeum vulgare Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- 235000007199 Panicum miliaceum Nutrition 0.000 description 2
- 241000209056 Secale Species 0.000 description 2
- 235000007238 Secale cereale Nutrition 0.000 description 2
- 240000006394 Sorghum bicolor Species 0.000 description 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 2
- 244000022185 broomcorn panic Species 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 235000019890 Amylum Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 108010050181 aleurone Proteins 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 235000021329 brown rice Nutrition 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- IQFVPQOLBLOTPF-HKXUKFGYSA-L congo red Chemical compound [Na+].[Na+].C1=CC=CC2=C(N)C(/N=N/C3=CC=C(C=C3)C3=CC=C(C=C3)/N=N/C3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)N)=CC(S([O-])(=O)=O)=C21 IQFVPQOLBLOTPF-HKXUKFGYSA-L 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 229950003937 tolonium Drugs 0.000 description 1
- HNONEKILPDHFOL-UHFFFAOYSA-M tolonium chloride Chemical compound [Cl-].C1=C(C)C(N)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 HNONEKILPDHFOL-UHFFFAOYSA-M 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2873—Cutting or cleaving
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
- G01N2001/364—Embedding or analogous mounting of samples using resins, epoxy
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to a kind of device for cutting and dyeing and its applications.Described device includes fixed device, cutter device, dyeing apparatus and control device, preferably further includes imaging device.Described device can cut solid sample to be measured, especially vegetable seeds sample or its processing product (such as rice), and acquisition section is smooth, organizes clearly cleaved products.Dyeing apparatus of the invention makes it expose interior tissue, it can be achieved that high-throughput operate, and is integrated with the function of dyeing and/or imaging especially suitable for cutting to vegetable seeds or its processing product.
Description
Technical field
The present invention relates to biological sample manufacture fields；More particularly it relates to a kind of dress for cutting and dyeing
It sets and its applies.
Background technique
There are structures abundant for the seed of some crops gramineous.For example, cereal crops gramineous, are primarily present
The structures such as grain husk, kind skin, aleurone, plumule, endosperm.Facilitate the property of understanding seed for research, the observation of crop seed structure
Shape variation, quality etc. are that botany studies indispensable one aspect.
However, in the prior art, not meeting still and being cut, contaminated needed for crop seed research, for crop seed
Color, observation dedicated unit.Currently, researcher in order to obtain crop seed section, often takes the mode manually cut, no
It is convenient, poor accuracy, and can not achieve high-throughput, large-scale research.
Therefore, this field needs, which are developed, meets the required new tool of crop seed research, conveniently, is accurately planted with providing
The cutting of species, realizes high pass quantity research.
Summary of the invention
The purpose of the present invention is to provide a kind of device for cutting and dyeing and its applications.
In the first aspect of the present invention, a kind of device for solid sample to be measured to be cut and dyed is provided, is wrapped
It includes:
Fixed device, is used to fix the sample panel of solid sample to be measured；
Cutter device, for being cut to solid sample to be measured comprising removable and rotation cutter；
Dyeing apparatus, comprising at least one coloring agent, for being dyed to the sample in sample panel；It includes at least one
Pipeline is connected to fixed device；
Control device controls the operation of fixed device, cutter device, dyeing apparatus.
In a preferred embodiment, include in the fixation device: clamping device, for fixing the sample in sample panel.
In another preferred example, the fixation device can carry out the movement (such as left and right translation) of horizontal direction and/or hang down
Histogram to movement (such as moving up and down).
In another preferred example, the clamping device is set as small as possible with the contact area of solid sample to be measured.
In another preferred example, the cutter is thin type cutter.
In another preferred example, the sample panel is to be uniformly distributed grilled plate.
In another preferred example, the sample panel is evenly distributed with the grid of M × N, the positive integer that wherein M is 1~50
(for example 4,5,10,15,20,30,40)；The positive integer (for example 4,5,10,15,20,30,40) that N is 1~50.
In another preferred example, the grid quantity of the sample panel for example comprising: 5,10,12,24,25,36,48,49,
72,96,100,150,192,200,300,384 holes.
In another preferred example, the sample panel is porous plate, has bottom.
In another preferred example, the fixation device includes at least one container for being used to place sample panel, from dyeing
The coloring agent of device output can be input in the container, to dye to the cleaved products in sample panel.
In another preferred example, the container further includes a pipeline, for dyeing waste liquid to be discharged.
In another preferred example, in the pipeline, further includes: pump, for driving the outflow of dyeing waste liquid.
In another preferred example, in the pipeline that dyeing apparatus is connected with fixed device, further includes: pump, for driving dye
The fixed device of the input of toner.
In another preferred example, the solid sample to be measured includes: vegetable seeds or it processes product, tissue sample.
In another preferred example, the vegetable seeds is crop seed.Preferably, the fixation device is to be suitble to admittedly
The construction of ordered goods seed.
In another preferred example, the crop includes: gramineous crop, and the gramineous crop includes but unlimited
In: rice, wheat, barley, rye, sorghum, corn, broomcorn millet.
In another preferred example, the device further include: imaging device is used to shoot cleaved products, can be by institute
The control device control stated.
In another preferred example, the imaging device is photomicrographic device.
In another preferred example, the imaging device includes camera lens, controls focal length by the control device.
In another aspect of this invention, the application of the device is provided, for solid sample to be measured carry out cutting and
Dyeing.
In another aspect of this invention, it provides and a kind of solid sample to be measured is cut and dyed using the device
Method, which comprises
(1) solid sample to be measured is coated with embedded material, obtains the sample being coated；
(2) sample being coated that step (1) obtains is placed in sample panel, sample panel is fixed on fixed device；
(3) sample being coated is cut with cutter device, obtains cleaved products；
(4) coloring agent in dyeing apparatus is input to fixed device, the cleaved products in sample panel is dyed, are obtained
Obtain the cleaved products being colored on section.
In a preferred embodiment, the method also includes: with imaging device shooting the cleaved products through dyeing section.
In another preferred example, the coloring agent includes but is not limited to: iodine solution or sarranine-crystal violet dye liquor are examined
Mas bright blue R250 dye liquor.
Other aspects of the invention are apparent to those skilled in the art due to this disclosure
's.
Detailed description of the invention
The schematic top plan view that section components arrange in Fig. 1, a kind of cutting of specific embodiment of the invention and dyeing apparatus.
Fig. 2, a kind of cutting of specific embodiment (embodiment 1) of the invention and dyeing apparatus schematic diagram.
Fig. 3, a kind of cutting of specific embodiment (embodiment 2) of the invention and dyeing apparatus schematic diagram.
Pictorial diagram after Fig. 4, the dyeing of rice section.
It is each that the reference numerals are as follows:
1: clamping device；
2: for placing the grid (hole) of sample；
3: sample panel；
4: cutter device；41: blade
5: dyeing apparatus；
6: control device；
7: imaging device；71: camera lens；
8: pump；
9: fixed device；
10: waste liquid recovery apparatus.
Specific embodiment
The present inventor passes through in-depth study, develops a kind of for cutting and dyeing apparatus, including fixed device, cutting
Device, dyeing apparatus and control device.The device is suitable for solid sample to be measured, and especially vegetable seeds sample is cut
It cuts, acquisition section is smooth, organizes clearly cleaved products.
Dyeing apparatus of the invention makes it expose interior tissue, and integrate particularly suitable for cutting to vegetable seeds
The function of dyeing and/or imaging.
Fixed device
The fixation device is for fixing sample panel, and sample panel is for placing solid sample to be measured.The sample panel
For porous plate, bottom is had.
In a preferred embodiment of the present invention, include in the fixation device: clamping device, for fixing in sample panel
Sample.The clamping device can be one or more, such as can be 1~100, such as 5,10,15,20,30,50,60,
80, so as to multiple samples are clamped side by side in an operational process and subsequent scale operation.Preferably
Ground, the clamping device is set as small as possible with the contact area of solid sample to be measured, as long as can guarantee for be measured
Solid sample has good fixation, does not slide.
In a preferred embodiment of the present invention, the clamping device presss from both sides solid sample to be measured along the edge of sample panel
Tightly.
In a preferred embodiment of the present invention, the fixation device can carry out the movement of horizontal direction (such as left and right translation)
And/or the movement (such as moving up and down) of vertical direction, so that the sample panel fixed on fixed device can be transported to
On appropriate position.
In a preferred embodiment of the present invention, the fixation device includes at least one container, and the sample panel is consolidated
It can be input into the container by pipeline due to the coloring agent in the container of the fixed device, exported from dyeing apparatus, with
Cleaved products in sample panel are dyed.
In a preferred embodiment of the present invention, the container further includes another pipeline, for dyeing waste liquid to be discharged.Preferably
Waste liquid recovery apparatus is arranged to recycle the waste liquid in ground.
From the container further include: pump, for driving the outflow of dyeing waste liquid；Preferably flow out to waste liquid recovery apparatus.
In a preferred embodiment of the present invention, the sample panel is the grid for being evenly distributed with M × N, and wherein M is 1~50
Positive integer, for example 4,5,10,15,20,30,40；The positive integer that N is 1~50, for example 4,5,10,15,20,30,40.For example,
The grid quantity of the sample panel for example comprising: 5,10,12,24,25,36,48,49,72,96,100,150,192,200,
300,384 holes.
In a preferred embodiment of the present invention, the sample panel is high-throughput porous plate, this is commercialized product, packet
It includes but is not limited to: 16 orifice plates, 24 orifice plates, 48 orifice plates, 96 orifice plates, 192 orifice plates, etc..
Cutter device
The cutter device is for cutting solid sample to be measured comprising removable and rotation cutter.
In a preferred embodiment of the present invention, cutter can be driven with device to be cut or control device, be rotated or upper
The movement of movement and run-off the straight direction on lower left and right directions, to when needed, can be carried out to solid sample to be measured
The cutting operation of different angle.
In a preferred embodiment of the present invention, the cutter is thin type cutter.For example, machine-cut is with a thickness of 2 microns.
Dyeing apparatus
For the dyeing apparatus for dyeing to the sample in sample panel, interior includes at least one coloring agent,
It is connected to including at least one pipeline with fixed device.It may include multiple containers in dyeing apparatus, each container is packed into a kind of dye
Toner, the coloring agent in each container can be identical or different.If each container is assigned at least there are when multiple containers
One pipeline reaches fixed device to convey the coloring agent.
In a preferred embodiment of the present invention, the coloring agent of dyeing apparatus output is transported to putting for fixed device by pipeline
It sets in the container of sample panel.
Dyeing apparatus is output in the pipeline of fixed device, further includes: pump, for driving coloring agent to be input to fixed device
In.
Imaging device
Device of the present invention further include: imaging device is used to shoot cleaved products.The imaging device includes
Camera lens controls focal length by the control device.
In a preferred embodiment of the present invention, the imaging device is photomicrographic device.
Control device
The control device controls the operation of fixed device, cutter device, dyeing apparatus.
In a preferred embodiment of the present invention, the control device is computer controller, passes through setting for specific program
It sets, plays: driving the movement of fixed device, it is fixed, it clamps, unclamp etc.；The movement of driving cutter device, the movement of cutter,
Rotation and cutting etc.；Drive the input or output etc. of coloring agent in dyeing apparatus；The switch etc. pumped in driving pipeline；And/or control
It is formed as the movement, rotation, focusing etc. of device.
The solid sample can repeatedly be cut (as cut along longitudinal direction), obtain the section of different location, finally can be with
Three-dimensional data are obtained with computer disposal, such as distributed in three dimensions of the starch granules in endosperm.
The running of device
The present invention also provides the method that solid sample to be measured is cut and dyed using the device, the sides
Method includes:
(1) solid sample to be measured is coated with embedded material, obtains the sample being coated；
(2) sample being coated that step (1) obtains is placed in sample panel, sample panel is fixed on fixed device；
(3) sample being coated is cut with cutter device, obtains cleaved products；
(4) coloring agent in dyeing apparatus is input to fixed device, the cleaved products in sample panel is dyed, are obtained
Obtain the cleaved products that section is dyed.
Unless otherwise stated, sample to be tested of the present invention is solid sample.The solid sample to be measured includes: to plant
Species or its processing product, tissue sample.The device of the invention, especially suitable for being cut to crop seed.Described
Crop includes: gramineous crop, and the gramineous crop includes but is not limited to: rice, wheat, barley, rye, sorghum, beautiful
Rice, broomcorn millet.Preferably, the crop is rice, wheat.
The processing product of the vegetable seeds be, for example, it is some processed on the basis of the seed naturally obtained after
Product, such as rice, wheat by decladding (glume) processing are more specific such as fructification, brown rice, rice germ, rice.By
After cutting, its endosperm structure can be showed.The device of the invention can obtain that section is smooth, clearly rice is cut tissue,
To provide accurate technical support for the amylum body quality research of rice.
In a preferred embodiment of the present invention, the method for coating solid sample to be measured includes: in the sample panel of fixed device
Solid sample to be measured is added, is slowly injected into embedded material.
In a preferred embodiment of the present invention, in cutting process, after moving cutting device to target area, cutting is adjusted
The position of knife and clamping device (accompanying sample) makes it slightly above wrap clamping device with sample, implements cutting.
The cutter device can cut rice grain in the appropriate location of solid sample to be measured, completely cut rice, shape
At entire section.For example, the solid sample to be measured be rice, in operation, after the completion of knife, feed make the knife edge with
Sample is close, and the mechanical arm where the mobile knife edge moves horizontally it, cuts the knife edge just from the cross section of rice middle and upper part.
In a preferred embodiment of the present invention, the method also includes: with imaging device shoot the cleaved products through dyeing.
The coloring agent is not particularly limited, and can be a variety of coloring agents commonly used in the art in dyeing.For example, institute
The coloring agent stated includes but is not limited to: iodine solution or sarranine-crystal violet dye liquor, coomassie brilliant blue R250 dye liquor, congo red staining
Agent, violet stain agent.
It is observed under imaging device, can get the moderate image of high resolution, contrast.Image after dyeing can be used directly
It is observed under microscope or current potential.
The major advantage of the device of the invention is:
1, high-throughput, it is multiple to handle observation simultaneously, and such as M × N number of sample (such as endosperm), this is for quality, thin
The fields such as microstructure study are meaningful, can carry out statistical analysis.
2, dyeing observation using section replaces previous slice to dye observation, reduces operation difficulty.
3, the sample embedded can repeatedly be cut (as cut along longitudinal direction), obtained the section of different location, can finally be used
Computer disposal obtains three-dimensional data, such as distributed in three dimensions of the starch granules in endosperm.
4, whole flow process can automate progress.
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.
Embodiment 1, cutting and the device one dyed
Such as FIG. 1 to FIG. 2.The device one of the cutting and dyeing includes:
Fixed device 9, is used to fix the sample panel 3 of solid sample to be measured；Include in fixed device 9: clamping device 1,
For fixing the sample in sample panel；Fixed device 9 can carry out the movement of the movement of horizontal direction；The sample panel is uniform
The plate of grid (hole shape) 2 is distributed with, includes 11 × 4 grid.Fixed device 9 further includes one for placing the appearance of sample panel
Device, the coloring agent exported from dyeing apparatus can be input in the container, to dye to the cleaved products in sample panel；Institute
The container stated further includes a pipeline, for dyeing waste liquid to be discharged；The dyeing waste liquid is discharged in waste liquid recovery apparatus 10；
It further include pump 8 in the pipeline, for driving the outflow of dyeing waste liquid.
Cutter device 4, for being cut to solid sample to be measured comprising removable and rotation cutter 41；
Dyeing apparatus 5, for being dyed to the sample in sample panel, interior coloring agent comprising pipeline and solid
Determine the connection of device 9；In the pipeline that dyeing apparatus is connected with fixed device, further includes: pump 8, for driving the input of coloring agent
Fixed device；
Control device 6 controls the operation of fixed device 9, cutter device 4, dyeing apparatus 5.
Embodiment 2, carry imaging device cutting and dyeing device two
Such as Fig. 1 and Fig. 3.The device two of the cutting and dyeing includes:
Fixed device 9, is used to fix the sample panel 3 of solid sample to be measured；Include in fixed device 9: clamping device 1,
For fixing the sample in sample panel；Fixed device 9 can carry out the movement of horizontal direction and the movement of vertical direction；Described
Sample panel is the plate for being evenly distributed with grid (hole shape) 2, includes 11 × 4 grid.Fixed device 9 further includes one for putting
The container for setting sample panel, the coloring agent exported from dyeing apparatus can be input in the container, to produce to the cutting in sample panel
Object is dyed；The container further includes a pipeline, for dyeing waste liquid to be discharged；The dyeing waste liquid is discharged to waste liquid
In recyclable device 10；It further include pump 8 in the pipeline, for driving the outflow of dyeing waste liquid.
Cutter device 4, for being cut to solid sample to be measured comprising removable and rotation cutter 41；
Dyeing apparatus 5, for being dyed to the sample in sample panel, interior coloring agent comprising pipeline and solid
Determine the connection of device 9；In the pipeline that dyeing apparatus is connected with fixed device, further includes: pump 8, for driving the input of coloring agent
Fixed device.
Imaging device 7 is used to shoot cleaved products, can be controlled by the control device；The imaging device
For photomicrographic device；The imaging device 7 includes camera lens 71；
Control device 6 controls the operation of fixed device 9, cutter device 4, dyeing apparatus 5 and imaging device 7.
Embodiment 3 cut to rice the example one that/dyeing/images using the device of embodiment 2
The normal rice of particle shape is chosen, after peelling off glume, is placed in sample panel 3, the horizontal and vertical difference of sample panel
If 4 and 11 holes.
With resin be embedding block of material, in the hole 2 that rice is put into sample panel 3 after, embedded block is placed in the four of rice grain
Week, for fixing rice.In embedding process, it is slowly injected into embedded material.
Rice after embedding clamps rice using clamping device 1, and clamping device is along 3 longitudinal edge of sample panel to rice grain
It is clamped.
Ultra-thin cutter 41 is set in cutter device 4, machine-cut is cut with a thickness of 0.1 millimeter, and cleaved products are transferred to sample
On product plate 3.In cutting process, the position of cutter Yu sample panel 3 is adjusted, makes it higher than about 0.1 millimeter of specimen holder.Cutter can
Rice surrounding with horizontal plane cut rice grain, completely cut rice entire section.In operation, complete to knife
Cheng Hou, feed keep the knife edge and sample close, and the mechanical arm where the mobile knife edge moves horizontally it, make the knife edge just from rice
It cuts in top cross section.
To rice cleaved products, the coloring agent in dyeing apparatus 5 is driven to be input in the container of fixed device 9, to sample
Cleaved products in plate 3 are dyed, and are observed under imaging device 7.
Pictorial diagram such as Fig. 4 institute using toluidine blue and sarranine aqueous solution as coloring agent, after the rice section dyeing of acquisition
It states.As it can be seen that obtaining the moderate image of high resolution, contrast.Image after dyeing can be directly used for seeing under microscope or current potential
It examines.
Embodiment 4 cut to rice the example one that/dyeing/images using the device of embodiment 2
If rice of the sample panel 3 for placement.The normal rice of particle shape is chosen, after peelling off glume, sample panel 3 can be placed in
In.Horizontal and vertical hole is set in sample panel 3.
It is embedding block of material with paraffin, embedded block is placed in the surrounding of rice grain after rice is put into, for fixing rice.
In embedding process, it is slowly injected into embedded material.
Fixed clamping device 1 is set in the fixation device 9 of rice, clamps rice grain using clamping device 1.It was fixing
Cheng Zhong, clamping device 1 are clamped rice grain along 3 transverse edge of sample panel, and mobile clamping device 1 arrives target position.
Ultra-thin cutter 41 is set in cutter device 4, machine-cut is cut with a thickness of 0.1 millimeter, and cleaved products are transferred to sample
On product plate 3.In cutting process, the position of cutter Yu sample panel 3 is adjusted, makes it higher than about 0.1 millimeter of specimen holder.Cutter can
Rice surrounding with horizontal plane cut rice grain, completely cut rice entire section.In operation, complete to knife
Cheng Hou, feed keep the knife edge and sample close, and the mechanical arm where the mobile knife edge moves horizontally it, make the knife edge just from rice
It cuts in top cross section.
To rice cleaved products, the coloring agent in dyeing apparatus 5 is driven to be input in the container of fixed device 9, to sample
Cleaved products in plate 3 are dyed, and are observed under imaging device 7.
In addition, it should also be understood that, after reading the above teachings of the present invention, those skilled in the art can be to this hair
Bright to make various changes or modifications, these equivalent forms also fall within the scope of the appended claims of the present application.
Claims (16)
1. a kind of device for solid sample to be measured to be cut and dyed characterized by comprising
Fixed device, is used to fix the sample panel of solid sample to be measured；
Cutter device, for being cut to solid sample to be measured comprising removable and rotation cutter；
Dyeing apparatus, comprising at least one coloring agent, for being dyed to the sample in sample panel；It includes at least one pipe
Road is connected to fixed device；
Control device controls the operation of fixed device, cutter device, dyeing apparatus.
2. the device as described in claim 1 for solid sample to be measured to be cut and dyed, which is characterized in that described
Fixation device in include: clamping device, for fixing the sample in sample panel.
3. the device as described in claim 1 for solid sample to be measured to be cut and dyed, which is characterized in that described
Fixation device can carry out the movement of horizontal direction and/or the movement of vertical direction.
4. the device as described in claim 1 for solid sample to be measured to be cut and dyed, which is characterized in that described
Sample panel be uniformly distributed grilled plate.
5. the device as claimed in claim 4 for solid sample to be measured to be cut and dyed, which is characterized in that described
Sample panel be evenly distributed with the grid of M × N, the positive integer that wherein M is 1~50；The positive integer that N is 1~50.
6. the device as described in claim 1 for solid sample to be measured to be cut and dyed, which is characterized in that described
Fixation device include that at least one is used to place the container of sample panel, the coloring agent exported from dyeing apparatus can be input to this
In container, to be dyed to the cleaved products in sample panel.
7. the device as claimed in claim 6 for solid sample to be measured to be cut and dyed, which is characterized in that described
Container further include a pipeline, for be discharged dyeing waste liquid.
8. the device as claimed in claim 7 for solid sample to be measured to be cut and dyed, which is characterized in that described
Pipeline in, further includes: pump, for drive dye waste liquid outflow.
9. the device as described in claim 1 for solid sample to be measured to be cut and dyed, which is characterized in that dyeing
In the pipeline that device is connected with fixed device, further includes: pump, for driving the fixed device of input of coloring agent.
10. the device as described in claim 1 for solid sample to be measured to be cut and dyed, which is characterized in that institute
The solid sample to be measured stated includes: vegetable seeds or it processes product, tissue sample.
11. the device as described in claim 1 for solid sample to be measured to be cut and dyed, which is characterized in that institute
The device stated further include: imaging device is used to shoot cleaved products, can be controlled by the control device.
12. the device as described in claim 1 for solid sample to be measured to be cut and dyed, which is characterized in that institute
The imaging device stated includes camera lens, controls focal length by the control device.
13. the application of any device of claim 1~12, which is characterized in that for being cut to solid sample to be measured
It cuts and dyes.
14. a kind of method that solid sample to be measured is cut and dyed using any device of claim 1~12,
It is characterized in that, which comprises
(1) solid sample to be measured is coated with embedded material, obtains the sample being coated；
(2) sample being coated that step (1) obtains is placed in sample panel, sample panel is fixed on fixed device；
(3) sample being coated is cut with cutter device, obtains cleaved products；
(4) coloring agent in dyeing apparatus is input to fixed device, the cleaved products in sample panel is dyed, are cut
The cleaved products being colored on face.
15. the method that solid sample to be measured is cut and dyed as claimed in claim 14, which is characterized in that the side
Method further include: with the section of cleaved products of the imaging device shooting through dyeing.
16. the method that solid sample to be measured is cut and dyed as claimed in claim 15, which is characterized in that described
Coloring agent includes: iodine solution or sarranine-crystal violet dye liquor or coomassie brilliant blue R250 dye liquor.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810311236.5A CN110361238A (en) | 2018-04-09 | 2018-04-09 | A kind of device and its application for cutting and dyeing |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810311236.5A CN110361238A (en) | 2018-04-09 | 2018-04-09 | A kind of device and its application for cutting and dyeing |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111487110A (en) * | 2020-04-28 | 2020-08-04 | 太原市罗塞塔石生物技术有限公司 | Gas circuit and liquid circuit integrated module for attaching glass slide |
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| US20180045623A1 (en) * | 2015-03-11 | 2018-02-15 | Timothy Ragan | Systems and methods for serial staining and imaging |
| CN208239156U (en) * | 2018-04-09 | 2018-12-14 | 中国科学院上海生命科学研究院 | A kind of device for cutting and dyeing |
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| CN111487110A (en) * | 2020-04-28 | 2020-08-04 | 太原市罗塞塔石生物技术有限公司 | Gas circuit and liquid circuit integrated module for attaching glass slide |
| CN111487110B (en) * | 2020-04-28 | 2023-01-31 | 太原市罗塞塔石生物技术有限公司 | Gas circuit and liquid circuit integrated module for attaching glass slide |
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