CN110342640A - The production method for embedding device and fixed microorganism carrier - Google Patents
The production method for embedding device and fixed microorganism carrier Download PDFInfo
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- CN110342640A CN110342640A CN201910739425.7A CN201910739425A CN110342640A CN 110342640 A CN110342640 A CN 110342640A CN 201910739425 A CN201910739425 A CN 201910739425A CN 110342640 A CN110342640 A CN 110342640A
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- Prior art keywords
- pipe
- solution
- microorganism carrier
- strain
- linking agent
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- 244000005700 microbiome Species 0.000 title claims abstract description 59
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 22
- 241000894006 Bacteria Species 0.000 claims description 72
- 239000003431 cross linking reagent Substances 0.000 claims description 50
- 235000013379 molasses Nutrition 0.000 claims description 48
- 239000007788 liquid Substances 0.000 claims description 36
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 30
- 238000010438 heat treatment Methods 0.000 claims description 24
- 238000000034 method Methods 0.000 claims description 23
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 21
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 21
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical class OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 21
- 239000001110 calcium chloride Substances 0.000 claims description 21
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 21
- 239000001257 hydrogen Substances 0.000 claims description 21
- 229910052739 hydrogen Inorganic materials 0.000 claims description 21
- 230000001546 nitrifying effect Effects 0.000 claims description 21
- 238000002360 preparation method Methods 0.000 claims description 21
- 241000609240 Ambelania acida Species 0.000 claims description 18
- 239000010905 bagasse Substances 0.000 claims description 18
- DIZPMCHEQGEION-UHFFFAOYSA-H aluminium sulfate (anhydrous) Chemical compound [Al+3].[Al+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O DIZPMCHEQGEION-UHFFFAOYSA-H 0.000 claims description 15
- 239000000377 silicon dioxide Substances 0.000 claims description 15
- 235000012239 silicon dioxide Nutrition 0.000 claims description 15
- 239000012535 impurity Substances 0.000 claims description 14
- 238000001914 filtration Methods 0.000 claims description 12
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 12
- 230000001376 precipitating effect Effects 0.000 claims description 12
- 239000006228 supernatant Substances 0.000 claims description 11
- 238000001816 cooling Methods 0.000 claims description 10
- 239000004310 lactic acid Substances 0.000 claims description 6
- 235000014655 lactic acid Nutrition 0.000 claims description 6
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 229920002521 macromolecule Polymers 0.000 claims description 5
- 241000894007 species Species 0.000 claims description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims description 4
- 230000001590 oxidative effect Effects 0.000 claims description 4
- 241000235342 Saccharomycetes Species 0.000 claims description 2
- 230000003009 desulfurizing effect Effects 0.000 claims description 2
- 230000003247 decreasing effect Effects 0.000 claims 1
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 abstract description 7
- 239000004372 Polyvinyl alcohol Substances 0.000 abstract description 7
- 229920002451 polyvinyl alcohol Polymers 0.000 abstract description 7
- 235000010413 sodium alginate Nutrition 0.000 abstract description 7
- 239000000661 sodium alginate Substances 0.000 abstract description 7
- 229940005550 sodium alginate Drugs 0.000 abstract description 7
- 238000005273 aeration Methods 0.000 abstract description 3
- 238000004090 dissolution Methods 0.000 abstract description 3
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- 239000012153 distilled water Substances 0.000 description 16
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- 229920000642 polymer Polymers 0.000 description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 9
- 229910052799 carbon Inorganic materials 0.000 description 9
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 8
- 241000186660 Lactobacillus Species 0.000 description 8
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 8
- 229940039696 lactobacillus Drugs 0.000 description 8
- 239000001301 oxygen Substances 0.000 description 8
- 229910052760 oxygen Inorganic materials 0.000 description 8
- 230000006872 improvement Effects 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 240000000111 Saccharum officinarum Species 0.000 description 6
- 235000007201 Saccharum officinarum Nutrition 0.000 description 6
- 239000011575 calcium Substances 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 5
- 241000108664 Nitrobacteria Species 0.000 description 5
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 5
- 241001148470 aerobic bacillus Species 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 229910052791 calcium Inorganic materials 0.000 description 5
- 239000002131 composite material Substances 0.000 description 5
- 150000002500 ions Chemical class 0.000 description 5
- 230000000813 microbial effect Effects 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- 239000011591 potassium Substances 0.000 description 5
- 229910052700 potassium Inorganic materials 0.000 description 5
- 239000010865 sewage Substances 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- 229910021529 ammonia Inorganic materials 0.000 description 4
- 230000004071 biological effect Effects 0.000 description 4
- 229910001385 heavy metal Inorganic materials 0.000 description 4
- 239000002366 mineral element Substances 0.000 description 4
- 239000011259 mixed solution Substances 0.000 description 4
- 230000036284 oxygen consumption Effects 0.000 description 4
- 239000002504 physiological saline solution Substances 0.000 description 4
- 239000002861 polymer material Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000233866 Fungi Species 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000002035 prolonged effect Effects 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- 241000186361 Actinobacteria <class> Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 2
- 241001453382 Nitrosomonadales Species 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000000460 chlorine Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 238000007711 solidification Methods 0.000 description 2
- 230000008023 solidification Effects 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- 239000001124 (E)-prop-1-ene-1,2,3-tricarboxylic acid Substances 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241000588915 Klebsiella aerogenes Species 0.000 description 1
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- 235000018290 Musa x paradisiaca Nutrition 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 229940091181 aconitic acid Drugs 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 230000001651 autotrophic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- GTZCVFVGUGFEME-IWQZZHSRSA-N cis-aconitic acid Chemical compound OC(=O)C\C(C(O)=O)=C\C(O)=O GTZCVFVGUGFEME-IWQZZHSRSA-N 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000006477 desulfuration reaction Methods 0.000 description 1
- 230000023556 desulfurization Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229940092559 enterobacter aerogenes Drugs 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 1
- 235000013923 monosodium glutamate Nutrition 0.000 description 1
- 239000004223 monosodium glutamate Substances 0.000 description 1
- 238000006396 nitration reaction Methods 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
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- 239000006041 probiotic Substances 0.000 description 1
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- 230000035755 proliferation Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000001117 sulphuric acid Substances 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
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- 239000003440 toxic substance Substances 0.000 description 1
- GTZCVFVGUGFEME-UHFFFAOYSA-N trans-aconitic acid Natural products OC(=O)CC(C(O)=O)=CC(O)=O GTZCVFVGUGFEME-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biological Treatment Of Waste Water (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The present invention provides a kind of production method for embedding device and fixed microorganism carrier, wherein the embedding device includes: inner tube, the first intermediate tube, the second intermediate tube, outer tube.The present invention is prepared for novel fixed microorganism carrier by way of dual embedding.Enhance the resistance to aeration intensity of fixed microorganism carrier, high mechanical strength and resists influence of the environment processed to fixed microorganism carrier without the dissolution of polyvinyl alcohol and sodium alginate.Meanwhile the mechanical strength and bioactivity for slowing down the water-swellable of fixed microorganism carrier, improving carrier, extend the service life of carrier.To using the industrial-scale production of the achievable fixed microorganism carrier of the present invention, and then reduce the cost of wastewater treatment.
Description
Technical Field
The invention relates to sewage treatment, in particular to an embedding device with high solidification speed and high hardness after solidification and a manufacturing method of an immobilized microorganism carrier.
Background
The most fundamental technology of denitrification is not free from the degradation of microorganisms. The screened high-efficiency nitrifying bacteria and denitrifying bacteria are core strains in the biological denitrification technology of sewage. The biological denitrification technology firstly converts ammonia nitrogen into nitrate nitrogen through the nitrification of nitrifying bacteria, and then converts the nitrate nitrogen into nitrogen through the denitrification and discharges the nitrogen into the atmosphere, and finally realizes the circulation of nitrogen elements and the harmlessness of ammonia nitrogen.
The traditional biological denitrification technology has the following problems: firstly, because the concentration of dissolved oxygen is uneven due to different water depths, nitrifying bacteria (the nitrifying process is an oxygen consumption process) cannot be greatly enriched on the surface layer of the water, and thus the biological activity of the nitrifying bacteria is influenced; secondly, the effective microorganisms cannot survive in the water body or the survival time is too short due to the shortage of partial nutrient salts and carbon sources; a large amount of microorganisms precipitate into the sediment of the water body due to the gravity of the microorganisms, and lose the function of degrading dissolved nitrogen elements in the water body; fourthly, some macromolecule immobilized microorganism carriers can not be degraded by themselves, so that the water body generates secondary pollution.
The traditional biological denitrification process has the defects that autotrophic nitrifying bacteria cannot obtain growth advantages, higher biological concentration is difficult to maintain, investment and operation cost are higher, secondary pollution is easy to cause and the like. Therefore, it is necessary to provide a further solution to the above problems.
Disclosure of Invention
The invention aims to provide an embedding device and a manufacturing method of an immobilized microorganism carrier, so as to overcome the defects in the prior art.
To achieve the above object, the present invention provides an embedding apparatus comprising: the inner pipe, the first middle pipe, the second middle pipe and the outer pipe;
the inner pipe, the first middle pipe, the second middle pipe and the outer pipe are sequentially sleeved from inside to outside and communicated with each other, the lower end of the first middle pipe is fixed on the outer side wall of the inner pipe, the lower end of the second middle pipe is fixed on the outer side wall of the first middle pipe, the lower end of the outer pipe is fixed on the outer side wall of the second middle pipe, the inner pipe, the first middle pipe, the second middle pipe and the outer pipe are all connected with a liquid inlet pipe, an inlet is formed in the upper end of the inner pipe, and an outlet is formed in the lower end of the inner pipe.
As an improvement of the embedding device, the inner tube, the first middle tube, the second middle tube and the outer tube are all conical tubes with gradually reduced inner diameters from top to bottom.
As an improvement of the embedding device, the distance between the inner tube and the middle tube is 0.5-2 mm; the distance between the middle pipe and the outer pipe is 0.5-2 mm.
In order to achieve the above object, the present invention provides a method for producing an immobilized microorganism carrier, comprising producing the immobilized microorganism carrier by using the embedding apparatus as described above;
the manufacturing method comprises the following steps:
adding bagasse molasses solution from the inlet of the inner tube;
adding a first strain solution into the inner pipeline through a liquid inlet pipe;
adding a first cross-linking agent solution into a liquid inlet pipe of a first middle pipe;
adding a second strain solution into the liquid inlet pipe of the second middle pipe;
adding a second cross-linking agent solution into the liquid inlet pipe of the outer pipe;
adding a third cross-linking agent solution from the inlet of the inner tube;
the added solutions are polymerized to form the macromolecule spherical carrier embedded with the first strain solution and the second strain solution.
As an improvement of the preparation method of the immobilized microorganism carrier, the dosage of the bagasse molasses solution is 0.5-5 w%, and the preparation process comprises the following steps:
crushing bagasse, heating, juicing, cooling, and precipitating impurities;
filtering the supernatant, heating, squeezing, precipitating, and filtering;
repeating the steps for many times to obtain the blackish brown high-viscosity liquid molasses.
As an improvement of the method for producing the immobilized microorganism carrier of the present invention,
the first strain is denitrifying bacteria, and the second strain is nitrifying bacteria; or,
the first strain and the second strain are both hydrogen-producing bacteria; or,
the first and second strains are both lactic acid bacteria.
In the present invention, the first bacterial species and the second bacterial species are further selected from the group consisting of: bacillus, saccharomycete, strain of desulfurizing microbe, anaerobic ammonium oxidizing bacterium and aerobic ammonium oxidizing bacterium.
As an improvement of the method for producing the immobilized microorganism carrier of the present invention, the first crosslinking agent solution is: 0.3-5 wt% of calcium chloride and saturated boric acid, and 0.5-3 wt% of silicon dioxide solution.
As an improvement of the method for producing the immobilized microorganism carrier of the present invention, the second crosslinking agent solution is: 0.3-5 wt% of calcium chloride, saturated boric acid and 0.1-5 wt% of aluminum sulfate solution.
As an improvement of the method for producing the immobilized microorganism carrier of the present invention, the third crosslinking agent solution is: 0.3-5 wt% of calcium chloride and saturated boric acid, 0.5-3 wt% of silicon dioxide and 0.1-5 wt% of aluminum sulfate.
Compared with the prior art, the invention has the beneficial effects that: the invention prepares the novel immobilized microorganism carrier by a double embedding mode. The aeration resistance strength and the mechanical strength of the immobilized microorganism carrier are enhanced, the dissolution of polyvinyl alcohol and sodium alginate is avoided, and the influence of the treated environment on the immobilized microorganism carrier is resisted. Meanwhile, the water swelling capacity of the immobilized microorganism carrier is slowed down, the mechanical strength and the biological activity of the carrier are improved, and the service life of the carrier is prolonged. Therefore, the invention can realize the industrial scale production of the immobilized microorganism carrier, thereby reducing the cost of wastewater treatment.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments described in the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic plan view of an embedding apparatus of the present invention.
Detailed Description
The present invention is described in detail below with reference to various embodiments, but it should be understood that these embodiments are not intended to limit the present invention, and those skilled in the art should be able to make modifications and substitutions on the functions, methods, or structures of these embodiments without departing from the scope of the present invention.
The invention conception of the invention is as follows:
bagasse molasses is used as a carbon source and is supplied to the microorganisms through the slow release effect of the bagasse molasses. The anaerobic bacteria and the aerobic bacteria are immobilized on the same carrier, and the anaerobic bacteria are coated by the aerobic bacteria, so that a dissolved oxygen gradient is generated in the carrier. Aerobic bacteria are on the surface, and the dissolved oxygen is high; anaerobic bacteria are in the interior, and an anaerobic zone is formed due to the obstruction of oxygen transfer and the large oxygen consumption of external nitrifying bacteria. The cross-linking reaction is carried out for a plurality of times by adding the cross-linking agent containing silicon dioxide and aluminum sulfate, so that the water swelling property of the carrier is slowed down, the mechanical strength and the biological activity of the carrier are improved, and the service life of the carrier is prolonged.
Based on the above inventive concept, the present invention first provides an embedding device.
As shown in fig. 1, the embedding device of the present invention comprises: an inner tube 1, a first intermediate tube 2, a second intermediate tube 3, an outer tube 4.
The inner tube 1, the first middle tube 2, the second middle tube 3 and the outer tube 4 are sequentially sleeved from inside to outside and communicated with each other, the lower end of the first middle tube 2 is fixed on the outer side wall of the inner tube 1, the lower end of the second middle tube 3 is fixed on the outer side wall of the first middle tube 2, the lower end of the outer tube 4 is fixed on the outer side wall of the second middle tube 3, the inner tube 1, the first middle tube 2, the second middle tube 3 and the outer tube 4 are all connected with a liquid inlet tube, an inlet is formed in the upper end of the inner tube 1, and an outlet is formed in the lower end of the inner tube 1.
Preferably, the inner tube 1, the first middle tube 2, the second middle tube 3 and the outer tube 4 are all tapered tubes with gradually reduced inner diameters from top to bottom, and the distance between the inner tube 1 and the middle tubes is 0.5-2 mm; the distance between the middle pipe and the outer pipe 4 is 0.5-2 mm.
Through above-mentioned embedding device, multiple material is added in the import of accessible inner tube 1, the import of each feed liquor pipe, realizes immobilizing anaerobe and aerobic bacteria on same carrier, and the anaerobe is enveloped by aerobic bacteria for produce the dissolved oxygen gradient in the carrier.
The invention also provides a preparation method of the immobilized microorganism carrier, which adopts the embedding device to prepare the immobilized microorganism carrier.
The manufacturing method comprises the following steps:
adding bagasse molasses solution from the inlet of the inner tube;
adding a first strain solution into the inner pipeline through a liquid inlet pipe;
adding a first cross-linking agent solution into a liquid inlet pipe of a first middle pipe;
adding a second strain solution into the liquid inlet pipe of the second middle pipe;
adding a second cross-linking agent solution into the liquid inlet pipe of the outer pipe;
adding a third cross-linking agent solution from the inlet of the inner tube;
the added solutions are polymerized to form the macromolecule spherical carrier embedded with the first strain solution and the second strain solution. Wherein, each solution is dripped from the outlet of the inner tube into a magnetic stirring container by gravity to be polymerized into a spherical carrier with the diameter of 2-3mm, thereby ensuring the implementation of industrial scale production of the immobilized microorganism carrier.
The first strain is denitrifying bacteria, and the second strain is nitrifying bacteria; or both the first strain and the second strain are hydrogen-producing bacteria; alternatively, the first and second species are both lactic acid bacteria. In addition, the first and second bacterial species may be selected from the group consisting of: microorganisms used for sewage treatment, such as hydrogen-producing bacteria, lactic acid bacteria, bacillus, yeast, desulfurization microorganism strains, anaerobic ammonia oxidizing bacteria and aerobic ammonia oxidizing bacteria, functional microorganisms and probiotics used for aquaculture, and the like.
Molasses is a by-product of sugar industry using sugar cane as a raw material, and it is no longer economical to continuously extract sugar from molasses due to technical and cost reasons. The main component of molasses is sugar, and contains various nutritional components such as protein, natural minerals and vitamins. It is a dark brown, viscous liquid. The molasses is mainly used in feed industry, fermentation industry for producing alcohol, monosodium glutamate and the like, and adhesive for manufacturing industry and the like. Wherein the feed industry usage amount accounts for about 60% of world trade amount.
According to the current domestic banana molasses quality standard light industry standard (QB/T2684-. The cane molasses has different components due to different sugarcane varieties, maturity, planting climate, soil conditions, field management, sugar making process and the like, mainly contains moisture, sugar and non-sugar, wherein the sugar comprises cane sugar, reducing sugar and non-fermentable sugar, the non-sugar comprises organic matters, soluble gum and other carbohydrates, protein, amino acid, sodium, potassium, calcium, chlorine, phosphorus and other ions, and is rich and comprehensive in nutrition. The nutritional ingredients of the cane molasses are shown in table 1. Therefore, the bagasse molasses can be completely used as a carbon source and nutrient for sewage treatment.
TABLE 1 nutritional ingredient table of cane molasses
| Item | Content (%) |
| Moisture content | 25 |
| Dry fixation | 75 |
| Total sugar content | 48-56 |
| Sugar of cane | 30-40 |
| Reducing sugars | 15-20 |
| Non-fermentable sugar | 2-4 |
| Non-sugar organic matter | 9-12 |
| Aconitic acid | 3 |
| Protein | 8-10 |
| Ash of sulphuric acid | 10-15 |
| Na | 0.1-0.4 |
| K | 1.5-5.0 |
| Ca | 0.4-0.8 |
| Cl | 0.7-3.0 |
| P | 0.6-2.0 |
The first cross-linking agent solution is: 0.3-5 wt% of calcium chloride and saturated boric acid, and 0.5-3 wt% of silicon dioxide solution. The second cross-linking agent solution is: 0.3-5 wt% of calcium chloride, saturated boric acid and 0.1-5 wt% of aluminum sulfate solution. The third cross-linking agent solution is: 0.3-5 wt% of calcium chloride and saturated boric acid, 0.5-3 wt% of silicon dioxide and 0.1-5 wt% of aluminum sulfate.
The following will illustrate the technical scheme of the method for producing the immobilized microorganism carrier in connection with several examples.
Example 1
The process steps of this example are as follows:
1. treatment of bagasse molasses
Since cane molasses is complicated in composition and contains a large amount of non-sugar components and impurities (e.g., melanin, heavy metal ions, colloidal substances, etc.), the BOD and color of treated water are increased, and therefore, these impurities must be removed before the preparation of the immobilized microorganism carrier. Firstly, crushing bagasse, heating and squeezing, cooling, and precipitating impurities. Filtering the supernatant, heating and squeezing, precipitating, filtering, and repeating for multiple times to obtain blackish brown high-viscosity liquid molasses. The obtained molasses contains sugar, mineral elements such as calcium and potassium, and various amino acids, and is suitable for use as carbon source and nutrient for heterotrophic microorganisms such as denitrifying bacteria.
2. Preparation of concentrated denitrifying bacteria
Putting the denitrifying bacteria culture solution cultured for 48 h at constant temperature into a centrifuge tube, centrifuging at 3000 r/min for 10 min, discarding the supernatant, washing with sterile physiological saline, repeatedly centrifuging for 3 ~ 4 times to obtain concentrated denitrifying bacteria, and storing in a refrigerator at 4 deg.C for use.
3. Preparation of concentrated nitrifying bacteria
Putting the nitrobacteria culture solution cultured at constant temperature for 24 h into a centrifuge tube, centrifuging at 3000 r/min for 10 min, discarding the supernatant, washing with sterile physiological saline, repeatedly centrifuging for 3 ~ 4 times to obtain concentrated nitrobacteria, and storing in a refrigerator at 4 deg.C for use.
4. Preparation of polymer material for immobilization and cross-linking agent
(1) Preparing a polymer solution: accurately weighing 3g of PVA and 1.5g of sodium alginate, adding 100ml of distilled water, heating in a water bath at 40 ℃, stirring and pretreating for 2 hours; heating to 94 ℃, heating in a water bath until the solution is completely dissolved, and cooling to room temperature for later use;
(2) preparing a cross-linking agent 1, accurately weighing 20g of calcium chloride, saturated boric acid and 10g of silicon dioxide, adding into 1000ml of distilled water, and uniformly mixing for later use;
(3) preparing a cross-linking agent 2, accurately weighing 20g of calcium chloride, saturated boric acid and 10g of aluminum sulfate, adding into 1000ml of distilled water, and uniformly mixing for later use;
(4) preparing a cross-linking agent 3, accurately weighing 20g of calcium chloride, saturated boric acid, 10g of silicon dioxide and 10g of aluminum sulfate, adding into 1000ml of distilled water, and uniformly mixing for later use;
(5) preparing a sugar cane molasses solution: preparing a 3% processed cane molasses solution;
(6) preparing a denitrifying bacteria polymer mixed solution: weighing 3g of the concentrated denitrifying bacteria, adding the concentrated denitrifying bacteria into 100ml of the polymer solution, and uniformly mixing for later use;
(7) preparing a nitrifying bacteria high-molecular mixed solution: 3g of the concentrated nitrifying bacteria are weighed and added into 100ml of the polymer solution, and the mixture is uniformly mixed for later use.
5. Preparation of immobilized microorganism carrier
According to the method, cane molasses is added from an inlet 1 of a quadruple pipe, denitrifying bacteria high-molecular mixed liquid is added from an inlet 2, cross-linking agent 1 mixed liquid is added from an inlet 3, nitrifying bacteria high-molecular mixed liquid is added from an inlet 4, cross-linking agent 2 is added from an inlet 5, and finally, solution of the cross-linking agent 3 is dripped through an outlet 6 to polymerize into the high-molecular spherical carrier.
Example 2
In order to realize the microbial production of H2 as a new energy source for replacing coal and petroleum and the influence of waste water containing ammonia on hydrogen-producing bacteria, the waste water is treated and simultaneously new energy sources can be produced. By preparing the immobilized hydrogen-producing bacteria carrier, the tolerance of the hydrogen-producing bacteria to waste water containing ammonia is improved, and the hydrogen yield of hydrogen is improved, wherein the hydrogen yield of the culture by using the immobilized hydrogen-producing bacteria carrier is 3 times of the hydrogen yield of the culture without the immobilized hydrogen-producing bacteria carrier.
The process steps of this example are as follows:
1. treatment of bagasse molasses
Since cane molasses is complicated in composition and contains a large amount of non-sugar components and impurities (e.g., melanin, heavy metal ions, colloidal substances, etc.), the BOD and color of treated water are increased, and therefore, these impurities must be removed before the preparation of the immobilized microorganism carrier. Firstly, crushing bagasse, heating and squeezing, cooling, and precipitating impurities. Filtering the supernatant, heating and squeezing, precipitating, filtering, and repeating for multiple times to obtain blackish brown high-viscosity liquid molasses. The obtained molasses contains sugar, mineral elements such as calcium and potassium, and various amino acids, and is suitable for use as carbon source and nutrient for heterotrophic microorganisms such as denitrifying bacteria.
2. Preparation of concentrated hydrogen-producing bacteria (Enterobacter aerogenes)
Putting the hydrogen-producing bacteria culture solution cultured at constant temperature for 48 h into a centrifuge tube, centrifuging at 3000 r/min for 10 min, discarding the supernatant, washing with sterile physiological saline, repeatedly centrifuging for 3 ~ 4 times to obtain concentrated hydrogen-producing bacteria, and storing in a refrigerator at 4 deg.C for use.
3. Preparation of polymer material for immobilization and cross-linking agent
(1) Preparing a polymer solution: accurately weighing 3g of PVA and 1.5g of sodium alginate, adding 100ml of distilled water, heating in a water bath at 40 ℃, stirring and pretreating for 2 hours; heating to 94 ℃, heating in a water bath until the solution is completely dissolved, and cooling to room temperature for later use;
(2) preparing a cross-linking agent 1, accurately weighing 20g of calcium chloride, saturated boric acid and 10g of silicon dioxide, adding into 1000ml of distilled water, and uniformly mixing for later use;
(3) preparing a cross-linking agent 2, accurately weighing 20g of calcium chloride, saturated boric acid and 10g of aluminum sulfate, adding into 1000ml of distilled water, and uniformly mixing for later use;
(4) preparing a cross-linking agent 3, accurately weighing 20g of calcium chloride, saturated boric acid, 10g of silicon dioxide and 10g of aluminum sulfate, adding into 1000ml of distilled water, and uniformly mixing for later use;
(5) preparing a sugar cane molasses solution: preparing a 3% processed cane molasses solution;
(6) preparing hydrogen-producing bacteria macromolecule mixed liquid: weighing 3g of concentrated hydrogen-producing bacteria, adding the hydrogen-producing bacteria into 100ml of polymer solution, and uniformly mixing for later use;
4. preparation of immobilized microorganism carrier
According to the method, cane molasses is added from an inlet 1 of a quadruple pipe and hydrogen-producing bacteria high-molecular mixed liquid is added from an inlet 2 through a micro-infusion pump, cross-linking agent 1 mixed liquid is added from an inlet 3, hydrogen-producing bacteria high-molecular mixed liquid is added from an inlet 4, cross-linking agent 2 and cross-linking agent 2 are added from an inlet 5, and finally, solution of the cross-linking agent 3 is dripped through an outlet 6 to polymerize into the high-molecular spherical carrier.
Example 3
The immobilized lactobacillus carrier is prepared, is used as a feed microbial additive to be applied to the cultured animals in an oral mode to improve the immunity of aquatic animals, and is also used as a water body microecological regulator to improve the water quality.
The process steps of this example are as follows:
1. treatment of bagasse molasses
Since cane molasses is complicated in composition and contains a large amount of non-sugar components and impurities (e.g., melanin, heavy metal ions, colloidal substances, etc.), the BOD and color of treated water are increased, and therefore, these impurities must be removed before the preparation of the immobilized microorganism carrier. Firstly, crushing bagasse, heating and squeezing, cooling, and precipitating impurities. Filtering the supernatant, heating and squeezing, precipitating, filtering, and repeating for multiple times to obtain blackish brown high-viscosity liquid molasses. The obtained molasses contains sugar, mineral elements such as calcium and potassium, and various amino acids, and is suitable for use as carbon source and nutrient for heterotrophic microorganisms such as denitrifying bacteria.
2. Preparation of concentrated lactic acid bacteria
Putting the lactobacillus culture solution cultured at constant temperature for 48 h into a centrifuge tube, centrifuging at 3000 r/min for 10 min, discarding the supernatant, washing with sterile physiological saline, repeatedly centrifuging for 3 ~ 4 times to obtain concentrated lactobacillus, and storing in a refrigerator at 4 deg.C.
3. Preparation of polymer material for immobilization and cross-linking agent
(1) Preparing a polymer solution: accurately weighing 3g of PVA and 1.5g of sodium alginate, adding 100ml of distilled water, heating in a water bath at 40 ℃, stirring and pretreating for 2 hours; heating to 94 ℃, heating in a water bath until the solution is completely dissolved, and cooling to room temperature for later use;
(2) preparing a cross-linking agent 1, accurately weighing 20g of calcium chloride, saturated boric acid and 10g of silicon dioxide, adding into 1000ml of distilled water, and uniformly mixing for later use;
(3) preparing a cross-linking agent 2, accurately weighing 20g of calcium chloride, saturated boric acid and 10g of aluminum sulfate, adding into 1000ml of distilled water, and uniformly mixing for later use;
(4) preparing a cross-linking agent 3, accurately weighing 20g of calcium chloride, saturated boric acid, 10g of silicon dioxide and 10g of aluminum sulfate, adding into 1000ml of distilled water, and uniformly mixing for later use;
(5) preparing a sugar cane molasses solution: preparing a 3% processed cane molasses solution;
(6) preparing a lactic acid bacteria polymer mixed solution: weighing 3g of concentrated lactobacillus, adding into 100ml of polymer solution, and mixing uniformly for later use;
4. preparation of immobilized microorganism carrier
According to the method, cane molasses is added from an inlet 1 of a quadruple pipe, lactobacillus high-molecular mixed liquid is added from an inlet 2, cross-linking agent 1 mixed liquid is added from an inlet 3, lactobacillus high-molecular mixed liquid is added from an inlet 4, cross-linking agent 2 and cross-linking agent 2 are added from an inlet 5, and finally, solution of the cross-linking agent 3 is dripped through an outlet 6 to polymerize into the high-molecular spherical carrier.
Example 4
Preparing an immobilized composite microbial carrier as an aquatic ecological environment regulating agent, and using the immobilized composite microbial carrier as a sewage treatment plant for upgrading, improving the efficiency of a biochemical system, performing advanced treatment, removing nitrogen and phosphorus, and reducing sludge in the process; meanwhile, the method can also be used for treating black and odorous water and restoring micro-ecology.
The process steps of this example are as follows:
1. treatment of bagasse molasses
Since cane molasses is complicated in composition and contains a large amount of non-sugar components and impurities (e.g., melanin, heavy metal ions, colloidal substances, etc.), the BOD and color of treated water are increased, and therefore, these impurities must be removed before the preparation of the immobilized microorganism carrier. Firstly, crushing bagasse, heating and squeezing, cooling, and precipitating impurities. Filtering the supernatant, heating and squeezing, precipitating, filtering, and repeating for multiple times to obtain blackish brown high-viscosity liquid molasses. The obtained molasses contains sugar, mineral elements such as calcium and potassium, and various amino acids, and is suitable for use as carbon source and nutrient for heterotrophic microorganisms such as denitrifying bacteria.
2. Preparation of composite microbial liquid
Putting culture solution of composite microorganism (mainly including nitrobacteria, bacillus, mould, actinomycetes, fungi and filamentous fungi) cultured at constant temperature for 48 h into a centrifuge tube, centrifuging at 3000 r/min for 10 min, discarding supernatant, washing with sterile normal saline, repeatedly centrifuging for 3 ~ 4 times to obtain bacterial solution, and storing in a refrigerator at 4 deg.C for use.
3. Preparation of polymer material for immobilization and cross-linking agent
(1) Preparing a polymer solution: accurately weighing 3g of PVA and 1.5g of sodium alginate, adding 100ml of distilled water, heating in a water bath at 40 ℃, stirring and pretreating for 2 hours; heating to 94 ℃, heating in a water bath until the solution is completely dissolved, and cooling to room temperature for later use;
(2) preparing a cross-linking agent 1, accurately weighing 20g of calcium chloride, saturated boric acid and 10g of silicon dioxide, adding into 1000ml of distilled water, and uniformly mixing for later use;
(3) preparing a cross-linking agent 2, accurately weighing 20g of calcium chloride, saturated boric acid and 10g of aluminum sulfate, adding into 1000ml of distilled water, and uniformly mixing for later use;
(4) preparing a cross-linking agent 3, accurately weighing 20g of calcium chloride, saturated boric acid, 10g of silicon dioxide and 10g of aluminum sulfate, adding into 1000ml of distilled water, and uniformly mixing for later use;
(5) preparing a sugar cane molasses solution: preparing a 3% processed cane molasses solution;
(6) preparing a composite bacteria polymer mixed solution: weighing 3g of concentrated compound bacteria (mainly including nitrobacteria, bacillus, mould, actinomycetes, fungi and filamentous bacteria) and adding into 100ml of polymer solution, and mixing uniformly for later use;
4. preparation of immobilized microorganism carrier
According to the method, cane molasses is added from an inlet 1 of a quadruple pipe, lactobacillus high-molecular mixed liquid is added from an inlet 2, cross-linking agent 1 mixed liquid is added from an inlet 3, lactobacillus high-molecular mixed liquid is added from an inlet 4, cross-linking agent 2 and cross-linking agent 2 are added from an inlet 5, and finally, solution of the cross-linking agent 3 is dripped through an outlet 6 to polymerize into the high-molecular spherical carrier.
In conclusion, the invention prepares the novel immobilized microorganism carrier in a double embedding mode. The aeration resistance strength and the mechanical strength of the immobilized microorganism carrier are enhanced, the dissolution of polyvinyl alcohol and sodium alginate is avoided, and the influence of the treated environment on the immobilized microorganism carrier is resisted. Meanwhile, the water swelling capacity of the immobilized microorganism carrier is slowed down, the mechanical strength and the biological activity of the carrier are improved, and the service life of the carrier is prolonged. Therefore, the invention can realize the industrial scale production of the immobilized microorganism carrier, thereby reducing the cost of wastewater treatment.
Furthermore, the invention utilizes the characteristic that the denitrifying bacteria and the nitrifying bacteria are immobilized on the same carrier, and the denitrifying bacteria are coated by the nitrifying bacteria, so that the immobilized microorganism carrier generates a dissolved oxygen gradient: the nitrifying bacteria are on the surface, the dissolved oxygen is high, and the oxygen consumption is high to realize the nitration reaction; inside the denitrifying bacteria, an anaerobic zone is formed due to the obstructed oxygen transfer and the large oxygen consumption of external nitrifying bacteria, so that anaerobic denitrification reaction is realized. Because the denitrification rate is relatively low when methanol is used as a carbon source, the traditional addition method is not only complicated, but also easy to form secondary pollution. The cane molasses is used for replacing methanol, and a carbon source is continuously provided for denitrifying bacteria through the slow-release dissolving process of the cane molasses, so that the high-density proliferation and the high-efficiency denitrification treatment of the denitrifying bacteria are guaranteed. Nitrifying bacteria have strong removal capacity to ammonia and nitrite in water, but because the nitrifying bacteria belong to chemoautotrophic bacteria, the growth period is long, the nitrifying bacteria are sensitive to environment-factor change, and are in disadvantage in competition with heterotrophic bacteria. The application of the immobilized nitrobacteria technology can improve the impact resistance of the system to toxic substances, and the efficient removal of ammonia and nitrite in the water body has great significance for water ecological health and environmental protection.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (10)
1. An embedding apparatus, characterized in that the embedding apparatus comprises: the inner pipe, the first middle pipe, the second middle pipe and the outer pipe;
the inner pipe, the first middle pipe, the second middle pipe and the outer pipe are sequentially sleeved from inside to outside and communicated with each other, the lower end of the first middle pipe is fixed on the outer side wall of the inner pipe, the lower end of the second middle pipe is fixed on the outer side wall of the first middle pipe, the lower end of the outer pipe is fixed on the outer side wall of the second middle pipe, the inner pipe, the first middle pipe, the second middle pipe and the outer pipe are all connected with a liquid inlet pipe, an inlet is formed in the upper end of the inner pipe, and an outlet is formed in the lower end of the inner pipe.
2. The embedding device of claim 1, wherein the inner tube, the first intermediate tube, the second intermediate tube, and the outer tube are tapered tubes with gradually decreasing inner diameters from top to bottom.
3. Embedding device according to claim 1, characterized in that the distance between the inner tube and the intermediate tube is 0.5-2 mm; the distance between the middle pipe and the outer pipe is 0.5-2 mm.
4. A method for producing an immobilized microorganism carrier, which comprises producing the immobilized microorganism carrier by using the entrapping device according to any one of claims 1 to 3;
the manufacturing method comprises the following steps:
adding bagasse molasses solution from the inlet of the inner tube;
adding a first strain solution into the inner pipeline through a liquid inlet pipe;
adding a first cross-linking agent solution into a liquid inlet pipe of a first middle pipe;
adding a second strain solution into the liquid inlet pipe of the second middle pipe;
adding a second cross-linking agent solution into the liquid inlet pipe of the outer pipe;
adding a third cross-linking agent solution from the inlet of the inner tube;
the added solutions are polymerized to form the macromolecule spherical carrier embedded with the first strain solution and the second strain solution.
5. The method for preparing the immobilized microorganism carrier according to claim 1, wherein the bagasse molasses solution is used in an amount of 0.5-5 w%, and the preparation process comprises:
crushing bagasse, heating, juicing, cooling, and precipitating impurities;
filtering the supernatant, heating, squeezing, precipitating, and filtering;
repeating the steps for many times to obtain the blackish brown high-viscosity liquid molasses.
6. The method of producing an immobilized microorganism carrier according to claim 1,
the first strain is denitrifying bacteria, and the second strain is nitrifying bacteria; or,
the first strain and the second strain are both hydrogen-producing bacteria; or,
the first and second strains are both lactic acid bacteria.
7. The method for producing an immobilized microorganism carrier according to claim 1, wherein the first and second bacterial species are further selected from the group consisting of: bacillus, saccharomycete, strain of desulfurizing microbe, anaerobic ammonium oxidizing bacterium and aerobic ammonium oxidizing bacterium.
8. The method of claim 1, wherein the first cross-linking agent solution is: 0.3-5 wt% of calcium chloride and saturated boric acid, and 0.5-3 wt% of silicon dioxide solution.
9. The method of claim 1, wherein the second cross-linking agent solution is: 0.3-5 wt% of calcium chloride, saturated boric acid and 0.1-5 wt% of aluminum sulfate solution.
10. The method of claim 1, wherein the third cross-linking agent solution is: 0.3-5 wt% of calcium chloride and saturated boric acid, 0.5-3 wt% of silicon dioxide and 0.1-5 wt% of aluminum sulfate.
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