CN110330573A - A method of low molecular weight Blackfungus polyhexose is prepared using ultrasonic technology - Google Patents
A method of low molecular weight Blackfungus polyhexose is prepared using ultrasonic technology Download PDFInfo
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- CN110330573A CN110330573A CN201910560943.2A CN201910560943A CN110330573A CN 110330573 A CN110330573 A CN 110330573A CN 201910560943 A CN201910560943 A CN 201910560943A CN 110330573 A CN110330573 A CN 110330573A
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
A method of low molecular weight Blackfungus polyhexose being prepared using ultrasonic technology, is related to polysaccharide preparation technical field.The present invention solves the problem of existing Blackfungus polyhexose molecular weight is excessive, and inherent viscosity is higher, is unfavorable for application.The method is as follows: preparing the Blackfungus polyhexose aqueous solution of certain mass score；Prepared Blackfungus polyhexose aqueous solution is placed in water-bath, ultrasonic probe is inserted into Blackfungus polyhexose aqueous solution and carries out ultrasound, after ultrasonication, Blackfungus polyhexose aqueous solution is placed in bag filter, it is placed in distilled water and dialyses, after vacuum freeze-drying, obtain low molecular weight Blackfungus polyhexose.Low molecular weight Blackfungus polyhexose prepared by the present invention has lower viscosity average molecular weigh and inherent viscosity, and solubility is preferable in water, while having fine antioxidant activity, shows that it can be used as better anti-oxidant, anti-aging, anti-myocardial anoxia and anti-rad.It can be used as the functional food or drug for improving body physiological function.
Description
Technical field
The invention belongs to polysaccharide preparation technical fields, and in particular to a kind of to prepare the black wood of low molecular weight using ultrasonic technology
The method of fungus polysaccharides.
Background technique
Black fungus (AuriculariaAuricular) is also known as agaric, the ears or side handles of a utensil, brightness program, belongs to Eumycota Basidiomycetes agaric
Section's Auricularia mushroom.China is that main cultivation of auricularia auricula state, annual output account for 90% of Gross World Product or more in the world, this is just
Advantage is provided for the development and application of black fungus.Wherein Blackfungus polyhexose is as its main active, domestic and foreign literature
Report that black fungus fruitbody polysaccharide has anti-oxidant, reducing blood lipid, hypoglycemic, antitumor, anticoagulation, anti-aging and improves myocardium function
It can isoreactivity.But the Blackfungus polyhexose molecular weight of research report usually reaches 2.05 × 10 at present4~5 × 106Da, while its
Inherent viscosity is higher, significantly limits Blackfungus polyhexose in the application of food and medicine field, while it is more also to influence black fungus
Sugar body metabolism, by the end of the rare report of research of the current Blackfungus polyhexose in relation to low molecular weight.
Summary of the invention
The purpose of the present invention is to solve existing Blackfungus polyhexoses because molecular weight is big and inherent viscosity is high, strongly limits
Blackfungus polyhexose is provided and a kind of prepares the black wood of low molecular weight using ultrasonic technology the application of food and medicine field the problem of
The method of fungus polysaccharides.
To achieve the above object, the technical solution adopted by the present invention is as follows:
A method of low molecular weight Blackfungus polyhexose being prepared using ultrasonic technology, the method comprises the following steps:
Step 1: preparing Blackfungus polyhexose aqueous solution: weighing the Blackfungus polyhexose of certain mass, distilled water is added, by it
It is configured to the Blackfungus polyhexose aqueous solution that mass fraction is 1%~20%；
Step 2: prepared Blackfungus polyhexose aqueous solution is placed in water-bath, bath temperature is 0~100 DEG C；
Step 3: ultrasonic probe is inserted into Blackfungus polyhexose aqueous solution, insertion depth is 1~10cm；
Step 4: the power of adjustment ultrasonic wave is 200~1000W, the processing time is 1~12h；
Step 5: it is 500~3000Da that Blackfungus polyhexose aqueous solution, which is placed in molecular weight retention, after ultrasonication
Bag filter in, be placed in distilled water 12~50h of dialysis, after the Blackfungus polyhexose aqueous solution in bag filter is subjected to vacuum
Freeze-drying, obtains low molecular weight Blackfungus polyhexose.
The black wood for having the beneficial effect that the present invention and hot water being extracted using ultrasonic wave of the present invention compared with the existing technology
The method that fungus polysaccharides carry out degradation treatment, significantly reduces the molecular weight of Blackfungus polyhexose, while viscosity also greatly drops
Low, solubility is preferable in water, and obtained low molecular weight Blackfungus polyhexose has stronger antioxidant activity.It is prepared
Low molecular weight Blackfungus polyhexose can be used as better anti-oxidant, anti-aging, anti-myocardial anoxia and anti-rad, can be used as improvement
The functional food or drug of body physiological function.Present invention process is simple, is suitable for promotion and application.
Specific embodiment
Below with reference to embodiment, further description of the technical solution of the present invention, and however, it is not limited to this, all right
Technical solution of the present invention is modified or equivalent replacement, without departing from the spirit of the technical scheme of the invention range, should all cover
Among protection scope of the present invention.
Specific embodiment 1: present embodiment record is a kind of utilization ultrasonic technology preparation low molecular weight black fungus
The method of polysaccharide, the method comprises the following steps:
Step 1: preparing Blackfungus polyhexose aqueous solution: weighing the Blackfungus polyhexose of certain mass, distilled water is added, by it
It is configured to the Blackfungus polyhexose aqueous solution that mass fraction is 1%~20%；
Step 2: prepared Blackfungus polyhexose aqueous solution is placed in water-bath, bath temperature is 0~100 DEG C；
Step 3: ultrasonic probe is inserted into Blackfungus polyhexose aqueous solution, insertion depth is 1~10cm；
Step 4: the power of adjustment ultrasonic wave is 200~1000W, the processing time is 1~12h；
Step 5: it is 500~3000Da that Blackfungus polyhexose aqueous solution, which is placed in molecular weight retention, after ultrasonication
Bag filter in, be placed in distilled water 12~50h of dialysis, after the Blackfungus polyhexose aqueous solution in bag filter is subjected to vacuum
Freeze-drying, obtaining inherent viscosity is 3~8dL/g, and it is dry powder-shaped that viscosity average molecular weigh, which is the Blackfungus polyhexose of 3400~5000Da,.Phase
Than being respectively 80~98dL/g and 400000~410000Da in the polysaccharide inherent viscosity and viscosity average molecular weigh that are not ultrasonically treated,
Inherent viscosity and molecular weight significantly reduce, while its antioxidant activity improves 1~3 times or more.
Specific embodiment 2: a kind of described in specific embodiment one prepare the black wood of low molecular weight using ultrasonic technology
The method of fungus polysaccharides, in step 1, the mass fraction of the Blackfungus polyhexose aqueous solution is 10%.
Specific embodiment 3: a kind of described in specific embodiment one prepare the black wood of low molecular weight using ultrasonic technology
The method of fungus polysaccharides, in step 2, the bath temperature is 10 DEG C.
Specific embodiment 4: a kind of described in specific embodiment one prepare the black wood of low molecular weight using ultrasonic technology
The method of fungus polysaccharides, in step 3, the insertion depth is 5cm.
Specific embodiment 5: a kind of described in specific embodiment one prepare the black wood of low molecular weight using ultrasonic technology
The method of fungus polysaccharides, in step 4, the power is 500W, and the processing time is 10h.
Specific embodiment 6: a kind of described in specific embodiment one prepare the black wood of low molecular weight using ultrasonic technology
The method of fungus polysaccharides, in step 5, the molecule interception of the bag filter is 3000Da, and the dialysis time is 48h.
Specific embodiment 7: a kind of described in specific embodiment one prepare the black wood of low molecular weight using ultrasonic technology
The method of fungus polysaccharides, in step 5, the condition of the vacuum freeze-drying are as follows: 0.05~0.4mbar of vacuum degree, freeze temperature are -60
~-30 DEG C, 6~12h of freeze-drying time.
Embodiment 1:
A method of low molecular weight Blackfungus polyhexose, the method are prepared using ultrasonic technology specifically:
Step 1: it prepares Blackfungus polyhexose aqueous solution: weighing the Blackfungus polyhexose of certain mass, distilled water is added, by it
It is configured to the Blackfungus polyhexose aqueous solution that mass fraction is 10%；
Step 2: prepared Blackfungus polyhexose aqueous solution is placed in water-bath, and bath temperature is controlled at 10 DEG C；
Step 3: ultrasonic probe is inserted into Blackfungus polyhexose aqueous solution, and insertion depth is controlled in 5cm；
Step 4: the power for adjusting ultrasonic wave is 500W, and the processing time is 10h；
Step 5: after ultrasonication, the Blackfungus polyhexose aqueous solution after ultrasonication is placed in molecular weight and is cut
Be left in the bag filter of 3000Da, be placed in distilled water the 48h that dialyses, after by the Blackfungus polyhexose aqueous solution in bag filter into
Row vacuum freeze-drying (vacuum degree 0.05mbar, freeze temperature are -30 DEG C, freeze-drying time 6h), it is lower to obtain viscosity, antioxidant activity
Stronger low molecular weight Blackfungus polyhexose is dry powder-shaped.
It is 3467Da, inherent viscosity 3.27 that Blackfungus polyhexose viscosity average molecular weigh is obtained in the present embodiment.It obtains simultaneously
There is no significant changes, the low molecular weight Blackfungus polyhexoses to have stronger antioxidant activity, show for Blackfungus polyhexose structure
It can be used as better anti-oxidant, anti-aging, anti-myocardial anoxia and anti-rad, can be used as the function for improving body physiological function
It can property food or drug.It is lower additionally, due to the low-molecular-weight polysaccharide viscosity, therefore can have more in food or medicine field
High application value.
Embodiment 2:
A method of low molecular weight Blackfungus polyhexose, the method are prepared using ultrasonic technology specifically:
Step 1: it prepares the aqueous solution of Blackfungus polyhexose: weighing the Blackfungus polyhexose of certain mass, distilled water is added, it will
It is configured to the Blackfungus polyhexose aqueous solution that mass fraction is 5%；
Step 2: prepared Blackfungus polyhexose aqueous solution is placed in water-bath, and bath temperature is controlled at 10 DEG C；
Step 3: ultrasonic probe is inserted into Blackfungus polyhexose aqueous solution, and insertion depth is controlled in 5cm；
Step 4: the power for adjusting ultrasonic wave is 500W, and the processing time is 10h；
Step 5: after ultrasonication, the Blackfungus polyhexose aqueous solution after ultrasonication is placed in molecular weight and is cut
Be left in the bag filter of 3000Da, be placed in distilled water and dialyse, dialysis time 48h, after will be black in bag filter
Auricularia polysaccharide aqueous solution carries out vacuum freeze-drying (vacuum degree 0.1mbar, freeze temperature are -60 DEG C, freeze-drying time 12h), is glued
Lower, the stronger low molecular weight Blackfungus polyhexose of antioxidant activity is spent, is dry powder-shaped.
Claims (7)
1. a kind of method using ultrasonic technology preparation low molecular weight Blackfungus polyhexose, it is characterised in that: the method step
It is as follows:
Step 1: preparing Blackfungus polyhexose aqueous solution: weighing the Blackfungus polyhexose of certain mass, distilled water is added, is prepared
The Blackfungus polyhexose aqueous solution for being 1%~20% at mass fraction；
Step 2: prepared Blackfungus polyhexose aqueous solution is placed in water-bath, bath temperature is 0~100 DEG C；
Step 3: ultrasonic probe is inserted into Blackfungus polyhexose aqueous solution, insertion depth is 1~10cm；
Step 4: the power of adjustment ultrasonic wave is 200~1000W, the processing time is 1~12h；
Step 5: it is the saturating of 500~3000Da that Blackfungus polyhexose aqueous solution, which is placed in molecular weight retention, after ultrasonication
Analyse in bag, be placed in distilled water 12~50h of dialysis, after the Blackfungus polyhexose aqueous solution in bag filter is subjected to vacuum jelly
It is dry, obtain low molecular weight Blackfungus polyhexose.
2. a kind of method using ultrasonic technology preparation low molecular weight Blackfungus polyhexose according to claim 1, special
Sign is: in step 1, the mass fraction of the Blackfungus polyhexose aqueous solution is 10%.
3. a kind of method using ultrasonic technology preparation low molecular weight Blackfungus polyhexose according to claim 1, special
Sign is: in step 2, the bath temperature is 10 DEG C.
4. a kind of method using ultrasonic technology preparation low molecular weight Blackfungus polyhexose according to claim 1, special
Sign is: in step 3, the insertion depth is 5cm.
5. a kind of method using ultrasonic technology preparation low molecular weight Blackfungus polyhexose according to claim 1, special
Sign is: in step 4, the power is 500W, and the processing time is 10h.
6. a kind of method using ultrasonic technology preparation low molecular weight Blackfungus polyhexose according to claim 1, special
Sign is: in step 5, the molecule interception of the bag filter is 3000Da, and the dialysis time is 48h.
7. a kind of method using ultrasonic technology preparation low molecular weight Blackfungus polyhexose according to claim 1, special
Sign is: in step 5, the condition of the vacuum freeze-drying are as follows: 0.05~0.4mbar of vacuum degree, freeze temperature are -60~-30
DEG C, 6~12h of freeze-drying time.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107903330A (en) * | 2017-11-22 | 2018-04-13 | 广东丸美生物技术股份有限公司 | Degradation of Polysaccharides and low-molecular-weight polysaccharide |
| CN108265092A (en) * | 2017-12-26 | 2018-07-10 | 天津大学 | A kind of mushroom oligosaccharides and preparation method with excellent antioxidant activity |
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- 2019-06-26 CN CN201910560943.2A patent/CN110330573A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107903330A (en) * | 2017-11-22 | 2018-04-13 | 广东丸美生物技术股份有限公司 | Degradation of Polysaccharides and low-molecular-weight polysaccharide |
| CN108265092A (en) * | 2017-12-26 | 2018-07-10 | 天津大学 | A kind of mushroom oligosaccharides and preparation method with excellent antioxidant activity |
Non-Patent Citations (1)
| Title |
|---|
| JUNQIANG QIU等: "Ultrasonic degradation of Polysaccharides from Auricularia auricula and the antioxidant activity of their degradation products", 《LWT - FOOD SCIENCE AND TECHNOLOGY》 * |
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