CN110339400A - The anticalcium processing method of biomaterial - Google Patents

The anticalcium processing method of biomaterial Download PDF

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Publication number
CN110339400A
CN110339400A CN201910723767.XA CN201910723767A CN110339400A CN 110339400 A CN110339400 A CN 110339400A CN 201910723767 A CN201910723767 A CN 201910723767A CN 110339400 A CN110339400 A CN 110339400A
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CN
China
Prior art keywords
biomaterial
anticalcium
processing method
immersed
soaking
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Application number
CN201910723767.XA
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Chinese (zh)
Inventor
陈华成
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TAICANG XINYAXUN BIOTECHNOLOGY Co Ltd
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TAICANG XINYAXUN BIOTECHNOLOGY Co Ltd
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Priority to CN201910723767.XA priority Critical patent/CN110339400A/en
Publication of CN110339400A publication Critical patent/CN110339400A/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/20—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/222—Gelatin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/24—Collagen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/362—Skin, e.g. dermal papillae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/3625—Vascular tissue, e.g. heart valves
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2400/00—Materials characterised by their function or physical properties
    • A61L2400/02—Treatment of implants to prevent calcification or mineralisation in vivo

Abstract

The invention discloses a kind of anticalcium processing methods of biomaterial, comprising the following steps: S1, immerses the biomaterial in cross-linking agent solution, soaking temperature is 20-30 DEG C, soaking time 4-6d；S2, the biomaterial is immersed in glutaraldehyde solution, soaking temperature is 20-25 DEG C, soaking time 2-3d；S3, the biomaterial is immersed in microemulsion, soaking temperature is 20-25 DEG C, soaking time 72h.By the above-mentioned means, the present invention enhances the anticalcium effect of biomaterial under the premise of guaranteeing biomaterial structurally and mechanically mechanical property, and then improve the service life of biomaterial.

Description

The anticalcium processing method of biomaterial
Technical field
The invention belongs to bio-medical technology fields, more particularly to a kind of anticalcium processing method of biomaterial.
Background technique
Biomaterial refer to it is a kind of contact with each other with biosystem after can tissue, organ or function to organism examine Disconnected, treatment can enhance or alternative material.Biomaterial nowadays has been widely used for every field, but due to existing The problem of biocompatibility, biomaterial are implanted into human body a series of problems, such as there is infection, tumour and pathologic calcifications.
Calcification, which refers to, there is undesirable calcium phosphate deposition on the surface of implantation material and inside, these lesions may shadow Ring heart valve, pacemaker, urethra reparation soft contact lens property.In the formation of marrow, enamel and dentine, Calcification is normally, to belong to physiological, but the doped calcium in soft tissue is improper useless.
Summary of the invention
The invention mainly solves the technical problem of providing a kind of anticalcium processing methods of biomaterial, are guaranteeing biology Enhance the anticalcium effect of biomaterial under the premise of material structure and mechanical mechanics property, and then improves the use of biomaterial Service life.
In order to solve the above technical problems, one technical scheme adopted by the invention is that: a kind of anticalcium of biomaterial is provided Change processing method, comprising the following steps:
S1, the biomaterial is immersed in cross-linking agent solution, soaking temperature is 20-30 DEG C, soaking time 4-6d；
S2, the biomaterial is immersed in glutaraldehyde solution, soaking temperature is 20-25 DEG C, soaking time 2-3d；
S3, the biomaterial is immersed in microemulsion, soaking temperature is 20-25 DEG C, soaking time 72h.
It further says, the cross-linking agent solution is 1- ethyl -3- (3- dimethylamino-propyl) carbodiimides and N- hydroxyl The mixed liquor of base succinimide, 1- ethyl -3- (3- dimethylamino-propyl) carbodiimides and the N- hydroxysuccinimidyl Imido mixing mass ratio is 3:1.
It further says, the concentration of the glutaraldehyde solution is 0.3-0.6%, pH value 6.0-7.0.
It further says, the microemulsion includes lauryl sodium sulfate, potassium oleate, enuatrol, potassium stearate, poly- second two At least two in alcohol octyl phenyl ether, polyoxyethylene groups sorbitan ester and sorbitan ester.
It further says, the biomaterial is animal tissue or artificial bio-membrane's material made of natural macromolecular material Material.
It further says, the animal tissue is pericardium, valve, goldbeater's skin, meninx, lung film, blood vessel, skin or ligament.
It further says, the natural macromolecular material is collagen, elastin laminin, hyaluronic acid and gelatin.
The beneficial effects of the present invention are: the present invention enhances under the premise of guaranteeing biomaterial structurally and mechanically mechanical property The anticalcium effect of biomaterial, and then improve the service life of biomaterial.
Specific embodiment
The preferred embodiments of the present invention will be described in detail below so that advantages and features of the invention can be easier to by It will be appreciated by those skilled in the art that so as to make a clearer definition of the protection scope of the present invention.
A kind of embodiment: anticalcium processing method of biomaterial, comprising the following steps:
S1, the biomaterial is immersed in cross-linking agent solution, soaking temperature is 20-30 DEG C, soaking time 4-6d；
S2, the biomaterial is immersed in glutaraldehyde solution, soaking temperature is 20-25 DEG C, soaking time 2-3d；
S3, the biomaterial is immersed in microemulsion, soaking temperature is 20-25 DEG C, soaking time 72h.
The cross-linking agent solution is 1- ethyl -3- (3- dimethylamino-propyl) carbodiimides and n-hydroxysuccinimide Mixed liquor, the mixing of 1- ethyl -3- (3- dimethylamino-propyl) carbodiimides and the n-hydroxysuccinimide Mass ratio is 3:1.
The concentration of the glutaraldehyde solution is 0.3-0.6%, pH value 6.0-7.0.
The microemulsion includes lauryl sodium sulfate, potassium oleate, enuatrol, potassium stearate, polyethylene glycol octyl phenyl At least two in ether, polyoxyethylene groups sorbitan ester and sorbitan ester.
The biomaterial is animal tissue or the bioartificial materials made of natural macromolecular material.
The animal tissue is pericardium, valve, goldbeater's skin, meninx, lung film, blood vessel, skin or ligament.
The natural macromolecular material is collagen, elastin laminin, hyaluronic acid and gelatin.
The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair Equivalent structure transformation made by bright specification is applied directly or indirectly in other relevant technical fields, and is similarly included in In scope of patent protection of the invention.

Claims (7)

1. a kind of anticalcium processing method of biomaterial, it is characterised in that: the following steps are included:
S1, the biomaterial is immersed in cross-linking agent solution, soaking temperature is 20-30 DEG C, soaking time 4-6d；
S2, the biomaterial is immersed in glutaraldehyde solution, soaking temperature is 20-25 DEG C, soaking time 2-3d；
S3, the biomaterial is immersed in microemulsion, soaking temperature is 20-25 DEG C, soaking time 72h.
2. a kind of anticalcium processing method of biomaterial according to claim 1, it is characterised in that: the crosslinking agent is molten Liquid is the mixed liquor of 1- ethyl -3- (3- dimethylamino-propyl) carbodiimides and n-hydroxysuccinimide, the 1- ethyl - The mixing mass ratio of 3- (3- dimethylamino-propyl) carbodiimides and the n-hydroxysuccinimide is 3:1.
3. a kind of anticalcium processing method of biomaterial according to claim 1, it is characterised in that: the glutaraldehyde is molten The concentration of liquid is 0.3-0.6%, pH value 6.0-7.0.
4. a kind of anticalcium processing method of biomaterial according to claim 1, it is characterised in that: the microemulsion packet Include lauryl sodium sulfate, potassium oleate, enuatrol, potassium stearate, Triton X-100, polyoxyethylene groups Sorbitan At least two in alcohol ester and sorbitan ester.
5. a kind of anticalcium processing method of biomaterial according to claim 1, it is characterised in that: the biomaterial For animal tissue or the bioartificial materials made of natural macromolecular material.
6. a kind of anticalcium processing method of biomaterial according to claim 1, it is characterised in that: the animal tissue For pericardium, valve, goldbeater's skin, meninx, lung film, blood vessel, skin or ligament.
7. a kind of anticalcium processing method of biomaterial according to claim 1, it is characterised in that: the natural polymer Sub- material is collagen, elastin laminin, hyaluronic acid and gelatin.
CN201910723767.XA 2019-08-07 2019-08-07 The anticalcium processing method of biomaterial Withdrawn CN110339400A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004006974A1 (en) * 2002-07-16 2004-01-22 Edwards Lifesciences Corporation Enhanced phospholipid reduction and calcification mitigation of biological materials
CN105363071A (en) * 2015-11-23 2016-03-02 金仕生物科技（常熟）有限公司 Anti-calcification treatment method for biological materials
CN108785747A (en) * 2018-07-07 2018-11-13 四川大学 A kind of processing method and its biomaterial improving biomaterial anticalcium performance
CN109125810A (en) * 2017-06-19 2019-01-04 上海微创心通医疗科技有限公司 A kind of preparation method of biovalve
CN109260517A (en) * 2018-09-19 2019-01-25 杭州启明医疗器械有限公司 One kind can pre-install dry biological cardiac valves and preparation method thereof
CN109513048A (en) * 2018-10-09 2019-03-26 沛嘉医疗科技(苏州)有限公司 A kind of cross-linking method of bioartificial tissue

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004006974A1 (en) * 2002-07-16 2004-01-22 Edwards Lifesciences Corporation Enhanced phospholipid reduction and calcification mitigation of biological materials
CN105363071A (en) * 2015-11-23 2016-03-02 金仕生物科技（常熟）有限公司 Anti-calcification treatment method for biological materials
CN109125810A (en) * 2017-06-19 2019-01-04 上海微创心通医疗科技有限公司 A kind of preparation method of biovalve
CN108785747A (en) * 2018-07-07 2018-11-13 四川大学 A kind of processing method and its biomaterial improving biomaterial anticalcium performance
CN109260517A (en) * 2018-09-19 2019-01-25 杭州启明医疗器械有限公司 One kind can pre-install dry biological cardiac valves and preparation method thereof
CN109513048A (en) * 2018-10-09 2019-03-26 沛嘉医疗科技(苏州)有限公司 A kind of cross-linking method of bioartificial tissue

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