CN110358670B - Liquid fermentation process of soil remediation type microbial bacterium combination - Google Patents

Liquid fermentation process of soil remediation type microbial bacterium combination Download PDF

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Publication number
CN110358670B
CN110358670B CN201910787605.2A CN201910787605A CN110358670B CN 110358670 B CN110358670 B CN 110358670B CN 201910787605 A CN201910787605 A CN 201910787605A CN 110358670 B CN110358670 B CN 110358670B
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fermentation
device body
fermentation device
cooling
liquid
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CN110358670A (en
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李西强
冷鹏
全先庆
崔爱华
宿刚爱
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Shandong Jinhongyuan Ecological Agriculture Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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Abstract

The invention discloses a liquid fermentation process of soil remediation type microbial bacteria combination, wherein a feed inlet for strain combination liquid to enter is arranged at the upper part of a fermentation device body, the strain combination liquid enters the fermentation device body, then a motor forward and backward rotation automatic controller below the fermentation device body controls a motor to rotate back and forth between certain angles, and the strain combination liquid is stirred to be uniformly distributed and rapidly fermented; when the strain combined liquid in fermentation needs to be cooled, cooling water is added into the cooling device through a plurality of water inlets arranged at the lower end of the fermentation device body, the cooling water passes through a rotary cooling pipe and a central cooling ring cold water cavity in the fermentation device body and then is discharged through a water outlet at the upper part of the fermentation device body, and the fermentation degree of the strain combined liquid is observed; the rotary cooling pipe designed by the invention rotates back and forth under the action of the rotating shaft, so that a rotary stirring and cooling integrated structure is realized, the space is saved, the working efficiency of the fermentation device is improved, and the external pollution is avoided.

Description

Liquid fermentation process of soil remediation type microbial bacterium combination
Technical Field
The invention relates to the technical field of biological fermentation, in particular to a liquid fermentation process of soil remediation type microbial bacteria combination.
Background
The strain combination liquid is a liquid culture medium, and the edible fungus strain in a liquid form is produced in a biological fermentation tank by a liquid fermentation technology, wherein the liquid refers to the physical state of the culture medium, and the liquid submerged culture is a fermentation engineering technology. In the technical field of strain liquid fermentation, stirring and cooling are both used for improving the fermentation rate of strains, but in the existing strain liquid fermentation technology, stirring is generally manual or simple equipment stirring, the mode is easy to interfere with appearance factors such as bacteria and the like in a fermentation device body, so that the fermentation environment is polluted, and meanwhile, a stirring device and a cooling device are independent, so that the occupied space is large, and the operation is troublesome; therefore, the liquid fermentation process integrating stirring and cooling and rapidly realizing the combination of soil remediation type microorganisms is a problem which needs to be solved urgently.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide a liquid fermentation process of a soil remediation type microbial bacteria combination.
The technical scheme adopted by the invention for solving the technical problems is as follows: a liquid fermentation process of soil restoration type microorganism bacterium combination comprises
The fermentation device comprises a fermentation device body and a fermentation carrier of strain combination liquid;
the cooling device is used for providing circulating cooling water for the fermentation device body and cooling the strain combined liquid;
the sampling device is used for sucking the strain combined liquid in the fermentation process from the fermentation device body and is used for observing the fermentation degree;
the upper part of the fermentation device body is provided with a feed inlet for the entry of the strain combined liquid, the strain combined liquid enters the fermentation device body, then the motor is controlled by the motor forward and reverse rotation automatic controller below the fermentation device body to rotate back and forth between certain angles, the motor drives the rotary cooling pipe of the cooling device to rotate back and forth, and the strain combined liquid is stirred to be uniformly distributed and rapidly fermented;
when the strain combination liquid in fermentation needs to be cooled, cooling water is added into the cooling device through a plurality of water inlets arranged at the lower end of the fermentation device body, and the cooling water passes through a cold water cavity formed by a rotary cooling pipe, a central cooling ring and the fermentation device body in the fermentation device body, is discharged through a water outlet at the upper part of the fermentation device body and enters a circulating system;
in the fermentation process, the sampling device absorbs the strain combination liquid in the fermentation from the fermentation device body to the sampling bottle by utilizing the siphon principle, and is used for observing the fermentation degree of the strain combination liquid.
Specifically, the strain combined liquid fermented in the fermentation device body is collected through a discharge hole formed in the lower part of the fermentation device body.
Specifically, a plurality of conical barrel-shaped agitators with the functions of buffering and storing water are arranged on the rotary cooling pipe, and power is increased when strain combined liquid is agitated.
Specifically, during the fermentation process of the strain combined liquid, the environment in the fermentation device body is monitored in real time through a display device of a temperature and humidity sensor arranged on the upper part of the fermentation device body, and corresponding adjustment is made.
Specifically, after the fermentation process of the strain combined liquid is completed, sterile clear water is added through a feeding hole in the upper part of the fermentation device body, the fermentation device body is stirred and cleaned by a motor, and then waste water is discharged through a discharging hole.
The invention has the following beneficial effects:
the rotary cooling pipe designed by the invention rotates back and forth under the action of the motor and the motor forward and backward rotation automatic controller, so that a rotary stirring and cooling integrated structure is realized, the space is saved, the working efficiency of the fermentation process is improved, and the external pollution is avoided; the designed stirrer is in a conical cylinder shape, plays roles of buffering and storing water, increases the contact area of cooling water and strain combined liquid, and increases stirring power to ensure that stirring is more uniform and powerful; the designed sampling device adopts a siphon principle, so that the fermentation process is energy-saving and environment-friendly; the designed temperature and humidity sensor can display the working state inside the fermentation device body in real time, and is convenient to adjust, so that the fermentation process is more stable and accurate.
Drawings
FIG. 1 is a flow chart of a liquid fermentation process of a soil remediation type microbial consortium.
FIG. 2 is a schematic structural diagram of an example of a liquid fermentation process of a soil remediation type microbial consortium.
In the figure: 1-a fermentation device body; 2-a cooling device; 3-a sampling device; 4-a temperature and humidity sensor; 5-a feed inlet; 6-discharging port; 7-a discharge valve; 8-a scaffold; 101-outer wall of body; 102-an inner wall of the body; 103-a cold water chamber; 201-a water inlet; 202-a hose; 203-rotating cooling pipes; 204-central cooling ring; 205-water passing holes; 206-axis of rotation; 207-a pulley; 208-a driven gear; 209-driving gear; 210-a motor; 211-motor positive and negative rotation automatic controller; 212-a drain opening; 213-an agitator; 301-a sampling tube; 302-a sampling valve; 303-sampling bottle.
Detailed Description
The technical solutions in the embodiments of the present invention will be described in further detail in the following clearly and completely with reference to the accompanying drawings in the embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
As shown in figure 1-2, a liquid fermentation process of soil remediation type microorganism bacterium combination is realized by using a strain combination liquid fermentation device, which comprises a fermentation device body 1, a cooling device 2 and a sampling device 3 which are arranged on the fermentation device body 1, wherein the fermentation device body 1 is provided with a body inner wall 102, a body outer wall 101 and a cold water cavity 103 between the body inner wall 102 and the body outer wall 101, the upper end of the cold water cavity 103 is provided with a water outlet 212 penetrating through the body outer wall 101, the inner wall of a central cooling ring 204 of the cooling device 2 is hermetically connected with the body outer wall 101, the outer wall of the central cooling ring 204 is hermetically connected with the body inner wall 102 to form a sealed space from a rotary cooling pipe 203 to the cold water cavity 103, a rotary shaft 206 connecting a plurality of rotary cooling pipes 203 is arranged in the middle of the inner wall of the central cooling ring 204, and the rotary shaft 206 penetrates through a pulley 207 fixed at the bottom of the fermentation device body 1, the lower end of the rotating shaft 206 is fixed with a driven gear 208, the driven gear 208 is meshed with a driving gear 209 on a motor 210, the motor 210 is electrically connected with a motor forward and reverse rotation automatic controller 211, and the automatic forward rotation and reverse rotation of the motor 210 are controlled to realize the back and forth rotation of the rotary cooling pipe 203 within a certain range.
The rotary cooling pipe 203 is connected with a plurality of stirrers 213, the stirrers 213 are conical cylinders for water storage and buffering, so that the effects of buffering and water storage are achieved, the contact area of cooling water and strain combined liquid is increased, the stirring power is increased, the stirring is more uniform and powerful, the other end of the rotary cooling pipe 203 is hermetically connected with the water inlet 201 through the hose 202, the hose 202 enables the rotary cooling pipe 203 not to influence water inlet when the rotary cooling pipe 203 rotates, meanwhile, the hose 202 arranged at the water inlet does not influence the water inlet and enables the rotary cooling pipe 203 to rotate while the water inlet is filled, the space is saved, the working efficiency of the fermentation device is improved, and external pollution is avoided; a water through hole 205 is formed in the upper end of the rotary cooling pipe 203 and above the central cooling ring 204, the water through hole 205 is in a conical cylinder shape, and the rotary cooling pipe 203 supplies cooling water to the central cooling ring 204 so that the cooling water enters the cold water cavity 103 and is discharged from a water discharge port 212; sampling device 3 is equipped with the sampling bottle 303 of fixing to the 1 outside of fermenting installation body, sampling pipe 301 is connected to sampling bottle 303, and the sampling pipe 301 other end stretches into fermenting installation body 1 in, sampling pipe 301 is equipped with sampling valve 302 in 1 department of fermenting installation body, sampling bottle 303 adopts soft material, and be equipped with the filterable bottle plug of disinfection on the sampling bottle 303, sampling device 3 adopts the siphon principle, sampling bottle 303 adopts soft material, the extrusion sample, make fermenting installation energy-concerving and environment-protective.
The upper part of the fermentation device body 1 is provided with a feeding hole 5, the lower end of the fermentation device body is provided with a discharging hole 6, a discharging valve 7 is arranged on the discharging hole 6, the fermentation device is convenient to clean, and a support 8 is fixed at the bottom of the fermentation device body; a circulating device is adopted between the water inlet 201 and the water outlet 212 of the fermentation device, so that the water is reasonably utilized and the water resource is saved; 1 upper portion of fermenting installation body is equipped with the temperature and humidity sensor 4 who stretches into 1 inboard of fermenting installation body, and temperature and humidity sensor 4 electric connection is located the temperature and humidity sensor 4 display device on 1 upper portion of fermenting installation body, can show the inside operating condition of fermenting installation in real time, is convenient for adjust, makes fermenting installation more stable, accurate.
A liquid fermentation process of a soil remediation type microbial combination is realized by using a soil remediation type microbial combination liquid fermentation device, and comprises the following steps:
1) adding the strain combined liquid into the fermentation device body 1 through a feed port 5 at the upper part of the fermentation device body 1, wherein the adding amount of the strain combined liquid does not exceed the height of the central cooling ring 204;
2) after the addition is finished, the motor 210 and the motor forward and reverse rotation automatic controller 211 are started, the motor forward and reverse rotation automatic controller 211 controls the motor 210 to rotate back and forth between certain angles, the motor 210 drives the rotary cooling pipe 203 to rotate through the rotating shaft 206, and the strain combined liquid is stirred to be uniformly distributed;
3) then cooling water is added through a water inlet 201, the cooling water enters a rotary cooling pipe 203 through a hose 202, the rotary cooling pipe 203 passes through a plurality of conical cylindrical agitators 213 to form storage and buffering, the contact area of the cooling water and the strain combined liquid is increased, when the cooling water passes through the upper part of a central cooling ring 204, the cooling water enters the central cooling ring 204 through a conical cylindrical water passing hole 205, the central cooling ring 204 is communicated with a cold water cavity, and then the cooling water is discharged through a water outlet 212 arranged above the central cooling ring 204 and enters a circulating system;
4) in the whole fermentation process, the environment inside the fermentation device is observed through a display device of the temperature and humidity sensor 4, and corresponding adjustment is made;
5) in the fermentation process, the sampling valve 302 is opened, the soft sampling bottle 303 is pinched by hands, and the strain combined liquid is sucked into the sampling bottle 303 from the fermentation device by utilizing the siphon principle, so that the sample is conveniently extracted and observed, and the fermentation technology is more stable and accurate;
6) after the fermentation is accomplished, open the ejection of compact valve 7 of 1 lower part of fermenting installation body, the bacterial combination liquid that the fermentation was accomplished is collected from discharge gate 6, collects the completion back, can add aseptic clear water from feed inlet 5, washs fermenting installation body 1 through stirring, then from the discharge gate 6 waste water that discharges.
The present invention is not limited to the above embodiments, and any structural changes made under the teaching of the present invention shall fall within the scope of the present invention, which is similar or similar to the technical solutions of the present invention.
The techniques, shapes, and configurations not described in detail in the present invention are all known techniques.

Claims (1)

1. A liquid fermentation process of soil remediation type microbial bacteria combination is characterized by comprising the following steps: comprises that
The fermentation device comprises a fermentation device body and a fermentation carrier of strain combination liquid;
the cooling device is used for providing circulating cooling water for the fermentation device body and cooling the strain combined liquid;
the sampling device is used for sucking strain combined liquid in the fermentation process from the fermentation device body and observing the fermentation degree;
the fermentation device comprises a fermentation device body, a temperature and humidity sensor display device and a temperature and humidity sensor display device, wherein a feeding hole is formed in the upper part of the fermentation device body, a discharging hole is formed in the lower end of the fermentation device body, a discharging valve is arranged on the discharging hole, a support is fixed at the bottom of the fermentation device body, the temperature and humidity sensor extending into the inner side of the fermentation device body is arranged on the upper part of the fermentation device body, and the temperature and humidity sensor display device is electrically connected with the temperature and humidity sensor display device and is used for displaying the working state of the interior of the fermentation device in real time;
the fermentation device body is provided with a body inner wall, a body outer wall and a cold water cavity between the body inner wall and the body outer wall, the upper end of the cold water cavity is provided with a water outlet penetrating through the body outer wall, the inner wall of a central cooling ring of the cooling device is connected with the body outer wall in a sealing way, the outer wall of the central cooling ring is connected with the body inner wall in a sealing way and used for forming a sealed space from a rotary cooling pipe to the cold water cavity, a rotating shaft connected with a plurality of rotary cooling pipes penetrates through the middle of the inner wall of the central cooling ring, the rotating shaft penetrates through a pulley fixed to the bottom of the fermentation device body, a driven gear is fixed at the lower end of the rotating shaft and meshed with a driving gear on a motor, and the motor is electrically connected with a forward and reverse rotation automatic controller of the motor and used for controlling the automatic forward and reverse rotation of the motor;
the rotary cooling pipe is connected with a plurality of stirrers, the stirrers are in a cone cylinder shape with water storage buffering and play roles of buffering and water storage, the contact area of cooling water and strain combined liquid is increased, and meanwhile, the stirring power is increased, so that the stirring is more uniform and powerful;
the sampling device is provided with a sampling bottle fixed to the outer side of the fermentation device body, the sampling bottle is connected with a sampling pipe, the other end of the sampling pipe extends into the fermentation device body, the sampling pipe is provided with a sampling valve at the position of the fermentation device body, the sampling bottle is made of soft materials, the sampling bottle is provided with a bottle plug for disinfection and filtration, the sampling device adopts a siphon principle, and the sampling bottle is made of soft materials and is used for extrusion sampling;
the liquid fermentation process of soil remediation type microbial bacteria combination comprises the following steps:
1) adding the strain combined liquid into the fermentation device body through a feed inlet at the upper part of the fermentation device body, wherein the adding amount of the strain combined liquid does not exceed the height of the central cooling ring;
2) after the addition is finished, the motor and the motor forward and backward rotation automatic controller are turned on, the motor forward and backward rotation automatic controller controls the motor to rotate back and forth, the motor drives the rotary cooling pipe to rotate through the rotating shaft, and the strain combination liquid is stirred to be uniformly distributed;
3) then cooling water is added through a water inlet, the cooling water enters the rotary cooling pipe through the hose, the rotary cooling pipe is provided with a plurality of conical cylindrical agitators to form storage and buffering, the contact area of the cooling water and the strain combined liquid is increased, when the cooling water passes through the upper part of the central cooling ring, the cooling water enters the central cooling ring through the conical cylindrical water passing holes, the central cooling ring is communicated with the cold water cavity, and then the cooling water is discharged through the water outlet at the upper part and enters the circulating system;
4) in the whole fermentation process, the environment inside the fermentation device is observed through a display device of a temperature and humidity sensor, and corresponding adjustment is made;
5) in the fermentation process, a sampling valve is opened, a soft sampling bottle is pinched by hands, and the strain combined liquid is sucked into the sampling bottle from the fermentation device by utilizing the siphon principle, so that the sample is conveniently extracted and observed, and the fermentation technology is more stable and accurate;
6) after the fermentation is completed, a discharging valve at the lower part of the fermentation device body is opened, the fermented strain combined liquid is collected from a discharging port, after the fermentation is completed, sterile clear water is added from a feeding port, the fermentation device body is cleaned by stirring, and then the wastewater is discharged from the discharging port.
CN201910787605.2A 2019-08-26 2019-08-26 Liquid fermentation process of soil remediation type microbial bacterium combination Active CN110358670B (en)

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CA2801768A1 (en) * 2013-01-09 2014-07-09 Soheyl S. M. Mottahedeh A photobioreactor bag with built-in sparger tube, agitator and water
CN103627621B (en) * 2013-12-23 2016-01-20 齐鲁工业大学 Solid-state vertical type fermentation, self-circulation cooling, distillation integrated apparatus and method
CN206512189U (en) * 2017-01-17 2017-09-22 云南羊泉生物科技股份有限公司 Liquid spawn fermentation tank
CN206521464U (en) * 2017-01-24 2017-09-26 江西顺泉生物科技有限公司 A kind of fermentation of bacillus device
CN207445650U (en) * 2017-09-22 2018-06-05 河北天工化工机械制造有限公司 A kind of cooling crystallization machine
CN107471469A (en) * 2017-09-29 2017-12-15 苏州富通高新材料科技股份有限公司 A kind of cold mixing device of section bar dry blend
CN207769643U (en) * 2017-12-22 2018-08-28 山东鹤来生物科技有限公司 A kind of multiple-effect mixed stirring device

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