CN110331088A - Microlayer model fluorescence detecting system and method - Google Patents
Microlayer model fluorescence detecting system and method Download PDFInfo
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- CN110331088A CN110331088A CN201910761119.3A CN201910761119A CN110331088A CN 110331088 A CN110331088 A CN 110331088A CN 201910761119 A CN201910761119 A CN 201910761119A CN 110331088 A CN110331088 A CN 110331088A
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Abstract
The present invention provides a kind of microlayer model fluorescence detecting system and method, one of microlayer model fluorescence detecting system, it include: sample holding unit, with the sample storage portion for storing sample to be tested and for forming carvel-built tiling channel to sample to be tested, sample storage portion is connect with tiling communication conduits；Optical image unit is correspondingly arranged with tiling channel, for carrying out optical imagery to the sample to be tested in tiling channel；Fluid driving unit has sample introduction agent driving portion, and perforation may be selected in sample introduction agent driving portion and sample storage portion, for being transferred to sample to be tested in tiling channel by sample storage portion by the sample introduction agent in sample introduction agent driving portion.A kind of microlayer model fluorescence detecting system of the invention and method avoid limitation of the chip area to ddPCR technical application in the prior art, can be realized high-throughput microlayer model detection by carrying out optical imagery in batches to microlayer model.
Description
Technical field
The present invention relates to microfluidic arts, and in particular to a kind of microlayer model fluorescence detecting system and method.
Background technique
Microlayer model formula digital pcr (droplet digital PCR, ddPCR) is a kind of round pcr emerging in recent years.
In the former technical foundation with PCR, which is significantly reformed.Compared with classical quantitative fluorescent PCR, ddPCR can
It is analyzed in a manner of using absolute quantitation to DNA or RNA molecule, does not need standard curve assistant analysis.The technology will
Every a example reaction system in normal PCR is divided into several pieces, then sample carries out again only by package in microlayer model
PCR reaction.PCR after reaction, the fluorescence signal value of each microlayer model is read using detection device, further according to fluorescence signal
The threshold value of value distinguishes each drop, and the microlayer model that fluorescence signal is higher than threshold value is known as positive drop, fluorescence signal is lower than threshold
The microlayer model of value becomes negative drop, according to Poisson distribution principle and the number and ratio of negative drop, calculates sample target
The starting copy number of molecule.The technology and it is widely used in the discovery of Cancer Molecular marker, infectious disease research, gene knot
The fields such as structure analysis of variance, gene expression analysis.
When carrying out fluorescence detection to the microlayer model after PCR, imaging method can be usually used, it will be to be checked using microlayer model chip
Sample drop realizes that single layer tiles in the chip, carries out fluorescence imaging to it followed by CCD or CMOS and analyzes its yin
It is positive.The quantity of microlayer model is directly proportional to the required area that tiles in the chip in sample, although more microlayer model numbers can increase
The detection range of big ddPCR technology, but it is also required to bigger chip area simultaneously.In practical applications, in a sample often
Containing tens of thousands of or even millions of a microlayer models, since chip is by precision machined, chip area suffers from limitation, causes
Limit the number of drops of sample detection.Based on limitation above-mentioned, the existing detection system using imaging method often can only be to reality
Now the number of drops lower than 30,000 detects, and limits the application scenarios of ddPCR technology.
Summary of the invention
Therefore, the technical problem to be solved in the present invention is that providing a kind of microlayer model fluorescence detecting system and method, pass through
Optical imagery is carried out to microlayer model in batches, avoids limitation of the chip area to ddPCR technical application in the prior art, it can
Realize high-throughput microlayer model detection.
To solve the above-mentioned problems, the present invention provides a kind of microlayer model fluorescence detecting system, the system comprises: sample is protected
Unit is held, there is the sample storage portion of storage sample to be tested and is led to for forming carvel-built tiling to sample to be tested
Road, the sample storage portion are connect with the tiling communication conduits；Optical image unit is correspondingly arranged with the tiling channel,
For carrying out optical imagery to the sample to be tested in the tiling channel；Fluid driving unit has sample introduction agent driving
Perforation may be selected in portion, the sample introduction agent driving portion and the sample storage portion, for by the sample introduction agent driving portion into
The sample to be tested is transferred in the tiling channel by sample agent by the sample storage portion.
Preferably, the sample holding unit further includes waste liquid storage unit, and the waste liquid storage unit is logical by the tiling
Road is connect with sample storage portion pipeline.
Preferably, the fluid driving unit further includes cleaning agent driving portion, the cleaning agent driving portion and the tiling
Communication conduits connection, to drive the cleaning agent in the cleaning agent driving portion to clean the test sample to be checked in the tiling channel
Product.
Preferably, the sample introduction agent driving portion includes syringe pump；And/or the cleaning agent driving portion includes syringe pump.
Preferably, the height in the tiling channel is equal with the diameter of microlayer model in the sample to be tested.
Preferably, the material of the sample holding unit is polycarbonate；And/or the tiling channel is towards the light
The material for learning the side side wall of imaging unit is translucent material；And/or the tiling channel is far from the optical image unit
There is reflecting material on the side wall of side.
Preferably, the optical imagery includes light field imaging, and the optical image unit includes image-forming block, wide spectrum optical
Source, the wide spectrum light source are used to provide white-light illuminating for light field imaging.
Preferably, the optical imagery further includes glimmering optical field imaging, and the optical image unit further includes filter set, is used
In the fluorescence of the exciting light and reception specific band that emit specific band when carrying out glimmering optical field imaging.
Preferably, the image-forming block includes CMOS camera.
The present invention also provides a kind of microlayer model fluorescence detection methods, are carried out using above-mentioned microlayer model fluorescence detecting system,
Include the following steps:
First test sample tiling step, the sample introduction agent driving portion operating controlled in fluid driving unit make sample storage
First test sample in sample to be tested in portion enters tiling channel, when first described test sample single layer is laid in
When in the tiling channel, controls the sample introduction agent driving portion and shut down；
Optical imaging step, starting optical image unit is to first test sample described in the tiling channel
It is detected；
Cleaning step, control the cleaning agent driving portion operating in fluid driving unit clean first described test sample into
Enter waste liquid storage unit, when first described test sample is completely into the waste liquid storage unit, control cleaning agent driving portion stops
Only operate；
The tiling of first test sample step, optical imaging step, cleaning step are repeated, until the sample storage
Sample to be tested in portion is fully completed detection.
A kind of microlayer model fluorescence detecting system provided by the invention and method, can be to be checked in the sample storage portion
The tiling of carry out single layer, the detection operation of sample in batches, without the excessive to be checked to meet of the area processing to chip
Sample namely avoids limitation of the chip area to ddPCR technical application in the prior art to the high demand of tiling area, leads to
Mode after sample introduction, tiling detection in batches realizes high-throughput microlayer model detection；In addition, due to such mode high degree
The demand reduced with chip area, thus the design cost for reducing corresponding system of high degree.
Detailed description of the invention
Fig. 1 is the schematic diagram of the microlayer model fluorescence detecting system of the embodiment of the present invention；
The status diagram that Fig. 2 penetrates through for sample introduction agent driving portion in the fluid driving unit in Fig. 1 with sample storage portion；
The status diagram that Fig. 3 penetrates through for cleaning agent driving portion in the fluid driving unit in Fig. 1 with tiling channel；
Fig. 4 is the light field images obtained using microlayer model fluorescence detecting system of the invention；
Fig. 5 is the fluorescence field images obtained using microlayer model fluorescence detecting system of the invention.
Appended drawing reference are as follows:
1, sample holding unit；11, sample storage portion；12, tiling channel；13, waste liquid storage unit；14, first passage；
15, second channel；2, optical image unit；3, fluid driving unit；100, sample to be tested.
Specific embodiment
In conjunction with referring to figs. 1 to 5, according to an embodiment of the invention, providing a kind of microlayer model fluorescence detecting system, wrap
Include: sample holding unit 1 has the sample storage portion 11 of storage sample to be tested 100 and for 100 shape of sample to be tested
At carvel-built tiling channel 12, the sample storage portion 11 is connect with 12 pipeline of tiling channel；Optical image unit
2, it is correspondingly arranged with the tiling channel 12, for carrying out optics to the sample to be tested 100 in the tiling channel 12
Imaging；Fluid driving unit 3, has sample introduction agent driving portion, and the sample introduction agent driving portion and the sample storage portion 11 may be selected
Perforation, for by the sample introduction agent in the sample introduction agent driving portion by the sample to be tested 100 by the sample storage portion 11
It is transferred in the tiling channel 12, in a kind of specific embodiment, such as the sample introduction agent is liquid, specifically can be with
For fluoroalkane hydrocarbon chain, and the sample to be tested 100 is then Water-In-Oil drop, oil-phase component therein and the sample introduction agent at
Split-phase is same, therein when the sample introduction agent enters in the sample storage portion 11 under the action of the sample introduction agent driving portion
The sample to be tested 100 floats up under the action of the sample introduction agent, and finally floating to it is described tiling channel 12 height,
Sample to be tested 100 enters in the tiling channel 12 and is formed single layer tiling, and the optical image unit 2 is to shape at this time
At the carvel-built sample to be tested 100 detected, after detection, by it is described tiling channel 12 in detection
The sample finished removes (such as can be by the way of being passed through high pressure compressed gas), and then sample introduction agent described in secondary control drives again
Portion operates and realizes floating, tiling, detection, the process removed as the aforementioned, until by the whole in the sample storage portion 11
All detection finishes for sample to be tested 100, to realize realizing and detect to sample to be tested 100 in batches.It can be seen that adopting
It, can be to the carry out single flat of the sample to be tested 100 in the sample storage portion 11 in batches with technical solution in the present invention
Paving, detection operation, without to chip area processing it is excessive come meet sample to be tested to tiling area high demand,
Namely limitation of the chip area to ddPCR technical application in the prior art is avoided, it is detected by sample introduction in batches, tiling
Mode realizes high-throughput microlayer model detection；In addition, due to the demand of such mode high degree reduced with chip area,
To the design cost for reducing corresponding system of high degree.Sample introduction agent driving portion above-mentioned can for example pass through first passage
14, which form pipeline with the sample storage portion 11, connect (such as Fig. 2), and as a kind of specific embodiment, the sample is deposited
Storage portion 11 can be 10mm for a diameter, the hydrostatic column of a height of 10mm is formed, and its top is then configured with outlet, described
Outlet is then penetrated through with the tiling channel 12 and is connected.
Preferably, the sample holding unit 1 further includes waste liquid storage unit 13, and the waste liquid storage unit 13 passes through described flat
12 were laid to connect with 11 pipeline of sample storage portion, and it is understood that the waste liquid storage unit 13, tiling channel 12
And the sample storage portion 11 is integrally molded so as an entirety, keeps the structure of the sample holding unit 1 more compact, example
Such as, the material of the sample holding unit 1 is polycarbonate, namely all makes to be formed using translucent material, and/or, it is described
The material of tiling channel 12 towards the side side wall of the optical image unit 2 is translucent material, is prevented to the optical imagery
Unit 2 adversely affects；Further, on the side side wall of the tiling channel 12 far from the optical image unit 2
With reflecting material.As a kind of specific embodiment of the waste liquid storage unit 13, for example, the waste liquid storage unit 13 is one
A diameter is that the volume of 20mm, the cylindrical container of a height of 20mm namely the waste liquid storage unit should be greater than the sample storage
The volume in portion 11.Totally enclosed sample detection, detection process are realized using the sample holding unit 1 in the technical solution
It completes, and is finally collected in waste liquid storage unit 13 in closed sample holding unit 1, avoid nucleic acids in samples product
Pollution to environment.
As previously mentioned, having been detected every time per needing it after a batch of sample to be tested 100 from the tiling channel
It is removed in 12, and reset mode can be multiplicity, as a preferred embodiment, the fluid driving unit 3 also wraps
Cleaning agent driving portion is included, the cleaning agent driving portion is connect with 12 pipeline of tiling channel, specifically, the cleaning agent drives
Portion forms pipeline by second channel 15 with the tiling channel 12 and connect (such as Fig. 3), to drive in the cleaning agent driving portion
Cleaning agent clean it is described tiling channel 12 in the sample to be tested 100, the cleaning agent can be with institute at division aspect
It is consistent to state sample introduction agent selection.
The sample introduction agent driving portion includes syringe pump (can also be peristaltic pump)；And/or the cleaning agent driving portion includes note
It penetrates pump (can also be peristaltic pump), the syringe pump is commercially available part, this can reduce the design of the detection system, manufacturing cost.
As a kind of specific embodiment in the tiling channel 12, the tiling channel 12 can be a rectangular-shape
Cavity, in terms of size, length and width can flexibly be set according to operation purpose, and in terms of height, it is preferable that the tiling
The height in channel 12 is equal with the diameter of microlayer model in the sample to be tested 100, for example, the length in the tiling channel 12 is
30mm, width 20mm, when the diameter of microlayer model is 0.1mm, its height is also 0.1mm, at this point, into the tiling channel
The sample to be tested 100 in 12 can be by more convenient formation single layer tiling effects.
The optical imagery include light field imaging and glimmering optical field imaging in any one, the two is on optical component
Difference is whether to remove corresponding filter set.Specifically, when the optical imagery is that light field is imaged, the optical imagery list
Member 2 includes image-forming block, wide spectrum light source, and the wide spectrum light source is used to provide white-light illuminating for light field imaging；When the optics at
When as including glimmering optical field imaging, the optical image unit 2 further includes filter set, for emitting when carrying out glimmering optical field imaging
The exciting light of specific band and the fluorescence for receiving specific band.Preferably, CMOS camera, resolution ratio can be used in the image-forming block
It is 6000 × 4000, Pixel Dimensions are 5 μm of 5 μ m, and cooperation diameter is 100mm, the object lens that amplification factor is 1 times.Wide spectrum light source
The LED white light source for being 10W for power provides white-light illuminating for light field imaging, while when being intended to cooperate filter set, additionally it is possible to
Exciting light is provided for fluorescence imaging.Filter set includes two tablet filters, and exciting light optical filter uses bandwidth 10nm, central wavelength
For the bandpass filter of 473nm, it is placed in wide spectrum light source transmitting terminal, the light that central wavelength is 473nm is only allowed to penetrate；Fluorescence filter
Mating plate uses bandwidth 20nm, central wavelength as the bandpass filter of 520nm, is placed in front of the object lens of imaging unit, only allows
The fluorescence that central wavelength is 520nm penetrates.When imaging optical path carries out light field imaging, filter set is removed, and is not involved in light field
Work.When imaging optical path carries out glimmering optical field imaging, filter set switches in optical path, emits exciting light and the reception of specific band
The fluorescence of specific band.
The present invention also provides a kind of microlayer model fluorescence detection methods, are carried out using above-mentioned microlayer model fluorescence detecting system,
Include the following steps:
First test sample tiling step, the sample introduction agent driving portion operating controlled in fluid driving unit 3 deposit sample
First test sample in sample to be tested 100 in storage portion 11 enters tiling channel 12, when first described test sample
When single layer is laid in the tiling channel 12, controls the sample introduction agent driving portion and shut down；
Optical imaging step, starting optical image unit 2 to described in the tiling channel 12 first detect
Sample is detected, and Fig. 4 or photo illustrated in fig. 5 can be obtained；
First described test sample is cleaned in cleaning step, the cleaning agent driving portion operating controlled in fluid driving unit 3
Into waste liquid storage unit 13, when first described test sample is completely into the waste liquid storage unit 13, control cleaning agent is driven
Dynamic portion shuts down；
The tiling of first test sample step, optical imaging step, cleaning step are repeated, until the sample storage
Sample to be tested 100 in portion 11 is fully completed detection.
Those skilled in the art will readily recognize that above-mentioned each advantageous manner can be free under the premise of not conflicting
Ground combination, superposition.
The above is merely preferred embodiments of the present invention, be not intended to limit the invention, it is all in spirit of the invention and
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within principle.Above only
It is the preferred embodiment of the present invention, it is noted that for those skilled in the art, do not departing from this hair
Under the premise of bright technical principle, several improvements and modifications can also be made, these improvements and modifications also should be regarded as guarantor of the invention
Protect range.
Claims (10)
1. microlayer model fluorescence detecting system, which is characterized in that the system comprises:
Sample holding unit (1) has the sample storage portion (11) of storage sample to be tested (100) and for sample to be detected
Product (100) form carvel-built tiling channel (12), and the sample storage portion (11) and the tiling channel (12) pipeline connect
It connects；
Optical image unit (2) is correspondingly arranged with the tiling channel (12), for in the tiling channel (12)
Sample to be tested (100) carries out optical imagery；
Fluid driving unit (3) has sample introduction agent driving portion, and the sample introduction agent driving portion and the sample storage portion (11) are optional
Select perforation, for by the sample introduction agent in the sample introduction agent driving portion by the sample to be tested (100) by the sample storage
Portion (11) is transferred in the tiling channel (12).
2. detection system according to claim 1, which is characterized in that the sample holding unit (1) further includes that waste liquid is deposited
Storage portion (13), the waste liquid storage unit (13) are connect by the tiling channel (12) with sample storage portion (11) pipeline.
3. detection system according to claim 2, which is characterized in that
The fluid driving unit (3) further includes cleaning agent driving portion, the cleaning agent driving portion and the tiling channel (12)
Pipeline connection, to drive the cleaning agent in the cleaning agent driving portion to clean the test sample to be checked in tiling channel (12)
Product (100).
4. detection system according to claim 3, which is characterized in that
The sample introduction agent driving portion includes syringe pump；And/or the cleaning agent driving portion includes syringe pump.
5. detection system according to claim 1, which is characterized in that
The height of tiling channel (12) is equal with the diameter of microlayer model in the sample to be tested (100).
6. detection system according to claim 1, which is characterized in that the material of the sample holding unit (1) is poly- carbon
Acid esters；And/or the material of side side wall of tiling channel (12) towards the optical image unit (2) is translucent material；
And/or there is reflecting material on the side side wall of tiling channel (12) separate the optical image unit (2).
7. detection system according to claim 1, which is characterized in that the optical imagery includes light field imaging, the light
Learning imaging unit (2) includes image-forming block, wide spectrum light source, and the wide spectrum light source is used to provide white-light illuminating for light field imaging.
8. detection system according to claim 7, which is characterized in that the optical imagery further includes glimmering optical field imaging, institute
Stating optical image unit (2) further includes filter set, for when carrying out glimmering optical field imaging emit specific band exciting light and
Receive the fluorescence of specific band.
9. detection system according to claim 7, which is characterized in that the image-forming block includes CMOS camera.
10. a kind of microlayer model fluorescence detection method, which is characterized in that using microlayer model described in any one of claims 1 to 9
Fluorescence detecting system carries out, and includes the following steps:
First test sample tiling step, the sample introduction agent driving portion operating controlled in fluid driving unit (3) make sample storage
First test sample in sample to be tested (100) in portion (11) enters tiling channel (12), when first described detection
When sample single layer is laid in the tiling channel (12), controls the sample introduction agent driving portion and shut down；
Optical imaging step, starting optical image unit (2) is to first described detection in tiling channel (12)
Sample is detected；
Cleaning step, the cleaning agent driving portion operating in control fluid driving unit (3) clean first described test sample into
Enter waste liquid storage unit (13), when first described test sample is completely into waste liquid storage unit (13), controls cleaning agent
Driving portion shuts down；
The tiling of first test sample step, optical imaging step, cleaning step are repeated, until the sample storage portion
(11) sample to be tested (100) in is fully completed detection.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140141991A1 (en) * | 2012-11-09 | 2014-05-22 | Arizona Board of Regents, a body Corporate of the State of Arizona, Acting for and on Behalf of Ariz | High throughput detection of fusion proteins |
| CN106442443A (en) * | 2016-09-12 | 2017-02-22 | 清华大学 | Micro-droplet fluorescence detection system |
| CN107478629A (en) * | 2017-09-04 | 2017-12-15 | 中国科学院苏州生物医学工程技术研究所 | A kind of large area digital pcr droplet fluorescence high pass amount detecting device and method |
| CN109746062A (en) * | 2017-11-06 | 2019-05-14 | 北京新羿生物科技有限公司 | Microlayer model generating means |
| CN210656937U (en) * | 2019-08-17 | 2020-06-02 | 新羿制造科技（北京）有限公司 | Micro-droplet fluorescence detection system |
-
2019
- 2019-08-17 CN CN201910761119.3A patent/CN110331088B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140141991A1 (en) * | 2012-11-09 | 2014-05-22 | Arizona Board of Regents, a body Corporate of the State of Arizona, Acting for and on Behalf of Ariz | High throughput detection of fusion proteins |
| CN106442443A (en) * | 2016-09-12 | 2017-02-22 | 清华大学 | Micro-droplet fluorescence detection system |
| CN107478629A (en) * | 2017-09-04 | 2017-12-15 | 中国科学院苏州生物医学工程技术研究所 | A kind of large area digital pcr droplet fluorescence high pass amount detecting device and method |
| CN109746062A (en) * | 2017-11-06 | 2019-05-14 | 北京新羿生物科技有限公司 | Microlayer model generating means |
| CN210656937U (en) * | 2019-08-17 | 2020-06-02 | 新羿制造科技（北京）有限公司 | Micro-droplet fluorescence detection system |
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