CN110346441A - A kind of grapheme modified field effect transistor antibiotic sensor of aptamers - Google Patents
A kind of grapheme modified field effect transistor antibiotic sensor of aptamers Download PDFInfo
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- CN110346441A CN110346441A CN201910578201.2A CN201910578201A CN110346441A CN 110346441 A CN110346441 A CN 110346441A CN 201910578201 A CN201910578201 A CN 201910578201A CN 110346441 A CN110346441 A CN 110346441A
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Abstract
The present invention relates to a kind of grapheme modified field effect transistor antibiotic sensors of aptamers, including grid (1), source electrode (3) and drain electrode (4), grid (1) surface is covered with single side oxidized silicon chip as electrode base sheet (2), the oxide layer area load of the electrode base sheet (2) has graphene layer (5), graphene layer (5) the surface deposition probe connector (6), aptamers detection probe (7) are connected on the probe connector (6), by by source electrode (3), it drains (4) and grid (1) access semiconductor analysis instrument detects response signal.Compared with prior art, the present invention has excellent detectability to tobramycin in water, extremely low detection limit, capability of fast response and outstanding selectivity, chemical stability with higher and stable signal fan-out capability are able to achieve the detection of antibiotic in current water with PDMS microchannel integrated sensor micro flow chip.
Description
Technical field
The present invention relates to a kind of chemical biosensors, more particularly, to a kind of grapheme modified field effect transistor of aptamers
Pipe antibiotic sensor.
Background technique
Since agricultural, animal husbandry, aquaculture and medical industry are to excessively using and discharging to antibiotic, result in anti-
Raw element remains in the environment, including water body environment.As novel water pollutant, antibiotic residue is to ecological environment and people in water
Body health all has serious threat.Concern with people to water quality and public safety is proposed to water body antibiotic content
The new demand of detection.Since traditional antibiotic detection mode is based primarily upon the analysis hand of the instrument including liquid and gas chromatography
Section, it is complicated for operation and it is time-consuming long, thus need original position, real-time detection means provide instant antibiotic levels information, with carry out
Big data analysis and pollution prewarning.
Chinese patent CN201410114665.5 discloses a kind of preparation of aptamer sensor for detecting antibiotic residue
Method belongs to agricultural product security detection technique field.The present invention obtains electropolymerization by the way that gold electrode and thionine are carried out electropolymerization
Thionine electrode, then the pre-assigned uniform drop coating of carbon nanotube-graphene nanometer composite is dried in the air to electropolymerization thionine electrode surface
After dry, then electrode is immersed in antibiotic and is adapted in liquid solution, rinsed after drying, then closed with BSA solution, it is suitable to obtain antibiotic
With body sensor.The patent is electrochemical sensor, and nano-complex, aptamers, BSA confining bed are that physics is negative in sensor
It carries, structural stability is poor.
FET sensor is a kind of semi-conductor electricity resistance type sensor, is based on FET device working mechanism, by logical
Road material semiconductor performance change in electrical signal form makes a response test object.Receiving including graphene in recent years
While the research of rice Material Field obtains continuous development with breaking through, the performance of this sensor obtains progressive promotion, thus
Attract wide public concern and in-depth study, be it is a kind of extremely have development potentiality suitable for the sensor type that quickly detects.
Selection and structure design by way of channel material, detection probe and modification can flexibly be used for the detection of different objects.It is this kind of
Sensor has highly sensitive (extremely low Monitoring lower-cut), quick response (response time is in the order of seconds), can carry out in real time
The advantages that monitoring, minimize, can integrate, is inexpensive.
Summary of the invention
It is an object of the present invention to overcome the above-mentioned drawbacks of the prior art and provide a kind of aptamers to modify stone
Black alkene field effect transistor antibiotic sensor.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of grapheme modified field effect transistor antibiotic sensor of aptamers, including grid, source electrode and drain electrode, it is described
Gate surface is covered with single side oxidized silicon chip as electrode base sheet, and the electrode base sheet area load has the stone of bridging source electrode and drain electrode
Black alkene layer, the graphene layer surface deposition probe connector are connected with aptamers detection probe on the probe connector, lead to
It crosses source electrode, drain and gate access semiconductor analysis instrument detection response signal.The sensor is inserted with adjacent gold refers to that electrode is
The source electrode and drain electrode of field effect transistor, the silicon chip back side are that grid accesses the progress electric signal analysis of external semiconductor analyzer.
Further, the probe connector is the nanogold particle being deposited on graphene layer.
Further, the aptamers detection probe is aptamers RNA antibiotic probe.
Further, the aptamers RNA antibiotic probe chemical structure and nucleotides sequence are classified as HSC6-5 '-
GGCACGAGGUUUAGCUACACUCGUGCC-3’。
Further, the aptamers detection probe is modified by way of hatching 12h in the solution in probe connector
Surface is successfully modified with golden sulfide linkage in nanometer gold surface.
Further, the solution is the PBS buffer solution containing three (2- carboxyethyl) phosphine disulfide bond activators, adaptation
Bulk concentration is 20 μM.
Further, the oxidated layer thickness of the electrode base sheet is 300nm.
Further, the source electrode and drain electrode is that gold inserts finger electrode, and width and gap are respectively 2 μm and 1.5 μm.
Further, the graphene layer refers to that the graphene oxide of electrode is annealed in argon gas high temperature by being deposited on gold and inserting
After obtain, annealing temperature be 400 DEG C, annealing time 1h.
Further, graphene oxide is spontaneously dried by 0.001mg/mL stannic oxide/graphene nano piece aqueous dispersion liquid
It arrives.
Further, the probe connector of receiving on the graphene layer surface is existed by sputter coating technical controlling sputtering time
2s deposits to be formed.
Further, the probe connector exposed part is provided with 6- sulfydryl hex- 1- alcohol confining bed, improves device chemistry
Stability and stable signal fan-out capability.
Further, the part of the graphene layer surface exposure is provided with 1- pyrene butanol confining bed, improves device chemistry
Stability and stable signal fan-out capability.
Further, 6- sulfydryl hex- 1- alcohol is hatched after the chemical confining bed modification sequence is 1- pyrene butanol hatching 6h
12h。
Further, the sensor and PDMS microchannel integrate microfluidic sensing chip, realize current water
The detection of middle antibiotic.
Further, the PDMS microchannel is 600 μm wide, 200 μm deep.
Further, the PDMS microchannel and transistor sensor substrate are integrated by oxygen plasma bonding method.
Further, for when antibiotic detection, water body example flow regime to pass through external micro note in current water
Pump control is penetrated, flow velocity is 50 μ L/min.
The grapheme modified field effect transistor antibiotic of aptamers senses implement body manufacturing process are as follows: refers to electrode zone inserting
It is added dropwise graphene oxide dispersion liquid (1 μ L, 0.001mg/mL), natural drying forms graphene oxide layer.Place it in argon gas
In atmosphere, with 400 DEG C of annealing 1h, redox graphene two-dimensional nano channel is obtained.After being cooled to room temperature, it is placed in vacuum
Plated film instrument cavity is that target as sputter 2s deposits gold nano grain with gold.It is mould that appropriate cloth is dispersed with the PBS buffer solution containing TEPC simultaneously
Plain aptamers RNA forms 20 μM of adaptation dispersion liquid, and covering graphene/nanometer gold region is added dropwise, and keeps wet and stands hatching
12h.After deionized water repeated flushing washes away unmodified aptamers and impurity, the dichloromethane solution of 1- pyrene butanol is added dropwise, after 6h
It is dried up with ethyl alcohol and deionized water repeated flushing and with argon gas, exposed graphene layer surface is closed with π-π effect；Then 6- is added dropwise
It ethyl alcohol and deionized water repeated flushing and is dried up after sulfydryl hex- 1- alcohol hatching 12h with argon gas, it is exposed with the modification closing of golden sulfide linkage
Nanometer gold surface.Sensor chip after oxygen plasma is surface-treated is bonded to obtain microfluidic sensing core with PDMS microchannel
Piece measures the appropriate cloth in current water with flow regime of the external micro-injection pump control solution example in microchannel simultaneously
Mycin content.
The course of work of sensor are as follows: the bias (0.1V) for applying a very little between source electrode and drain electrode passes through external circuit
Real-time detection the current between the source and the drain, to export the variation characterization signal response of electric current.When containing tobramycin to
It surveys solution to be added dropwise in inserting finger electrode zone, tobramycin molecule makes RNA chain form folding configuration, RNA in conjunction with aptamers RNA
Negative electrical charge on chain changes at a distance from channel material, causes the semiconductor of channel material graphene special by electrostatic interaction
Property, p-type doping occurs, surface voids concentration increases；Semiconductor analysis instrument is dense with antibiotic in solution example to be measured by monitoring
Relevant source and drain interpolar curent change is spent, so that it is determined that the concentration of the tobramycin in solution example.
Compared with prior art, the beneficial effects of the present invention are:
1. the present invention is low to the detection limit of tobramycin in water, selectivity is high, and response is fast, it can be achieved that quickly detection；
2. device chemistry of the present invention and electrical stability are good, there is stable signal fan-out capability, it is at low cost, it can integrate；
3. the present invention is able to achieve the inspection of the antibiotic in current water with PDMS microchannel integrated sensor micro flow chip
It surveys.
Detailed description of the invention
The structural schematic diagram of Fig. 1 inventive sensor；
Fig. 2 is response signal figure of the present invention to various concentration tobramycin water sample；
Fig. 3 be the present invention to the response signal figure of Multiple Classes of Antibiotics water sample (be respectively in order kanamycins, streptomysin,
Ciprofloxacin, tetracycline, tobramycin, concentration are 10-5M)；
Fig. 4 is flowing water sample response signal of the integrated microfluidic sensing chip of the present invention to various concentration tobramycin
Figure.
Shown in figure label:
1, grid, 2, electrode base sheet, 3, source electrode, 4, drain electrode, 5, graphene layer, 6, probe connector, 7, aptamers detection
Probe, 8,1- pyrene butanol confining bed, 9,6- sulfydryl -1- alcohol confining bed.
Specific embodiment
The present invention is described in detail with specific embodiment below in conjunction with the accompanying drawings.
Embodiment 1
Referring to Fig.1 shown in, a kind of grapheme modified field effect transistor antibiotic sensor of aptamers, including grid 1 with
And the silicon dioxide layer in silicon base is as electrode base sheet 2, to be lithographically formed the source electrode 3 for inserting and referring to and being interspersed in silicon dioxide layer
It is formed to insert with drain electrode 4 and refers to electrode zone, deposition graphene layer 5 is used as channel material between source electrode 3 and drain electrode 4, in graphene layer 5
Nanogold particle is deposited as probe connector 6, tobramycin aptamers is modified on nanogold particle and is detected as aptamers
Probe 7, to the nanometer gold surface of the graphene surface and unmodified aptamers that do not deposit nanogold modify respectively 1- pyrene butanol and
6- sulfydryl hex- 1- alcohol forms 1- pyrene butanol confining bed 8 and 6- sulfydryl hex- 1- alcohol confining bed 9, by silicone based crystal tube sensor with
PDMS microchannel is integrated into microfluidic sensing chip for realizing the detection of tobramycin in current water.
The grapheme modified field effect transistor antibiotic of aptamers senses implement body manufacturing process are as follows: refers to electrode zone inserting
It is added dropwise graphene oxide dispersion liquid (1 μ L, 0.001mg/mL), natural drying forms graphene oxide layer 5.Place it in argon gas
In atmosphere, with 400 DEG C of annealing 1h, redox graphene two-dimensional nano channel is obtained.After being cooled to room temperature, it is placed in vacuum
Plated film instrument cavity is that target as sputter 2s deposits gold nano grain with gold.It is mould that appropriate cloth is dispersed with the PBS buffer solution containing TEPC simultaneously
Plain aptamers RNA forms 20 μM of adaptation dispersion liquid, and covering graphene/nanometer gold region is added dropwise, and keeps wet and stands hatching
12h.After deionized water repeated flushing washes away unmodified aptamers and impurity, the dichloromethane solution of 1- pyrene butanol is added dropwise, after 6h
It is dried up with ethyl alcohol and deionized water repeated flushing and with argon gas, exposed 5 surface of graphene layer is closed with π-π effect；Then it is added dropwise
It ethyl alcohol and deionized water repeated flushing and is dried up after 6- sulfydryl hex- 1- alcohol hatching 12h with argon gas, it is exposed with the modification closing of golden sulfide linkage
Nanometer gold surface.Sensor chip after oxygen plasma is surface-treated is bonded to obtain microfluidic sensing with PDMS microchannel
Chip is measured appropriate in current water simultaneously with flow regime of the external micro-injection pump control solution example in microchannel
Obramycin content.
The course of work of sensor are as follows: the bias (0.1V) for applying a very little between source electrode 3 and drain electrode 4 passes through dispatch from foreign news agency
Electric current between road real-time detection source electrode 3 and drain electrode 4, to export the variation characterization signal response of electric current.When containing tobramycin
Solution to be measured be added dropwise in insert refer to electrode zone, tobramycin molecule with aptamers RNA in conjunction with, make RNA chain formation folding configuration,
Negative electrical charge on RNA chain changes at a distance from channel material, causes partly leading for channel material graphene by electrostatic interaction
Bulk properties, occurs p-type doping, and surface voids concentration increases；Semiconductor analysis instrument passes through monitoring and antibiosis in solution example to be measured
Concentration dependent 4 curent changes of source-drain electrode of element, so that it is determined that the concentration of the tobramycin in solution example.
A certain amount of 10 are prepared respectively-8M、10-7M、10-6M、10-5M、10-4M tobramycin solution；The sensor that will be prepared
Source electrode 3, drain electrode 4 and grid 1 respectively correspond in access semiconductor analysis instrument, and setting grid 1 is grounded, between source electrode 3 and drain electrode 4
The constant voltage of application+0.1V；It is successively inserted in sensor and refers to that (1 μ L) is added dropwise between electrode area surfaces, that is, source electrode 3 and drain electrode 4 to be gone
Ionized water, 10-8M、10-7M、10-6M、10-5M and 10-4M tobramycin solution, while electricity is recorded by semiconductor analysis instrument in real time
The situation of change of stream obtains real-time response curve and sees Fig. 2.It can be seen from the figure that in the antibiotic solution that each concentration is added dropwise
When, instantaneously increasing occur in source electrode 3 and 4 electric currents of drain electrode, and the response time less than 5 seconds, later in stabling current level, illustrates to pass
Sensor is fast to the detection speed of tobramycin, sensibility is high.
Embodiment 2
A kind of structure and manufacturing process such as embodiment 1 of the grapheme modified field effect transistor antibiotic sensor of aptamers
It is described.
A certain amount of 10 are prepared respectively-5The kanamycins of M concentration, streptomysin, Ciprofloxacin, tetracycline, tobramycin solution；
The sensor prepared is fixed on testboard, by the source electrode 3 of the syringe needle connection sensor of aptamers detection probe 7, drain electrode 4
On, semiconductor analysis instrument circuit is accessed, setting grid 1 is grounded, in the constant voltage of sensor both ends application+0.1V；Successively exist
(1 μ L) kanamycins, streptomysin, Ciprofloxacin, tetracycline, tobramycin solution is added dropwise in sensor sensing material load region,
The situation of change for passing through the real-time record current of semiconductor analysis instrument simultaneously, obtains real-time response curve and sees Fig. 3.It can from figure
Out, sensor includes kanamycins, streptomysin, ring to other antibiotic molecules with obvious responsing to tobramycin in water body
Third husky star and tetracycline do not respond significantly, illustrate that sensor is high to the selectivity of tobramycin, the detection to tobramycin
There are stronger anti-interference ability and resolving power.
Embodiment 3
A kind of structure and manufacturing process such as embodiment 1 of the grapheme modified field effect transistor antibiotic sensor of aptamers
It is described.
A certain amount of 10 are prepared respectively-8M、10-6M、10-4M tobramycin solution, with the pure buffer solution for being free of tobramycin
It is respectively placed in 4 syringes of multichannel micro-injection pump.The microfluidic sensing chip that will have been integrated with PDMS microchannel
It is fixed on 1 metal plate of grid, source electrode 3, drain electrode 4 and grid 1 respectively correspond in access semiconductor analysis instrument, and setting grid 1 connects
Ground, the constant voltage of application+0.1V between source electrode 3 and drain electrode 4；It by tobramycin concentrations is respectively 0M, 10 with syringe pump-8M、
10-6M、10-4The solution example to be measured of M is continuously injected into microfluidic sensing chip with the flow velocity of 50 μ L/min and difference is real-time
The situation of change of record, obtained real-time current curve are as shown in Figure 4.It can be seen from the figure that microfluidic sensing chip is to containing
Having the current water of various concentration tobramycin has the response signal obviously distinguished, signal value and static experimental result phase
Closely, illustrate that the microfluidic sensing chip can be using the measurement with tobramycin micro in current water.
The above description of the embodiments is intended to facilitate ordinary skill in the art to understand and use the invention.
Person skilled in the art obviously easily can make various modifications to these embodiments, and described herein general
Principle is applied in other embodiments without having to go through creative labor.Therefore, the present invention is not limited to the above embodiments, ability
Field technique personnel announcement according to the present invention, improvement and modification made without departing from the scope of the present invention all should be of the invention
Within protection scope.
Claims (9)
1. a kind of grapheme modified field effect transistor antibiotic sensor of aptamers, which is characterized in that including grid (1), source
Pole (3) and drain electrode (4), grid (1) surface is covered with single side oxidized silicon chip as electrode base sheet (2), the electrode base sheet (2)
Oxide layer area load have bridging source electrode (3) and the graphene layer (5) of (4) of draining, graphene layer (5) surface deposition are visited
Needle connector (6) is connected with aptamers detection probe (7) on the probe connector (6), by by source electrode (3), drain electrode (4)
Response signal is detected with grid (1) access semiconductor analysis instrument.
2. the grapheme modified field effect transistor antibiotic sensor of a kind of aptamers according to claim 1, feature
It is, the probe connector (6) is the nanogold particle being deposited on graphene layer (5).
3. the grapheme modified field effect transistor antibiotic sensor of a kind of aptamers according to claim 1, feature
It is, the aptamers detection probe (7) is aptamers RNA antibiotic probe.
4. the grapheme modified field effect transistor antibiotic sensor of a kind of aptamers according to claim 3, feature
It is, the aptamers RNA antibiotic probe chemical structure and nucleotides sequence are classified as HSC6-5 '-
GGCACGAGGUUUAGCUACACUCGUGCC-3’。
5. the grapheme modified field effect transistor antibiotic sensor of a kind of aptamers according to claim 1, feature
It is, the aptamers detection probe (7) is modified in such a way that solution hatches 12h on probe connector (6) surface.
6. the grapheme modified field effect transistor antibiotic sensor of a kind of aptamers according to claim 5, feature
It is, the solution is the PBS buffer solution containing three (2- carboxyethyl) phosphine disulfide bond activators, and adaptation bulk concentration is 20 μM.
7. the grapheme modified field effect transistor antibiotic sensor of a kind of aptamers according to claim 1, feature
It is, the oxidated layer thickness of the electrode base sheet (2) is 300nm.
8. the grapheme modified field effect transistor antibiotic sensor of a kind of aptamers according to claim 1, feature
It is, probe connector (6) exposed part is provided with 6- sulfydryl hex- 1- alcohol confining bed (9).
9. the grapheme modified field effect transistor antibiotic sensor of a kind of aptamers according to claim 1, feature
It is, the part of graphene layer (5) surface exposure is provided with 1- pyrene butanol confining bed (8).
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111999366A (en) * | 2020-08-14 | 2020-11-27 | 同济大学 | DNA modified molybdenum disulfide field effect transistor antibiotic sensor |
| CN112964766A (en) * | 2021-02-18 | 2021-06-15 | 同济大学 | Semiconductor resistance type nitrate detector |
| CN113219036A (en) * | 2021-04-08 | 2021-08-06 | 同济大学 | Silver ion sensor based on titanium carbide MXene field effect transistor and application thereof |
| CN113341128A (en) * | 2021-06-02 | 2021-09-03 | 江苏第二师范学院 | Biosensor for detecting tobramycin and detection method |
| CN113406172A (en) * | 2021-06-17 | 2021-09-17 | 哈尔滨工业大学 | Device for detecting myocardial infarction protein marker |
| CN115112628A (en) * | 2022-05-23 | 2022-09-27 | 德州学院 | SERS-FET dual-mode biosensor and application thereof in detection of microcystin-LR |
| CN115844386A (en) * | 2022-11-16 | 2023-03-28 | 哈尔滨工业大学（深圳） | Flexible sensor and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104345082A (en) * | 2013-08-06 | 2015-02-11 | 中国科学院苏州纳米技术与纳米仿生研究所 | Biological sensor, manufacturing method and detection method thereof |
| CN105353003A (en) * | 2015-10-19 | 2016-02-24 | 山东理工大学 | Microarray electrode-based detection method for antibiotic residues |
| CN106932376A (en) * | 2017-03-02 | 2017-07-07 | 江苏大学 | A kind of mycotoxin super sensitivity detection method of the gold silver core-shell nanometer rod based on DTNB marks |
| CN107389665A (en) * | 2017-06-23 | 2017-11-24 | 江南大学 | A kind of method based on aptamers modified nano gold colorimetric detection zearalenone |
| CN109870495A (en) * | 2019-01-30 | 2019-06-11 | 同济大学 | A kind of graphene-based field effect transistor nitrate sensor |
-
2019
- 2019-06-28 CN CN201910578201.2A patent/CN110346441A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104345082A (en) * | 2013-08-06 | 2015-02-11 | 中国科学院苏州纳米技术与纳米仿生研究所 | Biological sensor, manufacturing method and detection method thereof |
| CN105353003A (en) * | 2015-10-19 | 2016-02-24 | 山东理工大学 | Microarray electrode-based detection method for antibiotic residues |
| CN106932376A (en) * | 2017-03-02 | 2017-07-07 | 江苏大学 | A kind of mycotoxin super sensitivity detection method of the gold silver core-shell nanometer rod based on DTNB marks |
| CN107389665A (en) * | 2017-06-23 | 2017-11-24 | 江南大学 | A kind of method based on aptamers modified nano gold colorimetric detection zearalenone |
| CN109870495A (en) * | 2019-01-30 | 2019-06-11 | 同济大学 | A kind of graphene-based field effect transistor nitrate sensor |
Non-Patent Citations (1)
| Title |
|---|
| XIAOYAN CHEN等: "Ultratrace antibiotic sensing using aptamer/graphene-based field-effect transistors", 《BIOSENSORS AND BIOELECTRONICS》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111999366A (en) * | 2020-08-14 | 2020-11-27 | 同济大学 | DNA modified molybdenum disulfide field effect transistor antibiotic sensor |
| CN112964766A (en) * | 2021-02-18 | 2021-06-15 | 同济大学 | Semiconductor resistance type nitrate detector |
| CN113219036A (en) * | 2021-04-08 | 2021-08-06 | 同济大学 | Silver ion sensor based on titanium carbide MXene field effect transistor and application thereof |
| CN113341128A (en) * | 2021-06-02 | 2021-09-03 | 江苏第二师范学院 | Biosensor for detecting tobramycin and detection method |
| CN113341128B (en) * | 2021-06-02 | 2023-05-16 | 江苏第二师范学院 | Biosensor and detection method for detecting tobramycin |
| CN113406172A (en) * | 2021-06-17 | 2021-09-17 | 哈尔滨工业大学 | Device for detecting myocardial infarction protein marker |
| CN115112628A (en) * | 2022-05-23 | 2022-09-27 | 德州学院 | SERS-FET dual-mode biosensor and application thereof in detection of microcystin-LR |
| CN115844386A (en) * | 2022-11-16 | 2023-03-28 | 哈尔滨工业大学（深圳） | Flexible sensor and preparation method and application thereof |
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