CN110301455A - Application of the volatile materials that grandson Ah streptomycete generates in control of plant disease - Google Patents
Application of the volatile materials that grandson Ah streptomycete generates in control of plant disease Download PDFInfo
- Publication number
- CN110301455A CN110301455A CN201910604694.2A CN201910604694A CN110301455A CN 110301455 A CN110301455 A CN 110301455A CN 201910604694 A CN201910604694 A CN 201910604694A CN 110301455 A CN110301455 A CN 110301455A
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- China
- Prior art keywords
- grandson
- streptomycete
- culture
- volatile materials
- wheat
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241001655322 Streptomycetales Species 0.000 title claims abstract description 56
- 239000000463 material Substances 0.000 title claims abstract description 51
- 201000010099 disease Diseases 0.000 title claims abstract description 28
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 28
- 241001443921 Phytophthora litchii Species 0.000 claims abstract description 22
- 230000002401 inhibitory effect Effects 0.000 claims abstract description 8
- 241000209140 Triticum Species 0.000 claims description 51
- 235000021307 Triticum Nutrition 0.000 claims description 51
- 235000021028 berry Nutrition 0.000 claims description 37
- 239000001963 growth medium Substances 0.000 claims description 18
- 241000196324 Embryophyta Species 0.000 claims description 16
- 241000894006 Bacteria Species 0.000 claims description 14
- 238000002360 preparation method Methods 0.000 claims description 10
- 239000000725 suspension Substances 0.000 claims description 7
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 101000610620 Homo sapiens Putative serine protease 29 Proteins 0.000 claims description 5
- 102100040345 Putative serine protease 29 Human genes 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 244000205754 Colocasia esculenta Species 0.000 claims description 4
- 235000006481 Colocasia esculenta Nutrition 0.000 claims description 4
- 241000233614 Phytophthora Species 0.000 claims description 4
- 241000233616 Phytophthora capsici Species 0.000 claims description 4
- 208000025865 Ulcer Diseases 0.000 claims description 4
- DCAYPVUWAIABOU-UHFFFAOYSA-N hexadecane Chemical compound CCCCCCCCCCCCCCCC DCAYPVUWAIABOU-UHFFFAOYSA-N 0.000 claims description 4
- YCOZIPAWZNQLMR-UHFFFAOYSA-N pentadecane Chemical compound CCCCCCCCCCCCCCC YCOZIPAWZNQLMR-UHFFFAOYSA-N 0.000 claims description 4
- 238000007790 scraping Methods 0.000 claims description 4
- BGHCVCJVXZWKCC-UHFFFAOYSA-N tetradecane Chemical compound CCCCCCCCCCCCCC BGHCVCJVXZWKCC-UHFFFAOYSA-N 0.000 claims description 4
- IIYFAKIEWZDVMP-UHFFFAOYSA-N tridecane Chemical compound CCCCCCCCCCCCC IIYFAKIEWZDVMP-UHFFFAOYSA-N 0.000 claims description 4
- 231100000397 ulcer Toxicity 0.000 claims description 4
- 241000223600 Alternaria Species 0.000 claims description 3
- 240000006432 Carica papaya Species 0.000 claims description 3
- 235000009467 Carica papaya Nutrition 0.000 claims description 3
- 241000223195 Fusarium graminearum Species 0.000 claims description 3
- 241001330975 Magnaporthe oryzae Species 0.000 claims description 3
- 241001622896 Pythium myriotylum Species 0.000 claims description 3
- 241000187181 Streptomyces scabiei Species 0.000 claims description 3
- 235000009754 Vitis X bourquina Nutrition 0.000 claims description 3
- 235000012333 Vitis X labruscana Nutrition 0.000 claims description 3
- 240000006365 Vitis vinifera Species 0.000 claims description 3
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 3
- 235000013339 cereals Nutrition 0.000 claims description 3
- -1 dimethyl lignocerane Chemical compound 0.000 claims description 3
- 150000002148 esters Chemical class 0.000 claims description 3
- 238000002470 solid-phase micro-extraction Methods 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- DTGKSKDOIYIVQL-WEDXCCLWSA-N (+)-borneol Chemical compound C1C[C@@]2(C)[C@@H](O)C[C@@H]1C2(C)C DTGKSKDOIYIVQL-WEDXCCLWSA-N 0.000 claims description 2
- REPVLJRCJUVQFA-UHFFFAOYSA-N (-)-isopinocampheol Natural products C1C(O)C(C)C2C(C)(C)C1C2 REPVLJRCJUVQFA-UHFFFAOYSA-N 0.000 claims description 2
- HFOFYNMWYRXIBP-MOPGFXCFSA-N 2-methyl-7S,8R-Epoxy-octadecane Chemical compound CCCCCCCCCC[C@H]1O[C@H]1CCCCC(C)C HFOFYNMWYRXIBP-MOPGFXCFSA-N 0.000 claims description 2
- GOLCXWYRSKYTSP-UHFFFAOYSA-N Arsenious Acid Chemical compound O1[As]2O[As]1O2 GOLCXWYRSKYTSP-UHFFFAOYSA-N 0.000 claims description 2
- PDSNLYSELAIEBU-UHFFFAOYSA-N Longifolene Chemical compound C1CCC(C)(C)C2C3CCC2C1(C)C3=C PDSNLYSELAIEBU-UHFFFAOYSA-N 0.000 claims description 2
- ZPUKHRHPJKNORC-UHFFFAOYSA-N Longifolene Natural products CC1(C)CCCC2(C)C3CCC1(C3)C2=C ZPUKHRHPJKNORC-UHFFFAOYSA-N 0.000 claims description 2
- 229940116229 borneol Drugs 0.000 claims description 2
- CKDOCTFBFTVPSN-UHFFFAOYSA-N borneol Natural products C1CC2(C)C(C)CC1C2(C)C CKDOCTFBFTVPSN-UHFFFAOYSA-N 0.000 claims description 2
- DTGKSKDOIYIVQL-UHFFFAOYSA-N dl-isoborneol Natural products C1CC2(C)C(O)CC1C2(C)C DTGKSKDOIYIVQL-UHFFFAOYSA-N 0.000 claims description 2
- 238000000605 extraction Methods 0.000 claims description 2
- HMMGMWAXVFQUOA-UHFFFAOYSA-N octamethylcyclotetrasiloxane Chemical compound C[Si]1(C)O[Si](C)(C)O[Si](C)(C)O[Si](C)(C)O1 HMMGMWAXVFQUOA-UHFFFAOYSA-N 0.000 claims description 2
- 239000000758 substrate Substances 0.000 claims description 2
- RZJRJXONCZWCBN-UHFFFAOYSA-N octadecane Chemical compound CCCCCCCCCCCCCCCCCC RZJRJXONCZWCBN-UHFFFAOYSA-N 0.000 claims 2
- DCERHCFNWRGHLK-UHFFFAOYSA-N C[Si](C)C Chemical compound C[Si](C)C DCERHCFNWRGHLK-UHFFFAOYSA-N 0.000 claims 1
- 229940038384 octadecane Drugs 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 abstract description 5
- 241000233866 Fungi Species 0.000 abstract description 4
- 239000003223 protective agent Substances 0.000 abstract description 2
- 238000012545 processing Methods 0.000 description 19
- 238000002474 experimental method Methods 0.000 description 7
- 230000003385 bacteriostatic effect Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 244000052769 pathogen Species 0.000 description 6
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 238000011081 inoculation Methods 0.000 description 5
- 230000001717 pathogenic effect Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 3
- 244000183278 Nephelium litchi Species 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 210000002421 cell wall Anatomy 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 230000018044 dehydration Effects 0.000 description 3
- 238000006297 dehydration reaction Methods 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000012549 training Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- UUGLJVMIFJNVFH-UHFFFAOYSA-N Hexyl benzoate Chemical compound CCCCCCOC(=O)C1=CC=CC=C1 UUGLJVMIFJNVFH-UHFFFAOYSA-N 0.000 description 2
- 240000008790 Musa x paradisiaca Species 0.000 description 2
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000000845 anti-microbial effect Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 230000009036 growth inhibition Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- MLFHJEHSLIIPHL-UHFFFAOYSA-N isoamyl acetate Chemical compound CC(C)CCOC(C)=O MLFHJEHSLIIPHL-UHFFFAOYSA-N 0.000 description 2
- 230000003902 lesion Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- ZYURHZPYMFLWSH-UHFFFAOYSA-N octacosane Chemical compound CCCCCCCCCCCCCCCCCCCCCCCCCCCC ZYURHZPYMFLWSH-UHFFFAOYSA-N 0.000 description 2
- 230000003449 preventive effect Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 241000186046 Actinomyces Species 0.000 description 1
- 244000153158 Ammi visnaga Species 0.000 description 1
- 235000010585 Ammi visnaga Nutrition 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 206010003694 Atrophy Diseases 0.000 description 1
- 241000193738 Bacillus anthracis Species 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 239000004593 Epoxy Substances 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 241001645777 Muscodor albus Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000228150 Penicillium chrysogenum Species 0.000 description 1
- 241000122123 Penicillium italicum Species 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 241000221696 Sclerotinia sclerotiorum Species 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 241000393054 Streptomyces abikoensis Species 0.000 description 1
- 241000223261 Trichoderma viride Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- SFCVEUVVOJFYSX-UHFFFAOYSA-J [Pb+2].C(CC(O)(C(=O)[O-])CC(=O)[O-])(=O)[O-].C(C)(=O)[O-].[U+6] Chemical compound [Pb+2].C(CC(O)(C(=O)[O-])CC(=O)[O-])(=O)[O-].C(C)(=O)[O-].[U+6] SFCVEUVVOJFYSX-UHFFFAOYSA-J 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000443 biocontrol Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 1
- 230000002554 disease preventive effect Effects 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 238000003898 horticulture Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229940117955 isoamyl acetate Drugs 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 229910001092 metal group alloy Inorganic materials 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 1
- FHMDYDAXYDRBGZ-UHFFFAOYSA-N platinum tin Chemical compound [Sn].[Pt] FHMDYDAXYDRBGZ-UHFFFAOYSA-N 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000007665 sagging Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 230000004763 spore germination Effects 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 210000004895 subcellular structure Anatomy 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 238000000352 supercritical drying Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 210000003934 vacuole Anatomy 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- GRJUENNHVNYCHD-UHFFFAOYSA-N ξ-3-methyldodecane Chemical compound CCCCCCCCCC(C)CC GRJUENNHVNYCHD-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/18—Vapour or smoke emitting compositions with delayed or sustained release
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/06—Oxygen or sulfur directly attached to a cycloaliphatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/20—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom three- or four-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N55/00—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N55/00—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur
- A01N55/02—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur containing metal atoms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P9/00—Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses application of the volatile materials in control of plant disease that grandson Ah streptomycete generates, the volatile materials that specific open grandson Ah streptomycete generates is able to suppress the growth of a variety of cause of disease fungus such as Peronophythora Litchii, and inhibiting rate is up to 63~100%.The present invention has also further clarified 14 kinds of compounds with obvious bacteriostasis in the volatile materials of grandson Ah streptomycete generation, provides thinking to study the protective agents of the phytopathogens such as Peronophythora Litchii from now on.
Description
Technical field
The volatile materials generated the present invention relates to biocontrol of plant disease field more particularly to grandson Ah streptomycete is being planted
Application in object disease control.
Background technique
Volatile materials is the compound of a kind of low molecular weight (300Da), and nonpolarity, volatile, vapour pressure is higher, room temperature
Under can achieve 0.01kPa (Morath et al., 2012).It is just reported early in Moore-Landecker&Stotzky in 1973
The volatile materials that actinomyces generate causes the bacterium solutions such as Aspergillus conidia atrophy and Penicillium notatum, sickle-like bacteria, Trichoderma viride to be steeped
Increase.(2001) people such as Strobel reports that the volatile materials that endogenetic fungus Muscodor albus is generated can by fumigating system
Prevent and treat a variety of postharvest diseases (Mercier, 2004；Mercier et al., 2005) and soil-borne disease.In recent years, more and more
Researcher be engaged in the research of microorganism volatile materials.Wan Mingguo (2008) reports Pood's zipper in closed container
Mould can effectively inhibit the generation of grey mould fruit rot of strawberry, rice sheath blight disease, sclerotinia sclerotiorum.What styreptomyces globispotus strain JK-1 was generated waves
Volatile material causes ash arrhizus bacteria, Citrus fungal hyphae deformity, cell membrane to be destroyed, and inhibits mycelia growth, spore germination
And illumination, and the generation (Li Qili, 2011) of Penicillium italicum, Zhang Qinghua (2014) report can be effectively controlled
CanR-46 is the endogenetic fungus of rape, and to generate volatile substance can inhibit the growth of sclerotinite.
As it can be seen that research of the volatile materials of microorganism generation in terms of pathogen prevention and treatment has been achieved with certain achievement.
But relatively fewer for the research of grandson's Ah streptomycete volatile materials at present, grandson Ah streptomycete is still not clear in plant in researcher
Important function in terms of disease control needs to further investigate.
Summary of the invention
The present invention provides a kind of application of the volatile materials of grandson Ah streptomycete generation in control of plant disease, effectively fills out
Mend technological gap of the grandson Ah streptomycete in terms of control of plant disease.
The technical solution adopted by the present invention is as follows:
It is described the present invention provides a kind of application of the volatile materials of grandson Ah streptomycete generation in control of plant disease
Plant disease is by Peronophythora Litchii, Phytophthora capsici, taro phytophthora, P. myriotylum, Alternaria, fusarium graminearum, banana charcoal
Plant disease caused by subcutaneous ulcer germ, rice blast fungus, grape ulcer bacterium or papaya Streptomyces scabies.The volatile materials
There is apparent inhibiting effect for the growth of Peronophythora Litchii mycelia, sporangium yield and egg spore yield.
The present invention is also further analyzed by GC-MS, specifies that the ingredient of volatile materials includes: three (front three of arsenious acid
Base silicon substrate) ester, the different borneol of 2- methyl, octamethylcy-clotetrasiloxane, tridecane, dimethyl lignocerane, hexyl-benzoate, 2,6,
10- methyl dodecane, the tetradecane, cis- -7,8- epoxy -2- methyl octadecane, 2,6,10- methyltridec, positive octacosane,
N-pentadecane, hexadecane and longifolene.
The preparation method of the volatile materials, comprising the following steps:
S1: grandson's Ah streptomycete bacterial strain is crossed on PDA plate culture 7d, and scraping Fresh spores access ISP2 culture medium
Afterwards, it is placed in shaken cultivation on shaking table, then prepares spore suspension, is inoculated on wheat berry culture medium and cultivates, obtains A Sunlian
Mould wheat culture；
S2: wheat culture is put into bottle, after sealing is placed, extraction.
Further, step S1 are as follows: cross grandson's Ah streptomycete bacterial strain culture 7d on PDA plate, scrapes fresh spore
In triangular flask of the son access containing 100mL ISP2 culture medium, it is subsequently placed on shaking table, with 200rpm, 28 DEG C of shaken cultivation 3d,
Prepare concentration 1 × 107The spore suspension of a spore/mL, is inoculated on wheat berry culture medium in the ratio of 1mL/100g, is shaken up
Afterwards, 20d is cultivated under the conditions of being placed in 25 DEG C, every 3d shakes up wheat culture medium once therebetween.
Step S2 are as follows: the grandson's Ah streptomycete wheat culture for weighing culture 20d is put into bottle, is placed at 25 DEG C after sealing
Then 12h is extracted with SPME extracting head.
Compared with prior art, the beneficial effects of the present invention are:
(1) volatile materials that grandson Ah streptomycete generates is able to suppress the growth of a variety of cause of disease fungus such as Peronophythora Litchii,
Inhibiting rate is up to 63~100%.
(2) 14 kinds of compounds with obvious bacteriostasis in the volatile materials of grandson Ah streptomycete generation are specified, are
The protective agents for studying Peronophythora Litchii from now on provide direction.
(3) specify the volatile materials of grandson Ah streptomycete generation for the growth of Peronophythora Litchii mycelia, sporangium yield
There is apparent inhibiting effect with egg spore yield.
Detailed description of the invention
Fig. 1 is that different incubation times generate volatile materials bacteriostatic activity impact effect figure to grandson Ah streptomycete TJGA-19；
A in figure, b, c, d are respectively to compare the culture for cultivating 10d, 20d, 30d on wheat broth with grandson Ah streptomycete TJGA-19
Processing；
Fig. 2 is that different incubation times generate volatile materials bacteriostatic activity influence result system to grandson Ah streptomycete TJGA-19
Meter figure；
Fig. 3 is the volatile materials of different amounts of grandson Ah streptomycete TJGA-19 wheat berry culture generation to lichee frost epidemic disease
The impact effect figure of mould silk growth；In figure, a, b, c, d, e, f, g, h, i is respectively to compare and 2g/L, 4g/L, 8g/L, 12g/
L, grandson's Ah streptomycete TJGA-19 wheat berry culture processing of 16g/L, 24g/L, 32g/L, 40g/L；
Fig. 4 is the volatile materials of different amounts of grandson Ah streptomycete TJGA-19 wheat berry culture generation to lichee frost epidemic disease
The influence result statistical chart of mould silk growth；
Fig. 5 is the volatile materials of different amounts of grandson Ah streptomycete TJGA-19 wheat berry culture generation to lichee frost epidemic disease
The influence result statistical chart of mould sporangium yield；
Fig. 6 is that the volatile materials that different amounts of grandson Ah streptomycete TJGA-19 wheat berry culture generates produces egg spore
The impact effect figure of amount；A, b, c, d, e, f, g, h, i, j, k be respectively compare and 3g/L, 4g/L, 6g/L, 8g/L, 12g/L,
Grandson's Ah streptomycete TJGA-19 wheat berry culture of 16g/L, 24g/L, 32g/L, 40g/L are handled；
Fig. 7 is that the volatile materials that different amounts of grandson Ah streptomycete TJGA-19 wheat berry culture generates produces egg spore
The influence result statistical chart of amount；
Fig. 8 is impact effect figure of the scanning electron microscopic observation volatile materials to Peronophythora Litchii form；A, b, c are control；
Grandson's Ah streptomycete TJGA-19 wheat berry culture that d, e, f are 100g/L is handled.
Fig. 9 is impact effect figure of the transmission electron microscope observing volatile materials to Peronophythora Litchii mycelia subcellular structure；A,
B, c are control；Grandson's Ah streptomycete TJGA-19 wheat berry culture that d, e, f are 100g/L is handled.A, d are that sporangium is longitudinally cut
Face；B, e are mycelia transverse direction section；C, f are mycelia longitudinal direction section；N: nucleus；W: cell wall；
Figure 10 is preventive effect effect picture of the volatile materials to excised leaf lichee frost epidemic disease；A, b, c, d, e are respectively to compare
It is handled with grandson's Ah streptomycete TJGA-19 wheat berry culture of 8g/L, 16g/L, 24g/L, 32g/L；
Figure 11 is preventive effect result statistical chart of the volatile materials to excised leaf lichee frost epidemic disease.
Specific embodiment
Below by specific embodiment combination attached drawing, invention is further described in detail.
Experimental method used in the embodiment of the present invention is conventional method unless otherwise specified.
Material used in the embodiment of the present invention, reagent etc., are commercially available unless otherwise specified.
The Litchi Varieties that experiment inoculation of embodiment of the present invention Litchi Leaves used are chosen are bosom branch.
Litchi Leaves used in the embodiment of the present invention pick up from College of Horticulture, Agricultural University Of South China Experimental Base lichee orchard, choosing
Take tender children, shape, size, leaf age consistent, no disease and pests harm, the blade having no mechanical damage are rinsed well before inoculation with flowing water, room temperature
It air-dries.
Grandson Ah streptomycete TJGA-19 (Streptomyces abikoensis TJGA-19) of the present invention, it is big by Hainan
It learns the separation of plant protection institute plant disease biology prevention and control laboratory to save, can be obtained by conventional method in the prior art.
The pathogens such as Peronophythora Litchii described in the embodiment of the present invention 8, Phytophthora capsici, taro phytophthora are University Of Hainan's plant protection
The separation of institute's plant disease biology prevention and control laboratory saves, and can be obtained by conventional method in the prior art.
Wheat berry culture medium described in the embodiment of the present invention: wheat being filled up after boiling is boiled to skin crack and is filtered, is dried, point
Dress sterilizing.
Embodiment 1, grandson Ah streptomycete TJGA-19 generate the preparation of volatile materials
Bacterial strain TJGA-19 is crossed on PDA plate and is cultivated 7 days, scraping Fresh spores access is trained containing 100mL ISP2
Support base 250mL triangular flask in, be subsequently placed in 28 DEG C of shaken cultivation 3d on the shaking table of 200rpm, prepare spore suspension (about 1 ×
107A spore/mL), it is inoculated into the ratio inoculum concentration of 1mL/100g (V/W) on sterile wheat berry culture medium.After shaking up, set
20d is cultivated under the conditions of 25 DEG C, every 3d shakes up wheat culture medium once therebetween, so as to bacterial strain TJGA-19 homoepitaxial.
Embodiment 2, incubation time generate the influence of volatile materials bacteriostatic activity to grandson Ah streptomycete TJGA-19
In big culture dish (H=30mm, total inner volume 500mL) place four small culture dish bottom Wherein 3 PDA culture mediums of the small culture dish containing about 5mL, and one piece of Peronophythora Litchii of centre inoculation in the medium
Bacteria cakeAnother small culture dish places 40g/L grandson Ah streptomycete TJGA-19 wheat berry culture (25 DEG C of conditions
It is lower to have cultivated 10d, 20d, 30d respectively), it is control with the sterile wheat berry of 40g/L, then seals big culture dish.At 25 DEG C
Constant temperature incubation 6d measures colony diameter and calculates bacteriostasis rate.3 repetitions of each processing, experiment are repeated 3 times.The result is shown in Figure 1 and figure
2。
Mycelial growth inhibition rate (%)=(control colony diameter-processing colony diameter)/control colony diameter × 100
The result shows that:
Incubation time is different, and there are biggish differences for the volatile materials bacteriostatic activity that grandson Ah streptomycete TJGA-19 is generated.
The volatile materials bacteriostatic activity that grandson Ah streptomycete TJGA-19 cultivates 20d generation on wheat berry culture medium is most strong, and bacteriostasis rate reaches
74.1%, and the bacteriostasis rate for cultivating the processing of 10d and 30d is respectively 54.9% and 63.7%, substantially less than culture 20d is handled
Bacteriostasis rate.
The collection and identification of embodiment 3, volatile materials
The grandson's Ah streptomycete TJGA-19 wheat berry culture 4g for weighing culture 20d is put into 20mL bottle, close with tin platinum paper
It is honored as a queen at 25 DEG C and places 12h, then entered in bottle with SPME extracting head skewer, release fiber head [metal alloy (100 μ of PDMS
M) fiber head is withdrawn after] adsorbing 30min, by extracting head hand sampling to GC-MS (Agilent 7890B American), 250
Desorption 3min at DEG C carries out GC-MS analysis.GC-MS operating condition is referring to (Strobel, et al.2001).Computer is automatic
By obtained gas componant mass spectrum and international standard database (NIST 14Mass Spectrometry Library
Databases) data are compared, and identify the ingredient of waving property substance.With sterile blank equivalent wheat be control, will control with
Simultaneous substance removes in grandson's Ah streptomycete TJGA-19 wheat berry culture.3 repetitions of each processing.
The volatile materials that the grandson Ah streptomycete TJGA-19 for detecting culture 20d through GC-MS is generated, obtains 14 kinds of compounds
(table 1).These compound majorities belong to alkenes, esters, organic acid, alkane etc..
Table 1
Embodiment 4, volatile materials are grown to Peronophythora Litchii mycelia and the influence of sporangium yield
By 4 small culture dish bottomIt is placed on a big culture dishIn, wherein 3 small trainings
PDA culture medium of the ware containing about 5mL is supported, in the medium one piece of Peronophythora Litchii bacteria cake of centre inoculationAnother is small
Culture dish places grandson's Ah streptomycete TJGA-19 wheat berry culture of embodiment 1, seals big culture dish, 25 DEG C of perseverances with sealed membrane
Temperature culture 6d, measures colony diameter, then aseptic water washing bacterium colony surface, filters off mycelia, count sporangium yield.Experimental setup
8 processing, the amount of grandson's Ah streptomycete TJGA-19 wheat berry culture be respectively 2g/L, 4g/L, 8g/L, 12g/L, 16g/L,
24g/L, 32g/L, 40g/L are control with the sterile blank wheat berry of equivalent.3 repetitions of each processing, experiment are repeated 3 times.As a result
See Fig. 3-Fig. 6.
The results showed that the amount of wheat berry culture is bigger, bacteriostatic activity is stronger.6d is cultivated at 25 DEG C, control
Colony diameter 47.3mm, when the amount of culture is 2g/L, 4g/L, 8g/L, colony diameter be respectively 46.8mm and 46.2mm and
45.4mm, there was no significant difference compared with the control for they, when the amount of culture is 12g/L, 16g/L, 24g/L, 32g/L, 40g/L
When, colony diameter is respectively 39.6mm, 33.2mm, 26.3mm, 20.6mm, 1.22mm, the colony diameter substantially less than compareed.
6d is cultivated at 25 DEG C, the sporangium yield of control is 129.2 × 104A sporangium/ware, the amount of culture are
When 2g/L, 4g/L, sporangium yield is 124.7 × 104、123.5×104A sporangium/ware, it is poor without conspicuousness compared with the control
It is different, culture amount be 8g/L, 12g/L, 16g/L, 24g/L processing in, sporangium yield is respectively 78 × 104、51.3
×104、5.3×104With 2.6 × 104A sporangium/ware, culture amount be 32g/L, 40g/L processing in, without spore
Capsule is formed.
Influence of 5 volatile materials of embodiment to Peronophythora Litchii egg spore yield
By 4 small culture dish bottomIt is placed on a big culture dishIn, wherein 3 small trainings
CA culture medium of the ware containing about 3mL is supported, places one piece of Peronophythora Litchii bacteria cake in plate centerAnother small training
Grandson's Ah streptomycete TJGA-19 wheat berry culture that ware places embodiment 1 is supported, seals big culture dish, 25 DEG C of constant temperature with sealed membrane
Dark culturing 14d cuts three ferfas silk block at random in the region from vaccination 10mmIt is placed in 10mL centrifugation
3mL ultrapure water is added in Guan Zhong, is homogenized 2min with high-speed homogenization machine (6000rpm), then counts 50 μ L egg spores under the microscope
Quantity, to acquire the egg spore quantity (Flier et al., 2001) of unit area.Experimental setup 10 processing, A Sunlian
The amount of mould TJGA-19 wheat berry culture is respectively 3g/L, 4g/L, 6g/L, 8g/L, 12g/L, 16g/L, 24g/L, 32g/L
And 40g/L, it is control with the sterile blank wheat berry of equivalent.3 repetitions of each processing, experiment are repeated 3 times.
Egg spore yield (a/cm of unit area2Egg spore quantity × 60/2.36 in the μ L suspension of)=50
The results showed that cultivating 14d under 25 DEG C of dark conditions, generated largely in the processing group of control group and 3g/L
Egg spore, respectively 2686.4/cm2, 2669.5/cm2；When the amount of culture is 4g/L, egg spore yield is
2347.4/cm2, it is remarkably decreased compared with the control；When the amount of culture is 6g/L, 8g/L, egg spore yield sharply declines
To 1466.1/cm2, 57.3/cm2；When the amount of culture increases to 12g/L, 16g/L, 24g/L, 32g/L, 40g/L, do not have
There is egg spore to form (Fig. 7).
Influence of 6 volatile materials of embodiment to Peronophythora Litchii ultra microstructure
First Peronophythora Litchii is seeded on CA culture medium, be placed in 25 DEG C cultivate 3 days, then be equipped with 100g/L grandson Ah strepto-
The culture dish make-up of bacterium TJGA-19 wheat berry culture (1 method of embodiment is made), sealing continue culture 3 days, then from bacterium
It falls edge and cuts about 8mm × 5mm × 3mm fungus block, each processing cuts 3 ferfas blocks, and 4% glutaraldehyde for putting into pre-cooling immediately is solid
Determine liquid, 4 DEG C of low temperature are stayed overnight, and SEM sample preparation is used for；Mycelium is put into pre-cooling 2.5% penta 2 is gently scraped with sterile toothpick
It is pre-fixed in aldehyde, is used for TEM sample preparation.It is control with the sterile blank wheat berry of equivalent.
SEM sample preparation: sample (uses 0.1molL in 4% glutaraldehyde fixer-1The phosphate buffer that pH is 7.2
Prepare) in 4 DEG C of low temperature stay overnight, use 0.1molL-1The phosphate buffer that pH is 7.2 rinses 3 times, each 15min, after 1% osmic acid
Gu 1h, the dehydration of alcohol for being successively 30%, 50%, 70%, 80% and 90% with concentration, each 10min, then with 100% wine
Twice, each 10min, finally twice with isoamyl acetate transition, each 15min, critical point drying glue sample, cross film for essence dehydration
LEO-1530VP scanning electron microscope is used afterwards, is observed and is taken pictures at 5KV.
TEM sample preparation: sample (uses 0.1molL in 2.5% glutaraldehyde fixer-1The phosphoric acid buffer that p H is 7.2
Liquid is prepared) in 4 DEG C of low temperature stay overnight, use 0.1molL-1The phosphate buffer that pH is 7.2 rinses 3 times, each 15min, 1% osmic acid
Solid afterwards, 4 DEG C of low temperature are stayed overnight, dehydration of alcohol, finally use epoxy infiltration, embedding, and ultramicrotome (EM UC7, Leica) is cut
At 70nm ultra-thin section, the double dyeing of acetic acid uranium lead citrate, transmission electron microscope (Tecnai, FEI) carries out in hyphal cell in 100kV
The observation of portion's structure is simultaneously taken pictures.
Scanning electron microscope the results show that Peronophythora Litchii mycelium is uniform in control treatment, elongated, surface is smooth, full,
Sporangium is spherical, full, cell wall is smooth (Fig. 8 a, b, c)；When Peronophythora Litchii mycelium is exposed to 100g/L grandson Ah streptomycete
In the escaping gas that TJGA-19 wheat berry culture generates, mycelium collapses, is shrivelled, and sporangium cell wall is coarse, sagging
(Fig. 8 d, e, f).Transmission electron microscope results show that control sporangium cytoplasm is uniform, and organelle arrangement is whole, and karyomorphism is normal
(Fig. 9 a, b, c)；Handle Mitochondria, hypochromatosis in the wheat culture of 100g/L, vacuole increase, increase (Fig. 9 d, e,
f)。
Control efficiency of 7 volatile materials of embodiment to excised leaf lichee frost epidemic disease
Sterile big culture dish (H=30mm bottom layer overlay filter paper)It is filtering
The moisturizing of 8mL sterile water is sprayed on paper, places the culture dish bottom of a diameter 90mm in filter paper center, different number is housed in culture dish
Grandson's Ah streptomycete TJGA-19 wheat berry culture (1 method of embodiment be made), 10 blades are then layered on filter paper outer rim,
Blade back upward, takes 2 μ L sporangia suspensions (2 × 104A sporangium/mL) it drips on piece arteries and veins, big culture dish is sealed immediately.It is placed in
Scab length is counted after cultivating 48h under the conditions of 25 DEG C.Experimental setup 4 processing, grandson's Ah streptomycete TJGA-19 wheat berry culture
Amount be respectively 8g/L, 16g/L, 24g/L, 32g/L, be control with the sterile blank wheat berry of equivalent.30 blades of each processing,
3 repetitions of each processing, totally 90 blades, experiment are repeated 3 times.The result is shown in Figure 1 0- Figure 11.
The result shows that: the volatile materials that grandson Ah streptomycete TJGA-19 is generated has very well excised leaf lichee frost epidemic disease
Preventive effect.48h after inoculation, the lesion diameter of control are 26.7mm, when grandson's Ah streptomycete TJGA-19 wheat berry culture amount from
8g/L is increased to 32g/L, and lesion diameter drops to 3mm from 21.9mm.
The antimicrobial spectrum measurement for the volatile materials that 8 grandson Ah streptomycete TJGA-19 of embodiment is generated
It will be inoculated into PDA culture medium for examination pathogen, 6d is cultivated in 25 DEG C of constant incubators and then uses punchBacteria cake is produced in bacterium colony outer rim, fresh PDA plate center is placed in, weighs 32g/L grandson's Ah streptomycete TJGA-19 wheat
Grain culture (preparation method is referring to embodiment 1) moves into 90mm culture dish, is then buckled together two ware bottoms relatively, seals,
Do the device of ware make-up in pairs, for pathogen upper, wheat berry culture is control with the sterile wheat berry of 32g/L under.It will be double
Ware is placed in 25 DEG C of incubators buckle device and cultivates, and observes daily, measurement when each pathogen control bacterium colony covers with culture dish
The diameter (crossing method) of inhibition zone, calculates inhibiting rate, 3 repetitions of each processing, and experiment is repeated 3 times.It the results are shown in Table 2.
Mycelial growth inhibition rate (%)=(control colony diameter-processing colony diameter)/control colony diameter × 100%
Table 2: the antimicrobial spectrum for the volatile materials that grandson Ah streptomycete TJGA-19 is generated
The experimental results showed that the volatile materials that grandson Ah streptomycete TJGA-19 is generated is able to suppress various plants pathogen
Growth, wherein to Peronophythora Litchii, Phytophthora capsici, taro phytophthora, P. myriotylum, Alternaria, fusarium graminearum, banana anthrax
Germ, rice blast fungus, the inhibiting effect of grape ulcer bacterium and papaya Streptomyces scabies are most strong, inhibiting rate up to 63~
100%.
The above content is specific embodiment is combined, further detailed description of the invention, and it cannot be said that this hair
Bright specific implementation is only limited to these instructions.For those of ordinary skill in the art to which the present invention belongs, it is not taking off
Under the premise of from present inventive concept, a number of simple deductions or replacements can also be made.
Claims (7)
1. application of the volatile materials that grandson Ah streptomycete generates in control of plant disease.
2. application according to claim 1, which is characterized in that the plant disease be by Peronophythora Litchii, Phytophthora capsici,
Taro phytophthora, P. myriotylum, Alternaria, fusarium graminearum, Glorosprium musarum Cookeet Mass, rice blast fungus, grape ulcer bacterium
Or plant disease caused by papaya Streptomyces scabies.
3. application according to claim 1 or 2, which is characterized in that the volatile materials includes following component: arsenious acid
Three (trimethyl silicon substrate) esters, the different borneol of 2- methyl, octamethylcy-clotetrasiloxane, tridecane, dimethyl lignocerane, hexyloxy benzoic acid
Ester, 2,6,10- methyl dodecane, the tetradecane, cis- -7,8- epoxy -2- methyl octadecane, 2,6,10- methyltridec, positive two
Octadecane, n-pentadecane, hexadecane and longifolene.
4. application according to claim 3, which is characterized in that the volatile materials is for inhibiting Peronophythora Litchii mycelia
Growth, sporangium yield and egg spore yield.
5. the preparation method of volatile materials as claimed in claim 3, which comprises the following steps:
S1: grandson's Ah streptomycete bacterial strain is crossed on PDA plate culture 7d, after scraping Fresh spores access ISP2 culture medium, is set
In shaken cultivation on shaking table, spore suspension is then prepared, is inoculated on wheat berry culture medium and cultivates, obtains grandson Ah streptomycete wheat
Grain culture；
S2: wheat culture is put into bottle, after sealing is placed, extraction.
6. preparation method according to claim 5, which is characterized in that in the step S1: grandson's Ah streptomycete bacterial strain is existed
Scribing line culture 7d on PDA plate, scraping Fresh spores access in the triangular flask containing 100mL ISP2 culture medium, are subsequently placed in and shake
On bed, with 200rpm, 28 DEG C of shaken cultivation 3d, concentration 1 × 10 is prepared7The spore suspension of a spore/mL, by 1mL/100g's
Ratio is inoculated on wheat berry culture medium, after shaking up, 20d is cultivated under the conditions of being placed in 25 DEG C, every 3d shakes wheat culture medium therebetween
It is even primary.
7. preparation method according to claim 5, which is characterized in that step S2: weighing the grandson Ah streptomycete wheat of culture 20d
Grain culture is put into bottle, places 12h after sealing at 25 DEG C, is then extracted with SPME extracting head.
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| CN110301455B (en) | 2020-09-08 |
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