CN110301549A - A kind of health-care feed and preparation method thereof enhancing Micropterus salmoides function of intestinal canal and test method - Google Patents
A kind of health-care feed and preparation method thereof enhancing Micropterus salmoides function of intestinal canal and test method Download PDFInfo
- Publication number
- CN110301549A CN110301549A CN201910587902.2A CN201910587902A CN110301549A CN 110301549 A CN110301549 A CN 110301549A CN 201910587902 A CN201910587902 A CN 201910587902A CN 110301549 A CN110301549 A CN 110301549A
- Authority
- CN
- China
- Prior art keywords
- parts
- health
- care feed
- test
- micropterus salmoides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The present invention relates to a kind of health-care feed for enhancing Micropterus salmoides function of intestinal canal and preparation method thereof and test methods; the forage protein of the health-care feed keeps 46%; it is calculated according to 100 parts of total raw material, the health-care feed contains 5-7 parts of yeast hydrolyate, 0.5-2 parts of Pulvis Fellis Suis, 0.5-3 parts of high temperature resistant digestive ferment, 1-3 parts of butyrin, 0.5-2 parts of origanum oil, 0.5-2 parts of taurine, 0.5-2 parts of curcumin, 1-3 parts of high temperature resistant bacteria preparation, 0.5-1.5 parts of multidimensional and 4-8 parts of high protein fermentation material.Feed of the invention is in addition to there is normal diet nutrient balance and abundant; also specially strengthen and promotes intestinal wall reparation; inhibit harmful intestinal tract bacteria; promote the functions such as digestion; it mixes and feeds with normal diet collocation; to reduce the generation of enterogastritis, mitigate the labour of daily shield intestines product spice, and can promote to grow like that with normal diet.
Description
Technical field
The present invention relates to a kind of Micropterus salmoides feed, especially a kind of health-care feed for enhancing Micropterus salmoides function of intestinal canal and
Preparation method and test method.
Background technique
Currently, the feed of Micropterus salmoides (also known as largemouth bass) has tended to mature, and successfully substitute traditional frost fish
It is fresh to become staple food, reduce the propagation of chilled middle pathogen.However, the function of intestinal canal of Micropterus salmoides is still complete without domestication,
To adapt to high-intensitive feedstuff feeding, and feed pursues fast-growth, and the nutriment of the inside is very rich, needs to employ big
The digestive function of amount, by high-intensitive digestion and absorption, the frequency that intestinal wall cell is updated the replacement increases enteron aisle, once it updates
Speed does not catch up with depletion rate, is easy to keep intestinal wall impaired, causes enterogastritis.The characteristic of largemouth bass enteron aisle is that intestinal wall is thin, and enteron aisle is short,
And central immune cells surround intestinal wall, long-term enterogastritis certainly will make body lose protection, in water, the pathogen meeting in feed
Enter easily in vivo, also decline is immunized because resisting for a long time with enteric pathogenic bacteria in immune system, and eventually leading to bacterium enters body, outburst
Ripe body, determines a series of problems, such as body at rotten body.So intestinal health is most important in addition to ensureing digestion and absorption and aquatic animal
Immune organ.Enhance function of intestinal canal, improve and be immunized, becomes one of the effective means of prevention Micropterus salmoides various bacterial disease.
Summary of the invention
A purpose of the present invention is that providing a kind of energy promotion reparation Micropterus salmoides intestinal wall, enhance function of intestinal canal, and improve
The health-care feed of immune enhancing Micropterus salmoides function of intestinal canal.
A purpose of the invention is achieved in that
The forage protein of a kind of health-care feed enhancing Micropterus salmoides function of intestinal canal, health-care feed keeps 46%, characterized in that presses
It is calculated according to 100 parts of total raw material, the health-care feed contains 5-7 parts of yeast hydrolyate, 0.5-2 parts of Pulvis Fellis Suis, high temperature resistant digestive ferment
0.5-3 parts, 1-3 parts of butyrin, 0.5-2 parts of origanum oil, 0.5-2 parts of taurine, 0.5-2 parts of curcumin, high temperature resistant bacterium
1-3 parts of preparation, 0.5-1.5 parts of multidimensional and 4-8 parts of high protein fermentation material.
Pulvis Fellis Suis is a kind of Chinese medicine, it derives from animal pig, is the dry bile of this animal, is not related to artificial chemistry
Ingredient does not have an adverse effect to Micropterus salmoides.Pulvis Fellis Suis has certain fishy smell, plays attractant effect, and can enter liver
Through with gallbladder channel and large intestine channel, therefore, drug effect can reach the liver and gallbladder intestines of Micropterus salmoides.
Be avoided that in feed preparation process using high temperature resistant digestive ferment, high temperature resistant bacteria preparation influenced because of high temperature its activity at
Point, enhance the digestic property of Micropterus salmoides.
Origanum oil has anti-corrosion, antibacterial, antiviral, anti-inflammatory, antimycotic and other effects, improves the resistance of Micropterus salmoides, and
And inhibit the proliferation of harmful intestinal tract bacteria, reduce enteritis disease incidence.
Taurine has enhancing immune function, cholagogue, strong liver, removing toxic substances and other effects.
Curcumin has anti-oxidant, cholagogue, anti-inflammatory and other effects.
Yeast hydrolyate contains a large amount of zymosans and nucleic acid, can effectively optimize intestinal flora ratio, promotes intestinal wall reparation,
It safeguards intestinal health, reduces the generation of enteritis.Immune due to Micropterus salmoides is concentrated mainly near enteron aisle, the weight as body
Barrier is wanted, integrality is extremely important, reduction being immunized great raising body of enteritis morbidity.
Tributyrin has fabulous intestinal wall repair function, and can be anti-oxidant, improves immune and other effects.
In conclusion therefore playing rush containing protection liver, enteron aisle, gallbladder and digestive function in above-mentioned formula
Multiple Micropterus salmoides intestinal wall is studied, function of intestinal canal is enhanced, reduces enteritis disease incidence, and improve immune effect.
The purpose of the present invention can also be solved using following technical measures:
As more specific scheme, the health-care feed also contains the molten slurry of fish meal, starch, yeast extract, fish and meat meal tankage.Fish meal,
The molten slurry of starch, yeast extract, fish and meat meal tankage belong to the nutrition of Micropterus salmoides, foodstuff of allaying one's hunger.
As further embodiment, 66 parts of fish meal, 8 parts of starch, 5-7 parts of yeast hydrolyate, pig gall in the health-care feed
0.5-2 parts of powder, 0.5-3 parts of high temperature resistant digestive ferment, 1-3 parts of butyrin, 0.5-2 parts of origanum oil, 0.5-2 parts of taurine,
0.5-2 parts of curcumin, 1-3 parts of high temperature resistant bacteria preparation, 0.5-1.5 parts of multidimensional, 4-8 parts of high protein fermentation material, 2 parts of yeast extract, fish
4 parts and 1 part of meat meal tankage of molten slurry, adds up to totally 100 parts.
As further embodiment, 66 parts of fish meal, 8 parts of starch, 5 parts of yeast hydrolyate, Pulvis Fellis Suis 1 in the health-care feed
Part, 1 part of high temperature resistant digestive ferment, 2 parts of butyrin, 1 part of origanum oil, 1 part of taurine, 1 part of curcumin, high temperature resistant bacteria preparation
1.5 parts, 0.5 part of multidimensional, 5 parts of high protein fermentation material, 2 parts of yeast extract, molten 4 parts and 1 part of meat meal tankage, totally 100 parts of the slurry of fish.
As further embodiment, the health-care feed is particulate material (No. 1-6), grain diameter 1.5-8.0mm.It protects
The partial size of strong feed is generally made as big as normal diet depending on common Micropterus salmoides feed (normal diet)
Small shape, in order to which health-care feed is mixed with normal diet.
Another object of the present invention is to provide a kind of health-care feeds of the enhancing Micropterus salmoides function of intestinal canal of handling ease
Preparation process.
It is another object of the present invention to what is be achieved:
A kind of preparation process for the health-care feed enhancing Micropterus salmoides function of intestinal canal, characterized in that preparation step is as follows:
Step 1 mixes complete after accurately weighing the raw material；
Step 2, it is quenched by injection vapor；
Step 3 is fabricated to the model similar with normal diet；
Step 4, air-cooled drying.
Another object of the present invention is to provide a kind of enhancing Micropterus salmoides intestines of exact test health-care feed feeding effect
The test method of the health-care feed of road function.
A further object of the present invention is achieved in that
A kind of test method for the health-care feed enhancing Micropterus salmoides function of intestinal canal, characterized in that similar in test selection specification,
With a batch of Micropterus salmoides N tail, it is randomly divided into the processing of X group, Y repetition of every group of processing is each to repeat N/(XY) tail, N X, Y
Positive several times；Wherein, one group is control group, and the Micropterus salmoides of control group is fed using normal diet, and other groups are test group, respectively
The health-care feed that different proportion is respectively adopted in the Micropterus salmoides of test group is fed；60 days experimental periods of test, pre-feeding period 7
It thoroughly cleans test cylinder (jar) and carries out pasteurised completely before testing, test Micropterus salmoides raising is in test cylinder (jar), by the 3% of weight
Feed is fed, 2 times a day, feeding the time is 09: 00 and 17: 00, fishes for residual feed within 2 hours after feeding, is dried, weighing；Examination
The food ration and dead fish quantity of Micropterus salmoides are recorded during testing daily, after the test tail bone venous blood collection.
A further object of the present invention can also be solved using following technical measures:
As more specific scheme, the N is 240, X 2, and Y 3, test group is one group, and the ratio of health-care feed is
100%。
As further embodiment, the N is 360, X 3, and Y 3, test group is two groups, and two groups of test groups are respectively the
One test group and the second test group, the ratio of health-care feed and normal diet is 1:4 in the first test group, is protected in the second test group
The ratio of strong feed and normal diet is 1:3.
As further embodiment, the forage protein of the normal diet keeps 46%, wherein 66 parts of fish meal, 8 parts of starch,
Molten 4 parts of the slurry of 0.1 part of multidimensional, 2 parts of yeast extract, fish, 1 part of meat meal tankage, 10 parts of dregs of beans, 3 parts of corn flour and 5.9 parts of wheat, totally 100
Part.
Beneficial effects of the present invention are as follows:
(1) for the present invention in order to solve to cause Micropterus salmoides intestinal wall impaired because feedstuff feeding is big, function of intestinal canal decline causes stomach
The problems such as inflammation, immune function decline, induction dead fish, ripe body, rotten body, a kind of health-care feed of the invention, the feed is in addition to having
Normal diet nutrient balance and abundant feature are also specially strengthened and promote intestinal wall reparation, and harmful intestinal tract bacteria is inhibited, and promote digestion etc.
Function mixes with normal diet collocation and feeds, to reduce the generation of enterogastritis, mitigates the labour of daily shield intestines product spice
Power, and can promote to grow like that with normal diet.
(2) present invention is by adding yeast hydrolyate as attractant substance, caused by can overcome the disadvantages that the addition of other factors
Palatability problems.
(3) present invention is by being properly added yeast hydrolyate, Pulvis Fellis Suis, high temperature resistant digestive ferment, butyrin, wild marjoram
Oil, taurine, curcumin, high temperature resistant bacteria preparation, multidimensional and high protein fermentation material, the combinations of these materials can in enteron aisle shape
At enteron aisle protective factors and promote digestion factor, protection, prevention enterogastritis and the immune effect improved are formed to Micropterus salmoides enteron aisle.
Specific embodiment
The present invention will be further described below with reference to examples:
It is a kind of enhance Micropterus salmoides function of intestinal canal health-care feed, forage protein keep 46%, wherein 66 parts of fish meal, 8 parts of starch,
6-7 parts of yeast hydrolyate, 0.5-2 parts of Pulvis Fellis Suis, 0.5-3 parts of high temperature resistant digestive ferment, 1-3 parts of butyrin, origanum oil
0.5-2 parts, 0.5-2 parts of taurine, 0.5-2 parts of curcumin, 1-3 parts of high temperature resistant bacteria preparation, 0.5-1.5 parts of multidimensional and high protein hair
Molten 4 parts and 1 part of meat meal tankage, totally 100 parts of the slurry of 4-8 parts of ferment material, 2 parts of yeast extract, fish.
The health-care feed is particulate material, grain diameter 3.6mm.
A kind of preparation process for the health-care feed enhancing Micropterus salmoides function of intestinal canal, characterized in that preparation step is as follows:
Step 1 mixes complete after accurately weighing the raw material；
Step 2, it is quenched by injection vapor；
Step 3 is fabricated to the model similar with normal diet；
Step 4, air-cooled drying.
A kind of test method for the health-care feed enhancing Micropterus salmoides function of intestinal canal, test selection specification are similar, same
The Micropterus salmoides N tail of batch, is randomly divided into the processing of X group, and Y repetition of every group of processing is each to repeat N/(XY) tail, N X, Y are just
Several times；Wherein, one group is control group, and the Micropterus salmoides of control group is fed using normal diet, and other groups are test group, each to test
The health-care feed that different proportion is respectively adopted in the Micropterus salmoides of group is fed；
60 days experimental periods of test, pre-feeding period 7 days, thoroughly cleaned test cylinder (jar) and carried out pasteurised completely, it is black to test big mouth before testing
Perch is raised in test cylinder (jar), feeds feed by the 3% of weight, and 2 times a day, feeding the time is 09: 00 and 17: 00, and 2 is small after feeding
When fish for residual feed, dry, weighing；
The food ration and dead fish quantity of Micropterus salmoides are recorded during test daily, after the test tail bone venous blood collection.
The forage protein of the normal diet keeps 46%, wherein 66 parts of fish meal, 8 parts of starch, 0.1 part of multidimensional, yeast extract 2
Part, molten 4 parts of the slurry of fish, 1 part of meat meal tankage, 10 parts of dregs of beans, 3 parts of corn flour and 5.9 parts of wheat, totally 100 parts.
Health-care feed specific embodiment is as follows:
Health-care feed proportion: forage protein keeps 46%, wherein 66 parts of fish meal, and 8 parts of starch, 6-7 parts of yeast hydrolyate, Pulvis Fellis Suis
0.5-2 parts, 0.5-3 parts of high temperature resistant digestive ferment, 1-3 parts of butyrin, 0.5-2 parts of origanum oil, 0.5-2 parts of taurine, ginger
0.5-2 parts of flavine, 1-3 parts of high temperature resistant bacteria preparation, 0.5-1.5 parts of multidimensional and 4-8 parts of high protein fermentation material, 2 parts of yeast extract, fish is molten
4 parts, 1 part of meat meal tankage, totally 100 parts of slurry.It is fabricated to the specification of 3.6mm.
Normal diet proportion: forage protein keeps 46%, wherein 66 parts of fish meal, and 8 parts of starch, 0.1 part of multidimensional, yeast extract 2
Part, molten 4 parts, 1 part of meat meal tankage, 10 parts of dregs of beans, 3 parts of corn flour, 5.9 parts of wheat, totally 100 parts of the slurry of fish.It is fabricated to the rule of 3.6mm
Lattice.
Test method one:
Similar in test selection specification, with a batch of 240 tail of Micropterus salmoides, it is randomly divided into 2 groups of processing, 3 weights of every group of processing
It is multiple, each 40 tail of repetition.Control group all uses normal diet, test group health care material.
This 60d(days experimental period of test), pre-feeding period 7d.Test cylinder (jar) (volume is 1000 cubes) is thoroughly cleaned before test,
And pasteurised completely is carried out, test Micropterus salmoides raising feeds feed by the 3% of weight in test cylinder (jar), 2 times a day, feeds the time
For 09: 00 and 17: 00,2 hours (hour) fishes for residual feed after feeding, dries, weighing.It is black that big mouth is recorded during test daily
The food ration of perch and dead fish quantity, after the test tail bone venous blood collection.
Testing index and method:
One, growth performance index stops material 3 days before the formal beginning of test and end, weighs to Micropterus salmoides, the throwing of statistics day
The rate of feeding calculates the production performances index such as average daily gain and feedstuff-meat ratio.
Two, biochemical immunity index takes 4 tail bone venous blood collections for every group after the test, and 4000r/min is centrifuged 15min, point
From serum.Serum IgG, IgM content are measured, in addition each repeating groups are placed in anticoagulant tube after taking 4 fish blood samplings, measure lymphocyte
Quantity and neutrophil leucocyte quantity.
Three, enteron aisle structure detection and flora ratio dissect after test fish blood sampling, take out liver and enteron aisle, be made into slice, see
Structure is examined, and the enteron aisle second half section is cut, accurately weighs 1.0000g, crushing is placed on physiological saline, 1000r/min, centrifugation
10min, takes supernatant, and dilution is placed on culture medium and is separately cultured.
As a result: health-care feed individually feeds Micropterus salmoides, and compared with feeding normal diet, feeding volume is preceding without dominant variation
The gut wall structure integrality of person is higher than the latter, and the integrality of villus is better than the latter, height of naps 15.23%- more than the latter
23.71%, high 12.34%, the IgG of height of naps/Crypt depth odds ratio the latter and IgM are higher than the latter by 16.38%, and 19.63%,
Lymphocyte ratios and granulocyte ratio are higher than the latter by 25.56% and 18.92%.Intestinal beneficial bacterium (Bifidobacterium, lactic acid bacteria,
Enterococcus faecalis etc.) odds ratio the latter is high by 22.15%, and harmful bacteria odds ratio the latter low 11.87%.
Test method two:
1.1 materials and methods
Similar in test selection specification, with a batch of 360 tail of Micropterus salmoides, it is randomly divided into 3 groups of processing, 3 weights of every group of processing
It is multiple, each 40 tail of repetition.Control group all uses normal diet, and the health care material of test group 1 and common material 1:4 mixing feed, test
The health care material of group 2 and common material 1:3 mixing are fed.
This test experimental period 60d, pre-feeding period 7d.Test cylinder (jar) (volume is 1000 cubes) is thoroughly cleaned before test, is gone forward side by side
Row pasteurised completely, test Micropterus salmoides raising feed feed by the 3% of weight in test cylinder (jar), and 2 times a day, feeding the time is 09
: residual feed is fished in 2 hours after feeding in 00 and 17: 00, dries, weighing.The food ration of Micropterus salmoides is recorded during test daily
With dead fish quantity, after the test tail bone venous blood collection.
Testing index and method:
One, stop material 3 days before the formal beginning of test and end, weigh to Micropterus salmoides, statistics feeds rate day, and calculating is average
The production performances index such as daily gain and feedstuff-meat ratio.
Two, biochemical immunity index takes 4 tail bone venous blood collections for every group after the test, and 4000r/min is centrifuged 15min, point
From serum.Serum IgG, IgM content are measured, in addition each repeating groups are placed in anticoagulant tube after taking 4 fish blood samplings, measure lymphocyte
Quantity and neutrophil leucocyte quantity.
Three, enteron aisle structure detection and flora ratio dissect after test fish blood sampling, take out liver and enteron aisle, be made into slice, see
Structure is examined, and the enteron aisle second half section is cut, accurately weighs 1.0000g, crushing is placed on physiological saline, 1000r/min, centrifugation
10min, takes supernatant, and dilution is placed on culture medium and is separately cultured.
As a result: the health care material being mixed with normal diet 1:4 and feeds Micropterus salmoides, compared with only feeding normal diet, is thrown
The amount of feeding has the tendency that rising, and daily gain is higher than the latter by 32.22%, the former gut wall structure integrality is higher than the latter, villus it is complete
Property be better than the latter, for height of naps 21.34% more than the latter, height of naps/Crypt depth odds ratio the latter is high by 15.73%, IgG with
IgM is higher than the latter by 14.34%, and 15.36%, lymphocyte ratios and granulocyte ratio are higher than the latter by 31.28% and 23.34%.
Intestinal beneficial bacterium (Bifidobacterium, lactic acid bacteria, enterococcus faecalis etc.) odds ratio the latter is high by 29.45%, and harmful bacteria odds ratio the latter is low
15.33%。
Health care material is mixed with normal diet 1:4 and 1:3 mixed phase ratio, growth performance, immune performance and enteron aisle defencive function
Without significant difference, for the angle of cost, the former is more excellent.
On the whole, which can effectively maintain intestinal wall integrality, effectively optimize flora ratio, have to enteron aisle certain
Protective effect, furthermore immunity of organism all generally increases, antibacterial ability enhancing.
Claims (10)
1. the forage protein of a kind of health-care feed for enhancing Micropterus salmoides function of intestinal canal, health-care feed keeps 46%, characterized in that
It is calculated according to 100 parts of total raw material, the health-care feed contains 5-7 parts of yeast hydrolyate, 0.5-2 parts of Pulvis Fellis Suis, high temperature resistant digestive ferment
0.5-3 parts, 1-3 parts of butyrin, 0.5-2 parts of origanum oil, 0.5-2 parts of taurine, 0.5-2 parts of curcumin, high temperature resistant bacterium
1-3 parts of preparation, 0.5-1.5 parts of multidimensional and 4-8 parts of high protein fermentation material.
2. enhancing the health-care feed of Micropterus salmoides function of intestinal canal according to claim 1, characterized in that the health-care feed is also
Contain fish meal, starch, yeast extract, the molten slurry of fish and meat meal tankage.
3. enhancing the health-care feed of Micropterus salmoides function of intestinal canal according to claim 2, characterized in that in the health-care feed
66 parts of fish meal, 8 parts of starch, 5-7 parts of yeast hydrolyate, 0.5-2 parts of Pulvis Fellis Suis, 0.5-3 parts of high temperature resistant digestive ferment, three butyric acid glycerol
1-3 parts of rouge, 0.5-2 parts of origanum oil, 0.5-2 parts of taurine, 0.5-2 parts of curcumin, 1-3 parts of high temperature resistant bacteria preparation, multidimensional 0.5-
1.5 parts, 4-8 parts of high protein fermentation material, 2 parts of yeast extract, molten 4 parts and 1 part of meat meal tankage of the slurry of fish, add up to totally 100 parts.
4. enhancing the health-care feed of Micropterus salmoides function of intestinal canal according to claim 3, characterized in that in the health-care feed
66 parts of fish meal, 8 parts of starch, 5 parts of yeast hydrolyate, 1 part of Pulvis Fellis Suis, 1 part of high temperature resistant digestive ferment, 2 parts of butyrin, wild marjoram
Oily 1 part, 1 part of taurine, 1 part of curcumin, 1.5 parts of high temperature resistant bacteria preparation, 0.5 part of multidimensional, 5 parts of high protein fermentation material, yeast extract 2
Part, molten 4 parts and 1 part of meat meal tankage, totally 100 parts of the slurry of fish.
5. enhancing the health-care feed of Micropterus salmoides function of intestinal canal according to claim 1, characterized in that the health-care feed is
Particulate material, grain diameter 1.5-8.0mm.
6. a kind of preparation process for the health-care feed for enhancing Micropterus salmoides function of intestinal canal according to claim 4, characterized in that
Preparation step is as follows:
Step 1 mixes complete after accurately weighing the raw material；
Step 2, it is quenched by injection vapor；
Step 3 is fabricated to the model similar with normal diet；
Step 4, air-cooled drying.
7. a kind of test method for the health-care feed for enhancing Micropterus salmoides function of intestinal canal according to claim 4, characterized in that
Test selects similar in specification, with a batch of Micropterus salmoides N tail, is randomly divided into X group and handles, Y repetition of every group of processing, each
Repeat N/(XY) tail, the positive several times of N X, Y；Wherein, one group is control group, and the Micropterus salmoides of control group is fed using normal diet
It supports, other groups are test group, and the health-care feed that different proportion is respectively adopted in the Micropterus salmoides of each test group is fed；
60 days experimental periods of test, pre-feeding period 7 days, thoroughly cleaned test cylinder (jar) and carried out pasteurised completely, it is black to test big mouth before testing
Perch is raised in test cylinder (jar), feeds feed by the 3% of weight, and 2 times a day, feeding the time is 09: 00 and 17: 00, and 2 is small after feeding
When fish for residual feed, dry, weighing；
The food ration and dead fish quantity of Micropterus salmoides are recorded during test daily, after the test tail bone venous blood collection.
8. enhancing the test method of the health-care feed of Micropterus salmoides function of intestinal canal according to claim 7, characterized in that described
N is 240, X 2, and Y 3, test group is one group, and the ratio of health-care feed is 100%.
9. enhancing the test method of the health-care feed of Micropterus salmoides function of intestinal canal according to claim 7, characterized in that described
N is 360, X 3, and Y 3, test group is two groups, and two groups of test groups are respectively the first test group and the second test group, the first test
The ratio of health-care feed and normal diet is 1:4 in group, and the ratio of health-care feed and normal diet is 1:3 in the second test group.
10. enhancing the test method of the health-care feed of Micropterus salmoides function of intestinal canal according to claim 7, characterized in that institute
The forage protein for stating normal diet keeps 46%, wherein the molten slurry 4 of 66 parts of fish meal, 8 parts of starch, 0.1 part of multidimensional, 2 parts of yeast extract, fish
Part, 1 part of meat meal tankage, 10 parts of dregs of beans, 3 parts of corn flour and 5.9 parts of wheat, totally 100 parts.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910587902.2A CN110301549A (en) | 2019-07-02 | 2019-07-02 | A kind of health-care feed and preparation method thereof enhancing Micropterus salmoides function of intestinal canal and test method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910587902.2A CN110301549A (en) | 2019-07-02 | 2019-07-02 | A kind of health-care feed and preparation method thereof enhancing Micropterus salmoides function of intestinal canal and test method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110301549A true CN110301549A (en) | 2019-10-08 |
Family
ID=68078722
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910587902.2A Pending CN110301549A (en) | 2019-07-02 | 2019-07-02 | A kind of health-care feed and preparation method thereof enhancing Micropterus salmoides function of intestinal canal and test method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110301549A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101491296A (en) * | 2008-01-23 | 2009-07-29 | 北京因科瑞斯医药科技有限公司 | Natural feed additive and preparation method thereof |
| CN104664167A (en) * | 2015-03-13 | 2015-06-03 | 广东雅琪生物科技有限公司 | Micropterus salmoides mixed feed with yeast hydrolysate replacing fish meal |
| CN105285344A (en) * | 2015-12-04 | 2016-02-03 | 广东双湖饲料有限公司 | Micropterus salmonides compound feed and preparation method thereof |
| CN108783109A (en) * | 2018-07-03 | 2018-11-13 | 珠海容川饲料有限公司 | Improve the mixed feed and its preparation method and application of Micropterus salmoides intestinal health |
| CN109198265A (en) * | 2018-09-26 | 2019-01-15 | 佛山市顺德区旺海饲料实业有限公司 | A kind of megathermal period food calling promotees the largemouth bass expanded pellet diet and preparation method thereof of digestion |
| CN109845918A (en) * | 2019-01-08 | 2019-06-07 | 珠海市德海生物科技有限公司 | Low meal compound feed of a kind of largemouth bass and preparation method thereof |
-
2019
- 2019-07-02 CN CN201910587902.2A patent/CN110301549A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101491296A (en) * | 2008-01-23 | 2009-07-29 | 北京因科瑞斯医药科技有限公司 | Natural feed additive and preparation method thereof |
| CN104664167A (en) * | 2015-03-13 | 2015-06-03 | 广东雅琪生物科技有限公司 | Micropterus salmoides mixed feed with yeast hydrolysate replacing fish meal |
| CN105285344A (en) * | 2015-12-04 | 2016-02-03 | 广东双湖饲料有限公司 | Micropterus salmonides compound feed and preparation method thereof |
| CN108783109A (en) * | 2018-07-03 | 2018-11-13 | 珠海容川饲料有限公司 | Improve the mixed feed and its preparation method and application of Micropterus salmoides intestinal health |
| CN109198265A (en) * | 2018-09-26 | 2019-01-15 | 佛山市顺德区旺海饲料实业有限公司 | A kind of megathermal period food calling promotees the largemouth bass expanded pellet diet and preparation method thereof of digestion |
| CN109845918A (en) * | 2019-01-08 | 2019-06-07 | 珠海市德海生物科技有限公司 | Low meal compound feed of a kind of largemouth bass and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 陈晓春: "《饲料加工工艺与设备》", 31 August 2018 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102041238B (en) | Lactobacillus plantarum, method for fermenting and preparing bacteriocin of Lactobacillus plantarum, and application of Lactobacillus plantarum and bacteriocin | |
| Setyaningrum et al. | The effect of synbiotic (inulin extracted from gembili tuber and Lactobacillus plantarum) on growth performance, intestinal ecology and haematological indices of broiler chicken | |
| KR102255611B1 (en) | Method for preparing fermented total mixed ration using microbial strain complex | |
| CN102669427B (en) | Paecilomyces militaris Liang sp.nov. fermented feed additive as well as preparation method and application thereof | |
| CN103749981A (en) | Application of protocatechuic acid as feed additive in livestock breeding | |
| CN113142385A (en) | Formula and preparation method of bone-growing meat nutritional cat food | |
| CN102078522A (en) | Chinese medicine composition for treating enterovirus syndrome in chicken | |
| JP2015221001A (en) | In-vivo lipid deposition inhibitor, feed and rearing method | |
| CN111528368A (en) | Preparation method and application of eucommia ulmoides fermentation product | |
| Adegbesan et al. | Growth performance, haematology and histopathology of African catfish (Clarias gariepinus) fed varying levels of Aloe barbadensis leaves | |
| KR100672657B1 (en) | Assorted feedcontained fibroid material and its preparation method | |
| Yu et al. | Dietary chlorella (Chlorella vulgaris) supplementation effectively improves body color, alleviates muscle inflammation and inhibits apoptosis in largemouth bass (Micropterus salmoides) | |
| Chimsung et al. | Fermented golden apple snails as an alternative protein source in sex-reversed red tilapia (Oreochromis niloticus x O. mossambicus) diets | |
| CN111513210A (en) | Aquaculture feed | |
| CN105076707B (en) | A kind of ox fattening feed based on jujube | |
| Sugiharto et al. | Hematological and intestinal responses of broilers to dietary supplementations of lactic fermented turmeric, black pepper or a mixture of both. | |
| CN110301549A (en) | A kind of health-care feed and preparation method thereof enhancing Micropterus salmoides function of intestinal canal and test method | |
| CN107080045A (en) | A kind of healthy pig feed of the cultivation containing chlorella and preparation method | |
| WO2022154010A1 (en) | Composition for controlling intestinal bacterial flora | |
| CN108967747A (en) | A kind of compound feed for snakeheads and preparation method thereof | |
| CN109385406A (en) | One plant of vibrio parahaemolyticus phage and its application in terms of enhancing aquatic livestock immunity | |
| CN109221628A (en) | A kind of compound feed for snakeheads and preparation method thereof | |
| CN116746634A (en) | A health pig feed containing spirulina composition and its preparation method | |
| Nurhayati et al. | The potency of curing fish waste pellet for growth and protein level of African sharptooth catfish (Clarias gariepinus) | |
| CN107712274A (en) | It is a kind of using sugarcane caudal lobe as biological feedstuff of major ingredient and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |