CN110314664A - A kind of partial size monodisperse HbA1C ion-exchange chromatography filler synthetic method and its application - Google Patents
A kind of partial size monodisperse HbA1C ion-exchange chromatography filler synthetic method and its application Download PDFInfo
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Abstract
The invention discloses a kind of partial size monodisperse HbA1C ion-exchange chromatography filler synthetic methods, prepare carrier oil phase by colloidal sol and oil phase emulsion, and obtain using polymer seeds swelling method.The present invention provides a kind of partial size monodisperse HbA1C ion-exchange chromatography filler synthetic methods, it innovatively uses using colloidal sol as carrier, carrier oil phase is prepared in conjunction with oil phase emulsion, partial size monodispersity ion-exchange chromatography filler is obtained using polymer seeds swelling method, with outstanding stability, associativity and controllability, the disadvantage for avoiding the ion exchange capacity of the existing ion-exchange chromatography filler prepared by rear modification method uncontrollable substantially reduces chromatograph packing material and acts on the non-specific adsorption of glycosylated hemoglobin；And preparation method is simple, global controllability is strong, helps to realize the production of planningization, mass.
Description
Technical field
The invention belongs to ion-exchange chromatography filler preparation fields, more particularly to a kind of partial size monodisperse HbA1C ion
Exchange chromatography filler synthetic method and its application.
Background technique
Diabetes are a kind of chronic metabolic derangements diseases caused by being increased by blood glucose level, can lead to multiple complications,
Such as kidney trouble, heart disease, neurotrosis.Glycosylated hemoglobin be hemoglobin beta chain N-terminal valine free amine group with not
A kind of glycosylated hemoglobin to be formed is glycosylated with carbohydrate, synthesis process is slow, irreversible, and not by daily glucose
Horizontal influence of fluctuations, therefore be one of standard of diabetes diagnosis.
Wherein, hemoglobin (Hb) and the unbonded part of sugar are HbA0, bound fraction HbA1, according to the carbon water combined
The difference of compound can be divided into HbA1a1, HbA1a2, HbA1b and HbA1c, and wherein HbA1c accounts for about the 80% of HbA1
(T.M.Thevarajah, N.Nani, Y. Y. Chew. Performance evaluation of the arkray
Adams HA-8160 HbA1C analyser [J] Malays J Pathol, 2008,30 (2): 81-86), and its content
It is positively correlated, and can keep about 120 days in apparent with blood sugar concentration content, can directly react the blood in diabetic 8-12 weeks
Sugar control situation.There are many method of measurement saccharification hemoglobin content at present, mainly include electrophoresis, ion-exchange chromatography, height
It imitates ion exchange in solution chromatography (HPLC), boric acid affinity chromatography, method etc. is used in conjunction in immunoassay and liquid matter, wherein HPLC quilt
" goldstandard " of international clinical chemistry and experimental medicine federation as detection glycosylated hemoglobin.
Seed swelling method is that small particle is made first with emulsion polymerization cooperation emulsifier-free emulsion polymerization or dispersion polymerization processes
Monodispersed polymer seeds are swollen with polymerisable monomer, initiator, crosslinking agent etc., are that seed microballoon becomes larger, then again
It is polymerize, the method so as to which the monodispersed polymer microballoon of partial size is made.It is that can stablize at present, in high volume synthesize
The main method of monodisperse polymer micro-sphere.And traditional suspension polymerization, precipitation polymerization method, the preparations such as dispersion copolymerization method it is micro-
Spherolite diameter is larger and distribution is wide, it is difficult to and it is directly used as chromatography chromatographic field, is needed by being strictly screening and precipitation process, and
It is differed between batch larger, it is difficult to carry out large-scale production, therefore quality control requirement is extremely stringent.
Therefore, developing a kind of ion-exchange chromatography filler for detecting glycosylated hemoglobin in view of the above problems is this
Field technical staff institute urgent need to solve the problem.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of partial size monodisperse HbA1C ion-exchange chromatography filler conjunction
At method.In order to achieve the above object, the invention provides the following technical scheme: a kind of partial size monodisperse HbA1C ion exchange
Chromatograph packing material synthetic method prepares carrier oil phase by colloidal sol and oil phase emulsion,
And it is obtained using polymer seeds swelling method.Further, quality shared by the constituent of carrier oil phase and each ingredient
Number is respectively as follows: sol vehicle: 35~55 parts, oil phase emulsion: 17~25 parts, filling component: 7~11 parts and auxiliary components: 4
~9 parts.Further, the specific steps are as follows: (1) raw material preparation of carrier oil phase: weigh oil phase monomer, crosslinking agent and emulsification
Agent obtains oil phase emulsion after emulsifying 5~20min after mixing；Sol vehicle is taken, addition is oily thereto after heating fusing
Phase emulsion stirs evenly；Filling component is weighed simultaneously and auxiliary components are spare；(2) carrier oil is mutually prepared: in step (1)
Filling component is added in sol vehicle added with oil phase emulsion and auxiliary components are polymerize, it is mutually spare to obtain carrier oil；
(3) prepared by water phase；Stabilizer is taken, ionized water is added, rising temperature for dissolving is cold after stabilizer is dissolved completely in deionized water
But to room temperature, water phase is obtained；(4) oil is mutually swollen: the carrier oil obtained in step (2) is added to seed polymer microballoon
In, it stirs evenly, keeps system temperature 0.5~2h at 14~85 DEG C, obtain oil mutually swelling object；(5) cause solvent swell: taking
Initiator, emulsifier and water are mixed, and are added in the oil phase swelling object that step (4) obtain after emulsifying 5~20min；(6) gather
It closes: step (3) being obtained into water phase and are added in oil phase swelling object, are warming up to 75~82 DEG C after mixing evenly, and keep the temperature 10~40h；
After polymerization, it is cooled to room temperature acquisition polymer microballoon；(7) it cleans: the polymer microballoon that step (6) obtain is drained, and
It is cleaned with solvent, obtains partial size monodisperse HbA1C ion-exchange chromatography filler.Further, the specific steps of step (2) are such as
Under: the sol vehicle added with oil phase emulsion is transferred in pre-transform teactor by (2-a), carries out preliminary polymerization, and generation includes
The mixture of oil phase emulsion, sol vehicle and carrier colloidal sol；(2-b) separates the mixture in step (2-a), generates
Include the first logistics of 65~92wt% of mixture in step (2-a) and the second logistics comprising surplus；(2-c) is to second
The first logistics is added in reactor, is further polymerize, and the inversion of phases of the first logistics is caused；(2-d) will be in step (2-c)
The second logistics obtained in the first logistics of inversion of phases and step (2-b) is with 25~33s-1Shear rate in static mixer
Mixing carries out second of inversion of phases, generates gelatinous primary carrier oil phase；The primary carrier oil that (2-e) obtains step (2-d)
Melten gel is mutually carried out, and filling component and auxiliary components are added, stirring forms carrier oil phase fluid, obtains carrier oil after extrusion
Phase.Further, filling component is polymer filler, for appointing in poly- methylpropionate and polystyrene-divinylbenzene
It anticipates one or two kinds of combination.Further, mass fraction shared by the constituent of auxiliary components and each ingredient is respectively as follows:
Curing agent: 13~20 parts, expanding material: 11~20 parts, dispersing agent: 10~18 parts, levelling agent: 10~16 parts, stabilizer: 10~15
Part, hydrogen peroxide: 10~15 parts and phosphoric acid aluminium liquid: 8~12 parts.Further, oil phase monomer includes the first monomer, second comonomer
And Third monomer；First monomer be styrene, methyl styrene, ethyl styrene, 1-chloro-4-methyl-benzene, methyl acrylate,
Propyl acrylate, butyl acrylate, methyl methacrylate, tetramethacrylate, methyl propenoic acid glycidyl
Any one in ester, glycidyl acrylate and butyl methacrylate or any several combination；Second comonomer
For in acrylamide or hydroxyethyl methacrylate, hydroxy-ethyl acrylate and Methacrylamide any one or
Any several combination；Third monomer is 2- acrylamide-2-methyl propane sulfonic；Crosslinking agent be divinylbenzene, two propylene benzene,
Ethyleneglycol dimethyacrylate, triethylene acid glyceride, trihydroxy methyl oxypropyl trimethyl acrylic acid, three acrylic acid of pentaerythrite
Ester, dimethacrylate, triethylene-glycol dimethylacrylate, tetraethylene-glycol dimethacrylate
Ester, 1,3 butylene glycol dimethylacrylate, 1,4- butanediol dimethylacrylate hexamethylene bis Methacrylamide, two
Vinyl benzene methylmethane, trimethacrylate acid glyceride, any one or any several group in methylene acrylamide
It closes；Emulsifier is ionic emulsifying agent.Further, stabilizer is polyvinyl alcohol, polyethylene glycol, polyvinylpyrrolidone, hydroxyl
Methylcellulose, hydroxypropyl cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, beta-cyclodextrin, Beta-methyl cyclodextrin and hydroxyl
Any one in base apatite or any several combination.Further, initiator is benzoyl peroxide, peroxidating two
Any one in isobutyronitrile, azo-bis-iso-dimethyl and azo diisobutyl amidine or any several combination.One
The method of kind measurement saccharification hemoglobin content, passes through efficient liquid phase using partial size monodisperse HbA1C ion-exchange chromatography filler
Ion-exchange chromatography is measured.The present invention provides a kind of partial size monodisperse HbA1C ion-exchange chromatography filler synthesis sides
Method, innovatively uses using colloidal sol as carrier, prepares carrier oil phase in conjunction with oil phase emulsion, obtains grain using polymer seeds swelling method
Diameter monodispersity ion-exchange chromatography filler has outstanding stability, associativity and controllability, avoids existing repairing after
The uncontrollable disadvantage of the ion exchange capacity of the ion-exchange chromatography filler of decorations method preparation, substantially reduces chromatograph packing material to saccharification
The non-specific adsorption of hemoglobin acts on；And preparation method is simple, global controllability is strong, helps to realize planningization, criticizes
The production of quantization.
Detailed description of the invention
Fig. 1 is the SEM figure for the partial size monodisperse HbA1C ion-exchange chromatography filler that the present embodiment 1 synthesizes.
Specific embodiment is described in detail technical solution provided by the invention below with reference to specific embodiment, answers
Understand following specific embodiment parties
Formula is only illustrative of the invention and is not intended to limit the scope of the invention.A kind of embodiment 1: partial size monodisperse HbA1C
Ion-exchange chromatography filler synthetic method prepares carrier oil phase by colloidal sol and oil phase emulsion,
And it is obtained using polymer seeds swelling method；The difference of mass fraction shared by the constituent of carrier oil phase and each ingredient
Are as follows: sol vehicle: 35~55 parts, oil phase emulsion: 17~25 parts, filling component: 7~11 parts and auxiliary components: 4~9 parts；It fills out
Filling component is polymer filler, for poly- methylpropionate；Mass fraction shared by the constituent of auxiliary components and each ingredient point
Not are as follows: curing agent: 13 parts, expanding material: 20 parts, dispersing agent: 10 parts, levelling agent: 16 parts, stabilizer: 10 parts, hydrogen peroxide: 15 parts
And phosphoric acid aluminium liquid: 8 parts.
Specific step is as follows: (1) raw material preparation of carrier oil phase: weighing oil phase monomer, crosslinking agent and emulsifier, is uniformly mixed
Afterwards, oil phase emulsion is obtained after emulsifying 5~20min；Sol vehicle is taken, oil phase emulsion is added thereto after heating fusing, stirs
Uniformly；Filling component is weighed simultaneously and auxiliary components are spare；
Sol vehicle added with oil phase emulsion is transferred in pre-transform teactor by (2-a), carries out preliminary polymerization, and generation includes
The mixture of oil phase emulsion, sol vehicle and carrier colloidal sol；(2-b) separates the mixture in step (2-a), generates
Include the first logistics of 65~92wt% of mixture in step (2-a) and the second logistics comprising surplus；(2-c) is to second
The first logistics is added in reactor, is further polymerize, and the inversion of phases of the first logistics is caused；(2-d) will be in step (2-c)
The second logistics obtained in the first logistics of inversion of phases and step (2-b) is with 25~33s-1Shear rate in static mixer
Mixing carries out second of inversion of phases, generates gelatinous primary carrier oil phase；The primary carrier oil that (2-e) obtains step (2-d)
Melten gel is mutually carried out, and filling component and auxiliary components are added, stirring forms carrier oil phase fluid, obtains carrier oil after extrusion
Phase；
(3) prepared by water phase；Stabilizer is taken, ionized water is added, rising temperature for dissolving is dissolved completely in deionized water to stabilizer
Afterwards, it is cooled to room temperature, obtains water phase；
(4) oil is mutually swollen: the carrier oil obtained in step (2) being added in seed polymer microballoon, is stirred evenly, is kept
System temperature 0.5~2h at 14~85 DEG C obtains oil mutually swelling object；
(5) cause solvent swell: taking initiator, emulsifier and water and mixed, addition step (4) obtains after emulsifying 5~20min
The oil obtained is mutually in swelling object；
(6) it polymerize: step (3) is obtained into water phase and are added in oil phase swelling object, are warming up to 75~82 DEG C after mixing evenly, and keep the temperature
10~40h；After polymerization, it is cooled to room temperature acquisition polymer microballoon；
(7) clean: the polymer microballoon that step (6) obtain being drained, and is cleaned with solvent, obtain partial size monodisperse HbA1C from
Sub- exchange chromatography filler.
Oil phase monomer includes the first monomer, second comonomer and Third monomer in above step (1)；First monomer is benzene second
Alkene, methyl styrene, ethyl styrene, 1-chloro-4-methyl-benzene, methyl acrylate, propyl acrylate, butyl acrylate, methyl-prop
E pioic acid methyl ester, tetramethacrylate, glycidyl methacrylate, glycidyl acrylate and methyl-prop
Combination in olefin(e) acid butyl ester；Second comonomer is acrylamide or hydroxyethyl methacrylate, hydroxy-ethyl acrylate and methyl
Combination in acrylamide；Third monomer is 2- acrylamide-2-methyl propane sulfonic；Crosslinking agent is divinylbenzene, two propylene
Benzene, ethyleneglycol dimethyacrylate, triethylene acid glyceride, trihydroxy methyl oxypropyl trimethyl acrylic acid, pentaerythrite 3 third
Olefin(e) acid ester, dimethacrylate, triethylene-glycol dimethylacrylate, tetraethylene-glycol dimethyl propylene
Olefin(e) acid ester, 1,3 butylene glycol dimethylacrylate, 1,4- butanediol dimethylacrylate hexamethylene bis methacryl
The combination of amine, divinyl phenylmethane, trimethacrylate acid glyceride, methylene acrylamide；Emulsifier is ionic cream
Agent.Stabilizer is polyvinyl alcohol, polyethylene glycol, polyvinylpyrrolidone, hydroxymethyl cellulose, hydroxypropyl cellulose, hydroxyl second
Combination in base cellulose, carboxymethyl cellulose, beta-cyclodextrin, Beta-methyl cyclodextrin and hydroxyapatite.Initiator was
Combination in Benzoyl Oxide, peroxidating bis-isobutyronitrile, azo-bis-iso-dimethyl and azo diisobutyl amidine.
The partial size monodisperse HbA1C ion-exchange chromatography filler that institute's providing method and component synthesize through this embodiment
SEM figure is as shown in Figure 1.
Embodiment 2:
A kind of method for measuring saccharification hemoglobin content is present embodiments provided, by using acquisition partial size list in embodiment 1
Disperse HbA1C ion-exchange chromatography filler, is measured by efficient liquid phase ion-exchange chromatography.
Finally, it should be noted that property technical side the above examples are only used to illustrate the technical scheme of the present invention and are not limiting
Case, those skilled in the art should understand that, modification or equivalent replacement of the technical solution of the present invention are made for those, and
The objective and range for not departing from the technical program, are intended to be within the scope of the claims of the invention.
Claims (10)
1. a kind of partial size monodisperse HbA1C ion-exchange chromatography filler synthetic method, it is characterised in that: pass through colloidal sol and oily mutually cream
Liquid prepares carrier oil phase, and is obtained using polymer seeds swelling method.
2. a kind of partial size monodisperse HbA1C ion-exchange chromatography filler synthetic method according to claim 1, feature exist
In: mass fraction shared by the constituent of the carrier oil phase and each ingredient is respectively as follows: sol vehicle: 35~55 parts, oily phase
Lotion: 17~25 parts, filling component: 7~11 parts and auxiliary components: 4~9 parts.
3. a kind of partial size monodisperse HbA1C ion-exchange chromatography filler synthetic method according to claim 2, feature exist
In: specific step is as follows: (1) raw material preparation of carrier oil phase: weighing oil phase monomer, crosslinking agent and emulsifier, is uniformly mixed
Afterwards, oil phase emulsion is obtained after emulsifying 5~20min；Sol vehicle is taken, oil phase emulsion is added thereto after heating fusing, stirs
Uniformly；Filling component is weighed simultaneously and auxiliary components are spare；(2) carrier oil is mutually prepared: mutually newborn added with oil in step (1)
Filling component is added in the sol vehicle of liquid and auxiliary components are polymerize, it is mutually spare to obtain carrier oil；(3) prepared by water phase；
Stabilizer is taken, ionized water is added, rising temperature for dissolving is cooled to room temperature after stabilizer is dissolved completely in deionized water, is obtained
Water phase；(4) oil is mutually swollen: the carrier oil obtained in step (2) being added in seed polymer microballoon, is stirred evenly, is protected
System temperature 0.5~2h at 14~85 DEG C is held, oil mutually swelling object is obtained；(5) cause solvent swell: taking initiator, emulsifier
And water is mixed, and is added in the oil phase swelling object that step (4) obtain after emulsifying 5~20min；(6) it polymerize: by step (3)
It obtains water phase to be added in oil phase swelling object, is warming up to 75~82 DEG C after mixing evenly, and keep the temperature 10~40h；It is cold after polymerization
But polymer microballoon is obtained to room temperature；(7) it cleans: the polymer microballoon that step (6) obtain being drained, and is cleaned with solvent, is obtained
Obtain partial size monodisperse HbA1C ion-exchange chromatography filler.
4. a kind of partial size monodisperse HbA1C ion-exchange chromatography filler synthetic method according to claim 3, feature exist
In: specific step is as follows for the step (2): the sol vehicle added with oil phase emulsion is transferred to pre-inversion reaction by (2-a)
In device, preliminary polymerization is carried out, generates the mixture comprising oil phase emulsion, sol vehicle and carrier colloidal sol；(2-b) is to step
Mixture in (2-a) is separated, and the first logistics and packet comprising 65~92wt% of mixture in step (2-a) are generated
The second logistics containing surplus；(2-c) adds the first logistics into second reactor, is further polymerize, and the first logistics is caused
Inversion of phases；(2-d) is by the second logistics obtained in the first logistics of inversion of phases and step (2-b) in step (2-c) with 25
~33s-1Shear rate mixed in static mixer, carry out second of inversion of phases, generate gelatinous primary carrier oil phase；
The primary carrier oil that (2-e) obtains step (2-d) mutually carries out melten gel, and filling component and auxiliary components are added, and stirs shape
At carrier oil phase fluid, carrier oil phase is obtained after extrusion.
5. a kind of partial size monodisperse HbA1C ion-exchange chromatography filler synthetic method according to claim 2, feature exist
In: the filling component is polymer filler, is any one in poly- methylpropionate and polystyrene-divinylbenzene
Kind or two kinds of combination.
6. a kind of partial size monodisperse HbA1C ion-exchange chromatography filler synthetic method according to claim 2, feature exist
In: mass fraction shared by the constituent of the auxiliary components and each ingredient is respectively as follows: curing agent: 13~20 parts, expanding material:
11~20 parts, dispersing agent: 10~18 parts, levelling agent: 10~16 parts, stabilizer: 10~15 parts, hydrogen peroxide: 10~15 parts and
Phosphoric acid aluminium liquid: 8~12 parts.
7. a kind of partial size monodisperse HbA1C ion-exchange chromatography filler synthetic method according to claim 3, feature exist
In: the oil phase monomer includes the first monomer, second comonomer and Third monomer；First monomer is styrene, methylbenzene
Ethylene, ethyl styrene, 1-chloro-4-methyl-benzene, methyl acrylate, propyl acrylate, butyl acrylate, methyl methacrylate,
Tetramethacrylate, glycidyl methacrylate, glycidyl acrylate and butyl methacrylate
In any one or any several combination；The second comonomer is acrylamide or hydroxyethyl methacrylate, third
Any one in olefin(e) acid hydroxyl ethyl ester and Methacrylamide or any several combination；The Third monomer is 2- propylene
Amide -2- methyl propane sulfonic acid；The crosslinking agent is divinylbenzene, two propylene benzene, ethyleneglycol dimethyacrylate, triethylene
Acid glyceride, trihydroxy methyl oxypropyl trimethyl acrylic acid, pentaerythritol triacrylate, dimethacrylate,
Triethylene-glycol dimethylacrylate, tetraethylene-glycol dimethylacrylate, 1,3 butylene glycol dimethacrylate
Ester, 1,4- butanediol dimethylacrylate hexamethylene bis Methacrylamide, divinyl phenylmethane, trimethacrylate
Any one in acid glyceride, methylene acrylamide or any several combination；The emulsifier is ionic emulsification
Agent.
8. a kind of partial size monodisperse HbA1C ion-exchange chromatography filler synthetic method according to claim 3, feature exist
In: the stabilizer is polyvinyl alcohol, polyethylene glycol, polyvinylpyrrolidone, hydroxymethyl cellulose, hydroxypropyl cellulose, hydroxyl
In ethyl cellulose, carboxymethyl cellulose, beta-cyclodextrin, Beta-methyl cyclodextrin and hydroxyapatite any one or appoint
It anticipates several combinations.
9. a kind of partial size monodisperse HbA1C ion-exchange chromatography filler synthetic method according to claim 3, feature exist
In: the initiator is benzoyl peroxide, peroxidating bis-isobutyronitrile, azo-bis-iso-dimethyl and azo diisobutyl
Any one in amidine or any several combination.
10. a kind of method for measuring saccharification hemoglobin content, it is characterised in that: use partial size monodisperse HbA1C ion exchange
Chromatograph packing material is measured by efficient liquid phase ion-exchange chromatography.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112742359A (en) * | 2019-10-31 | 2021-05-04 | 昭和电工株式会社 | Method for producing column packing for glycated hemoglobin analysis |
| CN113549183A (en) * | 2021-07-30 | 2021-10-26 | 无锡市凯奥善生物医药科技有限公司 | Preparation method of filler for glycosylated hemoglobin chromatographic column |
| CN113663742A (en) * | 2021-07-30 | 2021-11-19 | 无锡市凯奥善生物医药科技有限公司 | Preparation method of strong cation exchange chromatographic packing for glycosylated hemoglobin |
| CN113804813A (en) * | 2021-09-03 | 2021-12-17 | 江苏月旭新材料科技有限公司 | Preparation method of chromatographic packing for separation of glycosylated hemoglobin |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09127091A (en) * | 1995-11-02 | 1997-05-16 | Sekisui Chem Co Ltd | Method for measuring saccarification hemoglobin |
| CN101229509A (en) * | 2008-02-05 | 2008-07-30 | 南京大学 | Method of synthetizing closely graded polymer high efficiency liquid chromatography stuffing |
| CN101257969A (en) * | 2005-12-02 | 2008-09-03 | 积水化学工业株式会社 | Hydrophilic polymer microparticle, filler for ion exchange liquid chromatography, and method for production of filler for ion exchange liquid chromatography |
| CN101357963A (en) * | 2007-08-01 | 2009-02-04 | 中国科学院大连化学物理研究所 | Preparation method of polystyrene-divinylbenzene microspheres for bilirubin adsorption |
| CN101531701A (en) * | 2008-03-14 | 2009-09-16 | 上海惠中医疗科技有限公司 | Method for preparing glycated haemoglobin separation material |
| CN103435749A (en) * | 2013-08-28 | 2013-12-11 | 江苏博特新材料有限公司 | Preparation of low-salinity and low-viscosity polyacrylamide water-in-water dispersion liquid by employing seed swelling method |
| CN105622852A (en) * | 2015-12-31 | 2016-06-01 | 西北工业大学 | Preparing method for synthesizing shape-controlled anisotropic particles through emulsion polymerization based on asymmetry monomer-swelling non-crosslinking seed particles |
-
2019
- 2019-06-05 CN CN201910487055.2A patent/CN110314664A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09127091A (en) * | 1995-11-02 | 1997-05-16 | Sekisui Chem Co Ltd | Method for measuring saccarification hemoglobin |
| CN101257969A (en) * | 2005-12-02 | 2008-09-03 | 积水化学工业株式会社 | Hydrophilic polymer microparticle, filler for ion exchange liquid chromatography, and method for production of filler for ion exchange liquid chromatography |
| CN101357963A (en) * | 2007-08-01 | 2009-02-04 | 中国科学院大连化学物理研究所 | Preparation method of polystyrene-divinylbenzene microspheres for bilirubin adsorption |
| CN101229509A (en) * | 2008-02-05 | 2008-07-30 | 南京大学 | Method of synthetizing closely graded polymer high efficiency liquid chromatography stuffing |
| CN101531701A (en) * | 2008-03-14 | 2009-09-16 | 上海惠中医疗科技有限公司 | Method for preparing glycated haemoglobin separation material |
| CN103435749A (en) * | 2013-08-28 | 2013-12-11 | 江苏博特新材料有限公司 | Preparation of low-salinity and low-viscosity polyacrylamide water-in-water dispersion liquid by employing seed swelling method |
| CN105622852A (en) * | 2015-12-31 | 2016-06-01 | 西北工业大学 | Preparing method for synthesizing shape-controlled anisotropic particles through emulsion polymerization based on asymmetry monomer-swelling non-crosslinking seed particles |
Non-Patent Citations (3)
| Title |
|---|
| M. OKUBO ET AL.: ""Preparation of micron-size monodisperse polymer particles by seeded polymerization utilizing the dynamic monomer swelling method"", 《COLLOID & POLYMER SCIENCE》 * |
| 包建民等: ""微米级多孔聚苯乙烯－二乙烯苯微球的制备、改性及应用综述"", 《材料导报Ａ：综述篇》 * |
| 王东莎等: ""种子溶胀法制备单分散高交联聚苯乙烯微球"", 《应用化学》 * |
Cited By (8)
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|---|---|---|---|---|
| CN112742359A (en) * | 2019-10-31 | 2021-05-04 | 昭和电工株式会社 | Method for producing column packing for glycated hemoglobin analysis |
| CN112742359B (en) * | 2019-10-31 | 2024-02-06 | 株式会社力森诺科 | Method for producing column packing for glycosylated hemoglobin analysis |
| CN113549183A (en) * | 2021-07-30 | 2021-10-26 | 无锡市凯奥善生物医药科技有限公司 | Preparation method of filler for glycosylated hemoglobin chromatographic column |
| CN113663742A (en) * | 2021-07-30 | 2021-11-19 | 无锡市凯奥善生物医药科技有限公司 | Preparation method of strong cation exchange chromatographic packing for glycosylated hemoglobin |
| CN113549183B (en) * | 2021-07-30 | 2023-08-22 | 无锡市凯奥善生物医药科技有限公司 | Preparation method of packing for glycosylated hemoglobin chromatographic column |
| CN113663742B (en) * | 2021-07-30 | 2024-02-09 | 无锡市凯奥善生物医药科技有限公司 | Preparation method of strong cation exchange chromatographic packing for glycosylated hemoglobin |
| CN113804813A (en) * | 2021-09-03 | 2021-12-17 | 江苏月旭新材料科技有限公司 | Preparation method of chromatographic packing for separation of glycosylated hemoglobin |
| CN113804813B (en) * | 2021-09-03 | 2024-01-26 | 江苏月旭新材料科技有限公司 | Preparation method of chromatographic packing for glycosylated hemoglobin separation |
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