CN110358828B - Application of reagent for detecting expression level of E3 SUMO protein transferase NSE2 and kit - Google Patents
Application of reagent for detecting expression level of E3 SUMO protein transferase NSE2 and kit Download PDFInfo
- Publication number
- CN110358828B CN110358828B CN201910615525.9A CN201910615525A CN110358828B CN 110358828 B CN110358828 B CN 110358828B CN 201910615525 A CN201910615525 A CN 201910615525A CN 110358828 B CN110358828 B CN 110358828B
- Authority
- CN
- China
- Prior art keywords
- breast cancer
- nsmce2
- detecting
- expression level
- expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57415—Specifically defined cancers of breast
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/118—Prognosis of disease development
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Hospice & Palliative Care (AREA)
- Cell Biology (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Oncology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention belongs to the technical field of biology, and relates to application of a reagent for detecting the expression level of E3 SUMO protein transferase NSE2 and a kit. More particularly, the application of the reagent for detecting the expression level of NSMCE2 in the preparation of a preparation for breast cancer auxiliary diagnosis and/or breast cancer patient prognosis and a kit for breast cancer auxiliary diagnosis and/or breast cancer patient prognosis. The clinical sample detection result shows that the NSMCE2 expression in the breast cancer is obviously increased compared with that in the tissues beside the cancer; and high expression of NSMCE2 is not beneficial to the overall survival of breast cancer patients. Therefore, the reagent for detecting the change of the gene expression can be used for the prognosis or diagnosis and treatment of the breast cancer.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to application of a reagent for detecting the expression level of E3 SUMO protein transferase NSE2(NSMCE2) in preparation of a preparation for auxiliary diagnosis of breast cancer and/or prognosis judgment of a breast cancer patient, and a kit for auxiliary diagnosis of breast cancer and/or prognosis judgment of the breast cancer patient.
Background
Breast cancer is the most common gynecological malignancy. Global tumor epidemiological data show that 1677000 new cases of breast cancer are second to lung cancer in total 1677000 worldwide in 2012, and are the highest malignant tumors in women with highest morbidity and mortality. The incidence rate of breast cancer in China is up to 25.89/10 ten thousand, and accounts for about 16.83 percent of the incidence rate of malignant tumors of all women. Moreover, the proportion of the number of breast cancer patients and the number of newly diagnosed patients in China is increasing year by year, and the patients show a trend of younger onset, which seriously affects the physical and mental health of women and even endangers life. Therefore, the study of pathogenesis and prognosis of breast cancer is of far-reaching importance to breast cancer patients. The breast cancer shows great complexity and heterogeneity in the aspects of cell origin, histological morphology, disease grading, clinical representation, treatment response, metastatic potential and the like, and the application of the breast cancer prognosis marker is limited. Therefore, there is an urgent need to develop more targeted breast cancer prognostic markers to meet clinical needs.
With the rapid increase of multigroup high-throughput data, some molecular biological markers are found to be related to the occurrence and prognosis of breast cancer, which makes it possible to diagnose breast cancer more accurately and effectively.
Disclosure of Invention
The invention aims to provide a novel breast cancer prognostic marker E3 SUMO protein transferase NSE2(NSMCE2), and further provides application of a reagent for detecting the expression level of NSMCE2 in preparation of a preparation for breast cancer auxiliary diagnosis and/or breast cancer patient prognosis and a kit for breast cancer auxiliary diagnosis and/or breast cancer patient prognosis.
NSMCE2, also known as MMS21, is a protein-encoding gene, located at 8q24.13, containing 15 exons, GeneID:286053, which encodes a protein that is predominantly located in the nucleus and mitochondria. At present, the function of NSMCE2 gene in the occurrence and development of breast cancer is not reported. The inventor discovers that NSMCE2 high expression is unfavorable for the prognosis of breast Cancer patients through high-throughput data mining of Cancer and tumor gene maps (TCGA), and further verifies the prognostic effect of NSMCE2 expression difference on the breast Cancer patients through collecting clinical breast Cancer patient samples and follow-up information.
In order to achieve the above object, the first aspect of the present invention provides a use of a reagent for detecting an expression level of E3 SUMO protein transferase NSE2(NSMCE2) in preparing a preparation for breast cancer auxiliary diagnosis and/or breast cancer patient prognosis.
Further, the detecting the expression level of the NSMCE2 comprises detecting the expression level of genes of NSMCE2 and/or detecting the expression level of proteins of NSMCE 2.
More specifically, the method for detecting the expression level of NSMCE2 comprises the following steps: detecting the expression quantity of NSMCE2 in breast cancer and paracarcinoma tissues by an RT-qPCR method; detecting NSMCE2mRNA expression level in breast cancer and tissues beside the breast cancer by a molecular probe technology; the expression level of NSMCE2 protein in breast cancer and paracarcinoma tissues is detected by immunohistochemistry or Western-Blot.
More specifically, the reagent for detecting the expression level of NSMCE2 is an oligonucleotide probe targeting NSMCE2 coding DNA sequence, a PCR primer or an antibody targeting NSMCE 2.
According to the present invention, preferably, the reagent for detecting the expression level of NSMCE2 is a reagent having an amino acid sequence shown in SEQ ID NO: 1 and SEQ ID NO: 2 in the presence of a primer for real-time fluorescent quantitative PCR.
5’-GTGTTGCTTTGGATCTTGTGGAA-3’，SEQ ID NO：1；
5’-TGCCGATCCAATGTAGCAAA-3’，SEQ ID NO：2。
The second aspect of the present invention provides a kit for breast cancer auxiliary diagnosis and/or breast cancer patient prognosis, which comprises: an agent for detecting the expression level of NSMCE 2.
Further, the reagent for detecting the expression level of NSMCE2 is an oligonucleotide probe targeting NSMCE2 coding DNA sequence, a PCR primer or an antibody targeting NSMCE 2.
Specifically, the reagent for detecting the expression level of NSMCE2 is a reagent with the sequence shown in SEQ ID NO: 1 and SEQ ID NO: 2 in the presence of a primer for real-time fluorescent quantitative PCR.
According to the present invention, the kit may further comprise other conventional reagents for real-time fluorescence quantification, and preferably, the kit further comprises at least one of Trizol, isopropanol, chloroform, absolute ethanol, RNase-free water, random primers, 5 × M-MLV buffer, dNTPs, RNase inhibitor, M-MLV reverse transcriptase, ACTB real-time fluorescence quantification PCR specific primers having nucleotide sequences shown in SEQ ID NO. 3 and SEQ ID NO. 4.
5’-GGCACCCAGCACAATGAAGA-3’，SEQ ID NO：3；
5’-ACTCCTGCTTGCTGATCCAC-3’，SEQ ID NO：4。
The clinical sample detection result shows that the expression of NSMCE2 in the breast cancer is obviously increased compared with that in the para-carcinoma tissues (P < 0.001); and high expression of NSMCE2 is detrimental to overall survival of breast cancer patients (P0.0339). Therefore, the reagent for detecting the change of the gene expression can be used for the prognosis or diagnosis and treatment of the breast cancer.
Additional features and advantages of the invention will be set forth in the detailed description which follows.
Drawings
The above and other objects, features and advantages of the present invention will become more apparent by describing in more detail exemplary embodiments thereof with reference to the attached drawings.
Figure 1 shows TCGA high throughput data analysis of the expression of NSMCE2 in breast cancer. NSMCE2 expression in breast cancer tissues was significantly higher than in normal tissues (P < 0.001).
Figure 2 shows TCGA high throughput data analysis of the effect of high expression of NSMCE2 on survival of breast cancer patients. High expression of NSMCE2 is detrimental to overall survival in breast cancer patients (P0.005272).
Figure 3 shows the expression of NSMCE2 in breast cancer tissue. The expression of NSMCE2 in breast cancer tissues was significantly higher than in paracarcinoma tissues (P < 0.001).
Figure 4 shows the effect of high expression of NSMCE2 in breast cancer tissue on survival of breast cancer patients. High expression of NSMCE2 is detrimental to overall survival in breast cancer patients (P0.0339).
Detailed Description
Preferred embodiments of the present invention will be described in more detail below. While the following describes preferred embodiments of the present invention, it should be understood that the present invention may be embodied in various forms and should not be limited by the embodiments set forth herein. The examples, in which the specific conditions are not specified, were conducted under the conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
Example 1
This example demonstrates TCGA high throughput data analysis of the change in expression of NSMCE2 in breast cancer.
TCGA high throughput data analysis process:
log-in TCGA Portal website UALCAN ((TM))http://ualcan.path.uab.edu/index.html) First page, click on "Analysis", enter the gene name "NSMCE 2", select TCGA dataset "Breast invasivechicoma", search, click on "Expression", record the knotAnd (5) fruit. And (3) mapping by using GraphPad 12.0 software, wherein the statistical method is T test, and the difference is statistically significant when P is less than 0.05.
2. As a result: the expression of NSMCE2 was significantly elevated in breast cancer tissues compared to normal tissues (P <0.001), as shown in figure 1.
Example 2
This example illustrates the relationship between high expression of NSMCE2 and breast cancer prognosis in TCGA high throughput data analysis.
TCGA high throughput data analysis process:
the TCGA Portal site cBioPortal (http:// www.cbioportal.org /), tumor dataset "Breast Invasive Carcinoma (TCGA Provisional)" was selected, omics dataset "mRNA Expression z-scenes (RNA Seq V2 RSEM)" was selected, gene input "NSMCE 2: and EXP > 2 ', searching, clicking ' survivval ' in a pop-up page, and recording a result. The Kaplan-Meier method draws a survival curve, and log-rank tests the difference of the survival curve, wherein the difference has statistical significance when P is less than 0.05.
2. As a result: high expression of NSMCE2 was detrimental to overall survival in breast cancer patients (P0.005272) (fig. 2).
Example 3
This example illustrates the preparation of reagents for detecting NSMCE2 expression levels for the preparation of a kit for prognosis of breast cancer patients (50 reactions).
1.Trizol 50.0ml；
2. 50.0ml of isopropanol;
3. 50.0ml of chloroform;
4. 50.0ml of absolute ethyl alcohol;
5. RNase-free water 5.0ml
6.1.0. mu.M Random primers (Random primers) 50.0. mu.l;
7.5 × M-MLV buffer 2.0 ml;
8.10.0 mM deoxynucleotide triphosphate (dNTPs) 100.0. mu.l;
9.40U/. mu.l RNase inhibitor 50.0. mu.l;
10.200U/. mu. l M-MLV reverse transcriptase 50.0. mu.l;
11.ABI 2×PCR Mix 2.0ml；
12.10.0 μ M NSMCE2 real-time fluorescent quantitative PCR specific primer 30.0 μ l, the primer sequence is shown in Table 1;
13.10.0 μ M ACTB real-time fluorescent quantitation PCR specific primers 30.0 μ l, the primer sequences are shown in Table 1.
TABLE 1 fluorescent quantitative RT-PCR primer sequences
Example 4
This example illustrates the detection of clinical breast cancer tissue sample NSMCE 2.
1. The study was performed with patient informed consent. Clinical information from 57 breast cancer patients was obtained from patient visit records. Breast cancer specimens are divided into two parts: one fraction was immediately frozen in liquid nitrogen and stored at-80 ℃ until RNA extraction, and the other fraction was used for histopathological evaluation.
2. Extraction of total RNA from tissues: the experiment was performed in an ice bath. Placing 30-50 mg of tissue (fresh or tissue preserved at-70 ℃ in liquid nitrogen) into a 1.5ml centrifuge tube, adding 1ml Trizol, homogenizing fully, and standing at room temperature for 5 min; adding 200 μ l chloroform into each tube, mixing vigorously for 30sec, standing for 15min, and centrifuging at 4 deg.C and 12000rpm for 15 min; gently sucking 400 μ l of the supernatant liquid into another new centrifuge tube, adding isopropanol with the same volume, gently inverting and mixing, and centrifuging at 12000rpm at 4 ℃ for 10 min; discarding the supernatant, adding 1ml of 75% alcohol to wash the precipitate, and centrifuging at 4 ℃ and 12000rpm for 10 min; the supernatant was discarded as much as possible, air-dried at room temperature for 10min, and dissolved by adding 10. mu.l of RNase-free water to each tube (10-15 min. lysis-promoting at 65 ℃). OD260 was measured and the RNA concentration was calculated.
RNA(mg/ml)＝40×OD260× dilution multiple (n)/1000
3. Reverse transcription, each 25. mu.l reverse transcription system comprises 100pmol random primer, 2. mu.g total RNA, 1. mu.l M-MLV reverse transcriptase, 0.625. mu.l RNase inhibitor, 1.25. mu.l dNTPs (10mM), 5. mu.l 5 × M-MLV buffer, no RNase water is added to 25. mu.l, reaction conditions are 37 ℃ for 1h, 95 ℃ for 5 min.
4. Quantitative PCR, each 20. mu.l reaction system contained 2 × PCR Mix 10. mu.l, upstream and downstream primers 0.4. mu.l each, cDNA 1. mu.l, ddH2O8.2. mu.l. The reaction conditions are as follows: 94 ℃ for 2min, 94 ℃ for 15s, 60 ℃ for 40s, 40 cycles.
5.2-ΔΔCTCalculating the relative expression of NSMCE 2: the experiment detects the relative expression change of NSMCE2 in 57 breast cancer tissues and 18 paracarcinoma tissues. ACTB as reference gene, and the target gene NSMCE2C measured by qPCRTC of reference gene ACTB with the same value as that of the tissue sourceTSubtracting the values to obtain Δ CTThen, Δ C is addedTAnd control group Δ CTSubtracting to obtain Δ Δ CT(taking a paracarcinoma sample Δ CTHas an average value of Δ CTControl), the relative expression of NSMCE2 in each group was calculated using the Power function in the Excell table. Differential expression of NSMCE2 between breast and paracarcinoma, P, was analyzed using a software GraphPad Prism 6.0 mapping, T test<A difference of 0.05 is statistically significant.
High expression of NSMCE2 in patients with breast cancer: the follow-up time of the patients is 1-32 months, and the number of successfully received follow-up patients is 57. The relative expression of NSMCE2 was high when the relative expression was 2 times higher than the average relative expression of the para-carcinoma tissues, and 20 cases were high, while the other cases were low in NSMCE2 and 37 cases were high. The Kaplan-Meier method draws a survival curve, and log-rank tests the difference of the survival curve, wherein the difference has statistical significance when P is less than 0.05.
7. As a result:
the clinical sample detection result shows that the expression of NSMCE2 in the breast cancer is obviously increased compared with that in the para-carcinoma tissue (P <0.001), as shown in figure 3; and high expression of NSMCE2 is detrimental to overall survival of breast cancer patients (P0.0339), as shown in fig. 4.
Having described embodiments of the present invention, the foregoing description is intended to be exemplary, not exhaustive, and not limited to the embodiments disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments.
Sequence listing
<110> Jiangsu medical profession college
<120> application of reagent for detecting expression level of E3 SUMO protein transferase NSE2 and kit
<130>BJI1900773JSYY
<160>4
<170>SIPOSequenceListing 1.0
<210>1
<211>23
<212>DNA
<213> Artificial Sequence (Artificial Sequence)
<400>1
gtgttgcttt ggatcttgtg gaa 23
<210>2
<211>20
<212>DNA
<213> Artificial Sequence (Artificial Sequence)
<400>2
<210>3
<211>20
<212>DNA
<213> Artificial Sequence (Artificial Sequence)
<400>3
<210>4
<211>20
<212>DNA
<213> Artificial Sequence (Artificial Sequence)
<400>4
Claims (5)
1. Use of an agent for detecting the expression level of NSMCE2 in the preparation of a formulation for the prognostic determination of a breast cancer patient.
2. The use of claim 1, wherein said detecting the expression level of NSMCE2 comprises detecting the expression level of a gene of NSMCE2 and/or detecting the expression level of a protein of NSMCE 2.
3. The use according to claim 1, wherein the method for detecting the expression level of NSMCE2 comprises: detecting the expression quantity of NSMCE2 in breast cancer and paracarcinoma tissues by an RT-qPCR method; detecting NSMCE2mRNA expression level in breast cancer and cancer adjacent tissues by a molecular probe technology; the expression level of NSMCE2 protein in breast cancer and paracarcinoma tissues is detected by immunohistochemistry or Western-Blot.
4. The use of claim 1, wherein the agent for detecting the expression level of NSMCE2 is an oligonucleotide probe targeting a DNA sequence encoding NSMCE2, a PCR primer, or an antibody targeting NSMCE 2.
5. The use of claim 4, wherein the reagent for detecting the expression level of NSMCE2 is SEQ ID NO: 1 and SEQ ID NO: 2 in the presence of a primer for real-time fluorescent quantitative PCR.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910615525.9A CN110358828B (en) | 2019-07-09 | 2019-07-09 | Application of reagent for detecting expression level of E3 SUMO protein transferase NSE2 and kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910615525.9A CN110358828B (en) | 2019-07-09 | 2019-07-09 | Application of reagent for detecting expression level of E3 SUMO protein transferase NSE2 and kit |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110358828A CN110358828A (en) | 2019-10-22 |
| CN110358828B true CN110358828B (en) | 2020-07-03 |
Family
ID=68218854
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910615525.9A Active CN110358828B (en) | 2019-07-09 | 2019-07-09 | Application of reagent for detecting expression level of E3 SUMO protein transferase NSE2 and kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110358828B (en) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013003384A1 (en) * | 2011-06-27 | 2013-01-03 | Dana-Farber Cancer Institute, Inc. | Signatures and determinants associated with prostate cancer progression and methods of use thereof |
| CN108753967B (en) * | 2018-06-08 | 2022-06-17 | 复旦大学附属中山医院 | Gene set for liver cancer detection and panel detection design method thereof |
| CN109797219B (en) * | 2019-01-08 | 2019-12-13 | 江苏医药职业学院 | Application of reagent for detecting ABRACL expression level and kit |
-
2019
- 2019-07-09 CN CN201910615525.9A patent/CN110358828B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN110358828A (en) | 2019-10-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109797219B (en) | Application of reagent for detecting ABRACL expression level and kit | |
| EP2664679A1 (en) | Gene expression profiles to predict breast cancer outcomes | |
| CN108949992B (en) | Biomarker related to esophageal squamous carcinoma and grading thereof | |
| CN110273000B (en) | Application of reagent for detecting expression level of zinc finger protein 468 and kit | |
| CN111041095B (en) | Application of reagent for detecting expression level of chromosome 8 open reading frame 73 and kit | |
| CN108866187B (en) | Long-chain non-coding RNA marker related to lung cancer auxiliary diagnosis and application thereof | |
| CN109852698B (en) | Application of reagent for detecting ring finger protein 32 expression level and kit | |
| CN106755343A (en) | Cancer of pancreas Prognosis molecular marked compound | |
| CN111041098B (en) | Application of reagent for detecting proline-rich and frizzled 2A expression level and kit | |
| CN111041093B (en) | Application of reagent for detecting expression level of coiled coil domain protein 127 and kit | |
| CN110358828B (en) | Application of reagent for detecting expression level of E3 SUMO protein transferase NSE2 and kit | |
| CN111041092A (en) | Application of reagent for detecting expression level of Fas associated factor family member 2 and kit | |
| CN110358830B (en) | Application of reagent for detecting expression level of open reading frame 53 of chromosome 8 and kit | |
| CN111041097A (en) | Application of reagent for detecting expression level of open reading frame 76 of chromosome 8 and kit | |
| CN110358831B (en) | Application of reagent for detecting expression level of transmembrane protein 41A and kit | |
| CN110527727B (en) | Application of reagent for detecting zinc finger protein 28 expression level and kit | |
| CN110358832B (en) | Application of reagent for detecting expression level of PH domain family A member 6 and kit | |
| CN111041099B (en) | Application of reagent for detecting expression level of G protein-coupled receptor 137B and kit | |
| CN111041091B (en) | Application of reagent for detecting expression level of THUMP structural domain protein 3 and kit | |
| CN111041094B (en) | Application of reagent for detecting expression level of TM2 structural domain protein 2 and kit | |
| CN110358833B (en) | Application of reagent for detecting expression level of Tudor structural domain protein 2 and kit | |
| CN110358829A (en) | Detect application and the kit of the reagent of recombined human peptidyl prolyl cis-trans isomerase-H expression | |
| CN111041096A (en) | Application of reagent for detecting expression level of open reading frame 33 of chromosome 8 and kit | |
| CN111041100A (en) | Application of reagent for detecting expression level of tetraspanin 17 and kit | |
| CN114990217A (en) | Application of reagent for detecting expression level of chromosome 17 open reading frame 42 in skin melanoma |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220525 Address after: No.1 Huanghai Road, Shuiji sub district office, Laixi City, Qingdao City, Shandong Province 266600 Patentee after: Qingdao Yanding Biomedical Technology Co.,Ltd. Address before: 2081, building a, 88 Jianghai West Road, Liangxi District, Wuxi City, Jiangsu Province, 214000 Patentee before: Wuxi Xiangyuan Information Technology Co.,Ltd. Effective date of registration: 20220525 Address after: 2081, building a, 88 Jianghai West Road, Liangxi District, Wuxi City, Jiangsu Province, 214000 Patentee after: Wuxi Xiangyuan Information Technology Co.,Ltd. Address before: 224008 no.283, Jiefang South Road, Yancheng City, Jiangsu Province Patentee before: JIANGSU VOCATIONAL College OF MEDICINE |
|
| TR01 | Transfer of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Application of reagents and kits for detecting the expression level of E3 SUMOylation protein transferase NSE2 Effective date of registration: 20230721 Granted publication date: 20200703 Pledgee: Weihai commercial bank Limited by Share Ltd. Qingdao branch Pledgor: Qingdao Yanding Biomedical Technology Co.,Ltd. Registration number: Y2023980049237 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |