CN110338222A - A kind of high-purity conjugated linoleic acid yak yoghourt and preparation method thereof - Google Patents
A kind of high-purity conjugated linoleic acid yak yoghourt and preparation method thereof Download PDFInfo
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- CN110338222A CN110338222A CN201910784940.7A CN201910784940A CN110338222A CN 110338222 A CN110338222 A CN 110338222A CN 201910784940 A CN201910784940 A CN 201910784940A CN 110338222 A CN110338222 A CN 110338222A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/1203—Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
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Abstract
A kind of high-purity conjugated linoleic acid yak yoghourt and preparation method thereof, is related to dairy products field, the preparation method first in yak milk fat and protein digest, be translated into the small molecule compound more conducively absorbed, then fermentation operation is carried out to enzymolysis liquid.Such enzymatic hydrolysis includes two effects.First, the more conventional milk of Oil content and Protein content in yak milk is higher, in order to guarantee its mouthfeel, need to be reduced to and the comparable level of conventional milk；Secondly, the present invention selects porcine pancreatic lipase to carry out when digesting to lipase, and porcine pancreatic lipase has specificity for the fat containing unsaturated linoleic acid structure, to release conjugated linoleic acid from lipid structure, increase the cla levels of yak milk entirety.Its is easy to operate, and the yak yoghourt being prepared, milk is strong, taste good, and cla levels are high.With more significant anticancer, anti-oxidant, reduction blood lipid, nutritive value is abundant, is suitble to people's long-term drinking.
Description
Technical field
The present invention relates to dairy products fields, in particular to a kind of high-purity conjugated linoleic acid yak yoghourt and its preparation
Method.
Background technique
Yak is the main poultry kind of Qinghai-xizang Plateau Region, and the yak milk of production accounts for Qinghai-xizang Plateau Region milk consumption figure
90%.Compared to other milk, fat, protein, lactose, vitamin and essential amino acids content are higher in yak milk, fatty acid
Type is more, and cholesterol level is lower, is known as " superfine product in milk ".Especially in recent years, about conjugated linoleic acid the effect of, is gradually
It is recognized by people, as one of highest milk kind of cla levels, yak milk being paid attention to by people more.
Conjugated linoleic acid is a series of general name of octadecadienoic acids containing conjugated double bond.Studies have shown that the sub- oil of conjugation
Acid has the multiple efficacies such as anticancer, anti-oxidant, reduction cholesterol, strengthen immunity.However, conjugated linoleic acid contains in nature
It measures less, is primarily present in fat and its dairy products with ruminant ox and sheep.Wherein, in common milk cow's milk, it is conjugated sub- oil
The content of acid only has 0.05 ~ 0.1mg/ml, in contrast, 0.5 ~ 0.6 mg/ml is then up in yak milk.Make conjugated linoleic acid
(CLA) its latent effect being played to human health, the mankind daily intake the amount of conjugated linoleic acid (CLA), at least need 3g daily,
In west using dairy products and animal product as the country of staple food, practical intake is also less than the one third of recommended amounts per capita, always
For body, or far from meeting the needs of people, develops high-purity conjugated linoleic acid (CLA) yak yoghourt and have a vast market
Prospect.
In the prior art, it is desirable to the content for increasing conjugated linoleic acid in dairy products generallys use the mode of external addition, but
It is the cost that this method will increase extra purchase conjugated linoleic acid.
Summary of the invention
The first object of the present invention is to provide a kind of preparation method of high-purity conjugated linoleic acid yak yoghourt, can be
Guarantee yoghourt-flavored, and not additionally add conjugated linoleic acid in the case where, improve Yoghourt in cla levels, reduce production
Cost.Its is easy to operate, not high to equipment requirement, is appropriate for industrialization promotion.
The second object of the present invention is to provide a kind of high-purity conjugated linoleic acid yak yoghourt, by above-mentioned preparation method system
It is standby to obtain.The milk of high-purity conjugated linoleic acid yak yoghourt is strong, taste good.Meanwhile cla levels are high, tool
There are more significant anticancer, anti-oxidant, reduction blood lipid, balancing blood pressure, weight-reducing to prevent the function such as diabetes generation, strengthen immunity
Effect, nutritive value is abundant, is suitble to people's long-term drinking.
The embodiment of the present invention is achieved in that
A kind of preparation method of high-purity conjugated linoleic acid yak yoghourt comprising:
By yak milk and porcine pancreatic lipase 3 ~ 8 h of mixed enzymolysis at 35 ~ 40 DEG C by filtration sterilization processing, the first enzyme is obtained
Solve liquid；
First enzymolysis liquid is heated to 90 ~ 100 DEG C, 10 ~ 15 min is maintained to be inactivated；
By the first enzymolysis liquid and protease 2 ~ 5 h of mixed enzymolysis at 38 ~ 43 DEG C after inactivation, the second enzymolysis liquid is obtained；
Second enzymolysis liquid is heated to 90 ~ 100 DEG C, 10 ~ 15 min is maintained to be inactivated；
The second enzymolysis liquid after inactivation is mixed with xylitol, emulsifier, by homogeneous, sterilization, obtains fermenation raw liquid；
Zymophyte is accessed in fermenation raw liquid to ferment.
A kind of high-purity conjugated linoleic acid yak yoghourt, by the preparation method system of above-mentioned high-purity conjugated linoleic acid yak yoghourt
It is standby to obtain.
The beneficial effect of the embodiment of the present invention is:
The embodiment of the invention provides a kind of preparation methods of high-purity conjugated linoleic acid yak yoghourt, first to the rouge in yak milk
Fat and protein are digested, and are translated into the small molecule compound more conducively absorbed, then carry out fermentation operation to enzymolysis liquid.
Such enzymatic hydrolysis includes two effects.First, the more conventional milk of Oil content and Protein content in yak milk is higher, in order to guarantee
Its mouthfeel needs to be reduced to and the comparable level of conventional milk；Second, the present invention when digesting to lipase, selects
Porcine pancreatic lipase progress is selected, porcine pancreatic lipase has specificity for the fat containing unsaturated linoleic acid structure, thus from rouge
Conjugated linoleic acid is released in fat structure, increases whole cla levels.The preparation method it is easy to operate, to equipment
It is of less demanding, it is appropriate for industrialization promotion.
The embodiment of the invention also provides a kind of high-purity conjugated linoleic acid yak yoghourts, are prepared by above-mentioned preparation method
It arrives.The milk of high-purity conjugated linoleic acid yak yoghourt is strong, taste good.Meanwhile there is more significant anticancer, antioxygen
Change, reduction blood lipid, balancing blood pressure, weight-reducing prevent diabetes generation, strengthen immunity and other effects, and nutritive value is abundant, is suitble to people
Long-term drinking.
Specific embodiment
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, according to normal conditions or manufacturer builds
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
A kind of preparation method of high-purity conjugated linoleic acid yak yoghourt of the embodiment of the present invention is specifically described below.
The embodiment of the invention provides a kind of preparation methods of high-purity conjugated linoleic acid yak yoghourt, mainly there is enzymatic hydrolysis ring
Section and fermenting step composition, wherein digesting link includes:
S1. the will be obtained by yak milk and porcine pancreatic lipase 3 ~ 8 h of mixed enzymolysis at 35 ~ 40 DEG C of filtration sterilization processing
One enzymolysis liquid；
S2. the first enzymolysis liquid is heated to 90 ~ 100 DEG C, 10 ~ 15 min is maintained to be inactivated；
S3. by the first enzymolysis liquid and protease 2 ~ 5 h of mixed enzymolysis at 38 ~ 43 DEG C after inactivation, the second enzymolysis liquid is obtained；
S4. the second enzymolysis liquid is heated to 90 ~ 100 DEG C, 10 ~ 15 min is maintained to be inactivated.
Yak is the main poultry kind of Qinghai-xizang Plateau Region, and the yak milk of production accounts for Qinghai-xizang Plateau Region milk consumption figure
90%.Compared to other milk, fat, protein, lactose, vitamin and essential amino acids content are higher in yak milk, fatty acid
Type is more, and cholesterol level is lower, is known as " superfine product in milk ".Studies have shown that the fat content in yak milk reach 6wt% ~
6.5wt%, protein content reach 4.8wt% ~ 5.3wt%.In contrast, the fat content of common milk cow's milk is only 4wt% or so,
Protein content is only 3.4wt% or so.Fat and protein are higher, cause the mouthfeel of yak milk to compare milk cow's milk difference larger,
It is more greasy, rougher to be embodied in mouthfeel.In view of the consumption habit of consumer and the tolerance level of stomach, existing yak
In dairy product production technology, its Oil content and Protein content can be all reduced by every means, and simplest method is exactly to add water
Dilution.But being diluted with water is the diluted process of entirety, not only fat and protein, the conjugation that people pay attention to the most are sub-
The content of oleic acid is equally reducing, its total amount is at this time just only supplied by additional conjugated linoleic acid.
In view of the above problem, present inventor has found after by a large amount of creative works, using the side of enzymatic hydrolysis
Partial fat and protein is hydrolyzed in formula, on the one hand controls the content of fat and protein in acceptable range
It is interior, it ensure that the mouthfeel of yak dairy products.On the other hand, the fat and protein being decomposed are converted to be easier to be inhaled by human body
Micromolecule polypeptide, fatty acid of receipts etc. ensure that its original nutritive value.
In addition to this, inventor also found, the conjugated linoleic acid in yak milk with free state other than existing, and there are also portions
Point be with it is existing after glycerine esterification, that is to say and exist in the form of fat.While being digested to fat, not but not
Because dilution causes cla levels to reduce, it can be also added because of new conjugated linoleic acid after fat enzymatic hydrolysis, content is into one
Step increases.In view of this, inventor has selected porcine pancreatic lipase to digest to the fat in yak milk.Porcine pancreatic lipase pair
There is specificity in long-chain, unsaturated fatty glyceride, it can length of the specific aim hydrolysis generation based on conjugated linoleic acid
Chain unsaturated fatty acid, to improve the whole content of conjugated linoleic acid in yak milk.
The embodiment of the present invention before digesting, is needing first to carry out to yak milk the processing such as filtration sterilization, and to yak
Milk Quality carries out considered critical.Specifically, the fresh high-quality cream of healthy dairy stock secretion is selected in selection.Naked eyes evaluation is in milky white
Color or micro-strip yellow: there is milk fragrance: in uniform colloidal state fluid: without precipitating, no grumeleuse, the impurity and sundries being visible by naked eyes
Deng, and must not use and inject cream secreted by the dairy stock of antibiotic at no distant date.Yak milk uses 200 mesh nylon mistakes after checking and accepting
Strainer and centrifugation milk clarifier remove mechanical admixture, surface microorganism, part body cell and gas in cow's milk.Measure acidity (ox
18 ° of T of milk) or alcohol testing (72 alcoholic strengths, do not generate flocculent deposit), then it is rapidly cooled to 0 ~ 6 DEG C.It is prevented in cooling procedure
Dust, impurity etc. enter Fresh Milk.After cooling by cow's milk be stored in through thorough disinfection, seal and be equipped with blender (revolving speed≤
In milk-storage tank 40r/min), it is desirable that newborn temperature must not exceed 6 DEG C.Penicillin and the test of other antibiotic are carried out when necessary.Then
It is strictly filtered and is purified, be allowed for access and digest link.
Further, in every 100 kg yak milk, the dosage of porcine pancreatic lipase is 5 ~ 10 DCU.In above-mentioned amount ranges
Interior, preferable to the hydrolysis effect of fat, hydrolysis degree is controllable.In hydrolytic process, tracking can be sampled, until the first enzymatic hydrolysis
The fat content of liquid reaches 4.5wt% ~ 5wt%.In the range, the taste good of yak dairy products.
Optionally, in every 100 kg yak milk, the dosage of protease is 2 ~ 6 DCU.In above-mentioned amount ranges, to albumen
The hydrolysis effect of matter is preferable, and hydrolysis degree is controllable.Protease is likewise supplied with the effect of hydrolysis to lipase, therefore, lipase and
Protease needs are added separately to.The application does not have specific requirement for protease, and decomposing protein is capable of in general food field
Protease can be used.Optionally, protease includes alkali protease, trypsase, papain and flavor protease
At least one of.In hydrolytic process, tracking can be sampled, until the protein content in the second enzymolysis liquid reaches
3.5wt%~4wt%.In the range, the taste good of yak dairy products.
The preparation method of high-purity conjugated linoleic acid yak yoghourt, fermenting step provided by the embodiment of the present invention include:
S5. the second enzymolysis liquid after inactivation is mixed with xylitol, emulsifier, by homogeneous, sterilization, obtains fermenation raw liquid；
S6. zymophyte is accessed in fermenation raw liquid to ferment.
Wherein, the mass ratio of yak milk, xylitol and emulsifier is 1:0.1 ~ 0.15:0.01 ~ 0.03.Emulsifier is for yak
Situations such as dairy product, mainly playing emulsification, it allowed to be not in layering, precipitating, while it is for conjugated linoleic acid
Also there is preferable stabilization.Optionally, emulsifier used by the embodiment of the present invention include monoglyceride, hydrophily monoglyceride and
Sucrose ester, wherein the mass ratio of monoglyceride, hydrophily monoglyceride and sucrose ester is 1:0.9 ~ 1.1:0.4 ~ 0.6.In aforementioned proportion
It is preferable for the stablizing effect of conjugated linoleic acid in range, the content of conjugated linoleic acid can be made preferably to be kept.
Further, it after the second enzymolysis liquid after inactivation being mixed with emulsifier, needs at 60 ~ 65 DEG C, 15 ~ 25 Mpa's
Under the conditions of carry out homogeneous, homogeneous can refine the lipochondrion in yak milk, improve the mouthfeel of yak milk.After homogeneous, heating
It sterilizes to 110 ~ 115 DEG C of 10 ~ 15 s of holding.
After sterilizing, after fermenation raw liquid is cooled to 42 ~ 45 DEG C, zymophyte is accessed.4 ~ 6 h are maintained at 42 ~ 45 DEG C, until
The pH of fermenation raw liquid reaches 4 ~ 4.2, and acidity reaches 80 ~ 110oT is subsequently moved to 10 ~ 15 h that ferment at 0 ~ 5 DEG C.Inventor is into one
Step discovery, during fermentation, the content of conjugated linoleic acid still can generate variation, select suitable zymogenic situation
Under, the content of conjugated linoleic acid can be there are also a degree of increase.Zymophyte includes bulgarian milk in embodiments of the present invention
At least one of bacillus, lactobacillus acidophilus, lactobacillus plantarum, in every 100 kg fermenation raw liquid, zymogenic dosage is 2 ~ 5
DCU.Preferably, the embodiment of the present invention is mixed according to mass ratio 1:0.8 ~ 1.2 in selection lactobacillus bulgaricus and lactobacillus plantarum
It is more obvious to the increase of cla levels when conjunction.
Feature and performance of the invention are described in further detail with reference to embodiments.
Embodiment 1
A kind of high-purity conjugated linoleic acid yak yoghourt is present embodiments provided, preparation method is as follows:
S1. by yak milk and porcine pancreatic lipase 8 h of mixed enzymolysis at 40 DEG C by filtration sterilization processing, the first enzyme is obtained
Solve liquid；Wherein, in every 100 kg yak milk, the dosage of porcine pancreatic lipase is 10 DCU.The fat content of first enzymolysis liquid is
4.58wt%。
S2. the first enzymolysis liquid is heated to 100 DEG C, 10 min is maintained to be inactivated.
S3. by the first enzymolysis liquid and protease 5 h of mixed enzymolysis at 40 DEG C after inactivation, the second enzymolysis liquid is obtained；Its
In, in every 100 kg yak milk, the dosage of protease is 6 DCU.Protease is alkali protease.Albumen in second enzymolysis liquid
Matter content is 3.54wt%.
S4. the second enzymolysis liquid is heated to 100 DEG C, 10 min is maintained to be inactivated.
S5. the second enzymolysis liquid after inactivation is mixed with xylitol, emulsifier, under conditions of 65 DEG C, 25 Mpa into
Row homogeneous.After homogeneous, it is warming up to 115 DEG C of 10 s of holding and sterilizes, obtain fermenation raw liquid；Wherein, yak milk, xylitol and
The mass ratio of emulsifier is 1:0.15:0.03.Emulsifier includes monoglyceride, hydrophily monoglyceride and sucrose ester, wherein single sweet
The mass ratio of ester, hydrophily monoglyceride and sucrose ester is 1:0.9:0.4.
S6. after fermenation raw liquid being cooled to 45 DEG C, zymophyte, the filling and sealing in 0.5 ~ 1 h are accessed, and move into fermentation
Constant heat storage maintains 4 h at 45 DEG C, until the pH of fermenation raw liquid reaches 4 ~ 4.2, acidity reaches 80 ~ 110oT.It is subsequently moved to 5 DEG C
10 h of lower fermentation.Wherein, zymophyte is lactobacillus bulgaricus, and in every 100 kg fermenation raw liquid, zymogenic dosage is 5
DCU。
Embodiment 2
A kind of high-purity conjugated linoleic acid yak yoghourt is present embodiments provided, preparation method is as follows:
S1. by yak milk and porcine pancreatic lipase 3 h of mixed enzymolysis at 35 DEG C by filtration sterilization processing, the first enzyme is obtained
Solve liquid；Wherein, in every 100 kg yak milk, the dosage of porcine pancreatic lipase is 5 DCU.The fat content of first enzymolysis liquid is 4.92
wt%。
S2. the first enzymolysis liquid is heated to 90 DEG C, 15 min is maintained to be inactivated.
S3. by the first enzymolysis liquid and protease 2 h of mixed enzymolysis at 43 DEG C after inactivation, the second enzymolysis liquid is obtained；Its
In, in every 100 kg yak milk, the dosage of protease is 2 DCU.Protease is trypsase.Protein in second enzymolysis liquid
Content is 3.89wt%.
S4. the second enzymolysis liquid is heated to 90 DEG C, 15 min is maintained to be inactivated.
S5. the second enzymolysis liquid after inactivation is mixed with xylitol, emulsifier, at 60 DEG C, is carried out under conditions of 15Mpa
Homogeneous.After homogeneous, it is warming up to 110 DEG C of 15 s of holding and sterilizes, obtain fermenation raw liquid；Wherein, yak milk, xylitol and cream
The mass ratio of agent is 1:0.1:0.01.Emulsifier includes monoglyceride, hydrophily monoglyceride and sucrose ester, wherein monoglyceride, parent
The mass ratio of aqueous monoglyceride and sucrose ester is 1:1:0.5.
S6. after fermenation raw liquid being cooled to 42 DEG C, zymophyte, the filling and sealing in 0.5 ~ 1 h are accessed, and move into fermentation
Constant heat storage maintains 6 h at 42 DEG C, until the pH of fermenation raw liquid reaches 4 ~ 4.2, acidity reaches 80 ~ 110oT.It is subsequently moved to 0 DEG C
15 h of lower fermentation.Wherein, zymophyte is lactobacillus bulgaricus and lactobacillus plantarum (1:1), in every 100 kg fermenation raw liquid, hair
The dosage of yeast-like fungi is 3 DCU.
Embodiment 3
A kind of high-purity conjugated linoleic acid yak yoghourt is present embodiments provided, preparation method is as follows:
S1. by yak milk and porcine pancreatic lipase 5 h of mixed enzymolysis at 37 DEG C by filtration sterilization processing, the first enzyme is obtained
Solve liquid；Wherein, in every 100 kg yak milk, the dosage of porcine pancreatic lipase is 8 DCU.The fat content of first enzymolysis liquid is 4.83
wt%。
S2. the first enzymolysis liquid is heated to 90 DEG C, 15 min is maintained to be inactivated.
S3. by the first enzymolysis liquid and protease 3 h of mixed enzymolysis at 38 DEG C after inactivation, the second enzymolysis liquid is obtained；Its
In, in every 100 kg yak milk, the dosage of protease is 4 DCU.Protease is papain and flavor protease (1:1).
Protein content in second enzymolysis liquid is 3.67wt%.
S4. the second enzymolysis liquid is heated to 90 DEG C, 15 min is maintained to be inactivated.
S5. the second enzymolysis liquid after inactivation is mixed with xylitol, emulsifier, under conditions of 65 DEG C, 25 Mpa into
Row homogeneous.After homogeneous, it is warming up to 110 DEG C of 15 s of holding and sterilizes, obtain fermenation raw liquid；Wherein, yak milk, xylitol and
The mass ratio of emulsifier is 1:0.12:0.02.Emulsifier includes monoglyceride, hydrophily monoglyceride and sucrose ester, wherein single sweet
The mass ratio of ester, hydrophily monoglyceride and sucrose ester is 1:1:0.6.
S6. after fermenation raw liquid being cooled to 42 DEG C, zymophyte, the filling and sealing in 0.5 ~ 1 h are accessed, and move into fermentation
Constant heat storage maintains 6 h at 42 DEG C, until the pH of fermenation raw liquid reaches 4 ~ 4.2, acidity reaches 80 ~ 110oT.It is subsequently moved to 5 DEG C
12 h of lower fermentation.Wherein, zymophyte is lactobacillus acidophilus, and in every 100 kg fermenation raw liquid, zymogenic dosage is 5 DCU.
Comparative example 1
This comparative example provides a kind of high-purity conjugated linoleic acid yak yoghourt, and preparation method is as follows:
S1. it will be diluted with water by the yak milk of filtration sterilization processing, until fat content and protein content and milk cow's milk phase
When.
S2. the yak milk after dilution is mixed with xylitol, emulsifier, at 60 DEG C, homogeneous is carried out under conditions of 15Mpa.
After homogeneous, it is warming up to 110 DEG C of 15 s of holding and sterilizes, obtain fermenation raw liquid；Wherein, yak milk, xylitol and emulsifier
Mass ratio be 1:0.1:0.01.Emulsifier includes monoglyceride, hydrophily monoglyceride and sucrose ester, wherein monoglyceride, hydrophily
The mass ratio of monoglyceride and sucrose ester is 1:1:0.5.
S3. after fermenation raw liquid being cooled to 42 DEG C, zymophyte, the filling and sealing in 0.5 ~ 1 h are accessed, and move into fermentation
Constant heat storage maintains 6 h at 42 DEG C, until the pH of fermenation raw liquid reaches 4 ~ 4.2, acidity reaches 80 ~ 110oT.It is subsequently moved to 0 DEG C
15 h of lower fermentation.Wherein, zymophyte is lactobacillus bulgaricus and lactobacillus plantarum (1:1), in every 100 kg fermenation raw liquid, hair
The dosage of yeast-like fungi is 3 DCU.
Comparative example 2
This comparative example provides a kind of high-purity conjugated linoleic acid yak yoghourt, and preparation method is as follows:
S1. by the yak milk and CCL lipase 3 h of mixed enzymolysis at 35 DEG C by filtration sterilization processing, the first enzymatic hydrolysis is obtained
Liquid；Wherein, in every 100 kg yak milk, the dosage of CCL lipase is 5 DCU.The fat content of first enzymolysis liquid is 4.85
wt%。
S2. the first enzymolysis liquid is heated to 90 DEG C, 15 min is maintained to be inactivated.
S3. by the first enzymolysis liquid and protease 2 h of mixed enzymolysis at 43 DEG C after inactivation, the second enzymolysis liquid is obtained；Its
In, in every 100 kg yak milk, the dosage of protease is 2 DCU.Protease is trypsase.Protein in second enzymolysis liquid
Content is 3.84wt%.
S4. the second enzymolysis liquid is heated to 90 DEG C, 15 min is maintained to be inactivated.
S5. the second enzymolysis liquid after inactivation is mixed with xylitol, emulsifier, at 60 DEG C, is carried out under conditions of 15Mpa
Homogeneous.After homogeneous, it is warming up to 110 DEG C of 15 s of holding and sterilizes, obtain fermenation raw liquid；Wherein, yak milk, xylitol and cream
The mass ratio of agent is 1:0.1:0.01.Emulsifier includes monoglyceride, hydrophily monoglyceride and sucrose ester, wherein monoglyceride, parent
The mass ratio of aqueous monoglyceride and sucrose ester is 1:1:0.5.
S6. after fermenation raw liquid being cooled to 42 DEG C, zymophyte, the filling and sealing in 0.5 ~ 1 h are accessed, and move into fermentation
Constant heat storage maintains 6 h at 42 DEG C, until the pH of fermenation raw liquid reaches 4 ~ 4.2, acidity reaches 80 ~ 110oT.It is subsequently moved to 0 DEG C
15 h of lower fermentation.Wherein, zymophyte is lactobacillus bulgaricus and lactobacillus plantarum (1:1), in every 100 kg fermenation raw liquid, hair
The dosage of yeast-like fungi is 3 DCU.
Test example
Prepared by the preparation method of high-purity conjugated linoleic acid yak yoghourt of selection example 1 ~ 3 and comparative example 1, each
The different stages are sampled, and are tested protein, fat, cla levels in the sample of acquisition, test result
As shown in table 1.Wherein, in order to preferably compare, used yak milk is same batch, is surveyed its protein content and is
6.21 wt%, fat content are 5.14 wt%, 0.586 mg/ml of cla levels.
Each stage constituent analysis of table 1.
As can be seen from Table 1, the preparation method of the embodiment of the present invention 1 ~ 3 exists protein, fat content control completing enzymatic hydrolysis
While tolerance interval, the content of conjugated linoleic acid has apparent increase, has improved 2 ~ 3 times, has shown in enzymolysis process
In, conjugated linoleic acid is additionally generated really.In contrast, comparative example 1 controlled using traditional dilution mode protein and
Fat content results in being substantially reduced for cla levels.Comparative example 2 is replaced using the CCL lipase for not having specificity
It is not obvious for the porcine pancreatic lipase of the application although conjugated linoleic acid also has certain increase.And Yoghourt is prepared in fermentation
During, either there is a degree of reduction in embodiment 1 ~ 3 or comparative example 1 ~ 2, protein and fat content, can
To speculate in fermentation process, there are also a degree of hydrolysis for protein and fat, but are not obvious.Corresponding is the sub- oil of conjugation
Acid content have a degree of increase, especially embodiment 2 after optimizing zymophyte, conjugated linoleic acid relative increase compared with
It is more.
In conclusion the embodiment of the invention provides a kind of preparation method of high-purity conjugated linoleic acid yak yoghourt, elder generation
To in yak milk fat and protein digest, be translated into the small molecule compound more conducively absorbed, then to enzymatic hydrolysis
Liquid carries out fermentation operation.Such enzymatic hydrolysis includes two effects.First, the more conventional ox of Oil content and Protein content in yak milk
Milk is higher, in order to guarantee its mouthfeel, needs to be reduced to and the comparable level of conventional milk；Second, the present invention is to fat
When enzyme is digested, porcine pancreatic lipase is selected to carry out, porcine pancreatic lipase has the fat containing unsaturated linoleic acid structure
Specificity increases whole cla levels to release conjugated linoleic acid from lipid structure.The preparation method
It is easy to operate, it is not high to equipment requirement, it is appropriate for industrialization promotion.
The embodiment of the invention also provides a kind of high-purity conjugated linoleic acid yak yoghourts, are prepared by above-mentioned preparation method
It arrives.After study, test prove, high-purity conjugated linoleic acid yak yoghourt have button cancer, weight-reducing, adjust blood lipid, adjust blood glucose,
It adjusts blood pressure, antiatherosclerosis, immunological regulation, improve a variety of important physiological functions such as bone density, there is no so-called
SC service ceiling is not present in homology problem in principle, often edible, has no toxic side effect, can play important work to human health
With.It is required according to the thought of inventor, which can satisfy people's demand growing to healthy food, reach inventor
Goal of the invention.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of preparation method of high-purity conjugated linoleic acid yak yoghourt characterized by comprising
By yak milk and porcine pancreatic lipase 3 ~ 8 h of mixed enzymolysis at 35 ~ 40 DEG C by filtration sterilization processing, the first enzyme is obtained
Solve liquid；
First enzymolysis liquid is heated to 90 ~ 100 DEG C, 10 ~ 15 min is maintained to be inactivated；
By first enzymolysis liquid and protease 2 ~ 5 h of mixed enzymolysis at 38 ~ 43 DEG C after inactivation, the second enzymolysis liquid is obtained；
Second enzymolysis liquid is heated to 90 ~ 100 DEG C, 10 ~ 15 min is maintained to be inactivated；
Second enzymolysis liquid after inactivation is mixed with xylitol, emulsifier, by homogeneous, sterilization, obtains fermenation raw liquid；
Zymophyte is accessed in the fermenation raw liquid to ferment.
2. the preparation method of high-purity conjugated linoleic acid yak yoghourt according to claim 1, which is characterized in that every 100 kg
In the yak milk, the dosage of the porcine pancreatic lipase is 5 ~ 10 DCU.
3. the preparation method of high-purity conjugated linoleic acid yak yoghourt according to claim 2, which is characterized in that described first
The fat content of enzymolysis liquid is 4.5wt% ~ 5wt%.
4. the preparation method of high-purity conjugated linoleic acid yak yoghourt according to claim 1, which is characterized in that every 100 kg
In the yak milk, the dosage of the protease is 2 ~ 6 DCU；Preferably, the protease includes alkali protease, tryptose
At least one of enzyme, papain and flavor protease.
5. the preparation method of high-purity conjugated linoleic acid yak yoghourt according to claim 4, which is characterized in that described second
Protein content in enzymolysis liquid is 3.5wt% ~ 4wt%.
6. the preparation method of high-purity conjugated linoleic acid yak yoghourt according to claim 1, which is characterized in that the yak
The mass ratio of newborn, the described xylitol and the emulsifier is 1:0.1 ~ 0.15:0.01 ~ 0.03；Preferably, the emulsifier includes
The mass ratio of monoglyceride, hydrophily monoglyceride and sucrose ester, the monoglyceride, the hydrophily monoglyceride and the sucrose ester is
1:0.9 ~ 1.1:0.4 ~ 0.6.
7. the preparation method of high-purity conjugated linoleic acid yak yoghourt according to claim 1, which is characterized in that after inactivation
Second enzymolysis liquid mixed with the emulsifier after, homogeneous is carried out under conditions of 60 ~ 65 DEG C, 15 ~ 25 Mpa, and heat up
It sterilizes to 110 ~ 115 DEG C of 10 ~ 15 s of holding.
8. the preparation method of high-purity conjugated linoleic acid yak yoghourt according to claim 7, which is characterized in that by the hair
After ferment stoste is cooled to 42 ~ 45 DEG C, the zymophyte is accessed；4 ~ 6 h are maintained at 42 ~ 45 DEG C, until the fermenation raw liquid
PH reaches 4 ~ 4.2, and acidity reaches 80 ~ 110oT；It is subsequently moved to 10 ~ 15 h that ferment at 0 ~ 5 DEG C.
9. the preparation method of high-purity conjugated linoleic acid yak yoghourt according to claim 8, which is characterized in that the fermentation
Bacterium bag includes at least one of lactobacillus bulgaricus, lactobacillus acidophilus, lactobacillus plantarum；Preferably, it is sent out described in every 100 kg
In ferment stoste, the zymogenic dosage is 2 ~ 5 DCU.
10. a kind of high-purity conjugated linoleic acid yak yoghourt, which is characterized in that described in any item high-purity total by claim 1 ~ 9
The preparation method of conjugated linoleic acid yak yoghourt is prepared.
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