CN110301643A - A kind of preparation method of astaxanthin-calcium alginate microsphere - Google Patents
A kind of preparation method of astaxanthin-calcium alginate microsphere Download PDFInfo
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- CN110301643A CN110301643A CN201910706432.7A CN201910706432A CN110301643A CN 110301643 A CN110301643 A CN 110301643A CN 201910706432 A CN201910706432 A CN 201910706432A CN 110301643 A CN110301643 A CN 110301643A
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- Prior art keywords
- astaxanthin
- lecithin
- preparation
- calcium alginate
- follows
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- 239000000648 calcium alginate Substances 0.000 title claims abstract description 30
- 229960002681 calcium alginate Drugs 0.000 title claims abstract description 30
- 239000004005 microsphere Substances 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 claims abstract description 37
- 235000013793 astaxanthin Nutrition 0.000 claims abstract description 37
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 claims abstract description 37
- 229940022405 astaxanthin Drugs 0.000 claims abstract description 37
- 239000001168 astaxanthin Substances 0.000 claims abstract description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 33
- 239000000787 lecithin Substances 0.000 claims abstract description 26
- 229940067606 lecithin Drugs 0.000 claims abstract description 26
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000006185 dispersion Substances 0.000 claims abstract description 14
- 239000004006 olive oil Substances 0.000 claims abstract description 14
- 235000008390 olive oil Nutrition 0.000 claims abstract description 14
- 239000000661 sodium alginate Substances 0.000 claims abstract description 14
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 14
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 14
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims abstract description 12
- 235000010445 lecithin Nutrition 0.000 claims abstract description 12
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 11
- 239000003921 oil Substances 0.000 claims abstract description 8
- 235000019198 oils Nutrition 0.000 claims abstract description 8
- 238000005507 spraying Methods 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims description 25
- 239000000839 emulsion Substances 0.000 claims description 16
- 238000003756 stirring Methods 0.000 claims description 15
- 238000002156 mixing Methods 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 6
- 239000007921 spray Substances 0.000 claims description 4
- 239000001110 calcium chloride Substances 0.000 claims description 2
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 2
- 238000005303 weighing Methods 0.000 claims 3
- 239000006071 cream Substances 0.000 claims 1
- 239000003595 mist Substances 0.000 claims 1
- 210000001072 colon Anatomy 0.000 abstract description 26
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 5
- 230000006378 damage Effects 0.000 abstract 1
- 238000007599 discharging Methods 0.000 abstract 1
- 230000005714 functional activity Effects 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 230000000050 nutritive effect Effects 0.000 abstract 1
- 230000029087 digestion Effects 0.000 description 21
- 238000002133 sample digestion Methods 0.000 description 19
- 210000000813 small intestine Anatomy 0.000 description 12
- 210000000214 mouth Anatomy 0.000 description 8
- 239000012530 fluid Substances 0.000 description 7
- 210000004051 gastric juice Anatomy 0.000 description 7
- 238000004088 simulation Methods 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 230000008859 change Effects 0.000 description 5
- 230000000968 intestinal effect Effects 0.000 description 5
- 210000002784 stomach Anatomy 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 230000000112 colonic effect Effects 0.000 description 4
- 238000000879 optical micrograph Methods 0.000 description 4
- -1 phosphatide Substances 0.000 description 4
- 239000012467 final product Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 150000003904 phospholipids Chemical class 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 241000238557 Decapoda Species 0.000 description 2
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 2
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000005660 chlorination reaction Methods 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000006210 lotion Substances 0.000 description 2
- 238000000399 optical microscopy Methods 0.000 description 2
- 230000001175 peptic effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000019155 vitamin A Nutrition 0.000 description 2
- 239000011719 vitamin A Substances 0.000 description 2
- 229940045997 vitamin a Drugs 0.000 description 2
- 208000019399 Colonic disease Diseases 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 208000005016 Intestinal Neoplasms Diseases 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 229930003316 Vitamin D Natural products 0.000 description 1
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 238000000889 atomisation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000013265 extended release Methods 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000021323 fish oil Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 201000002313 intestinal cancer Diseases 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 239000011806 microball Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- VOFUROIFQGPCGE-UHFFFAOYSA-N nile red Chemical compound C1=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4OC3=CC(=O)C2=C1 VOFUROIFQGPCGE-UHFFFAOYSA-N 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 210000000664 rectum Anatomy 0.000 description 1
- 239000001044 red dye Substances 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 150000003710 vitamin D derivatives Chemical class 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940046008 vitamin d Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/20—Agglomerating; Granulating; Tabletting
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/12—Ketones
- A61K31/122—Ketones having the oxygen directly attached to a ring, e.g. quinones, vitamin K1, anthralin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1629—Organic macromolecular compounds
- A61K9/1652—Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Medicinal Preparation (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
It is the astaxanthin-calcium alginate microsphere preparation method for discharging target spot that the invention discloses a kind of with colon, is prepared into oily phase comprising steps of astaxanthin is dissolved in olive oil；Lecithin is dissolved in water, is configured to water phase；The water phase is mutually mixed with the oil, sodium alginate is added after high speed dispersion, homogeneous and obtains mixed liquor；The mixed liquor is instilled in calcium chloride solution in spraying mode, obtains astaxanthin-calcium alginate microsphere.The present invention is that wall material embeds astaxanthin using sodium alginate, obtain a kind of astaxanthin-calcium alginate microsphere, both stability of the astaxanthin under condition of storage can have been enhanced, it again can destruction to avoid upper digestive tract to astaxanthin, it realizes Colon-specific release, there is better nutritive value and functional activity to colon.
Description
Technical field
The present invention relates to food processing technology fields, and in particular to a kind of using colon as astaxanthin-calcium alginate of target spot
The preparation method of microballoon.
Background technique
Fat-soluble nutrients substance refers to that one kind can be dissolved in fat, and then by human body intake, digestion, the object absorbed
Matter, common fat-soluble nutrients substance include fish oil, algae oil, phosphatide, vitamin A, vitamin D, vitamin E etc..Astaxanthin is
A kind of fat-soluble carotenoid nutrient, is the main source of internal vitamin A, while also having inoxidizability, prevention
Cancer, strengthen immunity improve eyesight, anti-aging and other effects.
In recent years, astaxanthin is more and more paid attention to as a kind of nutrition and drug ingedient by consumer, but astaxanthin
It is water-soluble poor, limit its application in the food industry.In addition, the double bond on astaxanthin molecular structure makes it vulnerable to oxidation
The influence of a variety of environmental factors such as agent, light, heat, acid and be destroyed, cause unstable product quality.Therefore, astaxanthin is being used for
Before functional food, some form of protection is needed.Common method includes lotion, nanoparticle, liposome etc., but above-mentioned load
Fortune system all by by small molecule object small intestine stablize discharge for the purpose of.Astaxanthin is to the colons class disease such as intestinal inflammatory, intestinal cancer
Disease has good protective action, but divides in the absorption site of astaxanthin and the otherness of action target spot and astaxanthin transport process
The change of minor structure is applied to exert a certain influence.Therefore, construct it is a kind of can make astaxanthin colon site stabilization release
The carrier put is tied to form as a kind of urgent demand.
Summary of the invention
The purpose of the present invention is overcoming the prior art, preparing a kind of can be such that astaxanthin stablizes in colon to discharge and inhale
The carrying system of receipts.
In order to achieve the above object, the present invention provides a kind of preparation method of astaxanthin-calcium alginate microsphere, including step
It is rapid:
S1, it prepares oily phase: taking astaxanthin to be dissolved in olive oil, be configured to oily phase；The weight of the astaxanthin and olive oil
Measure volume ratio are as follows: 1:90~1:110g/ml；
S2, preparation water phase: it takes lecithin to be dissolved in the water, is configured to water phase；The solid-liquid ratio of the lecithin and water are as follows: 1:
15~1:35g/ml
S3, mixing: by oil described in step S1, mutually 1:24~1:9 is mixed by volume with water phase described in step S2,13000~
15000rpm/min 2~4min of high speed dispersion, obtains mixed liquor A；
S4, homogeneous: it by mixed liquor A described in step S3 high-pressure homogeneous 2~5 times under 9000~10000psi pressure, obtains
Stable astaxanthin-lecithin emulsions；
S5, dispersion: being added sodium alginate for astaxanthin described in step S4-lecithin emulsions, 20~25 DEG C, stirring, sufficiently
After mixing, 14000~16000rpm/min, 2~3min of high speed dispersion obtains mixed liquid B；The sodium alginate and astaxanthin-ovum
The w/v of phospholipid emulsion is 1:50~1:25g/ml；
S6, microballoon preparation: mixed liquid B described in step S5 is atomized to the calcium chloride for instilling 5~10g/ml with spraying mode
It in solution, is stirred and evenly mixed at 20~25 DEG C, obtains final product astaxanthin-calcium alginate microsphere；The mixed liquid B and calcium chloride solution
Volume ratio be 1:100~1:120.
It under preferred embodiment, is stirred described in step S5 specifically: 250~350rpm stirs 30~40min.
Under preferred embodiment, spraying droplet size described in step S6 are as follows: 0.1~10 μm；The stirring specifically: 250~
350rpm stirs 30~40min.
Under preferred embodiment, the preparation method of the astaxanthin-calcium alginate microsphere, comprising steps of
S1, it prepares oily phase: taking astaxanthin to be dissolved in olive oil, be configured to oily phase；The weight of the astaxanthin and olive oil
Measure volume ratio are as follows: 1:100g/ml；
S2, preparation water phase: it takes lecithin to be dissolved in the water, is configured to water phase；The solid-liquid ratio of the lecithin and water are as follows: 1:
20g/ml
S3, mixing: by oil described in step S1, mutually 7:93 is mixed by volume with water phase described in step S2,15000rpm/min
High speed dispersion 2min, obtains mixed liquor A；
S4, homogeneous: by mixed liquor A described in step S3 high-pressure homogeneous 5 times under 10000psi pressure, it is green to obtain stable shrimp
Element-lecithin emulsions；
S5, dispersion: being added sodium alginate for astaxanthin described in step S4-lecithin emulsions, at 25 DEG C, 300rpm stirring
30min, after mixing well, 15000rpm/min high speed dispersion 2min obtains mixed liquid B；The sodium alginate and astaxanthin-ovum
The w/v of phospholipid emulsion is 3:100g/ml；
S6, microballoon preparation: mixed liquid B described in step S5 is atomized to the chlorination for instilling 7g/ml with watering can in spraying mode
In calcium solution, at 25 DEG C, 300rpm stir 30min, obtain final product astaxanthin-calcium alginate microsphere；The watering can sprays droplet
Size are as follows: 0.1~10 μm；The mixed liquid B and the volume ratio of calcium chloride solution are 1:100.
The beneficial effects of the present invention are:
After mixing by astaxanthin-lecithin emulsions and sodium alginate, it is dispersed into spray pattern micron-sized
Drop reacts the astaxanthin-calcium alginate microsphere to form that partial size is micron level with calcium chloride solution, can both enhance astaxanthin and exist
Stability under condition of storage and in upper digestive tract transport process, and can be discharged by the microbial lytic of colon, make astaxanthin
It discharges and absorbs in colon.
Of the invention preparation method is simple, and the microballoon produced has preferable stability, can be used for astaxanthin
Stablize storage and intracorporal Targeting delivery, there is better effect to colonic disease.
Detailed description of the invention
Astaxanthin-calcium alginate microsphere prepared by Fig. 1 optical microphotograph sem observation embodiment of the present invention 1 is after salt ion is handled
State, scale bar be Bar=20 μm.
Fig. 2 is astaxanthin-calcium alginate microsphere optical microscope prepared by the embodiment of the present invention 1, scale bar Bar
=20 μm.
Fig. 3 is the optical microphotograph sem observation figure of oral digestion sample described in the embodiment of the present invention 2, and scale bar is Bar=20 μ
m。
Fig. 4 is the optical microphotograph sem observation figure of peptic digest sample described in the embodiment of the present invention 2, and scale bar is Bar=20 μm.
Fig. 5 is the optical microphotograph sem observation figure of small intestine sample digestion described in the embodiment of the present invention 2, and scale bar is Bar=20 μ
m。
Fig. 6 is astaxanthin-calcium alginate microsphere fluorescence microscope figure prepared by the embodiment of the present invention 1, observes multiple
For 200x.
Fig. 7 is the fluorescence microscope figure of oral digestion sample described in the embodiment of the present invention 2, and observation multiple is 200x.
Fig. 8 is the fluorescence microscope figure of peptic digest sample described in the embodiment of the present invention 2, and observation multiple is 200x.
Fig. 9 is the fluorescence microscope figure of small intestine sample digestion described in the embodiment of the present invention 2, and observation multiple is 200x.
Figure 10 is the fluorescence microscope figure for the colon sample digestion that the embodiment of the present invention 2 digests 0h, and observation multiple is
400x。
Figure 11 is the fluorescence microscope figure for the colon sample digestion that the embodiment of the present invention 2 digests 3h, and observation multiple is
400x。
Figure 12 is the fluorescence microscope figure for the colon sample digestion that the embodiment of the present invention 2 digests 6h, and observation multiple is
400x。
Figure 13 is the fluorescence microscope figure of the colon sample digestion of the digestion of the embodiment of the present invention 2 for 24 hours, and observation multiple is
400x。
Specific embodiment
Below in conjunction with drawings and examples, a specific embodiment of the invention is described in more details, so as to energy
The advantages of more fully understanding the solution of the present invention and its various aspects.However, specific embodiments described below and implementation
Example is for illustrative purposes only, rather than limiting the invention.
Technical problem to be solved by the present invention lies in: how to prepare it is a kind of can make astaxanthin colon stablize discharge and inhale
The carrying system of receipts.
Technical problems to be solved needed for the present invention are achieved through the following technical solutions: one kind is using colon as target spot
Astaxanthin-calcium alginate microsphere preparation method, which is characterized in that method includes the following steps: (1) by astaxanthin according to
1:100 (w/v) is dissolved in olive oil, and it is mutually stand-by to be configured to oil；(2) lecithin is dissolved in deionized water according to 1:20 (w/v)
In, it is stand-by to be configured to water phase；(3) water phase is mutually mixed with oily with the ratio of 93:7,15000rpm/min high speed dispersion 2min；
(4) stable astaxanthin-lecithin emulsions are obtained after 10000psi high-pressure homogeneous 5 times；(5) 3% (w/v) is added in lotion again
Sodium alginate, 25 DEG C of stirring 30min mix well, 15000rpm/min high speed dispersion 2min；(6) solution is with pressure atomization
Mode instill in the calcium chloride solution of 7% (w/v), 30min is stirred at room temperature, stable astaxanthin-calcium alginate microsphere is made.
Embodiment 1
S1, it prepares oily phase: taking astaxanthin to be dissolved in olive oil, be configured to oily phase；The weight of the astaxanthin and olive oil
Measure volume ratio are as follows: 1:100g/ml；
S2, preparation water phase: it takes lecithin to be dissolved in the water, is configured to water phase；The solid-liquid ratio of the lecithin and water are as follows: 1:
20g/ml
S3, mixing: by oil described in step S1, mutually 7:93 is mixed by volume with water phase described in step S2,15000rpm/min
High speed dispersion 2min, obtains mixed liquor A；
S4, homogeneous: by mixed liquor A described in step S3 high-pressure homogeneous 5 times under 10000psi pressure, it is green to obtain stable shrimp
Element-lecithin emulsions；
S5, dispersion: being added sodium alginate for astaxanthin described in step S4-lecithin emulsions, at 25 DEG C, 300rpm stirring
30min, after mixing well, 15000rpm/min high speed dispersion 2min obtains mixed liquid B；The sodium alginate and astaxanthin-ovum
The w/v of phospholipid emulsion is 3:100g/ml；
S6, microballoon preparation: mixed liquid B described in step S5 is atomized to the chlorination for instilling 7g/ml with watering can in spraying mode
In calcium solution, at 25 DEG C, 300rpm stir 30min, obtain final product astaxanthin-calcium alginate microsphere；The watering can sprays droplet
Size are as follows: 0.1~10 μm；The mixed liquid B and the volume ratio of calcium chloride solution are 1:100.
Embodiment 2
(1) Stability Determination.
Salt ion processing: configuring the NaCl solution of 0.5mol/L, and 1 products therefrom astaxanthin of embodiment-calcium alginate is micro-
Ball is added in NaCl solution with the ratio of volume ratio 1:1, surveys partial size after reacting 30min.And observed with optical microscopy,
As a result as shown in Figure 1.
(2) configuration of digestive juice
Oral fluid: after gargling, the saliva flowed out naturally is as oral fluid.
Gastric juice: 10g pepsin (15000U) is dissolved in 16.4mL dilute hydrochloric acid (10%, v/v), is diluted with water to
1000mL adjusts pH to 2；
Intestinal fluid: 6.8g KH2PO4It is dissolved in 1000mL distilled water with 10g trypsase (>=180units), adjusts pH
To 6.8；
Colonic fluid: taking 1g fresh excreta to be put into 5mL centrifuge tube, 2mL PBS solution is added, 4 DEG C of standing 5min, be vortexed shake
It is complete to swing dissolution；It is centrifuged (1000rpm, 5min), takes 0.5mL supernatant to be added in the GMM culture medium of 5mL sterilizing, as colon
Liquid；Wherein, the composition of GMM culture medium is as shown in Table 1:
The formula of 1 GMM of table
The vitamin mixtures:MD-VSTM, mineral mixture:MD-TMSTM。
(3) simulation digestion
Oral cavity simulation: astaxanthin-calcium alginate microsphere prepared by embodiment 1 is with the ratio of volume ratio 1:1 and the mouth
The mixing of chamber liquid, 120rpm rocks 3h under the conditions of 37 DEG C, obtains oral digestion sample；
Stomach simulation: the oral digestion sample is mixed for the ratio of 1:1 with gastric juice with volume ratio, 37 DEG C of reaction 3h are obtained
To Gastric juice digestion sample；
Small intestine simulation: the ratio that the Gastric juice digestion sample is 1:1 by volume is mixed with intestinal fluid, at 37 DEG C respectively
Reaction for 24 hours, obtains small intestine sample digestion；
Colon simulation: the ratio that the small intestine sample digestion is 1:1 by volume is mixed with colonic fluid, 37 DEG C of anaerobism items
It is reacted under part for 24 hours, obtains colon sample digestion.
Oral cavity sample digestion, Gastric juice digestion sample and small intestine sample digestion are observed with optical microscopy, as a result such as
Shown in Fig. 2~Fig. 5.
Dyeing: respectively by oral digestion sample, Gastric juice digestion sample, small intestine sample digestion and colon sample digestion and 1mg/
ML Nile red dye liquor reacts one minute, is observed under 543nm exciting light with fluorescence microscope；Wherein, the colon sample digestion
Respectively colon simulation digestion 0h, 3h, 6h and for 24 hours after colon sample digestion；Wherein, the colon of the colon simulation digestion 0h
Sample digestion is by the small intestine sample digestion and the mixed sample of colonic fluid 1:1 progress by volume.As a result such as Fig. 6
Shown in~Figure 13.
As shown in Figure 1, astaxanthin-calcium alginate microsphere prepared by embodiment 1 is after salt ion is handled, form size is not
Significant change occurs.Fig. 2 to Fig. 5 is for astaxanthin-calcium alginate microsphere of the preparation of the embodiment of the present invention 1 and its by oral cavity, stomach
And the postdigestive micro- sem observation picture of small intestine.The experimental results showed that 0.5~5 micron of the diameter of microballoon, and pass through upper digestive tract
Digestion after (i.e. after oral cavity, stomach and small intestinal digestion), there is no substantially change for appearance.In addition, Fig. 6 to Fig. 9 is
The embodiment of the present invention 1 prepare astaxanthin-calcium alginate microsphere is unprocessed and the postdigestive release feelings of direct oral cavity, stomach, small intestine
Condition, fluorescence microscope as the result is shown microballoon after upper digestive tract digests (i.e. after oral cavity, stomach and small intestinal digestion), disappear in oral cavity
Change the release for not detecting apparent olive oil in sample, Gastric juice digestion sample and small intestine sample digestion, shows the microballoon
The digestion of tolerable upper digestive tract.Figure 10 to Figure 13 is astaxanthin-calcium alginate microsphere of the preparation of the embodiment of the present invention 1 in colon
Digested 0h, 3h, 6h, for 24 hours after release conditions, fluorescence microscope as the result is shown microballoon per rectum liquid digestion after, digestion 3h,
The release of apparent astaxanthin-containing olive oil is detected in 6h, colon sample digestion for 24 hours, and extended release at any time is more
Obviously, show that the microballoon can be destroyed in colonic environment, and then release the astaxanthin of carrying.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art within the technical scope of the present disclosure, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (5)
1. a kind of preparation method of astaxanthin-calcium alginate microsphere, which is characterized in that comprising steps of
S1, it prepares oily phase: taking astaxanthin to be dissolved in olive oil, be configured to oily phase；The weighing body of the astaxanthin and olive oil
Product ratio are as follows: 1:90~1:110g/ml；
S2, preparation water phase: it takes lecithin to be dissolved in the water, is configured to water phase；The solid-liquid ratio of the lecithin and water are as follows: 1:15~
1:35g/ml
S3, mixing: by oil described in step S1, mutually 1:24~1:9 is mixed by volume with water phase described in step S2,13000~
15000rpm/min disperses 2~4min, obtains mixed liquor A；
S4, homogeneous: by mixed liquor A described in step S3 at 9000~10000psi homogeneous 2~5 times, astaxanthin-lecithin cream is obtained
Liquid；
S5, dispersion: being added sodium alginate for astaxanthin described in step S4-lecithin emulsions, and 20~25 DEG C stir and evenly mix, and 14000
~16000rpm/min disperses 2~3min, obtains mixed liquid B；The sodium alginate and astaxanthin-lecithin emulsions weighing body
Product is than being 1:50~1:25g/ml；
S6, microballoon preparation: mixed liquid B described in step S5 is atomized to the calcium chloride solution for instilling 5~10g/ml with spraying mode
In, 20~25 DEG C stir and evenly mix, and obtain astaxanthin-calcium alginate microsphere；The mixed liquid B and the volume ratio of calcium chloride solution are 1:
100~1:120.
2. astaxanthin-calcium alginate microsphere preparation method according to claim 1, which is characterized in that stirred described in step S5
The parameter mixed are as follows: 250~350rpm, 30~40min of stirring.
3. astaxanthin-calcium alginate microsphere preparation method according to claim 1, which is characterized in that sprayed described in step S6
The droplet size of mist are as follows: 0.1~10 μm.
4. astaxanthin-calcium alginate microsphere preparation method according to claim 1, which is characterized in that stirred described in step S6
The parameter mixed are as follows: 250~350rpm, 30~40min of stirring.
5. astaxanthin-calcium alginate microsphere preparation method according to claim 1, which is characterized in that comprising steps of
S1, it prepares oily phase: taking astaxanthin to be dissolved in olive oil, be configured to oily phase；The weighing body of the astaxanthin and olive oil
Product ratio are as follows: 1:100g/ml；
S2, preparation water phase: it takes lecithin to be dissolved in the water, is configured to water phase；The solid-liquid ratio of the lecithin and water are as follows: 1:20g/
ml；
S3, mixing: oil described in step S1 is mutually dispersed with the 7:93 mixing by volume of water phase described in step S2,15000rpm/min
2min obtains mixed liquor A；
S4, homogeneous: by mixed liquor A described in step S3 at 10000psi homogeneous 5 times, astaxanthin-lecithin emulsions are obtained；
S5, dispersion: being added sodium alginate for astaxanthin described in step S4-lecithin emulsions, 25 DEG C, 300rpm stir 30min,
15000rpm/min disperses 2min, obtains mixed liquid B；The sodium alginate and astaxanthin-lecithin emulsions w/v
For 3:100g/ml；
S6, microballoon preparation: the calcium chloride that mixed liquid B described in step S5 is atomized instillation 7g/ml in spraying mode with watering can is molten
In liquid, 25 DEG C, 300rpm stirring 30min obtain astaxanthin-calcium alginate microsphere；The watering can sprays droplet size are as follows: 0.1~
10μm；The mixed liquid B and the volume ratio of calcium chloride solution are 1:100.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111184870A (en) * | 2019-10-23 | 2020-05-22 | 浙江海洋大学 | Preparation method of astaxanthin transporter with gastric juice digestion resistance |
| CN112931907A (en) * | 2021-04-06 | 2021-06-11 | 大连工业大学 | Preparation method of microspheres for improving astaxanthin release rate based on isolated whey protein |
| CN113170885A (en) * | 2021-04-06 | 2021-07-27 | 大连工业大学 | Preparation method of microspheres for improving astaxanthin release rate based on fucoidin |
| CN115252575A (en) * | 2022-08-01 | 2022-11-01 | 中国科学院天津工业生物技术研究所 | Oil gel microsphere with intestinal tract targeted release function and preparation method thereof |
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|---|---|---|---|---|
| CN104473878A (en) * | 2014-11-20 | 2015-04-01 | 中国海洋大学 | Method for preparing high-aqueous-dispersion astaxanthin ester microspheres |
| CN104856963A (en) * | 2015-05-26 | 2015-08-26 | 大连理工大学 | Astaxanthin-sodium alginate sustained release microspheres as well as preparation method and application thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104473878A (en) * | 2014-11-20 | 2015-04-01 | 中国海洋大学 | Method for preparing high-aqueous-dispersion astaxanthin ester microspheres |
| CN104856963A (en) * | 2015-05-26 | 2015-08-26 | 大连理工大学 | Astaxanthin-sodium alginate sustained release microspheres as well as preparation method and application thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111184870A (en) * | 2019-10-23 | 2020-05-22 | 浙江海洋大学 | Preparation method of astaxanthin transporter with gastric juice digestion resistance |
| CN112931907A (en) * | 2021-04-06 | 2021-06-11 | 大连工业大学 | Preparation method of microspheres for improving astaxanthin release rate based on isolated whey protein |
| CN113170885A (en) * | 2021-04-06 | 2021-07-27 | 大连工业大学 | Preparation method of microspheres for improving astaxanthin release rate based on fucoidin |
| CN115252575A (en) * | 2022-08-01 | 2022-11-01 | 中国科学院天津工业生物技术研究所 | Oil gel microsphere with intestinal tract targeted release function and preparation method thereof |
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