CN110358830A - Detect application and the kit of the reagent of No. 8 53 expressions of chromosome open reading frame - Google Patents
Detect application and the kit of the reagent of No. 8 53 expressions of chromosome open reading frame Download PDFInfo
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- CN110358830A CN110358830A CN201910623937.7A CN201910623937A CN110358830A CN 110358830 A CN110358830 A CN 110358830A CN 201910623937 A CN201910623937 A CN 201910623937A CN 110358830 A CN110358830 A CN 110358830A
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57415—Specifically defined cancers of breast
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/118—Prognosis of disease development
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
Abstract
The invention belongs to field of biotechnology, it is related to detecting application and the kit of the reagent of No. 8 53 expressions of chromosome open reading frame.Application of the reagent in preparation of the preparation for Computer-aided Diagnosis of Breast Cancer and/or patient with breast cancer's Index for diagnosis more particularly, to detection C8ORF53 expression and a kind of for Computer-aided Diagnosis of Breast Cancer and/or the kit of patient with breast cancer's Index for diagnosis.Clinical sample testing result shows that C8ORF53 expression is significantly increased compared with cancer beside organism in breast cancer；And C8ORF53 high expression is unfavorable for patient with breast cancer's overall survival.Therefore, the reagent for detecting the changes in gene expression can be used for Prognosis in Breast Cancer or diagnosis, treatment.
Description
Technical field
The invention belongs to field of biotechnology, more particularly, to No. 8 chromosome open reading frame 53 of detection
(C8ORF53) reagent of expression is in preparation for Computer-aided Diagnosis of Breast Cancer and/or the preparation of patient with breast cancer's Index for diagnosis
In application and a kind of for Computer-aided Diagnosis of Breast Cancer and/or the kit of patient with breast cancer's Index for diagnosis.
Background technique
Breast cancer is most commonly seen gynecologic malignant tumor.Global cancer epidemiology statistical data shows, the world in 2012
In range breast cancer new cases sum 1677000, be only second to lung cancer the second high-incidence tumour and women disease incidence and
The highest malignant tumour of the death rate.China's breast cancer incidence is up to 25.89/10 ten thousand, accounts for about all female malignant morbidities
The 16.83% of rate.Moreover, China's breast cancer illness number and new diagnosis patient's number are just being increasing year by year in global ratio, and
Show morbidity rejuvenation trend, the physical and mental health for seriously affecting women, even threat to life.Therefore, pathogenesis of breast carcinoma mechanism
Research with prognosis is of far-reaching significance to patient with breast cancer.Breast cancer is in origin of cell, Histological Study, disease classification, clinical table
Existing, therapeutic response and metastatic potential etc. all show great complexity and heterogeneity, limit Prognosis in Breast Cancer mark
The popularity of will object application.Therefore, there is an urgent need to develop more targeted prognostic marker for breast cancer, to meet clinical need
It asks.
With quickling increase for the high-throughput data of multiple groups, some molecular biology markers are found and mammary gland carcinogenesis
Related with prognosis, this makes it possible more acurrate, effectively diagnosis and treatment breast cancer.
Summary of the invention
The object of the present invention is to provide a kind of new No. 8 chromosome open reading frame 53 of prognostic marker for breast cancer
(C8ORF53), thus further provide for detection C8ORF53 expression reagent preparation for Computer-aided Diagnosis of Breast Cancer and/
Or application in the preparation of patient with breast cancer's Index for diagnosis and a kind of pre- for Computer-aided Diagnosis of Breast Cancer and/or patient with breast cancer
The kit judged afterwards.
C8ORF53 also known as UTP23 is a kind of protein coding gene, is positioned at 8q24.11, includes 3 exons, Gene
ID:84294, coding protein are primarily targeted for nucleus and cytoplasm.Currently, related C8ORF53 gene is sent out in breast cancer
Raw developing effect has not been reported.The present inventor passes through cancer and oncogene map (Cancer Genome
Atlas, TCGA) high throughput data mining discovery C8ORF53 high expression is unfavorable for patient with breast cancer's prognosis, and passes through collection clinic
Patient with breast cancer's sample and follow-up information further verify C8ORF53 differential expression to the Prognostic of patient with breast cancer.
To achieve the goals above, the first aspect of the present invention provides detection No. 8 chromosome open reading frame 53
(C8ORF53) reagent of expression is in preparation for Computer-aided Diagnosis of Breast Cancer and/or the preparation of patient with breast cancer's Index for diagnosis
In application.
Further, the detection C8ORF53 expression includes gene expression dose and/or the inspection for detecting C8ORF53
Survey the protein expression level of C8ORF53.
More specifically, the method for the detection C8ORF53 expression includes: to detect breast cancer by RT-qPCR method
With the expression quantity of C8ORF53 in cancer beside organism；C8ORF53mRNA in breast cancer and cancer beside organism is detected by molecular probe technology
Expression quantity；C8ORF53 expressing quantity in breast cancer and cancer beside organism is detected by immunohistochemistry or Western-Blot.
More specifically, the reagent of the detection C8ORF53 expression is the few core for targeting C8ORF53 DNA sequences encoding
Acid probe, PCR primer, or to target the antibody of C8ORF53.
In accordance with the present invention it is preferred that the reagent of the detection C8ORF53 expression is with SEQ ID NO:1 and SEQ
The real-time fluorescence quantitative PCR specific primer of nucleotide sequence shown in ID NO:2.
5 '-GCCTGGAGTTCCTCTCATGTT-3 ', SEQ ID NO:1；
5 '-CTGACCTGACTCCACTGCTT-3 ', SEQ ID NO:2.
The second aspect of the present invention provides a kind of examination for Computer-aided Diagnosis of Breast Cancer and/or patient with breast cancer's Index for diagnosis
Agent box, the kit include: the reagent for detecting C8ORF53 expression.
Further, the reagent of the detection C8ORF53 expression is the few core for targeting C8ORF53 DNA sequences encoding
Acid probe, PCR primer, or to target the antibody of C8ORF53.
Specifically, the reagent of the detection C8ORF53 expression is with SEQ ID NO:1 and SEQ ID NO:2 institute
Show the real-time fluorescence quantitative PCR specific primer of nucleotide sequence.
According to the present invention, the kit can also be containing other conventional reagents for real time fluorescent quantitative, preferably
Ground, the kit further includes at least one of following components: Trizol, isopropanol, chloroform, dehydrated alcohol, without RNA enzyme
Water, random primer, 5 × M-MLV buffer, dNTPs, RNase inhibitor, M-MLV reverse transcriptase, have SEQ ID NO:3 and
The ACTB real-time fluorescence quantitative PCR specific primer of nucleotide sequence shown in SEQ ID NO:4.
5 '-GGCACCCAGCACAATGAAGA-3 ', SEQ ID NO:3；
5 '-ACTCCTGCTTGCTGATCCAC-3 ', SEQ ID NO:4.
Clinical sample testing result shows that C8ORF53 expression significantly increases (P < 0.001) compared with cancer beside organism in breast cancer；
And C8ORF53 high expression is unfavorable for patient with breast cancer's overall survival (P=0.0155).Therefore, the changes in gene expression is detected
Reagent can be used for Prognosis in Breast Cancer or diagnosis, treatment.
Other features and advantages of the present invention will then part of the detailed description can be specified.
Detailed description of the invention
Exemplary embodiment of the invention is described in more detail in conjunction with the accompanying drawings, it is of the invention above-mentioned and its
Its purpose, feature and advantage will be apparent.
Fig. 1 shows the expression of C8ORF53 in TCGA high throughput data analysis breast cancer.C8ORF53 in breast cancer tissue
Expression is significantly higher than normal tissue (P < 0.001).
Fig. 2 shows TCGA high throughput data analysis C8ORF53 high to express the influence survived to patient with breast cancer.
C8ORF53 high expression is unfavorable for patient with breast cancer's overall survival (P=0.0127).
Fig. 3 shows the expression of C8ORF53 in breast cancer tissue.C8ORF53 expression is significantly higher than cancer in breast cancer tissue
Side tissue (P < 0.001).
Fig. 4 shows C8ORF53 high in breast cancer tissue and expresses the influence survived to patient with breast cancer.C8ORF53 high table
Up to being unfavorable for patient with breast cancer's overall survival (P=0.0155).
Specific embodiment
The preferred embodiment of the present invention is described in more detail below.Although the following describe preferred implementations of the invention
Mode, however, it is to be appreciated that may be realized in various forms the present invention without that should be limited by the embodiments set forth herein.It is real
The person that is not specified actual conditions in example is applied, is all carried out according to conventional conditions or manufacturer's recommended conditions.Agents useful for same or instrument are not
Production firm person is indicated, is the conventional products that can be obtained by commercially available purchase.
Embodiment 1
The present embodiment is used to illustrate the expression variation of C8ORF53 in TCGA high throughput data analysis breast cancer.
1.TCGA high throughput data analysis process:
Login TCGA portal website UALCAN (http://ualcan.path.uab.edu/index.html) homepage, point
It hits " Analysis ", inputs Gene Name " C8ORF53 ", select TCGA dataset " Breast invasive
Carcinoma ", retrieval are clicked " Expression ", and result is recorded.It is mapped using 12.0 software of GraphPad, statistical method
For T inspection, P < 0.05 is that difference is statistically significant.
2. result: the expression of C8ORF53 significantly increases (P < 0.001) compared with normal tissue in breast cancer tissue, such as Fig. 1 institute
Show.
Embodiment 2
The present embodiment is for illustrating that TCGA high throughput data analyze C8ORF53 high expression and Prognosis in Breast Cancer relationship.
1.TCGA high throughput data analysis process:
It logs in TCGA portal website cBioPortal (http://www.cbioportal.org/), selects tumour data set
" Breast Invasive Carcinoma (TCGA Provisional) ", group learn data and select " mRNA Expression z-
Scores (RNA Seq V2RSEM) ", gene input " C8ORF53:EXP >=2 ", it retrieves, is clicked in popup web page
" Survival " records result.Kaplan-Meier method draws survivorship curve, and log-rank examines survivorship curve difference, P <
0.05 is statistically significant for difference.
2. result: C8ORF53 high expression is unfavorable for patient with breast cancer's overall survival (P=0.0127) (Fig. 2).
Embodiment 3
The present embodiment is used to illustrate that the reagent of preparation detection C8ORF53 expression quantity to be used to prepare the examination of patient with breast cancer's prognosis
Agent box (50 secondary response).
1.Trizol 50.0ml；
2. isopropanol 50.0ml；
3. chloroform 50.0ml；
4. dehydrated alcohol 50.0ml；
5. without RNA enzyme water 5.0ml
6. 1.0 μM of 50.0 μ l of random primer (Random primers)；
7. 5 × M-MLV buffer 2.0ml；
10.0mM triphosphoric acid base deoxynucleotide 8. (dNTPs) 100.0 μ l；
9. 50.0 μ l of 40U/ μ l RNase inhibitor；
10. 50.0 μ l of 200U/ μ l M-MLV reverse transcriptase；
11. ABI 2×PCR Mix 2.0ml；
12. 10.0 μM of 30.0 μ l of C8ORF53 real-time fluorescence quantitative PCR specific primer, primer sequence are shown in Table 1；
13. 10.0 μM of 30.0 μ l of ACTB real-time fluorescence quantitative PCR specific primer, primer sequence are shown in Table 1.
1 fluorescence quantitative RT-PCR primer sequence of table
Embodiment 4
The present embodiment is used to illustrate the detection of clinical breast cancer tissue samples C8ORF53.
1. present study carries out under patient's informed consent.57 patient with breast cancer's clinical information are remembered from patient assessment
Record.Breast cancer sample is divided into two parts: a part is freezed immediately in liquid nitrogen, is stored in -80 DEG C until carrying out RNA extraction, separately
It is a part of then be used for histopathological evaluation.
2. Total RNAs extraction in tissue: this experiment carries out in ice bath.30~50mg is taken to organize (fresh or -70 DEG C and liquid nitrogen
The tissue of middle preservation) it sets in 1.5ml centrifuge tube, 1ml Trizol is added and is sufficiently homogenized, is stored at room temperature 5min；Every pipe is added
200 μ l chloroforms acutely mix 30sec, stand 15min, and 4 DEG C of 12000rpm are centrifuged 15min；400 μ l of gentle aspiration supernatant liquid
To in another new centrifuge tube, isometric isopropanol is added, is gently mixed by inversion, 4 DEG C of 12000rpm are centrifuged 10min；Supernatant is abandoned,
75% ethanol wash sediment of 1ml is added, 4 DEG C of 12000rpm are centrifuged 10min；Supernatant is discarded as far as possible, is dried at room temperature
10min, every pipe are added 10 μ l without RNA enzyme water, dissolve (65 DEG C of dissolution 10-15min).OD260 is measured, RNA concentration is calculated.
RNA (mg/ml)=40 × OD260× extension rate (n)/1000
3. reverse transcription: every 25 μ l reverse transcription system includes 100pmol random primer, 2 μ g total serum IgEs, 1 μ of M-MLV reverse transcriptase
0.625 1.25 μ l, 5 × M-MLV buffer of μ l, dNTPs (10mM) of l, RNase inhibitor, 5 μ l, no RNA enzyme water polishing to 25 μ l.
Reaction condition are as follows: 37 DEG C of 1h, 95 DEG C of 5min.
4. quantitative PCR: every 20 μ l reaction system includes 2 × PCR Mix, 10 μ l, each 0.4 μ l, cDNA 1 of upstream and downstream primer
μ l, ddH2O 8.2μl.Reaction condition are as follows: 94 DEG C of 2min, 94 DEG C of 15s, 60 DEG C of 40s, 40 circulations.
5.2-ΔΔCTMethod calculates C8ORF53 relative expression quantity: this experiment detects 57 breast cancer tissues and 18 cancer beside organisms
The relative expression quantity of middle C8ORF53 changes.ACTB is as reference gene, the target gene C8ORF53C that qPCR is measuredTIt is worth and same
The C of tissue-derived reference gene ACTBTValue subtracts each other to obtain Δ CT, then by Δ CTWith control group Δ CTSubtract each other to obtain Δ Δ CTIt (takes
Sample Δ C by cancerTAverage value be Δ CTControl), every group of C8ORF53 is calculated with respect to table using Power function in Excell table
Up to amount.It is drawn using software GraphPad Prism 6.0, C8ORF53 differential expression by T check analysis breast cancer and cancer, P <
0.05 has statistical significance for difference.
6.C8ORF53 high expression and patient with breast cancer's prognosis: follow-up of patients's time is 1-32 months, successfully receives follow-up trouble
Person's number is 57.It is that height is expressed that C8ORF53 relative expression quantity, which is higher than 2 times of cancer beside organism's relative expression quantity mean, totally 13,
He is C8ORF53 low expression, totally 44.Kaplan-Meier method draws survivorship curve, and log-rank examines survivorship curve difference,
P < 0.05 is that difference is statistically significant.
7. result:
Clinical sample testing result shows that C8ORF53 expression significantly increases (P < 0.001) compared with cancer beside organism in breast cancer,
As shown in Figure 3；And C8ORF53 high expression is unfavorable for patient with breast cancer's overall survival (P=0.0155), as shown in Figure 4.
Various embodiments of the present invention are described above, above description is exemplary, and non-exclusive, and
It is not limited to disclosed each embodiment.Without departing from the scope and spirit of illustrated each embodiment, for this skill
Many modifications and changes are obvious for the those of ordinary skill in art field.
Sequence table
<110>Jiangsu medical profession institute
<120>application and the kit of the reagent of No. 8 53 expressions of chromosome open reading frame are detected
<130> BJI1900776JSYY
<160> 4
<170> SIPOSequenceListing 1.0
<210> 1
<211> 21
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 1
gcctggagtt cctctcatgt t 21
<210> 2
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 2
ctgacctgac tccactgctt 20
<210> 3
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 3
ggcacccagc acaatgaaga 20
<210> 4
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 4
actcctgctt gctgatccac 20
Claims (9)
1. the reagent for detecting No. 8 chromosome open reading frame 53 (C8ORF53) expressions is examined in preparation for breast cancer auxiliary
Application in the disconnected and/or preparation of patient with breast cancer's Index for diagnosis.
2. application according to claim 1, wherein the detection C8ORF53 expression includes the base for detecting C8ORF53
Because of expression and/or the protein expression level of detection C8ORF53.
3. application according to claim 1, wherein the method for the detection C8ORF53 expression includes: to pass through RT-
QPCR method detects the expression quantity of C8ORF53 in breast cancer and cancer beside organism；Breast cancer and cancer are detected by molecular probe technology
C8ORF53 mrna expression amount in the tissue of side；It is detected in breast cancer and cancer beside organism by immunohistochemistry or Western-Blot
C8ORF53 expressing quantity.
4. application according to claim 1, wherein the reagent of the detection C8ORF53 expression is targeting C8ORF53
The oligonucleotide probe of DNA sequences encoding, PCR primer, or to target the antibody of C8ORF53.
5. application according to claim 4, wherein the reagent of the detection C8ORF53 expression is with SEQ ID
The real-time fluorescence quantitative PCR specific primer of nucleotide sequence shown in NO:1 and SEQ ID NO:2.
6. a kind of for Computer-aided Diagnosis of Breast Cancer and/or the kit of patient with breast cancer's Index for diagnosis, which includes: detection
The reagent of C8ORF53 expression.
7. kit according to claim 6, wherein the reagent of the detection C8ORF53 expression is targeting
The oligonucleotide probe of C8ORF53 DNA sequences encoding, PCR primer, or to target the antibody of C8ORF53.
8. kit according to claim 7, wherein the reagent of the detection C8ORF53 expression is with SEQ
The real-time fluorescence quantitative PCR specific primer of nucleotide sequence shown in ID NO:1 and SEQ ID NO:2.
9. kit according to claim 6, wherein the kit further includes at least one of following components:
Trizol, isopropanol, chloroform, dehydrated alcohol, without RNA enzyme water, random primer, 5 × M-MLV buffer, dNTPs, RNA enzyme inhibit
Agent, M-MLV reverse transcriptase, the ACTB real time fluorescent quantitative with nucleotide sequence shown in SEQ ID NO:3 and SEQ ID NO:4
PCR specific primer.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109563547A (en) * | 2016-04-15 | 2019-04-02 | 外来体诊断公司 | The detection based on blood plasma of anaplastic lymphoma kinase (ALK) nucleic acid and ALK fusion transcript and its purposes in cancer diagnosis and treatment |
| CN109797219A (en) * | 2019-01-08 | 2019-05-24 | 江苏医药职业学院 | Detect application and the kit of the reagent of ABRACL expression |
-
2019
- 2019-07-11 CN CN201910623937.7A patent/CN110358830B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109563547A (en) * | 2016-04-15 | 2019-04-02 | 外来体诊断公司 | The detection based on blood plasma of anaplastic lymphoma kinase (ALK) nucleic acid and ALK fusion transcript and its purposes in cancer diagnosis and treatment |
| CN109797219A (en) * | 2019-01-08 | 2019-05-24 | 江苏医药职业学院 | Detect application and the kit of the reagent of ABRACL expression |
Non-Patent Citations (1)
| Title |
|---|
| ISMAHANE BEKHOUCHE等: "High-resolution comparative genomic hybridization of inflammatory breast cancer and identification of candidate genes", 《PLOS ONE》 * |
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