CN110302248A - The method for extracting Chinese toon seed general flavone - Google Patents

The method for extracting Chinese toon seed general flavone Download PDF

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CN110302248A
CN110302248A CN201910553861.5A CN201910553861A CN110302248A CN 110302248 A CN110302248 A CN 110302248A CN 201910553861 A CN201910553861 A CN 201910553861A CN 110302248 A CN110302248 A CN 110302248A
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chinese toon
nades
general flavone
toon seed
hydrogen
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CN110302248B (en
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屈长青
岳少云
董新月
金鑫
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Zhang Junsheng
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Fuyang Normal University
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    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0261—Solvent extraction of solids comprising vibrating mechanisms, e.g. mechanical, acoustical
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Abstract

The present invention provides a kind of methods for extracting Chinese toon seed general flavone, comprising: Chinese toon powder of seeds is mixed in NADES eutectic solvent, is homogenized, centrifugation, filtering, filtrate is purified using AB-8 resin, is eluted with 70%~95% graded ethanol to Chinese toon seed general flavone.Method of the invention improves the efficiency that general flavone is extracted from Chinese toon seed.

Description

The method for extracting Chinese toon seed general flavone
Technical field
The present invention relates to field of medicinal chemistry, extract Chinese toon seed general flavone more particularly, to using eutectic solvent Method.
Background technique
Chinese toon is a kind of traditional Chinese medicine in China also known as spring bud tree, Chinese toon, Ailanthus altissima, root skin, bark, bud, leaf, fruit It can be used as medicine, treatment enteritis, dysentery, scytitis etc. are commonly used in Chinese medicine, it is each that the tissue at each position has been used for treatment The disease of kind various kinds.Research finds Chinese toon seed rich in flavone compound, and bioflavonoid has a variety of pharmacological activity, such as anti- Bacterium, antiviral and antitumor, anti-oxidant, anti-inflammatory, analgesia etc., the especially effect of free radical resisting and anticancer, anti-cancer makes biological species Flavones has broad prospects in the application of medicine, field of food.It is mostly tradition for the extracting method of Chinese toon seed general flavone Water mention, alcohol extracting, water extract-alcohol precipitation, acetone and ethyl acetate extraction, but these traditional methods there are material loss big, yield Not high problem, keeping the extraction of Chinese toon general flavone still mostly is based on crude extract.In consideration of it, the extracting method of Chinese toon general flavone needs It more to study.
Summary of the invention
The object of the present invention is to provide a kind of methods for extracting Chinese toon seed general flavone, to improve the extraction of Chinese toon seed general flavone Efficiency.
The present invention provides a kind of methods for extracting Chinese toon seed general flavone, comprising: it is low that Chinese toon powder of seeds is mixed in NADES In congruent melting solvent, homogeneity is centrifuged, and filtering is purified filtrate using AB-8 resin, with 70%~95% graded ethanol pair Chinese toon seed general flavone is eluted.
In the above-mentioned methods, wherein the choline chloride and 1 that the NADES eutectic solvent is 30% by moisture content, 2- third Glycol composition, wherein the optimum mole ratio of choline chloride and 1,2-PD is 1:4.
In the above-mentioned methods, wherein the mass ratio of the Chinese toon powder of seeds and the NADES eutectic solvent is 1:50.
In the above-mentioned methods, wherein the time of the ultrasound is 15min.
In the above-mentioned methods, wherein the centrifugation is that 4000rpm is centrifuged 25min.
The present invention by different polyalcohols hydrogen donors has screened water-soluble green eutectic solvent, and to extract Chinese toon seed always yellow The technique of ketone obtains the optimum process condition that homogeneity (UAE) method extracts Chinese toon seed general flavone: with the chlorination of moisture content 30% Choline -1,2-PD is extractant, after solid-to-liquid ratio 1:50,35min, is filtered to remove Chinese toon seed residue, contains in filtrate Chinese toon seed general flavone.By compared with traditional extracting method (water (0.0020) and 75% ethyl alcohol (0.0027)), NADES is from perfume (or spice) The more efficient of general flavone is extracted in Chinese toon seed.
Detailed description of the invention
Fig. 1 shows the concentration of rutin and the standard curve of absorbance.
The Chinese toon seed general flavone concentration extracted Fig. 2 shows different NADES, water, ethyl alcohol compares.
Fig. 3 shows infrared spectrogram, wherein A: choline chloride, B:1,2- propylene glycol C:Chcl:1,2- propylene glycol.
Fig. 4 shows the different parameters of extraction process and the relational graph of general flavone concentration, wherein A: hydrogen donor molar ratio, B: moisture content, C: solid-to-liquid ratio, D: extraction time.
Specific embodiment
The following examples can make those skilled in the art that the present invention be more fully understood, but not limit in any way The present invention.Experimental method in following embodiments is unless otherwise specified conventional method.Examination as used in the following examples Material is tested, is conventional biochemical reagent company purchase gained unless otherwise specified.
NADES is hydrogen bond receptor (the Hydrogen Bond by the solid halide salt of certain stoichiometric ratio Accepter, HBA) and the eutectic mixture that is composed of hydrogen bond donor (Hydrogen Bond Donnors), fusing point it is aobvious It writes and is lower than each component materials.NADES is a kind of fully biodegradable and bio-compatible as a kind of novel advanced solvent The material of property.NADES combines the characteristics of ionic liquid, and non-toxic inexpensive, and preparation process is simple, need to only heat and mechanical stirring , it is a kind of novel " green solvent ".The present invention mentions Chinese toon seed total flavonoid using different green eutectic methods It takes, and compares research with traditional experimental method, complete the primary dcreening operation of polyalcohols hydrogen donor, and optimize water solubility The parameter of the extraction Chinese toon seed total flavonoid of NADES.
1 materials and methods
1.1 material
Chinese toon seed is purchased from Fuyang City Taihe County；1,2-PD, glycerine, Isosorbide-5-Nitrae-propylene glycol (are purchased from Wuxi City prospectization Work reagent Co., Ltd) and choline chloride (being purchased from Sinopharm Chemical Reagent Co., Ltd.)；Rutin (is purchased from Wuxi City prospectization Work reagent Co., Ltd)；Other reagents such as ethyl alcohol (> 95%) are that analysis is pure.
1.2 instrument
Ultraviolet specrophotometer: Germany's manufacture；Electronic balance: Shanghai Precision Scientific Apparatus Co., Ltd；Intelligent constant warm water Bath: Beijing Orient Jing Rui development in science and technology Co., Ltd；Rotary Evaporators: Anhui water scientific instrument Co., Ltd；KQ-50B Type ultrasonic cleaner；Fourier infrared spectrograph: Agilent Technologies
1.3 method
1.3.1 the preparation of difference NADESs solvent
The NADES of following table difference alcohols hydrogen donor is placed in conical flask, 65 DEG C of water-baths are simultaneously stirred, until being formed uniform Liquid.After uniform liquid is formed, water is proportionally added into NADES solvent, is placed spare.
Table 1
1.3.2 the extraction of Chinese toon seed general flavone
0.2g Chinese toon powder of seeds is mixed in the above-mentioned NADES solvent of 5mL, ultrasonic 15min, 4 000rpm are centrifuged 25min, Filtering, filtrate is mixed with the aqueous hydrochloric acid solution (mass concentration 50%) of equivalent, and by mixed liquor in 65 DEG C of magnetic agitations 20min, filtering, is discarded precipitating, is then purified using AB-8 resin, finally with 70%~95% graded ethanol by general flavone It is recycled.The Chinese toon seed general flavone of purification and recovery is evaporated under reduced pressure to small size.It places and is used for subsequent experimental.Benefit simultaneously Chinese toon general flavone is extracted with water, 75% alcohol method, extraction conditions are consistent with 1.3.2.
1.3.3 the drafting of standard curve
Precision weighs control substance of Rutin 50mg, sets in 50ml volumetric flask, and scale is dissolved and added to 0.95 ethyl alcohol, and precision is inhaled It takes 10ml to set in 100ml volumetric flask, is diluted to scale with 0.60 ethyl alcohol.Draw 0.5 respectively with liquid-transfering gun, 1.0,2.0,3.0, 4.0,5.0ml rutin comparison liquid (0.1mg/ml) is placed in 10ml volumetric flask, and 0.3ml sodium nitrite is added into each volumetric flask Solution (mass concentration 5%), concussion shakes up, and stands 6min at room temperature；Again plus 0.3ml aluminum nitrate solution (mass concentration is 10%), concussion shakes up, and stands 6min at room temperature；Finally plus 4ml sodium hydroxide solution (mass concentration 4%), volume fraction is used It is settled to scale for 60% ethyl alcohol, shakes up, stands 12min at room temperature, blank reference liquid is made with ethanol reagent, in ultraviolet spectrometry Each group light absorption value (OD value) is measured at photometer 510nm.
1.3.4 the measurement of Chinese toon seed general flavone content
Chinese toon seed general flavone solution 1mL is taken respectively, and the Chinese toon extracted under distinct methods is measured according to method shown in 1.3.3 Seed general flavone content.
1.3.5 the IR Characterization of NADES
According to the NADES that different hydrogen donors and hydrogen acceptor synthesize, there is different physical properties, as conductivity, density, Viscosity, surface tension etc., the primary dcreening operation that this experiment passes through NADES, it is determined that 1,2 propylene glycol carries out it red as hydrogen donor Outer characterization, determines Chcl+1, the synthesis of 2- propylene glycol green eutectic solvent, and the optimization for extracting parameter is tested.
1.3.6 parameter optimization
On the basis of determining best polyalcohols hydrogen donor, screen HBA and HDA molar ratio (1:1,1:2,1:3,1:4, 1:5), the moisture content (10%, 20%, 30%, 40%, 50%) of NADES, NADES and Chinese toona fruit powder liquid-solid ratio (1:25, 1:50,1:100,1:200/mgml) and extraction time (15,25,35,45/min) determine optimal extracting parameter.
1.3.7 data are analyzed
Using statistical method, data are analyzed, data used herein are all with (average ± standard deviation) It indicates, extracts the progress significant difference analysis of Chinese toon seed general flavone concentration using 19.0 couples of difference NADES of SPSS and to alcohol, water.
2 results
The drafting of 2.1 standard curves
Standard curve, regression equation Y=11.035x+0.0047, coefficient R are drawn according to the method for 1.3.32= 0.9987. showing rutin concentration, there are preferable linear relationships with absorbance within the scope of 0~0.06mg/mL, and standard curve is such as Shown in Fig. 1.
2.2 difference NADES and water, alcohol extracting Chinese toon seed general flavone concentration
The Chinese toon seed general flavone concentration that different NADES, water, ethyl alcohol extract is more as shown in Figure 2.Water-soluble NADES is being mentioned It takes in Chinese medicine flavones active component, predominantly polyalcohols hydrogen donor extraction efficiency highest, present invention selection is typical polynary Alcohol is that hydrogen ion donor is compared with and with traditional extracting method, completes the screening of primary dcreening operation condition.
Select different hydrogen donor (polyalcohol, polyacid etc.) that different NADES can be synthesized, the difference of hydrogen donor makes NADES has different physical properties (viscosity, density, surface tension, conductivity etc.), these adjustable physical properties are NADES applies remarkably premise in every field.For NADES is extracted in mentioning ketone active constituent, polyalcohols are as hydrogen The selection of donor has been demonstrated that its Optimality is greater than other hydrogen donors, this experiment choline chloride -1,2 propylene glycol as shown in Figure 2 The extracted general flavone average content 0.0151mg/ml of eutectic solvent and traditional 75% ethyl alcohol 0.0027mg/ml and water 0.0020mg/ml forms significant difference, and choline chloride -1,2 propylene glycol NADES compares other two kinds of polyalcohols NADES extracting concentration is higher, this is because the Hydrogen bonding forces of 1,2 propylene glycol and choline chloride are stronger to form more hydrogen Key.
2.3 infrared characterization chart
Infrared characterization chart is as shown in Figure 3.
This experiment carries out IR Characterization after screening to polyalcohols hydrogen donor, to optimum N ADES, after being used for The optimization of continuous extracting parameter.By the A in Fig. 3 it is found that 3255cm-1For the characteristic absorption peak of choline chloride, wherein 1085cm-1Belong to It is shaken in O-H and C-H, 3384 in the B in Fig. 3cm-1, 2970cm-1, 2932cm-1Peak is shaken for OH-, shakes phase with general 0H- It may be the Chcl:1 in the C in Fig. 3 due to caused by forming hydrogen bond than its peak width, the CH3 vibration peak of 2- propylene glycol is 1402cm-1, It can thus be appreciated that the success of Chcl:1,2- propylene glycol (1:3) IR Characterization.
The optimization of 2.4 NADES extracting parameters
2.4.1 hydrogen donor molar ratio
As green eutectic solvent, the selection of different hydrogen donors is very for the formation of NADES and physical property It is important, hydrogen donor number decide high efficiency and stability that NADES extracts.In general, how much hydrogen donor decides The viscosity of NADES, and as the increase of hydrogen donor can form more hydrogen bonds with hydrogen acceptor and water, increase the stability of solvent, The present invention compares the extraction efficiency of different mol ratio hydrogen donor NADES, can be seen that from the A in Fig. 4 with hydrogen donor mole Increase (1:1-1:3) extraction efficiency of ratio gradually rises, and reaches highest, the decline of 1:4-1:5 extraction efficiency, reason master in 1:3 If caused by excessively causing choline chloride choline and Chinese toon seed total flavonoid binding force to decline due to hydrogen donor.
2.4.2 moisture content
Upon formation, therefore water appropriate is added in the exposed many donor hydroxy chemical groups of meeting to water-soluble NADES More hydrogen bonds can be formed with these groups, so that NADES is more stable.The surface tension of NADES solvent is that moisture content is most heavy The physical property wanted, but excessive water declines the concentration of NADES after combining group's saturation, will affect extraction efficiency, Therefore the different moisture content of NADES is probed into be necessary in extraction.As can be seen that 30% moisture content exists from the B in Fig. 4 The extraction efficiency highest in Chinese toon seed total flavonoid, the decline of 50%, 70% extraction efficiency are extracted, this is because surface tension reduces, Caused by the decline of NADES concentration.
2.4.3 solid-to-liquid ratio
In active ingredient of Chinese herbs extraction, different solid-to-liquid ratios shows different extraction efficiencies, mentions in water-soluble NADES It takes in Chinese toon seed total flavonoid, liquid-solid ratio is too small to will lead to the intermolecular formation hydrogen bond of NADES, thus cannot to the flavones in Chinese toona fruit It adequately extracts, the excessive NADES that will lead to of liquid-solid ratio cannot sufficiently combine Chinese toon seed total flavonoid, so can be seen by the C in Fig. 4 1:50mg/ml out-1Extraction efficiency highest.
2.4.4 extraction time
As shown in the D in Fig. 4, at 35 minutes, water-soluble NADES showed high general flavone recovery rate, from 5-35 minutes As time increases, extraction efficiency increases, and recovery rate declines when to 45 clock, this is because the raising of temperature, NADES's is waved Caused by hair.
The present invention by different polyalcohols hydrogen donors has screened water-soluble green eutectic solvent, and to extract Chinese toon seed always yellow The technique of ketone obtains the optimum process condition that ultrasound-assisted extraction (UAE) method extracts Chinese toon seed general flavone: with moisture content 30% choline chloride -1,2-PD is extractant, and it is always yellow to extract Chinese toon seed by solid-to-liquid ratio 1:50, extraction time 35min Ketone.By the way that compared with traditional extracting method (water (0.0020) and 75% ethyl alcohol (0.0027)), NADES is extracted from Chinese toon seed General flavone it is more efficient.
It will be understood by those skilled in the art that above embodiments are only exemplary embodiments, without departing substantially from spirit of the invention In the case where range, a variety of variations can be carried out, replaced and changed.

Claims (5)

1. a kind of method for extracting Chinese toon seed general flavone, comprising:
Chinese toon powder of seeds is mixed in NADES eutectic solvent, homogeneity, be centrifuged, filtering, by filtrate using AB-8 resin into Row purifying, elutes Chinese toon seed general flavone with 70%~95% graded ethanol.
2. according to the method described in claim 1, wherein, chlorination gallbladder that the NADES eutectic solvent is 30% by moisture content Alkali and 1,2-PD composition, wherein the optimum mole ratio of choline chloride and 1,2-PD is 1:3.
3. according to the method described in claim 1, wherein, the quality of the Chinese toon powder of seeds and the NADES eutectic solvent Than for 1:50.
4. according to the method described in claim 1, wherein, the time of the ultrasound is 15min.
5. according to the method described in claim 1, wherein, the centrifugation is that 4000rpm is centrifuged 25min.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116236520A (en) * 2023-04-28 2023-06-09 南京农业大学 Extraction method of Toona sinensis seed polyphenol

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108354960A (en) * 2018-02-13 2018-08-03 沈阳药科大学 A kind of method of flavonoid glycoside compound in extraction Herba Epimedii
CN108392518A (en) * 2018-03-13 2018-08-14 阜阳师范学院 Chinese toon extract and preparation method thereof and purposes
CN109481502A (en) * 2018-11-02 2019-03-19 阜阳师范学院 A kind of preparation method of Chinese toon extract

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107913308A (en) * 2018-01-08 2018-04-17 陈世伟 A kind of Sichuan chinaberry extract and biomedical uses using root of Beijing euphorbia alkane type triterpenoid as active ingredient

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108354960A (en) * 2018-02-13 2018-08-03 沈阳药科大学 A kind of method of flavonoid glycoside compound in extraction Herba Epimedii
CN108392518A (en) * 2018-03-13 2018-08-14 阜阳师范学院 Chinese toon extract and preparation method thereof and purposes
CN109481502A (en) * 2018-11-02 2019-03-19 阜阳师范学院 A kind of preparation method of Chinese toon extract

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
李常风: "《典型天然药物的化学成分及其研究开发新探》", 31 March 2019, 吉林大学出版社 *
王昌禄等: "香椿老叶中活性物质提取及其抗氧化活性的研究 ", 《农业工程学报》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116236520A (en) * 2023-04-28 2023-06-09 南京农业大学 Extraction method of Toona sinensis seed polyphenol
CN116236520B (en) * 2023-04-28 2024-03-29 南京农业大学 Extraction method of Toona sinensis seed polyphenol

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