CN110357815A - Centipede quinolines and preparation method thereof, purposes - Google Patents

Centipede quinolines and preparation method thereof, purposes Download PDF

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CN110357815A
CN110357815A CN201810312129.4A CN201810312129A CN110357815A CN 110357815 A CN110357815 A CN 110357815A CN 201810312129 A CN201810312129 A CN 201810312129A CN 110357815 A CN110357815 A CN 110357815A
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chromatography
centipede
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CN110357815B (en
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张贵民
刘武占
李艳芳
范建伟
乔建卫
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Lunan Pharmaceutical Group Corp
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/47—Quinolines; Isoquinolines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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Abstract

The present invention relates to a kind of two quinolines noval chemical compounds isolated from centipede and preparation method thereof, purposes, using centipede medicinal material as raw material, pass through extraction, concentration, then through extraction, column chromatography and etc. isolated 3- hydroxyl -8- quinoline sulfuric ester, 3- hydroxyl -4- methoxyl group -8- quinoline sulfuric ester two quinolines noval chemical compounds.Extracorporeal anti-tumor cell activity research has shown that the compounds of this invention has stronger inhibiting effect to selected tumor cell proliferation, has significant anti-tumor activity, can be used for the exploitation of anti-tumor drug and the treatment of clinical tumor.

Description

Centipede quinolines and preparation method thereof, purposes
Technical field
The invention discloses a kind of noval chemical compound isolated from centipede, more particularly to a kind of separated from centipede Two quinolines noval chemical compounds arrived and preparation method thereof, purposes, belong to Chemistry for Chinese Traditional Medicine and field of medicaments.
Background technique
Centipede is China's traditional Chinese medicine, and first recorded in Shennong's Herbal, acrid flavour is warm-natured, toxic, return liver warp, has breath The effect of wind antispastic, removes obstruction in channels to relieve pain, dispersing pathogen accumulation is used for liver wind agitation, and spasm twitch, child convulsion, middle air port belch, half body is not Then, tetanus, rheumatoid arthritis stubborn, migraine and general headache, sore, scrofula, snakebite and bugbite etc..Because there is centipede stronger activating collaterals and eliminating stagnation to attack poison The benefits of, ancient Chinese medicine doctor is also mostly used to treat the old disease persistent ailment such as disease, product, malignant sore.Since modern times, more doctors are treated pernicious swollen with it Tumor obtains good efficacy.
Modern research shows that centipede has the pharmacology such as antitumor, antithrombotic, analgesia, anticoagulant, antibacterial, anticonvulsion, anti-aging Activity, main chemical compositions have protein, polypeptide, polysaccharide, fatty acid, amino acid, microelement and special small molecule chemical combination Object etc..In numerous small molecule compounds, quinolines are various because having antitumor, antibacterial, prevention and cure of cardiovascular disease etc. Pharmacological activity is more concerned by people.
1996, the Jineol that publishes an article, acytotoxic Alkaloid from such as South Korea scholar Surk-Sik Moon The Centipede Scolopendra subspinipes (J.Nat.Prod) is reported for the first time isolates quinoline from Scolopendra subspinipes Quinoline Alkaloid 3,8- dihydroxy quinoline (Jineol), and prove it to human tumour cell line-lung carcinoma cell by test (A-549), ovarian cancer cell (SKOV-3), human colon cancer cell (HCT-15), people's malignant melanoma cell (SK-MEL-2) Deng there is appropriate inhibiting effect.Naoki etc. reports (ANovel Quinoline Alkaloid Possessing a 7- Benzyl Group from the Centipede, Scolopendra subspinipes.Chem.Pharm.Bull.2001) A kind of new quinoline alkaloid 2- hydroxyl -7- [(4- hydroxyl -3- anisyl) methyl] -3- first is isolated from Scolopendra subspinipes It is plain (Scolopendrine) to be named as centipede for oxygroup -8- quinoline sulfate.CN101370781A discloses a kind of for preventing With the novel quinoline class compound for the treatment of cardiovascular disease, which extracted and purify from centipede. CN104529891A discloses 3, the 5- dihydroxy quinoline isolated from Scolopendra subspinipes and its anti-liver cancer and anti-, lung cancer and colon The activity of cancer.
Summary of the invention
The present inventor has made intensive studies centipede, finds two centipede quinolines noval chemical compounds and its antitumor work Property.
The purpose of the present invention is to provide the methods for preparing above-mentioned two centipede quinolines noval chemical compound.
Another object of the present invention is to provide a kind of centipede quinolines with anti-tumor activity and its changes Close object application in preparation of anti-tumor drugs.
Above-mentioned purpose of the invention is achieved by technical solution below, and the technical solution specifically includes two centipedes The extraction of quinolines noval chemical compound separates, Structural Identification, antitumor activity etc..
Have the following structural formula centipede quinolines:
Compound I R1=H
Compound II R1=OCH3。
The preparation step of described two centipede quinolines noval chemical compounds are as follows:
1) it extracts: using centipede as raw material, measuring water, methanol solution or ethanol solution with 6-12 times and extract 1-5 times, filter, close And filtered fluid, it is concentrated into 1 times of amount, obtains extraction concentrate；
2) extract: step 1) extracts ethyl acetate, chloroform, methylene chloride, ether or the acetone extract that concentrate is measured with 1 times 1-6 times, obtain water section；
3) column chromatogram chromatography: the step 2) water section obtains target eluent through column chromatogram chromatography, and concentration obtains concentration mesh Mark eluent；
4) refine: step 3) the concentration target eluent is purified through chromatography, dry, obtains compound I and compound II.
The centipede is Scolopendridae animal Scolopendra subspinipes S.subspinipes mutilans L.Koch, Scolopndra subspinipes multidens (Newport) S.mutidens Newport, blackhead centipede S.negrocapitis Zhang et Wang, Scolopendra subspinipes dehaani The hirudo leech of S.dohaaniBrandt or scolopendra mojiangica S.mojiangica C.Z.Zhang.
1-10BV is first eluted with water by macroporous resin chromatography in the step 2) water section, then with 30%-90% ethyl alcohol 1-6BV is eluted, ethanol eluate is collected, is concentrated into 1 times of amount, through polyamide chromatographic column, 1-10BV is first eluted with water, then use concentration 1-6BV is eluted for 0.001mol/L-0.1mol/L sodium hydroxide solution, collects sodium hydroxide solution eluent, adjusts pH=7.0, It is concentrated into 0.1 times of amount, target eluent must be concentrated.
Step 3) the column chromatogram chromatography is large pore resin absorption column chromatography, polyamide column chromatography chromatography or both connection With.
Preferably, the macroreticular resin be low pole macroreticular resin or in polar macroporous resin, it is preferable that the macropore tree Rouge is one or more of AB-8, D101, XDA-6, XDA-8, DM130, most preferably XDA-8.
Step 4) the chromatography is mesolow chromatography, High-pressure chromatography or both combination, and the chromatograph packing material is anti- Phase silica gel.
Step 3) the concentration target eluent is with the separation of mesolow chromatography eluant, with acetonitrile: 0.1% phosphate aqueous solution= 5-20:95-80 is mobile phase, and Detection wavelength 254nm obtains centipede quinolines I and compound II crude product, described thick Product are with high pressure chromatographic refining, using acetonitrile: 0.1% phosphate aqueous solution=5-20:95-80 is mobile phase, Detection wavelength 254nm, Compound I and compound II, adjust pH=7.0, concentration, concentrate by mesolow chromatography desalination, it is dry to get.
Quinolines new compounds i of the invention and compound ii, by ESI-MS (positive), in conjunction with IR,1H-NMR ,13The analysis of C-NMR map confirms its structure.
Compound I is white powder, and ESI-MS (positive) provides m/z:264.0 [M+Na]+；ESI-MS (negative) m/z:240.0 [M-H] is provided-.By ESI-MS map comprehensive analysis, the molecule of the compound can be determined Amount is 241.0.Because molecular weight is odd number, thus it is speculated that odd number nitrogen may be contained.In conjunction with IR,1H-NMR,13C-NMR determines that its molecular formula is C9H7NO5S。
IR (with pressing potassium bromide troche) map shows 3532.97cm-1, 1573.72cm-1, 1563.59cm-1, 1350.24cm-1, 1319.76cm-1, 1184.73cm-1, information above can speculate containing oxyquinoline structure；Have again 1237.36cm-1, 1046.55cm-1, 759.89cm-1, thus it is speculated that contain SO2。
1H-NMR(DMSO-d6, 400MHz) and display: there are 5 olefinic protons, respectively 8.52 (1H, d, J=of δ in low field area 2.8Hz), δ 7.63 (1H, dd, J=2.0Hz), δ 7.44 (1H, d, J=2.8Hz), δ 7.42 (1H, dd, J=2.0Hz, 8.4Hz), δ 7.39 (1H, t, J=7.2Hz).
13C-NMR(DMSO-d6, 400MHz) and display: 9 carbon signals are shared in low field area, and equal aromatic carbon is composed in conjunction with DEPT It is respectively δ that show 5 methines, which be respectively δ 142.90, δ 127.19, δ 120.73,114.63,4 δ 115.64, δ quaternary carbons, 151.39、δ150.04、δ135.73、δ130.73。
1H-1HCOSY spectrum display H (δ 8.52) is mutually coupled with H (δ 7.44), and H (δ 7.63) and H (δ 7.39) is mutually coupled, H (δ 7.39) it is mutually coupled with H (δ 7.42), H (δ 7.63).
H (δ 8.52) is related to C (δ 142.90) known to hsqc spectrum, and H (δ 7.63) is related to C (δ 114.63), H (δ 7.44) related to C (δ 115.64), H (δ 7.42) is related to C (δ 120.73), and H (δ 7.39) is related to C (δ 127.19).
Composed from HMBC: quaternary carbon C (δ 151.39, C-3) and H (δ 8.52, H-2), H (δ 7.44, H-4) have long-range coupling； C (δ 150.04, C-8) and H (δ 7.63, H-7), H (δ 7.39, H-6) have long-range coupling；C (δ 135.73, C-8a) and H (δ 8.52, H-2), H (δ 7.63, H-7), H (δ 7.44, H-4), H (δ 7.42, H-5) have long-range coupling；C (δ 130.73, C-4a) There is long-range coupling with H (δ 7.42, H-5), H (δ 7.39, H-6).In addition methine carbon C (δ 142.90, C-2) and H (δ 7.44, H- 4) there is long-range coupling；C (δ 120.73, C-5) and H (δ 7.44, H-4) have long-range coupling；C (δ 120.73, C-4) and H (δ 7.42, H-5) there is long-range coupling.In NOESY spectrum: there are NOE signals between H-4 and H-5.Compound I can be confirmed according to the above analysis Molecular structure.It is retrieved, has no duplicate compound therewith, it was demonstrated that the compound is novel compounds, is named as 3- hydroxyl- 8- quinoline sulfuric ester.The NMR data of chemical compounds I is shown in Table 1.
Table 1NMR Data for compound I (in DMSO-d6)
Compound II be pale yellow powder shape solid, ESI-MS (positive) provide m/z:272.0 [M+H]+；Pass through ESI-MS map comprehensive analysis can determine that the molecular weight of the compound is 271.0.Because molecular weight is odd number, thus it is speculated that Ke Nenghan Odd number nitrogen.Determine that its molecular formula is C in conjunction with IR, 1H-NMR, 13C-NMR10H9NO6S。
IR (with pressing potassium bromide troche) map shows 3221.80cm-1,1598.23cm-1,1568.36cm-1, 1344.45cm-1,1313.00cm-1,1174.89cm-1, information above can speculate containing oxyquinoline structure；Have again 1213.00cm-1,1036.05cm-1,702.10cm-1, thus it is speculated that contain SO2；1246.83cm-1,1010.81cm-1, thus it is speculated that Contain C-O-C.
1H-NMR (DMSO-d6,400MHz) display: low field area have 1 active hydrogen be δ 11.02 (1H, br.s, OH) and 4 olefinic protons, respectively δ 8.60 (1H, s), δ 7.88 (1H, d, J=8.8Hz), δ 7.85 (1H, d, J=7.6Hz), δ 7.68 (1H, t, J=8.4Hz)；Having 3 H signals in high field region is δ 4.38 (3H, s, O-CH3).
13C-NMR (DMSO-d6,400MHz) display: 9 carbon signals are shared in low field area, equal aromatic carbon, 1 carbon signal In high field region.Showing 4 methines in conjunction with DEPT spectrum is respectively δ 138.66, δ 128.70, δ 119.65, δ 116.45；1 first Base is δ 62.21；5 quaternary carbon signals are respectively δ 153.87, δ 145.48, δ 141.36, δ 130.34, δ 124.70.
1H-1HCOSY spectrum display H (δ 7.68) is mutually coupled with H (δ 7.88), H (δ 7.85).
H (δ 8.60) is related to C (δ 138.66) known to hsqc spectrum, and H (δ 7.68) is related to C (δ 128.70), H (δ 7.85) related to C (δ 119.65), H (δ 7.88) is related to C (δ 116.45), and H (δ 4.38) is related to C (δ 62.21).
Composed from HMBC: quaternary carbon C (δ 153.87, C-4) and H (δ 8.60, H-2), H (δ 7.88, H-5), H (δ 4.38, OCH3) there is long-range coupling；C (δ 145.48, C-8) and H (δ 7.85, H-7), H (δ 7.68, H-6) have long-range coupling；C(δ 141.36, C-3) there is long-range coupling with H (δ 8.60, H-2)；C (δ 130.34, C-8a) and H (δ 8.60, H-2), H (δ 7.88, H- 5), H (δ 7.85, H-7) has long-range coupling；C (δ 124.70, C-4a) and H (δ 7.88, H-5), H (δ 7.68, H-6) have remotely Coupling.In addition methine carbon C (δ 128.70, C-6) and H (δ 7.88, H-5), H (δ 7.85, H-7) have long-range coupling；C(δ 119.65, C-7) there is long-range coupling with H (δ 7.88, H-5), H (δ 7.68, H-6)；C (δ 116.45, C-5) and H (δ 7.85, H- 7), H (δ 7.68, H-6) has long-range coupling.In NOESY spectrum: there are NOE signals between H-6 and H-5, H-7.Information above can Confirm the structure of compound II.It is retrieved, has no duplicate compound therewith, it was demonstrated that the compound is novel compounds, name For 3- hydroxyl -4- methoxyl group -8- quinoline sulfuric ester.The NMR data of compound ii is shown in Table 2.
Table 2NMR Data for compound II (in DMSO-d6)
It is tested by Compound ira vitro Anti-tumor angiogenesis, further verifying I He of centipede quinolines of the present invention The anti-tumor activity of compound ii, test material, method and result are as follows:
1) test material
Using the action intensity of mtt assay two noval chemical compounds of measurement and to the sensibility of different type tumour cell, with five Fluorouracil (5-Fu, the general pharmaceutical factory in the rising sun East Sea, Shanghai, 25mg/mL, 150511) is used as positive control medicine, and trial drug (is changed Close object I, II) dissolved with culture solution and be diluted to concentration be respectively as follows: 100mg/mL, 50mg/mL, 25mg/mL, 12.5mg/mL, It is 25mg/mL that 6.25mg/mL, 3.125mg/mL and positive drug (5-Fu), which are diluted to concentration with culture medium solution,；Separately set blank Control group.
Cancer cell uses breast cancer cell (MCF-7), lung carcinoma cell (Calu-3), colon cancer cell (HCT-8), liver cancer Cell (HepG2), stomach cancer cell (SGC-7901), cervical cancer cell (Hela), the above cell are all from southern Shandong pharmacy group stock New drug pharmacology centrocyte culturing room, part Co., Ltd.
It is Sigma Products that main agents, which include: RPMI-1640 culture medium, trypsase, MTT, DMSO,；Calf Serum is Hangzhou Sijiqing Biological Engineering Material Co., Ltd.'s product；Remaining reagent is biochemical reagents or analytical grade reagent.
Capital equipment includes: Multiskan FC microplate reader (the silent winged generation that of match), CO2(German Herseus is public for incubator Department).
2) test method
1. inoculating cell: collecting logarithmic phase cell, determine every hole cell number according to the speed of growth of different cells, adjust Concentration of cell suspension, every hole are added 100 μ l edge holes and are filled with sterile PBS, and culture plate is moved into CO after inoculation2Incubator In, cultivate 48h；
2. colour developing: after culture, every hole adds 20 μ l of MTT solution.Continue to cultivate 4h, terminate culture, careful inhale is abandoned in hole Culture supernatant inhales again after needing to be centrifuged for suspension cell and abandons supernatant in hole.Every hole adds 150 μ lDMSO, shakes 10min；
3. colorimetric: under wavelength 570nm (reference 630nm) (blank zeroing), the UV absorption in each hole is measured in microplate reader It is worth (OD), record is as a result, calculate inhibition rate of tumor cell, and calculate IC using SPSS20 software50。
3) test result and conclusion are shown in Table 3.
3 compound I, II Anti-tumor angiogenesis result of table
As seen from the experiment, compound I, II is to the selected breast cancer cell (MCF-7) of test, lung carcinoma cell (Calu- 3), colon cancer cell (HCT-8), liver cancer cells (HepG2), stomach cancer cell (SGC-7901), cervical cancer cell (Hela) etc. are swollen Tumor cell strain all has a strong inhibitory effect, in addition to cervical cancer cell (Hela), to the IC of other tumour cells50In 10 μ g/ ML is hereinafter, show that compound I, II with stronger inhibiting effect, can be used for antitumor the tumor cell proliferation of subjects The exploitation of drug and the prevention and treatment of clinical tumor.
Detailed description of the invention
Attached drawing 1 is the structural formula of compound I, II；
Attached drawing 2 is the HMBC figure of compound I；
Attached drawing 3 is the HMBC map of compound II；
Attached drawing 4 is compound I1H-NMR map；
Attached drawing 5 is compound I13C-NMR map；
Attached drawing 6 is compound II1H-NMR map；
Attached drawing 7 is compound II13C-NMR figure.
Specific embodiment
The present invention is further illustrated by specific embodiment down, but relevant technical staff in the field ought to know, the reality Example is applied not limit the invention in any way.
The preparation of embodiment 1 compound I, II
1) it extracts: using 1.0kg Scolopendra subspinipes as raw material, being extracted 1 time with 6 times of amount water, filtering merges filtered fluid, is concentrated into 1.0kg obtains extraction concentrate；
2) extract: step 1) the extraction concentrate is extracted 1 time with 1.0kg chloroform, obtains water section；
3) column chromatogram chromatography: 1BV is first eluted with water by AB-8 macroporous resin chromatography in the step 2) water section, then With 30% ethanol elution 1BV, ethanol eluate is collected, 1.0kg is concentrated into, through polyamide chromatographic column, 1BV is first eluted with water, then It is 0.001mol/L sodium hydroxide solution elution 1BV with concentration, collects sodium hydroxide solution eluent, adjust pH=7.0, be concentrated into Target eluent must be concentrated in 0.1kg；
4) refine: step 3) the concentration target eluent is with the separation of reverse phase silica gel mesolow chromatography eluant, with acetonitrile: 0.1% phosphate aqueous solution=5:95 is mobile phase, and Detection wavelength 254nm, obtains centipede quinolines I and compound II is thick Product, the crude product is with reverse phase silica gel high pressure chromatographic refining, using acetonitrile: 0.1% phosphate aqueous solution=5:95 detects wave as mobile phase A length of 254nm obtains compound I and compound II, adjusts pH=7.0, concentration, and concentrate is removed by reverse phase silica gel mesolow chromatography Salt, it is dry, obtain compound I 146mg, compound II 43mg.
The preparation of embodiment 2 compound I, II
1) it extracts: using 1.0kg Scolopndra subspinipes multidens (Newport) as raw material, being extracted 4 times with 11 times of 20% ethyl alcohol of amount, filtering merges filtered fluid, It is concentrated into 1.0kg, obtains extraction concentrate；
2) extract: step 1) the extraction concentrate obtains water section with 1.0kg acetone extract 3 times；
3) column chromatogram chromatography: 7BV is first eluted with water by D101 macroporous resin chromatography in the step 2) water section, then With 80% ethanol elution 5BV, ethanol eluate is collected, 1.0kg is concentrated into, through polyamide chromatographic column, 9BV is first eluted with water, then It is 0.08mol/L sodium hydroxide solution elution 5BV with concentration, collects sodium hydroxide solution eluent, adjust pH=7.0, be concentrated into Target eluent must be concentrated in 0.1kg；
4) refine: step 3) the concentration target eluent is with the separation of reverse phase silica gel mesolow chromatography eluant, with acetonitrile: 0.1% phosphate aqueous solution=20:80 is mobile phase, and Detection wavelength 254nm obtains centipede quinolines I and compound II Crude product, the crude product is with reverse phase silica gel high pressure chromatographic refining, using acetonitrile: 0.1% phosphate aqueous solution=20:80 is mobile phase, inspection Survey wavelength is 254nm, obtains compound I and compound II, adjusts pH=7.0, concentration, and concentrate passes through reverse phase silica gel mesolow chromatography Desalination, it is dry, obtain compound I 265mg, compound II 74mg.
The preparation of embodiment 3 compound I, II
1) it extracts: using 1.0kg scolopendra mojiangica as raw material, being extracted 3 times with 9 times of 10% ethyl alcohol of amount, filtering merges filtered fluid, It is concentrated into 1.0kg, obtains extraction concentrate；
2) extract: step 1) the extraction concentrate is extracted 5 times with 1.0kg ethyl acetate, obtains water section；
3) column chromatogram chromatography: 5BV is first eluted with water by DM130 macroporous resin chromatography in the step 2) water section, then With 40% ethanol elution 4BV, ethanol eluate is collected, 1.0kg is concentrated into, through polyamide chromatographic column, 8BV is first eluted with water, then It is 0.05mol/L sodium hydroxide solution elution 6BV with concentration, collects sodium hydroxide solution eluent, adjust pH=7.0, be concentrated into Target eluent must be concentrated in 0.1kg；
4) refine: step 3) the concentration target eluent is with the separation of reverse phase silica gel mesolow chromatography eluant, with acetonitrile: 0.1% phosphate aqueous solution=15:85 is mobile phase, and Detection wavelength 254nm obtains centipede quinolines I and compound II Crude product, the crude product is with reverse phase silica gel high pressure chromatographic refining, using acetonitrile: 0.1% phosphate aqueous solution=15:85 is mobile phase, inspection Survey wavelength is 254nm, obtains compound I and compound II, adjusts pH=7.0, concentration, and concentrate passes through reverse phase silica gel mesolow chromatography Desalination, it is dry, obtain compound I 231mg, compound II 82mg.
The preparation of embodiment 4 compound I, II
1) it extracts: formula centipede being breathed out as raw material using 1.0kg, is extracted 5 times with 8 times of 50% ethyl alcohol of amount, filtering merges filtered fluid, It is concentrated into 1.0kg, obtains extraction concentrate；
2) extract: step 1) the extraction concentrate is extracted 4 times with 1.0kg ether, obtains water section；
3) column chromatogram chromatography: 3BV is first eluted with water by XDA-8 macroporous resin chromatography in the step 2) water section, then With 20% ethanol elution 6BV, ethanol eluate is collected, 1.0kg is concentrated into, through polyamide chromatographic column, 2BV is first eluted with water, then It is 0.01mol/L sodium hydroxide solution elution 2BV with concentration, collects sodium hydroxide solution eluent, adjust pH=7.0, be concentrated into Target eluent must be concentrated in 0.1kg；
4) refine: step 3) the concentration target eluent is with the separation of reverse phase silica gel mesolow chromatography eluant, with acetonitrile: 0.1% phosphate aqueous solution=11:89 is mobile phase, and Detection wavelength 254nm obtains centipede quinolines I and compound II Crude product, the crude product is with reverse phase silica gel high pressure chromatographic refining, using acetonitrile: 0.1% phosphate aqueous solution=11:89 is mobile phase, inspection Survey wavelength is 254nm, obtains compound I and compound II, adjusts pH=7.0, concentration, and concentrate passes through reverse phase silica gel mesolow chromatography Desalination, it is dry, obtain compound I 193mg, compound II 51mg.
The preparation of embodiment 5 compound I, II
1) it extracts: using 1.0kg Scolopendra subspinipes as raw material, being extracted 2 times with 10 times of 70% ethyl alcohol of amount, filtering merges filtered fluid, It is concentrated into 1.0kg, obtains extraction concentrate；
2) extract: step 1) the extraction concentrate is extracted 4 times with 1.0kg ethyl acetate, obtains water section；
3) column chromatogram chromatography: 6BV is first eluted with water by AB-8 macroporous resin chromatography in the step 2) water section, then With 50% ethanol elution 3BV, ethanol eluate is collected, 1.0kg is concentrated into, through polyamide chromatographic column, 6BV is first eluted with water, then It is 0.005mol/L sodium hydroxide solution elution 3BV with concentration, collects sodium hydroxide solution eluent, adjust pH=7.0, be concentrated into Target eluent must be concentrated in 0.1kg；
4) refine: step 3) the concentration target eluent is with the separation of reverse phase silica gel mesolow chromatography eluant, with acetonitrile: 0.1% phosphate aqueous solution=20:80 is mobile phase, and Detection wavelength 254nm obtains centipede quinolines I and compound II Crude product, the crude product is with reverse phase silica gel high pressure chromatographic refining, using acetonitrile: 0.1% phosphate aqueous solution=20:80 is mobile phase, inspection Survey wavelength is 254nm, obtains compound I and compound II, adjusts pH=7.0, concentration, and concentrate passes through reverse phase silica gel mesolow chromatography Desalination, it is dry, obtain compound I 209mg, compound II 84mg.
The preparation of embodiment 6 compound I, II
1) it extracts: using 1.0kg blackhead centipede as raw material, being extracted 4 times with 7 times of amount water, filtering merges filtered fluid, is concentrated into 1.0kg obtains extraction concentrate；
2) extract: step 1) the extraction concentrate is extracted 2 times with 1.0kg methylene chloride, obtains water section；
3) column chromatogram chromatography: 10BV is first eluted with water by D101 macroporous resin chromatography in the step 2) water section, then With 50% ethanol elution 2BV, ethanol eluate is collected, 1.0kg is concentrated into, through polyamide chromatographic column, 3BV is first eluted with water, then It is 0.09mol/L sodium hydroxide solution elution 3BV with concentration, collects sodium hydroxide solution eluent, adjust pH=7.0, be concentrated into Target eluent must be concentrated in 0.1kg；
4) refine: step 3) the concentration target eluent is with the separation of reverse phase silica gel mesolow chromatography eluant, with acetonitrile: 0.1% phosphate aqueous solution=16:84 is mobile phase, and Detection wavelength 254nm obtains centipede quinolines I and compound II Crude product, the crude product is with reverse phase silica gel high pressure chromatographic refining, using acetonitrile: 0.1% phosphate aqueous solution=16:84 is mobile phase, inspection Survey wavelength is 254nm, obtains compound I and compound II, adjusts pH=7.0, concentration, and concentrate passes through reverse phase silica gel mesolow chromatography Desalination, it is dry, obtain compound I 214mg, compound II 73mg.
The preparation of embodiment 7 compound I, II
1) it extracts: using 1.0kg Scolopndra subspinipes multidens (Newport) as raw material, being extracted 3 times with 10 times of 60% ethyl alcohol of amount, filtering merges filtered fluid, It is concentrated into 1.0kg, obtains extraction concentrate；
2) extract: step 1) the extraction concentrate is extracted 1 time with 1.0kg ether, obtains water section；
3) column chromatogram chromatography: 2BV is first eluted with water by DM130 macroporous resin chromatography in the step 2) water section, then With 80% ethanol elution 4BV, ethanol eluate is collected, 1.0kg is concentrated into, through polyamide chromatographic column, 2BV is first eluted with water, then It is 0.003mol/L sodium hydroxide solution elution 6BV with concentration, collects sodium hydroxide solution eluent, adjust pH=7.0, be concentrated into Target eluent must be concentrated in 0.1kg；
4) refine: step 3) the concentration target eluent is with the separation of reverse phase silica gel mesolow chromatography eluant, with acetonitrile: 0.1% phosphate aqueous solution=8:92 is mobile phase, and Detection wavelength 254nm, obtains centipede quinolines I and compound II is thick Product, the crude product is with reverse phase silica gel high pressure chromatographic refining, using acetonitrile: 0.1% phosphate aqueous solution=8:92 detects wave as mobile phase A length of 254nm obtains compound I and compound II, adjusts pH=7.0, concentration, and concentrate is removed by reverse phase silica gel mesolow chromatography Salt, it is dry, obtain compound I 230mg, compound II 81mg.
The preparation of embodiment 8 compound I, II
1) it extracts: using 1.0kg Scolopndra subspinipes multidens (Newport) as raw material, being extracted 3 times with 8 times of 50% ethyl alcohol of amount, filtering merges filtered fluid, It is concentrated into 1.0kg, obtains extraction concentrate；
2) extract: step 1) the extraction concentrate is extracted 3 times with 1.0kg ethyl acetate, obtains water section；
3) column chromatogram chromatography: 5BV is first eluted with water by XDA-8 macroporous resin chromatography in the step 2) water section, then With 50% ethanol elution 3BV, ethanol eluate is collected, 1.0kg is concentrated into, through polyamide chromatographic column, 5BV is first eluted with water, then It is 0.01mol/L sodium hydroxide solution elution 3BV with concentration, collects sodium hydroxide solution eluent, adjust pH=7.0, be concentrated into Target eluent must be concentrated in 0.1kg；
4) refine: step 3) the concentration target eluent is with the separation of reverse phase silica gel mesolow chromatography eluant, with acetonitrile: 0.1% phosphate aqueous solution=10:90 is mobile phase, and Detection wavelength 254nm obtains centipede quinolines I and compound II Crude product, the crude product is with reverse phase silica gel high pressure chromatographic refining, using acetonitrile: 0.1% phosphate aqueous solution=10:90 is mobile phase, inspection Survey wavelength is 254nm, obtains compound I and compound II, adjusts pH=7.0, concentration, and concentrate passes through reverse phase silica gel mesolow chromatography Desalination, it is dry, obtain compound I 297mg, compound II 86mg.
The preparation of embodiment 9 compound I, II
1) it extracts: using 1.0kg Scolopendra subspinipes as raw material, being extracted 1 time with 10 times of 30% ethyl alcohol of amount, filtering merges filtered fluid, It is concentrated into 1.0kg, obtains extraction concentrate；
2) extract: step 1) the extraction concentrate is extracted 6 times with 1.0kg ethyl acetate, obtains water section；
3) column chromatogram chromatography: 9BV is first eluted with water by XDA-6 macroporous resin chromatography in the step 2) water section, then With 90% ethanol elution 6BV, ethanol eluate is collected, 1.0kg is concentrated into, through polyamide chromatographic column, 4BV is first eluted with water, then It is 0.1mol/L sodium hydroxide solution elution 5BV with concentration, collects sodium hydroxide solution eluent, adjust pH=7.0, be concentrated into Target eluent must be concentrated in 0.1kg；
4) refine: step 3) the concentration target eluent is with the separation of reverse phase silica gel mesolow chromatography eluant, with acetonitrile: 0.1% phosphate aqueous solution=12:8 is mobile phase, and Detection wavelength 254nm, obtains centipede quinolines I and compound II is thick Product, the crude product is with reverse phase silica gel high pressure chromatographic refining, using acetonitrile: 0.1% phosphate aqueous solution=12:8 detects wave as mobile phase A length of 254nm obtains compound I and compound II, adjusts pH=7.0, concentration, and concentrate is removed by reverse phase silica gel mesolow chromatography Salt, it is dry, obtain compound I 202mg, compound II 67mg.
The preparation of embodiment 10 compound I, II
1) it extracts: using 1.0kg Scolopendra subspinipes dehaani as raw material, being extracted 2 times with 9 times of amount water, filtering merges filtered fluid, is concentrated into 1.0kg obtains extraction concentrate；
2) extract: step 1) the extraction concentrate obtains water section with 1.0kg acetone extract 5 times；
3) column chromatogram chromatography: 7BV is first eluted with water by AB-8 macroporous resin chromatography in the step 2) water section, then With 60% ethanol elution 2BV, ethanol eluate is collected, 1.0kg is concentrated into, through polyamide chromatographic column, 8BV is first eluted with water, then It is 0.08mol/L sodium hydroxide solution elution 4BV with concentration, collects sodium hydroxide solution eluent, adjust pH=7.0, be concentrated into Target eluent must be concentrated in 0.1kg；
4) refine: step 3) the concentration target eluent is with the separation of reverse phase silica gel mesolow chromatography eluant, with acetonitrile: 0.1% phosphate aqueous solution=17:83 is mobile phase, and Detection wavelength 254nm obtains centipede quinolines I and compound II Crude product, the crude product is with reverse phase silica gel high pressure chromatographic refining, using acetonitrile: 0.1% phosphate aqueous solution=17:83 is mobile phase, inspection Survey wavelength is 254nm, obtains compound I and compound II, adjusts pH=7.0, concentration, and concentrate passes through reverse phase silica gel mesolow chromatography Desalination, it is dry, obtain compound I 198mg, compound II 54mg.
The preparation of embodiment 11 compound I, II
1) it extracts: using 1.0kg blackhead centipede as raw material, being extracted 3 times with 12 times of 40% methanol of amount, filtering merges filtered fluid, It is concentrated into 1.0kg, obtains extraction concentrate；
2) extract: step 1) the extraction concentrate is extracted 6 times with 1.0kg ethyl acetate, obtains water section；
3) column chromatogram chromatography: 6BV is first eluted with water by XDA-8 macroporous resin chromatography in the step 2) water section, then With 80% ethanol elution 1BV, ethanol eluate is collected, 1.0kg is concentrated into, through polyamide chromatographic column, 10BV is first eluted with water, then It is 0.04mol/L sodium hydroxide solution elution 1BV with concentration, collects sodium hydroxide solution eluent, adjust pH=7.0, be concentrated into Target eluent must be concentrated in 0.1kg；
4) refine: step 3) the concentration target eluent is with the separation of reverse phase silica gel mesolow chromatography eluant, with acetonitrile: 0.1% phosphate aqueous solution=9:91 is mobile phase, and Detection wavelength 254nm, obtains centipede quinolines I and compound II is thick Product, the crude product is with reverse phase silica gel high pressure chromatographic refining, using acetonitrile: 0.1% phosphate aqueous solution=9:91 detects wave as mobile phase A length of 254nm obtains compound I and compound II, adjusts pH=7.0, concentration, and concentrate is removed by reverse phase silica gel mesolow chromatography Salt, it is dry, obtain compound I 263mg, compound II 61mg.
The preparation of embodiment 12 compound I, II
1) it extracts: using 1.0kg Scolopndra subspinipes multidens (Newport) as raw material, being extracted 5 times with 6 times of 80% methanol of amount, filtering merges filtered fluid, It is concentrated into 1.0kg, obtains extraction concentrate；
2) extract: step 1) the extraction concentrate is extracted 3 times with 1.0kg ethyl acetate, obtains water section；
3) column chromatogram chromatography: 4BV is first eluted with water by DM130 macroporous resin chromatography in the step 2) water section, then With 70% ethanol elution 5BV, ethanol eluate is collected, 1.0kg is concentrated into, through polyamide chromatographic column, 7BV is first eluted with water, then It is 0.09mol/L sodium hydroxide solution elution 2BV with concentration, collects sodium hydroxide solution eluent, adjust pH=7.0, be concentrated into Target eluent must be concentrated in 0.1kg；
4) refine: step 3) the concentration target eluent is with the separation of reverse phase silica gel mesolow chromatography eluant, with acetonitrile: 0.1% phosphate aqueous solution=15:85 is mobile phase, and Detection wavelength 254nm obtains centipede quinolines I and compound II Crude product, the crude product is with reverse phase silica gel high pressure chromatographic refining, using acetonitrile: 0.1% phosphate aqueous solution=15:85 is mobile phase, inspection Survey wavelength is 254nm, obtains compound I and compound II, adjusts pH=7.0, concentration, and concentrate passes through reverse phase silica gel mesolow chromatography Desalination, it is dry, obtain compound I 270mg, compound II 58mg.

Claims (10)

1. having the following structural formula centipede quinolines:
Compound I R1=H
Compound II R1=OCH3。
2. the preparation method of centipede quinolines as described in claim 1, which comprises the following steps:
1) it extracts: using centipede as raw material, measuring water, methanol solution or ethanol solution with 6-12 times and extract 1-5 times, filtering merged Filtrate is concentrated into 1 times of amount, obtains extraction concentrate；
2) extract: step 1) extracts ethyl acetate, chloroform, methylene chloride, ether or the acetone extract 1-6 that concentrate is measured with 1 times It is secondary, obtain water section；
3) column chromatogram chromatography: the step 2) water section obtains target eluent through column chromatogram chromatography, and concentration must be concentrated target and wash De- liquid；
4) refine: step 3) the concentration target eluent is purified through chromatography, dry, obtains compound I and compound II.
3. preparation method as claimed in claim 2, which is characterized in that the step 1) centipede is that Scolopendridae animal lacks spine Wu Centipede, Scolopndra subspinipes multidens (Newport), blackhead centipede, Scolopendra subspinipes dehaani or scolopendra mojiangica hirudo leech.
4. preparation method as claimed in claim 2, which is characterized in that the step 3) column chromatogram chromatography is macroporous absorbent resin Column chromatogram chromatography, polyamide column chromatography chromatography or both combination.
5. preparation method as claimed in claim 2, which is characterized in that the step 2) water section passes through macroreticular resin chromatography Column, is first eluted with water 1-10BV, then with 30%-90% ethanol elution 1-6BV, collects ethanol eluate, be concentrated into 1 times of amount, warp Polyamide chromatographic column, is first eluted with water 1-10BV, then with concentration is 0.001mol/L-0.1mol/L sodium hydroxide solution elution 1- 6BV collects sodium hydroxide solution eluent, adjusts pH=7.0, is concentrated into 0.1 times of amount, target eluent must be concentrated.
6. preparation method as claimed in claim 2, which is characterized in that step 4) the concentration target eluent is with mesolow color Spectrum elution separation, using acetonitrile: for 0.1% phosphate aqueous solution=5-20:95-80 as mobile phase, Detection wavelength 254nm obtains centipede Quinolines I and compound II crude product, the crude product is with high pressure chromatographic refining, with acetonitrile: 0.1% phosphate aqueous solution=5- 20:95-80 is mobile phase, and Detection wavelength 254nm obtains compound I and compound II, adjusts pH=7.0, concentration, and concentrate leads to Cross mesolow chromatography desalination, it is dry to get.
7. the preparation method as described in any one of claim 2-6, which is characterized in that the macroreticular resin is low pole macropore Resin or in polar macroporous resin, it is preferred that described macroreticular resin model AB-8, D101, XDA-6, XDA-8 or DM130, Optimal is XDA-8.
8. the preparation method as described in any one of claim 2-6, which is characterized in that the step 4) chromatography is mesolow Chromatography, High-pressure chromatography or both combination, it is preferred that the chromatograph packing material is reverse phase silica gel.
9. the purposes of centipede quinolines as described in claim 1 in the preparation of antitumor drugs.
10. purposes as claimed in claim 9, which is characterized in that the tumour is breast cancer, lung cancer, liver cancer, gastric cancer, colon Cancer and cervical carcinoma.
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