CN110327030A - Simulate experimental model and its application of coronary artery bridge vascular flow state - Google Patents
Simulate experimental model and its application of coronary artery bridge vascular flow state Download PDFInfo
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Abstract
The present invention provide it is a kind of simulate coronary artery bridge vascular flow situation experimental model and its application.Wherein, the rabbit experimental model of abdominal aorta displacement is close with the coronary artery bridge vascular flow relevant parameter of people, and explore blood flow velocity, the relationship of the size of blood flow and the index of oscillation and variation tendency and vascular patency of experiment blood vessel, the blood flow state of people's coronary artery bridge blood vessel is simulated by the rabbit experimental model that abdominal aorta is replaced, it can promote the basic research of CABG, be conducive to improve the treatment effect of CABG, the development for promoting bridge blood vessel, especially small-caliber artificial blood vessel, gives treatment to the life of patients with coronary heart disease.
Description
Technical field
The invention belongs to field of pharmaceutical biology, in particular to a kind of experimental model for simulating coronary artery bridge vascular flow state and
It is applied.
Background technique
Coronary heart disease is to lead to the primary cause of disease of human death.Coronary bypass surgery (Coronary Artery Bypass
Grafting CABG) it is the surgical method for treating coronary heart disease, long-term effect is best.The blood flow of bridge blood vessel determines coronary disease sufferer
The improvement situation of person's myocardial blood supply and oxygen supply.Bridge blood vessel often selects left side internal mammary artery or great saphenous vein at present, wound when acquisition
Greatly, surgical effect is influenced by the quality of bridge blood vessel, and the patient of second operation may face the available predicament of no blood vessel, and big hidden
10 years plugging rates of vascular grafts are still higher, need to further improve its prognosis.Small-caliber artificial blood vessel patency rate is low, Wu Faying
For CABG.One of the difficult point for accelerating small-caliber artificial blood vessel exploitation is exactly without suitably simulating CABG coronary artery bridge vascular flow
The experimental model of situation.Each region medical level difference in China is big, and CABG operation can be simulated by establishing, simply, economical,
Convenient experimental model is observed, may advantageously facilitate the universal of CABG.The blood flow feelings of current animal model and CABG rear axle blood vessel
Condition difference is obvious, the experimental result obtained using this class model, may can not repeat in the mankind, causes the huge of resource and time
Waste.Therefore, it is necessary to the experimental model that can sufficiently simulate CABG coronary artery bridge vascular flow situation be established, to promote coronary disease
The basic research of disease accelerates the development of CABG artificial blood vessel, improves the treatment effect of CABG, give treatment to the life of patient.
Summary of the invention
Based on this, the purpose of the present invention is to provide a kind of experimental model for simulating coronary artery bridge vascular flow situation and its answer
With.
To achieve the above object, the invention provides the following technical scheme:
A kind of application of the rabbit experimental model of abdominal aorta displacement, which is characterized in that the blood flow of simulation people's coronary artery bridge blood vessel
State, to obtain the blood flow relevant parameter of simulated person's coronary artery bridge blood vessel.
The present invention also provides a kind of method of blood circumstance for simulating people's coronary artery bridge blood vessel, specific technical solution is as follows:
A method of obtaining the blood flow relevant parameter of simulated person's coronary artery bridge blood vessel, which is characterized in that including following step
It is rapid:
(1) the rabbit experimental model of abdominal aorta displacement is obtained；
(2) the blood flow relevant parameter that blood vessel is tested in the rabbit experimental model is measured；
(3) size of blood flow relevant parameter is analyzed and the relationship of change with time trend and experiment vascular patency；
(4) the blood flow relevant parameter and bridge vascular patency of simulated person's coronary artery bridge blood vessel are obtained.
Based on the above-mentioned technical proposal, the invention has the following advantages:
The present invention passes through a large amount of creative works of inventor, the rabbit experimental model of discovery abdominal aorta displacement and the coronary artery of people
Bridge vascular flow relevant parameter is close, and explores blood flow velocity, the size and change of blood flow and the index of oscillation of experiment blood vessel
The relationship of change trend and vascular patency simulates the blood flow shape of people's coronary artery bridge blood vessel by the rabbit experimental model that abdominal aorta is replaced
Condition can promote the basic research of CABG, is conducive to the treatment effect for improving CABG, promotes bridge blood vessel, especially small-bore artificial
The life of patients with coronary heart disease is given treatment in the development of blood vessel.
Detailed description of the invention
Fig. 1-4 is the electron microscope of growth and adherency situation of the endothelial cell on SF-ePTFE group blood vessel；
Fig. 5-6 is that the Electronic Speculum of the artificial blood vessel taken out by common ePTFE blood vessel group observes result.
Specific embodiment
To facilitate the understanding of the present invention, it below with reference to embodiment to invention is more fully described, is given below
Presently preferred embodiments of the present invention.But the invention can be realized in many different forms, however it is not limited to described herein
Embodiment.Purpose of providing these embodiments is makes the disclosure of the present invention more thorough and comprehensive.It should be understood that
In the following examples, the experimental methods for specific conditions are not specified, usually according to normal condition, for example (,) Sambrook et al., molecule gram
It is grand: condition described in laboratory manual (New York:Cold Spring Harbor Laboratory Press, 1989),
Or according to the normal condition proposed by manufacturer.Used various common agents, are commercial product in embodiment.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Used term is intended merely to describe specific reality in the description of the invention
Apply the purpose of example, it is not intended that in the limitation present invention.Term as used herein "and/or" includes one or more relevant institutes
Any and all combinations of list of items.
A kind of application of the rabbit experimental model of abdominal aorta displacement, simulates the blood flow state of people's coronary artery bridge blood vessel, to obtain
The blood flow relevant parameter of simulated person's coronary artery bridge blood vessel.A kind of side for the blood flow relevant parameter obtaining simulated person's coronary artery bridge blood vessel
Method, comprising the following steps: (1) obtain the rabbit experimental model of abdominal aorta displacement；(2) it measures in the rabbit experimental model and tests blood
The blood flow relevant parameter of pipe；(3) size of blood flow relevant parameter is analyzed and change with time trend and experiment vascular patency
Relationship；(4) the blood flow relevant parameter and bridge vascular patency of simulated person's coronary artery bridge blood vessel are obtained.Wherein, the abdominal aorta
Displaced rabbit experimental model are as follows: abdominal aorta part is by the rabbit of experiment vascular replacement, the abdomen master of the experiment blood vessel and the rabbit
Artery end to end anastomosis.
Specifically, application of the present invention are as follows: test the blood flow state of blood vessel in the rabbit experimental model by measuring
Relevant parameter, for simulating the relevant parameter of blood flow state of people's coronary artery bridge blood vessel.Optionally, the blood flow state is relevant
Parameter includes: blood flow velocity, mean blood flow, the index of oscillation, leucocyte numerical value, red blood cell numerical value, blood platelet numerical value, PT (solidifying
Blood zymogen time), APTT (activated partial thromboplastin time), TT (thrombin time).
Further, the present invention also pass through explore tested in rabbit experimental model the blood flow velocity of blood vessel, mean blood flow and
The size of the index of oscillation and the relationship of vascular patency, to simulate blood flow velocity, mean blood flow and the wave of people's coronary artery bridge blood vessel
The size of dynamic index and the relationship of vascular patency.
In some embodiments of the invention, in the rabbit experimental model of above-mentioned abdominal aorta displacement, the abdomen master of rabbit is replaced
The experiment blood vessel of artery includes but is not limited to the self jugular vein, ePTFE blood vessel or sulfonated fibroin albumen membrane modifying of rabbit
EPTFE blood vessel (SF-ePTFE blood vessel).In some other embodiment, the abdomen of other artificial blood vessel permutation graphs can also be selected
Aorta, to adapt to the demand of simulation coronary artery bridge blood vessel.
Application of the rabbit experimental model of abdominal aorta displacement of the present invention in the screening of coronary artery bridge blood vessel.The present invention
In, the rabbit experimental model of abdominal aorta displacement can test the blood flow state correlation of blood vessel by measuring in the rabbit experimental model
Parameter, for simulating the relevant parameter of blood flow state of people's coronary artery bridge blood vessel.The wherein rabbit experiment of the abdominal aorta displacement
Model can be abdominal aorta part by it is any experiment vascular replacement rabbit, it is described experiment blood vessel can be need screen can
With the experiment blood vessel for coronary artery bridge blood vessel.By the rabbit experimental model of different experiments vascular replacement, different experiments blood vessel is simulated
It is used for the relevant parameter of blood flow state when CABG, as coronary artery bridge blood vessel to obtain the coronary artery bridge blood vessel being more suitable for.
A kind of method of the blood flow relevant parameter of acquisition simulated person coronary artery bridge blood vessel of the invention, comprising the following steps:
(1) the rabbit experimental model of abdominal aorta displacement is obtained；(2) the blood flow correlation ginseng that blood vessel is tested in the rabbit experimental model is measured
Number；(3) size of blood flow relevant parameter is analyzed and the relationship of change with time trend and experiment vascular patency；(4) mould is obtained
The blood flow relevant parameter and bridge vascular patency of quasi- people's coronary artery bridge blood vessel.
Optionally, the rabbit experimental model of the abdominal aorta displacement includes but is not limited to ePTFE vascular replacement abdominal aorta
Rabbit experimental model or SF-ePTFE vascular replacement abdominal aorta rabbit experimental model.
Specifically, the relevant parameter of the blood flow state includes: blood flow velocity, mean blood flow, the index of oscillation, leucocyte
Numerical value, red blood cell numerical value, blood platelet numerical value, prothrombin time, activated partial thromboplastin time, thrombin time, fiber
Proteinogen content.
Zoopery of the present invention is ratified by experimental animal Ethics Committee, Nanfang Medical Univ.
1 rabbit abdominal aorta replacement operation of embodiment establishes simulation coronary artery bridge vascular flow situation experimental model
Anesthesia: using 1% amobarbital intravenous anesthesia+lidocaine wound local infiltration anesthesia method.
Jugular vein group: use Adult New Zealand White Rabbit, 40, by isolated after rabbit anesthesia right side arteria carotis communis with
Jugular vein, vena jugularis externa.Then arteria carotis communis intubation test rabbit blood pressure, jugular vein separation length 5cm block both ends, cut
Jugular vein 3cm is removed, the endovascular blood of normal saline flushing is spare, by two end-to-end anastomosis of jugular vein to vena jugularis externa
On.Rabbit abdominal aorta replacement operation selects lower 1/3, length about 5cm, exposure in notch abdomen simultaneously to separate abdominal aorta, and ligation is set
Huan Duan abdominal aorta branch records branch's number, blocks abdominal aorta after test tube of hepari, and break abdominal aorta 2cm later, is kissed using end end
The method of conjunction, jugular vein and abdominal aorta are coincide.
EPTFE group and SF-ePTFE group: method of the method with jugular vein displacement abdominal aorta.EPTFE group and SF-
EPTFE group is tested each 40 with rabbit.
Wherein, in above-mentioned SF-ePTFE group, the method for modifying of SF-ePTFE are as follows: first use argon Low Temperature Plasma Treating
EPTFE blood vessel, parameter are as follows: discharge voltage pressure 70Pa, discharge power 60W, discharge time 20min.1.5mg/ml are used later
Fibroin liquid applies inner wall, then handles fibroin protein film ptfe composite blood vessel, parameter with sulfur anhydride plasma are as follows:
Flow: 20SCCM, pressure: 20Pa, discharge power: 60W handles the time: 20min.Postoperative to give penicillin prevention infection, 6 is small
When after give aspirin anti-poly- treatment, dosage 3mg/kg, while giving ranitidine and prevent gastric ulcer.First post-operative day is opened
Beginning gives raising in the conventional independent cage of rabbit grain.
CABG patient with operation: choosing coronary heart disease row CABG patient 20, and operation method recycles lower CABG using routine in vitro,
Left side internal mammary artery end-to-side anastomosis to descending anterior branch blood vessel, the bridge blood vessel of remaining coronary artery uses great saphenous vein.
(1) preoperative comparison: preoperative 2 days measurement control groups, by taking SF-ePTFE group as an example, by rabbit abdominal aorta replacement model
Blood pressure, leucocyte numerical value, red blood cell numerical value, blood platelet numerical value, PT, APTT, TT, FIB, the relevant parameter pair with CABG patient
Than observation is preoperative in terms of blood constituent, coagulation function, whether has difference with CABG patient.The results are shown in Table 1.
1 rabbit abdominal aorta replacement model parameter of table and the preoperative parameter comparison of people CABG
| Parameter | People CABG | Rabbit abdominal aorta replacement model parameter |
| Systolic pressure | 90-140mmHg | 100±8mmHg |
| Diastolic pressure | 60-90mmHg | 80±11mmHg |
| Leucocyte | 4×109-10×109/L | 7.5-13.5×109/L |
| Red blood cell | 4×1012-5.5×1012/L | 4-7.2×1012/L |
| Blood platelet | 100×109-300×109/L | 280±20×109/L |
| Blood vessel diameter | 1-2mm | 2.30±0.42mm |
| Blood flow | Greater than 35ml/min (great saphenous vein bridge) | 32-51ml/min (rabbit abdominal aorta) |
| APTT | 28-45s | 55±5s |
| TT | 14-21s | 10±0.5s |
| PT | 11-15s | 14±1s |
It, can be in blood pressure side it can be found that the systolic pressure of rabbit and diastolic pressure and the blood pressure of the mankind are not much different in upper table
The case where mankind's blood pressure, is preferably simulated in face, this has preferable advantage, big animal model, such as baboon in small animal model
Primate is more nearly with people, but economic cost is higher, and when being able to use toy progress zoopery, ethics is wanted
It asks and uses toy as far as possible.Baboon can in terms of blood pressure or blood coagulation close to the mankind, but in terms of blood flow and blood vessel diameter, with
People has obvious difference again.
In terms of haemocyte, leucocyte, red blood cell and blood platelet have the place being overlapped with the mankind, can simulate mankind's blood
The case where cell.
In terms of blood coagulation, APTT, TT, PT and the mankind of rabbit are not much different, and can preferably simulate the change of mankind's blood coagulation.
As it can be seen that abdominal aorta 2.30 ± 0.42mm of diameter of rabbit, diameter are suitable with the diameter of people's coronary artery.Rabbit abdominal aorta
Blood flow about 72 ± 15ml/min is suitable with the blood flow of great saphenous vein bridge after CABG.So using the abdominal aorta of rabbit come structure
The experimental model for building CABG bridge blood vessel, from blood constituent, coagulation function, blood vessel diameter and blood flow etc. consider, reasonable
By upper feasibility.
(2) it is compared when operation is completed: by taking SF-ePTFE group as an example, when operation is finished, rabbit abdominal aorta replacement model two
At side-to-side anastomosis mouth and experiment blood vessel among blood flow velocity, mean blood flow, the index of oscillation, and with CABG patient's bridge vascular anastomosis
Whether blood flow velocity, mean blood flow, the index of oscillation at mouthful compare, variant between observation each group index.As a result such as 2 institute of table
Show.
2 rabbit abdominal aorta replacement model of table and the postoperative parameter comparison of people CABG
| Blood flow velocity | Mean blood flow | The index of oscillation | |
| CABG (great saphenous vein bridge) | 40±10cm/s | 90±21ml/min | 1.5±0.3 |
| Rabbit abdominal aorta replacement model | 42±5cm/s | 72±15ml/min | 2.1±0.4 |
| Statistical difference | P>0.05 | P>0.05 | P<0.05 |
It is found through measurement, the blood flow velocity of rabbit abdominal aorta, with the blood flow velocity of great saphenous vein bridge blood vessel to close, comparison two
The no significant difference of group.
It, can the good blood for simulating coronary artery bypass grafting rear axle blood vessel with rabbit abdominal aorta replacement operation in terms of blood flow velocity
Flow velocity degree changes.
In terms of blood flow, the blood flow of rabbit abdominal aorta and the blood flow of great saphenous vein bridge are not much different, statistics pair
Than having no statistical difference.
On the index of oscillation, the index of oscillation of rabbit abdominal aorta is higher than the previous anastomotic of people's coronary artery bypass grafting great saphenous vein bridge,
It is variant related compared with people's coronary artery with the elasticity of rabbit abdominal aorta, in addition, bridge blood vessel side mouth is longer when coronary artery bypass grafting, about
8mm, notch uses end to end anastomosis plus side face prolonged incison when this model construction, this may be to lead to the discrepant original of the index of oscillation
Cause.But the two is small compared to discrepant quantity, is presently the most the small animal model close with coronary bypass surgery.
Specific assay method is as follows for above-mentioned parameter:
CABG patient group: the blood flow velocity at each CABG bridge vascular anastomosis, including a left side are measured with heart Vascular Ultrasonography machine
Then side internal mammary artery bridge vascular anastomosis and great saphenous vein bridge vascular anastomosis detect bridge vascular anastomosis with Doppler's flow instrument
Mean blood flow and the index of oscillation at mouthful.
Jugular vein group, ePTFE group, SF-ePTFE group and control group rabbit: abdominal aorta at lower 1/3 in measurement lower abdomen
Blood flow velocity, mean blood flow and the index of oscillation.
Wherein, 5 cardiac cycle of measurement of mean blood flow finds out the average value (Qmean) of blood flow.The index of oscillation is
The difference of maximum blood flow (Qmax) and minimum blood flow (Qmin) in cardiac cycle.
Formula are as follows: (Qmax-Qmin)/Qmean
The measurement of blood flow velocity uses Philip iE33 Vascular Ultrasonography machine, and the test of mean blood flow and the index of oscillation is used
The measurement of TF1100 Doppler's flow detector.
2 blood flow state parameter of embodiment changes over time trend and blood vessel embolism rate
To in the operation of the vascular replacement of SF-ePTFE group rabbit, blood flow velocity using 180cm/s as critical value, mean blood flow with
35 be cut off value, compares the relationship of blood flow velocity and mean blood flow and thrombosis rate respectively.The results are shown in Table 3.
The relationship of table 3 blood flow velocity and mean blood flow and thrombosis rate
According to 3 result of table it can be found that the embolism and blood flow velocity of artificial blood vessel have related, Hemodynamic environment to mean blood flow
Degree increases, and especially obviously increases, is often associated with the luminal stenosis of artificial blood vessel, and displaced rabbit artificial blood vessel can be easy to block,
Especially blood flow velocity is more than 180cm/s, and comparison blood flow velocity is more than 180cm/s group rabbit and the experimental rabbit lower than the numerical value, hair
Both existing embolism probabilities have notable difference, statistically significant.
In terms of mean blood flow, the embolism probability of the two also has notable difference, comparison discovery, and mean blood flow is more than
35ml/min and there is notable difference lower than the experiment thrombosis rate of rabbit of 35ml/min group.This and the great saphenous vein clinically found
The blood flow of bridge blood vessel is similar with the relationship picture of vascular patency, and the blood flow of great saphenous vein bridge is clinically required to be more than at present
45ml/min, otherwise bridge blood vessel is easy to happen blocking.The conclusion of this experiment may bring positive influences for CABG operations research, such as
The blood flow of CABG post-surgical vascular is relatively low or blood flow velocity is excessively high, may indicate and be easy to block after surgery in bridge blood vessel,
It needs further to handle.
3 endothelial cell of the embodiment speed of growth and connection type in different blood vessel
By the observation to jugular vein group, ePTFE group, SF-ePTFE group blood vessel in rabbit abdominal aorta, it is thin to understand endothelium
The speed of growth and connection type of the born of the same parents in different blood vessel.
Observe object: selected part artificial blood vessel unobstructed test rabbit, Yu Shuhou 6 months taking-up ePTFE groups, SF-
The displacement blood vessel of ePTFE group, Fig. 1-4 is SF-ePTFE group blood vessel, and Fig. 5-6 is the blood vessel of ePTFE group.It is scanned Electronic Speculum sight
It examines.
Electronic Speculum observation: using the growing state of scanning electron microscopic observation blood vessel endothelial cell, endothelial cell growth and company
The mode connect, including longitudinal and lateral connection.EPTFE group, SF-ePTFE group blood vessel and endothelium are observed under transmission electron microscope
Adherency and connection type between cell, the microstructure of connection.As a result as shown in figures 1 to 6.As it can be seen that Fig. 1 to Fig. 4 is that endothelium is thin
Growth and adherency situation of the born of the same parents on SF-ePTFE group blood vessel, it can be seen that endothelial cell is longitudinal on SF-ePTFE group blood vessel
Connection growth, while lateral connection.Fig. 5 to Fig. 6 discovery: the upper visible platelet aggregation of the artificial blood vessel of common ePTFE blood vessel group,
Pseudopodium growth, the thrombus that red blood cell is formed show that common PTFE blood vessel is easier embolism in thin vessels.
According to the result of this experiment it is found that rabbit abdominal aorta displacement rabbit experimental model can be applied to CABG instrument or
The research of drug improves CABG operation skill to obtain better surgical treatment, promotes the basic research of coronary heart disease.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to the above reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of application of the rabbit experimental model of abdominal aorta displacement, which is characterized in that the blood flow shape of simulation people's coronary artery bridge blood vessel
State, to obtain the blood flow relevant parameter of simulated person's coronary artery bridge blood vessel.
2. application according to claim 1, which is characterized in that the rabbit experimental model of the abdominal aorta displacement are as follows: abdomen master
Arterial portions are by the rabbit of experiment vascular replacement, the abdominal aorta end to end anastomosis of the experiment blood vessel and the rabbit.
3. application according to claim 2 characterized by comprising test blood by measuring in the rabbit experimental model
The blood flow relevant parameter of pipe, for simulating the blood flow relevant parameter of people's coronary artery bridge blood vessel.
4. application according to claim 3, which is characterized in that the blood flow relevant parameter includes: blood flow velocity, average blood
Flow, the index of oscillation, leucocyte numerical value, red blood cell numerical value, blood platelet numerical value, prothrombin time, activated partial thromboplastin
Time, thrombin time, fibrinogen content.
5. application according to claim 3 characterized by comprising test blood by measuring in the rabbit experimental model
Pipe and blood flow velocity, mean blood flow and the index of oscillation at the previous anastomotic of abdominal aorta, for simulating people's coronary artery bridge blood vessel kiss
It heals up and locates blood flow velocity, mean blood flow and the index of oscillation；And/or
Endovascular blood flow velocity, mean blood flow and the index of oscillation are tested by measuring in the rabbit experimental model, is used for mould
The endovascular blood flow velocity of anthropomorphic coronary artery bridge, mean blood flow and the index of oscillation；And/or
By the leucocyte numerical value, red blood cell numerical value, blood platelet numerical value prothrombin time, activation that measure the rabbit experimental model
Partial thromboplastin time, thrombin time, fibrinogen content, for simulating the leucocyte for having the people of coronary artery bridge blood vessel
Numerical value, red blood cell numerical value, blood platelet numerical value, PT, APTT, TT, FIB.
6. application according to claim 2, which is characterized in that by rabbit experimental model test blood vessel blood flow velocity,
The relationship of the size and vascular patency of mean blood flow and the index of oscillation simulates the blood flow velocity of people's coronary artery bridge blood vessel, is averaged
The relationship of the size and vascular patency of blood flow and the index of oscillation.
7. according to the described in any item applications of claim 2~6, which is characterized in that the experiment blood vessel is selected from: quiet in the neck of rabbit
Arteries and veins, ePTFE blood vessel or sulfonated fibroin albumen membrane modifying ePTFE blood vessel.
8. a kind of method for the blood flow relevant parameter for obtaining simulated person's coronary artery bridge blood vessel, which comprises the following steps:
(1) the rabbit experimental model of abdominal aorta displacement is obtained；
(2) the blood flow relevant parameter that blood vessel is tested in the rabbit experimental model is measured；
(3) size of blood flow relevant parameter is analyzed and the relationship of change with time trend and experiment vascular patency；
(4) the blood flow relevant parameter and bridge vascular patency of simulated person's coronary artery bridge blood vessel are obtained.
9. the method for the blood flow relevant parameter according to claim 8 for obtaining simulated person's coronary artery bridge blood vessel, feature exist
In the rabbit experimental model of the abdominal aorta displacement are as follows: abdominal aorta part is by the rabbit of experiment vascular replacement, the experiment blood vessel
With the abdominal aorta end to end anastomosis of the rabbit.
10. the method for the blood flow relevant parameter of acquisition simulated person's coronary artery bridge blood vessel according to claim 8 or claim 9, special
Sign is, the relevant parameter of the blood flow state includes: blood flow velocity, mean blood flow, the index of oscillation, leucocyte numerical value, red
Cell numerical value, blood platelet numerical value, prothrombin time, activated partial thromboplastin time, thrombin time, fibrinogen contain
Amount.
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| CN111803050A (en) * | 2020-07-01 | 2020-10-23 | 唐科伦 | Great saphenous vein availability degree analysis system |
| CN112712586A (en) * | 2020-12-22 | 2021-04-27 | 复旦大学附属中山医院 | Rapid generation method of artery model |
| CN113408152A (en) * | 2021-07-23 | 2021-09-17 | 上海友脉科技有限责任公司 | Coronary artery bypass transplantation simulation system, method, medium and electronic device |
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