CN110358064A - A kind of transmitting fluorescent polymer preparation of non-conjugated systems and test method - Google Patents
A kind of transmitting fluorescent polymer preparation of non-conjugated systems and test method Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 238000010998 test method Methods 0.000 title claims abstract description 11
- 229920001109 fluorescent polymer Polymers 0.000 title claims abstract description 9
- JJTUDXZGHPGLLC-UHFFFAOYSA-N lactide Chemical compound CC1OC(=O)C(C)OC1=O JJTUDXZGHPGLLC-UHFFFAOYSA-N 0.000 claims abstract description 25
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 claims abstract description 24
- 230000005284 excitation Effects 0.000 claims abstract description 24
- 239000007787 solid Substances 0.000 claims abstract description 24
- 239000004626 polylactic acid Substances 0.000 claims abstract description 18
- 229920000747 poly(lactic acid) Polymers 0.000 claims abstract description 17
- KSBAEPSJVUENNK-UHFFFAOYSA-L tin(ii) 2-ethylhexanoate Chemical compound [Sn+2].CCCCC(CC)C([O-])=O.CCCCC(CC)C([O-])=O KSBAEPSJVUENNK-UHFFFAOYSA-L 0.000 claims abstract description 15
- 229920000642 polymer Polymers 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 8
- 238000006243 chemical reaction Methods 0.000 claims abstract description 8
- 238000004090 dissolution Methods 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 29
- 238000010790 dilution Methods 0.000 claims description 12
- 239000012895 dilution Substances 0.000 claims description 12
- 230000009467 reduction Effects 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 4
- 229920000728 polyester Polymers 0.000 claims description 3
- 238000011835 investigation Methods 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 150000005846 sugar alcohols Polymers 0.000 claims description 2
- 229920005862 polyol Polymers 0.000 claims 1
- 150000003077 polyols Chemical class 0.000 claims 1
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 abstract description 23
- 238000012360 testing method Methods 0.000 abstract description 11
- 239000000463 material Substances 0.000 abstract description 7
- 239000008103 glucose Substances 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000008859 change Effects 0.000 abstract description 2
- 238000001514 detection method Methods 0.000 abstract description 2
- 239000002086 nanomaterial Substances 0.000 abstract description 2
- 238000001338 self-assembly Methods 0.000 abstract description 2
- 239000000047 product Substances 0.000 description 11
- 238000001291 vacuum drying Methods 0.000 description 4
- 230000002776 aggregation Effects 0.000 description 3
- 238000004220 aggregation Methods 0.000 description 3
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 230000021615 conjugation Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 230000005311 nuclear magnetism Effects 0.000 description 2
- 230000002411 adverse Effects 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 125000005842 heteroatom Chemical group 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 229920000052 poly(p-xylylene) Polymers 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G63/00—Macromolecular compounds obtained by reactions forming a carboxylic ester link in the main chain of the macromolecule
- C08G63/66—Polyesters containing oxygen in the form of ether groups
- C08G63/664—Polyesters containing oxygen in the form of ether groups derived from hydroxy carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G63/00—Macromolecular compounds obtained by reactions forming a carboxylic ester link in the main chain of the macromolecule
- C08G63/78—Preparation processes
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
- C09K2211/14—Macromolecular compounds
- C09K2211/1441—Heterocyclic
- C09K2211/145—Heterocyclic containing oxygen as the only heteroatom
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6419—Excitation at two or more wavelengths
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
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Abstract
The invention discloses a kind of transmitting fluorescent polymer preparation of non-conjugated systems and test methods, weigh DEXTROSE ANHYDROUS, lactide, stannous octoate Sn (Oct)2In flask, toluene dissolution is added, 140 DEG C of points of water flow back 24 hours, instill in anhydrous ether and recrystallize after reaction solution concentration, filter, and obtained solid is dried in vacuo 12 hours, obtains brown solid；The brown solid of preparation is dissolved in DMF solution, polymer solution is configured, and tests fluorescence pattern from low concentration to high concentration.The invention has the benefit that using glucose as polyhydroxy center, a series of different polylactic acid of molecular weight has been synthesized using different feed ratios, test the change in fluorescence under various concentration, with the movement of excitation wavelength, launch wavelength shows regular variation, new test method is provided for the detection of ideal green high molecular material polylactic acid, has especially expanded macromolecular self-assembly medicine-carried system significantly using polylactic acid as the test mode of the Micelle-like Nano-structure of Two of block.
Description
Technical field
The present invention relates to fluorescent polymer preparation technical fields, in particular to a kind of non-conjugated systems to emit fluorescent polymer
Preparation and test method.
Background technique
Luminous organic material applies such as photoelectric device due to unique photophysical property and widely, and chemistry or biology are visited
Needle and bio-imaging field and be concerned.In addition to traditional conjugated organic compounds, there are also some without classical chromophore ratio
Such as the non-traditional luminescent material of aromatic rings.In these materials, segment is flexible and the adjustable non-conjugated polymeric object of structure is because of its tool
There are important basic meaning and wide application prospect and causes the great interest of people.These polymer usually contain electron rich
Hetero atom such as N, O, S etc..Compared with traditional conjugated material, they, which have, prepares simple, environmental-friendly, good biocompatibility etc.
Advantage is very suitable to biomedical applications.
Polylactic acid has superior comprehensive performance as ideal Green Polymer Material, and production process is pollution-free, tool
There are good biocompatibility and biodegradable, terminal catabolite is mainly water and carbon dioxide, intermediate product lactic acid
It is also internal normal sugar metabolism product, there is biological safety.It has been widely used and biological medicine, electronics, daily primary
The every field such as sex aids.
In general, longer excitation and launch wavelength make light be easier to penetrate thicker sample, this facilitates in vivo
Experiment.Meanwhile longer excitation wavelength is smaller to the adverse effect of cell and tissue in biological sample.However, unconventional
Luminescent material is usually in blue and green area transmitting.Although they usually show and excite related luminescence generated by light, several
Without finding effective yellow and red emission.Therefore, synthesis can emit the non-traditional of red spectral band under certain condition
Fluorescent material is most important for exploring Fluorescence Mechanism fluorescent emission rule.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the above technological deficiencies, and it is poly- to provide a kind of non-conjugated systems transmitting fluorescence
Close object preparation and test method.
In order to achieve the above object of the invention, the technical solution adopted by the present invention are as follows: a kind of non-conjugated systems transmitting fluorescence is poly-
Object preparation and test method are closed, centered on polyhydroxy compound glucose, connects polyester based polyalcohol polylactic acid, it is a series of poly-
Close object organic solution have characteristic fluorescence peak, using glucose be polyhydroxy center polylactic acid synthesize, specifically the preparation method is as follows:
Weigh DEXTROSE ANHYDROUS, lactide, stannous octoate Sn (Oct)2In flask, toluene dissolution is added, 140 DEG C of points of water reflux 24 are small
When, it instills in anhydrous ether and recrystallizes after reaction solution concentration, filter, obtained solid is dried in vacuo 12 hours, obtains brown solid；
Fluorometric investigation method is as follows: the brown solid of preparation being dissolved in DMF solution, gradually dilution configures each concentration polymerization
Object solution, and fluorescence pattern, entrance slit 1.75nm, exit slit 1.25nm, excitation are tested from low concentration to high concentration
Wavelength is 380nm, and launch wavelength is 425nm and 485nm.
Further, the DEXTROSE ANHYDROUS, lactide, stannous octoate Sn (Oct)2Feed ratio be (1:70:0.35) or
(1:120:0.6) or (1:180:0.9) or (1:240:1.2) or (1:300:1.5).
Further, fluorescence exciting wavelength is in 380nm, and launch wavelength is in 425nm and 485nm, when fluorescence intensity reaches peak
After value, as concentration increases, fluorescent emission intensity reduces and red shift of wavelength.
Further, excitation wavelength moves to 600nm from 400nm, and launch wavelength moves to 660nm from 500nm, occur red shift and
Fluorescence intensity is reduced with the red shift of excitation wavelength, and fluorescent emission moves to red band by green band.
The invention has the benefit that having been synthesized using different feed ratios a series of using glucose as polyhydroxy center
The different polylactic acid of molecular weight tests the change in fluorescence under various concentration using sepectrophotofluorometer, with excitation wavelength
Mobile, launch wavelength shows regular variation, provides new test for the detection of ideal green high molecular material polylactic acid
Method has especially expanded macromolecular self-assembly medicine-carried system using polylactic acid as the test mode of the Micelle-like Nano-structure of Two of block significantly.
Detailed description of the invention
Fig. 1 is polylactic acid nuclear-magnetism figure；
Fig. 2 is DEXTROSE ANHYDROUS, lactide, 2 feed ratio of Sn (Oct) are 1:70:0.35 products therefrom in λ ex=380nm
Concentration dilution fluorescence pattern；
Fig. 3 is DEXTROSE ANHYDROUS, lactide, Sn (Oct)2Feed ratio is 1:70:0.35 products therefrom in concentration 6mg/mL
Excitation wavelength is incremented by fluorescence pattern；
Fig. 4 is DEXTROSE ANHYDROUS, lactide, 2 feed ratio of Sn (Oct) are 1:120:0.6 products therefrom in λ ex=380nm
Concentration dilution fluorescence pattern；
Fig. 5 is DEXTROSE ANHYDROUS, lactide, Sn (Oct)2Feed ratio is 1:120:0.6 products therefrom in concentration 10mg/mL
Excitation wavelength is incremented by fluorescence pattern.
Fig. 6 is DEXTROSE ANHYDROUS, lactide, 2 feed ratio of Sn (Oct) are 1:180:0.9 products therefrom in λ ex=380nm
Concentration dilution fluorescence pattern；
Fig. 7 is DEXTROSE ANHYDROUS, lactide, Sn (Oct)2Feed ratio is 1:180:0.9 products therefrom in concentration 10mg/mL
Excitation wavelength is incremented by fluorescence pattern；
Fig. 8 is DEXTROSE ANHYDROUS, lactide, 2 feed ratio of Sn (Oct) are 1:240:1.2 products therefrom in λ ex=380nm
Concentration dilution fluorescence pattern；
Fig. 9 is DEXTROSE ANHYDROUS, lactide, Sn (Oct)2Feed ratio is 1:240:1.2 products therefrom in concentration 20mg/mL
Excitation wavelength is incremented by fluorescence pattern.
Figure 10 is DEXTROSE ANHYDROUS, lactide, 2 feed ratio of Sn (Oct) are 1:300:1.5 products therefrom in λ ex=380nm
Concentration dilution fluorescence pattern；
Figure 11 is DEXTROSE ANHYDROUS, lactide, Sn (Oct)2Feed ratio is 1:300:1.5 products therefrom in concentration 20mg/
ML excitation wavelength is incremented by fluorescence pattern.
Specific embodiment
In order that the present invention can be more clearly and readily understood, following will be combined with the drawings in the embodiments of the present invention,
Technical scheme in the embodiment of the invention is clearly and completely described.
Embodiment 1
Fluorescence polylactic acid preparation: DEXTROSE ANHYDROUS, lactide, stannous octoate Sn (Oct) can be emitted2Molar ratio is 1:70:
0.35；DEXTROSE ANHYDROUS, lactide, stannous octoate Sn (Oct) are weighed by above-mentioned molar ratio2In flask, a small amount of toluene is added
Dissolution, 140 DEG C of points of water flow back 24 hours.It instills in anhydrous ether and recrystallizes after reaction solution concentration, filter, obtained solid vacuum is dry
Dry 12 hours, brown solid is obtained, nuclear-magnetism characterization is shown in Fig. 1.
The 0.1g brown solid prepared is dissolved in 5mLDMF solution, concentration 10mg/mL, gradually dilution configures each concentration
Polymer solution, and the test fluorescence pattern from low concentration to high concentration, entrance slit 1.75nm, exit slit 1.25nm,
Excitation wavelength is 380nm, and launch wavelength is 425nm and 485nm, determines that fluorescence intensity maximum concentration is 6mg/mL, in the concentration
Under, increase with excitation wavelength, launch wavelength red shift and fluorescence intensity reduction are specifically shown in Fig. 2, Fig. 3.
Embodiment 2
Fluorescence polylactic acid preparation: DEXTROSE ANHYDROUS, lactide, stannous octoate Sn (Oct) can be emitted2Molar ratio is 1:120:
0.6, DEXTROSE ANHYDROUS, lactide, stannous octoate Sn (Oct) are weighed by above-mentioned molar ratio2In flask, it is molten that a small amount of toluene is added
Solution, 140 DEG C of points of water flow back 24 hours.It instills in anhydrous ether and recrystallizes after reaction solution concentration, filter, obtained solid vacuum drying
12 hours, obtain brown solid.
The 0.3g brown solid prepared is dissolved in 5mLDMF solution, concentration 60mg/mL, gradually dilution configures each concentration
Polymer solution, and the test fluorescence pattern from low concentration to high concentration, entrance slit 1.75nm, exit slit 1.25nm,
Excitation wavelength is 380nm, and launch wavelength is 425nm and 485nm, determines that fluorescence intensity maximum concentration is 10mg/mL, in the concentration
Under, increase with excitation wavelength, launch wavelength red shift and fluorescence intensity reduction are specifically shown in Fig. 4, Fig. 5.
Embodiment 3
Fluorescence polylactic acid preparation: DEXTROSE ANHYDROUS, lactide, stannous octoate Sn (Oct) can be emitted2Molar ratio is 1:180:
0.9, DEXTROSE ANHYDROUS, lactide, stannous octoate Sn (Oct) are weighed by above-mentioned molar ratio2In flask, it is molten that a small amount of toluene is added
Solution, 140 DEG C of points of water flow back 24 hours.It instills in anhydrous ether and recrystallizes after reaction solution concentration, filter, obtained solid vacuum drying
12 hours, obtain brown solid.
The 0.3g brown solid prepared is dissolved in 5mLDMF solution, concentration 60mg/mL, gradually dilution configures each concentration
Polymer solution, and the test fluorescence pattern from low concentration to high concentration, entrance slit 1.75nm, exit slit 1.25nm,
Excitation wavelength is 380nm, and launch wavelength is 425nm and 485nm, determines that fluorescence intensity maximum concentration is 10mg/mL, in the concentration
Under, increase with excitation wavelength, launch wavelength red shift and fluorescence intensity reduction are specifically shown in Fig. 6, Fig. 7.
Embodiment 4
Fluorescence polylactic acid preparation: DEXTROSE ANHYDROUS, lactide, stannous octoate Sn (Oct) can be emitted2Molar ratio is 1:240:
1.2, DEXTROSE ANHYDROUS, lactide, stannous octoate Sn (Oct) are weighed by above-mentioned molar ratio2In flask, it is molten that a small amount of toluene is added
Solution, 140 DEG C of points of water flow back 24 hours.It instills in anhydrous ether and recrystallizes after reaction solution concentration, filter, obtained solid vacuum drying
12 hours, obtain brown solid.
The 0.5g brown solid prepared is dissolved in 5mLDMF solution, concentration 100mg/mL, gradually dilution configuration is each dense
Polymer solution is spent, and tests fluorescence pattern, entrance slit 1.75nm from low concentration to high concentration, exit slit is
1.25nm, excitation wavelength 380nm, launch wavelength are 425nm and 485nm, determine that fluorescence intensity maximum concentration is 20mg/mL,
Under the concentration, increase with excitation wavelength, launch wavelength red shift and fluorescence intensity reduction are specifically shown in Fig. 8, Fig. 9.
Embodiment 5
Fluorescence polylactic acid preparation: DEXTROSE ANHYDROUS, lactide, stannous octoate Sn (Oct) can be emitted2Molar ratio is 1:300:
1.5, DEXTROSE ANHYDROUS, lactide, stannous octoate Sn (Oct) are weighed by above-mentioned molar ratio2In flask, it is molten that a small amount of toluene is added
Solution, 140 DEG C of points of water flow back 24 hours.It instills in anhydrous ether and recrystallizes after reaction solution concentration, filter, obtained solid vacuum drying
12 hours, obtain brown solid.
The 0.5g brown solid prepared is dissolved in 5mLDMF solution, concentration 100mg/mL, gradually dilution configuration is each dense
Polymer solution is spent, and tests fluorescence pattern, entrance slit 1.75nm from low concentration to high concentration, exit slit is
1.25nm, excitation wavelength 380nm, launch wavelength are 425nm and 485nm, determine that fluorescence intensity maximum concentration is 20mg/mL,
Under the concentration, increase with excitation wavelength, launch wavelength red shift and fluorescence intensity reduction are specifically shown in Figure 10, Figure 11.
Mechanism of the present invention is: when polymer is excited in weak solution, emitted energy is consumed by internal molecular motion, is caused glimmering
Luminous intensity is weaker, even without fluorescence.With the increase of concentration, polymer chain is close to each other, and intertwine with each other, and greatly increases
The aggregation of carbonyl.Assemble lone pair electrons and pi-electron interaction and overlapping in cluster, generates spatial conjugation, fluorescence intensity is big
Big enhancing.When concentration further increases, strand is gradually fixed, and carbonyl aggregation cannot effectively occur, and fluorescence intensity is gradually
Weaken.The result is that fluorescence intensity, which presents, first enhances the trend weakened afterwards with the increase of polyester liquid concentration.Since multi-arm is poly-
Lactic acid structure, the generation of spatial conjugation depends on the aggregation between molecular end carbonyl, identical when polymer molecular weight is smaller
Molecular number is more under mass concentration, therefore mass concentration needed for reaching identical fluorescence intensity is smaller.
The present invention and its embodiments have been described above, this description is no restricted, only reality of the invention
One of mode is applied, actual embodiment is not limited thereto.All in all if those skilled in the art are opened by it
Show, without departing from the spirit of the invention, not inventively designs structure side similar with the technical solution
Formula and embodiment, are within the scope of protection of the invention.
Claims (4)
1. a kind of non-conjugated systems transmitting fluorescent polymer preparation and test method, it is characterised in that: with polyol Portugal
Centered on grape sugar, polyester based polyalcohol polylactic acid is connected, a series of organic solution of polymer has characteristic fluorescence peak, with grape
Sugar is that polyhydroxy center polylactic acid synthesizes, it is specific the preparation method is as follows:
Weigh DEXTROSE ANHYDROUS, lactide, stannous octoate Sn (Oct)2In flask, toluene dissolution is added, 140 DEG C of points of water flow back
It 24 hours, instills in anhydrous ether and recrystallizes after reaction solution concentration, filter, obtained solid is dried in vacuo 12 hours, obtains brown
Solid；
Fluorometric investigation method is as follows: the brown solid of preparation being dissolved in DMF solution, gradually it is molten to configure each concentration polymer for dilution
Liquid, and fluorescence pattern, entrance slit 1.75nm, exit slit 1.25nm, excitation wavelength are tested from low concentration to high concentration
For 380nm, launch wavelength is 425nm and 485nm.
2. a kind of non-conjugated systems transmitting fluorescent polymer preparation according to claim 1 and test method, feature exist
In: the DEXTROSE ANHYDROUS, lactide, stannous octoate Sn (Oct)2Feed ratio be (1:70:0.35) or (1:120:0.6) or
(1:180:0.9) or (1:240:1.2) or (1:300:1.5).
3. a kind of non-conjugated systems transmitting fluorescent polymer preparation according to claim 1 and test method, feature exist
In: fluorescence exciting wavelength is in 380nm, and launch wavelength is in 425nm and 485nm, after fluorescence intensity reaches peak value, as concentration increases
Greatly, fluorescent emission intensity reduction and red shift of wavelength.
4. a kind of non-conjugated systems transmitting fluorescent polymer preparation according to claim 1 and test method, feature exist
In: excitation wavelength moves to 600nm from 400nm, and launch wavelength moves to 660nm from 500nm, and red shift occurs and fluorescence intensity is with sharp
It sends out the red shift of wavelength and reduces, fluorescent emission moves to red band by green band.
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Cited By (2)
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| CN111978952A (en) * | 2020-08-26 | 2020-11-24 | 链行走新材料科技(广州)有限公司 | Application of unconjugated fluorescent alternating copolymer in preparation of fluorescent material |
| CN114736363A (en) * | 2022-05-17 | 2022-07-12 | 常州大学 | Fluorescent polymer and method for regulating and controlling luminescent color of fluorescent polymer |
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| US20170000908A1 (en) * | 2013-11-27 | 2017-01-05 | Agency For Science, Technology And Research | A micellar particle |
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| CN111978952A (en) * | 2020-08-26 | 2020-11-24 | 链行走新材料科技(广州)有限公司 | Application of unconjugated fluorescent alternating copolymer in preparation of fluorescent material |
| CN111978952B (en) * | 2020-08-26 | 2023-08-08 | 链行走新材料科技(广州)有限公司 | Application of non-conjugated fluorescent alternating copolymer in preparation of fluorescent material |
| CN114736363A (en) * | 2022-05-17 | 2022-07-12 | 常州大学 | Fluorescent polymer and method for regulating and controlling luminescent color of fluorescent polymer |
| CN114736363B (en) * | 2022-05-17 | 2023-07-25 | 常州大学 | Fluorescent polymers and methods of modulating the luminescent color of fluorescent polymers |
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